CN108887088A - The formula and its method and preparation method of a kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation - Google Patents
The formula and its method and preparation method of a kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention discloses a kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, hickory chick Cultivar culture medium, hickory chick base-material, hickory chick nutrient bag.The present invention cultivates the formula of this five kinds of culture mediums by the hickory chick production of hybrid seeds, improves the Morciiella Esculeuta Mycelia speed of growth, mycelia growth is vigorous, and anti-hybrid ability is strong, high survival rate.Culture medium cost reduces, and is conducive to vast cultivation family and receives.Also, locality formula resource is very abundant, and mushroom grower can make full use of local resources to develop hickory chick production, improves hickory chick yield, increases vast cultivation user's economic benefit.
Description
Technical field
The present invention relates to a kind of edible mushroom artificial cultivation techniques, more particularly, to a kind of five kinds of trainings of hickory chick production of hybrid seeds cultivation
The formula and its method and preparation method of feeding base.
Background technique
Original mother culture media is largely：Potato（Peeling）200 grams, 20 grams of agar, 20 grams of glucose, 1000 milli of water
It rises, PH:6.5.The sparse inability of original formulation mycelia, slow growth.
Hickory chick cultivation, first has to select excellent hickory chick kind.Our, the hickory chick of breeding in the end of the year 2016 of original seed factory
Excellent variety " Lu's duty 2 " is chosen as second-class prize of the achievement by the Luzhi County State Scientific and Technological Commission（There is certificate）.Selection kind, it is necessary to first choose hickory chick
Parent species formula, hickory chick original seed formula, the formula of hickory chick cultivar.In addition cultivation is chosen according to local natural resources
The base material formulation of hickory chick chooses the formula of the cultivation secondary nutrient bag of hickory chick and chooses the modeling of each style hickory chick cultivation
Expect greenhouse：Such as：70 centimeters of height canopies, 2 meters high of medium-sized canopy and 4-5 meters of large-diameters cultivate canopy, just can ensure that hickory chick cultivation reaches
To high-quality, high yield.So releasing formula and its method and the preparation side of a kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation
Method, to effectively improve hickory chick yield.
Summary of the invention
The present invention provides the formula and its method and preparation method of a kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, can
Effectively to solve the problems in above-mentioned background technique.
Technical solution of the present invention is summarized as follows：
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The morchella mother culture culture medium includes major ingredient：150-500 grams, powdered glucose：20 grams, soybean powder：20 grams, biphosphate
Potassium：3 grams, magnesium sulfate：1.5 grams, agar：20 grams, vitamin B：4 milligrams, water：1000 milliliters, allotment pH value is 7.5；
The hickory chick Cultivar culture medium includes wheat bran：4%-24.5%, land plaster：1%, pulverized limestone：1%, the hickory chick is planted
Cultivating culture medium further includes boiling swollen wheat berry：70%, corncob：35%-75%, oak consider 10%-60%, humus soil to be worth doing：2.5%-
14%, white sugar：1%-2% or calcium superphosphate：One or more of 0-2%, allotment humidity are 60%-65%, and allotment pH value is 7.5；
The hickory chick Cultivar culture medium includes wheat bran：10%-20%, land plaster：1%, pulverized limestone：1%, the hickory chick cultivation
Kind culture medium further includes well-done wheat berry：50%, oak is considered to be worth doing：20%-80%, humus soil：3%-18%, corn flour：70%, it crushes
Cornstalk：74%, cotton seed hulls：One or more of 74% and white sugar 0-4%, allotment humidity are 65%, and allotment pH value is 7.5；
The hickory chick base-material includes pulverized limestone：1%, land plaster：1%, the hickory chick base-material further includes hardwood sawdust：83%,
Rice bran：15%, crushing maize core：43%-53%, crushing mushroom culture：45% and crush cornstalk：One of 55% or several
Kind, allotment humidity is 65%, and allotment pH value is 7.5；
The hickory chick nutrient bag includes wheat bran:8%-20%, land plaster 1%, the hickory chick nutrient bag further includes broadleaf tree
Bits:35%-80%, crushing cornstalk:45%, cotton seed hulls:40%, corncob:70%, the soybean bar crushed:77%, lime
Powder:1%, humic grows soil:8%-9%, land plaster 1%, calcium superphosphate 1% and white sugar:One or more of 1%, deploy humidity
It is 65%, allotment pH value is 7.5.
Preferably, the major ingredient in the morchella mother culture culture medium is peeled potatoes：200 grams, oak bits liquor：
150 grams, cotton seed hulls liquor：150 grams or yellow bean sprout liquor：One or more of 500 grams.
A kind of production method of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, the production method of morchella mother culture culture medium is such as
Under：1000 milliliters of clear water are added in the major ingredient in morchella mother culture culture medium, boils 30 minutes, is taken with four layers of filtered through gauze
Then agar is added in juice, continue to be heated to whole thawings, glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B, soya bean is added
Powder, and the moisture of evaporation is complemented to 1000 milliliters, pH value is adjusted to 7.5, is hot filling into 18 × 180 milliliters of test tubes, every
Test tube charge weight is 1 ∕ 4-1 ∕ 5, tampon beyond the Great Wall, every 10 a bundle, be put into high-pressure sterilizing pot sterilizing；The morchella mother culture culture
The sterilizing methods of base are：High-pressure sterilizing pot heating, pressure indication arrange cold air when rising to 0.05 pound, return to 0.00 pound to pointer
Afterwards, it is continuously heating to 1.5 pounds, maintains 40 minutes, allow autoclave natural cooling later, take out test tube while hot later, put into oblique
Face is that culture medium length accounts for about 2 ∕ 3 of test tube length and is advisable；
Hickory chick Cultivar culture medium the production method is as follows：After mixing by the formula of hickory chick Cultivar culture medium, add
Enter clear water, when making moisture content in medium up to 65%, can bottle, bottling require it is elastic consistent, cannot be too tight, being filled to flat shoulder is advisable,
Surface is flattened again, washes away the inside and outside culture medium being infected with of bottle wall, then tampon seals beyond the Great Wall；The hickory chick Cultivar culture medium
Sterilizing methods be：High pressure sterilization maintains 2 hours, 100 DEG C of normal-pressure sterilization holding 6-8 hours, to cold under 1.5 pounds of pressure
But it to 28 DEG C, takes out spare；
The hickory chick Cultivar culture medium the production method is as follows：It will be formulated after mixing, water content is made to be maintained at 65%,
PH is adjusted to that 7.5 carry out are bottled, and high pressure sterilization such as uses normal-pressure sterilization, to temperature with the sterilizing methods of morchella mother culture culture medium
It after degree rises to 100 DEG C, is kept for 8-10 hours, is down to 28 DEG C or less to temperature and starts to be inoculated with, be inoculated in inoculating hood, be inoculated with
Case disinfection is lighted stifling 30 minutes with " Babysafe disinfectant powder " 5g/m3, is then inoculated with, 10-15 DEG C of environment is placed on after inoculation
In, it cultivates 20-25 days, after mycelia covers with bottom of bottle, so that it may be directly used in cultivation；
The hickory chick base-material the production method is as follows：It is weighed up in proportion by formula material, then plus clear water is mixed and is uniformly packed into 30
It is centimetre wide, in the woven bag of 60 centimeter lengths, rear tying is filled, is then placed in normal-pressure steam furnace sterilizing, temperature rises to 100 DEG C
Afterwards, 18 hours are kept；
The hickory chick nutrient bag the production method is as follows：After formula material is mixed well, clear water mixing is added, makes compost
It when water content reaches 65%, can pack, the selection of the nutrient bag specification should use high density low pressure polyethylene transparent plastic bag
Or polypropylene dog-ear bag, thickness are generally 0.03-0.05 thickness, specification is that the bag of 12 centimetres wide × 24 centimeter lengths is best, can also
With wide 28 centimetres of the sack of 14 cm x, the compost mixed to be fitted into polybag, it is desirable that it is elastic appropriate, after installing, prick
It sterilizes into sack；The sterilizing methods of the hickory chick nutrient bag are as follows：Generally using local method sterilizing or two kinds of high pressure sterilization
Method, the steaming stove of local method sterilizing have to sealing tightly, reduce to the greatest extent and steam, after temperature rises to 100 DEG C, continue holding 8
It more than a hour, takes out spare after cooling, according to autoclaving, need to be kept for 2-3 hours, be cooled down under 1.5 pounds of pressure
After take out it is spare.
Preferably, army commander's separation of the morchella mother culture culture medium mainly takes tissue point by strict sterilization
From or soil partition method；The Mother culture of the morchella mother culture culture medium is：After the separation of tripe starter kind, strictly purified,
Then it is placed under the conditions of 15-18 DEG C, is protected from light culture, starts to sprout after 1-2 days, cover with slant medium within 7-8 days, it, will after growing up to
Morchella mother culture is put into 1-5 DEG C of refrigerator and saves；
The inoculation of the hickory chick Cultivar culture medium and cultural method are as follows：It is inoculated in the inoculating hood by disinfection,
Every parent species can connect 2-3 bottles of original seed, cultivate under the conditions of being placed on 15-18 DEG C after inoculation, and next day mycelia can start germination and growth, training
It supports 15-25 days, mycelia can cover with bottom of bottle, avoid strong light stimulus and temperature excessively high as far as possible in the training period, can make within general 30 days
With；
The method of nutrient bag is put in the hickory chick nutrient bag：After nutrient bag is cooling, sack is transported into hickory chick cultivation area, it will
Sack is pressed into flat, and acanthopore 12-15 on sack, the nutrient bag for pricking hole, hole mouth down is placed on the bacterium bed of hickory chick cultivation
On.50 centimeter squares put a nutrient bag.It is the pass in hickory chick cultivation in key that cultivation hickory chick, which puts secondary nutrient bag,
Key is an important factor for being related to how many fruiting and yield height.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention cultivates this five kinds cultures by the hickory chick production of hybrid seeds
The formula of base improves the Morciiella Esculeuta Mycelia speed of growth, and mycelia growth is vigorous, and anti-hybrid ability is strong, high survival rate.Culture medium cost
It reduces, is conducive to vast cultivation family and receives.Also, locality formula resource is very abundant, and mushroom grower can make full use of local resources to develop
Hickory chick production, improves hickory chick yield, increases vast cultivation user's economic benefit.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments, based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment one
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The morchella mother culture culture medium includes：Peeled potatoes：200 grams, powdered glucose：20 grams, soybean powder：20 grams, phosphoric acid
Potassium dihydrogen：3 grams, magnesium sulfate：1.5 grams, agar：20 grams, vitamin B：4 milligrams, water：1000 milliliters, pH value：7.5.
A kind of production method of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, the production method of morchella mother culture culture medium is such as
Under：Potato decortication is cleaned, and thinly slices, 1000 milliliters of clear water are added, boil 30 minutes, take juice with four layers of filtered through gauze, then
Agar is added, continues to be heated to whole thawings, powdered glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B, soybean powder is added, and
The moisture of evaporation is complemented to 1000 milliliters, pH value is adjusted to 7.5, is hot filling into 18 × 180 milliliters of test tubes, every cuvette cartridge
Entering amount is 1 ∕ 4-1 ∕ 5, tampon beyond the Great Wall, every 10 a bundle, be put into high-pressure sterilizing pot sterilizing；The morchella mother culture culture medium goes out
Bacterium method is：High-pressure sterilizing pot heating, pressure indication arranges cold air when rising to 0.05 pound, after pointer returns to 0.00 pound, after
It is continuous to be warming up to 1.5 pounds, it maintains 40 minutes, allows autoclave natural cooling later, take out test tube while hot later, put into inclined-plane, be
2 ∕ 3 that culture medium length accounts for about test tube length are advisable.
Embodiment two
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The morchella mother culture culture medium includes：Oak considers liquor to be worth doing：150 grams, glucose：20 grams, agar：20 grams, biphosphate
Potassium：3 grams, magnesium sulfate：1.5, vitamin B：4 milligrams, water：1000 milliliters, allotment pH value is 7.5.
A kind of production method of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, the production method of morchella mother culture culture medium is such as
Under：1000 milliliters of clear water are added after considering liquor to be worth doing in oak, boil 30 minutes, take juice with four layers of filtered through gauze, agar is then added, after
It is continuous to be heated to whole thawings, powdered glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added, and the moisture of evaporation is complemented to
1000 milliliters, pH value is adjusted to 7.5, is hot filling into 18 × 180 milliliters of test tubes, every test tube charge weight is 1 ∕ 4-1 ∕ 5, beyond the Great Wall
Tampon, every 10 a bundle, be put into high-pressure sterilizing pot sterilizing；The sterilizing methods of the morchella mother culture culture medium are：High pressure sterilization
Pot heating, pressure indication arranges cold air when rising to 0.05 pound, after pointer returns to 0.00 pound, is continuously heating to 1.5 pounds, maintains
It 40 minutes, allows autoclave natural cooling later, takes out test tube while hot later, put into inclined-plane, be that culture medium length accounts for about test tube
2 ∕ 3 of length are advisable.
Embodiment three
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The morchella mother culture culture medium includes：Cotton seed hulls liquor：150 grams, glucose：20 grams, agar：20 grams, magnesium sulfate：
1.5 grams, vitamin B：4 milligrams, potassium dihydrogen phosphate：3 grams, water：1000 milliliters, allotment pH value is 7.5.
A kind of production method of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, the production method of morchella mother culture culture medium is such as
Under：1000 milliliters of clear water are added after cotton seed hulls liquor, boils 30 minutes, takes juice with four layers of filtered through gauze, agar is then added, after
It is continuous to be heated to whole thawings, powdered glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added, and the moisture of evaporation is complemented to
1000 milliliters, pH value is adjusted to 7.5, is hot filling into 18 × 180 milliliters of test tubes, every test tube charge weight is 1 ∕ 4-1 ∕ 5, beyond the Great Wall
Tampon, every 10 a bundle, be put into high-pressure sterilizing pot sterilizing；The sterilizing methods of the morchella mother culture culture medium are：High pressure sterilization
Pot heating, pressure indication arranges cold air when rising to 0.05 pound, after pointer returns to 0.00 pound, is continuously heating to 1.5 pounds, maintains
It 40 minutes, allows autoclave natural cooling later, takes out test tube while hot later, put into inclined-plane, be that culture medium length accounts for about test tube
2 ∕ 3 of length are advisable.
Example IV
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The morchella mother culture culture medium includes：Yellow bean sprout liquor：500 grams, powdered glucose：20 grams, agar：20 grams, di(2-ethylhexyl)phosphate
Hydrogen potassium：3 grams, magnesium sulfate：1.5 grams, vitamin B：4 milligrams, water：1000 milliliters, allotment pH value is 7.5.
A kind of production method of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, the production method of morchella mother culture culture medium is such as
Under：1000 milliliters of clear water are added after yellow bean sprout liquor, boils 30 minutes, takes juice with four layers of filtered through gauze, agar is then added, after
It is continuous to be heated to whole thawings, powdered glucose, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added, and the moisture of evaporation is complemented to
1000 milliliters, pH value is adjusted to 7.5, is hot filling into 18 × 180 milliliters of test tubes, every test tube charge weight is 1 ∕ 4-1 ∕ 5, beyond the Great Wall
Tampon, every 10 a bundle, be put into high-pressure sterilizing pot sterilizing；The sterilizing methods of the morchella mother culture culture medium are：High pressure sterilization
Pot heating, pressure indication arranges cold air when rising to 0.05 pound, after pointer returns to 0.00 pound, is continuously heating to 1.5 pounds, maintains
It 40 minutes, allows autoclave natural cooling later, takes out test tube while hot later, put into inclined-plane, be that culture medium length accounts for about test tube
2 ∕ 3 of length are advisable.
Embodiment five
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes boiling swollen wheat berry：70%, oak is considered to be worth doing：10%, wheat bran：4%, humus soil：
14%, land plaster：1%, pulverized limestone：1%, allotment humidity is 60%-65%, and allotment pH value is 7.5.
Hickory chick Cultivar culture medium the production method is as follows：The formula of hickory chick Cultivar culture medium is uniformly mixed
Afterwards, clear water is added, when making moisture content in medium up to 65%, can bottle, bottling require it is elastic consistent, cannot be too tight, be filled to flat shoulder
It is advisable, then surface is flattened, washes away the inside and outside culture medium being infected with of bottle wall, then tampon seals beyond the Great Wall；The hickory chick cultivar
The sterilizing methods of culture medium are：High pressure sterilization maintains 2 hours, 100 DEG C of holding 6-8 of normal-pressure sterilization are small under 1.5 pounds of pressure
When, 28 DEG C are cooled to, is taken out spare.
Embodiment six
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes oak bits：35%, corncob（The small beans size of crushing）：35%, wheat bran：
24.5%, white sugar：1%, land plaster：1%, pulverized limestone 1%, humus soil：2.5%, allotment humidity is 60%-65%, and allotment pH value is
7.5。
Hickory chick Cultivar culture medium the production method is as follows：The formula of hickory chick Cultivar culture medium is uniformly mixed
Afterwards, clear water is added, when making moisture content in medium up to 65%, can bottle, bottling require it is elastic consistent, cannot be too tight, be filled to flat shoulder
It is advisable, then surface is flattened, washes away the inside and outside culture medium being infected with of bottle wall, then tampon seals beyond the Great Wall；The hickory chick cultivar
The sterilizing methods of culture medium are：High pressure sterilization maintains 2 hours, 100 DEG C of holding 6-8 of normal-pressure sterilization are small under 1.5 pounds of pressure
When, 28 DEG C are cooled to, is taken out spare.
Embodiment seven
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes corncob（It is ground into beans size）：75%, wheat bran:20%, land plaster：
1%, pulverized limestone：1%, white sugar：1% ± 2%, allotment humidity is 60%-65%, and allotment pH value is 7.5.
Hickory chick Cultivar culture medium the production method is as follows：The formula of hickory chick Cultivar culture medium is uniformly mixed
Afterwards, clear water is added, when making moisture content in medium up to 65%, can bottle, bottling require it is elastic consistent, cannot be too tight, be filled to flat shoulder
It is advisable, then surface is flattened, washes away the inside and outside culture medium being infected with of bottle wall, then tampon seals beyond the Great Wall；The hickory chick cultivar
The sterilizing methods of culture medium are：High pressure sterilization maintains 2 hours, 100 DEG C of holding 6-8 of normal-pressure sterilization are small under 1.5 pounds of pressure
When, 28 DEG C are cooled to, is taken out spare.
Embodiment eight
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes oak bits：60%, beans bar（It crushes）：20%, wheat bran：15%, white sugar：1%, gypsum
Powder：1%, calcium superphosphate：1% ± 1%, lime：1%, allotment humidity is 60%-65%, and allotment pH value is 7.5.
Hickory chick Cultivar culture medium the production method is as follows：The formula of hickory chick Cultivar culture medium is uniformly mixed
Afterwards, clear water is added, when making moisture content in medium up to 65%, can bottle, bottling require it is elastic consistent, cannot be too tight, be filled to flat shoulder
It is advisable, then surface is flattened, washes away the inside and outside culture medium being infected with of bottle wall, then tampon seals beyond the Great Wall；The hickory chick cultivar
The sterilizing methods of culture medium are：High pressure sterilization maintains 2 hours, 100 DEG C of holding 6-8 of normal-pressure sterilization are small under 1.5 pounds of pressure
When, 28 DEG C are cooled to, is taken out spare.
Embodiment nine
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes wheat berry（It is well-done not have the white heart）：50%, oak is considered to be worth doing：20%, wheat bran：10%, humic
Matter soil：18%, land plaster：1%, lime：1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick Cultivar culture medium the production method is as follows：It will be formulated after mixing, and be maintained at water content
65%, PH are adjusted to that 7.5 carry out are bottled, and high pressure sterilization such as uses normal-pressure sterilization with the sterilizing methods of morchella mother culture culture medium,
After temperature rises to 100 DEG C, is kept for 8-10 hours, is down to 28 DEG C or less to temperature and starts to be inoculated with, be inoculated in inoculating hood,
Inoculating hood disinfection is lighted stifling 30 minutes with " Babysafe disinfectant powder " 5g/m3, is then inoculated with, 10-15 DEG C is placed on after inoculation
In environment, cultivate 20-25 days, after mycelia covers with bottom of bottle, so that it may be directly used in cultivation.
Embodiment ten
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes corn flour（It is crushed to small beans size）：70%, land plaster：1%, wheat bran：
15%, humus soil：10%, pulverized limestone：1%, calcium superphosphate：2%, corncob：1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick Cultivar culture medium the production method is as follows：It will be formulated after mixing, and be maintained at water content
65%, PH are adjusted to that 7.5 carry out are bottled, and high pressure sterilization such as uses normal-pressure sterilization with the sterilizing methods of morchella mother culture culture medium,
After temperature rises to 100 DEG C, is kept for 8-10 hours, is down to 28 DEG C or less to temperature and starts to be inoculated with, be inoculated in inoculating hood,
Inoculating hood disinfection is lighted stifling 30 minutes with " Babysafe disinfectant powder " 5g/m3, is then inoculated with, 10-15 DEG C is placed on after inoculation
In environment, cultivate 20-25 days, after mycelia covers with bottom of bottle, so that it may be directly used in cultivation.
Embodiment 11
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes oak bits：70%, wheat bran：20%, white sugar：1%, land plaster：1%, lime：1%,
Calcium superphosphate：1%, humus soil：6%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick Cultivar culture medium the production method is as follows：It will be formulated after mixing, and be maintained at water content
65%, PH are adjusted to that 7.5 carry out are bottled, and high pressure sterilization such as uses normal-pressure sterilization with the sterilizing methods of morchella mother culture culture medium,
After temperature rises to 100 DEG C, is kept for 8-10 hours, is down to 28 DEG C or less to temperature and starts to be inoculated with, be inoculated in inoculating hood,
Inoculating hood disinfection is lighted stifling 30 minutes with " Babysafe disinfectant powder " 5g/m3, is then inoculated with, 10-15 DEG C is placed on after inoculation
In environment, cultivate 20-25 days, after mycelia covers with bottom of bottle, so that it may be directly used in cultivation.
Embodiment 12
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes oak bits：80%, wheat bran：15%, white sugar：1%, land plaster：1% ± 2%, lime
Powder：1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick Cultivar culture medium the production method is as follows：It will be formulated after mixing, and be maintained at water content
65%, PH are adjusted to that 7.5 carry out are bottled, and high pressure sterilization such as uses normal-pressure sterilization with the sterilizing methods of morchella mother culture culture medium,
After temperature rises to 100 DEG C, is kept for 8-10 hours, is down to 28 DEG C or less to temperature and starts to be inoculated with, be inoculated in inoculating hood,
Inoculating hood disinfection is lighted stifling 30 minutes with " Babysafe disinfectant powder " 5g/m3, is then inoculated with, 10-15 DEG C is placed on after inoculation
In environment, cultivate 20-25 days, after mycelia covers with bottom of bottle, so that it may be directly used in cultivation.
Embodiment 13
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes cornstalk（It crushes）：74%, wheat bran：20%, land plaster：1%, white sugar：1%±
3%, lime powder：1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick Cultivar culture medium the production method is as follows：It will be formulated after mixing, and be maintained at water content
65%, PH are adjusted to that 7.5 carry out are bottled, and high pressure sterilization such as uses normal-pressure sterilization with the sterilizing methods of morchella mother culture culture medium,
After temperature rises to 100 DEG C, is kept for 8-10 hours, is down to 28 DEG C or less to temperature and starts to be inoculated with, be inoculated in inoculating hood,
Inoculating hood disinfection is lighted stifling 30 minutes with " Babysafe disinfectant powder " 5g/m3, is then inoculated with, 10-15 DEG C is placed on after inoculation
In environment, cultivate 20-25 days, after mycelia covers with bottom of bottle, so that it may be directly used in cultivation.
Embodiment 14
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick Cultivar culture medium includes cotton seed hulls：74%, wheat bran：20%, land plaster：1%, white sugar：1%, humus soil：
3%, pulverized limestone：1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick Cultivar culture medium the production method is as follows：It will be formulated after mixing, and be maintained at water content
65%, PH are adjusted to that 7.5 carry out are bottled, and high pressure sterilization such as uses normal-pressure sterilization with the sterilizing methods of morchella mother culture culture medium,
After temperature rises to 100 DEG C, is kept for 8-10 hours, is down to 28 DEG C or less to temperature and starts to be inoculated with, be inoculated in inoculating hood,
Inoculating hood disinfection is lighted stifling 30 minutes with " Babysafe disinfectant powder " 5g/m3, is then inoculated with, 10-15 DEG C is placed on after inoculation
In environment, cultivate 20-25 days, after mycelia covers with bottom of bottle, so that it may be directly used in cultivation.
Embodiment 15
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick base-material includes hardwood sawdust：83%, rice bran：15%, humidity：65%, pulverized limestone：1%, land plaster：1%, it adjusts
It is 65% with humidity, allotment pH value is 7.5.
The hickory chick base-material the production method is as follows：It is weighed up in proportion by formula material, then plus clear water mixes uniform dress
Enter 30 centimetres wide, in the woven bag of 60 centimeter lengths, fill rear tying, be then placed in normal-pressure steam furnace sterilizing, temperature rises to 100
After DEG C, kept for 18 hours.
Embodiment 16
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick base-material includes corncob（It is crushed to small beans size）：45%, hardwood sawdust：53%, pulverized limestone：1%,
Deploying humidity is 65%, and allotment pH value is 7.5.
The hickory chick base-material the production method is as follows：It is weighed up in proportion by formula material, then plus clear water mixes uniform dress
Enter 30 centimetres wide, in the woven bag of 60 centimeter lengths, fill rear tying, be then placed in normal-pressure steam furnace sterilizing, temperature rises to 100
After DEG C, kept for 18 hours.
Embodiment 17
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick base-material includes crushing maize core：53%, mushroom culture bag（Mushroom bag is crossed out）：45%（It crushes）, land plaster:
1%, pulverized limestone：1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick base-material the production method is as follows：It is weighed up in proportion by formula material, then plus clear water mixes uniform dress
Enter 30 centimetres wide, in the woven bag of 60 centimeter lengths, fill rear tying, be then placed in normal-pressure steam furnace sterilizing, temperature rises to 100
After DEG C, kept for 18 hours.
Embodiment 18
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick base-material includes the cornstalk crushed：55%, corncob（It is crushed to small beans size）：43%, land plaster：
1%, pulverized limestone：1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick base-material the production method is as follows：It is weighed up in proportion by formula material, then plus clear water mixes uniform dress
Enter 30 centimetres wide, in the woven bag of 60 centimeter lengths, fill rear tying, be then placed in normal-pressure steam furnace sterilizing, temperature rises to 100
After DEG C, kept for 18 hours.
Embodiment 19
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick nutrient bag includes hardwood sawdust:80%, wheat bran:19%, land plaster:1%, allotment humidity is 65%,
Deploying pH value is 7.5.
The hickory chick nutrient bag the production method is as follows：After formula material is mixed well, clear water mixing is added, makes to train
It when nutriment water content reaches 65%, can pack, the selection of the nutrient bag specification should use the transparent modeling of high density low pressure polyethylene
Material bag or polypropylene dog-ear bag, thickness are generally 0.03-0.05 thickness, and specification is that the bag of 12 centimetres wide × 24 centimeter lengths is best,
Wide 28 centimetres of the sack of 14 cm x can also be used, the compost mixed is fitted into polybag, it is desirable that it is elastic appropriate, it installs
Afterwards, it pricks and sterilizes into sack；The sterilizing methods of the hickory chick nutrient bag are as follows：Generally gone out using local method sterilizing or high pressure
Two methods of bacterium, the steaming stove of local method sterilizing has to sealing tightly, reduces to the greatest extent and steam, and after temperature rises to 100 DEG C, continues
It is kept for 8 more than hour, takes out spare after cooling, according to autoclaving, need to kept for 2-3 hours under 1.5 pounds of pressure,
It is taken out after cooling spare.
Embodiment 20
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick nutrient bag includes cornstalk（It crushes）:45%, hardwood sawdust:45%, wheat bran:8%, land plaster:
1%, pulverized limestone:1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick nutrient bag the production method is as follows：After formula material is mixed well, clear water mixing is added, makes to train
It when nutriment water content reaches 65%, can pack, the selection of the nutrient bag specification should use the transparent modeling of high density low pressure polyethylene
Material bag or polypropylene dog-ear bag, thickness are generally 0.03-0.05 thickness, and specification is that the bag of 12 centimetres wide × 24 centimeter lengths is best,
Wide 28 centimetres of the sack of 14 cm x can also be used, the compost mixed is fitted into polybag, it is desirable that it is elastic appropriate, it installs
Afterwards, it pricks and sterilizes into sack；The sterilizing methods of the hickory chick nutrient bag are as follows：Generally gone out using local method sterilizing or high pressure
Two methods of bacterium, the steaming stove of local method sterilizing has to sealing tightly, reduces to the greatest extent and steam, and after temperature rises to 100 DEG C, continues
It is kept for 8 more than hour, takes out spare after cooling, according to autoclaving, need to kept for 2-3 hours under 1.5 pounds of pressure,
It is taken out after cooling spare.
Embodiment 21
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick nutrient bag includes cotton seed hulls:40%, hardwood sawdust:35%, wheat bran:15%, humic grows soil:9%,
Land plaster 1%, allotment humidity are 65%, and allotment pH value is 7.5.
The hickory chick nutrient bag the production method is as follows：After formula material is mixed well, clear water mixing is added, makes to train
It when nutriment water content reaches 65%, can pack, the selection of the nutrient bag specification should use the transparent modeling of high density low pressure polyethylene
Material bag or polypropylene dog-ear bag, thickness are generally 0.03-0.05 thickness, and specification is that the bag of 12 centimetres wide × 24 centimeter lengths is best,
Wide 28 centimetres of the sack of 14 cm x can also be used, the compost mixed is fitted into polybag, it is desirable that it is elastic appropriate, it installs
Afterwards, it pricks and sterilizes into sack；The sterilizing methods of the hickory chick nutrient bag are as follows：Generally gone out using local method sterilizing or high pressure
Two methods of bacterium, the steaming stove of local method sterilizing has to sealing tightly, reduces to the greatest extent and steam, and after temperature rises to 100 DEG C, continues
It is kept for 8 more than hour, takes out spare after cooling, according to autoclaving, need to kept for 2-3 hours under 1.5 pounds of pressure,
It is taken out after cooling spare.
Embodiment 22
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick nutrient bag includes corncob（Crushing or small beans size）:70%, wheat bran 19%, humic grow soil:8%,
Land plaster 1%, calcium superphosphate 1%, pulverized limestone 1%, allotment humidity are 65%, and allotment pH value is 7.5.
The hickory chick nutrient bag the production method is as follows：After formula material is mixed well, clear water mixing is added, makes to train
It when nutriment water content reaches 65%, can pack, the selection of the nutrient bag specification should use the transparent modeling of high density low pressure polyethylene
Material bag or polypropylene dog-ear bag, thickness are generally 0.03-0.05 thickness, and specification is that the bag of 12 centimetres wide × 24 centimeter lengths is best,
Wide 28 centimetres of the sack of 14 cm x can also be used, the compost mixed is fitted into polybag, it is desirable that it is elastic appropriate, it installs
Afterwards, it pricks and sterilizes into sack；The sterilizing methods of the hickory chick nutrient bag are as follows：Generally gone out using local method sterilizing or high pressure
Two methods of bacterium, the steaming stove of local method sterilizing has to sealing tightly, reduces to the greatest extent and steam, and after temperature rises to 100 DEG C, continues
It is kept for 8 more than hour, takes out spare after cooling, according to autoclaving, need to kept for 2-3 hours under 1.5 pounds of pressure,
It is taken out after cooling spare.
Embodiment 23
A kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep tripe
Bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, including as following formula：
The hickory chick nutrient bag includes soybean bar（It crushes）:77%, wheat bran:20%, white sugar:1%, land plaster:1%, lime
Powder:1%, allotment humidity is 65%, and allotment pH value is 7.5.
The hickory chick nutrient bag the production method is as follows：After formula material is mixed well, clear water mixing is added, makes to train
It when nutriment water content reaches 65%, can pack, the selection of the nutrient bag specification should use the transparent modeling of high density low pressure polyethylene
Material bag or polypropylene dog-ear bag, thickness are generally 0.03-0.05 thickness, and specification is that the bag of 12 centimetres wide × 24 centimeter lengths is best,
Wide 28 centimetres of the sack of 14 cm x can also be used, the compost mixed is fitted into polybag, it is desirable that it is elastic appropriate, it installs
Afterwards, it pricks and sterilizes into sack；The sterilizing methods of the hickory chick nutrient bag are as follows：Generally gone out using local method sterilizing or high pressure
Two methods of bacterium, the steaming stove of local method sterilizing has to sealing tightly, reduces to the greatest extent and steam, and after temperature rises to 100 DEG C, continues
It is kept for 8 more than hour, takes out spare after cooling, according to autoclaving, need to kept for 2-3 hours under 1.5 pounds of pressure,
It is taken out after cooling spare.
The formula that this five kinds of culture mediums are cultivated by the hickory chick production of hybrid seeds, improves the Morciiella Esculeuta Mycelia speed of growth, and mycelia is raw
Length is vigorous, and anti-hybrid ability is strong, high survival rate.Culture medium cost reduces, and is conducive to vast cultivation family and receives.Also, locality formula money
Source is very abundant, and mushroom grower can make full use of local resources to develop hickory chick production, improves hickory chick yield, increases vast cultivation
User's economic benefit.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown here.
Claims (4)
1. a kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, including morchella mother culture culture medium, hickory chick pedigree seed culture medium, sheep
Tripe bacteria cultivation kind culture medium, hickory chick base-material, hickory chick nutrient bag, which is characterized in that including as following formula：
The morchella mother culture culture medium includes major ingredient：150-500 grams, powdered glucose：20 grams, soybean powder：20 grams, biphosphate
Potassium：3 grams, magnesium sulfate：1.5 grams, agar：20 grams, vitamin B：4 milligrams, water：1000 milliliters, allotment pH value is 7.5；
The hickory chick Cultivar culture medium includes wheat bran：4%-24.5%, land plaster：1%, pulverized limestone：1%, the hickory chick is planted
Cultivating culture medium further includes boiling swollen wheat berry：70%, corncob：35%-75%, oak consider 10%-60%, humus soil to be worth doing：2.5%-
14%, white sugar：1%-2% or calcium superphosphate：One or more of 0-2%, allotment humidity are 60%-65%, and allotment pH value is 7.5；
The hickory chick Cultivar culture medium includes wheat bran：10%-20%, land plaster：1%, pulverized limestone：1%, the hickory chick cultivation
Kind culture medium further includes well-done wheat berry：50%, oak is considered to be worth doing：20%-80%, humus soil：3%-18%, corn flour：70%, it crushes
Cornstalk：74%, cotton seed hulls：One or more of 74% and white sugar 0-4%, allotment humidity are 65%, and allotment pH value is 7.5；
The hickory chick base-material includes pulverized limestone：1%, land plaster：1%, the hickory chick base-material further includes hardwood sawdust：83%,
Rice bran：15%, crushing maize core：43%-53%, crushing mushroom culture：45% and crush cornstalk：One of 55% or several
Kind, allotment humidity is 65%, and allotment pH value is 7.5；
The hickory chick nutrient bag includes wheat bran:8%-20%, land plaster 1%, the hickory chick nutrient bag further includes broadleaf tree
Bits:35%-80%, crushing cornstalk:45%, cotton seed hulls:40%, corncob:70%, the soybean bar crushed:77%, lime
Powder:1%, humic grows soil:8%-9%, land plaster 1%, calcium superphosphate 1% and white sugar:One or more of 1%, deploy humidity
It is 65%, allotment pH value is 7.5.
2. a kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation according to claim 1, which is characterized in that the sheep tripe
Major ingredient in bacterium mother culture media is peeled potatoes：200 grams, oak bits liquor：150 grams, cotton seed hulls liquor：150 grams or
Yellow bean sprout liquor：One or more of 500 grams.
3. a kind of production method of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation, which is characterized in that morchella mother culture culture medium
The production method is as follows：1000 milliliters of clear water are added in the major ingredient in morchella mother culture culture medium, boil 30 minutes, with four layers of yarn
Cloth filters to take juice, and agar is then added, and continues to be heated to whole thawings, and glucose, potassium dihydrogen phosphate, magnesium sulfate, dimension life is added
Plain B, soybean powder, and the moisture of evaporation is complemented to 1000 milliliters, pH value is adjusted to 7.5, is hot filling into 18 × 180 milliliters of test tubes
In, every test tube charge weight is 1 ∕ 4-1 ∕ 5, beyond the Great Wall tampon, every 10 a bundle, be put into high-pressure sterilizing pot sterilizing；The hickory chick
The sterilizing methods of mother culture media are：High-pressure sterilizing pot heating, pressure indication arrange cold air when rising to 0.05 pound, return to pointer
To after 0.00 pound, it is continuously heating to 1.5 pounds, maintains 40 minutes, allow autoclave natural cooling later, takes out test tube while hot later,
Inclined-plane is put into, is that culture medium length accounts for about 2 ∕ 3 of test tube length and is advisable；
Hickory chick Cultivar culture medium the production method is as follows：After mixing by the formula of hickory chick Cultivar culture medium, add
Enter clear water, when making moisture content in medium up to 65%, can bottle, bottling require it is elastic consistent, cannot be too tight, being filled to flat shoulder is advisable,
Surface is flattened again, washes away the inside and outside culture medium being infected with of bottle wall, then tampon seals beyond the Great Wall；The hickory chick Cultivar culture medium
Sterilizing methods be：High pressure sterilization maintains 2 hours, 100 DEG C of normal-pressure sterilization holding 6-8 hours, to cold under 1.5 pounds of pressure
But it to 28 DEG C, takes out spare；
The hickory chick Cultivar culture medium the production method is as follows：It will be formulated after mixing, water content is made to be maintained at 65%,
PH is adjusted to that 7.5 carry out are bottled, and high pressure sterilization such as uses normal-pressure sterilization, to temperature with the sterilizing methods of morchella mother culture culture medium
It after degree rises to 100 DEG C, is kept for 8-10 hours, is down to 28 DEG C or less to temperature and starts to be inoculated with, be inoculated in inoculating hood, be inoculated with
Case disinfection " Babysafe disinfectant powder " 5g/m3It lights stifling 30 minutes, is then inoculated with, 10-15 DEG C of environment is placed on after inoculation
In, it cultivates 20-25 days, after mycelia covers with bottom of bottle, so that it may be directly used in cultivation；
The hickory chick base-material the production method is as follows：It is weighed up in proportion by formula material, then plus clear water is mixed and is uniformly packed into 30
It is centimetre wide, in the woven bag of 60 centimeter lengths, rear tying is filled, is then placed in normal-pressure steam furnace sterilizing, temperature rises to 100 DEG C
Afterwards, 18 hours are kept；
The hickory chick nutrient bag the production method is as follows：After formula material is mixed well, clear water mixing is added, makes compost
It when water content reaches 65%, can pack, the selection of the nutrient bag specification should use high density low pressure polyethylene transparent plastic bag
Or polypropylene dog-ear bag, thickness are generally 0.03-0.05 thickness, specification is that the bag of 12 centimetres wide × 24 centimeter lengths is best, can also
With wide 28 centimetres of the sack of 14 cm x, the compost mixed to be fitted into polybag, it is desirable that it is elastic appropriate, after installing, prick
It sterilizes into sack；The sterilizing methods of the hickory chick nutrient bag are as follows：Generally using local method sterilizing or two kinds of high pressure sterilization
Method, the steaming stove of local method sterilizing have to sealing tightly, reduce to the greatest extent and steam, after temperature rises to 100 DEG C, continue holding 8
It more than a hour, takes out spare after cooling, according to autoclaving, need to be kept for 2-3 hours, be cooled down under 1.5 pounds of pressure
After take out it is spare.
4. a kind of production method of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation according to claim 3, feature exist
In army commander's separation of the morchella mother culture culture medium mainly takes tissue separation or soil separation by strict sterilization
Method；The Mother culture of the morchella mother culture culture medium is：After the separation of tripe starter kind, is strictly purified, be then placed on 15-18
Under the conditions of DEG C, it is protected from light culture, starts to sprout after 1-2 days, covers with slant medium within 7-8 days, after growing up to, morchella mother culture is put into
It is saved in 1-5 DEG C of refrigerator；
The inoculation of the hickory chick Cultivar culture medium and cultural method are as follows：It is inoculated in the inoculating hood by disinfection,
Every parent species can connect 2-3 bottles of original seed, cultivate under the conditions of being placed on 15-18 DEG C after inoculation, and next day mycelia can start germination and growth, training
It supports 15-25 days, mycelia can cover with bottom of bottle, avoid strong light stimulus and temperature excessively high as far as possible in the training period, can make within general 30 days
With；
The method of nutrient bag is put in the hickory chick nutrient bag：After nutrient bag is cooling, sack is transported into hickory chick cultivation area, it will
Sack is pressed into flat, and acanthopore 12-15 on sack, the nutrient bag for pricking hole, hole mouth down is placed on the bacterium bed of hickory chick cultivation
On,
50 centimeter squares put a nutrient bag, and it is the pass in hickory chick cultivation in key that cultivation hickory chick, which puts secondary nutrient bag,
Key is an important factor for being related to how many fruiting and yield height.
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| CN113170702A (en) * | 2020-08-18 | 2021-07-27 | 山西大同大学 | Multi-season morchella planting method and application thereof |
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| CN112400607A (en) * | 2020-10-14 | 2021-02-26 | 安徽乐永园农业科技有限公司 | Preparation and use methods of culture medium suitable for culturing morchella |
| CN112772277A (en) * | 2021-01-20 | 2021-05-11 | 广西民族师范学院 | Ecological morchella planting method |
| CN112931052A (en) * | 2021-04-02 | 2021-06-11 | 甘肃省科学院生物研究所 | Morchella cultivation medium and preparation method thereof |
| CN113243248A (en) * | 2021-06-15 | 2021-08-13 | 重庆市城口县松坤菌草科技开发有限责任公司 | Morchella strain cultivation method |
| CN114395488A (en) * | 2022-01-26 | 2022-04-26 | 辽宁省农业科学院 | Culture medium for promoting growth of mushroom mononuclear hyphae and preparation method thereof |
| CN114395488B (en) * | 2022-01-26 | 2024-05-17 | 辽宁省农业科学院 | Culture medium for promoting growth of single-core mycelia of lentinus edodes and preparation method thereof |
| CN115362889A (en) * | 2022-07-08 | 2022-11-22 | 宁夏农林科学院农业生物技术研究中心（宁夏农业生物技术重点实验室） | Method for interplanting strawberry and morchella |
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