CN112400607A - Preparation and use methods of culture medium suitable for culturing morchella - Google Patents
Preparation and use methods of culture medium suitable for culturing morchella Download PDFInfo
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- CN112400607A CN112400607A CN202011093636.7A CN202011093636A CN112400607A CN 112400607 A CN112400607 A CN 112400607A CN 202011093636 A CN202011093636 A CN 202011093636A CN 112400607 A CN112400607 A CN 112400607A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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Abstract
The invention discloses a preparation method and a use method of a culture medium suitable for culturing morchella esculenta, and the preparation method comprises the preparation of a morchella esculenta culture medium, wherein the morchella esculenta culture medium comprises 5-15% of poplar root additives, 25-40% of raw potatoes, 10-15% of glucose, 20-30% of agar and the balance of water by weight.
Description
Technical Field
The invention belongs to the technical field of bioengineering, particularly relates to the technical field of biological plant culture, and particularly relates to a preparation method and a use method of a culture medium for morchella planting and breeding.
Background
The morchella is a precious edible fungus and medicinal fungus, and contains 20% of crude protein, 26% of crude fat, 38.1% of carbohydrate, various amino acids and vitamins, and particularly contains 1.76% of glutamic acid. Clinical research of modern medicine shows that the morchella has the effects of improving immunity, resisting tumor and resisting aging and has higher application value.
At the present stage, due to the fact that the economic value of morchella is high and the morchella is suitable for popularization, a plurality of farmers begin to plant the morchella, however, the strain selection of the morchella is very important, the yield of breeding and cultivation of the morchella is not high all the time in the breeding aspect of the morchella, therefore, the artificial planting of the morchella is restricted, the situations of low breeding survival rate, more mixed bacteria and the like exist all the time, and the method also becomes a condition for restricting the popularization of the morchella planting technology.
Disclosure of Invention
In order to solve the problems in the background art, the preparation method and the use method of the culture medium suitable for culturing the morchella are provided, the culture medium for breeding is developed by researching the growth habit and the environment of the morchella, the culture medium prepared by the method for breeding can effectively improve the strain amount and the excellence of sclerotia, and a better solution is provided for removing mixed bacteria.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method and a use method of a culture medium suitable for culturing morchella esculenta comprise the step of preparing a culture medium for culturing morchella esculenta, wherein the culture medium for culturing morchella esculenta comprises 5-15 wt% of a poplar root additive, 25-40 wt% of raw potatoes, 10-15 wt% of glucose, 20-30 wt% of agar and the balance water.
As a preferred technical scheme, the raw potatoes are undeveloped potatoes, and the preparation process of the poplar root additive comprises the steps of putting poplar roots into water, boiling, filtering the poplar roots after boiling, taking water and cooling.
As a preferred technical scheme, the preparation process of the bacteria culture medium comprises the following steps:
A. taking a plurality of grams of poplar roots, cleaning and crushing the poplar roots, pouring the crushed poplar roots into water, boiling the poplar roots, keeping the poplar roots for a period of time, cooling the poplar roots, and filtering the poplar roots to obtain a soup serving as an additive of the poplar roots;
B. taking water, crushing raw potatoes, putting the crushed raw potatoes into water, adding agar strips, boiling and boiling the agar strips with slow fire for a period of time until the agar strips are completely melted and the potatoes are boiled to be in a greasy and unbreakable state, adding glucose, stirring, completely dissolving to obtain an intermediate, and cooling for later use;
C. mixing the poplar root additive prepared in the step A and the intermediate prepared in the step B in proportion, fully boiling, and fully stirring and mixing to obtain a culture medium;
D. c, subpackaging the culture medium prepared in the step C into clean test tubes, sealing the test tubes by using sterile cotton, uniformly placing the test tubes into an autoclave, carrying out autoclaving, sterilizing for a period of time, cutting off the power supply, waiting for the pressure of the autoclave to be zero, and taking out the test tubes to obtain a culture medium to be molded;
E. placing the culture medium to be formed prepared in the step D at an inclination of 7-10 degrees until the culture medium inclined plane in the test tube does not touch the aseptic cotton plug, placing the test tube in a heat preservation box, and keeping for 7 days to solidify the test tube to obtain a culture medium test tube;
F. and E, observing the culture medium test tube prepared in the step E, wherein the color is faint yellow to golden yellow, and obtaining the bacteria culture medium.
As a preferable technical scheme, the distance of the aseptic cotton seal in the step D is greater than 1/10 and less than 1/5 of the length of the test tube.
As a preferable technical scheme, the sterilization time in the step D is more than 2.5H and less than 3H.
As a preferred technical scheme, the temperature range of the heat preservation box in the step E is 25-30 ℃.
As a preferable technical scheme, the weight ratio of the poplar root powder to water is 1: 2.5-1: 3, and the weight ratio of the filtered poplar root to the obtained soup after boiling is 1: 2-1: 2.5.
Compared with the prior art, this beneficial effect is: through the study on the habits of morchella, the morchella is found to have the characteristic of being fond of yin, and the growth environment of morchella in Shaanxi, Gansu, Guangxi, Yunnan, Jilin, Hubei and Anhui, Suzhou is combined, so that the technology is developed, the strain quantity and the superiority of sclerotia can be effectively improved, the unit quantity of strains is improved, the cost is reduced, and an effective method and basis are provided for the large-scale cultivation of morchella.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the preparation process of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The preparation process of the bacteria culture medium comprises the following steps:
A. taking a plurality of grams of poplar roots, cleaning and crushing the poplar roots, pouring the crushed poplar roots into water, boiling the poplar roots, keeping the poplar roots for a period of time, cooling the poplar roots, and filtering the poplar roots to obtain a soup serving as an additive of the poplar roots;
B. taking water, crushing raw potatoes, putting the crushed raw potatoes into water, adding agar strips, boiling and boiling the agar strips with slow fire for a period of time until the agar strips are completely melted and the potatoes are boiled to be in a greasy and unbreakable state, adding glucose, stirring, completely dissolving to obtain an intermediate, and cooling for later use;
C. mixing the poplar root additive prepared in the step A and the intermediate prepared in the step B in proportion, fully boiling, and fully stirring and mixing to obtain a culture medium;
D. c, subpackaging the culture medium prepared in the step C into clean test tubes, sealing the test tubes by using sterile cotton, uniformly placing the test tubes into an autoclave, carrying out autoclaving, sterilizing for a period of time, cutting off the power supply, waiting for the pressure of the autoclave to be zero, and taking out the test tubes to obtain a culture medium to be molded;
E. placing the culture medium to be formed prepared in the step D at an inclination of 7-10 degrees until the culture medium inclined plane in the test tube does not touch the aseptic cotton plug, placing the test tube in a heat preservation box, and keeping for 7 days to solidify the test tube to obtain a culture medium test tube;
F. and E, observing the culture medium test tube prepared in the step E, wherein the color is faint yellow to golden yellow, and obtaining the bacteria culture medium.
Specifically, the distance between the aseptic cotton seals in the step D is greater than 1/10 and less than 1/5 of the length of the test tube.
Specifically, the sterilization time in the step D is more than 2.5H and less than 3H.
Specifically, the temperature range of the heat preservation box in the step E is 25-30 ℃.
Specifically, the weight ratio of the poplar root powder to water is 1: 2.5-1: 3, and the weight ratio of the filtered poplar root to the obtained soup after boiling is 1: 2-1: 2.5.
Example 1
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises 5% of raw potatoes, 25% of raw potatoes, 15% of glucose, 25% of agar and the balance of water by weight. (70)
Example 2
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 10% of the poplar root additive, 25% of raw potatoes, 15% of glucose, 25% of agar and the balance water. (75)
Example 3
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 15% of the poplar root additive, 25% of the raw potatoes, 15% of the glucose, 25% of the agar and the balance of water. (80)
Example 4
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 10% of the poplar root additive, 40% of raw potatoes, 10% of the glucose, 20% of the agar and the balance of water. (80)
Example 5
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 10% of the poplar root additive, 30% of raw potatoes, 10% of glucose, 20% of agar and the balance water. (70)
Example 6
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 10% of the poplar root additive, 25% of raw potatoes, 10% of the glucose, 30% of the agar and the balance of water. (75)
Example 7
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 15% of the poplar root additive, 30% of raw potatoes, 10% of glucose, 30% of agar and the balance water. (85)
Example 8
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 15% of the poplar root additive, 30% of raw potatoes, 15% of glucose, 30% of agar and the balance water. (90)
Example 9
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 15% of the poplar root additive, 30% of raw potatoes, 15% of the glucose, 25% of the agar and the balance of water. (85)
Example 10
A preparation method and a use method of a culture medium suitable for culturing morchella comprise the preparation of a culture medium for culturing morchella, wherein the culture medium for culturing morchella comprises raw potatoes, glucose, agar and a poplar root additive, and comprises the following components, by weight, 15% of the poplar root additive, 30% of raw potatoes, 15% of the glucose, 20% of the agar and the balance of water. (80)
Example 11
Boiling green potato into a greasy and unbreakable state, and preparing a culture medium; labeled as style 1;
example 12
Boiling germinated potato to obtain a greasy and unbreakable state, and preparing a culture medium; labeled as style 2;
example 13
Boiling normal potato to obtain a greasy and unbreakable state, and preparing a culture medium; labeled as style 3;
example 14
Taking fresh poplar leaves, crushing and cooking the fresh poplar leaves, and preparing a culture medium under the same other conditions as in example 3; labeled as style 4;
example 15
Taking fresh branches and trunks of the poplar, crushing and cooking the branches and trunks to prepare a culture medium, wherein other conditions are the same as those in the embodiment 3; labeled as style 5;
example 16
Taking soil under poplar, fully stirring, soaking in water for precipitation, taking water, and replacing poplar root additive; a medium was prepared under the same conditions as in example 3; labeled as style 6;
the morchella spores are cultured on the different culture mediums respectively, and 5 parts of each spore are prepared, so that the following list is obtained:
note: it can be seen that the formulation according to example 3 is excellent.
Note: it was found that the compounding ratio in example 13 is excellent.
Note: it can be seen that the formulation according to example 3 is excellent, and the formulations according to other examples 14, 15 and 16 are not good, and particularly, the replacement of poplar leaves has the worst effect for culturing morchella, the replacement of poplar roots with soil has a good effect, but the mixed bacteria are easy to occur, and the formulations are not as good as those of example 3 in other aspects.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The preparation and use method of the culture medium suitable for culturing the morchella esculenta is characterized by comprising the step of preparing a culture medium for culturing the morchella esculenta, wherein the culture medium for culturing the morchella esculenta comprises 5-15% of poplar root additive, 25-40% of raw potatoes, 10-15% of glucose, 20-30% of agar and the balance of water in parts by weight.
2. The preparation and use method of the culture medium for Morchella esculenta cultivation according to claim 1, wherein the culture medium comprises: the raw potato is undeveloped potato, and the poplar root additive is prepared by boiling poplar root in water, filtering the poplar root after boiling, taking water and cooling.
3. The preparation and use method of the culture medium for Morchella esculenta cultivation according to claim 1, wherein the culture medium comprises: the preparation process of the bacteria culture medium comprises the following steps:
taking a plurality of grams of poplar roots, cleaning and crushing the poplar roots, pouring the crushed poplar roots into water, boiling the poplar roots, keeping the poplar roots for a period of time, cooling the poplar roots, and filtering the poplar roots to obtain a soup serving as an additive of the poplar roots;
taking water, crushing raw potatoes, putting the crushed raw potatoes into water, adding agar strips, boiling and boiling the agar strips with slow fire for a period of time until the agar strips are completely melted and the potatoes are boiled to be in a greasy and unbreakable state, adding glucose, stirring, completely dissolving to obtain an intermediate, and cooling for later use;
mixing the poplar root additive prepared in the step A and the intermediate prepared in the step B in proportion, fully boiling, and fully stirring and mixing to obtain a culture medium;
c, subpackaging the culture medium prepared in the step C into clean test tubes, sealing the test tubes by using sterile cotton, uniformly placing the test tubes into an autoclave, carrying out autoclaving, sterilizing for a period of time, cutting off the power supply, waiting for the pressure of the autoclave to be zero, and taking out the test tubes to obtain a culture medium to be molded;
placing the culture medium to be formed prepared in the step D at an inclination of 7-10 degrees until the culture medium inclined plane in the test tube does not touch the aseptic cotton plug, placing the test tube in a heat preservation box, and keeping for 7 days to solidify the test tube to obtain a culture medium test tube;
and E, observing the culture medium test tube prepared in the step E, wherein the color is faint yellow to golden yellow, and obtaining the bacteria culture medium.
4. The preparation and use method of the culture medium suitable for Morchella esculenta cultivation according to claim 3, wherein the culture medium comprises: the distance between the aseptic cotton seals in the step D is greater than 1/10 and less than 1/5 of the length of the test tube.
5. The preparation and use method of the culture medium suitable for Morchella esculenta cultivation according to claim 3, wherein the culture medium comprises: the sterilization time in the step D should be more than 2.5H and less than 3H.
6. The preparation and use method of the culture medium suitable for Morchella esculenta cultivation according to claim 3, wherein the culture medium comprises: and E, controlling the temperature of the heat preservation box to be 25-30 ℃.
7. The preparation and use method of the culture medium for Morchella esculenta cultivation according to claim 2, wherein the culture medium comprises: the weight ratio of the poplar root powder to water is 1: 2.5-1: 3, and the weight ratio of the filtered poplar root to the obtained soup after boiling is 1: 2-1: 2.5.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113455287A (en) * | 2021-07-07 | 2021-10-01 | 重庆市酉阳县琦睿峰食用菌有限责任公司 | Method for directly separating morchella tissue by PDA (personal digital Assistant) plate to obtain pure strain |
| CN113597975A (en) * | 2021-09-01 | 2021-11-05 | 绵阳市经科菌业有限责任公司 | Preparation method of high-yield culture medium for morchella cultivars |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113597975A (en) * | 2021-09-01 | 2021-11-05 | 绵阳市经科菌业有限责任公司 | Preparation method of high-yield culture medium for morchella cultivars |
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