CN113597975A - Preparation method of high-yield culture medium for morchella cultivars - Google Patents
Preparation method of high-yield culture medium for morchella cultivars Download PDFInfo
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- CN113597975A CN113597975A CN202111021341.3A CN202111021341A CN113597975A CN 113597975 A CN113597975 A CN 113597975A CN 202111021341 A CN202111021341 A CN 202111021341A CN 113597975 A CN113597975 A CN 113597975A
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- morchella
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- 239000001963 growth medium Substances 0.000 title claims abstract description 42
- 241000221638 Morchella Species 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 235000012343 cottonseed oil Nutrition 0.000 claims abstract description 21
- 239000010902 straw Substances 0.000 claims abstract description 21
- 238000003756 stirring Methods 0.000 claims abstract description 20
- 235000015097 nutrients Nutrition 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 17
- 240000002769 Morchella esculenta Species 0.000 claims abstract description 13
- 235000002779 Morchella esculenta Nutrition 0.000 claims abstract description 13
- 210000003608 fece Anatomy 0.000 claims abstract description 13
- 239000002689 soil Substances 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 12
- 239000002023 wood Substances 0.000 claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims abstract description 9
- 230000004151 fermentation Effects 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 241000219000 Populus Species 0.000 claims description 7
- 239000000654 additive Substances 0.000 claims description 7
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- 230000000996 additive effect Effects 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 4
- 239000012257 stirred material Substances 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 2
- 230000000050 nutritive effect Effects 0.000 abstract description 4
- 241000894007 species Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 9
- 239000000203 mixture Substances 0.000 description 8
- 241000233866 Fungi Species 0.000 description 7
- 238000011161 development Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 208000019790 abdominal distention Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 244000309146 drought grass Species 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the technical field of cultivation, and discloses a preparation method of a morchella esculenta cultivated species high-yield culture medium, which comprises the following steps of preparing materials, namely 2-5 parts of wood chips, 20-30 parts of fermented cow dung, 1-3 parts of cottonseed hulls, 1-3 parts of straws, 5-15 parts of wheat bran, 1-3 parts of rape stalks, 0.1-0.5 part of soil and 2-3 parts of nutrient water, crushing, namely crushing the cottonseed hulls into 1-3cm, crushing the straws into 1-3cm, crushing the rape stalks into 1-3cm, stirring, mixing and fermenting, taking 3 parts of wood chips, 25 parts of fermented cow dung, 2 parts of cottonseed hulls, 2 parts of straws, 10 parts of wheat bran, 2 parts of rape stalks, 0.2 part of soil and 3 parts of nutrient water, stirring, mixing uniformly, standing for fermentation for 2-3 hours, and finally, sterilizing at high temperature to obtain a culture medium, wherein the finished culture medium has high nutritive value and low cost, effectively ensures the normal growth of the morchella and can effectively improve the growth efficiency of the morchella.
Description
Technical Field
The invention belongs to the technical field of cultivation, and particularly relates to a preparation method of a high-yield culture medium for morchella cultivars.
Background
With the development and progress of science and technology, the requirements of people for things are changed from old thinking that people can use things as things to be quite fine and smooth, and only with the continuous progress or innovation conception, a new form is generated to improve the added value of the product, so that people can live under the test of intense market competition. Morchella is a rare edible fungus, which is named because the surface of the cap is uneven and is like morchella. The Morchella is also called Morchella esculenta, and Morchella esculenta. Can be used for treating dyspepsia, qi stagnation, abdominal distention, phlegm stagnation, adverse flow of qi, and cough and asthma. The Morchella is also called Morchella esculenta and Morchella esculenta. Morchella (Morchella), also known as bamboo grass, is a precious edible fungus and medicinal fungus.
After the nutritional value of various fungi is discovered under the rapid development of modern society, the market has a demand for the fungi, but the fungi have low yield in natural growth and strict requirements on the growth environment, and in order to produce the fungi, a culture medium is usually manufactured, wherein the culture medium is an artificially prepared nutrient for providing growth and maintenance of microorganisms, plant tissues and animal tissues, the nutrient in the culture medium is completely suitable for the growth of morchella esculenta, and the environment suitable for the growth of the morchella esculenta is provided through the environmental control in a greenhouse.
The nutrient value of the culture medium in the prior art is not high, so that the growth effect of the morchella is not ideal, and the raw materials for preparing the culture medium are not common, high in price, not easy to produce, or troublesome to prepare.
Disclosure of Invention
In view of the problems raised by the above background art, the present invention is directed to: aims to provide a preparation method of a morchella cultivar high-yield culture medium.
In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:
a preparation method of a morchella cultivar high-yield culture medium comprises the following steps:
s1: preparing materials, namely 2-5 parts of wood chips, 20-30 parts of fermented cow dung, 1-3 parts of cottonseed hulls, 1-3 parts of straws, 5-15 parts of wheat bran, 1-3 parts of rape stems, 0.1-0.5 part of soil and 2-3 parts of nutrient water;
s2: crushing, namely crushing cottonseed hulls into 1-3cm, crushing straws into 1-3cm, and crushing rape stems into 1-3 cm;
s3: stirring, mixing and fermenting, namely uniformly stirring and mixing 3 parts of sawdust, 25 parts of fermented cow dung, 2 parts of cottonseed hulls, 2 parts of straws, 10 parts of wheat bran, 2 parts of rape stalks, 0.2 part of soil and 3 parts of nutrient water, and then standing for 2-3 hours for fermentation;
s4: sterilizing at high temperature to obtain culture medium.
In step S1, the preparation method further includes adding 2-5 parts of poplar root additive, 3-5 parts of lime water solution and 1-4 parts of shell powder, and adding the poplar root additive, the lime water solution and the shell powder into the culture medium can increase the nutritive value of the culture medium.
Further, in the step S1, the wood chips, the cottonseed hulls, and the wheat bran are wetted earlier and left for one week, and the effect is good when the wood chips, the cottonseed hulls, and the wheat bran are mixed for use.
Further, in step S2, the cottonseed hulls, the stalks, the rice straws and the rape stems can be simultaneously crushed, so as to increase the preparation efficiency.
Further limiting, in the step S3, one third of the sawdust, the fermented cow dung, the cottonseed hulls, the straws, the wheat bran, the rape stems, the soil and the nutrient water are added for stirring at one time, the mixture is added for three times, the stirring is carried out for 0.5 to 1min each time, and the mixture is uniformly mixed with good effect.
Further, in step S3, the stirring and forming criteria is to tilt a scoop of the stirring object without any water drop and to ensure the humidity of the stirring object.
Further limiting, in the step S3, the fermented product after fermentation is sealed at 10-20 ℃ to ensure the subsequent use effect.
Further limiting, in the step S4, the fermented product is sterilized in a sterilization device for 3-5 hours, the state of the sterilization device is 100 ℃ at normal pressure, and then the culture medium is obtained by cooling to normal temperature, so as to ensure the sterilization effect.
The invention has the following beneficial effects:
1. the finished product culture medium has high nutritive value and low cost, effectively ensures the normal growth of the morchella and can effectively improve the growth efficiency of the morchella;
2. the invention has the advantages of simple raw material collection, simple preparation method and easy preparation.
Drawings
The invention is further illustrated by the non-limiting examples given in the accompanying drawings;
FIG. 1 is a block diagram of preparation steps of an embodiment of a preparation method of a high-yield medium for Morchella esculenta cultivars according to the invention;
Detailed Description
In order that those skilled in the art can better understand the present invention, the following technical solutions are further described with reference to the accompanying drawings and examples.
As shown in fig. 1, the preparation method of the high-yield culture medium for morchella cultivars of the present invention comprises the following steps:
s1: preparing materials, namely 2-5 parts of wood chips, 20-30 parts of fermented cow dung, 1-3 parts of cottonseed hulls, 1-3 parts of straws, 5-15 parts of wheat bran, 1-3 parts of rape stems, 0.1-0.5 part of soil and 2-3 parts of nutrient water;
s2: crushing, namely crushing cottonseed hulls into 1-3cm, crushing straws into 1-3cm, and crushing rape stems into 1-3 cm;
s3: stirring, mixing and fermenting, namely uniformly stirring and mixing 3 parts of sawdust, 25 parts of fermented cow dung, 2 parts of cottonseed hulls, 2 parts of straws, 10 parts of wheat bran, 2 parts of rape stalks, 0.2 part of soil and 3 parts of nutrient water, and then standing for 2-3 hours for fermentation;
s4: sterilizing at high temperature to obtain culture medium.
In the embodiment, when a preparation method of a morchella esculenta cultivar high-yield culture medium is used, 2-5 parts of prepared wood chips, 20-30 parts of fermented cow dung, 1-3 parts of cotton seed hulls, 1-3 parts of straws, 5-15 parts of wheat bran, 1-3 parts of rape stems, 0.1-0.5 part of soil and 2-3 parts of nutrient water are prepared, and 2-5 parts of poplar root additives, 3-5 parts of lime water solution and 1-4 parts of shell powder can be selected to increase the nutritional value of the culture medium, then the cotton seed hulls are crushed into 1-3cm, the straws are crushed into 1-3cm, the rape stems are crushed into 1-3cm, the crushing mode is not limited, the sequence is not limited as long as the sequence is not required to be too regular within a corresponding range, then 3 parts of wood chips, 25 parts of fermented cow dung, 2 parts of cottonseed hulls, 2 parts of straws, 10 parts of wheat bran, 2 parts of rape stems, 0.2 part of soil and 3 parts of nutrient water are mixed and stirred together, the nutrient water is added in batches during the period, the stirring is stopped during the adding period, no water drops when the mixture is stirred until the mixture is scooped by a spoon and the spoon is inclined, then the mixture is stood for 2 to 3 hours for fermentation, and the mixture is sterilized at high temperature, so that the finished cultivation medium can be obtained.
Preferably, in step S1, the prepared materials further include 2-5 parts of poplar root additive, 3-5 parts of lime water solution, and 1-4 parts of shell powder, and adding the poplar root additive, the lime water solution, and the shell powder into the culture medium can increase the nutritive value of the culture medium, and actually, whether the poplar root additive, the lime water solution, and the shell powder are added or not can be considered according to specific conditions.
Preferably, in step S1, the wood chips, cotton seed hulls, and wheat bran are wetted before being left for one week, and the mixture is used with good effect, and in fact, the time of keeping can be considered according to specific situations.
Preferably, in step S2, the cottonseed hulls, the stalks, the straw, and the rape stems can be simultaneously crushed to increase the preparation efficiency, and actually, the crushing mode can be considered according to specific situations.
Preferably, in the step S3, one third of the sawdust, the fermented cow dung, the cottonseed hulls, the straws, the wheat bran, the rape stems, the soil and the nutrient water are added at a time and stirred for three times, wherein the stirring time is 0.5-1min each time, the mixture is uniformly mixed, the effect is good, and actually, the stirring mode can be considered according to specific conditions.
In step S3, the stirring and forming is preferably performed by tilting a scoop of the stirred material to prevent dripping and ensure the moisture of the stirred material, and actually, the stirring degree may be considered according to specific conditions.
Preferably, in step S3, the fermented product is sealed at 10-20 ℃ to ensure the subsequent use effect, and in fact, the environmental temperature during sealing can be considered according to specific conditions.
Preferably, in step S4, the fermentation product is sterilized in a sterilization apparatus for 3-5 hours, the sterilization apparatus is at 100 ℃ under normal pressure, and then the culture medium is cooled to normal temperature, so as to ensure the sterilization effect.
The foregoing embodiments are merely illustrative of the principles of the present invention and its efficacy, and are not to be construed as limiting the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (8)
1. A preparation method of a high-yield culture medium for morchella cultivars is characterized by comprising the following steps:
s1: preparing materials, namely 2-5 parts of wood chips, 20-30 parts of fermented cow dung, 1-3 parts of cottonseed hulls, 1-3 parts of straws, 5-15 parts of wheat bran, 1-3 parts of rape stems, 0.1-0.5 part of soil and 2-3 parts of nutrient water;
s2: crushing, namely crushing cottonseed hulls into 1-3cm, crushing straws into 1-3cm, and crushing rape stems into 1-3 cm;
s3: stirring, mixing and fermenting, namely uniformly stirring and mixing 3 parts of sawdust, 25 parts of fermented cow dung, 2 parts of cottonseed hulls, 2 parts of straws, 10 parts of wheat bran, 2 parts of rape stalks, 0.2 part of soil and 3 parts of nutrient water, and then standing for 2-3 hours for fermentation;
s4: sterilizing at high temperature to obtain culture medium.
2. The preparation method of the high-yield culture medium for the morchella cultivar according to claim 1, wherein the culture medium comprises the following components in percentage by weight: in the step S1, the prepared materials further comprise 2-5 parts of poplar root additive, 3-5 parts of lime water solution and 1-4 parts of shell powder.
3. The preparation method of the high-yield culture medium for the morchella esculenta cultivar according to claim 2, wherein the culture medium comprises the following components in percentage by weight: in the step S1, the wood chips, the cotton seed hulls and the wheat bran are wetted earlier and left for one week.
4. The preparation method of the high-yield culture medium for the morchella esculenta cultivar according to claim 3, wherein the culture medium comprises the following components in percentage by weight: in the step S2, the cottonseed hulls, the stalks, the rice straws and the rape stems may be simultaneously crushed.
5. The preparation method of the high-yield culture medium for the morchella cultivars according to claim 4, wherein the culture medium comprises the following components in percentage by weight: in the step S3, one third of the sawdust, the fermented cow dung, the cottonseed hulls, the straws, the wheat bran, the rape stems, the soil and the nutrient water are added at one time and stirred for three times, and the stirring time is 0.5 to 1min each time.
6. The preparation method of the high-yield culture medium for the morchella cultivars according to claim 5, wherein the culture medium comprises the following components in percentage by weight: in step S3, the stirring and forming is performed in such a manner that a scoop of the stirred material is tilted and no water drops are observed.
7. The method for preparing a high-yield culture medium for morel cultivars according to claim 6, wherein the culture medium comprises the following components in percentage by weight: in the step S3, the fermented product is sealed at 10-20 deg.C.
8. The method for preparing a high-yield culture medium for morel cultivars according to claim 7, wherein the culture medium comprises the following components in percentage by weight: in the step S4, the fermentation product is sterilized in a sterilization device for 3-5h, the state of the sterilization device is normal pressure 100 ℃, and then the fermentation product is cooled to normal temperature to obtain the culture medium.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106116743A (en) * | 2016-06-15 | 2016-11-16 | 铜仁市侗菇菌业发展有限公司 | A kind of Morchella esculenta (L.) Pers culture medium for cultivating material |
| CN106146090A (en) * | 2016-07-04 | 2016-11-23 | 四川省农业科学院土壤肥料研究所 | Straw stalk fermentation substrate, Morchella esculenta (L.) Pers spawn culture substrate and preparation method thereof |
| CN106927880A (en) * | 2017-02-15 | 2017-07-07 | 广东东阳光药业有限公司 | The culture matrix of indoor growing ladder rib hickory chick and its preparation method and application |
| CN108934778A (en) * | 2018-08-14 | 2018-12-07 | 芜湖野树林生物科技有限公司 | A kind of culture medium and preparation method thereof promoting hickory chick growth |
| CN109496691A (en) * | 2018-11-28 | 2019-03-22 | 湖南金芙农业科技有限公司 | Hickory chick culture substrate and preparation method thereof |
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2021
- 2021-09-01 CN CN202111021341.3A patent/CN113597975A/en active Pending
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Application publication date: 20211105 |