CN103214294A - Secondary fruiting nutrient solution for golden mushroom and preparation method - Google Patents

Secondary fruiting nutrient solution for golden mushroom and preparation method Download PDF

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Publication number
CN103214294A
CN103214294A CN2013100742562A CN201310074256A CN103214294A CN 103214294 A CN103214294 A CN 103214294A CN 2013100742562 A CN2013100742562 A CN 2013100742562A CN 201310074256 A CN201310074256 A CN 201310074256A CN 103214294 A CN103214294 A CN 103214294A
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China
Prior art keywords
needle mushroom
wheat
nutritive medium
water
wheat malt
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CN2013100742562A
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CN103214294B (en
Inventor
颜丙强
巩玉辉
陈翠翠
程韬
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HUBEI FUSHIFENG BIOTECHNOLOGY CO Ltd
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HUBEI FUSHIFENG BIOTECHNOLOGY CO Ltd
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Abstract

The invention discloses a secondary fruiting nutrient solution for factory bottle cultivation golden mushroom, and the nutrient solution contains the following ingredients by weight: 30-40 parts of corn flour, 20-30 parts of wheat malt, 5-15 parts of river mud powder, 10-20 parts of golden mushroom, 0.1-1 parts of KH2PO4, 0.1-1 parts of MgSO4, and 200-400 parts of water. Compared with the one time fruiting, the factory bottle cultivation golden mushroom secondary fruiting of the present invention has the advantages of saved raw material, improved raw material utilization rate, saved inoculation and disinfection and other flows, and increased biotransformation efficiency. In real production, after the first time fruiting, each cultivation bottle is added with 450ml aseptic nutrient solution, and then the secondary fruiting.

Description

A kind of needle mushroom secondary fruiting nutritive medium and preparation method
Technical field
What the present invention relates to is the edible mushrooms technical field, particularly relates to golden mushroom cultivation technique field.
Background technology
The batch production golden mushroom plantation, only pluck mushroom usually one time at present, and the culture material of having plucked is both discarded, in discarded bacterium slag, contains a large amount of nutritive ingredients; In batch production golden mushroom plantation process, the needle mushroom punching is sent out bacteria growing to fruiting and is completed, the nutritive ingredients such as the carbohydrate of cultivation bottle, inorganic salt are consumed in a large number, produce a large amount of enzymes in culture bottle simultaneously, remain a large amount of utilizable Mierocrystalline celluloses, make golden mushroom plantation bottle secondary fruiting under state of nature, but the secondary fruiting is of poor quality, generally all there is no commodity value.
The needle mushroom that batch production is produced, produce a lot of needle mushroom tankage, as golden mushroom root, these tankage do not have edibleness, but contain the nutritive ingredient that a lot of Growth of Flammulina Velutipes can directly absorb, tankage are pulverized and can be recycled according to certain density nutritive medium.
To pass through soil covering culture during present edible mushrooms secondary fruiting, earthing requires high to soil property, and easily cause the later stage needle mushroom to pollute, in industrialized cultivation, can't apply, add the river silt powder in the present invention in nutritive medium, replace earthing, after the river silt sterilizing, can stimulate needle mushroom fruiting, can not cause again needle mushroom to pollute, and application is convenient in industrialized cultivation.
Summary of the invention
The existing industrial bottle of purpose utilization of the present invention is planted secondary fruiting nutritive medium and the preparation technology thereof of the tankage production needle mushroom of needle mushroom fruiting.
Technical scheme of the present invention is:
A kind of industrial bottle is planted needle mushroom secondary fruiting nutritive medium, and the nutritive medium component comprises: Semen Maydis powder, wheat, river silt powder, needle mushroom waste material cream, KH 2pO 4, MgSO 4, water.
Nutritive medium component by weight comprises: Semen Maydis powder 30~40, wheat 20~30, river silt powder 5~15, needle mushroom waste material cream 10~20, KH 2pO 40.1~1, MgSO 40.1~1, water 200~400.
Wherein preferred version is: Semen Maydis powder 38, wheat 27, river silt 10, needle mushroom waste material cream 15, KH 2pO 40.2, MgSO 40.1, H 2o 360.
The preparation of needle mushroom waste material cream dead meal cream: the root that will pluck needle mushroom is collected, and puts into pulverizer according to ratio of water to material 1:1 and breaks into slurries.
The wheat malt preparation method: take in proportion wheat, wheat is mixed according to ratio of water to material 2:1,10~15 ℃, 48h, obtain wheat malt, and wheat malt is pulverized and processed.
Planting needle mushroom secondary fruiting compounding method for a kind of bottle is:
1) preparation of needle mushroom waste material cream dead meal cream: the root that will pluck needle mushroom is collected, and puts into pulverizer according to ratio of water to material 1:1 and breaks into slurries;
2) wheat germ preparation method: take in proportion wheat, wheat is mixed according to ratio of water to material 2:1,10~15 ℃, 48h, obtain wheat malt, and wheat malt is pulverized and processed;
3) by Semen Maydis powder, wheat malt, river silt powder, needle mushroom waste material cream, KH 2pO 4, MgSO 4stir, add water, regulate PH6.5, boil;
4) by the nutritive medium boiled, 121 ℃, 30min sterilizing, cooling.
Industrial bottle is planted the technique that needle mushroom does not have the secondary fruiting at present, but reality is found in producing, bottle is planted needle mushroom for the first time after fruiting, the nutritive ingredient that in bottle, residue much is not utilized, after some sugars of supplementary Growth of Flammulina Velutipes necessity, inorganic salt, culture bottle can be realized the secondary fruiting fully again.
The secondary fruiting generally will carry out soil covering culture, soil covering culture requires high to soil, and easily cause factory's pollution in wide area, the present invention adds river silt in nutritive medium, after high-temperature sterilization, add culture bottle, can play the stimulation needle mushroom fruiting equally, and technique is simple, operational safety, the microbial contamination of having avoided earthing to cause.
Industrial bottle is planted the relative fruiting of needle mushroom secondary fruiting, have and save material, improve utilization rate of raw materials, save the flow processs such as inoculation, sterilizing, biological transformation ratio in raising, in actual production, after fruiting, every culture bottle is added without bacterial nutrient solution 450ml, fruiting 200-250 gram/bottle again, twice comprehensive utilization makes biological transformation ratio bring up to 160-180%, than a fruiting formula, improved 70-80%, and economic benefit is very obvious .
The general formulation cost of batch production needle mushroom is 1.5-2 unit, fruiting 240-280 gram/bottle, the needle mushroom nutritive medium cost of making by this formula is for being about 0.5 yuan, and by adding this nutritive medium fruiting 200-250 gram/bottle again, and incubation time can shorten about 20 days.
The pricing of every 450ml nutritive medium
Embodiment
Embodiment 1
1 industrial bottle is planted needle mushroom secondary fruiting nutritive medium component weight ratio formula: Semen Maydis powder 38; Wheat malt 27; River silt 10; Needle mushroom waste material cream 15; KH 2pO 40.2; MgSO 40.1; H 2o 360;
2. the preparation of needle mushroom waste material cream dead meal cream: the root that will pluck needle mushroom is collected, and puts into pulverizer according to ratio of water to material 1:1 and breaks into slurries.
Wheat germ preparation method: take in proportion wheat, wheat is mixed according to ratio of water to material 2:1,10~15 ℃, 48h, obtain wheat malt, and wheat malt is pulverized and processed.Proportionally Semen Maydis powder, wheat malt, river silt powder, needle mushroom waste material cream are stirred, add KH2PO4, MgSo4, add water and add according to formula, regulate PH6.5, boil.By the nutritive medium boiled, every 450ml pours in the high-temperature resistance plastice bottle, 121 ℃, 30min sterilizing, cooling;
3. after the batch production needle mushroom is plucked for the first time, make mushroom culturing device again clear away the aging tissue that the bacterium bottle shows;
4. inject the 450ml nutritive medium to every culture bottle, the lid lid, proceed to the bacteria room, 25 ℃ of temperature, and humidity 65%~70%, go to fertility chamber fruiting after dark culturing sends out bacterium full, when needle mushroom 15cm, gathers, the average every bottle of 250g that gathers.
Embodiment 2
Industrial bottle is planted needle mushroom secondary fruiting nutritive medium component weight ratio formula: Semen Maydis powder 40; Wheat malt 30; River silt 15; Needle mushroom waste material cream 20; KH 2pO 41; MgSO 41; H 2o 400; Other is identical with example 1, the average every bottle of 230g that gathers.
Embodiment 3
Industrial bottle is planted needle mushroom secondary fruiting nutritive medium component weight ratio formula: Semen Maydis powder 30; Wheat malt 20; River silt 5; Needle mushroom waste material cream 10; KH 2pO 40.1; MgSO 40.1; H 2o 200; Other is identical with example 1, the average every bottle of 200g that gathers.

Claims (9)

1. an industrial bottle is planted needle mushroom secondary fruiting nutritive medium, it is characterized in that, described nutritive medium component comprises: Semen Maydis powder, wheat malt, river silt powder, needle mushroom waste material cream, KH 2pO 4, MgSO 4, water.
2. nutritive medium as claimed in claim 1, is characterized in that, described nutritive medium component by weight is: Semen Maydis powder 30~40, wheat malt 20~30, river silt powder 5~15, needle mushroom waste material cream 10~20, KH 2pO 40.1~1, MgSO 40.1~1, water 200~400.
3. nutritive medium as claimed in claim 1, is characterized in that, described nutritive medium component by weight is: Semen Maydis powder 38, wheat malt 27, river silt 10, needle mushroom waste material cream 15, KH 2pO 40.2, MgSO 40.1; H 2o 360.
4. nutritive medium as claimed in claim 1 or 2, is characterized in that, the preparation of described needle mushroom waste material cream dead meal cream: the root that will pluck needle mushroom is collected, and puts into pulverizer according to ratio of water to material 1:1 and breaks into slurries.
5. nutritive medium as claimed in claim 1 or 2, is characterized in that, described wheat malt preparation method: take in proportion wheat, wheat is mixed according to ratio of water to material 2:1, and 10-15 ℃, 48h, obtain wheat malt, and wheat malt is pulverized and is processed.
6. a bottle is planted needle mushroom secondary fruiting compounding method, it is characterized in that comprising the steps:
1) preparation of needle mushroom waste material cream dead meal cream: the root that will pluck needle mushroom is collected, and puts into pulverizer according to ratio of water to material 1:1 and breaks into slurries;
2) wheat germ preparation method: take in proportion wheat, wheat is mixed according to ratio of water to material 2:1,10~15 ℃, 48h, obtain wheat malt, and wheat malt is pulverized and processed;
3) proportionally by Semen Maydis powder, wheat malt, river silt powder, needle mushroom waste material cream, KH 2pO 4, MgSO 4stir, KH 2pO 4, MgSO 4, add water, regulate PH6.5, boil;
4), by the nutritive medium boiled, temperature is that 121 ℃, time are the 30min sterilizing, cooling.
7. method as claimed in claim 6, is characterized in that, the Semen Maydis powder in described step 3), wheat malt, river silt powder, needle mushroom waste material cream, KH 2pO 4, MgSO 4component is by weight: 30~40,20~30,5~15,10~20,0.1~1,0.1~1.
8. method as described as claim 6 or 7, is characterized in that, the Semen Maydis powder in described step 3), wheat malt, river silt powder, needle mushroom waste material cream, KH 2pO 4, MgSO 4component is by weight: 38,27,10,15,0.2,0.1.
9. method as described as claim 6 or 7, is characterized in that, described step 3) adds water and the material weight ratio is: 3~4:1.
CN201310074256.2A 2013-03-08 2013-03-08 Secondary fruiting nutrient solution for golden mushroom and preparation method Expired - Fee Related CN103214294B (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103460996A (en) * 2013-08-31 2013-12-25 山西省医药与生命科学研究院 Secondary high yield technology of waste mushroom-sticks of pleurotus eryngii
CN103704013A (en) * 2013-12-16 2014-04-09 武义创新食用菌有限公司 Method for cultivating shitake mushrooms by segmental fruiting
CN105016853A (en) * 2015-06-26 2015-11-04 何金霞 Needle mushroom medium and method using medium to cultivate needle mushroom
CN107673854A (en) * 2017-10-27 2018-02-09 广西浙缘农业科技有限公司 A kind of multiple fruiting nutritional supplementation liquid of asparagus and preparation method thereof
CN111557210A (en) * 2020-05-29 2020-08-21 江苏华绿生物科技股份有限公司 Flammulina velutipes root culture medium used for cultivation of flammulina velutipes
CN111699922A (en) * 2020-06-17 2020-09-25 鲁东大学 Needle mushroom strain preservation culture medium, needle mushroom culture material and application thereof
CN112385477A (en) * 2020-11-20 2021-02-23 山东沃尔美肥业有限公司 Secondary fruiting method of needle mushrooms

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US20030121201A1 (en) * 2001-12-04 2003-07-03 Dahlberg Kurt R. Polysaccharide mushroom compost supplements
CN102334417A (en) * 2010-07-28 2012-02-01 上海市农业科学院 Method for cultivating needle mushroom by using liquid nutritional substrate
CN102613004A (en) * 2012-04-14 2012-08-01 河北荣珍食用菌股份有限公司 Method using pleurotus nebrodensis waste to cultivate flammulina velutipes
CN102630485A (en) * 2012-04-16 2012-08-15 何寒 Method for cultivating flammulina velutipes through taking water-retaining agent solidified nutrient solution as culture medium
CN102910949A (en) * 2011-08-06 2013-02-06 天水众兴菌业科技股份有限公司 Flammulina velutipes liquid strain culture medium and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN1043610A (en) * 1989-03-03 1990-07-11 福建农学院 Make the technology of nutritional solution with the JINZHENGU solid culture
US20030121201A1 (en) * 2001-12-04 2003-07-03 Dahlberg Kurt R. Polysaccharide mushroom compost supplements
CN102334417A (en) * 2010-07-28 2012-02-01 上海市农业科学院 Method for cultivating needle mushroom by using liquid nutritional substrate
CN102910949A (en) * 2011-08-06 2013-02-06 天水众兴菌业科技股份有限公司 Flammulina velutipes liquid strain culture medium and preparation method thereof
CN102613004A (en) * 2012-04-14 2012-08-01 河北荣珍食用菌股份有限公司 Method using pleurotus nebrodensis waste to cultivate flammulina velutipes
CN102630485A (en) * 2012-04-16 2012-08-15 何寒 Method for cultivating flammulina velutipes through taking water-retaining agent solidified nutrient solution as culture medium

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103460996A (en) * 2013-08-31 2013-12-25 山西省医药与生命科学研究院 Secondary high yield technology of waste mushroom-sticks of pleurotus eryngii
CN103460996B (en) * 2013-08-31 2016-03-16 山西省医药与生命科学研究院 Pleurotus eryngii waste rod secondary high-yield method
CN103704013A (en) * 2013-12-16 2014-04-09 武义创新食用菌有限公司 Method for cultivating shitake mushrooms by segmental fruiting
CN103704013B (en) * 2013-12-16 2015-09-09 武义创新食用菌有限公司 A kind of method of segmentation fruiting mushroom culture
CN105016853A (en) * 2015-06-26 2015-11-04 何金霞 Needle mushroom medium and method using medium to cultivate needle mushroom
CN107673854A (en) * 2017-10-27 2018-02-09 广西浙缘农业科技有限公司 A kind of multiple fruiting nutritional supplementation liquid of asparagus and preparation method thereof
CN111557210A (en) * 2020-05-29 2020-08-21 江苏华绿生物科技股份有限公司 Flammulina velutipes root culture medium used for cultivation of flammulina velutipes
CN111699922A (en) * 2020-06-17 2020-09-25 鲁东大学 Needle mushroom strain preservation culture medium, needle mushroom culture material and application thereof
CN111699922B (en) * 2020-06-17 2023-03-10 鲁东大学 Needle mushroom strain preservation medium, needle mushroom culture material and application thereof
CN112385477A (en) * 2020-11-20 2021-02-23 山东沃尔美肥业有限公司 Secondary fruiting method of needle mushrooms
CN112385477B (en) * 2020-11-20 2022-06-14 山东沃尔美肥业有限公司 Secondary fruiting method of needle mushrooms

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