CN106116743A - A kind of Morchella esculenta (L.) Pers culture medium for cultivating material - Google Patents
A kind of Morchella esculenta (L.) Pers culture medium for cultivating material Download PDFInfo
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- CN106116743A CN106116743A CN201610424635.3A CN201610424635A CN106116743A CN 106116743 A CN106116743 A CN 106116743A CN 201610424635 A CN201610424635 A CN 201610424635A CN 106116743 A CN106116743 A CN 106116743A
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- culture medium
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- cultivating
- morchella esculenta
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The invention provides a kind of Morchella esculenta (L.) Pers culture medium for cultivating material, belong to planting edible mushroom technical field, including the raw material of following weight portion: 30～50 parts of wood flours, 10～20 parts of Testa Tritici, 10～15 parts of bagasse, 5～15 parts of wormcasts, 3～6 parts of distiller grains, 10～15 parts of straws, 10～15 parts of fertile soil, 2～4 parts of potassium dihydrogen phosphates, 1～2 part of Gypsum Fibrosum.Use the culture medium for cultivating material that the wood flour of the present invention, Testa Tritici, bagasse, wormcast, distiller grains, straw, fertile soil, potassium dihydrogen phosphate, Gypsum Fibrosum are made, it has the advantages that miscellaneous bacteria is few, success rate is high, Morchella esculenta (L.) Pers yield is high, cultivation base stock disclosed in relatively patent documentation 1, the success rate of the present invention can reach more than 95% (high 10～15 percentage points than patent documentation 1), high by more than 50% than patent documentation 1 of the average product of single culture medium for cultivating.
Description
Technical field
The invention belongs to fungus growing technique field, particularly relate to a kind of Morchella esculenta (L.) Pers culture medium for cultivating material.
Background technology
Morchella esculenta (L.) Pers has another name called Gaster caprae seu Ovis dish, for morchella edible fungi, is edible fungi and the medicinal fungus of a kind of preciousness；Morchella esculenta (L.) Pers
Nutrition is the abundantest, Morchella esculenta (L.) Pers containing suppression tumor polysaccharide, antibacterial, the active component of antiviral, have enhancing human body immunity power,
All multiactions such as resisting fatigue, antiviral, suppression tumor；Owing to Morchella esculenta (L.) Pers is a kind of extremely precious wild mushroom, it is possible to as
Food materials are being sold on the market, and therefore Morchella esculenta (L.) Pers needs the large-area plant development that carries out, and keeps the good quality and high output of Morchella esculenta (L.) Pers to need
Want excellent Morchella esculenta (L.) Pers strain.
Present stage, the Cultivating techniques of Morchella esculenta (L.) Pers mainly has thalline to excite, amplification culture, cultigen make (such as patent documentation
1CN104137736A), making at cultigen and need base material, in routine techniques, base material as disclosed in patent documentation 1 is Semen Tritici aestivi
75% boil after pull out, put into 15% dowel, 5% Testa Tritici and 5% earth and stir；It has that miscellaneous bacteria is many, Morchella esculenta (L.) Pers yield relatively
The shortcoming such as few.
Summary of the invention
For solving above-mentioned technical problem, the invention provides a kind of Morchella esculenta (L.) Pers culture medium for cultivating material, concrete scheme is as follows:
A kind of Morchella esculenta (L.) Pers culture medium for cultivating material, including the raw material of following weight portion: 30～50 portions of wood flours, 10～20 portions of wheats
Bran, 10～15 portions of bagasse, 5～15 parts of wormcasts, 3～6 parts of distiller grains, 10～15 parts of straws, 10～15 parts of fertile soil, 2～4 parts
Potassium dihydrogen phosphate, 1～2 part of Gypsum Fibrosum.
Further, including the raw material of following weight portion: 40～50 portions of wood flours, 15～20 portions of Testa Tritici, 10～13 portions of Caulis Sacchari sinensis
Slag, 10～15 parts of wormcasts, 4～6 parts of distiller grains, 10～13 parts of straws, 12～15 parts of fertile soil, 2～4 parts of potassium dihydrogen phosphates, 1～
2 parts of Gypsum Fibrosum.
Further, including the raw material of following weight portion: 45 portions of wood flours, 18 portions of Testa Tritici, 12 portions of bagasse, 13 portions of Lumbricuss
Excrement, 5 parts of distiller grains, 12 parts of straws, 13 parts of fertile soil, 3 parts of potassium dihydrogen phosphates, 1.5 parts of Gypsum Fibrosum.
Further, this culture medium for cultivating material is made by following steps:
1) by crushed stalk, then according to all raw materials are mixed by ratio, in the environment of 25～40 DEG C stack fermentation 3～
6 days；
2) raw material after fermentation is loaded in Polypropylene Bag, and on Polypropylene Bag, open multiple osculum, be placed on autoclave
Middle sterilizing, preserves more than 4h at normal pressure 100 DEG C, taking-up is cooled to room temperature and makes bar-shaped culture medium for cultivating.
The beneficial effects of the present invention is: use the wood flour of the present invention, Testa Tritici, bagasse, wormcast, distiller grains, straw, corruption
Grow soil, culture medium for cultivating material that potassium dihydrogen phosphate, Gypsum Fibrosum are made, it has, and miscellaneous bacteria is few, success rate is high, Morchella esculenta (L.) Pers yield high
Feature, made by the preparation technology of the present invention, it is possible to play more preferable effect simultaneously.The relatively disclosed cultivation of patent documentation 1
Base material, the success rate of the present invention can reach more than 95% (high 10～15 percentage points than patent documentation 1), single cultivation training
Support high by more than 50% than patent documentation 1 of average product of base.
Detailed description of the invention
Technical scheme is described further below, but claimed scope is not limited to described.
Embodiment one
Present embodiments provide a kind of Morchella esculenta (L.) Pers culture medium for cultivating material, including following raw material: 30kg wood flour, 10kg Testa Tritici,
10kg bagasse, 5kg wormcast, 3kg distiller grains, 15kg straw, 15kg fertile soil, 4kg potassium dihydrogen phosphate, 2kg Gypsum Fibrosum.
This culture medium for cultivating material is made by following steps:
1) by crushed stalk, then according to all raw materials are mixed by ratio, in the environment of 25 DEG C, fermentation 6 days is stacked；
2) raw material after fermentation is loaded in Polypropylene Bag, and on Polypropylene Bag, open multiple osculum, be placed on autoclave
Middle sterilizing, preserves more than 4h at normal pressure 100 DEG C, taking-up is cooled to room temperature and makes bar-shaped culture medium for cultivating, culture medium for cultivating
Diameter is less than 10cm, and length is less than 50cm.
Embodiment two
Present embodiments provide a kind of Morchella esculenta (L.) Pers culture medium for cultivating material, including following raw material: 50kg wood flour, 20kg Testa Tritici,
15kg bagasse, 15kg wormcast, 6kg distiller grains, 10kg straw, 10kg fertile soil, 2kg potassium dihydrogen phosphate, 1kg Gypsum Fibrosum.
This culture medium for cultivating material is made by following steps:
1) by crushed stalk, then according to all raw materials are mixed by ratio, in the environment of 40 DEG C, fermentation 3 days is stacked；
2) raw material after fermentation is loaded in Polypropylene Bag, and on Polypropylene Bag, open multiple osculum, be placed on autoclave
Middle sterilizing, preserves more than 4h at normal pressure 100 DEG C, taking-up is cooled to room temperature and makes bar-shaped culture medium for cultivating, culture medium for cultivating
Diameter is less than 10cm, and length is less than 50cm.
Embodiment three
Present embodiments provide a kind of Morchella esculenta (L.) Pers culture medium for cultivating material, including following raw material: 40kg wood flour, 15kg Testa Tritici,
10kg bagasse, 10kg wormcast, 4kg distiller grains, 13kg straw, 15kg fertile soil, 4kg potassium dihydrogen phosphate, 2kg Gypsum Fibrosum.
This culture medium for cultivating material is made by following steps:
1) by crushed stalk, then according to all raw materials are mixed by ratio, in the environment of 25～40 DEG C stack fermentation 3～
6 days；
2) raw material after fermentation is loaded in Polypropylene Bag, and on Polypropylene Bag, open multiple osculum, be placed on autoclave
Middle sterilizing, preserves more than 4h at normal pressure 100 DEG C, taking-up is cooled to room temperature and makes bar-shaped culture medium for cultivating, culture medium for cultivating
Diameter is less than 10cm, and length is less than 50cm.
Embodiment four
Present embodiments provide a kind of Morchella esculenta (L.) Pers culture medium for cultivating material, including following raw material: 50kg wood flour, 20kg Testa Tritici,
13kg bagasse, 15kg wormcast, 6kg distiller grains, 13kg straw, 12kg fertile soil, 2kg potassium dihydrogen phosphate, 1kg Gypsum Fibrosum.
This culture medium for cultivating material is made by following steps:
1) by crushed stalk, then according to all raw materials are mixed by ratio, in the environment of 40 DEG C, fermentation 3 days is stacked；
2) raw material after fermentation is loaded in Polypropylene Bag, and on Polypropylene Bag, open multiple osculum, be placed on autoclave
Middle sterilizing, preserves more than 4h at normal pressure 100 DEG C, taking-up is cooled to room temperature and makes bar-shaped culture medium for cultivating, culture medium for cultivating
Diameter is less than 10cm, and length is less than 50cm.
Embodiment five
Present embodiments provide a kind of Morchella esculenta (L.) Pers culture medium for cultivating material, including following raw material: 45kg wood flour, 18kg Testa Tritici,
12kg bagasse, 13kg wormcast, 5kg distiller grains, 12kg straw, 13kg fertile soil, 3kg potassium dihydrogen phosphate, 1.5kg Gypsum Fibrosum.
This culture medium for cultivating material is made by following steps:
1) by crushed stalk, then according to all raw materials are mixed by ratio, in the environment of 30 DEG C, fermentation 4 days is stacked；
2) raw material after fermentation is loaded in Polypropylene Bag, and on Polypropylene Bag, open multiple osculum, be placed on autoclave
Middle sterilizing, preserves more than 4h at normal pressure 100 DEG C, taking-up is cooled to room temperature and makes bar-shaped culture medium for cultivating, culture medium for cultivating
Diameter is less than 10cm, and length is less than 50cm.
In order to prove the technique effect of the present invention, present invention also offers following experimental example.
Experimental group 1～5
Experimental group 1～5 uses the plantation culture base-material inoculation of embodiment one～five through activating respectively, the sheep of amplification culture
Tripe thalline (often group 100 plantation culture base-material, uses the Gaster caprae seu Ovis thalline with batch, and the inoculum concentration often organized is identical), then
Carry out indoor cultivation, indoor are carried out disinfection, first at one block of every layer of bed surface upper berth plastic sheeting, then thick for paving 3cm fertile soil,
Being arranged one by one in bed by the bacterium rod of the plantation culture base-material of inoculation after clapping, the drained bacterium rear water of rod can earthing 3-again
5cm, surface is covered broad leaf tree fallen leaves thick for 2cm again, is kept ground moistening, air humidity 85%-95%；Cultivation temperature is 10～14
DEG C, after 30 days, there is the bacterium rod quantity of Morchella esculenta (L.) Pers sporophore in statistics；And pluck Morchella esculenta (L.) Pers, weigh and calculate average product.
Matched group 1:
Cultivar culture medium disclosed in patent documentation 1 is used (in terms of mass fraction, to take after Semen Tritici aestivi 75% boils and pull out, put
Enter 15% dowel, 5% Testa Tritici and 5% earth to stir, water content 60%-80%, load in Polypropylene Bag and seal, by described
Polypropylene Bag is placed on sterilizing in autoclave, preserves 4 hours at normal pressure 100 DEG C, takes out and is cooled to room temperature) inoculation activates, expansion training
The Gaster caprae seu Ovis thalline supported (100 Cultivar culture medium, use the Gaster caprae seu Ovis thalline identical with experimental group, and inoculum concentration and experimental group
Identical)；The same experimental group of other conditions, after 30 days, there is the bacterium rod quantity of Morchella esculenta (L.) Pers sporophore in statistics；And pluck Morchella esculenta (L.) Pers,
Weigh and calculate average product.
Through statistics, the success rate of experimental group 1～5 (and growing the bacterium rod of Morchella esculenta (L.) Pers sporophore) is 91%, 92%,
90%, 94%, 94%；And the success rate of matched group 1 is 76%；The average product higher than matched group 1 51 of experimental group 1～5～
58%.
Claims (4)
1. a Morchella esculenta (L.) Pers culture medium for cultivating material, it is characterised in that: include the raw material of following weight portion: 30～50 parts of wood flours, 10
～20 parts of Testa Tritici, 10～15 parts of bagasse, 5～15 parts of wormcasts, 3～6 parts of distiller grains, 10～15 parts of straws, 10～15 parts of humics
Soil, 2～4 parts of potassium dihydrogen phosphates, 1～2 part of Gypsum Fibrosum.
2. Morchella esculenta (L.) Pers culture medium for cultivating material as claimed in claim 1, it is characterised in that: include the raw material of following weight portion: 40
～50 parts of wood flours, 15～20 parts of Testa Tritici, 10～13 parts of bagasse, 10～15 parts of wormcasts, 4～6 parts of distiller grains, 10～13 parts of straw
Stalk, 12～15 parts of fertile soil, 2～4 parts of potassium dihydrogen phosphates, 1～2 part of Gypsum Fibrosum.
3. Morchella esculenta (L.) Pers culture medium for cultivating material as claimed in claim 1, it is characterised in that: include the raw material of following weight portion: 45
Part wood flour, 18 parts of Testa Tritici, 12 parts of bagasse, 13 parts of wormcasts, 5 parts of distiller grains, 12 parts of straws, 13 parts of fertile soil, 3 parts of biphosphates
Potassium, 1.5 parts of Gypsum Fibrosum.
4. the Morchella esculenta (L.) Pers culture medium for cultivating material as described in claims 1 to 3 any one, it is characterised in that: this culture medium for cultivating
Material is made by following steps:
1) by crushed stalk, then according to all raw materials are mixed by ratio, in the environment of 25～40 DEG C, fermentation 3～6 days is stacked；
2) raw material after fermentation is loaded in Polypropylene Bag, and on Polypropylene Bag, open multiple osculum, be placed in autoclave and go out
Bacterium, preserves more than 4h at normal pressure 100 DEG C, taking-up is cooled to room temperature and makes bar-shaped culture medium for cultivating.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610424635.3A CN106116743A (en) | 2016-06-15 | 2016-06-15 | A kind of Morchella esculenta (L.) Pers culture medium for cultivating material |
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| CN201610424635.3A CN106116743A (en) | 2016-06-15 | 2016-06-15 | A kind of Morchella esculenta (L.) Pers culture medium for cultivating material |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866223A (en) * | 2017-01-10 | 2017-06-20 | 徐州鸿宇农业科技有限公司 | A kind of edible fungus culture medium |
| CN107567954A (en) * | 2017-10-13 | 2018-01-12 | 太湖县金江源农业发展有限公司 | A kind of cultural method of hickory chick |
| CN108586126A (en) * | 2018-04-27 | 2018-09-28 | 四川省林业科学研究院 | Hickory chick cultivating soil modifying agent and its preparation method and application |
| CN108887082A (en) * | 2018-07-25 | 2018-11-27 | 贵州晏山农业发展有限公司 | A kind of cultural method improving hickory chick yield |
| CN111357567A (en) * | 2020-03-18 | 2020-07-03 | 务川自治县安博农业科技发展有限公司 | Nutrient bar and method for planting morchella esculenta by using same |
| CN112931052A (en) * | 2021-04-02 | 2021-06-11 | 甘肃省科学院生物研究所 | Morchella cultivation medium and preparation method thereof |
| CN113597975A (en) * | 2021-09-01 | 2021-11-05 | 绵阳市经科菌业有限责任公司 | Preparation method of high-yield culture medium for morchella cultivars |
| CN114342739A (en) * | 2022-01-28 | 2022-04-15 | 杨凌职业技术学院 | Morchella cultivation medium and cultivation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866223A (en) * | 2017-01-10 | 2017-06-20 | 徐州鸿宇农业科技有限公司 | A kind of edible fungus culture medium |
| CN107567954A (en) * | 2017-10-13 | 2018-01-12 | 太湖县金江源农业发展有限公司 | A kind of cultural method of hickory chick |
| CN108586126A (en) * | 2018-04-27 | 2018-09-28 | 四川省林业科学研究院 | Hickory chick cultivating soil modifying agent and its preparation method and application |
| CN108887082A (en) * | 2018-07-25 | 2018-11-27 | 贵州晏山农业发展有限公司 | A kind of cultural method improving hickory chick yield |
| CN111357567A (en) * | 2020-03-18 | 2020-07-03 | 务川自治县安博农业科技发展有限公司 | Nutrient bar and method for planting morchella esculenta by using same |
| CN112931052A (en) * | 2021-04-02 | 2021-06-11 | 甘肃省科学院生物研究所 | Morchella cultivation medium and preparation method thereof |
| CN113597975A (en) * | 2021-09-01 | 2021-11-05 | 绵阳市经科菌业有限责任公司 | Preparation method of high-yield culture medium for morchella cultivars |
| CN114342739A (en) * | 2022-01-28 | 2022-04-15 | 杨凌职业技术学院 | Morchella cultivation medium and cultivation method thereof |
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