CN109496690A - Hickory chick Primary spawn matrix, preparation method and Primary spawn method - Google Patents
Hickory chick Primary spawn matrix, preparation method and Primary spawn method Download PDFInfo
- Publication number
- CN109496690A CN109496690A CN201811435539.4A CN201811435539A CN109496690A CN 109496690 A CN109496690 A CN 109496690A CN 201811435539 A CN201811435539 A CN 201811435539A CN 109496690 A CN109496690 A CN 109496690A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of hickory chick Primary spawn matrix and is made based on parts by weight of following component: 1-5 parts of straw, 1-3 parts of lotus seed shell, 2-3 parts of rape straw, 0.1-0.3 parts of soil, 1-2 parts of nutritive water.It can be used for cultivating the high hickory chick original seed of exocellular polysaccharide content uniform in size.
Description
Technical field
The present invention relates to edible mushroom technical field, especially a kind of hickory chick Primary spawn matrix, preparation method and original seed
Cultural method.
Background technique
Hickory chick is a kind of Rare edible fungus kind, is gained the name because of its cap surface irregularity, shape such as sheep tripe, You Mingcao
Large bamboo hat with a conical crown and broad brim bamboo is edible mushroom and the medicinal fungus of a kind of preciousness.Hickory chick is breathed with cough for stagnation of QI due to dyspepsia, abdominal fullness and distention, phlegm stops up the circulation of vital energy in the wrong direction.It is tied
Structure is similar to cup fungi, and top is netted in fold, both as a honeycomb, also like a sheep tripe, thus gains the name.
Hickory chick artificial cultivation is currently mainly based on the Yunnan of Sichuan, and formulation composition is all with the leftover bits and pieces of local crops
Based on, at low cost, materials are convenient, and the original seed usage amount of hickory chick is big, if drawn materials from other places, transportation cost is substantially and material
Cost maintains an equal level, and cultivation cost is significantly increased, and increases operations risks.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art to provide a kind of hickory chick Primary spawn matrix, preparation method
And Primary spawn method, it can be used for cultivating high-quality hickory chick original seed.
Hickory chick Primary spawn matrix of the present invention is made of following component based on parts by weight:
1-5 parts of straw, 1-3 parts of lotus seed shell, 2-3 parts of rape straw, 0.1-0.3 parts of soil, 1-2 parts of nutritive water.
The preferred embodiment of the hickory chick Primary spawn matrix is made of following component based on parts by weight:
1 part of straw, 1 part of lotus seed shell, 2 parts of rape straw, 0.2 part of soil, 3 parts of nutritive water.
The nutritive water based on parts by weight, is made of following component:
0.02-0.03 parts of dipotassium hydrogen phosphate, carrot extract 10-20ppm, chlorogenic acid 2-4ppm, vitamin C 10-15ppm,
10 parts of water.
Hickory chick Primary spawn matrix of the present invention the preparation method is as follows:
(1) lotus seed shell is broken into 1-2cm size, rape straw is cut into 1-2cm size, then ferments;
(2) finally by straw dissection at 1-2cm size, lotus seed shell, rape straw, soil and the nutritive water that fermentation is added are stirred and evenly mixed
?.
Wherein the specific fermentation process of step (1) is as follows:
(a) feed fermentation bacterium solution and brown sugar are diluted with water, obtain fermentation mixed liquor, the weight of feed fermentation bacterium solution, brown sugar and water
Amount is than being 0.5-1:1-1.5:10-15;
(b) lotus seed shell and rape straw being uniformly mixed, obtains fermentation materials, added water and stirred uniformly, water content is controlled in 60-70%,
Judgment criteria are as follows: grab a material with hand-tight, webs water breakthrough does not drip;
(c) fermentation mixed liquor is uniformly sprayed on material with sprayer, is overturned while spraying, is allowed to uniform;Fermentation
The weight ratio of mixed liquor and fermentation materials is 1:60-100;
(d) the above-mentioned material mixed thoroughly is piled into height in 40-50cm, length and width arbitrarily material heap plugs thermometer, covers heat preservation
Moisturizing material makes its fermentation;
(e) it is overturned when temperature reaches 35-40 DEG C once, temperature is more than 45 DEG C of degree, and it is rotten to be easy excessive fermentation;Entirely fermented
Journey overturns 1-2 time, and when overflowing with wine aroma, showing to ferment is basically completed, and should remove covering at this time, and scattered heap is made thinner drop
Temperature heats up once again to prevent secondary fermentation.
The cultural method of original seed of the present invention is as follows:
(1) level-one kind is bought;
(2) after sterilization and cooling by pedigree seed culture medium pack, sterile working accesses hickory chick, and constant temperature is trained at a temperature of 23 DEG C -27 DEG C
Support to obtain hickory chick original seed.
Beneficial effects of the present invention are as follows:
Hickory chick Primary spawn matrix of the present invention, it is at low cost, and the hickory chick original seed of high quality can be cultivated.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.
The formula of nutritive water is as follows in embodiment:
Dipotassium hydrogen phosphate 0.03kg, carrot extract 10ppm, chlorogenic acid 3ppm, vitamin C 10ppm, water 10kg.
Embodiment, comparative example culture substrate the preparation method is as follows:
(1) lotus seed shell is broken into 1-2cm size, rape straw is cut into 1-2cm size, then ferments;
(2) finally by straw dissection at 1-2cm size, lotus seed shell, rape straw, soil and the nutritive water that fermentation is added are stirred and evenly mixed
?.
Wherein the specific fermentation process of step (1) is as follows:
(a) feed fermentation bacterium solution and brown sugar are diluted with water, obtain fermentation mixed liquor, the weight of feed fermentation bacterium solution, brown sugar and water
Amount is than being 0.5:1:15;
(b) lotus seed shell and rape straw being uniformly mixed, obtains fermentation materials, added water and stirred uniformly, water content is controlled in 60-70%,
Judgment criteria are as follows: grab a material with hand-tight, webs water breakthrough does not drip;
(c) fermentation mixed liquor is uniformly sprayed on material with sprayer, is overturned while spraying, is allowed to uniform;Fermentation
The weight ratio of mixed liquor and fermentation materials is 1:70;
(d) the above-mentioned material mixed thoroughly is piled into height at 40-50 centimetres, length and width arbitrarily material heap plugs thermometer, covers guarantor
Warm moisturizing material, makes its fermentation;
(e) it is overturned once when temperature reaches 35 DEG C, temperature is more than 45 DEG C, and it is rotten to be easy excessive fermentation;Entire fermentation process is turned over
1-2 times, when being overflowed with wine aroma, showing that fermentation is basically completed, should remove covering at this time, scattered heap is made thinner cooling, with
Anti- secondary fermentation heats up once again.
Embodiment 1
The cultural method of hickory chick original seed:
(1) preparation of level-one kind: hickory chick (Morchellasp.) No. 801 purchases are in Mianyang, Sichuan edible mushroom research institute;
(2) Primary spawn matrix forms: straw 1kg, lotus seed shell 1kg, rape straw 2kg, soil 0.2kg, nutritive water 1kg;
(3) Primary spawn matrix is fitted into edible mushroom polypropylene plastics pocket, it is elastic to be suitable for, it is sealed with cotton plug, high pressure sterilization;
After cooling, sterile working accesses hickory chick level-one kind, can obtain Submerged Culture of Morchella conica original seed within constant temperature incubation 17 days at 25 DEG C.
Embodiment 2
The cultural method of hickory chick original seed:
(1) preparation of level-one kind: hickory chick (Morchellasp.) No. 801 purchases are in Mianyang, Sichuan edible mushroom research institute;
(2) Primary spawn matrix forms: straw 2kg, lotus seed shell 1kg, rape straw 2kg, soil 0.3kg, nutritive water 1.2kg;
(3) Primary spawn matrix is fitted into edible mushroom polypropylene plastics pocket, it is elastic to be suitable for, it is sealed with cotton plug, high pressure sterilization;
After cooling, sterile working accesses hickory chick level-one kind, can obtain Submerged Culture of Morchella conica original seed within constant temperature incubation 16 days at 25 DEG C.
Embodiment 3
The cultural method of hickory chick original seed:
(1) preparation of level-one kind: hickory chick (Morchellasp.) No. 801 purchases are in Mianyang, Sichuan edible mushroom research institute;
(2) Primary spawn matrix forms: straw 1kg, lotus seed shell 1.2kg, rape straw 2.5kg, soil 0.2kg, nutritive water 1kg;
(3) Primary spawn matrix is fitted into edible mushroom polypropylene plastics pocket, it is elastic to be suitable for, it is sealed with cotton plug, high pressure sterilization;
After cooling, sterile working accesses hickory chick level-one kind, can obtain Submerged Culture of Morchella conica original seed within constant temperature incubation 17 days at 25 DEG C.
Embodiment 4
(1) preparation of level-one kind: hickory chick (Morchellasp.) No. 801 purchases are in Mianyang, Sichuan edible mushroom research institute;
(2) Primary spawn matrix forms: straw 1kg, lotus seed shell 1.5kg, rape straw 2.2kg, soil 0.3kg, nutritive water
1.2kg;
(3) Primary spawn matrix is fitted into edible mushroom polypropylene plastics pocket, it is elastic to be suitable for, it is sealed with cotton plug, high pressure sterilization;
After cooling, sterile working accesses hickory chick level-one kind, can obtain Submerged Culture of Morchella conica original seed within constant temperature incubation 17 days at 25 DEG C.
Comparative example 1
(1) preparation of level-one kind: hickory chick (Morchellasp.) No. 801 purchases are in Mianyang, Sichuan edible mushroom research institute;
(2) Primary spawn matrix forms: straw 1kg, lotus seed shell 1.5kg, rape straw 2.2kg, soil 0.3kg, nutritive water
1.2kg, wherein nutritive water is free of chlorogenic acid, formula are as follows: dipotassium hydrogen phosphate 0.03kg, carrot extract 10ppm, vitamin
C13ppm, water 10kg;
(3) Primary spawn matrix is fitted into edible mushroom polypropylene plastics pocket, it is elastic to be suitable for, it is sealed with cotton plug, high pressure sterilization;
After cooling, sterile working accesses hickory chick level-one kind, can obtain Submerged Culture of Morchella conica original seed within constant temperature incubation 17 days at 25 DEG C.
Each embodiment, the quality of comparative example original seed comparison such as table 1.
Table 1
。
As it can be seen from table 1 the original seed that hickory chick culture substrate of the present invention obtains has the following advantages than comparative example: size is more
Uniformly, exocellular polysaccharide content improves.
The above content describes basic principles and main features of the invention, and the present invention is not limited to the above embodiments,
Without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements are all
It falls into scope of the claimed invention.
Claims (6)
1. a kind of hickory chick Primary spawn matrix, it is characterised in that based on parts by weight, be made of following component: 1-5 parts of straw,
1-3 parts of lotus seed shell, 2-3 parts of rape straw, 0.1-0.3 parts of soil, 1-2 parts of nutritive water.
2. hickory chick Primary spawn matrix according to claim 1, it is characterised in that based on parts by weight, by following component
It is made: 1 part of straw, 1 part of lotus seed shell, 2 parts of rape straw, 0.2 part of soil, 3 parts of nutritive water.
3. hickory chick Primary spawn matrix according to claim 1, it is characterised in that the nutritive water is with parts by weight
Meter, is made of following component: 0.02-0.03 parts of dipotassium hydrogen phosphate, carrot extract 10-20ppm, chlorogenic acid 2-4ppm, dimension
Raw element C10-15ppm, 10 parts of water.
4. the preparation method of hickory chick Primary spawn matrix according to claim 1, it is characterised in that successively include following step
It is rapid:
(1) lotus seed shell is broken into 1-2cm size, rape straw is cut into 1-2cm size, then ferments;
(2) finally by straw dissection at 1-2cm size, lotus seed shell, rape straw, soil and the nutritive water that fermentation is added are stirred and evenly mixed
?.
5. the preparation method of hickory chick Primary spawn matrix according to claim 4, it is characterised in that the specific hair of step (1)
Fermenting process is as follows:
(a) feed fermentation bacterium solution and brown sugar are diluted with water, obtain fermentation mixed liquor, the weight of feed fermentation bacterium solution, brown sugar and water
Amount is than being 0.5-1:1-1.5:10-15;
(b) lotus seed shell and rape straw are uniformly mixed, obtain fermentation materials, added water and stirred uniformly, water content is controlled in 60-70%;
(c) fermentation mixed liquor is uniformly sprayed on material with sprayer, is overturned while spraying, is allowed to uniform;Fermentation
The weight ratio of mixed liquor and fermentation materials is 1:60-100;
(d) the above-mentioned material mixed thoroughly is piled into height in 40-50cm, length and width arbitrarily material heap plugs thermometer, covers heat preservation
Moisturizing material makes its fermentation;
(e) it being overturned when temperature reaches 35-40 DEG C once, entire fermentation process overturns 1-2 times, when being overflowed with wine aroma,
Show that fermentation is basically completed, remove covering at this time, scattered heap is made thinner cooling.
6. a kind of hickory chick Primary spawn method, it is characterised in that successively include following not step:
(1) level-one kind is bought;
(2) after sterilization and cooling by pedigree seed culture medium pack, sterile working accesses hickory chick, and constant temperature is trained at a temperature of 23 DEG C -27 DEG C
Support to obtain hickory chick original seed.
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Cited By (1)
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CN113597975A (en) * | 2021-09-01 | 2021-11-05 | 绵阳市经科菌业有限责任公司 | Preparation method of high-yield culture medium for morchella cultivars |
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Application publication date: 20190322 |