CN103694016A - Ganoderma lucidum culture medium and culture method thereof - Google Patents
Ganoderma lucidum culture medium and culture method thereof Download PDFInfo
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- CN103694016A CN103694016A CN201310651276.1A CN201310651276A CN103694016A CN 103694016 A CN103694016 A CN 103694016A CN 201310651276 A CN201310651276 A CN 201310651276A CN 103694016 A CN103694016 A CN 103694016A
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- glossy ganoderma
- ganoderma lucidum
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- ramulus mori
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Abstract
The invention relates to a ganoderma lucidum culture medium which comprises the following components in percentage by mass: 50-60% of mulberry twig, 20-30% of cottonseed hull, 10-20% of ganoderma lucidum slag, 5-10% of bran, 1-5% of corn flour and 1-3% of calcium carbonate. The ganoderma lucidum culture method comprises the following steps: (1) material preparation: airing the mulberry twig, and pulverizing into wood dust; (2) proportioning: mixing the mulberry twig wood dust, cottonseed hull, ganoderma lucidum slag, bran, corn flour and calcium carbonate, adding water which is 1-1.5 times by mass of the culture medium, and uniformly mixing; (3) fermentation: piling the mixed culture medium, and fermenting at normal temperature for 5-10 hours to obtain the culture medium; (4) bagging: adding the culture medium into a bacterium cylinder bag to obtain a culture bacterium cylinder, sterilizing, cooling, and inoculating the ganoderma lucidum strain; (5) culturing for 30-35 days to grow the ganoderma lucidum; and (6) after 55-60 days, carrying out mushroom emergence management to obtain the mature ganoderma lucidum sporophore. By using the low-cost mulberry to culture the ganoderma lucidum, the cultured ganoderma lucidum has high nutrient content, and the production cost is lowered.
Description
Technical field
The present invention relates to cultivation matrix and the cultivating method thereof of a kind of edible mushrooms, relate in particular to cultivation matrix and the cultivating method thereof of a kind of glossy ganoderma.
Background technology
Glossy ganoderma belongs to Basidiomycetes, Aphyllophorales, polyporaceae, Ganoderma.Glossy ganoderma is just considered to lucky, valuable, fine, long-lived symbol since ancient times, the title that from ancient times just has celestial grass, refreshing grass, auspicious grass, representing that auspicious sign, longevity, good fortune are expensive, pharmacological research shows, glossy ganoderma also have protect the liver, removing toxic substances, cardiac stimulant, calm the nerves, the function such as beneficial lung qi, sharp joint, the direct-view for a long time of chinese tradition medical science for strengthening by means of tonics, consolidate the precious herbal medicine of righting.Current glossy ganoderma artificial culture mainly contains cultivation basswood and cultivating in bag mode.Cultivation basswood is traditional Ganoderma Lucidum method, utilize glossy ganoderma to grow in blue or green steel wood and wait the feature on timber, the mode that adopts intercept-pack-sterilizing-inoculation-mycelia management-earthing to go out sesame is cultivated, its glossy ganoderma strain shape is good, quality is good, but need to consume a large amount of forest tree resources, and take a large amount of land resources.Pocket type cultivation is the cultivating method of development in recent years, will pack into and cultivate in bag according to the substratum of the material formulation of glossy ganoderma growth desired nutritional element, under the temperature condition adapting with Ganderma lucidum strain habit, condition, in indoor bacterium, cultivate and go out sesame.But along with making the development of plate, paper-making industry, timber resources demand increases, can cause raising for traditional broad-leaved timber cost of cultivating ganoderma; In addition, the seeds of Song Shan mono-class, it is wooden contains the material that perfume oil, resin etc. are unfavorable for glossy ganoderma growth, causes the wood resources acquisition approach that can be used in cultivating ganoderma to become narrow and small and nervous.
Summary of the invention
For above-mentioned defect, the technical problem that the present invention solves with a kind of cultivation of glossy ganoderma matrix is provided, adopt Ramulus Mori and glossy ganoderma slag for cultivating glossy ganoderma, another object of the present invention is to provide a kind of glossy ganoderma growth cycle ganoderma lucidum cultivation method fast, with low cost.
For solving above technical problem, the present invention has adopted following technical scheme:
A cultivation matrix for glossy ganoderma, cultivation matrix calculates and comprises following component by quality percentage composition: 50～60% ramulus mori, 20～30% cotton seed hulls, 10～20% glossy ganoderma slag, 5～10% wheat bran, 1～5% Semen Maydis powder, 1～3% calcium carbonate.
Preferably, cultivation matrix calculates and comprises following component by quality percentage composition: 53% ramulus mori, 23% cotton seed hulls, 11% glossy ganoderma slag, 7% wheat bran, 3% Semen Maydis powder, 3% calcium carbonate.
A cultivating method for glossy ganoderma, comprises the following steps:
(1) get the raw materials ready, ramulus mori is air-dry and be ground into wood chip;
(2) batching, is uniformly mixed mulberry wood chips, cotton seed hulls, glossy ganoderma slag, wheat bran, Semen Maydis powder and calcium carbonate, then adds the water of 1～1.5 times of cultivation matrix quality, and remix is even;
(3) fermentation, the cultivation matrix heap mixing is in heaps, and normal temperature bottom fermentation 5～10 hours, obtains cultivation matrix;
(4) pack, packs the cultivation matrix making in plastics bacterium cylinder bag into and makes cultivation bacterium cylinder, then carries out sterilising treatment, accesses after cooling Ganderma lucidum strain;
(5) cultivate after 30～35 days and go out sesame;
After (6) 55～60 days management of producing mushroom, obtain ripe Ganoderma sporophore.
Preferably, described in described step (1) by ramulus mori air-dry to its water content be 30～40% of ramulus mori dry weight.
Preferably, the cultivation in described step (5) is to be 20～25 ℃ in temperature, and relative air humidity is 55～65%.
Preferably, the plastics bacterium cylinder of described step (4) is the polypropylene strain bag of 15cm * 30cm or 17cm * 33cm.
Preferably, the management of producing mushroom temperature of described step (6) is 28～30 ℃, and relative air humidity is 85～90%.
Beneficial effect of the present invention, ganoderma lucidum cultivation method provided by the invention, uses mulberry cultivation glossy ganoderma, in the time of solving traditional cultivation glossy ganoderma, need to consume the problem of a large amount of timber, the mulberry tree that use cost is cheap carrys out cultivating ganoderma, and the ganoderma lucidum composition of cultivating is out high, and has reduced production cost.
Embodiment
Referring to embodiment, further describe the present invention, to make those skilled in the art can implement according to this with reference to specification sheets word, protection domain of the present invention is not limited by embodiments of the present invention.
Embodiment 1
A cultivation matrix for glossy ganoderma, cultivation matrix calculates and comprises following component by quality percentage composition: 50% ramulus mori, 30% cotton seed hulls, 10% glossy ganoderma slag, 5% wheat bran, 3% Semen Maydis powder, 2% calcium carbonate.
A cultivating method for glossy ganoderma, comprises the following steps:
(1) get the raw materials ready, by ramulus mori air-dry to its water content be ramulus mori dry weight 40% after, and be ground into wood chip;
(2) batching, is uniformly mixed mulberry wood chips, cotton seed hulls, glossy ganoderma slag, wheat bran, Semen Maydis powder and calcium carbonate, then adds the water of 1 times of cultivation matrix quality, and remix is even;
(3) fermentation, the cultivation matrix heap mixing is in heaps, and normal temperature bottom fermentation 5 hours, obtains cultivation matrix;
(4) pack, packs the cultivation matrix making in the polypropylene strain bag of 15cm * 30cm and makes cultivation bacterium cylinder, then carries out sterilising treatment, accesses after cooling Ganderma lucidum strain;
(5) in temperature, be 20 ℃, under the condition that relative air humidity is 55%, cultivate and go out sesame after 30 days;
(6) in temperature, be 30 ℃, under the condition that relative air humidity is 85%, after 55 days management of producing mushroom, obtain ripe Ganoderma sporophore.
Embodiment 2
A cultivation matrix for glossy ganoderma, cultivation matrix calculates and comprises following component by quality percentage composition: 60% ramulus mori, 20% cotton seed hulls, 10% glossy ganoderma slag, 6% wheat bran, 3% Semen Maydis powder, 1% calcium carbonate.
A cultivating method for glossy ganoderma, comprises the following steps:
(1) get the raw materials ready, by ramulus mori air-dry to its water content be ramulus mori dry weight 30% after, and be ground into wood chip;
(2) batching, is uniformly mixed mulberry wood chips, cotton seed hulls, glossy ganoderma slag, wheat bran, Semen Maydis powder and calcium carbonate, then adds the water of 1.5 times of cultivation matrix quality, and remix is even;
(3) fermentation, the cultivation matrix heap mixing is in heaps, and normal temperature bottom fermentation 10 hours, obtains cultivation matrix;
(4) pack, packs the cultivation matrix making in the polypropylene strain bag of 15cm * 30cm and makes cultivation bacterium cylinder, then carries out sterilising treatment, accesses after cooling Ganderma lucidum strain;
(5) in temperature, be 25 ℃, under the condition that relative air humidity is 65%, cultivate and go out sesame after 35 days;
(6) in temperature, be 28 ℃, under the condition that relative air humidity is 85%, after 55 days management of producing mushroom, obtain ripe Ganoderma sporophore.
Embodiment 3
A cultivation matrix for glossy ganoderma, cultivation matrix calculates and comprises following component by quality percentage composition: 53% ramulus mori, 23% cotton seed hulls, 11% glossy ganoderma slag, 7% wheat bran, 3% Semen Maydis powder, 3% calcium carbonate.
A cultivating method for glossy ganoderma, comprises the following steps:
(1) get the raw materials ready, by ramulus mori air-dry to its water content be ramulus mori dry weight 35% after, and be ground into wood chip;
(2) batching, is uniformly mixed mulberry wood chips, cotton seed hulls, glossy ganoderma slag, wheat bran, Semen Maydis powder and calcium carbonate, then adds the water of 1.3 times of cultivation matrix quality, and remix is even;
(3) fermentation, the cultivation matrix heap mixing is in heaps, and normal temperature bottom fermentation 7 hours, obtains cultivation matrix;
(4) pack, packs the cultivation matrix making in the polypropylene strain bag of 17cm * 33cm and makes cultivation bacterium cylinder, then carries out sterilising treatment, accesses after cooling Ganderma lucidum strain;
(5) in temperature, be 23 ℃, under the condition that relative air humidity is 60%, cultivate and go out sesame after 33 days;
(6) in temperature, be 29 ℃, under the condition that relative air humidity is 87%, after 58 days management of producing mushroom, obtain ripe Ganoderma sporophore.
Claims (7)
1. the cultivation matrix of a glossy ganoderma, it is characterized in that, cultivation matrix calculates and comprises following component by quality percentage composition: 50～60% ramulus mori, 20～30% cotton seed hulls, 10～20% glossy ganoderma slag, 5～10% wheat bran, 1～5% Semen Maydis powder, 1～3% calcium carbonate.
2. cultivation of glossy ganoderma matrix according to claim 1, is characterized in that, cultivation matrix calculates and comprises following component by quality percentage composition: 53% ramulus mori, 23% cotton seed hulls, 11% glossy ganoderma slag, 7% wheat bran, 3% Semen Maydis powder, 3% calcium carbonate.
3. a cultivating method for glossy ganoderma, is characterized in that, comprises the following steps:
(1) get the raw materials ready, ramulus mori is air-dry and be ground into wood chip;
(2) batching, is uniformly mixed mulberry wood chips, cotton seed hulls, glossy ganoderma slag, wheat bran, Semen Maydis powder and calcium carbonate, then adds the water of 1～1.5 times of cultivation matrix quality, and remix is even;
(3) fermentation, the cultivation matrix heap mixing is in heaps, and normal temperature bottom fermentation 5～10 hours, obtains cultivation matrix;
(4) pack, packs the cultivation matrix making in bacterium cylinder bag into and makes cultivation bacterium cylinder, then carries out sterilising treatment, accesses after cooling Ganderma lucidum strain;
(5) cultivate after 30～35 days and go out sesame;
After (6) 55～60 days management of producing mushroom, obtain ripe Ganoderma sporophore.
4. the cultivating method of glossy ganoderma according to claim 3, is characterized in that, described in described step (1) by ramulus mori air-dry to its water content be 30～40% of ramulus mori dry weight.
5. the cultivating method of glossy ganoderma according to claim 3, is characterized in that, the cultivation in described step (5) is to be 20～25 ℃ in temperature, and relative air humidity is 55～65%.
6. the cultivating method of glossy ganoderma according to claim 3, is characterized in that, the plastics bacterium cylinder of described step (4) is the polypropylene strain bag of 15cm * 30cm or 17cm * 33cm.
7. the cultivating method of glossy ganoderma according to claim 3, is characterized in that, the management of producing mushroom temperature of described step (6) is 28～30 ℃, and relative air humidity is 85～90%.
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Cited By (9)
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| CN104072238A (en) * | 2014-06-27 | 2014-10-01 | 南京金湖食用菌专业合作社 | Ganoderma lucidum culture medium and preparation method thereof |
| CN104303845A (en) * | 2014-11-03 | 2015-01-28 | 河北大学 | Method for cultivating lucid ganoderma through thorns crumbs |
| CN104350953A (en) * | 2014-11-19 | 2015-02-18 | 李志芳 | Cultivation method for ganoderma lucidum |
| CN104429592A (en) * | 2014-11-07 | 2015-03-25 | 成都煜泉绿健科技有限公司 | Ganoderma lucidum culture medium and ganoderma lucidum cultivation method |
| CN104591908A (en) * | 2015-02-08 | 2015-05-06 | 张进 | Canoderma lucidum culture medium and preparation method thereof |
| CN104770206A (en) * | 2015-03-17 | 2015-07-15 | 邬金梅 | Lucid ganoderma culture method |
| CN105474994A (en) * | 2015-12-16 | 2016-04-13 | 黑龙江众生生物工程有限公司 | Original-ecology wild-cultivation-simulated method of ganodorma lucidum |
| CN107698317A (en) * | 2017-10-20 | 2018-02-16 | 贵州省印江县印兰生态菌业有限公司 | A kind of mushroom culture medium for improving lentinan content and preparation method thereof |
| CN109315221A (en) * | 2018-11-16 | 2019-02-12 | 四川岚晟生物科技有限公司 | Cultivation of glossy ganoderma matrix based on ganoderma lucidum slag and preparation method thereof, ganoderma lucidum cultivation method |
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Cited By (13)
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| CN104072238B (en) * | 2014-06-27 | 2016-08-17 | 南京金湖食用菌专业合作社 | A kind of Medium for Ganoderma lucidum and preparation method thereof |
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| CN104303845A (en) * | 2014-11-03 | 2015-01-28 | 河北大学 | Method for cultivating lucid ganoderma through thorns crumbs |
| CN104429592B (en) * | 2014-11-07 | 2016-12-07 | 成都煜泉绿健科技有限公司 | A kind of cultural method of Ganoderma |
| CN104429592A (en) * | 2014-11-07 | 2015-03-25 | 成都煜泉绿健科技有限公司 | Ganoderma lucidum culture medium and ganoderma lucidum cultivation method |
| CN104350953B (en) * | 2014-11-19 | 2016-08-31 | 李志芳 | A kind of breeding method of blood Ganoderma |
| CN104350953A (en) * | 2014-11-19 | 2015-02-18 | 李志芳 | Cultivation method for ganoderma lucidum |
| CN104591908A (en) * | 2015-02-08 | 2015-05-06 | 张进 | Canoderma lucidum culture medium and preparation method thereof |
| CN104591908B (en) * | 2015-02-08 | 2017-09-22 | 康道生物（南通）有限公司 | A kind of cultivation of glossy ganoderma matrix and preparation method thereof |
| CN104770206A (en) * | 2015-03-17 | 2015-07-15 | 邬金梅 | Lucid ganoderma culture method |
| CN105474994A (en) * | 2015-12-16 | 2016-04-13 | 黑龙江众生生物工程有限公司 | Original-ecology wild-cultivation-simulated method of ganodorma lucidum |
| CN107698317A (en) * | 2017-10-20 | 2018-02-16 | 贵州省印江县印兰生态菌业有限公司 | A kind of mushroom culture medium for improving lentinan content and preparation method thereof |
| CN109315221A (en) * | 2018-11-16 | 2019-02-12 | 四川岚晟生物科技有限公司 | Cultivation of glossy ganoderma matrix based on ganoderma lucidum slag and preparation method thereof, ganoderma lucidum cultivation method |
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Application publication date: 20140402 |