CN104429592B - A kind of cultural method of Ganoderma - Google Patents
A kind of cultural method of Ganoderma Download PDFInfo
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- CN104429592B CN104429592B CN201410626258.2A CN201410626258A CN104429592B CN 104429592 B CN104429592 B CN 104429592B CN 201410626258 A CN201410626258 A CN 201410626258A CN 104429592 B CN104429592 B CN 104429592B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention belongs to Chinese crude drug technical field, the concrete cultural method that a kind of Ganoderma is provided.The method comprises the steps: to weigh compost cotton seed hulls 50 300 weight portion, Ramulus Mori 10 80 weight portion, Rhizoma Smilacis Glabrae 8 50 weight portion, Bombyx mori L. 8 50 weight portion, Spica Prunellae 5 30 weight portion, Herba Commelinae 5 30 weight portion by weight；Pulverize after cotton seed hulls, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L., Spica Prunellae and Herba Commelinae are mixed, after adding distilled water stirring, measure the water content in compost, water content control between 50 65%, then vexed 6 15h of heap；Compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, seal bag mouth the most again and be prepared as compost storage bag and preserve 24 72h；Compost in storage bag is loaded polypropylene kind pocket, then carries out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；By kind of pocket traverse in fungus shelf, indoor temperature controls between 25 35 DEG C, and relative humidity controls between 75 90%, and indoor holding is dark；When Ganoderma cap launches, and cap starts keratin, starts to eject spore, can gather by bagging.It is fast that the Ganoderma that the inventive method is cultivated has a bacterium speed, and insect pest disease is few, and nutrient accumulation is many, with short production cycle, produces more stable advantage.
Description
Technical field
The invention belongs to technical field of medicine cultivation, be specifically related to the cultural method of a kind of Ganoderma.
Background technology
Ganoderma is Basidiomycetes Polyporaceae Ganoderma fungus, and modern medicine study shows, the pharmaceutical component of Ganoderma is the abundantest, wherein active ingredient
Including ganoderan, Ganoderma polypeptide, triterpenes, aminoacid, protein, steroid, mannitol, coumarin glycosides, alkaloid, organic acid, (master contains
Fumaric acid), and various trace elements etc..Ganoderma has dual regulation to human body, kind of being cured the disease, and relates to cardiovascular and cerebrovascular vessel, digestion, god
Warp, endocrine, breathe, each system such as motion, especially the most notable to the preventive and therapeutic effect of tumor, hepatic lesions, insomnia and aging.To this,
Ganoderma is widely used in the treatment of clinical disease.
At present, commercial type be divided into wild Ganoderma and artificial culture Ganoderma.Wild Ganoderma is born on base portion or the rotten wood of broad leaf tree, the corruption of trees
On rotten scar, the most also it is born on coniferous tree.Along with the medical value of Ganoderma is extensively recognized by people, the wild Ganoderma resource of remote, thickly forested mountains is also now
Constantly reducing, although annual Ganoderma reproductive capacity is the most weak, but the most seed ejection, also immaturity are not just being plucked by people, thus wild
Creature's sesame resource is falling sharply now year by year.For ensureing the normal medicine resource supply of Ganoderma, people's laggard row artificial culture of wild to Ganoderma training.
At present, Ganoderma artificial culture has segment wood cultivated and cultivating in bag, the segment wood cultivated method referring to use the preferential species of tree section of being cut into cultivating ganoderma.Section wood is planted
Training common are long section wood cultivation in raw material, short section of wooden cultivation in raw material, short section of wooden grog cultivation, stub cultivation and branch bundle cultivation etc..Cultivating in bag
Although ratio life cultivation complex procedures, power consumption is big, demands strict technology, but cultivating in bag has a bacterium speed soon, and mycelia is distribution area in Duan Mu
Extensively, nutrient accumulation is many, with short production cycle, produces relatively stable and easily obtains the advantages such as good quality and high output.But existing above two Ganoderma artificial culture
There is parasite or other pathogenic bacteria due to the substrate of Ganoderma in method, causes the Ganderma lucidum strain after transplanting to infect the most different classes of disease, and its miscellaneous bacteria is dirty
Contaminating the highest, cause Ganderma lucidum mycelia to be disintegrated, Nutrient medium wrecks, and time serious, substrate is gone mouldy smelly, and then affects the finished product of its Ganoderma further
Yield.Therefore, need a kind of new to suppress Ganoderma substrate germ reproduction in industrialized great production, reduce Ganderma lucidum strain infect parasite or other
The Medium for Ganoderma lucidum of pathogenic bacteria and cultural method thereof.
Summary of the invention
There is parasite or other pathogenic bacteria for solving the substrate of prior art Ganoderma, cause the Ganderma lucidum strain after transplanting easily to infect different classes of disease
Technical problem.The present invention provides one can suppress Ganoderma substrate germ reproduction, reduces Ganderma lucidum strain and infects the Ganoderma cultivation of parasite or other pathogenic bacteria
Base and cultural method thereof.
It is an object of the invention to be achieved through the following technical solutions: the cultural method of a kind of Ganoderma, it comprises the following steps:
The preparation of S1: compost: weigh compost cotton seed hulls 50-300 weight portion, Ramulus Mori 10-80 weight portion, Rhizoma Smilacis Glabrae 8-50 weight by weight
Amount part, Bombyx mori L. 8-50 weight portion, Spica Prunellae 5-30 weight portion, Herba Commelinae 5-30 weight portion；
S2: pulverize, after adding distilled water stirring after cotton seed hulls, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L., Spica Prunellae and the Herba Commelinae mixing in S1 step
Measure the water content in compost, water content control at 50-65%, the then vexed 6-15h of heap；
After the vexed end of S3: heap, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, the most again
Seal bag mouth and be prepared as compost storage bag preservation 24-72h；
S4: the compost in S3 step storage bag is loaded polypropylene kind pocket, then carries out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene
Plant pocket；
S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, relative humidity control 75 exists
Between-90%, indoor holding is dark；
S6: when Ganoderma cap launches, and cap starts keratin, starts to eject spore, can gather by bagging.
Preferably, the compost in S1 step and weight proportion thereof are: cotton seed hulls 80-200 weight portion, Ramulus Mori 10-80 weight portion, Rhizoma Smilacis Glabrae 8-50
Weight portion, Bombyx mori L. 8-50 weight portion, Spica Prunellae 5-30 weight portion, Herba Commelinae 5-30 weight portion.
Preferably, the compost in S1 step and weight proportion thereof are: cotton seed hulls 100-150 weight portion, Ramulus Mori 30-60 weight portion, Rhizoma Smilacis Glabrae
12-40 weight portion, Bombyx mori L. 12-40 weight portion, Spica Prunellae 10-20 weight portion, Herba Commelinae 10-20 weight portion.
Preferably, the compost in S1 step and weight proportion thereof are: cotton seed hulls 120 weight portion, Ramulus Mori 50 weight portion, Rhizoma Smilacis Glabrae 20 weight portion,
Bombyx mori L. 20 weight portion, Spica Prunellae 15 weight portion, Herba Commelinae 15 weight portion.
Preferably, the water content in S2 step compost at 55-60%, the then vexed 8-12h of heap.
Preferably, after the vexed end of S3 step heap, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia
Gas, seals bag mouth the most again and is prepared as compost storage bag preservation 36-48h.
Preferably, postvaccinal kind of pocket traverse of S4 step is in fungus shelf, and indoor temperature controls between 28-32 DEG C, and relative humidity controls
Between 80-85%.
Ramulus Mori of the present invention is the twig of moraceae plants Mulberry, and micro-hardship, property are flat, return liver, lung meridian, has wind-damp dispelling, profit joint, row aqueous vapor
Effect；Rhizoma Smilacis Glabrae is the dry rhizome of dicotyledon medicine liliaceous plant smilacis glabra, sweet, light, flat, returns liver, stomach, spleen channel, has removing toxic substances
Dehumidifying, the effect in profit joint；Bombyx mori L. is the dry feces of Bombycidae insecticide bombyx mori larva, pungent, sweet, warm, returns spleen, stomach warp, and having dispels the wind removes
Wet, the effect of regulating the function of the stomach to resolve the turbidity, promoting blood circulation to remove obstruction in the collateral；Spica Prunellae is the herb of Labiatae Prunella, and bitter, acrid, cold are returned liver, gallbladder meridian, had and let out clearly
Liver-fire, mass dissipating and swelling eliminating, the effect of heat-clearing and toxic substances removing；Herba Commelinae is the dry aerial parts of Commelianaceae plant Herba Commelinae, sweet in the mouth, micro-hardship, cold in nature,
There is the effect of swelling diuretic, heat-clearing and toxic substances removing.Sterilization treatment of the present invention can be any in normal-pressure sterilization, autoclaving or low pressure sterilizing
A kind of method.Whether thorough sterilizing is also is the key of cultivating ganoderma success or failure, wants timely sterilizing, want between bag and bag during sterilizing code bag after installing kind of pocket
Leaving space, autoclaving to put clean cold air, in order to avoid causing the sterilizing of false pressure the most thorough.Normal-pressure sterilization can use low-pressure polyethylene, polyacrylic bag
Son, autoclaving needs to use high-pressure polypropylene sack, can pack by hand and machine can also be used to pack to improve efficiency.In addition to above-mentioned sterilizing methods,
Can also first pack after kind of pocket and compost each independent sterilizing, the temperature for compost pack is advisable when being cooled to below 30 DEG C again.
The invention has the beneficial effects as follows: Ganodermataceae Dook culture material novel formula of the present invention, composition is simple, can suppress the germ reproduction of Ganoderma substrate, Ke Yiyou
The Ganderma lucidum strain that reduces of effect infects parasite or the probability of other pathogenic bacteria, can improve Ganoderma production.The cultural method of Ganoderma of the present invention is simple,
The Ganoderma being applicable to the different cultivars such as Ganoderma lucidum (Leyss. Ex Fr.) Karst., Ganoderma sinense Zhao Xu et Zhang, Ganoderma capenseD.A.Reid is cultivated and cultivation, and it is fast that the Ganoderma that the inventive method is cultivated has a bacterium speed,
Insect pest disease is few, and nutrient accumulation is many, with short production cycle, produces more stable advantage.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated:
Embodiment 1:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion be: cotton seed hulls 50g, Ramulus Mori 10g, Rhizoma Smilacis Glabrae 8g, Bombyx mori L. 8g, Spica Prunellae
5g, Herba Commelinae 5g.
Embodiment 2:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion be: cotton seed hulls 300g, Ramulus Mori 80g, Rhizoma Smilacis Glabrae 50g, Bombyx mori L. 50g, summer
Hay 30g, Herba Commelinae 30g.
Embodiment 3:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion be: cotton seed hulls 80g, Ramulus Mori 30g, Rhizoma Smilacis Glabrae 12g, Bombyx mori L. 12g, summer are withered
Grass 10g, Herba Commelinae 10g.
Embodiment 4:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion be: cotton seed hulls 100g, Ramulus Mori 60g, Rhizoma Smilacis Glabrae 40g, Bombyx mori L. 40g, summer
Hay 20g, Herba Commelinae 20g.
Embodiment 5:
A kind of Ganodermataceae Dook culture material, the formula of its raw material and weight proportion be: cotton seed hulls 120g, Ramulus Mori 50g, Rhizoma Smilacis Glabrae 20g, Bombyx mori L. 20g, summer
Hay 15g, Herba Commelinae 15g.
Embodiment 6:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 50g, Ramulus Mori by weight
10g, Rhizoma Smilacis Glabrae 8g, Bombyx mori L. 8g, Spica Prunellae 5g, Herba Commelinae 5g；S2: the cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L., summer
Pulverizing after hay and Herba Commelinae mixing, measure the water content in compost after adding distilled water stirring, water content control is at 50-65%, and then heap is vexed
6-15h；After the vexed end of S3: heap, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, so
After seal again bag mouth be prepared as compost storage bag preserve 24-72h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket, so
After carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, room
Interior temperature controls between 25-35 DEG C, and relative humidity controls between 75-90%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap starts
Keratin, starts to eject spore, can gather by bagging.
Embodiment 7:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 300g, Ramulus Mori by weight
80g, Rhizoma Smilacis Glabrae 50g, Bombyx mori L. 50g, Spica Prunellae 30g, Herba Commelinae 30g；S2: cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L.,
Pulverizing after Spica Prunellae and Herba Commelinae mixing, measure the water content in compost after adding distilled water stirring, water content control is at 50-65%, and then heap is vexed
6-15h；After the vexed end of S3: heap, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, so
After seal again bag mouth be prepared as compost storage bag preserve 24-72h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket, so
After carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, room
Interior temperature controls between 25-35 DEG C, and relative humidity controls between 75-90%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap starts
Keratin, starts to eject spore, can gather by bagging.
Embodiment 8:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 80g, Ramulus Mori by weight
30g, Rhizoma Smilacis Glabrae 12g, Bombyx mori L. 12g, Spica Prunellae 10g, Herba Commelinae 10g；S2: cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L.,
Pulverizing after Spica Prunellae and Herba Commelinae mixing, measure the water content in compost after adding distilled water stirring, water content control is at 50-65%, and then heap is vexed
6-15h；After the vexed end of S3: heap, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, so
After seal again bag mouth be prepared as compost storage bag preserve 24-72h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket, so
After carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, room
Interior temperature controls between 25-35 DEG C, and relative humidity controls between 75-90%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap starts
Keratin, starts to eject spore, can gather by bagging.
Embodiment 9:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 100g, Mulberry by weight
Branch 60g, Rhizoma Smilacis Glabrae 40g, Bombyx mori L. 40g, Spica Prunellae 20g, Herba Commelinae 20g；S2: the cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, silkworm
Pulverizing after sand, Spica Prunellae and Herba Commelinae mixing, add the water content in mensuration compost after distilled water stirs, water content control is at 50-65%, then
The vexed 6-15h of heap；After the vexed end of S3: heap, compost is loaded in choke protective bag, pumps in choke protective bag after air, in bag, be filled with ammonia,
Seal bag mouth the most again and be prepared as compost storage bag preservation 24-72h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket,
Then carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf,
Indoor temperature controls between 25-35 DEG C, and relative humidity controls between 75-90%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap is opened
Beginning keratin, starts to eject spore, can gather by bagging.
Embodiment 10:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 120g, Mulberry by weight
Branch 50g, Rhizoma Smilacis Glabrae 20g, Bombyx mori L. 20g, Spica Prunellae 15g, Herba Commelinae 15g；S2: the cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, silkworm
Pulverizing after sand, Spica Prunellae and Herba Commelinae mixing, add the water content in mensuration compost after distilled water stirs, water content control is at 50-65%, then
The vexed 6-15h of heap；After the vexed end of S3: heap, compost is loaded in choke protective bag, pumps in choke protective bag after air, in bag, be filled with ammonia,
Seal bag mouth the most again and be prepared as compost storage bag preservation 24-72h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket,
Then carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf,
Indoor temperature controls between 25-35 DEG C, and relative humidity controls between 75-90%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap is opened
Beginning keratin, starts to eject spore, can gather by bagging.
Embodiment 11:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 50g, Ramulus Mori by weight
10g, Rhizoma Smilacis Glabrae 8g, Bombyx mori L. 8g, Spica Prunellae 5g, Herba Commelinae 5g；S2: the cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L., summer
Pulverizing after hay and Herba Commelinae mixing, measure the water content in compost after adding distilled water stirring, water content control is at 55-60%, and then heap is vexed
8-12h；After the vexed end of S3: heap, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, so
After seal again bag mouth be prepared as compost storage bag preserve 36-48h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket, so
After carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, room
Interior temperature controls between 28-32 DEG C, and relative humidity controls between 80-85%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap starts
Keratin, starts to eject spore, can gather by bagging.
Embodiment 12:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 300g, Mulberry by weight
Branch 80g, Rhizoma Smilacis Glabrae 50g, Bombyx mori L. 50g, Spica Prunellae 30g, Herba Commelinae 30g；S2: the cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, silkworm
Pulverizing after sand, Spica Prunellae and Herba Commelinae mixing, add the water content in mensuration compost after distilled water stirs, water content control is at 55-60%, then
The vexed 8-12h of heap；After the vexed end of S3: heap, compost is loaded in choke protective bag, pumps in choke protective bag after air, in bag, be filled with ammonia,
Seal bag mouth the most again and be prepared as compost storage bag preservation 36-48h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket,
Then carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf,
Indoor temperature controls between 28-32 DEG C, and relative humidity controls between 80-85%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap is opened
Beginning keratin, starts to eject spore, can gather by bagging.
Embodiment 13:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 80g, Ramulus Mori by weight
30g, Rhizoma Smilacis Glabrae 12g, Bombyx mori L. 12g, Spica Prunellae 10g, Herba Commelinae 10g；S2: cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L.,
Pulverizing after Spica Prunellae and Herba Commelinae mixing, measure the water content in compost after adding distilled water stirring, water content control is at 55-60%, and then heap is vexed
8-12h；After the vexed end of S3: heap, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, so
After seal again bag mouth be prepared as compost storage bag preserve 36-48h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket, so
After carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, room
Interior temperature controls between 28-32 DEG C, and relative humidity controls between 80-85%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap starts
Keratin, starts to eject spore, can gather by bagging.
Embodiment 14:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 100g, Mulberry by weight
Branch 60g, Rhizoma Smilacis Glabrae 40g, Bombyx mori L. 40g, Spica Prunellae 20g, Herba Commelinae 20g；S2: the cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, silkworm
Pulverizing after sand, Spica Prunellae and Herba Commelinae mixing, add the water content in mensuration compost after distilled water stirs, water content control is at 55-60%, then
The vexed 8-12h of heap；After the vexed end of S3: heap, compost is loaded in choke protective bag, pumps in choke protective bag after air, in bag, be filled with ammonia,
Seal bag mouth the most again and be prepared as compost storage bag preservation 36-48h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket,
Then carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf,
Indoor temperature controls between 28-32 DEG C, and relative humidity controls between 80-85%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap is opened
Beginning keratin, starts to eject spore, can gather by bagging.
Embodiment 15:
The cultural method of a kind of Ganoderma, it comprises the following steps: the preparation of S1: compost: weigh compost cotton seed hulls 120g, Mulberry by weight
Branch 50g, Rhizoma Smilacis Glabrae 20g, Bombyx mori L. 20g, Spica Prunellae 15g, Herba Commelinae 15g；S2: the cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, silkworm
Pulverizing after sand, Spica Prunellae and Herba Commelinae mixing, add the water content in mensuration compost after distilled water stirs, water content control is at 55-60%, then
The vexed 8-12h of heap；After the vexed end of S3: heap, compost is loaded in choke protective bag, pumps in choke protective bag after air, in bag, be filled with ammonia,
Seal bag mouth the most again and be prepared as compost storage bag preservation 36-48h；S4: the compost in S3 step storage bag is loaded polypropylene kind pocket,
Then carry out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf,
Indoor temperature controls between 28-32 DEG C, and relative humidity controls between 80-85%, and indoor holding is dark；S6: when Ganoderma cap launches, and cap is opened
Beginning keratin, starts to eject spore, can gather by bagging.
Below by testing the effect further illustrating the present invention:
The impact that Ganderma lucidum is grown by experiment one, compost of the present invention and cultural method
1 materials and methods
Culture material formula: experiment A group: cotton seed hulls 20kg, Ramulus Mori 8kg, Rhizoma Smilacis Glabrae 5kg, Bombyx mori L. 5kg, Spica Prunellae 3kg, Herba Commelinae 3kg；
Experiment B group: cotton seed hulls 12kg, Ramulus Mori 5kg, Rhizoma Smilacis Glabrae 2kg, Bombyx mori L. 2kg, Spica Prunellae 1.5kg, Herba Commelinae 1.5kg；Experiment C group: Semen Gossypii
Shell 10kg, Ramulus Mori 3kg, Rhizoma Smilacis Glabrae 1.2kg, Bombyx mori L. 1.2kg, Spica Prunellae 1kg, Herba Commelinae 1kg；Experiment D group: cotton seed hulls culture medium: cotton
Seed shell 10kg, Testa Tritici 1kg, glucose 2kg, agar 2kg；Experiment E group: maize powder medium: Semen Maydis powder 5kg, peptone 0.5kg, MgSO40.05kg、
KH2PO40.15kg, glucose 2kg, agar 2kg.Experiment A, B, C group concrete preparation method, by cotton seed hulls, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L.,
Pulverizing after Spica Prunellae and Herba Commelinae mixing, measure the water content in compost after adding distilled water stirring, water content control is at 50-65%, and then heap is vexed
6-15h；After vexed for heap end, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, then
Seal bag mouth again and be prepared as compost storage bag preservation 24-72h；Compost in S3 step storage bag is loaded polypropylene kind pocket, then carries out
Conventional sterilization treatment, is inoculated into polypropylene kind pocket by Ganderma lucidum strain.The cotton seed hulls culture medium of experiment D group and the maize powder medium of experiment E group
The most conventionally it is prepared as polypropylene kind pocket.
2 experimental results
After preparing the culture medium of each experimental group, by lucidum seed bacterium subcultivation to polypropylene kind pocket, quantitatively access flat board tip bacterium with the card punch of sterilizing
Silk, each formula connects 2O planting bag, and room temperature controls to cultivate in 25-28 DEG C, observes and measure mycelial growth rate, mycelial density, mycelium
Cover with the natural law of culture medium, the results are shown in Table 1.From subcultivation to the yield of results Ganoderma and bacterial contamination rate, the results are shown in Table 2.
Table 1 different culture media growth effect to Ganoderma
Result shows, the speed of growth of experimental group A, B, C group of the present invention is faster than experiment D group and the speed of growth of E group, and covers with the sky of culture medium
Number is less than experiment D group and the natural law of E group.
Table 2 different culture media is on Ganoderma yield and the impact of bacterial contamination rate
Result shows, the yield of experimental group A, B, C group of the present invention is apparently higher than experiment D, E group, and difference has statistical significance (P ＜ 0.05)；
The bacterial contamination rate of experiment B group is minimum, is closer to experiment A group and C group, there was no significant difference, and compares with experiment D combination E group, has
Significant difference (P ＜ 0.05), shows that testing B group culture medium prescription can effectively suppress the germ reproduction of Ganoderma substrate, can effectively reduce spirit
Sesame strain infects parasite or the probability of pathogenic bacteria.
Claims (7)
1. the cultural method of a Ganoderma, it is characterised in that: it comprises the following steps:
The preparation of S1: compost: weigh compost cotton seed hulls 50-300 weight portion, Ramulus Mori 10-80 weight portion, Rhizoma Smilacis Glabrae 8-50 weight portion, Bombyx mori L. 8-50 weight portion, Spica Prunellae 5-30 weight portion, Herba Commelinae 5-30 weight portion by weight；
Pulverize after S2: cotton seed hulls in S1 step, Ramulus Mori, Rhizoma Smilacis Glabrae, Bombyx mori L., Spica Prunellae and Herba Commelinae mixing, after adding distilled water stirring, measure the water content in compost, water content control at 50-65%, the then vexed 6-15h of heap；
After the vexed end of S3: heap, compost is loaded in choke protective bag, pump in choke protective bag after air, in bag, be filled with ammonia, seal bag mouth the most again and be prepared as compost storage bag and preserve 24-72h；
S4: the compost in S3 step storage bag is loaded polypropylene kind pocket, then carries out sterilization treatment, Ganderma lucidum strain is inoculated into polypropylene kind pocket；
S5: by postvaccinal for S4 step kind of pocket traverse in fungus shelf, indoor temperature controls between 25-35 DEG C, and relative humidity controls between 75-90%, indoor holding is dark；
S6: when Ganoderma cap launches, and cap starts keratin, starts to eject spore, can gather by bagging.
The cultural method of a kind of Ganoderma the most according to claim 1, it is characterised in that: compost and weight proportion thereof in S1 step be: cotton seed hulls 80-200 weight portion, Ramulus Mori 10-80 weight portion, Rhizoma Smilacis Glabrae 8-50 weight portion, Bombyx mori L. 8-50 weight portion, Spica Prunellae 5-30 weight portion, Herba Commelinae 5-30 weight portion.
The cultural method of a kind of Ganoderma the most according to claim 1, it is characterised in that: compost and weight proportion thereof in S1 step be: cotton seed hulls 100-150 weight portion, Ramulus Mori 30-60 weight portion, Rhizoma Smilacis Glabrae 12-40 weight portion, Bombyx mori L. 12-40 weight portion, Spica Prunellae 10-20 weight portion, Herba Commelinae 10-20 weight portion.
The cultural method of a kind of Ganoderma the most according to claim 1, it is characterised in that: compost and weight proportion thereof in S1 step be: cotton seed hulls 120 weight portion, Ramulus Mori 50 weight portion, Rhizoma Smilacis Glabrae 20 weight portion, Bombyx mori L. 20 weight portion, Spica Prunellae 15 weight portion, Herba Commelinae 15 weight portion.
The cultural method of a kind of Ganoderma the most according to claim 1, it is characterised in that: the water content in S2 step compost at 55-60%, the then vexed 8-12h of heap.
The cultural method of a kind of Ganoderma the most according to claim 1; it is characterized in that: compost is loaded in choke protective bag after the vexed end of S3 step heap; pump in choke protective bag after air, in bag, be filled with ammonia, seal bag mouth the most again and be prepared as compost storage bag and preserve 36-48h.
The cultural method of a kind of Ganoderma the most according to claim 1, it is characterised in that: postvaccinal kind of pocket traverse of S4 step is in fungus shelf, and indoor temperature controls between 28-32 DEG C, and relative humidity controls between 80-85%.
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| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
| CN105646067A (en) * | 2015-12-30 | 2016-06-08 | 桂林大野领御生物科技有限公司 | Lucid ganoderma cultivation method for promoting mycelial growth |
| CN107455142A (en) * | 2017-08-29 | 2017-12-12 | 田林县群英农业有限公司 | A kind of implantation methods for improving ganoderma lucidum quality |
| CN107382520A (en) * | 2017-08-29 | 2017-11-24 | 田林县群英农业有限公司 | A kind of Medium for Ganoderma lucidum and preparation method thereof |
| CN107567950A (en) * | 2017-08-29 | 2018-01-12 | 田林县群英农业有限公司 | A kind of cultural method of ganoderma lucidum |
| CN108575549A (en) * | 2018-03-15 | 2018-09-28 | 贵州原生态生物科技有限公司 | A kind of simulated wild glossy ganoderma cultivating superior high-yield method |
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| CN103460990A (en) * | 2013-08-05 | 2013-12-25 | 滁州市南谯区大柳镇凤胜食用菌专业合作社 | Cultivation method for blood-sugar-reduction health-care ganoderma lucidum |
| CN103460983A (en) * | 2013-08-05 | 2013-12-25 | 滁州市南谯区大柳镇凤胜食用菌专业合作社 | Cultivation method for liver-protection health-care ganoderma lucidum |
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