CN105646067A - Lucid ganoderma cultivation method for promoting mycelial growth - Google Patents
Lucid ganoderma cultivation method for promoting mycelial growth Download PDFInfo
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- CN105646067A CN105646067A CN201511019522.7A CN201511019522A CN105646067A CN 105646067 A CN105646067 A CN 105646067A CN 201511019522 A CN201511019522 A CN 201511019522A CN 105646067 A CN105646067 A CN 105646067A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
Abstract
The invention provides a lucid ganoderma cultivation method for promoting mycelial growth. The lucid ganoderma cultivation method comprises strain culture, culture medium preparation, mycelium culture and ganoderma lucidum growth management, wherein the culture medium preparation comprises mixing, smashing, bagging and sterilizing the following raw materials in parts by weight: 30 to 35 parts of weed tree sawdust, 10 to 20 parts of corncobs, 5 to 10 parts of rice bran, 1 to 3 parts of burdock, 2 to 4 parts of grifola frondosa and 0.5 to 1.5 parts of milk sugar. According to the lucid ganoderma cultivation method, the grifola frondosa, burdock and milk sugar are added into a conventional culture medium, the activity of alpha-glucophosphomutase can be improved, and branch migration of lucid ganoderma cell metabolism to ganoderan synthesis preferably is promoted, so that the content of ganoderma exopolysaccharides is greatly increased; meanwhile, the growth of lucid ganoderma can be promoted, and the hypha bagful time is greatly shortened.
Description
Technical field
The present invention relates to the technical field of cultivation of Ganoderma, be specifically related to a kind of ganoderma lucidum cultivation method promoting mycelial growth.
Background technology
Ganoderma (Ganodermalucidum) is commonly called as Ganoderma lucidum seu Japonicum, is the medicinal fungi of a kind of preciousness. It has significantly high medical value, and human body is had health-care effect, and disease has prevention, treatment or inhibitory action. The sporophore (or sclerotium) that traditional field acquisition or artificial culture produce receives that wild resource is rare, sporophore cultivation and production cycle length (4��May), produce the factors restrictions such as the conditions such as climate control and bioactive substance content is low, and a large amount of production of Ganoderma is caused obstruction. Ganoderan is effective chemical substance that Ganoderma is main, homopolysaccharide that to be a class be formed by connecting with ��-(1,3) and ��-(Isosorbide-5-Nitrae) key or heteropolysaccharide macromolecular substances. Numerous researchs show, ganoderan has the biological activitys such as adjustment immunocompetence, antitumor, antioxidation, AntiHIV1 RT activity, blood pressure lowering.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of ganoderma lucidum cultivation method promoting mycelial growth, and the method can be substantially reduced the mycelia purseful time, it is also possible to improve the content of polysaccharide in Ganoderma sporophore.
Technical scheme provided by the invention is a kind of ganoderma lucidum cultivation method promoting mycelial growth, including spawn culture, medium preparing, mycelia culture, going out sesame management, described medium preparing is the raw material co-grinding of following weight portion, pack, sterilizing to be formed: weed tree sawdust 30��35 parts, corn cob 10��20 parts, 5��10 parts of Testa oryzae, Fructus Arctii 1��3 part, Grifola frondosa 2��4 parts and lactose 0.5��1.5 part.
Grifola frondosa (Grifolafrinosa), is a kind of medicine-food two-purpose bacterium rich in effective ingredient such as polysaccharide, vitamin, aminoacid, protein, inorganic salts.
Weed tree sawdust, corn cob, Testa oryzae are conventional culture medium raw materials, on this basis, also added Fructus Arctii, Grifola frondosa and lactose, the interpolation of Fructus Arctii, Grifola frondosa and lactose can improve the activity of alpha-phosphate glucose mutase, and alpha-phosphate glucose mutase take part in the synthesis of extracellular polysaccharide, alpha-phosphate glucose mutase activity strengthens, bootable G-6-P carries out to polysaccharide metabolic pathway of synthesizing direction, the branch road that the metabolism of glossy ganoderma cell synthesizes more towards ganoderan is promoted to migrate, thus being greatly increased the content of Ganoderma extracellular polysaccharide. Moreover, Fructus Arctii, Grifola frondosa and lactose composite after, it is also possible to promote Ganoderma growth.
The moisture content of above-mentioned culture medium is 60��65%.
Above-mentioned mycelia culture is that culture medium is placed in gnotobasis inoculation strain, 22��25 DEG C, relative humidity be under 60��70% conditions, be cultured to mycelia purseful.
Above-mentioned go out sesame management be the bacterium bag covering with mycelia is placed in 25��28 DEG C, relative humidity be 80��90%, intensity of illumination be cultured to sporophore maturation when being 1000��2000lux, can gather.
Compared with prior art, the method have the advantages that
1) present invention with the addition of Grifola frondosa, Fructus Arctii and lactose in conventional medium, the activity of alpha-phosphate glucose mutase can be improved, the branch road that the metabolism of glossy ganoderma cell synthesizes more towards ganoderan is promoted to migrate, thus being greatly increased the content of Ganoderma extracellular polysaccharide.
2) after the composite interpolation of Grifola frondosa, Fructus Arctii and lactose, it is also possible to promote Ganoderma growth, be greatly shortened the mycelia purseful time.
Specific embodiment mode
The present invention is further elaborated for specific examples below, but not as a limitation of the invention.
Embodiment 1
1) strain selects: adopt tissue isolation to obtain pure female kind, by wild or tame just after a part of 75v% ethanol of the fresh Han Zhi sporophore of growth promoter carries out surface sterilization, the fritter of 3��5mm it is cut into by aseptic manipulation, take about 5 pieces to be positioned on plating medium, or take 1 piece and be placed in test tube slant culture medium. Cultivating 3��4 days at 25��28 DEG C, when little block organization surrounding white mycelia grows, select and pure white be transplanted on new slant medium without assorted mycelia, continue to cultivate about 5 days, obtain lucid ganoderma stock culture, then amplification culture becomes original seed and cultigen again.
2) medium preparing: by the raw material of following weight portion: weed tree sawdust 30 parts, corn cob 10 parts, 5 parts of Testa oryzae, Fructus Arctii 1 part, 2 parts of lactose of Grifola frondosa 0.5 part; By above-mentioned raw materials co-grinding, adding water to moisture content is 60%, can pack. Every bag of siccative 350��450g, Bag Material normal-pressure sterilization 10h, be cooled to 30 DEG C and be carried out below aseptic inoculation. The bacterium bag inoculated is placed on indoor cultivation, maintain the temperature at 22 DEG C, relative humidity be 60%, when mycelium sends out full 2/3, move into and cultivate in canopy, indoor astigmatism, until mycelia purseful.
3) after mycelia sent out full by Ganderma lucidum bag, be placed in 25 DEG C, relative humidity be 80%, intensity of illumination be cultured to sporophore maturation when being 1000lux, can gather.
Reference examples 1
Identical with embodiment 1, only the raw material of culture medium is different, and the raw material of the culture medium of this reference examples includes the raw material of following weight portion and makes: weed tree sawdust 30 parts, corn cob 10 parts and 5 parts of Testa oryzae.
Embodiment 2
1) strain selects: adopt tissue isolation to obtain pure female kind, by wild or tame just after a part of 75v% ethanol of the fresh ganoderma lucidum sporocarp of growth promoter carries out surface sterilization, the fritter of 3��5mm it is cut into by aseptic manipulation, take about 5 pieces to be positioned on plating medium, or take 1 piece and be placed in test tube slant culture medium. Cultivating 3��4 days at 25��28 DEG C, when little block organization surrounding white mycelia grows, select and pure white be transplanted on new slant medium without assorted mycelia, continue to cultivate about 5 days, obtain lucid ganoderma stock culture, then amplification culture becomes original seed and cultigen again.
2) by the raw material of following weight portion: weed tree sawdust 35 parts, corn cob 20 parts, 10 parts of Testa oryzae, Fructus Arctii 3 parts, Grifola frondosa 4 parts and lactose 1.5 parts; By above-mentioned raw materials co-grinding, adding water to moisture content is 65%, can pack.Every bag of siccative 350��450g, Bag Material normal-pressure sterilization 10h, be cooled to 30 DEG C and be carried out below aseptic inoculation. The bacterium bag inoculated is placed on indoor cultivation, maintain the temperature at 25 DEG C, relative humidity be 70%, when mycelium sends out full 2/3, move into and cultivate in canopy, indoor astigmatism, until mycelia purseful.
3) after mycelia sent out full by Ganderma lucidum bag, be placed in 25��28 DEG C, relative humidity be 80��90%, intensity of illumination be cultured to sporophore maturation when being 1000��2000lux, can gather.
Reference examples 2
Identical with embodiment 2, only the raw material of culture medium is different, and the raw material of the culture medium of this reference examples includes the raw material of following weight portion and makes: weed tree sawdust 35 parts, corn cob 20 parts and 10 parts of Testa oryzae.
Embodiment 3
1) strain selects: adopt tissue isolation to obtain pure female kind, by wild or tame just after a part of 75v% ethanol of the new fresh and sweet sesame sporophore of growth promoter carries out surface sterilization, the fritter of 3��5mm it is cut into by aseptic manipulation, take about 5 pieces to be positioned on plating medium, or take 1 piece and be placed in test tube slant culture medium. Cultivating 3��4 days at 25��28 DEG C, when little block organization surrounding white mycelia grows, select and pure white be transplanted on new slant medium without assorted mycelia, continue to cultivate about 5 days, obtain lucid ganoderma stock culture, then amplification culture becomes original seed and cultigen again.
2) by the raw material of following weight portion: weed tree sawdust 32 parts, corn cob 15 parts, 8 parts of Testa oryzae, Fructus Arctii 2 parts, Grifola frondosa 3 parts and lactose 1 part; By above-mentioned raw materials co-grinding, add water to moisture 62%, can pack. Every bag of siccative 350��450g, Bag Material normal-pressure sterilization 10h, be cooled to 30 DEG C and be carried out below aseptic inoculation. The bacterium bag inoculated is placed on indoor cultivation, maintain the temperature at 24 DEG C, relative humidity be 65%, when mycelium sends out full 2/3, move into and cultivate in canopy, indoor astigmatism, until mycelia purseful.
3) after mycelia sent out full by Ganderma lucidum bag, be placed in 26 DEG C, relative humidity be 85%, intensity of illumination be cultured to sporophore maturation when being 1500lux, can gather.
Reference examples 3
Identical with embodiment 3, only the raw material of culture medium is different, and the raw material of the culture medium of this reference examples includes the raw material of following weight portion and makes: weed tree sawdust 32 parts, corn cob 15 parts and 8 parts of Testa oryzae.
Reference examples 4
Identical with embodiment 3, only the raw material of culture medium is different, and the raw material of the culture medium of this reference examples includes the raw material of following weight portion and makes: weed tree sawdust 32 parts, corn cob 15 parts, 8 parts of Testa oryzae and Fructus Arctii 2 parts.
Reference examples 5
Identical with embodiment 3, only the raw material of culture medium is different, and the raw material of the culture medium of this reference examples includes the raw material of following weight portion and makes: weed tree sawdust 32 parts, corn cob 15 parts, 8 parts of Testa oryzae and Grifola frondosa 3 parts.
Reference examples 6
Identical with embodiment 3, only the raw material of culture medium is different, and the raw material of the culture medium of this reference examples includes the raw material of following weight portion and makes: weed tree sawdust 32 parts, corn cob 15 parts, 8 parts of Testa oryzae and lactose 1 part.
9, the mycelial growth synopsis of embodiment 1��3 and reference examples 1��3
2, the content synopsis of embodiment 1��3 and reference examples 1��3 separate sources ganoderan
| Solid mycelia (%) | Sporophore (%) | |
| Embodiment 1 | 6.15 | 3.59 |
| Reference examples 1 | 3.35 | 1.28 |
| Embodiment 2 | 6.70 | 3.69 |
| Reference examples 2 | 3.11 | 1.15 |
| Embodiment 3 | 8.55 | 6.13 |
| Reference examples 3 | 4.03 | 1.58 |
| Reference examples 4 | 4.86 | 1.64 |
| Reference examples 5 | 4.92 | 1.89 |
| Reference examples 6 | 5.06 | 2.13 |
Claims (4)
1. the ganoderma lucidum cultivation method promoting mycelial growth, including spawn culture, medium preparing, mycelia culture, go out sesame management, it is characterised in that: described medium preparing is the raw material co-grinding of following weight portion, pack, sterilizing to be formed: weed tree sawdust 30��35 parts, corn cob 10��20 parts, 5��10 parts of Testa oryzae, Fructus Arctii 1��3 part, Grifola frondosa 2��4 parts and lactose 0.5��1.5 part.
2. a kind of ganoderma lucidum cultivation method promoting mycelial growth according to claim 1, it is characterised in that: the moisture content of described culture medium is 60��65%.
3. a kind of ganoderma lucidum cultivation method promoting mycelial growth according to claim 1, it is characterized in that: described mycelia culture is that culture medium is placed in gnotobasis inoculation strain, 22��25 DEG C, relative humidity be under 60��70% conditions, be cultured to mycelia purseful.
4. a kind of ganoderma lucidum cultivation method promoting mycelial growth according to claim 1, it is characterized in that: described in go out sesame management be the bacterium bag covering with mycelia is placed in 25��28 DEG C, relative humidity be 80��90%, intensity of illumination be cultured to sporophore maturation when being 1000��2000lux, can gather.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106072491A (en) * | 2016-06-20 | 2016-11-09 | 张勇 | A kind of Fructus Arctii base Ganoderma lucidum mycelium powder and preparation method thereof |
| CN106929427A (en) * | 2017-01-10 | 2017-07-07 | 北京林业大学 | It is suitable to the culture medium and its cultural method of medicinal fungi ganoderma lucidum high-yield extracellular polysaccharide |
| CN107012101A (en) * | 2017-04-18 | 2017-08-04 | 徐州工程学院 | A kind of method for removing the peel burdock liquid fermentation medium and the new mushroom of fermented tea |
| CN108967616A (en) * | 2018-08-23 | 2018-12-11 | 广西生命之菌生物科技有限公司 | A kind of Ganoderma lucidum mycelium nutraceutical |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106072491A (en) * | 2016-06-20 | 2016-11-09 | 张勇 | A kind of Fructus Arctii base Ganoderma lucidum mycelium powder and preparation method thereof |
| CN106072491B (en) * | 2016-06-20 | 2019-10-08 | 张勇 | A kind of fructus arctii base Ganoderma lucidum mycelium powder and preparation method thereof |
| CN106929427A (en) * | 2017-01-10 | 2017-07-07 | 北京林业大学 | It is suitable to the culture medium and its cultural method of medicinal fungi ganoderma lucidum high-yield extracellular polysaccharide |
| CN106929427B (en) * | 2017-01-10 | 2020-09-08 | 北京林业大学 | Culture medium suitable for high-yield extracellular polysaccharide of medicinal fungus ganoderma lucidum and culture method thereof |
| CN107012101A (en) * | 2017-04-18 | 2017-08-04 | 徐州工程学院 | A kind of method for removing the peel burdock liquid fermentation medium and the new mushroom of fermented tea |
| CN108967616A (en) * | 2018-08-23 | 2018-12-11 | 广西生命之菌生物科技有限公司 | A kind of Ganoderma lucidum mycelium nutraceutical |
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