CN105248150A - Method for increasing content of cordycepin in cordyceps militaris sporocarp - Google Patents
Method for increasing content of cordycepin in cordyceps militaris sporocarp Download PDFInfo
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- CN105248150A CN105248150A CN201510803101.7A CN201510803101A CN105248150A CN 105248150 A CN105248150 A CN 105248150A CN 201510803101 A CN201510803101 A CN 201510803101A CN 105248150 A CN105248150 A CN 105248150A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention relates to a method for increasing the content of cordycepin in cordyceps militaris sporocarp. It is required that high-temperature stress treatment is adopted in the particular period of culture of the cordyceps militaris sporocarp, namely from the 40th day to the 50th day, the culture temperature is raised to 24-30 DEG C from 15-20 DEG C to be kept for 10-20 days, harvesting is conducted on the day ranging from the 55th day to the 65th day, and the cordyceps militaris sporocarp with the cordycepin content reaching 0.5% can be obtained. According to the method, from the 40th-50th day of cordyceps militaris sporocarp culture, high-temperature stress treatment is adopted; meanwhile, strong white light or strong blue light stimulation treatment is assisted, and the content of the cordycepin in the cordyceps militaris sporocarp can be further increased and can reach 0.6%-0.9% and 0.9%-1.5%. The method has great innovativeness and application value.
Description
Technical field
The present invention relates to Artificial Planting Method of C. militarles field, specifically a kind of artificial culture method improving cordycepin content in fruiting bodies of cordyceps militaris.
Background technology
Cordyceps militaris (
cordycepsmilitaris (L.) Link) be under the jurisdiction of Eumycota (Eumycota), Ascomycota (Ascomycota), Ascomycotina (Ascomycotinia), excrement shell Gammaproteobacteria (Sordariomycetes), meat seat bacterium subclass (Hypocreomycetidae), Hypocreales (Hypocreales), Chinese caterpillar fungus section (Cordycipitaceae), Cordyceps (
cordyceps).Cordyceps militaris has another name called northern Chinese caterpillar Fungus, to Cordyceps sinensis, there is similar bioactive ingredients, mainly comprise cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide, ergosterol, amino acid, nucleosides material etc., effect is similar to Cordyceps sinensis, be the good substitute of Cordyceps sinensis, and cordycepin content is also higher than Cordyceps sinensis.
Cordycepin (cordycepin) is i.e. cordycepin (3 '-deoxyadenosine), also known as cordycepin, is first the ucleosides antibiotic separated from fungi.Be found and be separated to obtain sterling and start since mid-term in 20th century, the biological action of people's just continuous research cordycepin, comprise the effects such as antitumor, immunological regulation, antibacterial anti-inflammatory, resisting vascular smooth muscle, there is very high health care and medicinal study is worth.For this reason, in March, 2009 health ministry issue 09 year No. 3 bulletin approval Cordyceps militaris be new resource food, in May, 2014 national health State Family Planning Commission issue 14 years No. 10 bulletin approval Cordyceps militaris be new raw-food material, compare cordyceps mycelium, the fruiting bodies of cordyceps militaris of artificial cultivation can be used as bread and cheese raw material, can normally be used in food and health food.And at present in raising fruiting bodies of cordyceps militaris in cordycepin content, mainly concentrate on the modes such as strain improvement, liquid and solid culture medium formulation optimization, and the condition of culture optimized also needs the problem such as cost, cycle considering large-scale production, thus in fruiting bodies of cordyceps militaris, cordycepin content is difficult to generally break through, and also pins down the application of cordycepin and the road of industrialization development deeply.
Through the Searches of Patent Literature, patent No. CN201310541025.8 " improving the cordyceps culturing method of cordycepin content ", it optimizes solid culture medium, liquid nutrient medium and fermentation broth formula, the mode of Treatment with High Concentration Zinc ionic stress is utilized to improve cordycepin content in fruiting bodies of cordyceps militaris, and this technical scheme to promote amplitude not high, in preferred embodiment example, most high-load is only 0.21%; Application number CN201310217705.4 " the Cordceps militaris cultural method of cordycepin content can be improved ", it adopts rice, silky ant powder etc. as Optimal Medium, and utilize the mode of 10 DEG C of thermal stimulations round the clock to realize improving cordycepin content in fruiting bodies of cordyceps militaris, and silky ant raw material sources are in short supply in this technical scheme, and its quality also cannot effectively control, and is unsuitable for large-scale production and application; Patent No. CN200610117998.9 " a kind of industrial method for culturing north winter of worm summer herb with high-content of cordycepin ", it is on the basis of strain excellent COB201, CGMCCNo.1823, and optimize original seed liquid nutrient medium, realize improving cordycepin content in fruiting bodies of cordyceps militaris with this, and Cordyceps militaris spawn is easier to degenerate, for most those of ordinary skill, bacterial classification sieve is educated and needs at substantial time and energy, and extensive use exists difficult.And the technical scheme that this patent proposes, it is that the moment requiring to cultivate Cordyceps militaris changes condition of culture, to promote cordycepin content in fruiting bodies of cordyceps militaris.The method is simple and easy, is easy to realize, and does not affect steady production, can significantly promote cordycepin content in fruiting bodies of cordyceps militaris, successful, also be different from the mode that above strain improvement, liquid and solid culture medium formulation optimization etc. are comparatively loaded down with trivial details and limited.
The present invention is directed to the present situation that in present stage large-scale production, in fruiting bodies of cordyceps militaris, cordycepin content level is generally lower, a kind of method improving cordycepin content in fruiting bodies of cordyceps militaris is provided, the moment of namely cultivating Cordyceps militaris changes condition of culture, to realize the content promoting cordycepin in fruiting bodies of cordyceps militaris, the method also can superpose with current existing technical scheme and use, and has novel creativeness and using value widely.
Summary of the invention
The object of this invention is to provide a kind of method improving cordycepin content in fruiting bodies of cordyceps militaris, it is mainly through utilizing high temperature stress in moment, or is aided with the mode of high light stimulation again, to improve cordycepin content in fruiting bodies of cordyceps militaris.
The technical solution used in the present invention is for this reason: what the present invention cultivated at fruiting bodies of cordyceps militaris starts for 40-50 days, take high temperature stress process, or be aided with strong white light or strong blue light stimulation again, keep 10-20 days, gathered to 55-65 days, the program can significantly improve cordycepin content in fruiting bodies of cordyceps militaris.
Technical scheme of the present invention is mainly following steps:
(1) medium is prepared:
Its recipe ingredient is by weight: the grain raw material such as rice or wheat 30-45 part, dried silkworm chrysalis meal 6-10 part, nutrient solution 60-80 part, pH value 6-8; Its nutrient solution prescription is by weight: carbohydrate 45-90 part, peptone 45-90 part, potassium dihydrogen phosphate 4.5-9 part, magnesium sulfate 4.5-9 part, B B-complex 3-5 part, water 6000-7000 part;
(2) sterilizing:
By the medium sealing prepared, be placed in high pressure moist heat sterilization pot, 121-125 DEG C, 60-80min;
(3) inoculate:
After medium temperature is low to moderate 18-25 DEG C, under aseptic condition, access Cordyceps militaris spawn;
(4) bacterium is sent out:
Be placed in cultivating chamber by postvaccinal medium and cultivate, temperature 15-20 DEG C, humidity 60-80%, light culture 4-8 days until a bacterium is complete;
(5) fruit body development management:
Giving 100-300lux illumination completely when sending out bacterium, keeping temperature 15-20 DEG C, humidity 60-80%; Within 8-12 days, mycelia annesl is complete, within 14-20 days, can form stroma bud; During 40-50 days, adopt high temperature stress processing mode, by temperature increase to 24-30 DEG C, or be aided with strong white light, strong blue light stimulation process again, keep 10-20 days;
(6) gather, dry
Within 55-65 days, gather; Dry at 45-55 DEG C to fruiting bodies of cordyceps militaris water content and reach less than 10%, package storage.
Advantage of the present invention is:
What 1, the present invention specified in that fruiting bodies of cordyceps militaris manually cultivates starts for 40-50 days, take high temperature stress process, or be aided with strong white light or strong blue light stimulation process again, the cordycepin content in fruiting bodies of cordyceps militaris can be made significantly to increase: the mode of moment high temperature stress process, cordycepin content in fruiting bodies of cordyceps militaris can be made to be promoted to more than 0.5% by 0.1-0.3%; The mode of moment high temperature stress and strong White-Light processing, can make cordycepin content in fruiting bodies of cordyceps militaris be promoted to 0.6-0.9% by 0.1-0.3%; The mode of moment high temperature stress and strong blue light process, can make cordycepin content in fruiting bodies of cordyceps militaris be promoted to 0.9-1.5% by 0.1-0.3%.By the technical program, the general lower present situation of cordycepin content in fruiting bodies of cordyceps militaris can be improved.
2, present invention process is simple, is easy to realize, and on the basis not affecting Cordyceps militaris output, can promote cordycepin content in fruiting bodies of cordyceps militaris, be beneficial to extensive cultivation.
3, the present invention also can superpose with current existing technical method and use, and implements flexible, applied range.
Embodiment is as follows:
Embodiment 1
(1) medium is prepared:
Its formula is: rice 35g, dried silkworm chrysalis meal 7g, nutrient solution 65ml, pH value 6-8; Its nutrient solution prescription is: carbohydrate 800g, peptone 800g, potassium dihydrogen phosphate 45g, magnesium sulfate 90g, B B-complex 30g, water 65kg;
(2) sterilizing:
By the medium sealing prepared, be placed in high pressure moist heat sterilization pot, 123 DEG C, 80min;
(3) inoculate:
After medium temperature is low to moderate 18-25 DEG C, under aseptic condition, access Cordyceps militaris spawn;
(4) bacterium is sent out:
Be placed in cultivating chamber by postvaccinal medium and cultivate, temperature 15-20 DEG C, humidity 60-80%, light culture 4-8 days until a bacterium is complete;
(5) fruit body development management:
Giving 250lux white light completely, 24 hours/day when sending out bacterium, keeping temperature 15-20 DEG C, humidity 60-80%; When the 45th day, adopt high temperature stress process, by temperature increase to 25 DEG C, keep 10 days;
(6) gather, dry
After within 55th day, gathering, dry when reaching below 10% to fruiting bodies of cordyceps militaris water content at 45-55 DEG C, package storage.
Embodiment 2
(1) medium is prepared:
Its formula is: rice 35g, dried silkworm chrysalis meal 7g, nutrient solution 65ml, pH value 6-8; Its nutrient solution prescription is: carbohydrate 800g, peptone 800g, potassium dihydrogen phosphate 45g, magnesium sulfate 90g, B B-complex 30g, water 65kg;
(2) sterilizing:
By the medium sealing prepared, be placed in high pressure moist heat sterilization pot, 123 DEG C, 80min;
(3) inoculate:
After medium temperature is low to moderate 18-25 DEG C, under aseptic condition, access Cordyceps militaris spawn;
(4) bacterium is sent out:
Be placed in cultivating chamber by postvaccinal medium and cultivate, temperature 15-20 DEG C, humidity 60-80%, light culture 4-8 days until a bacterium is complete;
(5) fruit body development management:
Giving 250lux white light completely, 24 hours/day when sending out bacterium, keeping temperature 15-20 DEG C, humidity 60-80%; When the 49th day, adopt high temperature stress and strong white light stimulation process, by temperature increase to 26 DEG C, light intensity increases to 4800lux, 24 hours/day, keeps 15 days;
(6) gather, dry
After within 64th day, gathering, dry when reaching below 10% to fruiting bodies of cordyceps militaris water content at 45-55 DEG C, package storage.
Embodiment 3
(1) medium is prepared:
Its formula is: rice 35g, dried silkworm chrysalis meal 7g, nutrient solution 65ml, pH value 6-8; Its nutrient solution prescription is: carbohydrate 800g, peptone 800g, potassium dihydrogen phosphate 45g, magnesium sulfate 90g, B B-complex 30g, water 65kg;
(2) sterilizing:
By the medium sealing prepared, be placed in high pressure moist heat sterilization pot, 123 DEG C, 80min;
(3) inoculate:
After medium temperature is low to moderate 18-25 DEG C, under aseptic condition, access Cordyceps militaris spawn;
(4) bacterium is sent out:
Be placed in cultivating chamber by postvaccinal medium and cultivate, temperature 15-20 DEG C, humidity 60-80%, light culture 4-8 days until a bacterium is complete;
(5) fruit body development management:
Giving 250lux white light completely, 24 hours/day when sending out bacterium, keeping temperature 15-20 DEG C, humidity 60-80%; When the 43rd day, adopt high temperature stress and strong blue light stimulation process, by temperature increase to 24 DEG C, illumination is replaced by 4000lux blue light, 24 hours/day, keeps 20 days;
(6) gather, dry
After within 63rd day, gathering, dry when reaching below 10% to fruiting bodies of cordyceps militaris water content at 45-55 DEG C, package storage.
Comparative example:
Be with the important difference of embodiment 1-3, in comparative example incubation, do not take high temperature stress or in conjunction with high light stimulation process in moment.
Table 1: described moment of cultivating at fruiting bodies of cordyceps militaris, namely within 40-50 days, rise, take high temperature stress process, cultivation temperature is increased to 24-30 DEG C by 15-20 DEG C, or be aided with strong white light, strong blue light stimulation process again, keep 10-20 days, gathered to 55-65 days, this technical method is on the impact of fruiting bodies of cordyceps militaris dry weight and cordycepin content.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the mode that the present invention takes; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (5)
1. improve a method for cordycepin content in fruiting bodies of cordyceps militaris, it is characterized in that, require the moment of cultivating at fruiting bodies of cordyceps militaris, namely within 40-50 days, rise, take high temperature stress process, cultivation temperature is increased to 24-30 DEG C by 15-20 DEG C, keep 10-20 days, gathered to 55-65 days.
2. the method for cordycepin content in a kind of effective raising fruiting bodies of cordyceps militaris according to claim 1, is characterized in that, mainly comprise the following steps:
(1) medium is prepared:
Its recipe ingredient is by weight: the grain raw material such as rice or wheat 30-45 part, dried silkworm chrysalis meal 6-10 part, nutrient solution 60-80 part, pH value 6-8; Its nutrient solution prescription is by weight: carbohydrate 45-90 part, peptone 45-90 part, potassium dihydrogen phosphate 4.5-9 part, magnesium sulfate 4.5-9 part, B B-complex 3-5 part, water 6000-7000 part;
(2) sterilizing:
By the medium sealing prepared, be placed in high pressure moist heat sterilization pot, 121-125 DEG C, 60-80min;
(3) inoculate:
After medium temperature is low to moderate 18-25 DEG C, under aseptic condition, access Cordyceps militaris spawn;
(4) bacterium is sent out:
Be placed in cultivating chamber by postvaccinal medium and cultivate, temperature 15-20 DEG C, humidity 60-80%, light culture 4-8 days until a bacterium is complete;
(5) fruit body development management:
Giving 100-300lux illumination completely when sending out bacterium, keeping temperature 15-20 DEG C, humidity 60-80%; Within 8-12 days, mycelia annesl is complete, within 14-20 days, can form stroma bud; During 40-50 days, introduce high temperature stress process, by temperature increase to 24-30 DEG C, keep 10-20 days;
(6) gather, dry
Within 55-65 days, gather; Dry when reaching below 10% to fruiting bodies of cordyceps militaris water content at 45-55 DEG C, package storage.
3. a kind of method improving cordycepin content in fruiting bodies of cordyceps militaris according to claim 1, it is characterized in that, within 40-50 days, rise while taking high temperature stress process, be aided with the strong White-Light processing of 1000-5000lux, cordycepin content in fruiting bodies of cordyceps militaris can be promoted further.
4. a kind of method improving cordycepin content in fruiting bodies of cordyceps militaris according to claim 1, it is characterized in that, within 40-50 days, rise while taking high temperature stress process, be aided with the strong blue light process of 1000-10000lux, cordycepin content in fruiting bodies of cordyceps militaris can be promoted further.
5. a kind of method improving cordycepin content in fruiting bodies of cordyceps militaris according to claim 1, it is characterized in that, in fruiting bodies of cordyceps militaris, cordycepin detection method is the NY/T2116-2012 mensuration high performance liquid chromatography of cordycepin and adenosine " in the cordyceps product ".
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Cited By (8)
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| CN106171529A (en) * | 2016-08-05 | 2016-12-07 | 吴剑翔 | The Cordyceps cultural method of worm summer herb with high-content of cordycepin and the culture medium of use thereof |
| CN106561264A (en) * | 2016-11-02 | 2017-04-19 | 仪征康海农业发展有限公司 | High-selenium high-cordycepin-content cordyceps militaris culturing method and culture medium thereof |
| CN109355211A (en) * | 2018-12-13 | 2019-02-19 | 蒋涛 | A kind of Moringa Cordyceps militaris and its cultural method and bacteriostasis spray |
| CN109691350A (en) * | 2017-10-20 | 2019-04-30 | 中国科学院微生物研究所 | The high-quality fructification production method of Cordyceps militaris that is a kind of while improving carotenoid and cordycepin content |
| CN111826410A (en) * | 2020-08-10 | 2020-10-27 | 辽东学院 | Method for obtaining high-yield cordycepin by using solid culture medium |
| CN114711086A (en) * | 2022-03-15 | 2022-07-08 | 江苏隆力奇生物科技股份有限公司 | Method for increasing cordycepin content in cordyceps militaris sporocarp by using wheat |
| CN115349397A (en) * | 2022-09-19 | 2022-11-18 | 山东创时代生物工程有限公司 | Method for increasing cordycepin content in cordyceps militaris sporocarp |
| CN115969889A (en) * | 2021-10-14 | 2023-04-18 | 光晟生物科技股份有限公司 | Application of cordyceps militaris sporocarp extract in preparing medicine for improving blue light injury resistance effect |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106561264A (en) * | 2016-11-02 | 2017-04-19 | 仪征康海农业发展有限公司 | High-selenium high-cordycepin-content cordyceps militaris culturing method and culture medium thereof |
| CN106561264B (en) * | 2016-11-02 | 2019-06-04 | 江苏康能生物工程股份有限公司 | A kind of cultural method and its culture medium of high selenium high cordycepin content Cordyceps militaris |
| CN109691350A (en) * | 2017-10-20 | 2019-04-30 | 中国科学院微生物研究所 | The high-quality fructification production method of Cordyceps militaris that is a kind of while improving carotenoid and cordycepin content |
| CN109355211A (en) * | 2018-12-13 | 2019-02-19 | 蒋涛 | A kind of Moringa Cordyceps militaris and its cultural method and bacteriostasis spray |
| CN111826410A (en) * | 2020-08-10 | 2020-10-27 | 辽东学院 | Method for obtaining high-yield cordycepin by using solid culture medium |
| CN115969889A (en) * | 2021-10-14 | 2023-04-18 | 光晟生物科技股份有限公司 | Application of cordyceps militaris sporocarp extract in preparing medicine for improving blue light injury resistance effect |
| CN115969889B (en) * | 2021-10-14 | 2024-05-31 | 光晟生物科技股份有限公司 | Application of Cordyceps militaris fruiting body extract in preparing medicine for improving blue light injury resistance effect |
| CN114711086A (en) * | 2022-03-15 | 2022-07-08 | 江苏隆力奇生物科技股份有限公司 | Method for increasing cordycepin content in cordyceps militaris sporocarp by using wheat |
| CN115349397A (en) * | 2022-09-19 | 2022-11-18 | 山东创时代生物工程有限公司 | Method for increasing cordycepin content in cordyceps militaris sporocarp |
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