CN115349397A - Method for increasing cordycepin content in cordyceps militaris sporocarp - Google Patents
Method for increasing cordycepin content in cordyceps militaris sporocarp Download PDFInfo
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- CN115349397A CN115349397A CN202211137188.5A CN202211137188A CN115349397A CN 115349397 A CN115349397 A CN 115349397A CN 202211137188 A CN202211137188 A CN 202211137188A CN 115349397 A CN115349397 A CN 115349397A
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 31
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 title claims abstract description 25
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 title claims abstract description 25
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 239000001963 growth medium Substances 0.000 claims abstract description 49
- 238000005286 illumination Methods 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 14
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 238000003306 harvesting Methods 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 8
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 14
- 235000007164 Oryza sativa Nutrition 0.000 claims description 9
- 235000009566 rice Nutrition 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 241000209140 Triticum Species 0.000 claims description 8
- 235000021307 Triticum Nutrition 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 239000001888 Peptone Substances 0.000 claims description 7
- 108010080698 Peptones Proteins 0.000 claims description 7
- 235000019319 peptone Nutrition 0.000 claims description 7
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 6
- 240000007594 Oryza sativa Species 0.000 claims 1
- 230000002708 enhancing effect Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- 241000209094 Oryza Species 0.000 description 8
- 241000190633 Cordyceps Species 0.000 description 5
- 239000002609 medium Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 241000239223 Arachnida Species 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 241000254173 Coleoptera Species 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000221775 Hypocreales Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 102000010909 Monoamine Oxidase Human genes 0.000 description 1
- 108010062431 Monoamine oxidase Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102100028255 Renin Human genes 0.000 description 1
- 108090000783 Renin Proteins 0.000 description 1
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000000621 bronchi Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides a method for improving cordycepin content in cordyceps militaris sporocarp, and relates to the technical field of artificial cultivation of cordyceps militaris. The method for improving the cordycepin content in the cordyceps militaris sporocarp comprises the following steps: the method comprises the following steps: preparing a culture medium: sealing the prepared culture medium, and placing the culture medium in a high-pressure moist heat sterilization pot for sterilization; step two: hypha culture: adjusting the temperature of the culture medium to 18-25 ℃, inoculating cordyceps militaris strains under aseptic condition, and culturing until spawn running is finished; step three: and (3) fruiting body culture: in 10-15 days of sporophore culture, raising the temperature of culture medium to 25-35 deg.C, and giving illumination of 1000-1200lx, maintaining humidity at 60-80%, after 15 days, adjusting the culture temperature of culture medium to 18-25 deg.C, and performing conventional culture until harvesting. The content of cordycepin can be obviously improved by increasing the temperature of the culture medium and enhancing the illumination intensity in the process of culturing the sporocarp.
Description
Technical Field
The invention relates to the technical field of artificial cultivation of cordyceps militaris, and particularly relates to a method for increasing the content of cordycepin in cordyceps militaris sporocarp.
Background
Cordyceps militaris, also known as Cordyceps militaris, belongs to Ascomycotina, pyrenomycetes, clavicipitales, cordyceps genus, clavicipitaceae, ascomycotina, and Cordyceps as one of Cordyceps fungi. Cordyceps fungi are a generic term for a class of entomogenous fungi complexes formed by parasitizing the underground fruiting bodies of insects, arachnids or macropolybia. Cordyceps militaris is a model species of cordyceps fungi, and is mainly hosted on larvae and pupae of some insects of Lepidoptera, coleoptera and Diptera in the field, and the efficacy of the cordyceps militaris is recorded by Chinese medical records as sweet taste, mild nature, benefit to lung and kidney, supplement essence, stop bleeding and reduce phlegm, and is basically consistent with the function of cordyceps militaris. In the past, because the output of the cordyceps militaris in the wild is rare, the development and utilization of the cordyceps militaris are not regarded by people. However, since the large-scale artificial culture of the cordyceps militaris has been successful in the end of the last ninety years, the development and utilization values of the cordyceps militaris are more and more known, more and more products are developed, the market demand is higher and more, and the artificial culture of the cordyceps militaris sporocarp is a new industry with great economic value.
Cordycepin is one of the main effective components in Cordyceps militaris, and is an adenosine active substance separated from Cuningham in 1951, and has molecular formula of C10H13N503, molecular weight of 251.24, and melting point of 230-231 deg.C. Cordycepin is one of main effective components in Cordyceps militaris, has antibacterial, antiviral, antitumor, antifatigue, antiaging, antiinflammatory, immunity enhancing, monoamine oxidase activity inhibiting, and high medicinal value and wide pharmacological action. The traditional Chinese medicine composition is mainly used for treating cancers and leukemia clinically, expanding bronchus, obviously enhancing renin, resisting aging and the like.
Through the search of patent documents, the 'cordyceps militaris culture method for improving cordycepin content' of patent No. CN201310541025.8 optimizes the formulas of a solid culture medium, a liquid culture medium and a fermentation liquid culture medium, improves the cordycepin content in cordyceps militaris fruiting bodies by using a high-concentration zinc ion stress mode, and the technical scheme has low improvement range, and the highest content in the preferred embodiment case is only 0.21%;
the application number CN201310217705.4 'cordyceps militaris culture method capable of improving cordycepin content', which adopts rice, black ant powder and the like as an optimized culture medium and realizes the improvement of cordycepin content in cordyceps militaris sporocarp by a day and night 10 ℃ temperature difference stimulation mode, but the black ant raw material in the technical scheme is in short supply, the quality of the black ant raw material cannot be effectively controlled, and the method is not suitable for large-scale production and application.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a method for improving the cordycepin content in cordyceps militaris sporocarp, and solves the problem that the cordycepin content level in cordyceps militaris sporocarp is generally low.
(II) technical scheme
In order to realize the purpose, the invention is realized by the following technical scheme: a method for increasing cordycepin content in Cordyceps militaris sporocarp comprises the following steps:
the method comprises the following steps: preparing a culture medium:
the weight ratio of each component is as follows: 30-40 parts of raw materials, 3-5 parts of glucose, 3-5 parts of peptone, 1-2 parts of sodium carbonate and a proper amount of water;
sealing the prepared culture medium, and placing the culture medium in a high-pressure moist heat sterilization pot for sterilization;
step two: hypha culture:
adjusting the temperature of the culture medium to 18-25 ℃, inoculating cordyceps militaris strains under aseptic conditions, controlling the temperature to be 18-25 ℃ in the daytime, giving 100-300lx illumination, keeping the humidity to be 60-70%, controlling the temperature to be 15-20 ℃ at night, giving 600-800lx illumination, keeping the humidity to be 60-80%, and culturing until spawn running is finished;
step three: and (3) fruiting body culture:
in 10-15 days of sporophore culture, raising the temperature of culture medium to 25-35 deg.C, and giving illumination of 1000-1200lx, maintaining humidity at 60-80%, after 15 days, adjusting the culture temperature of culture medium to 18-25 deg.C, and performing conventional culture until harvesting.
Preferably, in the first step, the raw material is one or a mixture of rice and wheat.
Preferably, in the first step, the sterilization conditions of the autoclave are as follows: 100-120 deg.C, 30-50min.
(III) advantageous effects
The invention provides a method for improving cordycepin content in cordyceps militaris sporocarp. The method has the following beneficial effects:
1. the content of cordycepin can be obviously improved by increasing the temperature of the culture medium and enhancing the illumination intensity in the process of culturing the fruiting body.
2. In the process of preparing the culture medium, rice and wheat are selected as the optimized culture medium and are matched with the action of glucose, so that on one hand, the materials are simple to obtain, on the other hand, the dosage is convenient to control, and the method is suitable for large-scale production and application.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
The first embodiment is as follows:
the embodiment of the invention provides a method for improving cordycepin content in cordyceps militaris sporocarp, which comprises the following steps:
the method comprises the following steps: preparing a culture medium:
the weight ratio of each component is as follows: 35 parts of rice and wheat mixture, 4 parts of glucose, 4 parts of peptone, 2 parts of sodium carbonate and a proper amount of water;
sealing the prepared culture medium, placing in a high-pressure moist heat sterilization pot, maintaining at 120 deg.C for 50min, and sterilizing;
step two: hypha culture:
adjusting the temperature of a culture medium to 18 ℃, inoculating cordyceps militaris strains under an aseptic condition, controlling the temperature to be 20 ℃ in the daytime, giving 200lx illumination, keeping the humidity to be 70%, controlling the temperature to be 15 ℃ at night, giving 600lx illumination, keeping the humidity to be 80%, and culturing until spawn running is finished;
step three: and (3) fruiting body culture:
in 10-15 days of the fruiting body culture, the culture medium temperature is raised to 25 deg.C and 1000lx of light is given, humidity is maintained at 80%, and after 15 days, the culture medium temperature is adjusted to 20 deg.C, and conventional culture is carried out until harvest.
Example two:
the embodiment of the invention provides a method for improving cordycepin content in cordyceps militaris sporocarp, which comprises the following steps:
the method comprises the following steps: preparing a culture medium:
the weight ratio of each component is as follows: 35 parts of rice and wheat mixture, 4 parts of glucose, 4 parts of peptone, 2 parts of sodium carbonate and a proper amount of water;
sealing the prepared culture medium, placing in a high-pressure moist heat sterilization pot, maintaining at 120 deg.C for 50min, and sterilizing;
step two: hypha culture:
adjusting the temperature of a culture medium to 18 ℃, inoculating cordyceps militaris strains under an aseptic condition, controlling the temperature to be 20 ℃ in the daytime, giving 200lx illumination, keeping the humidity to be 70%, controlling the temperature to be 15 ℃ at night, giving 600lx illumination, keeping the humidity to be 80%, and culturing until spawn running is finished;
step three: and (3) fruiting body culture:
in 10-15 days of the fruiting body culture, the culture medium temperature is raised to 30 ℃ and 1000lx of light is given, the humidity is kept at 80%, and after 15 days, the culture medium temperature is adjusted to 20 ℃ which is the normal culture temperature, and the normal culture is carried out until harvest.
Example three:
the embodiment of the invention provides a method for improving cordycepin content in cordyceps militaris sporocarp, which comprises the following steps:
the method comprises the following steps: preparing a culture medium:
the weight ratio of each component is as follows: 35 parts of rice and wheat mixture, 4 parts of glucose, 4 parts of peptone, 2 parts of sodium carbonate and a proper amount of water;
sealing the prepared culture medium, placing in a high-pressure moist heat sterilization pot, maintaining at 120 deg.C for 50min, and sterilizing;
step two: hypha culture:
adjusting the temperature of a culture medium to 18 ℃, inoculating cordyceps militaris strains under an aseptic condition, controlling the temperature to be 20 ℃ in the daytime, giving 200lx illumination, keeping the humidity to be 70%, controlling the temperature to be 15 ℃ at night, giving 600lx illumination, keeping the humidity to be 80%, and culturing until spawn running is finished;
step three: and (3) fruiting body culture:
in 10-15 days of the fruiting body culture, the culture medium temperature is raised to 35 deg.C, and 1000lx of light is given, humidity is maintained at 80%, and after 15 days, the culture medium temperature is adjusted to 20 deg.C, and conventional culture is carried out until harvest.
In summary, the first, second and third embodiments are different in that: for step three, the culture temperature after the medium was elevated was 25 ℃,30 ℃ and 35 ℃, respectively.
Example four:
the embodiment of the invention provides a method for improving cordycepin content in cordyceps militaris sporocarp, which comprises the following steps:
the method comprises the following steps: preparing a culture medium:
the weight ratio of each component is as follows: 35 parts of rice and wheat mixture, 4 parts of glucose, 4 parts of peptone, 2 parts of sodium carbonate and a proper amount of water;
sealing the prepared culture medium, placing in a high-pressure moist heat sterilization pot, maintaining at 120 deg.C for 50min, and sterilizing;
step two: hypha culture:
adjusting the temperature of a culture medium to 18 ℃, inoculating cordyceps militaris strains under an aseptic condition, controlling the temperature to be 20 ℃ in the daytime, giving 200lx illumination, keeping the humidity to be 70%, controlling the temperature to be 15 ℃ at night, giving 600lx illumination, keeping the humidity to be 80%, and culturing until spawn running is finished;
step three: and (3) fruiting body culture:
the culture medium temperature was raised to 35 ℃ during 10-15 days of fruiting body cultivation and 1200lx light was applied while maintaining humidity at 80%, and after 15 days, the culture medium temperature was adjusted to 20 ℃ as the conventional culture temperature, and conventional cultivation was performed until harvest.
In summary, the difference between the third embodiment and the fourth embodiment is: the light intensity given to the medium was 1000lx and 1200lx, respectively.
Comparative example:
the embodiment of the invention provides a method for improving cordycepin content in cordyceps militaris sporocarp, which comprises the following steps:
the method comprises the following steps: preparing a culture medium:
the weight ratio of each component is as follows: 35 parts of rice and wheat mixture, 4 parts of glucose, 4 parts of peptone, 2 parts of sodium carbonate and a proper amount of water;
sealing the prepared culture medium, placing in a high-pressure moist heat sterilization pot, maintaining at 120 deg.C for 50min, and sterilizing;
step two: hypha culture:
adjusting the temperature of a culture medium to 18 ℃, inoculating cordyceps militaris strains under an aseptic condition, controlling the temperature to be 20 ℃ in the daytime, giving 200lx of illumination, keeping the humidity to be 70%, controlling the temperature to be 15 ℃ at night, giving 600lx of illumination, keeping the humidity to be 80%, and culturing until spawn running is finished;
step three: and (3) fruiting body culture:
the culture temperature of the medium was adjusted to 20 ℃ as the conventional culture temperature, and the conventional culture was carried out until harvest.
In summary, the comparative examples differ from examples one, two, three and four in that: no treatment was made on the medium during 10-15 days of fruiting body culture.
The results of the cultivation in the above examples are tabulated as follows:
although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. A method for increasing cordycepin content in cordyceps militaris sporocarp is characterized by comprising the following steps:
the method comprises the following steps: preparing a culture medium:
the weight ratio of each component is as follows: 30-40 parts of raw materials, 3-5 parts of glucose, 3-5 parts of peptone, 1-2 parts of sodium carbonate and a proper amount of water;
sealing the prepared culture medium, and placing the culture medium in a high-pressure moist heat sterilization pot for sterilization;
step two: hypha culture:
adjusting the temperature of the culture medium to 18-25 ℃, inoculating cordyceps militaris strains under aseptic conditions, controlling the temperature to be 18-25 ℃ in the daytime, giving 100-300lx illumination, keeping the humidity to be 60-70%, controlling the temperature to be 15-20 ℃ at night, giving 600-800lx illumination, keeping the humidity to be 60-80%, and culturing until spawn running is completed;
step three: and (3) fruiting body culture:
in 10-15 days of sporophore culture, raising the temperature of culture medium to 25-35 deg.C, and giving illumination of 1000-1200lx, maintaining humidity at 60-80%, after 15 days, adjusting the culture temperature of culture medium to 18-25 deg.C, and performing conventional culture until harvesting.
2. The method for increasing the content of cordycepin in the fruiting body of cordyceps militaris according to claim 1, wherein the method comprises the following steps: in the first step, the raw material is one or a mixture of two of rice and wheat.
3. The method for increasing the content of cordycepin in cordyceps militaris sporocarp according to claim 1, which is characterized by comprising the following steps: in the first step, the sterilization conditions of the high-pressure moist heat sterilization pot are as follows: 100-120 deg.C, 30-50min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211137188.5A CN115349397A (en) | 2022-09-19 | 2022-09-19 | Method for increasing cordycepin content in cordyceps militaris sporocarp |
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| CN202211137188.5A CN115349397A (en) | 2022-09-19 | 2022-09-19 | Method for increasing cordycepin content in cordyceps militaris sporocarp |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598006A (en) * | 2013-10-24 | 2014-02-26 | 上海市农业科学院 | Method for increasing content of cordycepin in cordyceps militaris links |
| CN104756763A (en) * | 2015-04-16 | 2015-07-08 | 刘晓红 | Method for improving content of cordycepin in cordyceps militaris fruiting bodies |
| CN105248150A (en) * | 2015-11-19 | 2016-01-20 | 江苏康能生物工程股份有限公司 | Method for increasing content of cordycepin in cordyceps militaris sporocarp |
| CN108207484A (en) * | 2016-12-22 | 2018-06-29 | 志升（上海）医药科技有限公司 | A kind of breeding method of silkworm chrysalis cordyceps sinensis |
-
2022
- 2022-09-19 CN CN202211137188.5A patent/CN115349397A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598006A (en) * | 2013-10-24 | 2014-02-26 | 上海市农业科学院 | Method for increasing content of cordycepin in cordyceps militaris links |
| CN104756763A (en) * | 2015-04-16 | 2015-07-08 | 刘晓红 | Method for improving content of cordycepin in cordyceps militaris fruiting bodies |
| CN105248150A (en) * | 2015-11-19 | 2016-01-20 | 江苏康能生物工程股份有限公司 | Method for increasing content of cordycepin in cordyceps militaris sporocarp |
| CN108207484A (en) * | 2016-12-22 | 2018-06-29 | 志升（上海）医药科技有限公司 | A kind of breeding method of silkworm chrysalis cordyceps sinensis |
Non-Patent Citations (1)
| Title |
|---|
| 王贺祥等: "《中国液体菌种生产新技术》", vol. 1, 中国农业大学出版社, pages: 268 * |
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