CN108207484A - A kind of breeding method of silkworm chrysalis cordyceps sinensis - Google Patents
A kind of breeding method of silkworm chrysalis cordyceps sinensis Download PDFInfo
- Publication number
- CN108207484A CN108207484A CN201611224032.5A CN201611224032A CN108207484A CN 108207484 A CN108207484 A CN 108207484A CN 201611224032 A CN201611224032 A CN 201611224032A CN 108207484 A CN108207484 A CN 108207484A
- Authority
- CN
- China
- Prior art keywords
- silkworm chrysalis
- cordyceps sinensis
- temperature
- breeding method
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 39
- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 32
- 238000009395 breeding Methods 0.000 title claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 25
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 241001264174 Cordyceps militaris Species 0.000 claims abstract description 11
- 238000003306 harvesting Methods 0.000 claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 238000005286 illumination Methods 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 24
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 9
- 235000004237 Crocus Nutrition 0.000 claims description 6
- 241000596148 Crocus Species 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 230000000740 bleeding effect Effects 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- LGZXYFMMLRYXLK-UHFFFAOYSA-N mercury(2+);sulfide Chemical compound [S-2].[Hg+2] LGZXYFMMLRYXLK-UHFFFAOYSA-N 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 6
- 239000010935 stainless steel Substances 0.000 claims description 6
- 230000000638 stimulation Effects 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 238000012549 training Methods 0.000 claims description 2
- 238000011068 loading method Methods 0.000 claims 1
- 238000005057 refrigeration Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 230000036541 health Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 241000233866 Fungi Species 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 240000001307 Myosotis scorpioides Species 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 238000001035 drying Methods 0.000 abstract description 2
- 238000007710 freezing Methods 0.000 abstract description 2
- 230000008014 freezing Effects 0.000 abstract description 2
- 235000013402 health food Nutrition 0.000 abstract description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 abstract 1
- 241001625808 Trona Species 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 230000035911 sexual health Effects 0.000 abstract 1
- 241000190633 Cordyceps Species 0.000 description 9
- 230000032258 transport Effects 0.000 description 5
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 4
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 4
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 4
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 241000382353 Pupa Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000004224 protection Effects 0.000 description 2
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to Cordyceps militaris breeding method technical fields, and in particular to a kind of breeding method of silkworm chrysalis cordyceps sinensis includes the following steps：1) prepared by culture medium；2) it sterilizes；3) it is inoculated with；4) it manages：Mycelial growth, fructification rudiment, sporophore growth；5) it harvests.The silkworm chrysalis cordyceps sinensis of the cultivation of the present invention, entity is full, color is golden yellow, its identified main nutrient composition is higher than wild Chinese caterpillar fungus, by the low temperature rapid freezing sterilizing immediately of the cordyceps militaris sporocarp after harvesting and drying, its all fresh Nutrient composition reservation is made not to be lost in, and the plant enzyme of different attribute is injected in cultivating process, it obtains stressing the different five-element's silkworm chrysalis Cordyceps sinensis fructification of effect, so as to which various trona sexual health food be made, the effect of reaching health, health care, prevention to treatment, is to manufacture related drug, the desirable feedstock of health products.
Description
Technical field
The present invention relates to Cordyceps militaris breeding method technical fields, and in particular to a kind of breeding method of silkworm chrysalis cordyceps sinensis.
Background technology
The wild cordyceps of scarcity of resources are as traditional precious Chinese medicine and invigorant, and supply falls short of demand always in market.Profit
The silkworm chrysalis cordyceps sinensis cultivated with silkworm pupa is the medicinal fungi that silkworm and Xizang Wild bacterium combine, and is under the jurisdiction of the son of mycota
Capsule bacterium subphylum, Clavicipitaceae, Cordyceps.Silkworm chrysalis cordyceps sinensis is not only containing abundant protein and amino acid, cordycepin, cordyceps sinensis
Acid, Cordyceps sinensis polysaccharide, and containing the trace element of more than 30 kinds of needed by human body, a variety of physiologically active ingredients contained have anti-swollen
The pharmacological actions such as knurl, antibacterial, anti-oxidant, protection liver, strengthen immunity.Both at home and abroad cordyceps militaris sporocarp is induced to having become
Research on kinds thinks that silkworm chrysalis cordyceps sinensis is eaten can match in excellence or beauty with medical value with traditional cordyceps sinensis, and biotechnology is manually trained
Foster silkworm chrysalis cordyceps sinensis cellulose content is higher than traditional tens times of wild cordyceps, is that wild cordyceps are important, ideal
Substitute is more and more favored by market.However, silkworm chrysalis cordyceps sinensis one kind be biotechnology artificial growth with the raw bacterium of worm pupa and
Into plantation difficulty is big, survival rate is low, and the nutritive effect component content of silkworm chrysalis winter worm summer vegetable need to be further improved.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of breeding methods of silkworm chrysalis cordyceps sinensis.
In order to achieve the above object, the present invention is achieved by the following technical programs：
A kind of breeding method of silkworm chrysalis cordyceps sinensis, includes the following steps：
1) prepared by culture medium：High-quality green silkworm chrysalis is selected, is immersed in culture solution after 30-40min and is packed into glass training
It supports in bottle, obtains culture medium；
The formula rate of the culture solution is：Glucose 10-15g, peptone 4-7g, potassium dihydrogen phosphate 2-4g, magnesium sulfate
0.5-2g adds water constant volume to 1000mL；
2) it sterilizes：Culture bottle sealing is put into high-pressure sterilizing pot and is sterilized, is warming up to 100 DEG C, closes air bleeding valve, when
High-pressure sterilizing pot pressure keeps 1h when reaching 1.5bar, take out, cooling；
3) it is inoculated with：Aseptically, liquid spawn is sprayed with inoculating gun even in media surface；
4) it manages：
1. mycelial growth：The culture bottle for accessing liquid spawn is moved into culturing room and is protected from light culture, temperature is controlled in 15-18
DEG C, temperature when mycelia covers with media surface 1/2-2/3, is risen to 19-22 DEG C by humidity 75-85%, until mycelia is covered with
Media surface；
2. fructification rudiment：When mycelia protrusion occurs in culture medium top, control day temperature is in 22-25 DEG C, humidity
80-85%, evening temperature are 12-15 DEG C, give 10-15 DEG C of thermal stimulation, and daylight is protected from light at night, and intensity of illumination is
200-250Lx, light application time are no less than 12h；
3. sporophore growth：When fructification length is to 1cm, culture bottle cap is opened, day temperature is controlled at 18-22 DEG C,
Humidity is 80-90%, and evening temperature is controlled at 15 DEG C -18 DEG C, and it is 5 DEG C -7 DEG C to make temperature difference, and daylight is protected from light at night, illumination
Intensity is 300-400Lx, and light application time is no less than 10h；When fruiting bodies of cordyceps militaris growing height reaches more than 3cm, increase light
According to slightly to 600-800Lx, throughout the growing season injecting ferment into culture bottle every 5-7 days.
5) it harvests：When fructification stroma is rodlike in Chinese red or crocus and no longer grows, stainless steel pocket knife or scissors
It from base portion is adopted down, cleaner is put into and enters freeze-drying, be stored in sealed plastic bag after dry, it is cold to transport freezer
It hides and preserves.
Preferably, the silkworm chrysalis place of production is the pollution-free area of Rizhao inshore.
Preferably, the mass ratio of liquid spawn and culture medium is 1: 40-50 in the step 3).
Preferably, the density of liquid spawn is 3-5 bacterium ball/mL in the step 3).
Preferably, the preparation method of ferment is in the step 4)：Using medicinal plant and fruit after fermentation in 2 years, warp
It is obtained after 3-4 concentration extraction.
Preferably, freeze-drying temperature is -40 DEG C in the step 5), and refrigerated storage temperature is -22 DEG C.
Advantageous effect of the present invention：The present invention cultivation silkworm chrysalis cordyceps sinensis, entity is full, and color is golden yellow, it is identified its
Main nutrient composition is higher than 12 times of wild Chinese caterpillar fungus, the wherein about traditional wild cordyceps of cordycepin content, and cordycepic acid is up to
Its 1750 times, and SOD isoreactivities ingredient, far above traditional wild cordyceps, the present invention founds the cordyceps militaris sporocarp after harvesting
That is the sterilizing of low temperature rapid freezing and drying make its all fresh Nutrient compositions reservation not to be lost in, are injected not in cultivating process
It with the plant enzyme of attribute, obtains stressing the different five-element's silkworm chrysalis Cordyceps sinensis fructification of effect, be protected so as to which various natural alkalinity be made
Health food is to manufacture related drug, the desirable feedstock of health products the effect of reaching health, health care, prevent extremely to treat.
Specific embodiment
Purpose, technical scheme and advantage to make the embodiment of the present invention are clearer, below in conjunction with the embodiment of the present invention,
Technical solution in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is the present invention one
Divide embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making
All other embodiments obtained under the premise of creative work, shall fall within the protection scope of the present invention.
Embodiment 1：
A kind of breeding method of silkworm chrysalis cordyceps sinensis, includes the following steps：
1) prepared by culture medium：The high-quality green silkworm chrysalis in the pollution-free area of Rizhao inshore is selected, is immersed in culture solution
It is fitted into after 40min in glass culture bottle, obtains culture medium；
The formula rate of the culture solution is：Glucose 12g, peptone 5g, potassium dihydrogen phosphate 3g, magnesium sulfate 1g, add water
Constant volume is to 1000mL；
2) it sterilizes：Culture bottle sealing is put into high-pressure sterilizing pot and is sterilized, is warming up to 100 DEG C, closes air bleeding valve, when
High-pressure sterilizing pot pressure keeps 1h when reaching 1.5bar, take out, cooling；
3) it is inoculated with：Aseptically, liquid spawn is sprayed with inoculating gun even in media surface, the density of liquid spawn
For 5 bacterium ball/mL, the mass ratio of liquid spawn and culture medium is 1: 45；
4) it manages：
1. mycelial growth：The culture bottle for accessing liquid spawn is moved into culturing room and is protected from light culture, temperature is controlled at 16 DEG C,
Humidity is 80%, and when mycelia covers with media surface 1/2, temperature is risen to 20 DEG C, until mycelia covers with media surface；
2. fructification rudiment：When mycelia protrusion occurs in culture medium top, control day temperature is in 24 DEG C, humidity
82%, evening temperature is 14 DEG C, gives 10 DEG C of thermal stimulation, and daylight is protected from light at night, intensity of illumination 240Lx, illumination
Time is no less than 12h；
3. sporophore growth：When fructification length is to 1cm, culture bottle cap is opened, day temperature control is at 20 DEG C, humidity
It is 85%, evening temperature is controlled at 15 DEG C, and it is 5 DEG C to make temperature difference, and daylight is protected from light at night, intensity of illumination 350Lx, illumination
Time is no less than 10h；When fruiting bodies of cordyceps militaris growing height reaches more than 3cm, increase illumination slightly to 700Lx, it is entire raw
Injected ferment during length into culture bottle every 6 days, the preparation method of ferment is：It was fermented using medicinal plant and fruit through 2 years
Afterwards, it is obtained after 4 concentration extractions.
5) it harvests：When fructification stroma is rodlike in Chinese red or crocus and no longer grows, stainless steel pocket knife or scissors
It from base portion is adopted down, cleaner is put into and is freeze-dried at -40 DEG C, is stored in sealed plastic bag, transports cold after dry
Zang Ku -22 DEG C stored refrigerated.
Embodiment 2：
A kind of breeding method of silkworm chrysalis cordyceps sinensis, includes the following steps：
1) prepared by culture medium：The high-quality green silkworm chrysalis in the pollution-free area of Rizhao inshore is selected, is immersed in culture solution
It is fitted into after 30min in glass culture bottle, obtains culture medium；
The formula rate of the culture solution is：Glucose 15g, peptone 4g, potassium dihydrogen phosphate 4g, magnesium sulfate 0.5g, add
Water constant volume is to 1000mL；
2) it sterilizes：Culture bottle sealing is put into high-pressure sterilizing pot and is sterilized, is warming up to 100 DEG C, closes air bleeding valve, when
High-pressure sterilizing pot pressure keeps 1h when reaching 1.5bar, take out, cooling；
3) it is inoculated with：Aseptically, liquid spawn is sprayed with inoculating gun even in media surface, the density of liquid spawn
For 3 bacterium ball/mL, the mass ratio of liquid spawn and culture medium is 1: 40；
4) it manages：
1. mycelial growth：The culture bottle for accessing liquid spawn is moved into culturing room and is protected from light culture, temperature is controlled at 15 DEG C,
Humidity is 85%, and when mycelia covers with media surface 1/2, temperature is risen to 19 DEG C, until mycelia covers with media surface；
2. fructification rudiment：When mycelia protrusion occurs in culture medium top, control day temperature is in 22 DEG C, humidity
85%, evening temperature is 12 DEG C, gives 10 DEG C of thermal stimulation, and daylight is protected from light at night, intensity of illumination 200Lx, illumination
Time is no less than 12h；
3. sporophore growth：When fructification length is to 1cm, culture bottle cap is opened, day temperature control is at 22 DEG C, humidity
It is 90%, evening temperature is controlled at 15 DEG C, and it is 7 DEG C to make temperature difference, and daylight is protected from light at night, intensity of illumination 400Lx, illumination
Time is no less than 10h；When fruiting bodies of cordyceps militaris growing height reaches more than 3cm, increase illumination slightly to 800Lx, it is entire raw
Injected ferment during length into culture bottle every 5 days, the preparation method of ferment is：It was fermented using medicinal plant and fruit through 2 years
Afterwards, it is obtained after 3 concentration extractions.
5) it harvests：When fructification stroma is rodlike in Chinese red or crocus and no longer grows, stainless steel pocket knife or scissors
It from base portion is adopted down, cleaner is put into and is freeze-dried at -40 DEG C, is stored in sealed plastic bag, transports cold after dry
Zang Ku -22 DEG C stored refrigerated.
Embodiment 3：
A kind of breeding method of silkworm chrysalis cordyceps sinensis, includes the following steps：
1) prepared by culture medium：The high-quality green silkworm chrysalis in the pollution-free area of Rizhao inshore is selected, is immersed in culture solution
It is fitted into after 40min in glass culture bottle, obtains culture medium；
The formula rate of the culture solution is：Glucose 10g, peptone 7g, potassium dihydrogen phosphate 2g, magnesium sulfate 2g, add water
Constant volume is to 1000mL；
2) it sterilizes：Culture bottle sealing is put into high-pressure sterilizing pot and is sterilized, is warming up to 100 DEG C, closes air bleeding valve, when
High-pressure sterilizing pot pressure keeps 1h when reaching 1.5bar, take out, cooling；
3) it is inoculated with：Aseptically, liquid spawn is sprayed with inoculating gun even in media surface, the density of liquid spawn
For 4 bacterium ball/mL, the mass ratio of liquid spawn and culture medium is 1: 50；
4) it manages：
1. mycelial growth：The culture bottle for accessing liquid spawn is moved into culturing room and is protected from light culture, temperature is controlled at 18 DEG C,
Humidity is 75%, and when mycelia covers with media surface 2/3, temperature is risen to 22 DEG C, until mycelia covers with media surface；
2. fructification rudiment：When mycelia protrusion occurs in culture medium top, control day temperature is in 25 DEG C, humidity
80%, evening temperature is 12 DEG C, gives 13 DEG C of thermal stimulation, and daylight is protected from light at night, intensity of illumination 250Lx, illumination
Time is no less than 12h；
3. sporophore growth：When fructification length is to 1cm, culture bottle cap is opened, day temperature control is at 21 DEG C, humidity
It is 80%, evening temperature is controlled at 15 DEG C, and it is 6 DEG C to make temperature difference, and daylight is protected from light at night, intensity of illumination 300Lx, illumination
Time is no less than 10h；When fruiting bodies of cordyceps militaris growing height reaches more than 3cm, increase illumination slightly to 600Lx, it is entire raw
Injected ferment during length into culture bottle every 6 days, the preparation method of ferment is：It was fermented using medicinal plant and fruit through 2 years
Afterwards, it is obtained after 4 concentration extractions.
5) it harvests：When fructification stroma is rodlike in Chinese red or crocus and no longer grows, stainless steel pocket knife or scissors
It from base portion is adopted down, cleaner is put into and is freeze-dried at -40 DEG C, is stored in sealed plastic bag, transports cold after dry
Zang Ku -22 DEG C stored refrigerated.
Embodiment 4：
A kind of breeding method of silkworm chrysalis cordyceps sinensis, includes the following steps：
1) prepared by culture medium：The high-quality green silkworm chrysalis in the pollution-free area of Rizhao inshore is selected, is immersed in culture solution
It is fitted into after 35min in glass culture bottle, obtains culture medium；
The formula rate of the culture solution is：Glucose 14g, peptone 6g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, add
Water constant volume is to 1000mL；
2) it sterilizes：Culture bottle sealing is put into high-pressure sterilizing pot and is sterilized, is warming up to 100 DEG C, closes air bleeding valve, when
High-pressure sterilizing pot pressure keeps 1h when reaching 1.5bar, take out, cooling；
3) it is inoculated with：Aseptically, liquid spawn is sprayed with inoculating gun even in media surface, the density of liquid spawn
For 4 bacterium ball/mL, the mass ratio of liquid spawn and culture medium is 1: 40；
4) it manages：
1. mycelial growth：The culture bottle for accessing liquid spawn is moved into culturing room and is protected from light culture, temperature is controlled at 16 DEG C,
Humidity is 75%, and when mycelia covers with media surface 2/3, temperature is risen to 20 DEG C, until mycelia covers with media surface；
2. fructification rudiment：When mycelia protrusion occurs in culture medium top, control day temperature is in 24 DEG C, humidity
85%, evening temperature is 14 DEG C, gives 10 DEG C of thermal stimulation, and daylight is protected from light at night, intensity of illumination 250Lx, illumination
Time is no less than 12h；
3. sporophore growth：When fructification length is to 1cm, culture bottle cap is opened, day temperature control is at 20 DEG C, humidity
It is 80%, evening temperature is controlled at 15 DEG C, and it is 5 DEG C to make temperature difference, and daylight is protected from light at night, intensity of illumination 400Lx, illumination
Time is no less than 10h；When fruiting bodies of cordyceps militaris growing height reaches more than 3cm, increase illumination slightly to 650Lx, it is entire raw
Injected ferment during length into culture bottle every 6 days, the preparation method of ferment is：It was fermented using medicinal plant and fruit through 2 years
Afterwards, it is obtained after 4 concentration extractions.
5) it harvests：When fructification stroma is rodlike in Chinese red or crocus and no longer grows, stainless steel pocket knife or scissors
It from base portion is adopted down, cleaner is put into and is freeze-dried at -40 DEG C, is stored in sealed plastic bag, transports cold after dry
Zang Ku -22 DEG C stored refrigerated.
Silkworm chrysalis cordyceps sporophore prepared by the embodiment of the present invention 1 and commercially available Yushu district, Qinghai wild cordyceps
Entity warp let-off Chinese Academy of Sciences Shenyang ecology institute's agricultural product security is detected with environmental quality inspection center, and testing result is shown in Table 1.
The nutritional ingredient of 1 silkworm chrysalis cordyceps sinensis of table and Qinghai Wild cordyceps sinensis compares
From table 1 it follows that the silkworm chrysalis cordyceps sinensis that embodiment 1 is cultivated is rich in battalion such as amino acid, cordycepin, cordycepic acids
It forms point, and the content of the physiologically active ingredients such as cordycepic acid and cordycepin will far be more than wild cordyceps, the embodiment of the present invention
The cordyceps sinensis of cultivation have it is antitumor, keep fit and healthy, anti-bacteria and anti-virus, immunological regulation, remove a variety of pharmacology such as free radical and
Health-care efficacy.
It should be noted that herein, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any this practical relationship or sequence.Moreover, term " comprising ", "comprising" or its any other variant are intended to
Non-exclusive inclusion, so that process, method, article or equipment including a series of elements not only will including those
Element, but also including other elements that are not explicitly listed or further include as this process, method, article or equipment
Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
Also there are other identical elements in process, method, article or equipment including the element.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
The present invention is described in detail, it will be understood by those of ordinary skill in the art that：It still can be to aforementioned each implementation
Technical solution recorded in example modifies or carries out equivalent replacement to which part technical characteristic；And these modification or
It replaces, the spirit and scope for various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution.
Claims (6)
1. a kind of breeding method of silkworm chrysalis cordyceps sinensis, which is characterized in that include the following steps：
1) prepared by culture medium：High-quality green silkworm chrysalis is selected, is immersed in culture solution loading glass culture bottle after 30-40min
In, obtain culture medium；
The formula rate of the culture solution is：Glucose 10-15g, peptone 4-7g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 0.5-
2g adds water constant volume to 1000mL；
2) it sterilizes：Culture bottle sealing is put into high-pressure sterilizing pot and is sterilized, is warming up to 100 DEG C, air bleeding valve is closed, works as high pressure
Autoclave pressure keeps 1h when reaching 1.5bar, take out, cooling；
3) it is inoculated with：Aseptically, liquid spawn is sprayed with inoculating gun even in media surface；
4) it manages：
1. mycelial growth：The culture bottle for accessing liquid spawn is moved into culturing room and is protected from light culture, temperature is controlled at 15-18 DEG C,
Humidity is 75-85%, and when mycelia covers with media surface 1/2-2/3, temperature is risen to 19-22 DEG C, until mycelia covers with training
Support primary surface；
2. fructification rudiment：When mycelia protrusion occurs in culture medium top, control day temperature is at 22-25 DEG C, humidity 80-
85%, evening temperature is 12-15 DEG C, gives 10-15 DEG C of thermal stimulation, and daylight is protected from light at night, intensity of illumination 200-
250Lx, light application time are no less than 12h；
3. sporophore growth：When fructification length is to 1cm, culture bottle cap is opened, day temperature control is at 18-22 DEG C, humidity
For 80-90%, evening temperature is controlled at 15 DEG C -18 DEG C, and it is 5 DEG C -7 DEG C to make temperature difference, and daylight is protected from light at night, intensity of illumination
For 300-400Lx, light application time is no less than 10h；When fruiting bodies of cordyceps militaris growing height reaches more than 3cm, it is light to increase illumination
Degree throughout the growing season injected ferment into culture bottle to 600-800Lx every 5-7 days.
5) it harvests：When fructification stroma is rodlike in Chinese red or crocus and when no longer growing, stainless steel pocket knife or scissors by its
It is adopted down from base portion, is put into cleaner and enters freeze-drying, be stored in sealed plastic bag after dry, transported freezer refrigeration and protect
It deposits.
2. the breeding method of silkworm chrysalis cordyceps sinensis as described in claim 1, which is characterized in that the silkworm chrysalis place of production is day Zhaohai
Shore is pollution-free area.
3. the breeding method of silkworm chrysalis cordyceps sinensis as described in claim 1, which is characterized in that liquid spawn in the step 3)
Mass ratio with culture medium is 1: 40-50.
4. the breeding method of silkworm chrysalis cordyceps sinensis as described in claim 1, which is characterized in that liquid spawn in the step 3)
Density be 3-5 bacterium ball/mL.
5. the breeding method of silkworm chrysalis cordyceps sinensis as described in claim 1, which is characterized in that the system of ferment in the step 4)
Preparation Method is：Using medicinal plant and fruit after fermentation in 2 years, obtained after 3-4 concentration extraction.
6. the breeding method of silkworm chrysalis cordyceps sinensis as described in claim 1, which is characterized in that be freeze-dried in the step 5)
Temperature is -40 DEG C, and refrigerated storage temperature is -22 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611224032.5A CN108207484A (en) | 2016-12-22 | 2016-12-22 | A kind of breeding method of silkworm chrysalis cordyceps sinensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611224032.5A CN108207484A (en) | 2016-12-22 | 2016-12-22 | A kind of breeding method of silkworm chrysalis cordyceps sinensis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108207484A true CN108207484A (en) | 2018-06-29 |
Family
ID=62657232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611224032.5A Pending CN108207484A (en) | 2016-12-22 | 2016-12-22 | A kind of breeding method of silkworm chrysalis cordyceps sinensis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108207484A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109348983A (en) * | 2018-09-12 | 2019-02-19 | 泰安皇草生物医药科技有限公司 | A kind of breeding method of cordyceps sinensis |
| CN112753933A (en) * | 2020-12-30 | 2021-05-07 | 天津天狮学院 | Functional reconstituted rice flour based on waste cordyceps militaris culture medium and preparation method thereof |
| CN115349397A (en) * | 2022-09-19 | 2022-11-18 | 山东创时代生物工程有限公司 | Method for increasing cordycepin content in cordyceps militaris sporocarp |
-
2016
- 2016-12-22 CN CN201611224032.5A patent/CN108207484A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109348983A (en) * | 2018-09-12 | 2019-02-19 | 泰安皇草生物医药科技有限公司 | A kind of breeding method of cordyceps sinensis |
| CN112753933A (en) * | 2020-12-30 | 2021-05-07 | 天津天狮学院 | Functional reconstituted rice flour based on waste cordyceps militaris culture medium and preparation method thereof |
| CN115349397A (en) * | 2022-09-19 | 2022-11-18 | 山东创时代生物工程有限公司 | Method for increasing cordycepin content in cordyceps militaris sporocarp |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101720627A (en) | Method for culturing cordyceps militaris by living silkworm chrysalises | |
| CN103416225A (en) | Method for preparing high-quality ganoderma lucidum compost | |
| CN104798601A (en) | Cultivation method for lentinula edodes | |
| CN103843583A (en) | Industrialized production method of green cordyceps militaris | |
| CN104956913A (en) | Method for cultivating artificial natural ganoderma lucidum under high-altitude forest | |
| CN104871830B (en) | With bean worm as the method for carrier-planting Cordyceps militaris (L.) Link. | |
| CN104419602A (en) | Cordyceps sinensis liquor, preparation and application thereof | |
| CN104350942A (en) | Method for preparing cordyceps mycelia through mulberry twig powder and silkworm chrysalis powder | |
| CN106797804A (en) | A kind of method that silkworm using abnormal early stage cultivates Cordyceps militaris | |
| CN104012303B (en) | The breeding method of Pleurotus geesteranus edible fungi | |
| CN107493965A (en) | A kind of implantation methods for improving white fungus protein content | |
| CN108207484A (en) | A kind of breeding method of silkworm chrysalis cordyceps sinensis | |
| CN109234171B (en) | Preparation method of selenium-rich ganoderma lucidum mycelium powder | |
| CN104303835A (en) | Cultivation method of cordyceps sinensis | |
| CN107125018A (en) | A kind of packed breeding method of golden ear | |
| KR101634587B1 (en) | Cultivation method of termitomyces | |
| KR100876820B1 (en) | A cultivating method for cordyceps sp | |
| CN103688744B (en) | A kind of Cordceps militaris breeding method and using method | |
| TWI472614B (en) | Method for cultivation of cordyceps militaris using supercritical fluid extraction of brown rice | |
| CN103695267B (en) | Preparation method of living cicada pupa-inoculated growing cordyceps militaris medicinal liquor | |
| CN110122175B (en) | Planting method of lucid ganoderma | |
| CN104396576B (en) | Cultivation method of factory-like bottle-cultivated Tricholoma giganteum | |
| CN107182555A (en) | A kind of chestnut mushroom and the interplanting method of agaric | |
| KR20210153478A (en) | Mushroom Cultivation Method Using Nutrient Containing Metabolite of Lactobacillus Fermented at Room Temperature and Concentrated Mineral Water from Seawater | |
| CN105154296A (en) | Preparation method of cordyceps wine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180619 Address after: 200437 Room 502, No. 29, Wenchuan Road, Hongkou District, Shanghai Applicant after: Tai'an Huangcao bio medicine & Technology Co., Ltd. Address before: 200439 room 218, building A, 180 South Changjiang Road, Baoshan District, Shanghai. Applicant before: Zhi Sheng (Shanghai) Pharmaceutical Technology Co., Ltd. |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhi Sheng (Shanghai) Pharmaceutical Technology Co., Ltd. Document name: Notification of Passing Examination on Formalities |
|
| DD01 | Delivery of document by public notice |
Addressee: Tai'an Huangcao bio medicine & Technology Co., Ltd. Document name: Notice of payment reduction |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Tai'an Huangcao bio medicine & Technology Co., Ltd. Document name: Notification of before Expiration of Request of Examination as to Substance |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: TAI'AN HUANGCAO BIOMEDICAL TECHNOLOGY Co.,Ltd. Document name: Notification that Application Deemed to be Withdrawn |
|
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180629 |