CN107853077A - A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum - Google Patents
A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum Download PDFInfo
- Publication number
- CN107853077A CN107853077A CN201711268568.1A CN201711268568A CN107853077A CN 107853077 A CN107853077 A CN 107853077A CN 201711268568 A CN201711268568 A CN 201711268568A CN 107853077 A CN107853077 A CN 107853077A
- Authority
- CN
- China
- Prior art keywords
- parts
- rich
- solid
- lucid ganoderma
- selenium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
Abstract
This application provides a kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum, the solid Se-rich lucid ganoderma culture medium to be made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；The weight proportion of the raw material is：55 65 parts of 25 35 parts of selenium-rich Juncao, 70 80 parts of wood chip, 20 30 parts of wheat bran, 10 20 parts of peanut shell, 2 12 parts of land plaster, 1 11 parts of precipitated calcium carbonate, 10 20 parts of maltose, 2 12 parts of protein peptides and water.Solves the problem of selenium element content is not high, and ganoderma lucidum yields poorly and biological transformation ratio is low in the ganoderma lucidum of conventional art production.
Description
Technical field
The application is related to fungus growing technique field, especially actual Se-rich lucid ganoderma culture medium and its side for producing ganoderma lucidum
Method.
Background technology
Ganoderma lucidum is also known as polyporus lucidus, refreshing sesame, sesame grass, celestial grass or seocho.Ganoderma lucidum profile is in umbrella, cap kidney shape, it is semicircle or
Subcircular, it is the complete stool of Polyporaceae plant red sesame or purple sesame.Using Ganoderma Sinense drug effect to be best, ganoderma lucidum originates in east Asia,
Chinese branch is most wide to be possessed very high in Jiangxi, ganoderma lucidum as the Chinese tradition valuable ingredient of traditional Chinese medicine for possessing thousands of years of medicinal histories
Medical value, ganoderma lucidum contain polysaccharide, several amino acids, organic germanium, steroid and triterpenes in fact and in mycelium.By scientific research
The modern pharmacology research of mechanism many decades confirms that ganoderma lucidum adjusts blood glucose, control blood pressure, auxiliary for increasing body immunity
Tumor chemoradiotherapy, liver protecting, promote sleep etc. that there is significant curative effect.
Selenium is required a kind of trace mineral element in the mankind and mammal body, is glutathione peroxidase
(GSH-Px), the required composition of the up to 30 various active enzymes such as Selenoprotein P, have anti-oxidant, cardioprotection, treating liver necrosis,
Prevent myopia and cataract, removing toxic substances, improve a variety of psychological applications such as immunity, anti-aging and enhancing reproductive function, according to endless
Full statistics, there is individual countries and regions selenium deficiency more than 40 in the world, and the area that China has 72% belongs to the serious area of selenium deficiency, causes
Pneumonia, liver cancer, stomach cancer etc. turn into compatriots' malignant diseases occurred frequently, and selenium as can effectively antagonism heavy metal, protect liver a kind of function thing
Matter, just paid attention to by more and more consumers.
Selenium element content is not high in the ganoderma lucidum of conventional art production, and ganoderma lucidum yields poorly and biological transformation ratio is low.Using liquid
During fermenting and producing ganoderma lucidum, it is crucial that whether solid culture based formulas used in fermentation is excellent, whether condition of culture fits
Preferably, they directly decide the growth of Ganoderma lucidum mycelium and the formation of metabolite.
The content of the invention
The purpose of the application is a kind of method for providing solid Se-rich lucid ganoderma culture medium and its producing ganoderma lucidum, solves biography
Selenium element content is not high in the ganoderma lucidum of system technology production, and ganoderma lucidum yields poorly and the low problem of biological transformation ratio.
On the one hand, this application provides a kind of solid Se-rich lucid ganoderma culture medium, it is made up of following raw materials：Selenium-rich Juncao, wood
Bits, wheat bran, peanut shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；The weight proportion of the raw material is：Selenium-rich
Juncao 25-35 parts, wood chip 70-80 parts, wheat bran 20-30 parts, peanut shell 10-20 parts, land plaster 2-12 parts, precipitated calcium carbonate 1-11
Part, maltose 10-20 parts, protein peptides 2-12 parts and water 55-65 parts.
Further, the weight proportion of the raw material is：Selenium-rich Juncao 26-34 parts, wood chip 71-79 parts, wheat bran 21-29
Part, peanut shell 11-19 parts, land plaster 3-11 parts, precipitated calcium carbonate 2-10 parts, maltose 11-19 parts, protein peptides 3-11 parts and water
56-64 parts.
Further, the weight proportion of the raw material is：Selenium-rich Juncao 27-33 parts, wood chip 72-78 parts, wheat bran 22-28
Part, peanut shell 12-18 parts, land plaster 4-10 parts, precipitated calcium carbonate 3-9 parts, maltose 12-18 parts, protein peptides 4-10 parts and water
57-63 parts.
Further, the weight proportion of the raw material is：Selenium-rich Juncao 28-32 parts, wood chip 73-77 parts, wheat bran 23-27
Part, peanut shell 13-17 parts, land plaster 5-9 parts, precipitated calcium carbonate 4-8 parts, maltose 13-17 parts, protein peptides 5-9 parts and water 58-
62 parts.
Further, the weight proportion of the raw material is：Selenium-rich Juncao 29-31 parts, wood chip 74-76 parts, wheat bran 24-26
Part, peanut shell 14-16 parts, land plaster 6-8 parts, precipitated calcium carbonate 5-7 parts, maltose 14-16 parts, protein peptides 6-8 parts and water 59-
61 parts.
Further, the weight proportion of the raw material is：30 parts of selenium-rich Juncao, 75 parts of wood chip, 25 parts of wheat bran, peanut shell 15
Part, 7 parts of land plaster, 6 parts of precipitated calcium carbonate, 15 parts of maltose, 7 parts of protein peptides and 60 parts of water.
Further, the selenium-rich Juncao is that the herbaceous plant rich in sodium selenite Compound fertilizer artificial growth crushes and dried
Raw material after dry.
Further, the wood chip is the broad-leaf forest wood chip that purity is more than 90%.
On the other hand, this application provides a kind of method for producing Se-rich lucid ganoderma, comprise the following steps：
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The liquid fermentation and culture temperature is 28 DEG C, rotating speed is 200 revs/min；
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：According to a kind of matching somebody with somebody for above-mentioned solid Se-rich lucid ganoderma culture medium
It is standby than being weighed respectively；Fresh 3.5 parts of soya-bean oil is added, cultivating bag is put into after stirring；By the solid selenium-rich after pack
The steam high temperature that Medium for Ganoderma lucidum is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23-24 DEG C；Humidity is maintained at 40-50%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
From above technical scheme, this application provides a kind of solid Se-rich lucid ganoderma culture medium and its side of production ganoderma lucidum
Method, the solid Se-rich lucid ganoderma culture medium are made up of following raw materials, selenium-rich Juncao, wood chip, wheat bran, peanut shell, land plaster, lightweight
Calcium carbonate, maltose, protein peptides and water；By adding the selenium-rich Juncao containing selenium element in the raw material of culture medium, it is arranged in pairs or groups
Solid Se-rich lucid ganoderma culture medium is made in remaining raw material, then produces Se-rich lucid ganoderma by above-mentioned steps, you can produces rich in selenium element
Ganoderma lucidum, and produce ganoderma lucidum total output it is higher with biological transformation ratio, solve conventional art produce ganoderma lucidum in selenium element contain
Amount is not high, and ganoderma lucidum yields poorly and the low problem of biological transformation ratio.
Brief description of the drawings
In order to illustrate more clearly of the technical scheme of the application, letter will be made to the required accompanying drawing used in embodiment below
Singly introduce, it should be apparent that, for those of ordinary skills, without having to pay creative labor,
Other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is a kind of method flow diagram for producing Se-rich lucid ganoderma that the application provides.
Embodiment
Below in conjunction with the embodiment of the present invention, clear, complete description is carried out to the technical scheme in the embodiment of the present invention,
Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, all peculiar embodiments that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
The purpose of the application is a kind of method for providing solid Se-rich lucid ganoderma culture medium and its producing ganoderma lucidum, solves biography
Selenium element content is not high in the ganoderma lucidum of system technology production, and ganoderma lucidum yields poorly and the low problem of biological transformation ratio.
On the one hand, this application provides a kind of solid Se-rich lucid ganoderma culture medium, it is made up of following raw materials：Selenium-rich Juncao, wood
Bits, wheat bran, peanut shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；The weight proportion of the raw material is：Selenium-rich
Juncao 25-35 parts, wood chip 70-80 parts, wheat bran 20-30 parts, peanut shell 10-20 parts, land plaster 2-12 parts, precipitated calcium carbonate 1-11
Part, maltose 10-20 parts, protein peptides 2-12 parts and water 55-65 parts.
Further, the weight proportion of the raw material is：Selenium-rich Juncao 26-34 parts, wood chip 71-79 parts, wheat bran 21-29
Part, peanut shell 11-19 parts, land plaster 3-11 parts, precipitated calcium carbonate 2-10 parts, maltose 11-19 parts, protein peptides 3-11 parts and water
56-64 parts.
Further, the weight proportion of the raw material is：Selenium-rich Juncao 27-33 parts, wood chip 72-78 parts, wheat bran 22-28
Part, peanut shell 12-18 parts, land plaster 4-10 parts, precipitated calcium carbonate 3-9 parts, maltose 12-18 parts, protein peptides 4-10 parts and water
57-63 parts.
Further, the weight proportion of the raw material is：Selenium-rich Juncao 28-32 parts, wood chip 73-77 parts, wheat bran 23-27
Part, peanut shell 13-17 parts, land plaster 5-9 parts, precipitated calcium carbonate 4-8 parts, maltose 13-17 parts, protein peptides 5-9 parts and water 58-
62 parts.
Further, the weight proportion of the raw material is：Selenium-rich Juncao 29-31 parts, wood chip 74-76 parts, wheat bran 24-26
Part, peanut shell 14-16 parts, land plaster 6-8 parts, precipitated calcium carbonate 5-7 parts, maltose 14-16 parts, protein peptides 6-8 parts and water 59-
61 parts.
Further, the weight proportion of the raw material is：30 parts of selenium-rich Juncao, 75 parts of wood chip, 25 parts of wheat bran, peanut shell 15
Part, 7 parts of land plaster, 6 parts of precipitated calcium carbonate, 15 parts of maltose, 7 parts of protein peptides and 60 parts of water.
Further, the selenium-rich Juncao is that the herbaceous plant rich in sodium selenite Compound fertilizer artificial growth crushes and dried
Raw material after dry.
Further, the wood chip is the broad-leaf forest wood chip that purity is more than 90%.
On the other hand, this application provides a kind of method for producing Se-rich lucid ganoderma, comprise the following steps：
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The liquid fermentation and culture temperature is 28 DEG C, rotating speed is 200 revs/min；
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：According to a kind of matching somebody with somebody for above-mentioned solid Se-rich lucid ganoderma culture medium
It is standby than being weighed respectively；Fresh 3.5 parts of soya-bean oil is added, cultivating bag is put into after stirring；By the solid selenium-rich after pack
The steam high temperature that Medium for Ganoderma lucidum is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23-24 DEG C；Humidity is maintained at 40-50%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment one：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；25 parts of the selenium-rich Juncao, 70 parts of the wood chip, 20 parts of the wheat bran, institute
State 10 parts of peanut shell, 2 parts of the land plaster, 1 part of the precipitated calcium carbonate, 10 parts of the maltose, 2 parts of the protein peptides, institute
State 55 parts of water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；25 parts of the selenium-rich Juncao, 70 parts of the wood chip, the wheat bran
20 parts, 10 parts of the peanut shell, 2 parts of the land plaster, 1 part of the precipitated calcium carbonate, 10 parts of the maltose, the protein peptides
2 parts, 55 parts of the water；And fresh 2 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid Se-rich lucid ganoderma after pack
The steam high temperature that culture medium is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment two：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；26 parts of the selenium-rich Juncao, 71 parts of the wood chip, 21 parts of the wheat bran, institute
State 11 parts of peanut shell, 3 parts of the land plaster, 2 parts of the precipitated calcium carbonate, 11 parts of the maltose, 3 parts of the protein peptides, institute
State 56 parts of water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；26 parts of the selenium-rich Juncao, 71 parts of the wood chip, the wheat bran
21 parts, 11 parts of the peanut shell, 3 parts of the land plaster, 2 parts of the precipitated calcium carbonate, 11 parts of the maltose, the protein peptides
3 parts, 56 parts of the water；And fresh 3 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid Se-rich lucid ganoderma after pack
The steam high temperature that culture medium is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment three：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；27 parts of the selenium-rich Juncao, 72 parts of the wood chip, 22 parts of the wheat bran, institute
State 12 parts of peanut shell, 4 parts of the land plaster, 3 parts of the precipitated calcium carbonate, 12 parts of the maltose, 4 parts of the protein peptides, institute
State 57 parts of water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；27 parts of the selenium-rich Juncao, 72 parts of the wood chip, the wheat bran
22 parts, 12 parts of the peanut shell, 4 parts of the land plaster, 3 parts of the precipitated calcium carbonate, 12 parts of the maltose, the protein peptides
4 parts, 57 parts of the water；And fresh 4 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid Se-rich lucid ganoderma after pack
The steam high temperature that culture medium is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Example IV：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；28 parts of the selenium-rich Juncao, 73 parts of the wood chip, 23 parts of the wheat bran, institute
State 13 parts of peanut shell, 5 parts of the land plaster, 4 parts of the precipitated calcium carbonate, 13 parts of the maltose, 5 parts of the protein peptides, institute
State 58 parts of water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；28 parts of the selenium-rich Juncao, 73 parts of the wood chip, the wheat bran
23 parts, 13 parts of the peanut shell, 5 parts of the land plaster, 4 parts of the precipitated calcium carbonate, 13 parts of the maltose, the protein peptides
5 parts, 58 parts of the water；And fresh 5 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid Se-rich lucid ganoderma after pack
The steam high temperature that culture medium is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment five：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；29 parts of the selenium-rich Juncao, 74 parts of the wood chip, 24 parts of the wheat bran, institute
State 14 parts of peanut shell, 6 parts of the land plaster, 5 parts of the precipitated calcium carbonate, 14 parts of the maltose, 6 parts of the protein peptides, institute
State 59 parts of water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；29 parts of the selenium-rich Juncao, 74 parts of the wood chip, the wheat bran
24 parts, 14 parts of the peanut shell, 6 parts of the land plaster, 5 parts of the precipitated calcium carbonate, 14 parts of the maltose, the protein peptides
6 parts, 59 parts of the water；And fresh 6 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid Se-rich lucid ganoderma after pack
The steam high temperature that culture medium is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment six：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；30 parts of the selenium-rich Juncao, 75 parts of the wood chip, 25 parts of the wheat bran, institute
State 15 parts of peanut shell, 7 parts of the land plaster, 6 parts of the precipitated calcium carbonate, 15 parts of the maltose, 7 parts of the protein peptides, institute
State 60 parts of water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；30 parts of the selenium-rich Juncao, 75 parts of the wood chip, the wheat bran
25 parts, 15 parts of the peanut shell, 7 parts of the land plaster, 6 parts of the precipitated calcium carbonate, 15 parts of the maltose, the protein peptides
7 parts, 60 parts of the water；And fresh 7 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid Se-rich lucid ganoderma after pack
The steam high temperature that culture medium is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment seven：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；31 parts of the selenium-rich Juncao, 76 parts of the wood chip, 26 parts of the wheat bran, institute
State 16 parts of peanut shell, 8 parts of the land plaster, 7 parts of the precipitated calcium carbonate, 16 parts of the maltose, 8 parts of the protein peptides, institute
State 61 parts of water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；31 parts of the selenium-rich Juncao, 76 parts of the wood chip, the wheat bran
26 parts, 16 parts of the peanut shell, 8 parts of the land plaster, 7 parts of the precipitated calcium carbonate, 16 parts of the maltose, the protein peptides
8 parts, 61 parts of the water；And fresh 8 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid Se-rich lucid ganoderma after pack
The steam high temperature that culture medium is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment eight：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；32 parts of the selenium-rich Juncao, 77 parts of the wood chip, 27 parts of the wheat bran, institute
State 17 parts of peanut shell, 9 parts of the land plaster, 8 parts of the precipitated calcium carbonate, 17 parts of the maltose, 9 parts of the protein peptides, institute
State 62 parts of water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；32 parts of the selenium-rich Juncao, 77 parts of the wood chip, the wheat bran
27 parts, 17 parts of the peanut shell, 9 parts of the land plaster, 8 parts of the precipitated calcium carbonate, 17 parts of the maltose, the protein peptides
9 parts, 62 parts of the water；And fresh 9 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid Se-rich lucid ganoderma after pack
The steam high temperature that culture medium is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment nine：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；33 parts of the selenium-rich Juncao, 78 parts of the wood chip, 28 parts of the wheat bran, institute
State 18 parts of peanut shell, 10 parts of the land plaster, 9 parts of the precipitated calcium carbonate, 18 parts of the maltose, 10 parts of the protein peptides,
63 parts of the water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；33 parts of the selenium-rich Juncao, 78 parts of the wood chip, the wheat bran
28 parts, 18 parts of the peanut shell, 10 parts of the land plaster, 9 parts of the precipitated calcium carbonate, 18 parts of the maltose, the albumen
10 parts of peptide, 63 parts of the water；And fresh 10 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid selenium-rich after pack
The steam high temperature that Medium for Ganoderma lucidum is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment ten：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；34 parts of the selenium-rich Juncao, 79 parts of the wood chip, 29 parts of the wheat bran, institute
State 19 parts of peanut shell, 11 parts of the land plaster, 10 parts of the precipitated calcium carbonate, 19 parts of the maltose, 11 parts of the protein peptides,
64 parts of the water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；34 parts of the selenium-rich Juncao, 79 parts of the wood chip, the wheat bran
29 parts, 19 parts of the peanut shell, 11 parts of the land plaster, 10 parts of the precipitated calcium carbonate, 19 parts of the maltose, the albumen
11 parts of peptide, 64 parts of the water；And fresh 11 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid selenium-rich after pack
The steam high temperature that Medium for Ganoderma lucidum is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Embodiment 11：
A kind of solid Se-rich lucid ganoderma culture medium, is made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, peanut shell, gypsum
Powder, precipitated calcium carbonate, maltose, protein peptides and water；35 parts of the selenium-rich Juncao, 80 parts of the wood chip, 30 parts of the wheat bran, institute
State 20 parts of peanut shell, 12 parts of the land plaster, 11 parts of the precipitated calcium carbonate, 20 parts of the maltose, 12 parts of the protein peptides,
65 parts of the water；
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into lucidum liquid under sterile conditions
Body fermentation medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The lucidum liquid
Body fermentation medium is PDA liquid medium；The cultivation temperature of the liquid fermentation is 28 DEG C, rotating speed is 200 revs/min；
The preparation of PDA liquid medium：It is 1L's by volume made of 200 parts of peeled potatoes, 20 parts of glucose and water
Solution, and adjust the pH value of solution to 5.9, then solution is placed in 21 DEG C, is sterilized 30 minutes in 103mpa high-pressure sterilizing pot, both
Obtain PDA liquid medium.
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, egg
White 2 parts of peptide, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：The following raw material, selenium-rich Juncao, wood chip, peanut are weighed respectively
Shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；35 parts of the selenium-rich Juncao, 80 parts of the wood chip, the wheat bran
30 parts, 20 parts of the peanut shell, 12 parts of the land plaster, 11 parts of the precipitated calcium carbonate, 20 parts of the maltose, the albumen
12 parts of peptide, 65 parts of the water；And fresh 12 parts of soya-bean oil is added, put into cultivating bag after stirring；By the solid selenium-rich after pack
The steam high temperature that Medium for Ganoderma lucidum is placed in 100 DEG C is sterilized 7 hours；It can obtain solid Se-rich lucid ganoderma culture medium；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by the spirit of liquid selenium-rich
Sesame strain is uniformly distributed in solid Se-rich lucid ganoderma media surface, after inoculation by cultural hypha, management of producing mushroom, harvesting process,
It can obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature during bacterium germination
Spend for 23 DEG C；Humidity is maintained at 45%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Calculate content, ganoderma lucidum total output and the biological transformation ratio of selenium element in the various embodiments described above production gained ganoderma lucidum.It is raw
Thing conversion ratio, also known as biological efficiency, refer to the weight that the fresh weight of edible mushroom changes material with compost used in the output yield celestial being mushroom (ear)
Measure percentage.Formula is as follows：
Concrete outcome is as follows：
From data in table, from embodiment one to embodiment six, with solid Se-rich lucid ganoderma culture medium, each raw material is matched somebody with somebody
The change of ratio, selenium element content gradually increases in its ganoderma lucidum, and ganoderma lucidum total output also gradually increases with biological transformation ratio；From embodiment
Six to embodiment 11, with the change of raw material proportioning in solid medium, selenium element content, ganoderma lucidum total output and life in ganoderma lucidum
Thing conversion ratio reduces successively.It was therefore concluded that when in solid Se-rich lucid ganoderma culture medium each raw material proportioning it is as follows：Selenium-rich Juncao
30 parts, 75 parts of wood chip, 25 parts of wheat bran, 15 parts of peanut shell, 7 parts of land plaster, 6 parts of precipitated calcium carbonate, 15 parts of maltose, protein peptides 7
60 parts of part and water；The Se-rich lucid ganoderma of the lower production of this proportioning, its selenium element content 184mg/kg, ganoderma lucidum total output 126kg, biology turn
Rate 85.6%, i.e. optimum proportioning.
From above technical scheme：This application provides a kind of solid Se-rich lucid ganoderma culture medium, it is made up of following raw materials：
Selenium-rich Juncao, wood chip, wheat bran, peanut shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；The weight of the raw material
Match and be：It is selenium-rich Juncao 25-35 parts, wood chip 70-80 parts, wheat bran 20-30 parts, peanut shell 10-20 parts, land plaster 2-12 parts, light
Matter calcium carbonate 1-11 parts, maltose 10-20 parts, protein peptides 2-12 parts and water 55-65 parts.Another aspect the application provides a kind of raw
The method for producing Se-rich lucid ganoderma, the lower solid Se-rich lucid ganoderma culture medium prepared of this proportioning, then the ganoderma lucidum produced by the above method, solution
The problem of selenium element content is not high in the ganoderma lucidum of conventional art of having determined production, and ganoderma lucidum yields poorly and biological transformation ratio is low.
Claims (9)
1. a kind of solid Se-rich lucid ganoderma culture medium, it is characterised in that be made up of following raw materials：Selenium-rich Juncao, wood chip, wheat bran, flower
Raw shell, land plaster, precipitated calcium carbonate, maltose, protein peptides and water；The weight proportion of the raw material is：Selenium-rich Juncao 25-35
Part, wood chip 70-80 parts, wheat bran 20-30 parts, peanut shell 10-20 parts, land plaster 2-12 parts, precipitated calcium carbonate 1-11 parts, maltose
10-20 parts, protein peptides 2-12 parts and water 55-65 parts.
A kind of 2. solid Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that the weight proportion of the raw material
For：Selenium-rich Juncao 26-34 parts, wood chip 71-79 parts, wheat bran 21-29 parts, peanut shell 11-19 parts, land plaster 3-11 parts, lightweight carbon
Sour calcium 2-10 parts, maltose 11-19 parts, protein peptides 3-11 parts and water 56-64 parts.
A kind of 3. solid Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that the weight proportion of the raw material
For：Selenium-rich Juncao 27-33 parts, wood chip 72-78 parts, wheat bran 22-28 parts, peanut shell 12-18 parts, land plaster 4-10 parts, lightweight carbon
Sour calcium 3-9 parts, maltose 12-18 parts, protein peptides 4-10 parts and water 57-63 parts.
A kind of 4. solid Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that the weight proportion of the raw material
For：Selenium-rich Juncao 28-32 parts, wood chip 73-77 parts, wheat bran 23-27 parts, peanut shell 13-17 parts, land plaster 5-9 parts, lightweight carbonic acid
Calcium 4-8 parts, maltose 13-17 parts, protein peptides 5-9 parts and water 58-62 parts.
A kind of 5. solid Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that the weight proportion of the raw material
For：Selenium-rich Juncao 29-31 parts, wood chip 74-76 parts, wheat bran 24-26 parts, peanut shell 14-16 parts, land plaster 6-8 parts, lightweight carbonic acid
Calcium 5-7 parts, maltose 14-16 parts, protein peptides 6-8 parts and water 59-61 parts.
A kind of 6. solid Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that the weight proportion of the raw material
For：30 parts of selenium-rich Juncao, 75 parts of wood chip, 25 parts of wheat bran, 15 parts of peanut shell, 7 parts of land plaster, 6 parts of precipitated calcium carbonate, maltose 15
60 parts of part, 7 parts of protein peptides and water.
A kind of 7. solid Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that the selenium-rich Juncao be rich in
The herbaceous plant of sodium selenite Compound fertilizer artificial growth crush and dry after raw material.
8. a kind of solid selenium-rich culturing base according to any one of claim 1 to 7, it is characterised in that the wood chip is pure
Spend the broad-leaf forest wood chip for more than 90%.
A kind of 9. method for producing Se-rich lucid ganoderma, it is characterised in that comprise the following steps：
Step S1：The preparation of liquid Se-rich lucid ganoderma strain：The mycelium of ganoderma lucidum is accessed into ganoderma lucidum liquid hair under sterile conditions
Ferment culture medium, liquid fermentation and culture is carried out, collects all tunnings, i.e., described Liquid Strain of Ganoderma Lucidum；The ganoderma lucidum liquid hair
Ferment culture medium is PDA liquid medium；The liquid fermentation and culture temperature is 28 DEG C, rotating speed is 200 revs/min；
The material quality ratio of the liquid fermentation medium is as follows：200 parts of potato, 50 parts of corn flour, 30 parts of glucose, protein peptides
2 parts, 5 parts of sodium selenite, 70 parts of potassium dihydrogen phosphate, 30 parts of magnesium sulfate；
Step S2：The preparation of solid Se-rich lucid ganoderma culture medium：A kind of solid Se-rich lucid ganoderma training according to claim 1 to 6
The proportioning for supporting base is weighed respectively, standby；Fresh soya-bean oil 2-12 parts are added, cultivating bag is put into after stirring；After packing
Solid Se-rich lucid ganoderma culture medium be placed in 100 DEG C steam high temperature sterilize 7 hours；It can obtain solid Se-rich lucid ganoderma culture
Base；
Step S3：Inoculation：Solid selenium-rich culturing base after step S2 high-temperature sterilizations is cooled to 28 DEG C, by liquid Se-rich lucid ganoderma bacterium
Kind is uniformly distributed in solid Se-rich lucid ganoderma media surface, by cultural hypha, management of producing mushroom, harvesting process after inoculation, you can
Obtain Se-rich lucid ganoderma；
Step S4：Cultural hypha：Say that the solid Se-rich lucid ganoderma culture medium bacterium bag after inoculation moves into culturing room, temperature is during bacterium germination
23-24℃；Humidity is maintained at 40-50%；
Step S5：Management of producing mushroom and harvesting, you can obtain Se-rich lucid ganoderma.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711268568.1A CN107853077A (en) | 2017-12-05 | 2017-12-05 | A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711268568.1A CN107853077A (en) | 2017-12-05 | 2017-12-05 | A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107853077A true CN107853077A (en) | 2018-03-30 |
Family
ID=61704758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711268568.1A Pending CN107853077A (en) | 2017-12-05 | 2017-12-05 | A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107853077A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108307931A (en) * | 2018-03-31 | 2018-07-24 | 广西陆川和合生物科技有限公司 | A kind of Se-rich lucid ganoderma culture material and preparation method thereof |
| CN108586102A (en) * | 2018-07-31 | 2018-09-28 | 合肥仙之峰农业科技有限公司 | A kind of Se-rich lucid ganoderma culture medium and preparation method thereof |
| CN108617400A (en) * | 2018-03-31 | 2018-10-09 | 广西陆川和合生物科技有限公司 | A kind of efficient cultivation method of Se-rich lucid ganoderma |
| CN109076881A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched hericium erinaceus and its application |
| CN109076882A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application |
| CN109769593A (en) * | 2019-03-27 | 2019-05-21 | 儋州牧春绿色生态农业开发有限公司 | A kind of breeding method of Ganoderma lucidum |
| CN113767812A (en) * | 2021-09-23 | 2021-12-10 | 山西农业大学山西功能食品研究院 | Ganoderma strain and culture method of ganoderma lucidum fruiting body |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1129254A (en) * | 1995-02-17 | 1996-08-21 | 周道陆 | Composite granulated substratum for edible (medicinal) mushroom series |
| CN1233482A (en) * | 1998-04-28 | 1999-11-03 | 山东省滨州卫生学校 | Application of selenium-rich glossy ganoderma in medicine for treating psoriasis |
| CN1233389A (en) * | 1998-04-28 | 1999-11-03 | 山东省滨州卫生学校 | Method for cultivating selenium-rich glossy ganoderma |
| CN1316194A (en) * | 2000-04-03 | 2001-10-10 | 仪慧兰 | Method for producing Se-enriched nutritive health-care rice containing ganoderma component |
| CN106242683A (en) * | 2016-08-05 | 2016-12-21 | 井冈山井祥菌草生态科技股份有限公司 | A kind of selenium accumulation of Ganoderma Lucidum culture medium and the method producing Ganoderma thereof |
-
2017
- 2017-12-05 CN CN201711268568.1A patent/CN107853077A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1129254A (en) * | 1995-02-17 | 1996-08-21 | 周道陆 | Composite granulated substratum for edible (medicinal) mushroom series |
| CN1233482A (en) * | 1998-04-28 | 1999-11-03 | 山东省滨州卫生学校 | Application of selenium-rich glossy ganoderma in medicine for treating psoriasis |
| CN1233389A (en) * | 1998-04-28 | 1999-11-03 | 山东省滨州卫生学校 | Method for cultivating selenium-rich glossy ganoderma |
| CN1316194A (en) * | 2000-04-03 | 2001-10-10 | 仪慧兰 | Method for producing Se-enriched nutritive health-care rice containing ganoderma component |
| CN106242683A (en) * | 2016-08-05 | 2016-12-21 | 井冈山井祥菌草生态科技股份有限公司 | A kind of selenium accumulation of Ganoderma Lucidum culture medium and the method producing Ganoderma thereof |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108307931A (en) * | 2018-03-31 | 2018-07-24 | 广西陆川和合生物科技有限公司 | A kind of Se-rich lucid ganoderma culture material and preparation method thereof |
| CN108617400A (en) * | 2018-03-31 | 2018-10-09 | 广西陆川和合生物科技有限公司 | A kind of efficient cultivation method of Se-rich lucid ganoderma |
| CN109076881A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched hericium erinaceus and its application |
| CN109076882A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application |
| CN109076882B (en) * | 2018-06-29 | 2021-09-03 | 江西天和食用菌开发有限公司 | Culture method and application of selenium-rich edible fungus mycelia |
| CN108586102A (en) * | 2018-07-31 | 2018-09-28 | 合肥仙之峰农业科技有限公司 | A kind of Se-rich lucid ganoderma culture medium and preparation method thereof |
| CN109769593A (en) * | 2019-03-27 | 2019-05-21 | 儋州牧春绿色生态农业开发有限公司 | A kind of breeding method of Ganoderma lucidum |
| CN113767812A (en) * | 2021-09-23 | 2021-12-10 | 山西农业大学山西功能食品研究院 | Ganoderma strain and culture method of ganoderma lucidum fruiting body |
| CN113767812B (en) * | 2021-09-23 | 2023-04-25 | 山西农业大学山西功能食品研究院 | Ganoderma lucidum strain and method for culturing red-meat ganoderma lucidum fruiting body |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107853077A (en) | A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum | |
| CN103304333B (en) | Culture medium for lucid ganoderma and culture method | |
| CN102612985A (en) | Production technology for cordyceps militaris mycelium | |
| CN105248150B (en) | A kind of method of cordycepin content in raising fruiting bodies of cordyceps militaris | |
| CN102301912B (en) | Method for culturing Cordyceps militaris by using highland barley as main raw material | |
| CN105543104A (en) | Optimization method of solid culture medium for artificial acclimation and cultivation of wild Isaria cicadae Miquel | |
| CN105565934A (en) | Ganoderma lucidum culture medium | |
| CN101991045A (en) | Cordyceps militaris rice and solid fermentation preparation method thereof | |
| CN101897270A (en) | Production technology of Cordyceps sinensis mycelium | |
| CN104478546A (en) | Method for utilizing vitex negundo var ineica scrap to culture hericium erinaceus | |
| CN109997608A (en) | A kind of implantation methods of edible mushroom | |
| CN104541967B (en) | A kind of production method of the shiitake mushroom hypha for extracting lentinan | |
| CN102870594B (en) | Method for culturing hericium erinaceus through tobacco stem facility | |
| CN102812847A (en) | Cultivating method of pleurotus nebrodensis | |
| CN104885787A (en) | Planting method for cordyceps militaris sporocarp high in active matter content | |
| CN102318544A (en) | A kind of Cordyceps militaris ciltivating process | |
| CN106631344A (en) | Lucid ganoderma bag cultivation culture medium capable of increasing content of ganoderan and selenium | |
| CN104429621B (en) | A kind of production method of the hericium mycelium for extracting Hericium Erinaceus Polysaccharide | |
| CN109134081A (en) | A kind of bio-feritlizer and preparation method thereof rich in functional polysaccharide | |
| CN103005437B (en) | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation | |
| CN100513549C (en) | Liquid mushroom producing method and fermenting set | |
| CN104177182A (en) | Cultural method for producing cordyceps militaris fruiting body by use of corn cobs | |
| CN103283480B (en) | Method for growing cordyceps sinensis fruiting body directly in liquid medium | |
| CN103371044A (en) | Selenium-enriched cordyceps flower production method | |
| CN108676733A (en) | The mycelial cultural method of phellinus linteus and its application on health products |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180330 |
|
| RJ01 | Rejection of invention patent application after publication |