CN101897270A

CN101897270A - Production technology of Cordyceps sinensis mycelium

Info

Publication number: CN101897270A
Application number: CN200910141359XA
Authority: CN
Inventors: 何寒; 吴烈秋
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-05-25
Filing date: 2009-05-25
Publication date: 2010-12-01

Abstract

The invention discloses a fermentation culture method of Cordyceps sinensis mycelium, which mainly comprises the procedures of preparation of liquid culture medium, culture of a large number of cocci (hypha) via a rotary shaking table, and acquisition of more mycelia with better quality by means of secondary fermentation of crop leftovers and liquid spawns. The production technology according to the invention solves the defects of high fermentation conditions, complex raw materials used for culture medium, high cost and the like in the prior art, and implements, via the rotary shaking table, the culture, which is simple in conditions, easy in obtaining raw materials, short in period, high in efficiency and stable. The mycelium produced has excellent quality, powder of the Cordyceps sinensis mycelium produced according to the method is consistent, in major components and pharmacological properties, with natural Cordyceps sinensis, and the technology can be applied to large-scale production, is low in production cost and includes high development value.

Description

A kind of production technology of Cordyceps sinensis mycelium

Technical field

The invention belongs to the biofermentation field, specifically a kind of production technology of cordyceps mycelia.

Background technology

Cordyceps sinensis claims CORDYCEPS again, Chinese caterpillar fungus, it is that section ergot fungus cordyceps sinensis Cordycrpssinensis (Berk.) Sacc. colonizes in the stroma on the larvae section insect larvae and the complex of larva corpse, be a kind of traditional famous and precious tonic Chinese herbal medicine material, be listed as three big invigorants with natural ginseng, pilose antler.Its property of medicine gentleness, equal edible throughout the year, the old or young, sick, weak, virtual person is all suitable, than the invigorant of other kinds medical value is widely arranged, since ancient times, people just it as invigorant and pharmaceutical products,

Cordyceps sinensis mainly is grown in high height above sea level, high and cold area, and the formation of growing environment is very harsh and complicated, and people constantly increase and irrational excavating the demand of Chinese caterpillar fungus in addition, cause the Chinese caterpillar fungus natural resources to gradually reduce.Therefore, in order to solve the medicine source, people have to by manually cultivating Chinese caterpillar fungus, but for these years, artificial cultivation Chinese caterpillar fungus complex manufacturing, with short production cycle, yield poorly and perplexing people always.

By the effort of researcher, a kind of brand-new worm grass product---cordyceps mycelia has developed, and this mycelium just is to use deep fermentation to obtain.

Agricultural University Of Anhui had once carried out analysis of effective component to cordyceps mycelia, its result shows: the amino acid kind is consistent with the Cordyceps sinensis crude drug in the mycelium, but total amino acid content and TEAA are apparently higher than the Cordyceps sinensis crude drug, the ratio that essential amino acid accounts for total amino acid also is higher than the latter, the content of threonine, valine, isoleucine, leucine, lysine is farther far above the latter in the essential amino acid, and the content of crude protein, total reducing sugar and mannitol all is higher than the Cordyceps sinensis crude drug in the mycelium; During mycelial fatty acid is formed, unsaturated fatty acid accounts for 85.38% of fatty acid composition, wherein polyvalent unsaturated fatty acid such as linoleic acid, linolenic acid accounts for 58.87% of fatty acid composition, and linoleic content accounts for (51.04%) over half that whole fatty acid is formed; Fermentation mycelium contains abundant to the very important Zn of health, metallic elements such as Fe, Ca, and the content with Ca is height especially.

Use the deep fermentation cordyceps mycelia, characteristics such as the cycle is short, efficient is high, stable are the main developing direction of artificial culture worm grass product.

In the existing technology, number of patent application is that 02134573.2 Deel-layer fermentation process for culturing Brazilian cordyceps mycelia and number of patent application are 93104709.9 producing process of Chinese caterpillar fungus hypha fermentation, all be to adopt three grades of submerged fermentations, finish whole fermentation process and need 12-15 days, production cycle is long, use expensive fermentation tank to cultivate, fermentation condition height, medium use raw material complexity, cost height.What number of patent application was that 01141350.6 a kind of CORDYCEPS bacterium powder class green feed additive adopts is solid fermentation, and production technology is simple, but cultural hypha is needed general 2 months time well, and the production cycle is longer.

Summary of the invention

The object of the present invention is to provide a kind of fermentation tank that need not involve great expense, and be to use rotary shaking table to cultivate, this pattern condition of culture is simple, raw material is easy to get, the cycle is short, efficient is high, stable etc.The mycelium product of producing is of fine quality and the generation cost is very low.

The present invention is achieved through the following technical solutions:

1, screening meets suitability for industrialized production, mycelial growth is vigorous and FFI slant strains.

2, obtaining liq medium: 6 jin of potatos, 3 jin of corn flour, 1 jin of analysis for soybean powder, 2 jin of white sugar, ascorbic acid 60 grams, urea 80 grams, 100 jin in water.Earlier potato is broken into slurry, in water, put into corn flour, analysis for soybean powder, white sugar, ascorbic acid, urea successively then, poach is opened standby with slow fire.

3, system is prepared, need not filtered liquid medium branch and install in the Clear glass bottles and jars of high temperature high voltage resistant, charge weight is for holding half of tool actual capacity, then with cotton with the bottleneck jam-pack.Next sterilization, sterilization time is: autoclave sterilization 40-50 minute, normal-pressure sterilization 4-5 hour.

4, under gnotobasis, insert bacterial classification, and static cultivation 3-5 days.

5, bacterial classification sprout, free of contamination liquid nutrient medium moves on to rotary shaking table and dynamically cultivates, the shaking table rotary speed is that 150-300 changes, the rotation of shaking table is also rotated liquid nutrient medium thereupon, the clinker collision of the rotation of bacterial classification liquid medium within and medium the inside constantly point is torn into several bacterium balls (sheet, silk) down, and acquisition has the liquid spawn of enriching bacterium ball (sheet, silk) after 3 days.

6, carry out secondary fermentation and cultivate, medium is: 50 jin in rice bran, 50 jin in wheat bran, 4 jin of sugar, 120 jin of cultured liquid spawns.Above-mentioned various mixing of materials are stirred, and the humidity regulation of mixture is arrived 55-60%.

7, mixture is layered on the floor, its THICKNESS CONTROL hides with sterile woven bag then and carries out aerobic fermentation at the 15-20 centimetre.

8, both finished whole fermentation process after 2-3 days when charge level covers with pure white mycelia, oven dry promptly obtains the cordyceps mycelia of high-quality under 60 degree.

Compared with prior art, the existing following advantage of the present invention:

1, the raw material that uses of the present invention simply, easily purchase, cost is low;

2, the appearance tool that uses of the present invention also simply, easily purchase, cost is low;

3, the present invention adopts rotary shaking table to carry out the submerged fermentation cultivation, and liquid spawn does not need multistage cultivation in incubation, not only shortens the production cycle, has reduced the risk because of the easy infection of multistage switching spawn culture simultaneously yet;

4, the present invention cultivates by secondary fermentation, use be extensively and inexpensive agricultural byproduct, after cultivating by secondary fermentation, not only make mycelium become more more vigorous, inexpensive agricultural byproduct is rised in value;

5, strain cultivation process of the present invention only must 3-5 days, and carrying out secondary fermentation more also need only 2-3 day, and the whole production cycle is also with regard to 5-8 days.

Embodiment

Below in conjunction with embodiment method of the present invention is further specified.

2, obtaining liq medium: 6 jin of potatos, 3 jin of corn flour, 1 jin of analysis for soybean powder, 2 jin of white sugar, ascorbic acid 60 grams, urea 80 grams, 100 jin in water.Earlier potato is broken into slurry, admix then in the water, put into corn flour, analysis for soybean powder, white sugar, ascorbic acid, urea successively simultaneously, poach is opened with slow fire.

Need not when 3, medium uses to filter, but need before using the liquid nutrient medium that system prepares is mixed thoroughly, slag liquid wherein is evenly distributed, divide then and install in high temperature high voltage resistant and the transparent 20 liters of glass containers, the amount of liquid nutrient medium of packing into is 10 liters, uses cotton jam-pack bottleneck after bottleneck is wiped clean again.Next begin sterilization, sterilization time is: carried out normal-pressure sterilization 4-5 hour under the pressure of 1.5 squares/cm autoclave sterilization 40-50 minute.

4, under gnotobasis, insert bacterial classification, the bacterial classification of access want fritter and thin, when inserting action light and slow, the bacterial classification of access is sunk to inside the liquid.The bacterial classification that inserts should be wherein at a place, but is evenly distributed on the face of liquid nutrient medium.Take static cultivation 2 days after bacterial classification connects, on the one hand bacterial classification is recovered and sprouts, whether have or not infection after can observing the access bacterial classification on the other hand.

5, bacterial classification sprout, free of contamination liquid nutrient medium moves on to rotary shaking table and dynamically cultivates, the shaking table rotary speed is that 150-300 changes, under the drive of shaking table, liquid nutrient medium also rotates thereupon, and producing a large amount of oxygen, the clinker collision of the rotation of bacterial classification liquid medium within and medium the inside constantly point is torn into several bacterium balls (sheet, silk) down.Bacterial classification is being grown rapidly in the nutritious liquid nutrient medium and under the polyoxy environment, obtains to have the liquid spawn of enriching bacterium ball (sheet, silk) after 3 days.

6, strain cultivation just can be carried out the secondary fermentation cultivation in the back well, and medium is: 50 jin in rice bran, 50 jin in wheat bran, 4 jin of sugar, 120 jin of cultured liquid spawns.Above-mentioned various materials in clean, sterile floor mixing and stirring, and the humidity regulation of mixture to 55-60%.

7, mixture is layered on clean, the sterile floor, its THICKNESS CONTROL is in the 15-20 centimetre, and accomplishes: the shop is thicker when day cool, and the shop approaches when day hot.Hide with sterile woven bag then and carry out aerobic fermentation.

8, when charge level covers with pure white mycelia, both finished fermentation process after 2-3 days, the oven dry under 60 degree of cultured mycelium had promptly been obtained the cordyceps mycelia of super quality and competitive price.

Claims

1. production technology of Cordyceps sinensis mycelium is characterized in that may further comprise the steps:

(1) screening meets suitability for industrialized production, mycelial growth is vigorous and FFI slant strains.

(2) obtaining liq medium: 6 jin of potatos, 3 jin of corn flour, 1 jin of analysis for soybean powder, 2 jin of white sugar, ascorbic acid 60 grams, urea 80 grams, 100 jin in water.Earlier potato is broken into slurry, in water, put into corn flour, analysis for soybean powder, white sugar, ascorbic acid, urea successively then, poach is opened standby with slow fire.

(3) system is prepared, need not filtered liquid medium branch and install in the Clear glass bottles and jars of high temperature high voltage resistant, charge weight is for holding half of tool actual capacity, then with cotton with the bottleneck jam-pack.Next sterilization, sterilization time is: autoclave sterilization 40-50 minute, normal-pressure sterilization 4-5 hour.

(4) under gnotobasis, insert bacterial classification, and static cultivation 3-5 days.

(5) bacterial classification sprout, free of contamination liquid nutrient medium moves on to rotary shaking table and dynamically cultivates, the shaking table rotary speed is that 150-300 changes, the rotation of shaking table is also rotated liquid nutrient medium thereupon, the clinker collision of the rotation of bacterial classification liquid medium within and medium the inside constantly point is torn into several bacterium balls (sheet, silk) down, and acquisition has the liquid spawn of enriching bacterium ball (sheet, silk) after 3 days.

(6) carry out secondary fermentation and cultivate, medium is: 50 jin in rice bran, 50 jin in wheat bran, 4 jin of sugar, 120 jin of cultured liquid spawns.Above-mentioned various mixing of materials are stirred, and the humidity regulation of mixture is arrived 55-60%.

(7) mixture is layered on the floor, its THICKNESS CONTROL hides with sterile woven bag then and carries out aerobic fermentation at the 15-20 centimetre.

(8) both finished whole fermentation process after 2-3 days when charge level covers with pure white mycelia, oven dry promptly obtains the cordyceps mycelia of high-quality under 60 degree.