CN102835663B - Chinese caterpillar fungus mycelium powder production process - Google Patents
Chinese caterpillar fungus mycelium powder production process Download PDFInfo
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Abstract
The invention provides a Chinese caterpillar fungus mycelium powder production process. The process comprises the following steps of: (I) preparing a grain medium; (II) preparing liquid strains; (III) inoculating the grain medium; (IV) sending the bags inoculated with the strains to a fungi growing room; and (V) pulverizing dried chickpea mycelia, wheat mycelia and black buckwheat rice mycelia with an ultramicro pulverizer based on 50% of wheat, 30% of chickpea and 20% of black buckwheat rice until the fineness is above 150 meshes and packaging the powder, thus obtaining the finished product. The production process has the beneficial effects that chickpea, wheat and black buckwheat silkworm chrysalis powder are adopted as the media and liquid is adopted for inoculation, bagging and production; the production process has the characteristics of short production cycle, low production cost, good taste, easiness in digestion and low rice; and the produced Chinese caterpillar fungus mycelium powder has the functions of immunoregulation and lung protection and kidney tonifying.
Description
Technical field
The invention belongs to health product technology field, relate in particular to the new technology that a kind of mycelium powder of sinensis is produced.
Background technology
Cordyceps Militaris is under the jurisdiction of mycota, Eumycota, and from profile, the part picture grass that Chinese caterpillar fungus bassets, be embedded in the corpse that underground part is bat moth insect larvae, and " grass " infested head is upper, and " worm " is like careless root.Therefore, the biologist is according to the principle of classification of living species and the morphological character of itself, it is defined as to section ergot fungus cordyceps sinensis and colonizes in stroma on the Hepialidae insect larvae and the complex of larva corpse.It mainly is distributed on the plateau and mountains of China's height above sea level more than 4000 meters, has accumulated snow, draining Alpine meadow area well, and its needed growth conditions is extremely harsh.Chinese caterpillar fungus output is being successively decreased every year, and pharmacology and clinical efficacy take up an area position in the modern medicine health centre more and more higher.Great approximately 0.5 gram of Chinese caterpillar fungus is sold for more than 100 yuan on market, and the normal person keeps healthy requirement more than 1 gram, due to the height of price, is difficult to reach the effect of Healthcare.The production and processing technology of Chinese caterpillar fungus mainly contains two kinds now, and the one,, separate and cultivate bacterial classification on cordyceps militaris sporocarp, be connected on bacterial classification on silkworm chrysalis or grow fructification on rice, the about 80-120 days of growth time, and need be very large with space, output is very little, and price comparison is high.While gathering, major part is abandoned with silkworm chrysalis and the rice of culture medium.The 2nd,, to Cordyceps sinensis polysaccharide in cordyceps mycelia, the extraction of cordycepic acid active ingredient, most enterprises is taked now, dried silkworm chrysalis meal, potato, potassium dihydrogen phosphate, brown sugar is as culture medium, and liquid spawn is cultivated, large-scale retort production, centrifuge separates, and finally extracts concentrated method, make a lot of trace elements useful to human body along with fluid loss, the value of Chinese caterpillar fungus does not maximize the use, and the valuable product per kilogram of producing is more than 20,000 yuan, the mouthfeel extreme difference, can only encapsulatedly drink.
Summary of the invention
The present invention provides a kind of mycelium powder of sinensis production technology for addressing the above problem.
Technical scheme of the present invention is achieved in that
A kind of mycelium powder of sinensis production technology comprises the following steps:
(1), make grain culture medium:
(1), select slave special (being commonly called as peach beans, chick-pea), wheat, black bitter buckwheat, dried silkworm chrysalis meal, at first slave spy, wheat, black bitter buckwheat are cleaned up, put into respectively the container steam heated, temperature is controlled at 100 ℃-105 ℃, pressure 0.03-0.05 MPa, the special 60-65 of digestion time slave minute, wheat 20-25 minute, black bitter buckwheat 8-10 minute;
(2), put fresh silkworm chrysalis into baking oven, temperature is controlled at 90 ℃-100 ℃, dries 8-10 hour, until water content lower than 2%, temperature is while being down to 20 ℃-25 ℃, with 60 purpose pulverizers pulverizing;
(3), after boiling, slave spy, wheat, black bitter buckwheat stir with dried silkworm chrysalis meal respectively, its ratio dried silkworm chrysalis meal respectively accounts for 5-7%, use automatic packer to be respectively charged in bag, every bag of 1.5-1.6 kilogram, then put sterilizing in sterilization tank into, temperature is controlled at 120 ℃-122 ℃, pressure 0.13-0.15 MPa, time 130-150 minute;
(4), when the sterilizing Pressure Drop to zero the time, open the sterilization tank door, send into the cooling chamber cooling, send into the aseptic inoculation chamber when temperature is down to 18 ℃-20 ℃, wait for inoculation, the inoculation indoor temperature is 19-21 ℃, positive pressure operation;
(2), make liquid spawn:
(1) worm summer in, seed selection high-quality winter entomogenous fungi kind is female plants;
(2), prepare mother culture media: mother culture media is prepared burden by following weight ratio: dried silkworm chrysalis meal 1.4-1.5%, potato 28-29%, wheat bran 56-57%, brown sugar 5-6%, potassium dihydrogen phosphate 0.5-0.6%, magnesium sulfate 0.2-0.3%, agar 5-6%, VB
11-2%, VB
60.5-0.6%, peeling potatoes stripping and slicing (5mm*5mm) is put into to water together with dried silkworm chrysalis meal, wheat bran and boil, after slow fire 20-25 minute, by 4 layers of filtered through gauze, supply water to 1000 milliliter after filtration, in order by brown sugar, potassium dihydrogen phosphate, magnesium sulfate, agar, VB
1, VB
6add respectively, the culture medium prepared is distributed into while hot and connects the bacterium box, loading amount height 0.3mm-0.5mm, the diameter 8cm of box, during sterilizing, lid got well and use each wrapping of brown paper tightly to put into the high-pressure sterilizing pot sterilizing, keep flat, temperature is controlled at 120 ℃-122 ℃, pressure 0.13-0.15 MPa, sterilizing 35-40 minute, during pot pressure back to zero subject to sterilization, taking-up connects the bacterium box, temperature descends, after connecing the interior culture medium solidifying of bacterium box, whether thorough in order to check sterilizing, to connect the bacterium box and be placed under the environment of 32 ℃-35 ℃, cultivate 45-48 hour, the culture medium connect in the bacterium box can be used without the miscellaneous bacteria bacterium colony,
(3), by the qualified bacterium box that connects, put into superclean bench, open ultraviolet lamp and irradiate 30-35 minute, alcohol with 75% is to work top, hand, inoculating tools etc. carry out disinfection, with alcolhol burner to transfer needle, the vaccinating lancet sterilization, then the female water of planting of Chinese caterpillar fungus is cleaned to its surperficial silt, then with cotton ball soaked in alcohol, repeatedly clear up its surface, scrape off the skin on Chinese caterpillar fungus surface with the vaccinating lancet after sterilization, in superclean bench, it is cut to one section by 1 millimeters long, each connects the bacterium box and puts four segments, two sections of polypides, two sections of cursive script, by 3 centimeters of square pitch, put, then put into insulating box, 20 ℃-22 ℃ of temperature, cultivate 5-7 days, when the little segment length of the polypide connect on culture medium in inoculating box has the white mycelia of one centimeter size of diameter, again it is moved and receives in slant tube, the culture medium of test tube is the same with the culture medium way that connects the bacterium box, while hot the test tube after sterilizing is put into to inclined-plane, put into insulating box after solidifying, temperature is controlled at 20 ℃-22 ℃, cultivate 5-7 days, treat that the inclined-plane mycelia covers with, plant when producing in a large number and use as mother,
(4), make the shaking flask culture medium: the shaking flask culture medium is prepared burden according to following weight ratio: dried silkworm chrysalis meal 1-2%, potato 25-35%, wheat bran 55-65%, brown sugar 5-7%, potassium dihydrogen phosphate 0.5-0.7%, magnesium sulfate 0.2-0.4%, VB
11-2 %, VB
60.3-0.5%, sterilizing is identical with the Mother culture based method, when temperature drops to 19 ℃-22 ℃, send in the clean work station after sterilization mother is planted from test tube and takes out 4 bacterium pieces that area is 5mm*5mm, be placed on the shaking flask liquid level, send 20 ℃ of insulating boxs to, cultivate 72-96 hour, be placed on shaking table until the bacterium piece when surrounding grows into circle (15mm) and shake, 100-120 per minute turns, rotating and culturing 65-72 hour, having mycelium pellet evenly to be formed on can be stand-by in liquid;
(5), make the retort culture medium: the retort culture medium is prepared burden by following weight ratio: soya bean 27-28%, starch 54-55%, wheat bran 13-14%, potassium dihydrogen phosphate 0.2-0.3%, magnesium sulfate 0.1-0.2%, peptone 1-2%, soya-bean oil 2-3%, the soya bean making beating is filtered, starch is with after cold water dilution (ratio 1:20), being heated to 60 ℃-65 ℃, adding water with soya-bean milk mixing wheat bran boils and boils 15-20 minute, after filtering and soya-bean milk starch mix and blend be sequentially added into again potassium dihydrogen phosphate, magnesium sulfate, peptone, after precipitation, do not pour retort into, moisturizing to 200 liter, while heating to 80 ℃-85 ℃, open intake valve and blow 5-8 minute, the air foam of generation and impurity are removed, add again soya-bean oil, shut the reaction cover, continue to heat up, when temperature rises to 100 ℃-105 ℃, exhaust once, when temperature reaches 120 ℃-122 ℃, pressure 0.13-0.15 MPa, the heat produced in tank, importing and exporting pipeline from respirator passes through, sterilizing 30-35 minute, close the respirator terminal valve, keep 120 ℃-122 ℃ of temperature, pressure 0.13-0.15 MPa, 40-45 minute, start cooling with cooling water, while making temperature be down to 18 ℃-20 ℃, prepare inoculation,
(6), meet bacterial classification mouth place on the top of retort and light the alcohol burning things which may cause a fire disaster, docking shaking flask bacterial classification inoculation pipeline in fire, go out after the connecting source of reducing internal heat is raised shaking flask and is allowed bacterial classification flow into retort, off-response tank inoculation valve, enter normal operating, pressure 0.05-0.07 MPa, 19 ℃-22 ℃ of temperature, cultivate 90-96 hour, the sample examination bacterial classification reaches requirement, prepares to inoculate to grain culture medium;
(3), grain culture medium inoculation: the bacterial classification output channel of retort is with after inoculation device is connected, and through high temperature of 120 DEG C-122, ℃ gas carries out sterilizing 30-35 minute to it, and temperature is fallen only 18 ℃-22 ℃, starts to inoculate every bag of inoculum concentration 15-20 milliliter;
(4) bag that, connects bacterial classification is sent into long bacterium chamber, 19 ℃-22 ℃ of temperature, submerged fermentation 15 days, turn over layer on the 5th day and check, within the 10th day, the long Bai Shiyong hand of bacterial classification is to every bag of careful pinching, destroy its normal mycelial growth, thereby produce more bacterium head, and make the more vigorous of mycelia length, in the time of 15 days, the bacterium bag has been pinched motionless with hand, when the surface mycelia has purpling red, broken bag is dried;
(5), the special mycelia of slave by drying, wheat mycelia, black bitter buckwheat mycelia be according to wheat 40-50%, the special 30-40% of slave, black bitter buckwheat 10-20% ratio are pulverized with micronizer, more than fineness reaches 150 orders, packing is finished product;
A kind of mycelium powder of sinensis production technology comprises the following steps:
(1), make grain culture medium:
(1), select slave special (being commonly called as peach beans, chick-pea), wheat, black bitter buckwheat, dried silkworm chrysalis meal, at first slave spy, wheat, black bitter buckwheat are cleaned up, put into respectively the container steam heated, temperature is controlled at 103 ℃, pressure 0.03 MPa, special 60 minutes of digestion time slave, wheat 20 minutes, black bitter buckwheat 8 minutes;
(2), put fresh silkworm chrysalis into baking oven, temperature is controlled at 95 ℃, dry 8 hours, until water content lower than 2%, temperature is while being down to 23 ℃, with 60 purpose pulverizers, pulverize;
(3), slave spy, wheat, black bitter buckwheat stir with dried silkworm chrysalis meal respectively after boiling, its ratio dried silkworm chrysalis meal respectively accounts for 6%, uses automatic packer to be respectively charged in bag, 1.55 kilograms every bag, then put sterilizing in sterilization tank into, temperature is controlled at 121 ℃, pressure 0.15 MPa, 150 minutes time;
(4), when the sterilizing Pressure Drop to zero the time, open the sterilization tank door, send into the cooling chamber cooling, send into the aseptic inoculation chamber when temperature is down to 20 ℃, wait for inoculation, the inoculation indoor temperature is 20 ℃, positive pressure operation;
(2), make liquid spawn:
(1) worm summer in, seed selection high-quality winter entomogenous fungi kind is female plants;
(2), prepare mother culture media: mother culture media is prepared burden by following weight ratio: dried silkworm chrysalis meal 1.42%, potato 28.41%, wheat bran 56.82%, brown sugar 5.68%, potassium dihydrogen phosphate 0.57%, magnesium sulfate 0.28%, agar 5.12%, VB
11.14%, VB
60.57%, peeling potatoes stripping and slicing (5mm*5mm) to be put into to water together with dried silkworm chrysalis meal, wheat bran and boil, slow fire, after 20 minutes, by 4 layers of filtered through gauze, is supplied water to 1000 milliliter, in order by brown sugar, potassium dihydrogen phosphate, magnesium sulfate, agar, VB after filtration
1, VB
6add respectively, the culture medium prepared is distributed into while hot and connects the bacterium box, loading amount height 0.4mm, the diameter 8cm of box, during sterilizing, lid got well and use each wrapping of brown paper tightly to put into the high-pressure sterilizing pot sterilizing, keeping flat, temperature is controlled at 121 ℃, pressure 0.15 MPa, sterilizing 40 minutes, during pot pressure back to zero subject to sterilization, take out and connect the bacterium box, temperature descends, connect in the bacterium box after culture medium solidifying, whether thorough in order to check sterilizing, will connect the bacterium box and be placed under the environment of 35 ℃, cultivate 48 hours, the culture medium connect in the bacterium box can be used without the miscellaneous bacteria bacterium colony;
(3), by the qualified bacterium box that connects, put into superclean bench, opening ultraviolet lamp irradiates 30 minutes, alcohol with 75% is to work top, hand, inoculating tools etc. carry out disinfection, with alcolhol burner to transfer needle, the vaccinating lancet sterilization, then the female water of planting of Chinese caterpillar fungus is cleaned to its surperficial silt, then with cotton ball soaked in alcohol, repeatedly clear up its surface, scrape off the skin on Chinese caterpillar fungus surface with the vaccinating lancet after sterilization, in superclean bench, it is cut to one section by 1 millimeters long, each connects the bacterium box and puts four segments, two sections of polypides, two sections of cursive script, by 3 centimeters of square pitch, put, then put into insulating box, 21 ℃ of temperature, cultivate 6 days, when the little segment length of the polypide connect on culture medium in inoculating box has the white mycelia of one centimeter size of diameter, again it is moved and receives in slant tube, the culture medium of test tube is the same with the culture medium way that connects the bacterium box, while hot the test tube after sterilizing is put into to inclined-plane, put into insulating box after solidifying, temperature is controlled at 21 ℃, cultivate 6 days, treat that the inclined-plane mycelia covers with, plant when producing in a large number and use as mother,
(4), make the shaking flask culture medium: the shaking flask culture medium is prepared burden according to following weight ratio: dried silkworm chrysalis meal 1.5%, potato 30%, wheat bran 60%, brown sugar 6%, potassium dihydrogen phosphate 0.6%, magnesium sulfate 0.3%, VB
11.2 %, VB
60.4 %, sterilizing is identical with the Mother culture based method, when temperature drops to 21 ℃, send in the clean work station after sterilization mother is planted from test tube and takes out 4 bacterium pieces that area is 5mm*5mm, be placed on the shaking flask liquid level, send 20 ℃ of insulating boxs to, cultivate 88 hours, be placed on shaking table until the bacterium piece when surrounding grows into circle (15mm) and shake, per minute 110 turn, rotating and culturing 72 hours, having mycelium pellet evenly to be formed on can be stand-by in liquid;
(5), make the retort culture medium: the retort culture medium is prepared burden by following weight ratio: soya bean 27.3%, starch 54.6%, wheat bran 13.6%, potassium dihydrogen phosphate 0.27%, magnesium sulfate 0.14%, peptone 1.4%, soya-bean oil 2.7%, the soya bean making beating is filtered, starch is with after cold water dilution (ratio 1:20), being heated to 63 ℃, adding water with soya-bean milk mixing wheat bran boils and boils 15 minutes, after filtering and soya-bean milk starch mix and blend be sequentially added into again potassium dihydrogen phosphate, magnesium sulfate, peptone, after precipitation, do not pour retort into, moisturizing to 200 liter, while heating to 80 ℃, opening intake valve blows 5 minutes, the air foam of generation and impurity are removed, add again soya-bean oil, shut the reaction cover, continue to heat up, when temperature rises to 103 ℃, exhaust once, when temperature reaches 121 ℃, pressure 0.15 MPa, the heat produced in tank, importing and exporting pipeline from respirator passes through, sterilizing 30 minutes, close the respirator terminal valve, keep 121 ℃ of temperature, pressure 0.15 MPa, 40 minutes, start cooling with cooling water, while making temperature be down to 20 ℃, prepare inoculation,
(6), meet bacterial classification mouth place on the top of retort and light the alcohol burning things which may cause a fire disaster, docking shaking flask bacterial classification inoculation pipeline in fire, go out after the connecting source of reducing internal heat is raised shaking flask and is allowed bacterial classification flow into retort, off-response tank inoculation valve, enter normal operating, pressure 0.06 MPa, 21 ℃ of temperature, cultivate 96 hours, the sample examination bacterial classification reaches requirement, prepares to inoculate to grain culture medium;
(3), grain culture medium inoculation: the bacterial classification output channel of retort, with after inoculation device is connected, carries out sterilizing 30 minutes through high temperature of 120 DEG C gas to it, and temperature is down to 22 ℃, starts to inoculate 15 milliliters of every bag of inoculum concentrations;
(4) bag that, connects bacterial classification is sent into long bacterium chamber, 21 ℃ of temperature, submerged fermentation 15 days, turn over layer on the 5th day and check, within the 10th day, the long Bai Shiyong hand of bacterial classification is to every bag of careful pinching, destroy its normal mycelial growth, thereby produce more bacterium head, and make the more vigorous of mycelia length, in the time of 15 days, the bacterium bag has been pinched motionless with hand, when the surface mycelia has purpling red, broken bag is dried;
(5), the special mycelia of slave by drying, wheat mycelia, black bitter buckwheat mycelia be according to wheat 50%, the slave is special 30%, black bitter buckwheat 20% ratio is pulverized with micronizer, more than fineness reaches 150 orders, packing is finished product.
The invention has the beneficial effects as follows: the present invention adopts the slave spy, wheat, black bitter buckwheat dried silkworm chrysalis meal is as culture medium, the packed production of liquid inoculation, its feature is with short production cycle, production cost is low, can realize batch production production, and the slave is extraordinarily thick fiber content 11.5%, crude protein 21.17%, crude fat and isomalto-oligosaccharide 4.17%, can meet the nutrient requirement that Cordyceps Militaris is grown required, do after culture medium with it the content that has improved cordycepin, and its peculiar taste, improve the mycelium powder of sinensis people and be difficult to the mouthfeel of accepting, wheat broth can improve the content of cordycepic acid, black bitter buckwheat is of value to the Chinese caterpillar fungus hypha growth and improves the amino acid in Chinese caterpillar fungus hypha, and other micronutrient levels, dried silkworm chrysalis meal is pulverized high-temperature sterilization after drying and is added, for the growth of Chinese caterpillar fungus hypha provides necessary nutritional labeling, the mycelium powder of sinensis of processing has retained whole active ingredients of wild Chinese caterpillar fungus, there are the Chinese caterpillar fungus various functions useful to human body simultaneously, through chemical examination, cordycepin 2%, cordycepic acid 3% crude fibre 5%, mouthfeel is good, easy to digest, price is low, there is immunological regulation, protect the effect of lung kidney-nourishing.
The specific embodiment
For Comprehension and Implementation better, below explain a kind of mycelium powder of sinensis production technology, comprise the following steps:
A kind of mycelium powder of sinensis production technology comprises the following steps:
(1), make grain culture medium:
(1), select slave special (being commonly called as peach beans, chick-pea), wheat, black bitter buckwheat, dried silkworm chrysalis meal, at first slave spy, wheat, black bitter buckwheat are cleaned up, put into respectively the container steam heated, temperature is controlled at 103 ℃, pressure 0.03 MPa, special 60 minutes of digestion time slave, wheat 20 minutes, black bitter buckwheat 8 minutes;
(2), put fresh silkworm chrysalis into baking oven, temperature is controlled at 95 ℃, dry 8 hours, until water content lower than 2%, temperature is while being down to 23 ℃, with 60 purpose pulverizers, pulverize;
(3), slave spy, wheat, black bitter buckwheat stir with dried silkworm chrysalis meal respectively after boiling, its ratio dried silkworm chrysalis meal respectively accounts for 6%, uses automatic packer to be respectively charged in bag, 1.55 kilograms every bag, then put sterilizing in sterilization tank into, temperature is controlled at 121 ℃, pressure 0.15 MPa, 150 minutes time;
(4), when the sterilizing Pressure Drop to zero the time, open the sterilization tank door, send into the cooling chamber cooling, send into the aseptic inoculation chamber when temperature is down to 20 ℃, wait for inoculation, the inoculation indoor temperature is 20 ℃, positive pressure operation;
(2), make liquid spawn:
(1) worm summer in, seed selection high-quality winter entomogenous fungi kind is female plants;
(2), prepare mother culture media: mother culture media is pressed following weight batching: dried silkworm chrysalis meal 5 g, potato 100 g, wheat bran 200 g, brown sugar 20 g, potassium dihydrogen phosphate 2 g, magnesium sulfate 1 g, agar 18 g, VB
14g, VB
62g puts into water by peeling potatoes stripping and slicing (5mm*5mm) and boils together with dried silkworm chrysalis meal, wheat bran, and slow fire, after 20 minutes, by 4 layers of filtered through gauze, is supplied water to 1000 milliliter, in order by brown sugar, potassium dihydrogen phosphate, magnesium sulfate, agar, VB after filtration
1, VB
6add respectively, the culture medium prepared is distributed into while hot and connects the bacterium box, loading amount height 0.4mm, the diameter 8cm of box, during sterilizing, lid got well and use each wrapping of brown paper tightly to put into the high-pressure sterilizing pot sterilizing, keeping flat, temperature is controlled at 121 ℃, pressure 0.15 MPa, sterilizing 40 minutes, during pot pressure back to zero subject to sterilization, take out and connect the bacterium box, temperature descends, connect in the bacterium box after culture medium solidifying, whether thorough in order to check sterilizing, will connect the bacterium box and be placed under the environment of 35 ℃, cultivate 48 hours, the culture medium connect in the bacterium box can be used without the miscellaneous bacteria bacterium colony;
(3), by the qualified bacterium box that connects, put into superclean bench, opening ultraviolet lamp irradiates 30 minutes, alcohol with 75% is to work top, hand, inoculating tools etc. carry out disinfection, with alcolhol burner to transfer needle, the vaccinating lancet sterilization, then the female water of planting of Chinese caterpillar fungus is cleaned to its surperficial silt, then with cotton ball soaked in alcohol, repeatedly clear up its surface, scrape off the skin on Chinese caterpillar fungus surface with the vaccinating lancet after sterilization, in superclean bench, it is cut to one section by 1 millimeters long, each connects the bacterium box and puts four segments, two sections of polypides, two sections of cursive script, by 3 centimeters of square pitch, put, then put into insulating box, 21 ℃ of temperature, cultivate 6 days, when the little segment length of the polypide connect on culture medium in inoculating box has the white mycelia of one centimeter size of diameter, again it is moved and receives in slant tube, the culture medium of test tube is the same with the culture medium way that connects the bacterium box, while hot the test tube after sterilizing is put into to inclined-plane, put into insulating box after solidifying, temperature is controlled at 21 ℃, cultivate 6 days, treat that the inclined-plane mycelia covers with, plant when producing in a large number and use as mother,
(4), make the shaking flask culture medium: the shaking flask culture medium is according to following weight batching: dried silkworm chrysalis meal 5g, potato 100 g, wheat bran 200 g, brown sugar 20 g, potassium dihydrogen phosphate 2 g, magnesium sulfate 1 g, VB
14 g, VB
62 g, sterilizing is identical with the Mother culture based method, when temperature drops to 21 ℃, send in the clean work station after sterilization mother is planted from test tube and takes out 4 bacterium pieces that area is 5mm*5mm, be placed on the shaking flask liquid level, send 20 ℃ of insulating boxs to, cultivate 88 hours, be placed on shaking table until the bacterium piece when surrounding grows into circle (15mm) and shake, per minute 110 turn, rotating and culturing 72 hours, having mycelium pellet evenly to be formed on can be stand-by in liquid;
(5), make the retort culture medium: the retort culture medium is pressed following weight batching: soya bean 2000g, starch 4000 g, wheat bran 1000 g, potassium dihydrogen phosphate 20 g, magnesium sulfate 10 g, peptone 100 g, soya-bean oil 200 g, the soya bean making beating is filtered, starch is with after cold water dilution (ratio 1:20), being heated to 63 ℃, adding water with soya-bean milk mixing wheat bran boils and boils 15 minutes, after filtering and soya-bean milk starch mix and blend be sequentially added into again potassium dihydrogen phosphate, magnesium sulfate, peptone, after precipitation, do not pour retort into, moisturizing to 200 liter, while heating to 80 ℃, opening intake valve blows 5 minutes, the air foam of generation and impurity are removed, add again soya-bean oil, shut the reaction cover, continue to heat up, when temperature rises to 103 ℃, exhaust once, when temperature reaches 121 ℃, pressure 0.15 MPa, the heat produced in tank, importing and exporting pipeline from respirator passes through, sterilizing 30 minutes, close the respirator terminal valve, keep 121 ℃ of temperature, pressure 0.15 MPa, 40 minutes, start cooling with cooling water, while making temperature be down to 20 ℃, prepare inoculation,
(6), meet bacterial classification mouth place on the top of retort and light the alcohol burning things which may cause a fire disaster, docking shaking flask bacterial classification inoculation pipeline in fire, go out after the connecting source of reducing internal heat is raised shaking flask and is allowed bacterial classification flow into retort, off-response tank inoculation valve, enter normal operating, pressure 0.06 MPa, 21 ℃ of temperature, cultivate 96 hours, the sample examination bacterial classification reaches requirement, prepares to inoculate to grain culture medium;
(3), grain culture medium inoculation: the bacterial classification output channel of retort, with after inoculation device is connected, carries out sterilizing 30 minutes through high temperature of 120 DEG C gas to it, and temperature is down to 22 ℃, starts to inoculate 15 milliliters of every bag of inoculum concentrations;
(4) bag that, connects bacterial classification is sent into long bacterium chamber, 21 ℃ of temperature, submerged fermentation 15 days, turn over layer on the 5th day and check, within the 10th day, the long Bai Shiyong hand of bacterial classification is to every bag of careful pinching, destroy its normal mycelial growth, thereby produce more bacterium head, and make the more vigorous of mycelia length, in the time of 15 days, the bacterium bag has been pinched motionless with hand, when the surface mycelia has purpling red, broken bag is dried;
(5), the special mycelia of slave by drying, wheat mycelia, black bitter buckwheat mycelia be according to wheat 50%, the slave is special 30%, black bitter buckwheat 20% ratio is pulverized with micronizer, more than fineness reaches 150 orders, packing is finished product.
Claims (2)
1. a mycelium powder of sinensis production technology is characterized in that comprising the following steps:
(1), make grain culture medium:
(1), select slave spy, wheat, black bitter buckwheat, dried silkworm chrysalis meal, at first slave spy, wheat, black bitter buckwheat are cleaned up, put into respectively the container steam heated, temperature is controlled at 100 ℃-105 ℃, pressure 0.03-0.05 MPa, the special 60-65 of digestion time slave minute, wheat 20-25 minute, black bitter buckwheat 8-10 minute;
(2), put fresh silkworm chrysalis into baking oven, temperature is controlled at 90 ℃-100 ℃, dries 8-10 hour, until water content lower than 2%, temperature is while being down to 20 ℃-25 ℃, with 60 purpose pulverizers pulverizing;
(3), after boiling, slave spy, wheat, black bitter buckwheat stir with dried silkworm chrysalis meal respectively, its ratio dried silkworm chrysalis meal respectively accounts for 5-7%, use automatic packer to be respectively charged in bag, every bag of 1.5-1.6 kilogram, then put sterilizing in sterilization tank into, temperature is controlled at 120 ℃-122 ℃, pressure 0.13-0.15 MPa, time 130-150 minute;
(4), when the sterilizing Pressure Drop to zero the time, open the sterilization tank door, send into the cooling chamber cooling, send into the aseptic inoculation chamber when temperature is down to 18 ℃-20 ℃, wait for inoculation, the inoculation indoor temperature is 19-21 ℃, positive pressure operation;
(2), make liquid spawn:
(1) worm summer in, seed selection high-quality winter entomogenous fungi kind is female plants;
(2), prepare mother culture media: mother culture media is prepared burden by following weight ratio: dried silkworm chrysalis meal 1.4-1.5%, potato 28-29%, wheat bran 56-57%, brown sugar 5-6%, potassium dihydrogen phosphate 0.5-0.6%, magnesium sulfate 0.2-0.3%, agar 5-6%, VB
11-2%, VB
60.5-0.6%, by the peeling potatoes stripping and slicing, size is boiled for 5mm*5mm puts into water together with dried silkworm chrysalis meal, wheat bran, after slow fire 20-25 minute, by 4 layers of filtered through gauze, supply water to 1000 milliliter after filtration, in order by brown sugar, potassium dihydrogen phosphate, magnesium sulfate, agar, VB
1, VB
6add respectively, the culture medium prepared is distributed into while hot and connects the bacterium box, loading amount height 0.3mm-0.5mm, the diameter 8cm of box, during sterilizing, that lid is good and with brown paper, each wrapping is tightly put into to the high-pressure sterilizing pot sterilizing, keep flat, temperature is controlled at 120 ℃-122 ℃, pressure 0.13-0.15 MPa, sterilizing 35-40 minute, during pot pressure back to zero subject to sterilization, taking-up connects the bacterium box, temperature descends, after connecing the interior culture medium solidifying of bacterium box, whether thorough in order to check sterilizing, to connect the bacterium box and be placed under the environment of 32 ℃-35 ℃, cultivate 45-48 hour, the culture medium connect in the bacterium box can be used without the miscellaneous bacteria bacterium colony,
(3), by the qualified bacterium box that connects, put into superclean bench, open ultraviolet lamp and irradiate 30-35 minute, alcohol with 75% is to work top, hand, inoculating tool carries out disinfection, with alcolhol burner to transfer needle, the vaccinating lancet sterilization, then the female water of planting of Chinese caterpillar fungus is cleaned to its surperficial silt, then with cotton ball soaked in alcohol, repeatedly clear up its surface, scrape off the skin on Chinese caterpillar fungus surface with the vaccinating lancet after sterilization, in superclean bench, it is cut to one section by 1 millimeters long, each connects the bacterium box and puts four segments, two sections of polypides, two sections of cursive script, by 3 centimeters of square pitch, put, then put into insulating box, 20 ℃-22 ℃ of temperature, cultivate 5-7 days, when the little segment length of the polypide connect on culture medium in inoculating box has the white mycelia of one centimeter size of diameter, again it is moved and receives in slant tube, the culture medium of test tube is the same with the culture medium way that connects the bacterium box, while hot the test tube after sterilizing is put into to inclined-plane, put into insulating box after solidifying, temperature is controlled at 20 ℃-22 ℃, cultivate 5-7 days, treat that the inclined-plane mycelia covers with, plant when producing in a large number and use as mother,
(4), make the shaking flask culture medium: the shaking flask culture medium is prepared burden according to following weight ratio: dried silkworm chrysalis meal 1-2%, potato 25-35%, wheat bran 55-65%, brown sugar 5-7%, potassium dihydrogen phosphate 0.5-0.7%, magnesium sulfate 0.2-0.4%, VB
11-2 %, VB
60.3-0.5%, sterilizing is identical with the Mother culture based method, when temperature drops to 19 ℃-22 ℃, sends in the clean work station after sterilization mother is planted from test tube and takes out 4 bacterium pieces that area is 5mm*5mm, be placed on the shaking flask liquid level, send 20 ℃ of insulating boxs to, cultivate 72-96 hour, treat that the bacterium piece grows into circle to surrounding, diameter is placed on shaking table while being 15mm and shakes, 100-120 per minute turns, rotating and culturing 65-72 hour, and having mycelium pellet evenly to be formed on can be stand-by in liquid;
(5), make the retort culture medium: the retort culture medium is prepared burden by following weight ratio: soya bean 27-28%, starch 54-55%, wheat bran 13-14%, potassium dihydrogen phosphate 0.2-0.3%, magnesium sulfate 0.1-0.2%, peptone 1-2%, soya-bean oil 2-3%, the soya bean making beating is filtered, starch dilutes with cold water, the ratio of starch and cold water is to be heated to 60 ℃-65 ℃ after 1:20, adding water with soya-bean milk mixing wheat bran boils and boils 15-20 minute, after filtering and soya-bean milk starch mix and blend be sequentially added into again potassium dihydrogen phosphate, magnesium sulfate, peptone, after precipitation, do not pour retort into, moisturizing to 200 liter, while heating to 80 ℃-85 ℃, open intake valve and blow 5-8 minute, the air foam of generation and impurity are removed, add again soya-bean oil, shut the reaction cover, continue to heat up, when temperature rises to 100 ℃-105 ℃, exhaust once, when temperature reaches 120 ℃-122 ℃, pressure 0.13-0.15 MPa, the heat produced in tank, importing and exporting pipeline from respirator passes through, sterilizing 30-35 minute, close the respirator terminal valve, keep 120 ℃-122 ℃ of temperature, pressure 0.13-0.15 MPa, 40-45 minute, start cooling with cooling water, while making temperature be down to 18 ℃-20 ℃, prepare inoculation,
(6), meet bacterial classification mouth place on the top of retort and light the alcohol burning things which may cause a fire disaster, docking shaking flask bacterial classification inoculation pipeline in fire, go out after the connecting source of reducing internal heat is raised shaking flask and is allowed bacterial classification flow into retort, off-response tank inoculation valve, enter normal operating, pressure 0.05-0.07 MPa, 19 ℃-22 ℃ of temperature, cultivate 90-96 hour, the sample examination bacterial classification reaches requirement, prepares to inoculate to grain culture medium;
(3), grain culture medium inoculation: the bacterial classification output channel of retort is with after inoculation device is connected, and through high temperature of 120 DEG C-122, ℃ gas carries out sterilizing 30-35 minute to it, and temperature is down to 18 ℃-22 ℃, starts to inoculate every bag of inoculum concentration 15-20 milliliter;
(4) bag that, connects bacterial classification is sent into long bacterium chamber, 19 ℃-22 ℃ of temperature, submerged fermentation 15 days, turn over layer on the 5th day and check, within the 10th day, the long Bai Shiyong hand of bacterial classification is to every bag of careful pinching, destroy its normal mycelial growth, thereby produce more bacterium head, and make the more vigorous of mycelia length, in the time of 15 days, the bacterium bag has been pinched motionless with hand, when the surface mycelia has purpling red, broken bag is dried;
(5), the special mycelia of slave by drying, wheat mycelia, black bitter buckwheat mycelia be according to wheat 40-50%, the special 30-40% of slave, black bitter buckwheat 10-20% ratio are pulverized with micronizer, more than fineness reaches 150 orders, packing is finished product.
2. a kind of mycelium powder of sinensis production technology according to claim 1 is characterized in that comprising the following steps:
(1), make grain culture medium:
(1), select slave spy, wheat, black bitter buckwheat, dried silkworm chrysalis meal, at first slave spy, wheat, black bitter buckwheat are cleaned up, put into respectively the container steam heated, temperature is controlled at 103 ℃, pressure 0.03 MPa, special 60 minutes of digestion time slave, wheat 20 minutes, black bitter buckwheat 8 minutes;
(2), put fresh silkworm chrysalis into baking oven, temperature is controlled at 95 ℃, dry 8 hours, until water content lower than 2%, temperature is while being down to 23 ℃, with 60 purpose pulverizers, pulverize;
(3), slave spy, wheat, black bitter buckwheat stir with dried silkworm chrysalis meal respectively after boiling, its ratio dried silkworm chrysalis meal respectively accounts for 6%, uses automatic packer to be respectively charged in bag, 1.55 kilograms every bag, then put sterilizing in sterilization tank into, temperature is controlled at 121 ℃, pressure 0.15 MPa, 150 minutes time;
(4), when the sterilizing Pressure Drop to zero the time, open the sterilization tank door, send into the cooling chamber cooling, send into the aseptic inoculation chamber when temperature is down to 20 ℃, wait for inoculation, the inoculation indoor temperature is 20 ℃, positive pressure operation;
(2), make liquid spawn:
(1) worm summer in, seed selection high-quality winter entomogenous fungi kind is female plants;
(2), prepare mother culture media: mother culture media is prepared burden by following weight ratio: dried silkworm chrysalis meal 1.42%, potato 28.41%, wheat bran 56.82%, brown sugar 5.68%, potassium dihydrogen phosphate 0.57%, magnesium sulfate 0.28%, agar 5.12%, VB
11.14%, VB
60.57%, by the peeling potatoes stripping and slicing, size is boiled for 5mm*5mm puts into water together with dried silkworm chrysalis meal, wheat bran, and slow fire, after 20 minutes, by 4 layers of filtered through gauze, is supplied water to 1000 milliliter after filtration, in order by brown sugar, potassium dihydrogen phosphate, magnesium sulfate, agar, VB
1, VB
6add respectively, the culture medium prepared is distributed into while hot and connects the bacterium box, loading amount height 0.4mm, the diameter 8cm of box, during sterilizing, that lid is good and with brown paper, each wrapping is tightly put into to the high-pressure sterilizing pot sterilizing, keep flat, temperature is controlled at 121 ℃, pressure 0.15 MPa, sterilizing 40 minutes, during pot pressure back to zero subject to sterilization, take out and connect the bacterium box, temperature descends, connect in the bacterium box after culture medium solidifying, whether thorough in order to check sterilizing, will connect the bacterium box and be placed under the environment of 35 ℃, cultivate 48 hours, the culture medium connect in the bacterium box can be used without the miscellaneous bacteria bacterium colony;
(3), by the qualified bacterium box that connects, put into superclean bench, opening ultraviolet lamp irradiates 30 minutes, alcohol with 75% is to work top, hand, inoculating tool carries out disinfection, with alcolhol burner to transfer needle, the vaccinating lancet sterilization, then the female water of planting of Chinese caterpillar fungus is cleaned to its surperficial silt, then with cotton ball soaked in alcohol, repeatedly clear up its surface, scrape off the skin on Chinese caterpillar fungus surface with the vaccinating lancet after sterilization, in superclean bench, it is cut to one section by 1 millimeters long, each connects the bacterium box and puts four segments, two sections of polypides, two sections of cursive script, by 3 centimeters of square pitch, put, then put into insulating box, 21 ℃ of temperature, cultivate 6 days, when the little segment length of the polypide connect on culture medium in inoculating box has the white mycelia of one centimeter size of diameter, again it is moved and receives in slant tube, the culture medium of test tube is the same with the culture medium way that connects the bacterium box, while hot the test tube after sterilizing is put into to inclined-plane, put into insulating box after solidifying, temperature is controlled at 21 ℃, cultivate 6 days, treat that the inclined-plane mycelia covers with, plant when producing in a large number and use as mother,
(4), make the shaking flask culture medium: the shaking flask culture medium is prepared burden according to following weight ratio: dried silkworm chrysalis meal 1.5%, potato 30%, wheat bran 60%, brown sugar 6%, potassium dihydrogen phosphate 0.6%, magnesium sulfate 0.3%, VB
11.2 %, VB
60.4 %, sterilizing is identical with the Mother culture based method, when temperature drops to 21 ℃, sends in the clean work station after sterilization mother is planted from test tube and takes out 4 bacterium pieces that area is 5mm*5mm, be placed on the shaking flask liquid level, send 20 ℃ of insulating boxs to, cultivate 88 hours, treat that the bacterium piece grows into circle to surrounding, diameter is placed on shaking table while being 15mm and shakes, per minute 110 turn, rotating and culturing 72 hours, and having mycelium pellet evenly to be formed on can be stand-by in liquid;
(5), make the retort culture medium: the retort culture medium is prepared burden by following weight ratio: soya bean 27.3%, starch 54.6%, wheat bran 13.6%, potassium dihydrogen phosphate 0.27%, magnesium sulfate 0.14%, peptone 1.4%, soya-bean oil 2.7%, the soya bean making beating is filtered, starch dilutes with cold water, the ratio of starch and cold water is to be heated to 63 ℃ after 1:20, adding water with soya-bean milk mixing wheat bran boils and boils 15 minutes, after filtering and soya-bean milk starch mix and blend be sequentially added into again potassium dihydrogen phosphate, magnesium sulfate, peptone, after precipitation, do not pour retort into, moisturizing to 200 liter, while heating to 80 ℃, opening intake valve blows 5 minutes, the air foam of generation and impurity are removed, add again soya-bean oil, shut the reaction cover, continue to heat up, when temperature rises to 103 ℃, exhaust once, when temperature reaches 121 ℃, pressure 0.15 MPa, the heat produced in tank, importing and exporting pipeline from respirator passes through, sterilizing 30 minutes, close the respirator terminal valve, keep 121 ℃ of temperature, pressure 0.15 MPa, 40 minutes, start cooling with cooling water, while making temperature be down to 20 ℃, prepare inoculation,
(6), meet bacterial classification mouth place on the top of retort and light the alcohol burning things which may cause a fire disaster, docking shaking flask bacterial classification inoculation pipeline in fire, go out after the connecting source of reducing internal heat is raised shaking flask and is allowed bacterial classification flow into retort, off-response tank inoculation valve, enter normal operating, pressure 0.06 MPa, 21 ℃ of temperature, cultivate 96 hours, the sample examination bacterial classification reaches requirement, prepares to inoculate to grain culture medium;
(3), grain culture medium inoculation: the bacterial classification output channel of retort, with after inoculation device is connected, carries out sterilizing 30 minutes through high temperature of 120 DEG C gas to it, and temperature is down to 22 ℃, starts to inoculate 15 milliliters of every bag of inoculum concentrations;
(4) bag that, connects bacterial classification is sent into long bacterium chamber, 21 ℃ of temperature, submerged fermentation 15 days, turn over layer on the 5th day and check, within the 10th day, the long Bai Shiyong hand of bacterial classification is to every bag of careful pinching, destroy its normal mycelial growth, thereby produce more bacterium head, and make the more vigorous of mycelia length, in the time of 15 days, the bacterium bag has been pinched motionless with hand, when the surface mycelia has purpling red, broken bag is dried;
(5), the special mycelia of slave by drying, wheat mycelia, black bitter buckwheat mycelia be according to wheat 50%, the slave is special 30%, black bitter buckwheat 20% ratio is pulverized with micronizer, more than fineness reaches 150 orders, packing is finished product.
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