CN109007823A - Preparation method of selenium-rich cordyceps militaris powder - Google Patents
Preparation method of selenium-rich cordyceps militaris powder Download PDFInfo
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- CN109007823A CN109007823A CN201810903538.1A CN201810903538A CN109007823A CN 109007823 A CN109007823 A CN 109007823A CN 201810903538 A CN201810903538 A CN 201810903538A CN 109007823 A CN109007823 A CN 109007823A
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- 239000011669 selenium Substances 0.000 title claims abstract description 81
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 68
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 68
- 239000000843 powder Substances 0.000 title claims abstract description 64
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 25
- 238000000855 fermentation Methods 0.000 claims abstract description 20
- 230000004151 fermentation Effects 0.000 claims abstract description 20
- 239000007787 solid Substances 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 235000013305 food Nutrition 0.000 claims abstract description 6
- 241000255789 Bombyx mori Species 0.000 claims description 15
- 235000008473 Cardamine hirsuta Nutrition 0.000 claims description 13
- 244000079426 Cardamine hirsuta Species 0.000 claims description 13
- 230000001954 sterilising effect Effects 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims description 11
- 239000002609 medium Substances 0.000 claims description 11
- 235000009566 rice Nutrition 0.000 claims description 11
- 241000894007 species Species 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 235000021329 brown rice Nutrition 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 239000002361 compost Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000009629 microbiological culture Methods 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 235000012015 potatoes Nutrition 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 claims description 3
- 230000002335 preservative effect Effects 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 238000010563 solid-state fermentation Methods 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 2
- 238000010025 steaming Methods 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 3
- 239000010410 layer Substances 0.000 claims 2
- 239000002344 surface layer Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 241000233866 Fungi Species 0.000 abstract description 4
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 235000013325 dietary fiber Nutrition 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 229920001542 oligosaccharide Polymers 0.000 abstract description 2
- 150000002482 oligosaccharides Chemical class 0.000 abstract description 2
- 229920001184 polypeptide Polymers 0.000 abstract description 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- 238000004090 dissolution Methods 0.000 abstract 1
- 229940091258 selenium supplement Drugs 0.000 description 47
- 241000209094 Oryza Species 0.000 description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 235000008708 Morus alba Nutrition 0.000 description 4
- 240000000249 Morus alba Species 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000000306 component Substances 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000002362 mulch Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000011305 Capsella bursa pastoris Nutrition 0.000 description 1
- 240000008867 Capsella bursa-pastoris Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000218231 Moraceae Species 0.000 description 1
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 1
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- -1 each 10g Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- PRKRTJSKTGAVMH-UHFFFAOYSA-N selenic acid;sodium Chemical compound [Na].O[Se](O)(=O)=O PRKRTJSKTGAVMH-UHFFFAOYSA-N 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a preparation method of selenium-rich cordyceps militaris powder, which comprises the following steps: step (1) preparing a cordyceps militaris mother strain; step (2) preparing cordyceps militaris liquid state fermentation strains; step (3), preparing a cordyceps militaris solid fermentation culture medium; step (4), cordyceps militaris culture and mycelium dissolution; step (5) selenium-rich cordyceps militaris powder is prepared; the product can be used for safe addition of organic selenium in food. The invention has the advantages that: 1. the production of the selenium-rich cordyceps militaris powder is completely established on the basis of organic selenium conversion, so that the absolute safety of a selenium source is ensured; 2. the product not only contains rich organic selenium, but also contains functional components of cordyceps militaris, and dietary fibers such as various oligosaccharides and polypeptides degraded by enzyme; 3. the production efficiency of the selenium-rich edible fungus powder is higher than that of the prior common technology.
Description
Technical field
The invention belongs to agricultural product intensive processing technical fields, are related to a kind of preparation method of selenium-enriched cordceps militaris powder.
Background technique
Selenium is very useful to human health, but inorganic se use efficiency is low, strong toxicity.Plant and microorganism have absorption and
Convert the ability that inorganic selenium is organic selenium, the main residue form of the organic selenium being currently known is seleno-amino acids and with seleno
The protein of amino acid residue, certain biologies polysaccharide in sulfuric acid ester structure can also occur seleno reaction, in addition exist
Sulfur-containing amino acid containing non-protein state in liliaceous plant can also occur seleno reaction, also be of concern at present.
Using the selenium-rich characteristic of certain edible mushrooms with significant physiological function come the selenium in enriched medium, and then will be rich
The additive rich in selenium of selenium edible mushroom ultramicronising crushing preparation human food is always the hot spot of food service industry concern.Such as middle promulgated by the State Council
A kind of product formula and its technique CN201710280008.1 rich in organic selenium of bright patent is by the Asia of 200 ~ 300ppm
Selenic acid sodium solution sprays on the mulberries branch powder of crushing, configures culture medium of edible fungus, dries after fruit body of edible fungi harvest
It pulverizes.But the sodium selenite of actually high concentration is harmful, fructification production effect to the growth of edible mushroom
Rate is low.
In plant, tall bottle with spout Hairy Bittercress powder is currently known with strongest selenium rich ability, this is a kind of crucifer,
It is edible.Mulberry leaf are rich in protein, are selenium-enriched plants, can be further enriched in mulberry leaf using selenium-rich mulberry leaf cultivation silkworm
Organic selenium, silkworm chrysalis are the medium components of cordyceps indispensability.
Cordyceps militaris is rare traditional Chinese medicine, rich in functional components such as Cordyceps sinensis polysaccharide, cordycepin, mannitol, and has richness
Collect the ability of selenium.Cordyceps militaris is the fungi few in number that can well grow on rice.
Therefore higher, the full stress-strain selenium conversion all established within the scope of biological tolerance that we have invented a kind of production efficiencys
On the basis of organic selenium food additives.
Summary of the invention
The purpose of the present invention is to provide a kind of preparation methods of selenium-enriched cordceps militaris powder, using Cordyceps militaris to the enrichment energy of selenium
Power is stronger, and can preferably grow on cereal.It is inclined to the characteristic of transformed animal albumen, to be rich in the brown rice of organic selenium
Culture medium of the mulberry silkworm chrysalis powder of powder, tall bottle with spout Hairy Bittercress powder and selenium-rich as Cordyceps militaris is inoculated with after Cordyceps militaris when mycelia covers with training
After supporting container, heating promotes Cordyceps militaris automyophagy, and release protease, cellulase and amylase divide greatly in culture medium
Sub- substance is degraded, the organic selenium of acquisition high-selenium corn efficiency and a large amount of cordycepins.Direct dried bean noodles and crushing can prepare mouthfeel
Good selenium-rich food additive.
The technical solution adopted by the invention is as follows: a kind of preparation method of selenium-enriched cordceps militaris powder, it is characterised in that method step
It is rapid as follows:
The preparation of step (1) Cordyceps militaris parent species；
Activated from Chinese industrial Microbiological Culture Collection administrative center purchase cordyceps militaris link bacterial strain with PDA culture medium, 18-- ~
At 22 DEG C, shading culture；
Take mycelia from the edge of activated dominant colony, is inoculated into domestication culture medium, using domestication slant medium,
At 18-- ~ 22 DEG C, shading culture；
The vigorous mycelium of growing way is selected from domestication culture medium to be inoculated on Mother culture inclined-plane, at 18-- ~ 22 DEG C, shading
It cultivates mycelia and is paved with 3/4 inclined-plane, be put into 4 DEG C of refrigerators, be kept in dark place spare；
The preparation of step (2) Cordyceps militaris liquid state fermentation strain；Dress such as 1200ml fluid nutrient medium, inoculation in the big conical flask of 2000ml
The Cordyceps militaris parent species that 3 main pipes have been tamed, with the smallest shake frequency, at 18- ~ 22 DEG C, shading culture, until shape on shaking table
It can be used to the Inoculant of solid state fermentation at intensive mycelium pellet；
The preparation of step (3) Cordyceps militaris solid fermentation culture medium；Cordyceps militaris solid fermentation culture medium prescription is as follows: organic Se-rich brown rice
40 ~ 50 parts of powder, 20 parts ~ 30 parts of tall bottle with spout Hairy Bittercress powder, 30 parts of the mulberry silkworm chrysalis powder of selenium-rich, 35 ~ 45 parts of clean tap water, are sufficiently mixed
It is uniformly fitted into the stainless steel steamer of 3 layers of sterile gauze of bottom place mat, thickness 4cm, shakeouts 3 layers of sterile gauze of covering, 100 DEG C of steamings
Steam cooker 30min, it is cooling, move in Sterile culture room.
The culture of step (4) Cordyceps militaris and myceliolysis；It in Sterile culture room, requires, raises in strict accordance with aseptic inoculation
Surface mulch gauze, to the solid fermentation inoculation of medium liquid state fermentation strain after sterilizing, inoculum concentration is solid culture base weight
The 15% ~ 20% of amount；Again gauze is covered, at 18-- ~ 22 DEG C, shading culture to mycelia covers culture medium, whole process comprehensively
Continue 3 ~ 5 days；
The sufficient Cordyceps militaris compost that ferments is fitted into the big tank of the ceramics to have sterilized, preservative film sealing moves into sterile processing vehicle
Between in Intelligent culture case, temperature is increased to 45 DEG C, keeps the temperature 4 ~ 6 hours, enzymatic hydrolysis sufficiently after, be transferred in hot hot air drier immediately
Forced air drying is lower than 12% to moisture content at 80 DEG C；
The preparation of step (5) selenium-enriched cordceps militaris powder；
Cordyceps militaris dry fermentation material prepared by step (4) is crossed into 80 ~ 120 mesh nets with beater disintegrating machine；Again with micronizer powder
Broken 200 ~ 300 mesh screen of mistake obtains the ultramicronising selenium-enriched cordceps militaris powder of 400 ~ 700mg/kg of organic selenium content, with 100ml plastics
Bottle or Brown Glass Brown glass bottles and jars only filling and sealing；Product can be used for the safety addition of food organic selenium.
Activation PDA culture medium is formulated as follows in the step (1) of the present invention: peeled potatoes 200g(liquor), grape
Sugared 20g, agar 18g, water 1000ml, pH are natural.
Inclined-plane culture based formulas is as follows in the step (1) of the present invention: organic Se-rich coarse rice powder, tall bottle with spout Hairy Bittercress powder, richness
Mulberry silkworm chrysalis powder, each 10g, glucose 5g, agar 18g, the water 1000ml of selenium, high pressure steam sterilization, pH are natural.
Parent species inclined-plane culture based formulas is as follows in the step (1) of the present invention: organic Se-rich coarse rice powder, tall bottle with spout Hairy Bittercress
Powder, the mulberry silkworm chrysalis powder of selenium-rich, each 15g, agar 18g, water 1000ml, high pressure steam sterilization, pH are natural.
Liquid Culture based formulas is as follows in step (2) of the present invention: organic Se-rich coarse rice powder, tall bottle with spout Hairy Bittercress powder, richness
Mulberry silkworm chrysalis powder, each 20g, MgSO of selenium41.0g、KH2PO41.0g, water 1000ml, high pressure steam sterilization, pH are natural.
Advantages of the present invention: 1, the production of selenium-enriched cordceps militaris powder is all established on the basis of organic selenium conversion, it is ensured that
Selenium source is perfectly safe；2, not only organic selenium rich in while the also functional components containing Cordyceps militaris, enzyme drop in product
The dietary fibers such as the various oligosaccharides, the polypeptide that solve；3, the production efficiency of selenium-enriched edible mushroom powder is higher than current general technology.
Specific embodiment
Embodiment 1
One kind includes: with selenium-enriched cordceps militaris powder producing method, main technological steps
Step 1: prepared by Cordyceps militaris parent species；
Cordyceps militaris link bacterial strain CICC14013 is bought from Chinese industrial Microbiological Culture Collection administrative center, is lived with PDA culture medium
Change.It is formulated using activation PDA culture medium as follows: peeled potatoes 200g(liquor), glucose 20g, agar 18g, water
1000ml, pH are natural.At 18 DEG C, shading culture.
Take mycelia from the edge of activated dominant colony, is inoculated into domestication culture medium.It is trained using domestication with inclined-plane
Support base, slant medium: organic Se-rich coarse rice powder, tall bottle with spout Hairy Bittercress powder, the mulberry silkworm chrysalis powder of selenium-rich, each 10g, glucose 5g, fine jade
Rouge 18g, water 1000ml, high pressure steam sterilization, pH nature.At 18 DEG C, shading culture.
It selects the vigorous mycelium of growing way from domestication culture medium to be inoculated on Mother culture inclined-plane, parent species inclined-plane culture
Base: organic Se-rich coarse rice powder, tall bottle with spout Hairy Bittercress powder, the mulberry silkworm chrysalis powder of selenium-rich, each 15g, agar 18g, water 1000ml, high pressure is steamed
Vapour sterilizing, pH are natural.At 18 DEG C, shading culture to mycelia is paved with 3/4 inclined-plane, is put into 4 DEG C of refrigerators, is kept in dark place spare.
Step 2: prepared by Cordyceps militaris liquid state fermentation strain, the dress such as 1200ml fluid nutrient medium in the big conical flask of 2000ml,
The Cordyceps militaris parent species that 3 main pipes have been tamed are inoculated with, with the smallest shake frequency, at 18 DEG C, shading culture, until shape on shaking table
It can be used to the Inoculant of solid state fermentation at intensive mycelium pellet.Liquid Culture based formulas: organic Se-rich coarse rice powder, tall bottle with spout are cracked rice
Shepherd's purse powder, the mulberry silkworm chrysalis powder of selenium-rich, each 20g, MgSO41.0g、KH2PO41.0g, water 1000ml, high pressure steam sterilization, pH are natural.
Step 3: prepared by Cordyceps militaris solid fermentation culture medium；Cordyceps militaris solid fermentation culture medium prescription: organic Se-rich brown rice
40 parts of powder, 30 parts of tall bottle with spout Hairy Bittercress powder, 30 parts of the mulberry silkworm chrysalis powder of selenium-rich, 35 parts of clean tap water are sufficiently mixed and are uniformly packed into bottom
In the stainless steel steamer of 3 layers of sterile gauze of portion's place mat, thickness 4cm shakeouts 3 layers of sterile gauze of covering, 100 DEG C of vapour cookings
30min, it is cooling, move in Sterile culture room.
Step 4: Cordyceps militaris culture and myceliolysis；It in Sterile culture room, requires, raises in strict accordance with aseptic inoculation
Surface mulch gauze, to the solid fermentation inoculation of medium liquid state fermentation strain after sterilizing, inoculum concentration is solid culture base weight
The 20% of amount.Again gauze is covered.At 18 DEG C, shading culture to mycelia covers culture medium comprehensively, and whole process continues 5 days.
The sufficient Cordyceps militaris compost that ferments is fitted into the big tank of the ceramics to have sterilized, preservative film sealing moves into sterile add
In the Intelligent culture case of work workshop, temperature is increased to 45 DEG C, keeps the temperature 6 hours, after enzymatic hydrolysis is abundant, is transferred to hot hot air drier immediately
In at 80 DEG C forced air drying to moisture content be lower than 12%.
Step 5: prepared by selenium-enriched cordceps militaris powder, by the Cordyceps militaris dry fermentation material of step 4 preparation with beater disintegrating machine mistake
100 mesh nets；300 mesh screens crushed with micronizer again, obtain the ultramicronising selenium-rich pupa worm of organic selenium content 600mg/kg
Grass meal, with 100ml plastic bottle or Brown Glass Brown glass bottles and jars only filling and sealing.Product can be used for the safety addition of food organic selenium.
Claims (5)
1. a kind of preparation method of selenium-enriched cordceps militaris powder, it is characterised in that method and step is as follows:
The preparation of step (1) Cordyceps militaris parent species；
Activated from Chinese industrial Microbiological Culture Collection administrative center purchase cordyceps militaris link bacterial strain with PDA culture medium, 18-- ~
At 22 DEG C, shading culture；
Take mycelia from the edge of activated dominant colony, is inoculated into domestication culture medium, using domestication slant medium,
At 18-- ~ 22 DEG C, shading culture；
The vigorous mycelium of growing way is selected from domestication culture medium to be inoculated on Mother culture inclined-plane, at 18-- ~ 22 DEG C, shading
It cultivates mycelia and is paved with 3/4 inclined-plane, be put into 4 DEG C of refrigerators, be kept in dark place spare；
The preparation of step (2) Cordyceps militaris liquid state fermentation strain；Dress such as 1200ml fluid nutrient medium, inoculation in the big conical flask of 2000ml
The Cordyceps militaris parent species that 3 main pipes have been tamed, with the smallest shake frequency, at 18- ~ 22 DEG C, shading culture, until shape on shaking table
It can be used to the Inoculant of solid state fermentation at intensive mycelium pellet；
The preparation of step (3) Cordyceps militaris solid fermentation culture medium；Cordyceps militaris solid fermentation culture medium prescription is as follows: organic Se-rich brown rice
40 ~ 50 parts of powder, 20 parts ~ 30 parts of tall bottle with spout Hairy Bittercress powder, 30 parts of the mulberry silkworm chrysalis powder of selenium-rich, 35 ~ 45 parts of clean tap water, are sufficiently mixed
It is uniformly fitted into the stainless steel steamer of 3 layers of sterile gauze of bottom place mat, thickness 4cm, shakeouts 3 layers of sterile gauze of covering, 100 DEG C of steamings
Steam cooker 30min, it is cooling, move in Sterile culture room；
The culture of step (4) Cordyceps militaris and myceliolysis；It in Sterile culture room, is required in strict accordance with aseptic inoculation, raises surface layer
Cover gauze, to the solid fermentation inoculation of medium liquid state fermentation strain after sterilizing, inoculum concentration is solid medium weight
15%~20%；Again gauze is covered, at 18-- ~ 22 DEG C, shading culture to mycelia covers culture medium comprehensively, and whole process continues 3
~ 5 days；
The sufficient Cordyceps militaris compost that ferments is fitted into the big tank of the ceramics to have sterilized, preservative film sealing moves into sterile processing vehicle
Between in Intelligent culture case, temperature is increased to 45 DEG C, keeps the temperature 4 ~ 6 hours, enzymatic hydrolysis sufficiently after, be transferred in hot hot air drier immediately
Forced air drying is lower than 12% to moisture content at 80 DEG C；
The preparation of step (5) selenium-enriched cordceps militaris powder；
Cordyceps militaris dry fermentation material prepared by step (4) is crossed into 80 ~ 120 mesh nets with beater disintegrating machine；Again with micronizer powder
Broken 200 ~ 300 mesh screen of mistake obtains the ultramicronising selenium-enriched cordceps militaris powder of 400 ~ 700mg/kg of organic selenium content, with 100ml plastics
Bottle or Brown Glass Brown glass bottles and jars only filling and sealing；Product can be used for the safety addition of food organic selenium.
2. activation PDA culture medium is formulated as follows in the step (1) of the present invention: peeled potatoes 200g(liquor), glucose
20g, agar 18g, water 1000ml, pH are natural.
3. a kind of preparation method of selenium-enriched cordceps militaris powder according to claim 1, it is characterised in that: in the step (1)
Inclined-plane culture based formulas is as follows: organic Se-rich coarse rice powder, tall bottle with spout Hairy Bittercress powder, the mulberry silkworm chrysalis powder of selenium-rich, each 10g, glucose 5g,
Agar 18g, water 1000ml, high pressure steam sterilization, pH are natural.
4. a kind of preparation method of selenium-enriched cordceps militaris powder according to claim 1, it is characterised in that: in the step (1)
Parent species inclined-plane culture based formulas is as follows: organic Se-rich coarse rice powder, tall bottle with spout Hairy Bittercress powder, the mulberry silkworm chrysalis powder of selenium-rich, each 15g, agar
18g, water 1000ml, high pressure steam sterilization, pH are natural.
5. a kind of preparation method of selenium-enriched cordceps militaris powder according to claim 1, it is characterised in that: in the step (2)
Liquid Culture based formulas is as follows: organic Se-rich coarse rice powder, tall bottle with spout Hairy Bittercress powder, the mulberry silkworm chrysalis powder of selenium-rich, each 20g,
MgSO41.0g、KH2PO41.0g, water 1000ml, high pressure steam sterilization, pH are natural.
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| CN112899169A (en) * | 2021-01-26 | 2021-06-04 | 苏州市十颐健康科技股份有限公司 | Fermentation method of mulberry leaf cordyceps militaris nano-selenium thallus |
| CN113583880A (en) * | 2021-08-31 | 2021-11-02 | 扬州大学 | Culture medium suitable for preparing generalized cordyceps sinensis liquid fermentation seed liquid and preparation method and culture method thereof |
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