CN106631344A - Lucid ganoderma bag cultivation culture medium capable of increasing content of ganoderan and selenium - Google Patents
Lucid ganoderma bag cultivation culture medium capable of increasing content of ganoderan and selenium Download PDFInfo
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- CN106631344A CN106631344A CN201610869989.9A CN201610869989A CN106631344A CN 106631344 A CN106631344 A CN 106631344A CN 201610869989 A CN201610869989 A CN 201610869989A CN 106631344 A CN106631344 A CN 106631344A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a lucid ganoderma bag cultivation culture medium capable of increasing the content of ganoderan and selenium. The lucid ganoderma bag cultivation culture medium comprises, by weight, 75 parts of wood flour, 20 parts of wheat bran, 2 parts of corn flour, 1 part of lime, 1 part of gypsum and 1 part of sugar. A selenium-enriched nutrient solution is used for adjusting the ratio of the materials to water to be 1: (1.3-1.5). The selenium-enriched nutrient solution is obtained according to a preparation method including: (1), preparing 100 parts of purified water, 1.0 part of L-selenium methionine and 5 parts of corn flour; (2), mixing the raw materials in the step (1), putting the mixture into a high-pressure tank, increasing the pressure to 4kg/cm2 to even the pressure, keeping the pressure for 2-3 minutes, recovering the normal pressure within 1-3 seconds to obtain size, inoculating the size with mature liquid bacillus subtilis cultures by volume percent of 10% at the temperature of 25 DEG C, putting the size into a fermentation tank, injecting sterile air into the fermentation tank, stirring the fermentation tank at the speed of 250r/min, performing fermentation at the temperature of 35-37 DEG C for 4 hours, increasing the temperature to 70 DEG C quickly after fermentation, and keeping 1.0 hour of inactivation to obtain the lucid ganoderma bag cultivation culture medium.
Description
Technical field
The present invention relates to fungus growing technique field, more particularly to a kind of glossy ganoderma that can improve GL-B and Se content
Bag cultivating supports base.
Background technology
Glossy ganoderma (scientific name：Ganoderma Lucidum Karst), also known as polyporus lucidus, celestial grass, another name red sesame, red sesame, wood spirit
Sesame, Ganoderma, 10000 years gill fungus, polyporus lucidus, are the complete stools of Polyporaceae plant red sesame or purple sesame, are the son of On Polyporaceae glossy ganoderma
Entity.Profile is in umbrella, cap kidney shape, semicircle or subcircular.Glossy ganoderma is used as the Chinese tradition for possessing thousands of years medicinal history
Valuable ingredient of traditional Chinese medicine, with invigorating qi for tranquilization, relieving cough and asthma effect, for dizziness egersis, palpitation, consumptive disease cough and asthma.Glossy ganoderma possesses
Very high medical value, through scientific research institution's many decades modern pharmacology research confirm, glossy ganoderma for strengthen body immunity,
Blood sugar is adjusted, blood pressure is controlled, adjuvant therapy chemicotherapy, liver protecting promotes the aspects such as sleep to be respectively provided with significant curative effect.Glossy ganoderma is more
Sugar is one of principle active component of glossy ganoderma medicinal function, is primarily present in glossy ganoderma cell wall inwall, and major part is beta glucan,
Its monose composition in addition to containing a large amount of glucose, also containing a small amount of arabinose, wood sugar, fucose, rhamnose, galactolipin and sweet
Dew sugar etc., they are mostly bonded with glucosides such as (1-3), (1-4) and (1-6), with extensive biologically active, such as strengthen immunity
The effect such as power, removing human free radical, protection angiocarpy, radioresistance, protection liver, antitumor.Triterpenes is also having for glossy ganoderma
Effect one of composition, triterpenes is no less than 100 kinds of contained by glossy ganoderma, wherein based on tetracyclic triterpenes, the bitter taste of glossy ganoderma and contained triterpene
Class is relevant, has CDCC to human hepatoma cell, can also suppress the release of histamine, makees with liver protection and antiallergy etc.
With.
At present, the technology such as artificial generation (bottle) material cultivation, fermenting and producing of glossy ganoderma is in succession successful, wherein, fermenting and producing
High to equipment requirement, investment is big, is unfavorable for wide popularization and application；Ganoderma Lucidum is long-standing in China, from cultivation basswood
To the development of substituting stuff cultivation technology, glossy ganoderma artificial cultivation on a large scale is promoted.Such as Chinese patent CN103724115A, one is disclosed
Lucid ganoderma pocket planting fungus bag is planted, is mainly formulated using following raw materials by weight：Mulberry wood chips 75-80%, cotton seed hulls 10-
16%th, wheat bran 3-5%, land plaster 0.8-1.2%, water content 55-65%, pH value 6.5-7.5, pack.Required for glossy ganoderma growth
Nutriment and other edible mushrooms on the whole for unanimous on the whole, such as carbon nutrition, nitrogen nutrition, vitamin and mineral matter
Deng in the proportioning of glossy ganoderma substituting stuff cultivation culture medium, traditional major ingredient has cotton seed hulls, weed tree sawdust, agricultural crop straw etc., auxiliary material
There are wheat bran, wooden chaff, corn flour etc., separately there are the additives such as gypsum, calcium superphosphate, white sugar.Artificial substituting stuff cultivation is mainly with wood chip, cotton
The agricultural such as seed shell, bagasse, crop material leftover bits and pieces and waste residue waste material make culturing raw material, easy to operate, small investment, it is easy to generally push away
Wide application, but waste residue waste material complicated component, some possibility contain harmful components, unfavorable to ganoderma lucidum product quality control.
Selenium is trace element needed by human.Selenium participates in various selenoenzymes and Selenonic protein in synthesized human.Wherein paddy Guang
Sweet peptide peroxidase, is catalyzed hydroperoxides or MDA is changed into water or various alcohols in vivo, eliminates
Free radical protects biomembrane from oxidative damage to biomembranous attack；Selenium participates in constituting the de- iodine enzyme of iodate thyroid gland amino acid.
In vivo selenium and vitamin E collaboration, can protect cell membrane, prevent the oxidation of unrighted acid.Trace selenium has protective effect on cancer risk
And liver-protective effect.Selenium can improve human immunity, promote the propagation of lymphocyte and the synthesis of antibody and immunoglobulin (Ig).
Selenium has the effect for significantly suppressing and protecting to kinds cancers such as colon cancer, cutaneum carcinoma, liver cancer, breast cancer, and it is in body
Mesostate methyl enol have stronger active anticancer.The battalion such as selenium and vitamin E, allicin, linoleic acid, germanium, zinc
Support element and there is the oxidation resistant effect of collaboration, increase antioxidation activity.Meanwhile, selenium has the work for mitigating and alleviating heavy metal toxicity
With.
But, naturally the glossy ganoderma quantity of growth is few, and containing substantial amounts of crude fibre and other impurity, and GL-B contains
Measure not high (usually 2-5%), be not easy to directly as health food or pharmaceutical raw material, therefore, development can improve GL-B
With the key that the artificial culture and cultivation technique research of the glossy ganoderma of Se content are cultivation of glossy ganoderma industrialization.
The content of the invention
It is an object of the invention to provide a kind of Lucid ganoderma pocket planting culture medium that can improve GL-B and Se content, can be effective
The content of main active GL-B and selenium in raising glossy ganoderma.
A kind of Lucid ganoderma pocket planting culture medium that can improve GL-B and Se content of the present invention, is by following weight parts
Raw material make：75 parts of wood chip, 20 parts of wheat bran, 2 parts of corn flour, 1 part of lime, 1 part of gypsum, 1 part of sugar, are adjusted using Selenium-rich nutrient solution
Section material-water ratio is 1：1.3-1.5；
Described Selenium-rich nutrient solution, is prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 1.0 parts of L- selenomethionines, 5 parts of corn flour；
2. after the raw material by step 1. mixes, in being put into pressure pan, it is forced into 4kg/cm2So as to pressure is uniform, and keeps
2-3min, then recovers normal pressure in 1-3s, obtains slurries, is the withered of 10% access culture maturation by percent by volume at 25 DEG C
Careless bacillus liquid bacterial classification, in being put into fermentation tank, is passed through filtrated air in fermentation tank, be stirred fermentation, speed of agitator
250r/min, fermentation time is 4h, and temperature is kept for 35-37 DEG C, after fermentation ends, rapidly temperature is risen to into 70 DEG C and keeps 1.0h to go out
It is living, obtain.
The cultural method of the bacillus subtilis liquid spawn is：Using triangular flask strain cultivation, below attach most importance to
Amount percentage, by albumen slurry 30.0-35.0% for processing, KH2P041.0%, MgS042.0%, NaCl0.5% are put into three
In the bottle of angle, stir, adjust pH to 5.6,125 DEG C of sterilizing 10min are cooled to 25 DEG C, access the withered grass through inclined-plane culture
Bacillus, 28 DEG C of shaking table cultures 60-100h, for fermentation tank inoculation.
The preparation method of culture medium of the present invention, including spice, pack, sterilizing etc., with reference to the method for prior art.
Compared with prior art, the invention has the advantages that：
1st, Selenium-rich nutrient solution of the prior art, usually using inorganic selenium, particularly sodium selenite and sodium selenate, sub- selenium
Sour potassium and potassium selenate etc., obtain from the byproduct of metal mineral reserves, and inorganic selenium has larger toxicity, and are difficult to be absorbed, as
Nutrient solution, assimilation effect is bad.The selenomethionine that the present invention is used, is a kind of organic selenium source, compared with inorganic selenium, selenomethionine
With safe and efficient advantage, it can substitute some methionine and be attached to one reversible selenium depots of formation on body protein,
And there is no toxic hazard.
2nd, selenomethionine is that selenium is present in a kind of seleno amino in plant and feed grain with organic form in nature
The plant growths such as acid, cereal and forage crop are not necessarily required to selenomethionine, but with methionine synthesis they can utilize ring
The selenite and selenate synthesis selenomethionine being particularly in border in soil.It is more using selenium in soil, in plant
Selenomethionine content is higher.At present, selenomethionine is mainly derived from natural selenium yeast, is that Organic Selenium is primarily present in selenium yeast
Form, but selenomethionine has dense yeast taste, directly water-soluble rear sprinkling, and absorption efficiency is low and influences whether finally to produce
The taste and flavor of product.Bacillus subtillis, is one kind of Bacillus, and bacillus subtilis thalline itself synthesizes alphalise starch
The enzymes such as enzyme, protease, lipase, cellulase, equivalent to complex enzyme, the present invention is by selenomethionine mixing Ko subtilis bar
Bacterium after fermentation, the zymasis carried using selenomethionine product is fermented, and on the one hand enters glossy ganoderma beneficial to selenium element, on the other hand
With methionine synthesis they can utilize in environment selenite and selenate synthesis selenomethionine in particularly soil, also
Can be effectively improved, dissolve yeast flavor.
3rd, the HIGH PRESSURE TREATMENT that the food sterilization stage uses is applied to the compounding stage by the present invention, by " being forced into
4kg/cm2So as to pressure is uniform, and keeps 2-3min, and normal pressure is then recovered in 1-3s ", pressure is reduced to into 0 by moment,
On the one hand can change to sterilize by the moment of pressure, on the other hand can effectively refine, disperse selenomethionine, advantageously
In the absorption of glossy ganoderma.
Specific embodiment
Below with embodiment, the invention will be further described, but the invention is not limited in these embodiments.
Embodiment 1-5：
In order to verify the technique effect of the present invention, 2016, the product of 5 batches is produced using the technology of the present invention, each
The method of batch is as follows:
1) preparation of culture medium：It is made up of the raw material of following weight parts：Wood chip 75kg, wheat bran 20kg, corn flour 2kg, stone
Grey 1kg, gypsum 1kg, sugar 1kg, adopt Selenium-rich nutrient solution to adjust material-water ratio for 1：1.3-1.5；
2) spice, pack, sterilizing, inoculation；
3) bacterium is sent out：After inoculation, it is put into booth and sends out bacterium, control greenhouse temperature is at 26-28 DEG C；
4) sesame is gone out：After the long purseful of mycelia, glossy ganoderma enters the sesame phase, controls greenhouse temperature to 26-28 DEG C；
Step 1) described in Selenium-rich nutrient solution, be prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 1.0 parts of L- selenomethionines, 5 parts of corn flour；
2. after the raw material by step 1. mixes, in being put into pressure pan, it is forced into 4kg/cm2So as to pressure is uniform, and keeps
2-3min, then recovers normal pressure in 1-3s, obtains slurries, is the withered of 10% access culture maturation by percent by volume at 25 DEG C
Careless bacillus liquid bacterial classification, in being put into fermentation tank, is passed through filtrated air in fermentation tank, be stirred fermentation, speed of agitator
250r/min, fermentation time is 4h, and temperature is kept for 35-37 DEG C, after fermentation ends, rapidly temperature is risen to into 70 DEG C and keeps 1.0h to go out
It is living, obtain.
Comparative example 1：
1) preparation of culture medium：It is made up of the raw material of following weight parts：Wood chip 75kg, wheat bran 20kg, corn flour 2kg, stone
Grey 1kg, gypsum 1kg, sugar 1kg, adopt Selenium-rich nutrient solution to adjust material-water ratio for 1：1.3-1.5；
2) spice, pack, sterilizing, inoculation；
3) bacterium is sent out：After inoculation, it is put into booth and sends out bacterium, control greenhouse temperature is at 26-28 DEG C；
4) sesame is gone out：After the long purseful of mycelia, glossy ganoderma enters the sesame phase, controls greenhouse temperature to 26-28 DEG C；
Step 1) described in Selenium-rich nutrient solution, be prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 1.0 parts of sodium selenite, 5 parts of corn flour；
2. after the raw material by step 1. mixes, in being put into pressure pan, it is forced into 4kg/cm2So as to pressure is uniform, and keeps
2-3min, then recovers normal pressure in 1-3s, obtains slurries, is the withered of 10% access culture maturation by percent by volume at 25 DEG C
Careless bacillus liquid bacterial classification, in being put into fermentation tank, is passed through filtrated air in fermentation tank, be stirred fermentation, speed of agitator
250r/min, fermentation time is 4h, and temperature is kept for 35-37 DEG C, after fermentation ends, rapidly temperature is risen to into 70 DEG C and keeps 1.0h to go out
It is living, obtain.
Comparative example 2：
1) preparation of culture medium：It is made up of the raw material of following weight parts：Wood chip 75kg, wheat bran 20kg, corn flour 2kg, stone
Grey 1kg, gypsum 1kg, sugar 1kg, material-water ratio is 1：1.3-1.5；
2) spice, pack, sterilizing, inoculation；
3) bacterium is sent out：After inoculation, it is put into booth and sends out bacterium, control greenhouse temperature is at 26-28 DEG C；
4) sesame is gone out：After the long purseful of mycelia, glossy ganoderma enters the sesame phase, controls greenhouse temperature to 26-28 DEG C.
As a result：Each batch random sampling, after testing, as a result see the table below：
Claims (2)
1. a kind of Lucid ganoderma pocket planting culture medium that can improve GL-B and Se content, it is characterised in that by the original of following weight parts
Material is made：
75 parts of wood chip, 20 parts of wheat bran, 2 parts of corn flour, 1 part of lime, 1 part of gypsum, 1 part of sugar, using Selenium-rich nutrient solution material water is adjusted
Than for 1：1.3-1.5；
Described Selenium-rich nutrient solution, is prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 1.0 parts of L- selenomethionines, 5 parts of corn flour；
2. after the raw material by step 1. mixes, in being put into pressure pan, it is forced into 4kg/cm2So as to pressure is uniform, and keeps 2-
3min, then recovers normal pressure in 1-3s, obtains slurries, is the ripe withered grass of 10% access culture by percent by volume at 25 DEG C
Bacillus liquid bacterial classification, in being put into fermentation tank, is passed through filtrated air in fermentation tank, be stirred fermentation, speed of agitator 250r/
Min, fermentation time is 4h, and temperature is kept for 35-37 DEG C, after fermentation ends, rapidly temperature is risen to into 70 DEG C and keeps 1.0h inactivations, is obtained
Arrive.
2. a kind of Lucid ganoderma pocket planting culture medium that can improve GL-B and Se content according to claim 1, its feature exists
In：The cultural method of the 2. described bacillus subtilis liquid spawn of step is：Using triangular flask strain cultivation, below for
Percetage by weight, by albumen slurry 30.0-35.0% for processing, KH2PO41.0%, MgSO42.0%, NaCl0.5% are put
In entering triangular flask, stir, adjust pH to 5.6,125 DEG C of sterilizing 10min are cooled to 25 DEG C, access through inclined-plane culture
Bacillus subtilis strain, 28 DEG C of shaking table cultures 60-100h, for fermentation tank inoculation.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646531A (en) * | 2017-09-30 | 2018-02-02 | 安徽利民生物科技股份有限公司 | A kind of preparation method of Se-rich lucid ganoderma culture medium |
| CN108307931A (en) * | 2018-03-31 | 2018-07-24 | 广西陆川和合生物科技有限公司 | A kind of Se-rich lucid ganoderma culture material and preparation method thereof |
| CN108617400A (en) * | 2018-03-31 | 2018-10-09 | 广西陆川和合生物科技有限公司 | A kind of efficient cultivation method of Se-rich lucid ganoderma |
| CN113767812A (en) * | 2021-09-23 | 2021-12-10 | 山西农业大学山西功能食品研究院 | Ganoderma strain and culture method of ganoderma lucidum fruiting body |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107646531A (en) * | 2017-09-30 | 2018-02-02 | 安徽利民生物科技股份有限公司 | A kind of preparation method of Se-rich lucid ganoderma culture medium |
| CN108307931A (en) * | 2018-03-31 | 2018-07-24 | 广西陆川和合生物科技有限公司 | A kind of Se-rich lucid ganoderma culture material and preparation method thereof |
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| CN113767812A (en) * | 2021-09-23 | 2021-12-10 | 山西农业大学山西功能食品研究院 | Ganoderma strain and culture method of ganoderma lucidum fruiting body |
| CN113767812B (en) * | 2021-09-23 | 2023-04-25 | 山西农业大学山西功能食品研究院 | Ganoderma lucidum strain and method for culturing red-meat ganoderma lucidum fruiting body |
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Application publication date: 20170510 |