CN106478236A - The Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved - Google Patents

The Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved Download PDF

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Publication number
CN106478236A
CN106478236A CN201610869594.9A CN201610869594A CN106478236A CN 106478236 A CN106478236 A CN 106478236A CN 201610869594 A CN201610869594 A CN 201610869594A CN 106478236 A CN106478236 A CN 106478236A
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parts
raw
selenium
fermentation
ganoderan
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CN201610869594.9A
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苏华
陆兵
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Huai-An Natural Health-Care Food Development Co Ltd Guilin
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Huai-An Natural Health-Care Food Development Co Ltd Guilin
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention discloses the Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved, it is made up of the raw material of following weight parts:75 parts of wood flour, 20 parts of Testa Tritici, 2 parts of Semen Maydis powder, 1 part of Calx, 1 part of Gypsum Fibrosum, 1 part of sugar, adjusting material-water ratio using Selenium-rich nutrient solution is 1:1.3‑1.5;Described Selenium-rich nutrient solution, is prepared by the following preparation method:1. raw material prepares:Prepare 100 parts of pure water, 1.0 parts of L Selenomethionine, 5 parts of Semen Maydis powder;2., after raw material 1. mixes by step, steam explosion tank, pressure 4MPa are put into, it is heated to 150 DEG C, keep 2min, then quick release pressure obtains serosity in 1 3s, at 25 DEG C by the bacillus subtilises liquid spawn that percent by volume is that maturation is cultivated in 10% access, put in fermentation tank, in fermentation tank, be passed through filtrated air, it is stirred fermenting, speed of agitator 250r/min, fermentation time is 4h, temperature keeps 35 37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keep 1.0h inactivation, obtain.

Description

The Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved
Technical field
The present invention relates to fungus growing technique field, the Lucid ganoderma pocket planting of ganoderan and Se content more particularly, to can be improved Culture medium.
Background technology
Ganoderma (scientific name:Ganoderma Lucidum Karst), also known as Ganoderma lucidum seu Japonicum, Herba mesonae chinensis, the Ganoderma lucidum (Leyss. Ex Fr.) Karst. that has another name called, red sesame, wood spirit Sesame, Ganoderma, Ganoderma lucisum, Ganoderma lucidum seu Japonicum, are the Herbs of Polyporaceae plant Ganoderma lucidum (Leyss. Ex Fr.) Karst. or Ganoderma, are the son of On Polyporaceae Ganoderma Entity.Profile is in umbrella, cap kidney shape, semicircle or subcircular.Ganoderma is as the Chinese tradition having thousands of years medicinal history Valuable ingredient of Chinese medicine, has invigorating QI and tranquilization, effect of relieving cough and asthma, for dizziness sleeplessness, shortness of breath and palpitation, asthenia cough with asthma.Ganoderma possesses Very high medical value, through scientific research institution's many decades modern pharmacology research confirm, Ganoderma for strengthen body immunity, Adjust blood glucose, control blood pressure, adjuvant therapy chemicotherapy, hepatoprotective, the aspect such as hypnotic is respectively provided with significant curative effect.Ganoderma is many Sugar is one of principle active component of glossy ganoderma medicinal function, is primarily present in glossy ganoderma cell wall inwall, and most of is beta glucan, Its monosaccharide forms in addition to containing a large amount of glucoses, also contains a small amount of arabinose, xylose, fucose, rhamnose, galactose and sweet Dew sugar etc., they are mostly bonded with glucosides such as (1-3), (1-4) and (1-6), have extensive biological activity, such as strengthen immunity The effects such as power, removing human free radical, protection cardiovascular, radioprotective, the liver protecting, antitumor.Triterpeness are also having of Ganoderma Effect one of composition, contained by Ganoderma, triterpeness are no less than 100 kinds of, wherein based on tetracyclic triterpenes, the bitterness of Ganoderma and contained triterpene Class is relevant, has cytotoxicity to human hepatoma cell, also can suppress the release of histamine, has liver protection and antiallergic etc. and makees With.
At present, the technology such as artificial generation (bottle) the material cultivation of Ganoderma, fermenting and producing is in succession successful, wherein, fermenting and producing High to equipment requirements, investment is big, is unfavorable for wide popularization and application;Ganoderma Lucidum is long-standing in China, from cultivation basswood To the development of substituting stuff cultivation technology, promote Ganoderma artificial culture on a large scale.As Chinese patent CN103724115A, disclose one Plant Lucid ganoderma pocket planting fungus bag, be mainly formulated using following raw materials by weight:Mulberry wood chips 75-80%, cotton seed hullss 10- 16%th, Testa Tritici 3-5%, Gypsum Fibrosum powder 0.8-1.2%, water content 55-65%, pH value 6.5-7.5, pack.Required for Ganoderma growth Nutrient substance and other edible fungi on the whole for unanimous on the whole, such as carbon nutrition, nitrogen nutrition, vitamin and mineral Deng in the proportioning of Ganoderma substituting stuff cultivation culture medium, traditional major ingredient has cotton seed hullss, weed tree sawdust, agricultural crop straw etc., adjuvant There are Testa Tritici, wooden bran, Semen Maydis powder etc., separately have the additives such as Gypsum Fibrosum, calcium superphosphate, white sugar.Artificial substituting stuff cultivation is mainly with wood flour, cotton The agricultural leftover bits and pieces such as seed shell, bagasse, crop material and waste residue waste material make culturing raw material, easy and simple to handle, and small investment is it is easy to generally push away Wide application, but waste residue waste material complicated component, have may contain harmful components, unfavorable to ganoderma lucidum product quality control.
Selenium is trace element needed by human.Selenium participates in multiple selenoenzymes and Selenonic protein in synthesized human.Wherein paddy Guang Sweet peptide peroxidase, is catalyzed hydroperoxides in vivo or lipid peroxide is changed into water or various alcohols, eliminates Free radical, to biomembranous attack, protects biomembrane from oxidative damage;Selenium participates in constituting the de- iodine enzyme of iodate thyroid amino acid.? Internal selenium and Vitamin E are worked in coordination with, and can protect cell membrane, prevent the oxidation of unsaturated fatty acid.Trace selenium has protective effect on cancer risk And liver-protective effect.Selenium can improve human immunity, promotes the propagation of lymphocyte and the synthesis of antibody and immunoglobulin. Selenium has the obvious effect suppressing and protecting to kinds cancers such as colon cancer, skin carcinoma, hepatocarcinoma, breast carcinoma, and it is in body Mesostate methyl enol there is stronger active anticancer.Selenium is sought with Vitamin E, garlicin, linoleic acid, germanium, zinc etc. Foster element has collaborative antioxidative effect, increases antioxidant activity.Meanwhile, selenium has the work mitigating and alleviating heavy metal toxicity With.
But, the Ganoderma quantity of growth is few naturally, and containing substantial amounts of crude fibre and other impurity, and ganoderan contains Measure not high (usually 2-5%), be not easy to directly as health food or pharmaceutical raw material, therefore, development can improve ganoderan Studying with the artificial culture of the Ganoderma of Se content and cultivation technique is the key of cultivation of glossy ganoderma industrialization.
Content of the invention
It is an object of the invention to provide the Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved, can effectively improve Main active ganoderan in Ganoderma and the content of selenium.
The Lucid ganoderma pocket planting culture medium that ganoderan and Se content can be improved of the present invention, is former by following weight parts Material is made:75 parts of wood flour, 20 parts of Testa Tritici, 2 parts of Semen Maydis powder, 1 part of Calx, 1 part of Gypsum Fibrosum, 1 part of sugar, adjust material using Selenium-rich nutrient solution Water is than for 1:1.3-1.5;
Described Selenium-rich nutrient solution, is prepared by the following preparation method:
1. raw material prepares:Prepare 100 parts of pure water, 1.0 parts of L- Selenomethionine, 5 parts of Semen Maydis powder;
2. after raw material 1. mixes by step, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min, Then in 1-3s, quick release pressure obtains serosity, at 25 DEG C by the hay bud that percent by volume is that maturation is cultivated in 10% access Spore bacillus liquid spawn, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator 250r/ Min, fermentation time is 4h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 1.0h inactivation, obtain Arrive.
The cultural method of described bacillus subtilises liquid spawn is:Using triangular flask strain cultivation, below attach most importance to Amount percent, by the albumen processing slurry 30.0-35.0%, KH2P041.0%, MgS042.0%, NaCl 0.5% puts into In triangular flask, stir, adjust pH to 5.6,125 DEG C of sterilizing 10min, be cooled to 25 DEG C, access withered through slant culture Careless Bacillus, 28 DEG C of shaking table cultures 60-100h, for fermentation tank inoculation.
The preparation method of culture medium of the present invention, including spice, pack, sterilizing etc., with reference to the method for prior art.
Compared with prior art, the invention has the advantages that:
1st, Selenium-rich nutrient solution of the prior art, usually using inorganic selenium, particularly sodium selenite and sodium selenate, sub- selenium Sour potassium and potassium selenate etc., obtain from the side-product of metal mineral reserves, and inorganic selenium have larger toxicity, and are difficult to be absorbed, as Nutritional solution, assimilation effect is bad.The Selenomethionine that the present invention uses, is a kind of organic selenium source, compared with inorganic selenium, Selenomethionine There is safe and efficient advantage, it can substitute some methionine and be attached to one reversible selenium depots of formation on body protein, And there is not toxic hazard.
2nd, Selenomethionine is that in nature, selenium is present in one of plant and feed grain seleno amino with organic form The plant growings such as acid, frumentum and forage crop are not necessarily required to Selenomethionine, but with Methionine synthesis they can utilize ring Selenite particularly in soil and selenate synthesis Selenomethionine in border.Available selenium in soil is more, in plant Selenomethionine content is higher.At present, Selenomethionine is mainly derived from natural selenium yeast, is being primarily present of organic selenium in selenium yeast Form, but Selenomethionine has dense yeast taste, directly water-soluble rear sprinkling, and absorption efficiency is low and influences whether finally to produce The taste and flavor of product.Bacillus subtillis, are one kind of Bacilluss, and bacillus subtilises thalline itself synthesizes alphalise starch The enzymes such as enzyme, protease, Digestive Enzyme, cellulase, are equivalent to compound enzyme, and the present invention is by Selenomethionine mixing Ko subtilis bar Bacterium after fermentation, is fermented using the zymasises that Selenomethionine product carries, and is on the one hand beneficial to selenium element and enters Ganoderma, on the other hand With Methionine synthesis they can be using the selenite particularly in soil and selenate synthesis Selenomethionine in environment, also Can be effectively improved, dissolve yeast flavor.
3rd, steam explosion be using high-temperature high-pressure steam process biomass material, and realized by moment pressure leak process right The surface texture change of raw material, Component seperation and hemicellulose degradation, are generally used for corn straw, straw and Wooden Biomass etc. The pretreatment of raw material.The present invention passes through " to put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min, then in 1- Pressure is reduced to 0 by moment by quick release pressure in 3s ", on the one hand can change to sterilize by the moment of pressure, another Aspect can effectively refine, disperse Selenomethionine, advantageously in the absorption of Ganoderma.
Specific embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1-5:
In order to verify the technique effect of the present invention, 2016, produce the product of 5 batches using the technology of the present invention, each The method of batch is as follows:
1) preparation of culture medium:It is made up of the raw material of following weight parts:Wood flour 75kg, Testa Tritici 20kg, Semen Maydis powder 2kg, stone Grey 1kg, Gypsum Fibrosum 1kg, sugared 1kg, adjusting material-water ratio using Selenium-rich nutrient solution is 1:1.3-1.5;
2) spice, pack, sterilizing, inoculation;
3) send out bacterium:After inoculation, put into booth and send out bacterium, control greenhouse temperature at 26-28 DEG C;
4) go out sesame:After the long purseful of mycelia, Ganoderma enters out the sesame phase, controls greenhouse temperature to 26-28 DEG C;
Step 1) described in Selenium-rich nutrient solution, be prepared by the following preparation method:
1. raw material prepares:Prepare 100 parts of pure water, 1.0 parts of L- Selenomethionine, 5 parts of Semen Maydis powder;
2. after raw material 1. mixes by step, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min, Then in 1-3s, quick release pressure obtains serosity, at 25 DEG C by the hay bud that percent by volume is that maturation is cultivated in 10% access Spore bacillus liquid spawn, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator 250r/ Min, fermentation time is 4h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 1.0h inactivation, obtain Arrive.
Comparative example 1:
1) preparation of culture medium:It is made up of the raw material of following weight parts:Wood flour 75kg, Testa Tritici 20kg, Semen Maydis powder 2kg, stone Grey 1kg, Gypsum Fibrosum 1kg, sugared 1kg, adjusting material-water ratio using Selenium-rich nutrient solution is 1:1.3-1.5;
2) spice, pack, sterilizing, inoculation;
3) send out bacterium:After inoculation, put into booth and send out bacterium, control greenhouse temperature at 26-28 DEG C;
4) go out sesame:After the long purseful of mycelia, Ganoderma enters out the sesame phase, controls greenhouse temperature to 26-28 DEG C;
Step 1) described in Selenium-rich nutrient solution, be prepared by the following preparation method:
1. raw material prepares:Prepare 100 parts of pure water, 1.0 parts of sodium selenite, 5 parts of Semen Maydis powder;
2. after raw material 1. mixes by step, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min, Then in 1-3s, quick release pressure obtains serosity, at 25 DEG C by the hay bud that percent by volume is that maturation is cultivated in 10% access Spore bacillus liquid spawn, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator 250r/ Min, fermentation time is 4h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 1.0h inactivation, obtain Arrive.
Comparative example 2:
1) preparation of culture medium:It is made up of the raw material of following weight parts:Wood flour 75kg, Testa Tritici 20kg, Semen Maydis powder 2kg, stone Grey 1kg, Gypsum Fibrosum 1kg, sugared 1kg, material-water ratio is 1:1.3-1.5;
2) spice, pack, sterilizing, inoculation;
3) send out bacterium:After inoculation, put into booth and send out bacterium, control greenhouse temperature at 26-28 DEG C;
4) go out sesame:After the long purseful of mycelia, Ganoderma enters out the sesame phase, controls greenhouse temperature to 26-28 DEG C.
Result:
Each batch stochastic sampling, after testing, result see table:

Claims (2)

1. the Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved it is characterised in that raw material system by following weight parts Become:
75 parts of wood flour, 20 parts of Testa Tritici, 2 parts of Semen Maydis powder, 1 part of Calx, 1 part of Gypsum Fibrosum, 1 part of sugar, adjust material water using Selenium-rich nutrient solution Than for 1:1.3-1.5;
Described Selenium-rich nutrient solution, is prepared by the following preparation method:
1. raw material prepares:Prepare 100 parts of pure water, 1.0 parts of L- Selenomethionine, 5 parts of Semen Maydis powder;
2. after raw material 1. mixes by step, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min, then In 1-3s, quick release pressure obtains serosity, at 25 DEG C by the bacillus subtilis that percent by volume is that maturation is cultivated in 10% access Bacteria liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, and speed of agitator 250r/min sends out The ferment time is 4h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 1.0h inactivation, obtain.
2. the Lucid ganoderma pocket planting culture medium that ganoderan and Se content can be improved according to claim 1 it is characterised in that:Step Suddenly the cultural method of 2. described bacillus subtilises liquid spawn is:Using triangular flask strain cultivation, below for weight Percent, by the albumen processing slurry 30.0-35.0%, KH2P041.0%, MgS042.0%, NaCl0.5% put into triangle In bottle, stir, adjust pH to 5.6,125 DEG C of sterilizing 10min, be cooled to 25 DEG C, access the hay bud through slant culture Spore bacillus species, 28 DEG C of shaking table cultures 60-100h, for fermentation tank inoculation.
CN201610869594.9A 2016-09-30 2016-09-30 The Lucid ganoderma pocket planting culture medium of ganoderan and Se content can be improved Pending CN106478236A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107646531A (en) * 2017-09-30 2018-02-02 安徽利民生物科技股份有限公司 A kind of preparation method of Se-rich lucid ganoderma culture medium
CN108812067A (en) * 2018-07-10 2018-11-16 昆山市正兴食用菌有限公司 A kind of uniform quality and stable culture medium of edible fungus formula treatment process

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101643710A (en) * 2009-08-25 2010-02-10 山东汇丰源农业科技发展有限公司 Bacillus subtilis and application thereof
CN104892097A (en) * 2015-05-11 2015-09-09 苏州葛家坞生物科技有限公司 Liquid culture medium of selenium-rich edible fungus and preparation method for liquid culture medium
CN105272412A (en) * 2015-10-23 2016-01-27 广西锦翰环保科技有限公司 Organic-Se nutrient solution for crops as well as preparation method and application of organic-Se nutrient solution
CN105409596A (en) * 2015-12-21 2016-03-23 广东中源农业发展有限公司 Ganoderma lucidum substitute cultivation method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101643710A (en) * 2009-08-25 2010-02-10 山东汇丰源农业科技发展有限公司 Bacillus subtilis and application thereof
CN104892097A (en) * 2015-05-11 2015-09-09 苏州葛家坞生物科技有限公司 Liquid culture medium of selenium-rich edible fungus and preparation method for liquid culture medium
CN105272412A (en) * 2015-10-23 2016-01-27 广西锦翰环保科技有限公司 Organic-Se nutrient solution for crops as well as preparation method and application of organic-Se nutrient solution
CN105409596A (en) * 2015-12-21 2016-03-23 广东中源农业发展有限公司 Ganoderma lucidum substitute cultivation method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107646531A (en) * 2017-09-30 2018-02-02 安徽利民生物科技股份有限公司 A kind of preparation method of Se-rich lucid ganoderma culture medium
CN108812067A (en) * 2018-07-10 2018-11-16 昆山市正兴食用菌有限公司 A kind of uniform quality and stable culture medium of edible fungus formula treatment process

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