KR20020096575A - Process for production of mushroom mycelium highly enriched in trace minerals and product thereof - Google Patents
Process for production of mushroom mycelium highly enriched in trace minerals and product thereof Download PDFInfo
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- KR20020096575A KR20020096575A KR1020010035250A KR20010035250A KR20020096575A KR 20020096575 A KR20020096575 A KR 20020096575A KR 1020010035250 A KR1020010035250 A KR 1020010035250A KR 20010035250 A KR20010035250 A KR 20010035250A KR 20020096575 A KR20020096575 A KR 20020096575A
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- mycelium
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/51—Concentration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/328—Foods, ingredients or supplements having a functional effect on health having effect on glycaemic control and diabetes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/50—Concentrating, enriching or enhancing in functional factors
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
본 발명은 고함량의 미량무기물을 함유하는 버섯 균사체의 생산방법 및 그 산물에 관한 것이다. 더욱 상세하게는 미강과 밀가루 같은 곡물가루를 함유하는 액체배지에 크롬, 셀레늄, 게르마늄과 같은 미량무기물을 첨가하여 배지를 제조하고 버섯 균사체를 접종하여 배양하는 방법 및 그 산물에 관한 것이다.The present invention relates to a method for producing mushroom mycelium containing a high content of trace minerals and a product thereof. More specifically, the present invention relates to a method for producing a medium by incorporating microorganisms such as chromium, selenium, and germanium into a liquid medium containing grain flour such as rice bran and wheat flour, and inoculating mushroom mycelium, and a product thereof.
버섯은 단백질 함량이 많을 뿐만 아니라 인체에 유익한 다른 많은 생리활성 물질을 갖고 있다. 최근 들어 이들 중 크롬, 셀레늄, 게르마늄과 같은 미량무기물이 신진대사에 미치는 직·간접의 영향이 밝혀지면서, 크롬, 셀레늄, 게르마늄과 같은 미량무기물의 생리적 기능이 특히 강조되고 있는 것은 이러한 미량무기물의부족에 의해 질병이 발생될 수 있기 때문이다. 또한 화학비료를 많이 쓰는 오늘날에는 토양에서 미량무기물이 점점 없어지기 때문에 더욱 이들의 공급이 점점 더 요구되고 있다. 당뇨병은 미량무기물인 크롬, 아연부족에 의해 발병이 촉진되는데 이는 인슐린의 활동력을 높이고 작용여건을 갖추어 주기 위해서는 크롬과 아연이라는 미량무기물이 필요하기 때문이다. 특히 크롬은 혈당조정인자로서의 크롬(GTF)이라 하여 효모에서 발견되고 있다. 셀레늄의 부족은 암의 발생원이 될 수 있으며, 또한 노화를 촉진하고 동맥경화증을 일으키기도 한다. 게르마늄은 인삼과 산삼등의 약리활성을 갖는 식물에 함유되어 있음이 발견된 후 근래에 항암제 및 간염치료제, 면역증진을 위한 기능성 식품으로 이용 연구되고 있다. 이러한 크롬, 셀레늄, 게르마늄과 같은 미량무기물의 공급원으로 효모가 주로 이용되고 있으나 영지, 운지 등의 버섯류에도 미량무기물들이 많이 존재하고 있다. 따라서 항암성분뿐만 아니라 크롬, 셀레늄, 게르마늄과 같은 미량무기물의 공급원으로서 고부가가치를 갖는 버섯 균사체를 생산하여 건강식품, 기능성식품 및 의약품의 바이오 소재로 이용할 수 있을 것이다.Mushrooms are high in protein and have many other bioactive substances that are beneficial to the human body. Recently, the direct and indirect effects of trace minerals such as chromium, selenium, and germanium on metabolism have been revealed, and the physiological function of trace minerals such as chromium, selenium, and germanium is particularly emphasized. This is because the disease can be caused by. In addition, today's heavy chemical fertilizers are increasingly required because their trace minerals are increasingly removed from the soil. Diabetes is promoted by the lack of trace minerals, chromium and zinc, because it requires trace minerals such as chromium and zinc to increase the activity of insulin and to maintain its working conditions. In particular, chromium is found in yeast as chromium (GTF) as a glycemic regulator. Lack of selenium can be a source of cancer and also promote aging and cause atherosclerosis. Germanium is found in plants with pharmacological activity, such as ginseng and wild ginseng, and has recently been used as anti-cancer, hepatitis, and functional foods for immune enhancement. Yeast is mainly used as a source of trace minerals such as chromium, selenium, and germanium, but there are many trace minerals in mushrooms such as ganoderma lucidum and fingerlings. Therefore, as a source of trace minerals such as chromium, selenium and germanium as well as anticancer components, mushroom mycelium having high added value can be produced and used as bio material for health food, functional food and medicine.
본 발명자들은 상기와 같은 점을 착안하여 곡물가루를 함유하는 액체배지에 미량무기물을 첨가하여 제조된 배지에 버섯 균사를 접종하고 배양하여 저렴한 비용으로 고함량의 미량무기물을 함유하는 버섯 균사체를 생산함으로써 본 발명을 완성하였다.In view of the above, the present inventors inoculated and incubated mushroom mycelia on a medium prepared by adding a trace mineral to a liquid medium containing grain flour, thereby producing mushroom mycelium containing a high content of trace minerals at low cost. The present invention has been completed.
따라서 본 발명의 목적은 고함량의 미량무기물을 함유하는 버섯 균사체의 배양방법을 제공함에 있다.It is therefore an object of the present invention to provide a method for culturing mushroom mycelium containing a high content of trace minerals.
본 발명의 다른 목적은 상기 버섯 균사체 배양방법에 의해 얻어진 산물을 제공함에 있다.Another object of the present invention to provide a product obtained by the mushroom mycelium culture method.
본 발명의 상기 목적은 곡물가루를 함유하는 액체배지에 미량무기물을 첨가하여 멸균한 다음 상기 배지에 버섯 균사체를 접종한 후 20∼30℃의 배양실에서 고정배양인 경우에는 15∼30일간 배양하고 진탕배양인 경우에는 7∼15일간 배양한 다음 상기 배양된 균사체를 여과하여 회수한 후에 건조, 분말화하여 균사체 분말을 제조하고 열수농축하여 얻는 균사체열수농축액으로 음료를 제조함으로써 달성하였다.The object of the present invention is to sterilize by adding a microorganism to the liquid medium containing the powdered grains and inoculated mushroom mycelium in the medium and then cultured and shaken for 15 to 30 days in a fixed culture in a culture room at 20 ~ 30 ℃ In the case of cultivation, the cultured mycelium was cultured for 7 to 15 days and then collected by filtration, followed by drying and pulverizing to produce mycelium powder, and preparing a beverage with mycelium hydrothermal concentrate obtained by hydrothermal concentration.
이하, 본 발명의 구성을 설명한다.Hereinafter, the configuration of the present invention will be described.
도 1은 본 발명의 바람직한 실시예를 공정별로 도시한 개략도이다.1 is a schematic diagram showing a preferred embodiment of the present invention by process.
본 발명은 버섯 균사체의 종균을 아가 플레이트(agar plate) 또는 액체배지에 접종하여 배양하는 공정; 상기 버섯 균사체를 배양하기 위하여 미강과 밀가루를 각각 0.5% 첨가한 액체배지에 크롬, 셀레늄, 게르마늄과 같은 미량무기물을 첨가한 후 배양용기에 넣고 고압증기살균기나 밀페솥에서 20분간 살균하는 공정; 상기 제조한 배지를 냉각한 후 버섯 균사체의 종균을 접종하여 20∼30℃의 배양실에서 고정배양인 경우에는 15∼30일간 배양하고, 진탕배양인 경우에는 7∼15일간 배양하는 공정 ; 상기 균사체 배양액을 여과하여 균사체를 회수한 후에 건조, 분말화하여 균사체 분말을 제조하고 여액은 가열하여 농축액을 제조하는 공정 및 상기 제조한 버섯 균사체의 분말 및 버섯 균사 배양물을 이용하여 건강음료를 제조하는 공정으로 구성된다.The present invention is a step of inoculating the spawn of mushroom mycelium in an agar plate (agar plate) or a liquid medium; Adding microorganisms such as chromium, selenium, and germanium to a liquid medium to which 0.5% of rice bran and wheat flour are added to cultivate the mushroom mycelium, and then putting the same in a culture vessel and sterilizing for 20 minutes in a high pressure steam sterilizer or a wheat pepper pot; Cooling the prepared medium and inoculating the seedlings of the mushroom mycelium, culturing for 15 to 30 days in the case of fixed culture in a culture room at 20 to 30 ° C., and incubating for 7 to 15 days in the case of shaking culture; The mycelium culture solution was filtered to recover the mycelia, dried and powdered to prepare mycelium powder, and the filtrate was heated to prepare a concentrate, and a health beverage was prepared using the prepared mushroom mycelium powder and mushroom mycelia culture. It consists of a process to make.
상기 단계의 기능성 음료를 제조하는데 있어서, 크롬을 첨가한 액체배지를 사용하여 제조한 영지버섯 균사체 건조분말 1∼10g, 바람직하게는 3g과 셀레늄을 첨가한 액체배지를 사용하여 제조한 영지버섯 균사체 건조분말 0.01∼1g, 바람직하게는 0.15g을 미량 무기물이 첨가되지 않은 액체배지에서 배양하여 얻은 영지버섯균사 배양물 1L∼2L, 바람직하게는 1.5L에 혼합하여 농축한 다음 여과하여 당뇨병 치료에 뛰어난 효과가 있는 기능성 음료를 제조하거나, 상기 배양물 및 균사체 분말을 이용하여 음료에 통상 첨가되는 첨가물, 예를 들어 글루코스, 말토오즈, 사카로스, 올리고당, 천연꿀 등을 넣어서 통상의 음료제법에 따라 제품화 할 수 있다. 이때 미량무기물을 함유하는 버섯 균사체는 조성물 총 중량에 대하여 0.001∼1중량%로 함유되게 하는 것이 바람직하다.In preparing the functional beverage of the above step, Ganoderma lucidum mycelium dried powder prepared using a liquid medium containing chromium 1 ~ 10g, preferably ganoderma lucidum mycelium dried using a liquid medium containing 3g and selenium 0.01-1 g of powder, preferably 0.15 g, is mixed with 1 L to 2 L, preferably 1.5 L of Ganoderma lucidum mycelium culture obtained by culturing in a liquid medium to which no trace minerals are added, and then concentrated and filtered to excel in treating diabetes. Functional beverages can be prepared, or additives commonly added to beverages using the culture and mycelium powder, for example, glucose, maltose, saccharose, oligosaccharides, natural honey, etc., can be commercialized according to a conventional beverage preparation method. have. At this time, it is preferable that the mushroom mycelium containing the trace minerals is contained in 0.001 to 1% by weight based on the total weight of the composition.
상기 고함량의 미량무기물을 함유하는 버섯 균사체의 배양방법을 공정별로 상세히 설명한다.The culture method of the mushroom mycelium containing the high content of trace minerals will be described in detail for each process.
제 1공정: 버섯균사체의 종균 배양First step: spawn culture of mushroom mycelium
버섯균사체의 종균배양은 버섯균사체를 아가 플레이트(agar plate)상에서 배양하거나 액체배지에 접종하여 배양한다.The spawn culture of the mushroom mycelium is cultured by incubating the mushroom mycelium on an agar plate or inoculating it in a liquid medium.
제 2공정: 버섯 균사체 배양을 위한 배지 제조Second step: preparing a medium for culturing mushroom mycelium
물에 미강과 밀가루를 각각 0.5% 첨가한 배지에 크롬같은 미량무기물을 첨가한다. 상기의 혼합물을 배양용기에 넣고 잡균의 침입을 방지하기 위하여 뚜껑을 장착한 다음 이것을 고압증기살균기나 밀폐솥에서 20분간 살균한다.Add trace minerals such as chromium to the medium containing 0.5% of rice bran and flour in water. The mixture is placed in a culture vessel and a lid is attached to prevent invasion of various bacteria, and then sterilized for 20 minutes in a high pressure steam sterilizer or a closed pot.
상기 단계에서 크롬 외에도 셀레늄, 게르마늄같은 미량무기물을 사용할 수 있다.In addition to the chromium in this step, trace minerals such as selenium and germanium may be used.
상기 단계에서 버섯 균주에 따라 크롬(CrCl3)은 100∼1,200mg/L, 셀레늄(Na2SeO3)은 20∼300mg/L, 게르마늄(GeO2)은 200∼1,200mg/L을 첨가하여 사용할 수 있다. 바람직하게는 크롬, 셀레늄, 게르마늄을 각각 200mg/L, 50mg/L, 1,000mg/L을 첨가한다.Depending on the mushroom strain in this step, chromium (CrCl 3 ) is used in a 100 ~ 1,200mg / L, selenium (Na 2 SeO 3 ) 20 ~ 300mg / L, germanium (GeO 2 ) is added to use 200 ~ 1,200mg / L Can be. Preferably, 200 mg / L, 50 mg / L, 1,000 mg / L are added to chromium, selenium and germanium, respectively.
제 3공정: 버섯 균사체의 배양Third Step: Cultivation of Mushroom Mycelium
상기 제 2공정에서 제조한 멸균된 배지를 냉각한 다음 무균적으로 상기 제 1공정에서 배양한 버섯종균을 접종하여 20∼30℃의 배양실에서 고정배양인 경우에는 15∼30일간, 진탕배양인 경우에는 7∼15일간 배양한다.After cooling the sterilized medium prepared in the second step and aseptically inoculated mushroom seed cultured in the first step and in fixed culture in the culture room at 20-30 ℃ 15-15 days, if shaking culture Incubate for 7-15 days.
본 발명 고함량의 미량무기물을 함유하는 버섯 균사체의 배양배지에 접종할 수 있는 버섯 균사체는 영지, 운지, 치마, 느타리, 여름느타리, 표고, 팽이, 목이, 잎새, 싸리 및 맛버섯 등으로 식용 및 약용으로 사용되는 버섯이면 특별히 제한되지 않는다.Mushroom mycelium which can be inoculated in the culture medium of the mushroom mycelium containing a high content of trace minerals is edible and edible, such as ganoderma lucidum, fingering, skirt, zelkova, summer loin, shiitake, spinning top, sore throat, leafy bird, fever and taste mushroom, etc. If it is a mushroom used for medicinal purposes, it is not particularly limited.
제 4공정: 균사체 분말 및 배양농축액의 제조Fourth Step: Preparation of Mycelia Powder and Culture Concentrate
상기 제 3공정에서 배양된 배양물을 여과하여 균사체만을 회수한 다음 50∼60℃에서 12∼24 시간동안 건조한 뒤 분말화하여 균사체 분말을 제조하고 여액은 가열하여 농축액을 제조한다.The culture cultured in the third step is filtered to recover only the mycelium, and then dried at 50-60 ° C. for 12-24 hours to form a mycelium powder, and the filtrate is heated to prepare a concentrate.
상기 단계에서 제조한 균사체 분말은 기능성식품, 의약품 및 그 원료의 제조에 이용할 수 있고, 배양농축액은 사료에 첨가할 수 있다. 즉, 회수된 버섯 균사체에는 크롬, 셀레늄, 게르마늄과 같은 미량무기물의 생리활성물, 엑소폴리삭카라이드(exopolysaccharides)와 β-D-글루칸(β-D-glucan)등의 항암제, 균사배양액에는 엑소폴리삭카라이드(exopolysaccharides)와 β-D-글루칸(β-D-glucan)등의 항암제와 셀룰라아제(cellulases), 락카아제(laccases), 리파아제(lipase), 폴리갈락토우로나아제(polygalactouronases), 및 프로티아제(proteases)등의 수십종의 가수분해효소들 및 이러한 효소들에 의해 가수분해된 곡류의 여러 분해물질들이 존재할 뿐만 아니라 탈취제거와 같은 여러 가지 기능을 지닌 성분들을 포함하고 있기 때문에 이를 기능성식품, 의약품, 사료 및 그 원료의 제조에 이용할 수 있다.Mycelium powder prepared in the above step can be used for the production of functional foods, pharmaceuticals and raw materials, the culture concentrate can be added to the feed. That is, the recovered mushroom mycelium contains physiologically active substances of trace minerals such as chromium, selenium and germanium, anticancer agents such as exopolysaccharides and β-D-glucan, and exopolysaccharides for mycelial culture. Anticancer agents such as exopolysaccharides and β-D-glucan, cellulases, laccases, lipases, polygalacturonases, and pros Dozens of hydrolase enzymes such as proteases and various degradation products of grains hydrolyzed by these enzymes exist as well as functional foods because they contain various functions such as deodorization. It can be used to manufacture pharmaceuticals, feeds and raw materials.
이하, 본 발명의 구체적인 방법을 실시예를 들어 상세히 설명하고자 하지만,본 발명의 권리범위는 이들 실시예에만 한정되는 것은 아니다.Hereinafter, the specific method of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited only to these Examples.
실시예 1: 고함량의 크롬을 함유하는 버섯균사체 배양 및 그 산물 생산Example 1 Cultivation of Mushroom Mycelium Containing High Content of Chromium and Its Product Production
물 2L에 미강과 밀가루를 각각 0.5% 첨가한 배지에 크롬(CrCl3)을 200mg/L 첨가하여 액체배지를 제조한 다음 오토클래브에서 20분간 멸균하였다. 상기 멸균시킨 배지에 영지버섯균사의 종균을 무균적으로 접종하고 25℃에서 20일간 고정배양하였다. 배양 후 상기 액체배양액의 균사체를 여과하여 회수하고 열풍건조한 후 분말 6g을 얻었다. 상기 여액 1.8L는 100℃에서 1시간동안 가열농축하여 농축액 900mL를 제조하였다.200 mg / L of chromium (CrCl 3 ) was added to a medium in which 0.5% of rice bran and wheat flour were added to 2L of water to prepare a liquid medium, and then sterilized in an autoclave for 20 minutes. Sterile seedlings of the Ganoderma lucidum mycelium were sterilely inoculated into the sterilized medium and fixed cultured at 25 ° C. for 20 days. After incubation, the mycelia of the liquid culture solution were collected by filtration and dried by hot air to obtain 6 g of powder. 1.8L of the filtrate was concentrated by heating at 100 ° C. for 1 hour to prepare 900 mL of concentrate.
실시예 2: 고함량의 셀레늄을 함유하는 버섯균사체 배양 및 그 산물 생산Example 2: Cultivation of Mushroom Mycelium Containing High Content Selenium and Its Product Production
셀레늄(Na2SeO3) 50mg/L을 사용하여 상기 실시예 1과 동일하게 배지를 제조하고 영지버섯균사를 접종하여 배양한 다음 건조분말과 농축액을 제조하였다. 최종 제조된 건조분말은 6.5g이였으며 농축액은 880mL였다.Selenium (Na 2 SeO 3 ) using 50mg / L to prepare a medium in the same manner as in Example 1, inoculated with Ganoderma lucidum mycelium and incubated to prepare a dry powder and concentrate. The final dry powder was 6.5g and the concentrate was 880mL.
실시예 3: 고함량의 게르마늄을 함유하는 버섯균사체 배양 및 그 산물 생산Example 3: Cultivation of Mushroom Mycelium Containing High Content Germanium and Its Product Production
게르마늄(GeO2) 1,000mg/L을 사용하여 상기 실시예 1과 동일하게 배지를 제조하고 영지버섯균사를 접종한 다음 배양하여 건조분말과 농축액을 제조하였다. 최종 제조된 건조분말은 5.5g이였으며 농축액은 910mL였다.Using germanium (GeO 2 ) 1,000mg / L to prepare a medium in the same manner as in Example 1, inoculated Ganoderma lucidum mycelium and then cultured to prepare a dry powder and concentrate. The final dry powder was 5.5g and the concentrate was 910mL.
실시예 4: 본 발명 버섯균사체의 크롬함량 비교Example 4: Comparison of chromium content of the mushroom mycelium of the present invention
상기 실시예 1과 동일하게 배양하여 얻은 영지버섯균사체(실험군 1)와 상기실시예 1과 동일하나 크롬이 첨가되지 않은 배지에서 배양하여 얻은 영지버섯균사체(대조군)에 함유된 크롬함량을 비교하였다. 시험분석결과는 표 1에 나타난 바와 같이 실험군 1이 대조군보다 높았다.The chromium content contained in the Ganoderma lucidum mycelium obtained by culturing in the same manner as in Example 1 (Experimental Group 1) and the Ganoderma lucidum mycelium obtained in the same culture as in Example 1 but not added to chromium (control). As shown in Table 1, the test analysis result was higher in the experimental group 1 than the control group.
실시예 5: 본 발명 버섯균사체의 셀레늄함량 비교Example 5: Comparison of selenium content of the mushroom mycelium of the present invention
상기 실시예 2와 동일하게 배양하여 얻은 영지버섯균사체(실험군 2)와 상기 실시예 2와 동일하나 셀레늄이 첨가되지 않은 배지에서 배양하여 얻은 영지버섯균사체(대조군)에 함유된 셀레늄함량을 비교하였다. 시험분석결과는 표 2에 나타난 바와 같이 실험군 2가 대조군보다 높았다.The selenium content contained in the Ganoderma lucidum mycelium obtained by culturing in the same manner as in Example 2 (Experimental Group 2) and the Ganoderma lucidum mycelium obtained in cultivation in the same medium as Example 2 but without the addition of selenium (control). As shown in Table 2, the test analysis result showed that the experimental group 2 was higher than the control group.
실시예 6: 본 발명 버섯균사체의 열수농축액을 이용한 기능성음료의 제조Example 6: Preparation of functional beverage using hot water concentrate of the mushroom mycelium of the present invention
상기 실시예 1에서 얻은 영지버섯균사체 건조분말 3g(크롬함량: 약 2mg)과 상기 실시예 2에서 얻은 영지버섯균사체 건조분말 0.15g(셀레늄함량: 약 0.55mg)을 상기 실시예 1과 2와 동일하나 미량무기물(크롬,셀레늄)이 첨가되지 않은 배지에서배양하여 얻은 영지버섯균사배양물 1.5L에 혼합하여 감압농축기나 가열로 농축한 후 여과하여 약 1L의 균사체열수농축액을 얻었다.3g (chromium content: about 2mg) and Ganoderma lucidum mycelium dry powder obtained in Example 1 and 0.15g (selenium content: about 0.55mg) of Ganoderma lucidum mycelium dry powder obtained in Example 2 are the same as in Example 1 and 2 It was mixed with 1.5L of Ganoderma lucidum mycelium culture obtained by culturing in medium containing no trace minerals (chromium, selenium), concentrated by reduced pressure concentrator or heating, and filtered to obtain about 1L of mycelium hydrothermal concentrate.
상기에서와 같이 제조한 본 발명 음료를 당뇨병 환자 5명을 대상으로 맛에 대한 기호도와 당뇨치료효과를 조사하였다. 맛에 대한 기호도에 있어서 5명 모두 우수하다고 하였다. 당뇨치료효과는 본 발명 음료를 1일 1회 100mL씩 복용 후 3주일까지 혈당치를 측정한 결과, 1명이 35%, 1명이 20%, 2명이 14% 감소하여 복용 후 3주일만에 80%의 당뇨환자에서 효과를 보았다. 더욱이 35%의 혈당감소를 보여준 환자는 본 발명 음료의 복용 중 기존에 이용하던 인슐린의 양을 줄일 수 있었다. 효과를 보여주지 못한 1명은 평상시 기복이 심한 혈당치를 보여준 환자였다. 그러나 이 환자는 콜레스테롤양에 있어서는 23%의 감소를 보여주었다. 따라서 본 발명 음료를 3주일이상 복용한 환자들은 100% 모두 효과를 보았다. 본 발명 음료는 당뇨환자에 효과적인 기능성음료이면서 고콜레스테롤, 고지혈증, 비만증, 천식, 불면증 등을 가진 환자들도 효과를 볼수 있다.The beverages of the present invention prepared as described above were examined for palatability and diabetes treatment effects on five patients with diabetes. All five said it was excellent in taste level. Diabetes treatment effect was measured by blood glucose levels up to 3 weeks after taking 100 mL of the beverage of the present invention once a day, one person 35%, one person 20%, two people 14% decrease, 80% after 3 weeks The effect was seen in diabetic patients. Moreover, patients who showed a blood sugar reduction of 35% were able to reduce the amount of insulin previously used while taking the beverage of the present invention. One patient who did not show any effect was a patient with a severe ups and downs. However, the patient showed a 23% reduction in cholesterol. Therefore, the patients who took the beverage of the present invention for three weeks or more had 100% effect. The beverage of the present invention is an effective functional beverage for diabetics, patients with high cholesterol, hyperlipidemia, obesity, asthma, insomnia, etc. can also be effective.
이상, 상기 실시예를 통하여 설명한 바와 같이 본 발명 고함량의 미량무기물을 함유하는 버섯 균사체의 생산방법은 미강과 같은 곡물가루를 함유하는 액체배지에 크롬, 셀레늄, 게르마늄과 같은 미량무기물을 첨가한 다음 멸균한 후 버섯 균사체의 종균을 접종하여 배양함으로써 저렴한 비용으로 고함량의 미량무기물을 함유하는 버섯 균사체를 생산하는 효과가 있으며 상기 배양된 버섯 균사체를 이용하여제조되는 기능성음료는 음료로서 맛도 우수하고 당뇨병 등의 성인병 치료 및 예방에 효능을 나타낼 수 있는 뛰어난 효과가 있으므로 건강식품산업상 매우 유용한 발명인 것이다.As described above, the method for producing mushroom mycelium containing a high content of trace minerals as described above in the present invention is to add a trace mineral such as chromium, selenium and germanium to a liquid medium containing grain powder such as rice bran. After sterilizing and inoculating the seedlings of mushroom mycelium, it is effective to produce mushroom mycelium containing high content of trace minerals at low cost, and the functional beverage produced using the cultured mushroom mycelium has excellent taste as a beverage. It is a very useful invention in the health food industry because it has an excellent effect that can show efficacy in the treatment and prevention of adult diseases such as diabetes.
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| KR10-2001-0035250A KR100430784B1 (en) | 2001-06-21 | 2001-06-21 | Beverage for treating diabetes comprising mushroom mycelium highly enriched in trace minerals |
| JP2001309489A JP2002191354A (en) | 2000-10-06 | 2001-10-05 | Method for producing mushroom mycelium |
| US09/971,002 US6737065B2 (en) | 2000-10-06 | 2001-10-05 | Method for producing mushroom mycelia and uses thereof |
| CN01139392A CN1358413A (en) | 2000-10-06 | 2001-10-08 | Method for preparing mushroom mycelium and use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20010103110A (en) * | 2001-10-09 | 2001-11-23 | 영농조합법인 자연과생명 | Health Assistance Food using Mushroom Mycelium, and the Production Method |
| KR100440919B1 (en) * | 2001-08-18 | 2004-07-21 | (주)국전약품 | Manufacture method of baidiomycetal mycelium contain germanium |
| KR100634095B1 (en) * | 2003-12-17 | 2006-10-17 | (주)아모레퍼시픽 | Method for producing mycelium of Agaricus blazei Murrill containing organic selenium |
| WO2012165735A1 (en) * | 2011-05-27 | 2012-12-06 | (주)해뜰날 | Pharmaceutical composition containing mushroom mycelium rice extract for treating diabetes or obesity |
| CN111518842A (en) * | 2019-10-10 | 2020-08-11 | 江南大学 | Method for biosynthesizing nano-selenium by utilizing needle mushrooms |
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| KR100893967B1 (en) | 2005-02-16 | 2009-04-20 | 김승 | Process for cultivating Agrocybe cylindracea containing minerals and fertilizer containing the by-product thereof |
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| JPS5824504A (en) * | 1981-08-07 | 1983-02-14 | Yuko Hochido | Assimilating and absorbing method of germanium in plant |
| JPS61158776A (en) * | 1984-12-29 | 1986-07-18 | Furukawa Mining Co Ltd | Production of mold of chromium-containing microorganism |
| JP2652226B2 (en) * | 1988-12-05 | 1997-09-10 | 鶴見曹達株式会社 | How to grow mushrooms |
| JPH05252829A (en) * | 1992-03-13 | 1993-10-05 | Nisshoku Corp | Cultivation of mushroom containing germanium |
| JPH09248058A (en) * | 1996-03-18 | 1997-09-22 | Tsurumi Soda Co Ltd | Production of mushroom containing organic germanium |
| WO1998054948A1 (en) * | 1997-06-06 | 1998-12-10 | The Penn State Research Foundation | Selenium enrichment of mushrooms |
| KR100380945B1 (en) * | 2000-10-06 | 2003-04-26 | 송재만 | Process for mycelial culture using washing water of the grain and product thereof |
| KR100422314B1 (en) * | 2001-06-21 | 2004-03-12 | (주)카비오텍 | Process for feeding ducks using medicinal mushrooms and product thereof |
| KR100408011B1 (en) * | 2001-01-08 | 2003-12-01 | 정국훈 | Media composition containing selenium source for cultivating mushroom, method for preparing the same, and seleniumcontaining mushroom produced using the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100440919B1 (en) * | 2001-08-18 | 2004-07-21 | (주)국전약품 | Manufacture method of baidiomycetal mycelium contain germanium |
| KR20010103110A (en) * | 2001-10-09 | 2001-11-23 | 영농조합법인 자연과생명 | Health Assistance Food using Mushroom Mycelium, and the Production Method |
| KR100634095B1 (en) * | 2003-12-17 | 2006-10-17 | (주)아모레퍼시픽 | Method for producing mycelium of Agaricus blazei Murrill containing organic selenium |
| WO2012165735A1 (en) * | 2011-05-27 | 2012-12-06 | (주)해뜰날 | Pharmaceutical composition containing mushroom mycelium rice extract for treating diabetes or obesity |
| CN111518842A (en) * | 2019-10-10 | 2020-08-11 | 江南大学 | Method for biosynthesizing nano-selenium by utilizing needle mushrooms |
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