CN100375784C - Complex function type northern Chinese caterpillar fungus and its production method - Google Patents
Complex function type northern Chinese caterpillar fungus and its production method Download PDFInfo
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Abstract
The present invention relates to composite functional Chinese caterpillar fungus and a preparing method thereof, which belongs to the technical field of biological fermentation. The present invention has the technical scheme that Chinese medicinal materials having the physiological and pharmacological functions are added in a liquid strain culture medium of the Chinese caterpillar fungus having a conventional formula, so the Chinese caterpillar fungus which is rich in the components of organic zinc, organic germanium and triterpenoid is produced, and the content of original drug effect components of the Chinese caterpillar fungus is enhanced; therefore, the medicinal functions of the Chinese caterpillar fungus can be strengthened and increased, and the present invention provides an ideal natural raw material with higher quality for medicines and health foods.
Description
Technical field
The invention belongs to technical field of biological fermentation, be specifically related to a kind of complex function type northern Chinese caterpillar fungus and production method thereof.
Background technology
Cordyceps militaris (L.) Link. has another name called Cordyceps militaris (L.) Link., is both two kinds that more than 380 of Cordyceps are the most famous, most precious in planting, drug effect is the highest with wild cordyceps.Cordyceps militaris (L.) Link. rich in proteins, 17 seed amino acids, 21 kinds of trace elements, 9 kinds of VITAMIN also contain medicinal ingredientss such as cordycepic acid, cordycepin, Cordyceps polysaccharide, SOD enzyme, nucleic acid derivative.Modern medicine proof Cordyceps militaris (L.) Link. has the function of enhancing immunity, anticancer growth; Can the sexual function improving obstacle, renal failure, essential thrombocytopenia; Irregular pulse and atrioventricular block there is mitigation capability.
Though Cordyceps militaris (L.) Link. contains multiple nutrients and medicinal ingredients, essential zinc, the element Ge of human nutrition very lacks, and lacks triterpene compound more.Sesquioxide Ge-132 in the organic germanium is a kind of immunostimulant, tangible anti-tumor activity arranged and removes effects such as free radical, anti-mutation, and the low no bone marrow toxicity of toxicity.Zinc element is the important composition composition of many enzymes in the body, has functions such as promoting to grow, improve the sense of taste.Triterpene compound can promoting digestion the organ function, rise and be good for the stomach, lowering blood pressure, protect liver and beautifying function, also have clean blood decontamination, promote the cell regeneration effect.These three kinds of materials all have synergy with the medicinal function of Cordyceps militaris (L.) Link. itself.
In the production method of present domestic Cordyceps militaris (L.) Link., the nutraceutical matrix of employing is comparatively single, and still lacking with the Chinese medicinal materials is property of medicine matrix, as the production method of main additive.In " the artificial culture Cordyceps militaris (L.) Link. improves the method (patent No. CN02109304.0) of its content of physiologically active substance ", adopt additives such as adding casein, peptone, silkworm chrysalis juice, sorghum rice, Semen Maydis powder, only improved the content of itself inherent physiologically active substance of cordycepic acid, cordycepin, Cordyceps polysaccharide etc. in the Cordyceps militaris (L.) Link., in " method for artificially culturing selenium-rich Northern Chinese caterpillar fungus (patent No. CN93117344.2) ", adopt rich selenium material as additive, only improved the content of organoselenium in the Cordyceps militaris (L.) Link.; The composition of organic zinc in the Cordyceps militaris (L.) Link., organic germanium and triterpene compound is improved, and nutritive ingredient is still comprehensive inadequately.
Summary of the invention
In view of top described problem, the invention provides a kind of complex function type northern Chinese caterpillar fungus and production method thereof, to improve the effective component of organic zinc, organic germanium and triterpene compound in the Cordyceps militaris (L.) Link., strengthen and increase the medicinal function of Cordyceps militaris (L.) Link., for medical and protective foods provide more high-quality and ideal natural matter.
The present invention is except that original bioactive ingredients cordycepic acid, cordycepin and Cordyceps polysaccharide, also contain organic zinc, organic germanium and triterpene compound, wherein, the content of organic zinc is 78～230mg/kg, the content of organic germanium is 0.001～0.5mg/kg, and triterpene compound content is 0～0.5%.Concrete technical process is as follows:
The first step: in the liquid spawn culture medium of conventional formulation, add 0.1%～10.0% Ganoderma powder and 0.1%～10.0% edible Formica fusca powder;
Second step: aforesaid liquid spawn culture medium in 121 ℃ of sterilizations 20～30 minutes, is naturally cooled to room temperature, insert the Cordyceps militaris bacterial classification, under 21～24 ℃ of conditions, shaking table shaking culture 5～7 days, oscillation frequency 95～110 times/minute;
The 3rd step: insert in the grain matrix of the bacterium of having gone out again, cultivated 10～15 days in 16～19 ℃ of following lucifuges, treat that mycelia covers with the irradiate light that gives 100～2001x behind the culture substrate, need maintain the temperature between 16～22 ℃ this moment, humidity 80～90%, the dazzling ventilation of cultivating container, 45～60 days sporophore maturations.
The 4th step: pluck sporophore, take nature to dry in the shade or, water content is controlled at below 12%, can obtain complex function type northern Chinese caterpillar fungus in the method for oven dry below 40 ℃.
Wherein, the Ganoderma powder of being added in liquid spawn culture medium can be Ganoderma spore powder, and the edible Formica fusca powder can be ant extract.Additive can make an addition in the liquid spawn culture medium, also can make an addition in the substratum of Cordyceps militaris (L.) Link. submerged fermentation.
The present invention utilizes mycelium or sporophore can constantly decompose, absorb, transform and accumulate the biological nature of nutrient from nutraceutical matrix, to add the content that Chinese medicinal ingredients increases organic zinc in the Cordyceps militaris (L.) Link., organic germanium and triterpene compound, wherein adding Ganoderma powder or Ganoderma spore powder can increase the composition of organic germanium Ge-132 in the Cordyceps militaris (L.) Link., triterpene compound, adds the content that edible Formica fusca powder or ant extract can increase the content of organic zinc and further strengthen triterpene compound.
Organic zinc is described as mankind's " flower of life ", and organic zinc content is very abundant in the ant, wherein again with the Changbai Mountain ant for, its contained zinc is higher 10～20 times than any food of nature, also contains special defects compounds such as triterpene compound, ATP.Balancing yin and yang, bone and joint diseases, cardiovascular and cerebrovascular diseases, health immunological disease, nervous system disorders there is the assisting therapy function.And just contain organic germanium Ge-132, triterpene compound in the main effective constituent of glossy ganoderma.Therefore, we add these two kinds of materials in the liquid spawn culture medium as additive, nutritive ingredient that Cordyceps militaris (L.) Link. lacked and trace element have been replenished, strengthened its original special biological function simultaneously, also make it increase some medicinal function that does not originally possess, become complex function type northern Chinese caterpillar fungus.Show through preliminary study, in the sporophore of the resulting Cordyceps militaris (L.) Link. of the present invention, the content of not only original effective component such as cordycepin, Cordyceps polysaccharide and cordycepic acid is improved, and has also improved the content of trace element zinc, germanium, has increased triterpene compound composition (seeing Table 1) simultaneously.In addition, ant extract more helps the bio-transformation of Cordyceps militaris (L.) Link. because the active substance in the Formica fusca powder is extracted.
Table 1, ordinary method and the present invention are to the contrast of main component content influence in the Cordyceps sporophore
| Main component | Unit | Cordyceps sporophore 1 | Cordyceps sporophore 2 | Increased percentage |
| Cordycepin | mg/g | 2.26 | 4.42 | 96％ |
| Cordycepic acid | mg/g | 121 | 137 | 13％ |
| Cordyceps polysaccharide | mg/g | 92.4 | 143 | 55％ |
| Zinc | mg/kg | 78 | 155 | 99％ |
| Germanium | mg/kg | 0.001 | 0.1 | 10000％ |
| Triterpene compound | ％ | 0 | 0.01 | ———— |
Cordyceps sporophore 1: ordinary method obtains Cordyceps sporophore
Cordyceps sporophore 2: the present invention obtains Cordyceps sporophore
The interpolation period of the present invention also has remarkable advantages to the medicinal function that improves and increase Cordyceps militaris.The at present employing makes an addition to additive in the solid medium in the solid state cultivation stage more, this stage is adopted normal-pressure sterilization method or autoclaving usually, and the normal-pressure sterilization time was long, above 10 hours, autoclaving needs at 128 ℃, kept 1～1.5 hour, the temperature height, the time is also longer, destroy bigger to the organic composition in the additive, cause the protein denaturation in the substance, some amino acid, nucleic acid, carbohydrate, VITAMIN are destroyed, and miss fire.The additive interpolation period in process of production is the production of hybrid seeds stage among the present invention, be added in the seed liquid nutrient medium, sterilising conditions be 121 ℃ 25 minutes, required sterilization time is short, and sterilising temp is low, destroys less to the organic composition in the additive, reduced loss of nutritive components in the additive as much as possible, nutrition and effective component in the sporophore are increased to some extent, and the content of its original effective component also obtains improving, and effect is obvious.
Embodiment
Embodiment one: if produce a kind of complex function type northern Chinese caterpillar fungus, concrete technical process is as follows:
The first step: the Ganoderma powder of interpolation 0.1% and 0.1% Formica fusca powder in the liquid spawn culture medium of conventional formulation;
Second step: aforesaid liquid spawn culture medium in 121 ℃ of sterilizations 25 minutes, is naturally cooled to room temperature, insert the Cordyceps militaris bacterial classification, under 21～22 ℃ of conditions, shaking table shaking culture 5 days, oscillation frequency 95 times/minute;
The 3rd step: insert again in the grain matrix of the bacterium of having gone out, cultivated 10～12 days, treat that mycelia covers with the irradiate light that gives 100lx behind the culture substrate in 16～18 ℃ of lucifuges, need maintain the temperature between 16-18 ℃ this moment, humidity 80～84%, the dazzling ventilation of cultivating container, 45-60 days sporophore maturations.
The 4th step: pluck sporophore, in oven dry below 40 ℃, controlling moisture content is 10～12%, obtains complex function type northern Chinese caterpillar fungus.
Contain organic zinc 79mg/kg in the product that obtains after testing, organic germanium 0.001mg/kg triterpene compound 0.001%.
Embodiment two: if produce a kind of complex function type northern Chinese caterpillar fungus, concrete technical process is as follows:
The first step: the Ganoderma spore powder of interpolation 2.0% and 9.0% Formica fusca powder in the liquid spawn culture medium of conventional formulation;
Second step: aforesaid liquid spawn culture medium in 121 ℃ of sterilizations 25 minutes, is naturally cooled to room temperature, insert the Cordyceps militaris bacterial classification, under 23 ℃ of conditions, shaking table shaking culture 7 days, oscillation frequency 100 times/minute;
The 3rd step: insert in the grain matrix of the bacterium of having gone out again, cultivated 12～14 days in 18 ℃ of following lucifuges, treat that mycelia covers with the irradiate light that gives 100-200lx behind the culture substrate, need maintain the temperature between 18～20 ℃ this moment, humidity 85～87%, the dazzling ventilation of cultivating container, 45～60 days sporophore maturations.
The 4th step: pluck sporophore, in oven dry below 40 ℃, controlling moisture content is controlled to be 8～10%, obtains complex function type northern Chinese caterpillar fungus.
Contain organic zinc 218mg/kg in the product that obtains after testing, organic germanium 0.02mg/kg triterpene compound 0.02%.
Embodiment three: if produce a kind of complex function type northern Chinese caterpillar fungus, concrete technical process is as follows:
The first step: the Ganoderma powder of interpolation 10.0% and 3.5% ant extract in the liquid spawn culture medium of conventional formulation;
Second step: aforesaid liquid spawn culture medium in 121 ℃ of sterilizations 30 minutes, is naturally cooled to room temperature, insert the Cordyceps militaris bacterial classification, under 22～24 ℃ of conditions, shaking table shaking culture 6 days, oscillation frequency 110 times/minute;
The 3rd step: insert again in the grain matrix of the bacterium of having gone out, cultivated 13～15 days, treat that mycelia covers with the irradiate light that gives 200lx behind the culture substrate in 18～19 ℃ of following lucifuges, need maintain the temperature between 20～22 ℃ this moment, humidity 88%, the dazzling ventilation of cultivating container, 45～60 days sporophore maturations.
The 4th step: pluck sporophore,, water content is controlled at below 8%, obtain complex function type northern Chinese caterpillar fungus in oven dry below 40 ℃.
Contain organic zinc 113mg/kg in the product that obtains after testing, organic germanium 0.47mg/kg, triterpene compound 0.45%.
Embodiment four: if produce a kind of complex function type northern Chinese caterpillar fungus, concrete technical process is as follows:
The first step: the Ganoderma spore powder of interpolation 6.0% and 7.0% ant extract in the liquid spawn culture medium of conventional formulation;
Second step: aforesaid liquid spawn culture medium in 121 ℃ of sterilizations 28 minutes, is naturally cooled to room temperature, insert the Cordyceps militaris bacterial classification, under 22～23 ℃ of conditions, shaking table shaking culture 5～7 days, oscillation frequency 105 times/minute;
The 3rd step: insert again in the grain matrix of the bacterium of having gone out, cultivated 12 days, treat that mycelia covers with the irradiate light that gives 180lx behind the culture substrate in 16～18 ℃ of following lucifuges, need maintain the temperature between 18～20 ℃ this moment, humidity 90%, the dazzling ventilation of cultivating container, 45-60 days sporophore maturations.
The 4th step: pluck sporophore,, make water content be controlled at 12～15%, obtain complex function type northern Chinese caterpillar fungus in oven dry below 40 ℃.
Contain organic zinc 148mg/kg in its product that obtains after testing, organic germanium 0.35mg/kg triterpene compound 0.28%.
Embodiment five: if produce a kind of complex function type northern Chinese caterpillar fungus, concrete technical process is as follows:
The first step: the Ganoderma spore powder of interpolation 10.0% and 10.0% ant extract in the liquid spawn culture medium of conventional formulation;
Second step: aforesaid liquid spawn culture medium in 121 ℃ of sterilizations 25 minutes, is naturally cooled to room temperature, insert the Cordyceps militaris bacterial classification, under 22～23 ℃ of conditions, shaking table shaking culture 5～7 days, oscillation frequency 105 times/minute;
The 3rd step: insert again in the grain matrix of the bacterium of having gone out, cultivated 12 days, treat that mycelia covers with the irradiate light that gives 180lx behind the culture substrate in 16～18 ℃ of following lucifuges, need maintain the temperature between 18～20 ℃ this moment, humidity 90%, the dazzling ventilation of cultivating container, 45-60 days sporophore maturations.
The 4th step: pluck sporophore,, make water content be controlled at 10～12%, obtain complex function type northern Chinese caterpillar fungus in oven dry below 40 ℃.
Contain organic zinc 183mg/kg in its product that obtains after testing, organic germanium 0.33mg/kg, triterpene compound 0.25%.
Claims (3)
1. complex function type northern Chinese caterpillar fungus, except that original bioactive ingredients cordycepic acid, cordycepin and Cordyceps polysaccharide, also contain organic zinc, organic germanium and triterpene compound, it is characterized in that, the content of organic zinc is 78～230mg/kg, the content of organic germanium is 0.001～0.5mg/kg, and triterpene compound content is 0～0.5%, and its technological process of production is as follows:
The first step: in the liquid spawn culture medium of conventional formulation, add 0.1%～10.0% Ganoderma powder and 0.1%～10.0% edible Formica fusca powder;
Second step: aforesaid liquid spawn culture medium in 121 ℃ of sterilizations 20～30 minutes, is naturally cooled to room temperature, insert the Cordyceps militaris bacterial classification, under 21～24 ℃ of conditions, shaking table shaking culture 5～7 days, oscillation frequency 95～110 times/minute;
The 3rd step: insert in the grain matrix of the bacterium of having gone out again, cultivated 10～15 days in 16～19 ℃ of following lucifuges, treat that mycelia covers with the irradiate light that gives 100～200lx behind the culture substrate, need maintain the temperature between 16～22 ℃ this moment, humidity 80～90%, the dazzling ventilation of cultivating container, 45～60 days sporophore maturations;
The 4th step: pluck sporophore,, water content is controlled at below 15%, obtain complex function type northern Chinese caterpillar fungus in oven dry below 40 ℃.
2. a kind of complex function type northern Chinese caterpillar fungus according to claim 1 is characterized in that, adds 0.1%～10.0% Ganoderma spore powder and 0.1%～10.0% ant extract in the liquid spawn culture medium of conventional formulation.
3. the production method of a kind of complex function type northern Chinese caterpillar fungus according to claim 1 is characterized in that, additive Ganoderma powder, edible Formica fusca powder are added in the substratum of Cordyceps militaris (L.) Link. submerged fermentation.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TW200948371A (en) * | 2008-05-29 | 2009-12-01 | Jingwang Biotechnology Co Ltd | Cultivation method and liquid culture medium for cultivation of mycelium and fruiting body of Cordyceps |
| CN102523908A (en) * | 2010-12-08 | 2012-07-04 | 解竣文 | Cordyceps militaris strain liquid amplification technology |
| CN103005402B (en) * | 2012-12-31 | 2014-07-23 | 厦门金达威集团股份有限公司 | Method for producing cordyceps sinensis strain powder through liquid fermentation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100754A (en) * | 1993-09-24 | 1995-03-29 | 赵凤英 | Method for cultivating Chinese caterpillar fungus |
| CN1445360A (en) * | 2002-03-20 | 2003-10-01 | 中国科学院沈阳应用生态研究所 | Method of artificial planting northerly Chinese caterpillar fungus for increasing content of physiologically active substance |
| CN1546645A (en) * | 2003-12-15 | 2004-11-17 | 田敬华 | Northern Chinese caterpillar fungus culture liquid |
| CN1650686A (en) * | 2005-01-06 | 2005-08-10 | 江苏省农业科学院 | Solid culturing method of north cateripillar fungus |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100754A (en) * | 1993-09-24 | 1995-03-29 | 赵凤英 | Method for cultivating Chinese caterpillar fungus |
| CN1445360A (en) * | 2002-03-20 | 2003-10-01 | 中国科学院沈阳应用生态研究所 | Method of artificial planting northerly Chinese caterpillar fungus for increasing content of physiologically active substance |
| CN1546645A (en) * | 2003-12-15 | 2004-11-17 | 田敬华 | Northern Chinese caterpillar fungus culture liquid |
| CN1650686A (en) * | 2005-01-06 | 2005-08-10 | 江苏省农业科学院 | Solid culturing method of north cateripillar fungus |
Non-Patent Citations (1)
| Title |
|---|
| 富锌冬虫夏草菌丝体有机化程度及氨基酸含量分析. 贾乐，于克学，姚良同等.食品与发酵工业，第30卷第11期. 2004 * |
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