CN104054504A - Cultivation method of bee pupa cordyceps militaris - Google Patents

Cultivation method of bee pupa cordyceps militaris Download PDF

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CN104054504A
CN104054504A CN201310089236.2A CN201310089236A CN104054504A CN 104054504 A CN104054504 A CN 104054504A CN 201310089236 A CN201310089236 A CN 201310089236A CN 104054504 A CN104054504 A CN 104054504A
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bee pupa
bee
caterpillar fungus
chinese caterpillar
pupa
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CN104054504B (en
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陆九妹
冷云谷
冷雁
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Abstract

The invention discloses a cultivation method of bee pupa cordyceps militaris. According to the method, bee worms are taken as a material, a honeycomb spleen material is taken as a culture medium to ensure high quality and high content of functional components in a bee pupa cordyceps militaris product, a sterilized honeycomb is used to simulate a natural ecological environment of the bee pupa cordyceps militaris, and the bee pupa cordyceps militaris is artificially cultivated. The inoculation success rate and the muscardine rate after inoculation are high, and the bee pupa cordyceps militaris contains a large quantity of active ingredients of cordycepin, cordycepic acids, adenosine, polysaccharides, protein, SOD (superoxide dismutase) and the like, can boost immunity, prevent cancer and tumor, prevent aging, tonify the lung and the kidney and the like, and is a valuable and rare good product for dietary therapy and medicated food for supplementing nutrition and prolonging life.

Description

The breeding method of bee pupa Chinese caterpillar fungus
Technical field
The present invention relates to the breeding method of a kind of bee pupa Chinese caterpillar fungus.
Background technology
Natural cs is rare, expensive, adopt tame method, the Cordyceps militaris that utilizes mycelium to come submerged fermentation to turn out, drug effect is basic identical with Cordyceps sinensis, so being beneficial to silkworm chrysalis cultivation Cordyceps militaris family likes the bees rising in swarms, culture technique condition is uneven, occurs focusing on high yield in production, ignores the phenomenon of drug effect health care, industry price is striven fierceness unexpectedly, these bad behaviors have affected Chinese caterpillar fungus service quality, and price glides, and seriously restricts the sound development of China's Cordyceps militaris industry.
For break through suppressing the bottleneck of pupa worm industry development, we select the honeybee pupa worm with high-quality functional component is material, for improving the functional component of Cordyceps militaris, lays the foundation; Select on the other hand the cultivation matrix of honeycomb spleen material increase functional component, guaranteed high-quality and the high-load of functional component in bee pupa worm grass product.
Bee pupa has another name called honeybee embryo, honeybee is young.Being described as the treasure in food, is to contain abundant high protein, the desirable green nourishing food of low fat, a small amount of lecithin, multivitamin and trace element.Without any processing, be not polluted.Bee pupa both can be used as food, can be used as again nutrient and healthcare products.Bee pupa contains protein 20.3%, lipase 37 .5%, carbohydrate 19.5%, trace element 0.5%, moisture 42.7%.The nutritive value of bee pupa is not less than pollen; especially the content of vitamin A substantially exceeds beef; protein and dried fish wet goods are same; vitamin D surpasses 10 times of dried fish oil; containing the patron saint of human health " 21 century " germanium, selenium etc.; its contained active substance is that human body cannot produce voluntarily, is described as " ginseng in the sky ", " the refreshing worm of secret ".Have, brain tonic and intelligence development, strengthen the cytothesis of the organs such as memory, short liver kidney, nerve, body is upgraded, thus can benefiting qi and nourishing blood, the strong waist of strong kidney, declare lung ease constipation.
Honeycomb be honeybee store honey, slurry, powder, glue, wax, etc. material and the life area that raises up seed, the various countries expert discovery honeycomb of analyzing and researching contains the compositions such as beeswax, resin, grease, pigment, tannin, carbohydrate, organic acid, fatty acid and glucoside, a large amount of trace elements iron, calcium, copper, potassium, cobalt etc.Especially amino acid content is very abundant reaches 22.6%, and for 3.97 times of honey 5.7% and contain the very strong taurine of pharmacological action, average content reaches every gram of 0.154mg, is about every gram of honey containing (0.044mg) 3.5 times.In B.C. < < Sheng Nong's herbal classic > >, just have the note of " beeswax (honeycomb) taste is sweet, tepor, the nontoxic dense blood of lower disease, bowl spares, the beneficial gas of curing mainly " to plant, application honeycomb treatment rhinitis and blister sore, fatty liver also have very long history, modern study to show that honeycomb has the effects such as antibacterial, anti-inflammatory, inhibition tumour.China is bee-keeping big country, bee product aboundresources, honey, powder, slurry, glue, wax, the pupa of most of honeybee, all obtained good development utilization, only only have honeycomb only main as extracting the beeswax that wherein content is 25%, and other material that contains a large amount of nutrition and medicinal health does not well develop, cause the waste of resource and the pollution of environment.
Bee pupa Chinese caterpillar fungus contains a large amount of cordycepins, cordycepic acid, adenosine, worm polysaccharide, protein, SOD isoreactivity composition, can strengthen immunity, the anti-knurl that gives protection against cancer, anti-aging, invigorate the lung and the kidney etc., be the rare good merchantable brand of dietotherapy, herbal cuisine that human nutrition nourishing, the benefit of prolonging life lengthen one's life.
Summary of the invention
The present invention provides the breeding method of a kind of bee pupa Chinese caterpillar fungus in order to solve deficiency of the prior art, selecting the honeybee pupa worm with high-quality functional component is material, for improving the functional component of Cordyceps militaris, lays the foundation; Selected on the other hand honeycomb spleen material to increase cultivation matrix, the high-quality of guaranteeing functional component in bee pupa worm grass product and the content of functional component.
For achieving the above object, the technical solution used in the present invention is:
A breeding method for bee pupa Chinese caterpillar fungus, comprises the following steps
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25~45mL and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: live body bee pupa is carried out disinfection and makes host with thimerosal;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body, only inject by 0.15~0.25mg/;
(6) infect the protection of bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, stop up under temperature, protect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and grow stroma, method is with the loose nest slag after honeycomb Extraction parts nutriment, after sterilization treatment, on stiff pupal cell, cover one deck, want loose ventilative being placed at 15~30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 80~95% is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, Wa Chu Cheongju washes, and carries out body surface sterilization with edible alcohol, then be placed under 60 ℃ of environment and dry 2~4 hours, put shady and cool dry place sealing and preserve.
As a kind of preferred version of the breeding method of bee pupa Chinese caterpillar fungus of the present invention, described thimerosal is a kind of in 75% alcohol or amino acid iodine.
A kind of preferred version as the breeding method of bee pupa Chinese caterpillar fungus of the present invention, the cultivation of described stroma is for by stiff pupa head upwards or pupal cell is equidirectional keeps flat, inserting or put into treated honeycomb slag adds weed tree sawdust and mixes on the porous nest bedstead of doing, be placed at 15~30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 80~95% is cultivated.
A kind of preferred version as the breeding method of bee pupa Chinese caterpillar fungus of the present invention, comprises the following steps:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25~45mL and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: dry bee pupa is pulverized to the loose nest slag of making after bee pupa powder and honeycomb Extraction parts nutriment and be mixed and made into medium, the spore suspension of making in step (3) is seeded in medium, be placed at 15~30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 80~95% is cultivated;
(5) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, pick, be placed under 60 ℃ of environment and dry 2~4 hours, put shady and cool dry place sealing and preserve.
Beneficial effect:
It is material that the breeding method of bee pupa Chinese caterpillar fungus of the present invention is selected the honeybee pupa worm with high-quality functional component, greatly improves the functional component of Cordyceps militaris; Select the cultivation matrix of honeycomb spleen material increase functional component, guaranteed high-quality and the high-load of functional component in bee pupa worm grass product; The nutriment that in step (7), honeycomb extracts and the complementary basis slag charge of this high nutrition can further be developed reasonable application, carry out special process and can produce high-grade bee pupa cordyceps militaris liquor, senior drink, nourishing-paste, food etc.; Residue can be used as some industrial chains such as animal feed and vegetable manure again, turns waste into wealth, and for apiculture, accelerates to increase economic benefit, develops in a healthy way, and promotes agricultural production and income and makes contributions.
Embodiment
Embodiment 1
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25 and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: by the 75% alcohol 6-8 minute that carries out disinfection for live body bee pupa;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body, only inject by 0.15mg/;
(6) infect the protection of bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, stop up under temperature, protect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and grow stroma, method is by stiff pupa head upwards or pupal cell is equidirectional keeps flat, inserting or put into treated honeycomb slag adds weed tree sawdust and mixes on the porous nest bedstead of doing, be placed at 15~30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 80~95% is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, Wa Chu Cheongju washes, and carries out body surface sterilization with edible alcohol, then put under 60 ℃ of environment and dry 2 hours, put shady and cool dry place sealing and preserve.
Embodiment 2
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 35mL and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: by the 75% alcohol 6-8 minute that carries out disinfection for live body bee pupa;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body, only inject by 0.2mg/;
(6) infect the protection of bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, stop up under temperature, protect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and grow stroma, method is with the loose nest slag after honeycomb Extraction parts nutriment, after sterilization treatment, on stiff pupal cell, cover one deck, want loose ventilative being placed at 20 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 85% is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, Wa Chu Cheongju washes, and carries out body surface sterilization with edible alcohol, then put under 60 ℃ of environment and dry 2 hours, put shady and cool dry place sealing and preserve.
Embodiment 3
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 45mL and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: by amino acid iodine sterilization 1 minute for live body bee pupa;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body, only inject by 0.5mg/;
(6) infect the protection of bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, stop up under temperature, protect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and grow stroma, method is with the loose nest slag after honeycomb Extraction parts nutriment, after sterilization treatment, on stiff pupal cell, cover one deck, want loose ventilative being placed at 30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 95% is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, Wa Chu Cheongju washes, and carries out body surface sterilization with edible alcohol, then put under 60 ℃ of environment and dry 4 hours, put shady and cool dry place sealing and preserve.
Embodiment 4
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25~45mL and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: dry bee pupa is pulverized to the loose nest slag of making after bee pupa powder and honeycomb Extraction parts nutriment and be mixed culture bed, the spore suspension of making in step (3) is seeded in medium, be placed at 20 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 90% is cultivated;
(5) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, pick, be placed under 60 ℃ of environment and dry 2 hours, put shady and cool dry place sealing and preserve.
Embodiment 5
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25~45mL and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: dry bee pupa is pulverized to the loose nest slag of making after bee pupa powder and honeycomb Extraction parts nutriment and be mixed and made into medium, the spore suspension of making in step (3) is seeded in medium, be placed at 30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 90% is cultivated;
(5) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, pick, be placed under 60 ℃ of environment and dry 4 hours, put shady and cool dry place sealing and preserve.
Although embodiments of the present invention are illustrated in specification, these embodiments just, as prompting, should not limit protection scope of the present invention.Carrying out without departing from the spirit and scope of the present invention various omissions, displacement and change all should be included in protection scope of the present invention.

Claims (4)

1. a breeding method for bee pupa Chinese caterpillar fungus, is characterized in that, comprises the following steps
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25~45mL and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: live body bee pupa is carried out disinfection and makes host with thimerosal;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body, only inject by 0.15~0.25mg/;
(6) infect the protection of bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, stop up under temperature, protect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and grow stroma, method is with the loose nest slag after honeycomb Extraction parts nutriment, after sterilization treatment, on stiff pupal cell, cover one deck, want loose ventilative being placed at 15~30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 80~95% is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, Wa Chu Cheongju washes, and carries out body surface sterilization with edible alcohol, then be placed under 60 ℃ of environment and dry 2~4 hours, put shady and cool dry place sealing and preserve.
2. the breeding method of bee pupa Chinese caterpillar fungus according to claim 1, is characterized in that: the thimerosal in described step (4) is a kind of in 75% alcohol or amino acid iodine.
3. the breeding method of bee pupa Chinese caterpillar fungus according to claim 1, it is characterized in that: the cultivation of stroma is for by stiff pupa head upwards or pupal cell is equidirectional keeps flat in described step (7), inserting or put into treated honeycomb slag adds weed tree sawdust and mixes on the porous nest bedstead of doing, be placed at 15~30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 80~95% is cultivated.
4. the breeding method of a kind of bee pupa Chinese caterpillar fungus according to claim 1, comprises the following steps:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, organizes separated method to obtain the pure bacterial strain of Cordyceps sinensis the application of Cordyceps strain spore;
(2) the organic medium of bee pupa is made: the live body protein 10 0g that adopts live body bee pupa or peeling bee pupa worm, large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25~45mL and add thermal agitation miscible, complete after miscible and stop heating, the organic medium of bee pupa that packing test tube is jumped a queue is cooling, make semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated on the organic medium of bee pupa of (2) step making, put at 20 ℃ and to cultivate, after there is conidium, picking is partly placed on that in sterile water, to make spore suspension stand-by;
(4) host prepares: dry bee pupa is pulverized to the loose nest slag of making after bee pupa powder and honeycomb Extraction parts nutriment and be mixed and made into medium, the spore suspension of making in step (3) is seeded in medium, be placed at 15~30 ℃ of temperature, the natural lighting environmental condition that relative air humidity is 80~95% is cultivated;
(5) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, pick, be placed under 60 ℃ of environment and dry 2~4 hours, put shady and cool dry place sealing and preserve.
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CN105602787A (en) * 2016-02-05 2016-05-25 陆九妹 Bee pupa/Cordyceps militaris health-care wine, and preparation method and application thereof
CN111938022A (en) * 2020-08-31 2020-11-17 辽宁爱普罗斯饲料有限公司 Anti-bacterial feed additive adopting fungus solid state fermentation honeycomb residues and preparation and feeding method thereof
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