CN104770219A - Method for preparing cordyceps militaris - Google Patents
Method for preparing cordyceps militaris Download PDFInfo
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Abstract
The invention provides a method for preparing cordyceps militaris, comprising the following steps of preparing strains, preparing a cultivation substrate, cultivating a strain-receiving substrate, and harvesting and drying after the strains are cultivated maturely, wherein the step of preparing the strains is performed by means of cultivating cordyceps militaris strains in the substrate, cultivating by utilization of a rotary swing bed, and cultivating at a constant temperature for a certain time till mycelium pellets are formed and uniformly float; in the step of preparing the cultivation substrate, the cultivation substrate is composed of a substrate body and a culture solution; the ingredients of the culture solution comprise beans, coconut powder, pollen, glucose, magnesium sulfate, vitamin B1, vitamin B6 and the rest of alkalescent water; the material-to-liquid ratio of the substrate body to the culture solution is 1:1.7-1.9; the moisture content of a dried product is not greater than 8%. The cordyceps militaris prepared according to the method for preparing the cordyceps militaris has the advantages that the color is golden, conidial powder is contained, the contents of cordycepin, cordyceps polysaccharide and superoxide dismutase (SOD) are high, the formula and method provided by the invention are simple and are easily obtained, the ingredients which are used for preparing the culture solution are simple and are easily obtained, and the cost is low.
Description
Technical field
The invention belongs to field of food preparation, relate to cultivation and the production technology of a kind of Cordyceps militaris, particularly relate to the preparation method of a kind of Cordyceps militaris.
Background technology
Cordyceps militaris is Ascomycotina, Claviceps, Clavicipitaceae, the type sepecies of Cordyceps.Formal name used at school Cordycepsmilitaris, has another name called northern Chinese caterpillar Fungus, Cordceps militaris etc., and its natural quantity is little, and existing research is absorbed in and is cultivated domestication good quality strain, and batch production expands cultivates, and its dried product nutritive value is higher than wild.
The chemical analysis of Cordyceps militaris: N6-[β-(acetamide formyl) oxygen ethyl] adenosine; Chinese caterpillar fungus cyclic peptide A; Nucleosides; Ergosterol peroxide.The fine and soft Cordyceps militaris of Cordyceps militaris-pharmacological action modern science demonstration section not only has special nutritive value, and has obvious medical value.Possess four large Chinese caterpillar fungus compositions: cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide and Chinese caterpillar fungus SOD.
The medical value of the various bioactivators such as Cordyceps sinensis polysaccharide and SOD is the most remarkable.Cordycepic acid (mannitol) can fall low intracranial pressure significantly, promotes body metabolism, thus makes cerebral hemorrhage and cerebral thrombosis disease be eased.Cordycepin is a kind of nucleosides material with antibacterial activity, has very strong inhibitory action to core poly A polymerase.MRNA dysmaturity is made, the growth of inhibition cancer cell in DNA transcript mRNA process.And have hypoglycemic effect.Cordyceps sinensis polysaccharide is a kind of galactomannans of height branch, and it can promote lymphocyte transformation, improves the antibody content of serum IgG and the immunologic function of body, strengthens the anticancer ability pressing down cancer of body self.SOD(superoxide dismutase) superoxide radical in body can be eliminated, there is anti-ageing, the anticancer effect pressing down cancer.In addition; Cordyceps militaris is also containing abundant selenium (Se); this trace element by acknowledged be the trace element of needed by human; it is the activated centre of glutathione peroxidase; be connected on the peptide chain of zymoprotein with the form of selenocystein; the normal permeability of the stability of Cell protection film, and the generation of immune stimulatory globulin and antibody, strengthen immunity of organism and oxidation resistance.Meanwhile, a large amount of scientific practice proves that selenium can the growth of inhibition cancer cell significantly.
Cordycepin (cordycepin): antiviral, antibacterial, obvious Tumor suppression growth, interference human body RNA and DNA synthesis.
Cordycepic acid (PEARLITOL 25C): prophylactic treatment cerebral thrombus, cerebral hemorrhage, renal failure, diuresis.
Adenosine: antiviral, antibacterial, prophylactic treatment cerebral thrombus, cerebral hemorrhage, suppress Platelet congregation to prevent thrombosis, eliminate face spot, anti-ageing wrinkle resistant.
Cordyceps sinensis polysaccharide: improve immunity, delay senility, strengthen the body resistance to consolidate the constitution, cardioprotection, liver, anti-convulsion.
Ergosterol: anticancer, health care, attenuation.
Superoxide dismutase (SOD): suppress or eliminate to urge the superoxide radical of people's aging to be formed and anticancer, health care, attenuation.
Selenium (Se): the Anticancer Element Selenium of generally acknowledging for international medical community, is also important antioxidant, can strengthens body immunity.
For Cordyceps militaris, improve separately cordycepin content, Se content, zinc, iron, calcium three kinds of important element content in its fruit body and itself just there is certain technical difficulty; And expand numerous process very easily bacteria infection due to artificial culture Cordyceps militaris spawn, add external source antibiotic in the medium and become Technology development, and the antibiotic interpolation of this external source can cause very large human health potential safety hazard.Medical science finds: after antibiotic residual in human food enters human body, generally do not show as Acute Toxicity.But after the food of the residual antibiotic of long-term absorption low discharge, after certain hour, then may cause various chronic toxic effect due to residual antibiotic accumulation gradually in vivo.Common caused allergy (metamorphosis) reaction.Especially aminoglycoside medicaments, as streptomycin, gentamicin cause dysacousis.Its metabolism of penicillin medicine and degradation product have extremely strong sensitization.Penicillin has strong antigen, threatens maximum, and light causes dermatitis or itch, and serious causes the many aspects such as anaphylactic shock, toxic action, bacterial drug resistance, teratogenesis, mutagenesis and carcinogenesis, threat to life.Also can disturb the normal flora in people's enteron aisle.Therefore, cultivate Cordyceps militaris spawn and expand numerous stage, adopt and add foreign aid's antibiotic in the medium, though to control Cordyceps militaris spawn expand numerous process very easily bacteria infection problem have effect, but the Cordyceps militaris forming cultivation has penicillin and streptomycin to be remained, be easy to pollute human body and environment, cause the great potential safety hazard of produced from Cordyceps militaris L product
Summary of the invention
The object of the invention is by cultivating high-quality Cordyceps militaris original seed, be seeded in rice, coconut meat powder, cultivate in the nutritious synthetic medium that beans etc. make, and Artificial Control is carried out to its growing environment, to gather oven dry after the Cordyceps militaris maturation of cultivating, avoid the problem that artificial culture Cordyceps militaris spawn expands numerous process very easily bacteria infection, obtain the Cordyceps militaris dried product of nutrition active ingredient higher than wild Cordyceps sinensis.
Its technological process and process control condition as follows:
A preparation method for Cordyceps militaris, comprising: the preparation of bacterial classification, the preparation of culture medium for cultivating, and the medium after docking bacterium is cultivated, and carries out gathering and drying after cultivating maturation; Wherein, the preparation of described bacterial classification is by Cordyceps militaris spawn in the medium, adopts rotary shaker to cultivate, and cultivates certain hour to the mycelium pellet even suspension occurred under constant temperature; In the preparation of described medium, culture medium for cultivating is made up of medium and culture fluid, and the solid-liquid ratio of medium and culture fluid is 1:1.7-1.9; The oven dry goods moisture that described oven dry obtains is no more than 8%.
Concrete steps are as follows
One, material preparation technology:
(1) preparation of bacterial classification: preferably Cordyceps militaris spawn, cultivate at triangular flask liquid nutrient medium, the composition of liquid nutrient medium is potato, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, vitamin B1, vitamin B6, water; Obtained liquid nutrient medium is 0.11 MPa at pressure, sterilizing 25min under 122 DEG C of conditions; The preparation of bacterial classification adopts rotary shaker to cultivate, and the speed of rotation is 150 ~ 160r/min, 20 DEG C of constant temperature, and cultivating the mycelium pellet even suspension occurred in 4 ~ 6 days to triangular flask in dark can use.
(2) preparation of culture medium for cultivating: select rice medium, the nutriment composition of culture fluid is beans, coconut meat powder, pollen, glucose, magnesium sulfate, vitamin B1, vitamin B6, week-base water.In the manufacturing process of culture fluid, first soya bean or black soya bean are soaked by week-base water softening, break into soya-bean milk, afterwards dilute with water, and add coconut meat powder to it, pollen, glucose sugar, magnesium sulfate, vitamin B1, vitamin B6, and mix; The pH value of week-base water is 8 ~ 9; Mixed with rice medium by culture fluid afterwards, rice is 1:1.7 ~ 1.9 with the mixed liquor ratio of culture fluid, by full automatically subpackaging equipment, loaded and cultivated in cup, cover afterwards and cultivate lid, in pressure 0.11 MPa, temperature is that 122 DEG C of sterilizing 22min such as to cool at the bacterium waiting.
Two, connect bacterium to cultivate
It is connect bacterium to the media surface of cultivating in cup by after cultured liquid Cordyceps militaris spawn dilution that medium after docking bacterium carries out cultivating, by connect bacterium completely after cultivation cup move in culturing room and cultivate, cultivating early stage is light culture, temperature is 18 DEG C, humidity is 50% ~ 60%, by the time the fluorescent lamp light filling giving 8h ~ 10h when white hypha covers with media surface every day is cultivated, intensity of illumination average out to 130 lux; When media surface has the fruit body stroma of needle-like to occur, temperature is risen to 22 DEG C, relative air humidity is 70%; Cultivation cycle is 45 ~ 50 days, shoals can gather to fruit-body color.
Three, dry
Be layered on drying vehicle vehicle frame uniformly by the Cordyceps militaris of gathering before drying, drying initial temperature is 35 DEG C, and relative moisture is 34%, and this process dries 6h, and later stage bake out temperature is 48 DEG C, and relative moisture is 32%.Dry completely rear Cordyceps militaris water content control within 8%
Further, the formula of described liquid nutrient medium is by following mass percent preparation: potato 2.5% ~ 4%, magnesium sulfate 0.6 ~ 1.5%, potassium dihydrogen phosphate 1 ~ 1.2%, dipotassium hydrogen phosphate 0.2 ~ 0.5%, vitamin B1 0.008 ~ 0.015%, vitamin B6 0.005 ~ 0.015%, all the other are water.
Further, described beans is soya bean and/or black soya bean; Described pollen is cushaw pollen, Apple Pollen, the one in orange pollen or camellia pollen.
Further, the formula of described culture fluid is, calculates by mass percentage: beans 5 ~ 10%, coconut meat powder 5 ~ 10%, pollen 0.05 ~ 0.2%, glucose 5 ~ 8%, magnesium sulfate 0.5 ~ 1.0%, vitamin B1 0.003 ~ 0.012%, vitamin B6 0.005 ~ 0.015%, all the other are week-base water.
Preferably, the medium of described culture medium for cultivating and the solid-liquid ratio of culture fluid are 1:1.7, and the pH value of described week-base water is 8.5.
Beneficial effect of the present invention is:
1. culture medium for cultivating of the present invention comprises medium and culture fluid two parts composition, wherein culture fluid composition adopts beans, coconut meat powder etc. is rich in the material of vegetable protein as protein source, beans comprehensive nutrition, rich content, wherein the content of protein is higher than pork 2 times, is 2.5 times of egg content.The content of protein is not only high, and quality is good, is more easily absorbed by Cordyceps militaris; Beans contains the mineral salt such as abundant calcium, phosphorus, magnesium, potassium, also containing trace elements such as copper, iron, zinc, iodine, molybdenums; And coconut meat powder is rich in protein and carbohydrate and fat, simultaneously containing kind and all very abundant vitamin of content and mineral matter; Coconut meat albumen wherein is more easily absorbed by Cordyceps militaris, and its nutrition comparatively milk powder easily absorbs, and its rich content can provide sufficient nutrient supply, promotes Cordyceps militaris robust growth, thus improves its resistance to miscellaneous bacteria, suppresses living contaminants; The mineral ion that in coconut meat, content is the abundantest is magnesium and potassium, therefore can greatly reduce the usage amount of magnesium sulfate; Coconut meat is also rich in cocoa butter in addition, mineral matter, vitamin and polyphenols, the multiple necessary nutritive element of Cordyceps militaris can be supplied to and more easily be absorbed by Cordyceps militaris, it is except the nutrition of supply Cordyceps militaris, vitamin E wherein and polyphenols are excellent naturals, and it is antibacterial to have anti-inflammatory, improve the effect of the anti-miscellaneous bacteria ability of Chinese caterpillar fungus and insect protected; Pollen adopts cushaw pollen, Apple Pollen, one in orange pollen or camellia pollen, the composition such as several amino acids, vitamin A, vitamin C, vitamin E, active substance and trace elements of selenium, phosphatide, nucleic acid is rich in pollen, cushaw pollen wherein is especially rich in VB1, and camellia pollen amino acid content is high; Bean powder, coconut meat powder is in conjunction with the interpolation of pollen, and the active substance in functional component isoflavones wherein element, vitamin E and polyphenols and pollen can prevent and treat the problem that Cordyceps militaris spawn expands numerous process very easily bacteria infection.And composition is natural pollution-free in coconut meat powder and pollen, can not impact growing of Cordyceps militaris, prevent and treat artificial culture Cordyceps militaris spawn under solving Technology development and expand numerous bacteria infection, adopt and add external source antibiotic (penicillin and streptomycin) in the medium, cause the food-safety problem of very large human health potential safety hazard.Reach the pollution-free human body of Cordyceps militaris product of cultivation and the effect of environment hidden danger.
2, the Cordyceps militaris color that obtains of the present invention is golden yellow, and with conidial powder, cordycepin, Cordyceps sinensis polysaccharide, superoxide dismutase (SOD) content are high, and formula in the present invention and method simple and easy to get, the composition of preparation nutrient solution is simple and easy to get, with low cost.
Embodiment
Embodiment 1:
One, material preparation technology:
(1) bacterial classification preparation: preferably Cordyceps militaris spawn, cultivate at triangular flask liquid nutrient medium, its formula is potato 2.55%, magnesium sulfate 1.2%, potassium dihydrogen phosphate 1%, dipotassium hydrogen phosphate 0.45%, vitamin B1 0.008%, vitamin B6 0.014%, this formula is the PDA medium of improvement, be 0.11 MPa at pressure after making, sterilizing 25min under 122 DEG C of conditions.Be inoculated into by Cordyceps militaris original seed in triangular flask medium and cultivate, condition of culture is that shaking table is cultivated, and rotating speed is 160r/min, and temperature is 20 DEG C, and the mycelium pellet even suspension occurred in light culture about 5 days triangular flasks can use.
(2) medium preparing: select rice medium, the nutriment composition of culture fluid is soya bean 5% by mass percentage, coconut meat powder 9.5%, tea flower pollen 0.08%, glucose 7%, magnesium sulfate 0.55%, vitamin B1 0.005%, vitamin B6 0.0055%, all the other are week-base water.In the manufacturing process of culture fluid, first 5% soya bean is soaked by week-base water softening, break into soya-bean milk, afterwards dilute with water, and add coconut meat powder to it, tea flower pollen, glucose sugar, magnesium sulfate, vitamin B1, vitamin B6, and mix; The pH value of week-base water is 8.5; Mixed with rice medium by culture fluid afterwards, rice is 1:1.7 with the mixed liquor ratio of culture fluid, by full automatically subpackaging equipment, loaded and cultivated in cup, cover afterwards and cultivate lid, in pressure 0.11 MPa, temperature is that 122 DEG C of sterilizing 22min such as to cool at the bacterium waiting.Culture medium for cultivating comprises medium and culture fluid two parts composition, wherein culture fluid composition adopts beans, coconut meat powder etc. is rich in the material of vegetable protein as protein source, coconut meat is also rich in cocoa butter in addition, mineral matter, vitamin and polyphenols, can be supplied to the multiple necessary nutritive element of Cordyceps militaris and easily be absorbed by Cordyceps militaris, and it is except the nutrition of supply Cordyceps militaris, vitamin E wherein and polyphenols are excellent naturals, and have the antibacterial effect of anti-inflammatory; Pollen adopts cushaw pollen, Apple Pollen, the one in orange pollen or camellia pollen, be rich in pollen several amino acids, vitamin A, vitamin C,
vitamin E, the composition such as active substance and trace elements of selenium, phosphatide, nucleic acid, camellia pollen amino acid content is wherein high; Coconut meat powder is in conjunction with the interpolation of pollen, and the active substance in functional component vitamin E wherein and polyphenols and pollen can prevent and treat the problem that Cordyceps militaris spawn expands numerous process very easily bacteria infection.And composition is natural pollution-free in coconut meat powder and pollen, can not impact growing of Cordyceps militaris, prevent and treat artificial culture Cordyceps militaris spawn under solving Technology development and expand numerous bacteria infection, adopt and add external source antibiotic (penicillin and streptomycin) in the medium, cause the food-safety problem of very large human health potential safety hazard.Reach the pollution-free human body of Cordyceps militaris product of cultivation and the effect of environment hidden danger.
Two, connect bacterium to cultivate
Bacterium is connect to the media surface of cultivating in cup by after cultured liquid Cordyceps militaris spawn dilution, by connect bacterium completely after cultivation cup move in culturing room and cultivate, cultivating early stage is light culture, temperature is 18 DEG C, humidity is 50%, by the time the fluorescent lamp illumination of 8h is given when white hypha covers with media surface every day, intensity of illumination average out to 130 lux.By the time, when media surface has the fruit body stroma of needle-like to occur, temperature is risen to 22 DEG C of humidity and rise to 70%.General cultivation after 45 days fruit-body color shoal and just can gather.
Three, dry
Be layered on drying vehicle vehicle frame uniformly by the Cordyceps militaris of gathering before drying, drying initial temperature is 35 DEG C, and relative moisture is 34%, and this process dries 6h, and later stage bake out temperature is 48 DEG C, and relative moisture is 32%.Dry completely rear Cordyceps militaris water content control within 8%.
The Cordyceps militaris color that above-mentioned technique obtains is golden yellow, and with conidial powder, cordycepin content is 116mg/100g, and Cordyceps sinensis polysaccharide content is 19.07%, superoxide dismutase (SOD) 1145u/g.
Embodiment 2:
(1) bacterial classification preparation: preferably Cordyceps militaris spawn, cultivate at triangular flask liquid nutrient medium, its formula is potato 3.2%, magnesium sulfate 0.6%, potassium dihydrogen phosphate 1.15%, dipotassium hydrogen phosphate 0.3%, vitamin B1 0.010%, vitamin B6 0.010%, this formula is the PDA medium of improvement, be 0.11 MPa at pressure after making, sterilizing 25min under 122 DEG C of conditions.Be inoculated into by Cordyceps militaris original seed in triangular flask medium and cultivate, condition of culture is that shaking table is cultivated, and rotating speed is 155r/min, and temperature is 20 DEG C, and the mycelium pellet even suspension occurred in light culture 6 days triangular flasks can use.
(2) medium preparing: select rice medium, the nutriment composition of culture fluid is soya bean 7% by mass percentage, coconut meat powder 7.2%, cushaw pollen 0.15%, glucose 5.5%, magnesium sulfate 0.75%, vitamin B1 0.008%, vitamin B6 0.013%, all the other are week-base water.In the manufacturing process of culture fluid, first 7% black soya bean is soaked by week-base water softening, break into soya-bean milk, afterwards dilute with water, and add coconut meat powder to it, cushaw pollen, glucose sugar, magnesium sulfate, vitamin B1, vitamin B6, and mix; The pH value of week-base water is 8; Mixed with rice medium by culture fluid afterwards, rice is 1:1.8 with the mixed liquor ratio of culture fluid, by full automatically subpackaging equipment, loaded and cultivated in cup, cover afterwards and cultivate lid, in pressure 0.11 MPa, temperature is that 122 DEG C of sterilizing 22min such as to cool at the bacterium waiting.
Two, connect bacterium to cultivate
Bacterium is connect to the media surface of cultivating in cup by after cultured liquid Cordyceps militaris spawn dilution, by connect bacterium completely after cultivation cup move in culturing room and cultivate, cultivating early stage is light culture, temperature is 18 DEG C, humidity is 55%, by the time the fluorescent lamp illumination of 9h is given when white hypha covers with media surface every day, intensity of illumination average out to 130 lux.By the time, when media surface has the fruit body stroma of needle-like to occur, temperature is risen to 22 DEG C of humidity and rise to 70%.General cultivation after 50 days fruit-body color shoal and just can gather.
Three, dry
Be layered on drying vehicle vehicle frame uniformly by the Cordyceps militaris of gathering before drying, drying initial temperature is 35 DEG C, and relative moisture is 34%, and this process dries 6h, and later stage bake out temperature is 48 DEG C, and relative moisture is 32%.Dry completely rear Cordyceps militaris water content control within 8%.
The Cordyceps militaris color that above-mentioned technique obtains is golden yellow, and with conidial powder, cordycepin content is 115mg/100g, and Cordyceps sinensis polysaccharide content is 18.07%, superoxide dismutase (SOD) 1130u/g.
Embodiment 3:
(1) bacterial classification preparation: preferably Cordyceps militaris spawn, cultivate at triangular flask liquid nutrient medium, its formula is potato 4%, magnesium sulfate 1.0%, potassium dihydrogen phosphate 1.2%, dipotassium hydrogen phosphate 0.25%, vitamin B1 0.015%, vitamin B6 0.006%, this formula is the PDA medium of improvement, be 0.11 MPa at pressure after making, sterilizing 25min under 122 DEG C of conditions.Be inoculated into by Cordyceps militaris original seed in triangular flask medium and cultivate, condition of culture is that shaking table is cultivated, and rotating speed is 160r/min, and temperature is 20 DEG C, and the mycelium pellet even suspension occurred in light culture 5 days triangular flasks can use.
(2) medium preparing: select rice medium, the nutriment composition of culture fluid is the mixing 10% of soya bean and black soya bean by mass percentage, coconut meat powder 5.5%, orange pollen 0.10%, glucose 8%, magnesium sulfate 0.95%, vitamin B1 0.012%, vitamin B6 0.008%, all the other are week-base water.In the manufacturing process of culture fluid, first by black soya bean and soya bean in the mixing of 1:1 ratio, choose the black soya bean of 10% and the mixing beans week-base water of soya bean is soaked softening, break into soya-bean milk, dilute with water afterwards, and add coconut meat powder to it, orange pollen, glucose sugar, magnesium sulfate, vitamin B1, vitamin B6, and mix; The pH value of week-base water is 8.5; Mixed with rice medium by culture fluid afterwards, rice is 1:1.85 with the mixed liquor ratio of culture fluid, by full automatically subpackaging equipment, loaded and cultivated in cup, cover afterwards and cultivate lid, in pressure 0.11 MPa, temperature is that 122 DEG C of sterilizing 22min such as to cool at the bacterium waiting.
Two, connect bacterium to cultivate
Bacterium is connect to the media surface of cultivating in cup by after cultured liquid Cordyceps militaris spawn dilution, by connect bacterium completely after cultivation cup move in culturing room and cultivate, cultivating early stage is light culture, temperature is 18 DEG C, humidity is 60%, by the time the fluorescent lamp illumination of 10h is given when white hypha covers with media surface every day, intensity of illumination average out to 130 lux.By the time, when media surface has the fruit body stroma of needle-like to occur, temperature is risen to 22 DEG C of humidity and rise to 70%.General cultivation after 45 days fruit-body color shoal and just can gather.
Three, dry
Be layered on drying vehicle vehicle frame uniformly by the Cordyceps militaris of gathering before drying, drying initial temperature is 35 DEG C, and relative moisture is 34%, and this process dries 6h, and later stage bake out temperature is 48 DEG C, and relative moisture is 32%.Dry completely rear Cordyceps militaris water content control within 8%.
Obtained Cordyceps militaris color is golden yellow, and with conidial powder, cordycepin content is 118mg/100g, and Cordyceps sinensis polysaccharide content is 19.52%, superoxide dismutase (SOD) 1155u/g.
Embodiment 4:
(1) bacterial classification preparation: preferably Cordyceps militaris spawn, cultivate at triangular flask liquid nutrient medium, its formula is potato 3.5%, magnesium sulfate 1.5%, potassium dihydrogen phosphate 1.0%, dipotassium hydrogen phosphate 0.25%, vitamin B1 0.006%, vitamin B6 0.015%, this formula is the PDA medium of improvement, be 0.11 MPa at pressure after making, sterilizing 25min under 122 DEG C of conditions.Be inoculated into by Cordyceps militaris original seed in triangular flask medium and cultivate, condition of culture is that shaking table is cultivated, and rotating speed is 160r/min, and temperature is 20 DEG C, and the mycelium pellet even suspension occurred in light culture about 5 days triangular flasks can use.
(2) medium preparing: select rice medium, the nutriment composition of culture fluid is the mixing 6% of soya bean and black soya bean by mass percentage, coconut meat powder 8.5%, cushaw pollen 0.10%, glucose 6%, magnesium sulfate 0.85%, vitamin B1 0.075%, vitamin B6 0.014%, all the other are week-base water.In the manufacturing process of culture fluid, first by black soya bean and soya bean in the mixing of 1:1 ratio, choose the black soya bean of 6% and the mixing beans week-base water of soya bean is soaked softening, break into soya-bean milk, dilute with water afterwards, and add coconut meat powder to it, orange pollen, glucose sugar, magnesium sulfate, vitamin B1, vitamin B6, and mix; The pH value of week-base water is 8.5; Mixed with rice medium by culture fluid afterwards, rice is 1:1.7 with the mixed liquor ratio of culture fluid, by full automatically subpackaging equipment, loaded and cultivated in cup, cover afterwards and cultivate lid, in pressure 0.11 MPa, temperature is that 122 DEG C of sterilizing 22min such as to cool at the bacterium waiting.
Two, connect bacterium to cultivate
Bacterium is connect to the media surface of cultivating in cup by after cultured liquid Cordyceps militaris spawn dilution, by connect bacterium completely after cultivation cup move in culturing room and cultivate, cultivating early stage is light culture, temperature is 18 DEG C, humidity is 60%, by the time the fluorescent lamp illumination of 10h is given when white hypha covers with media surface every day, intensity of illumination average out to 130 lux.By the time, when media surface has the fruit body stroma of needle-like to occur, temperature is risen to 22 DEG C of humidity and rise to 70%.General cultivation after 45 days fruit-body color shoal and just can gather.
Three, dry
Be layered on drying vehicle vehicle frame uniformly by the Cordyceps militaris of gathering before drying, drying initial temperature is 35 DEG C, and relative moisture is 34%, and this process dries 6h, and later stage bake out temperature is 48 DEG C, and relative moisture is 32%.Dry completely rear Cordyceps militaris water content control within 8%.
Obtained Cordyceps militaris color is golden yellow, and with conidial powder, cordycepin content is 121mg/100g, and Cordyceps sinensis polysaccharide content is 20.32%, superoxide dismutase (SOD) 1158u/g.
Although be illustrated embodiments of the present invention in specification, these embodiments just as prompting, should not limit protection scope of the present invention.Carry out various omission, displacement and change without departing from the spirit and scope of the present invention all should be included in protection scope of the present invention.
Claims (10)
1. a preparation method for Cordyceps militaris, comprising: the preparation of bacterial classification, the preparation of culture medium for cultivating, and the medium after docking bacterium is cultivated, and carries out gathering and drying after cultivating maturation; It is characterized in that:
The preparation of described bacterial classification is by Cordyceps militaris spawn in the medium, adopts rotary shaker to cultivate, and cultivates certain hour to the mycelium pellet even suspension occurred under constant temperature;
In the preparation of described medium, culture medium for cultivating is made up of medium and culture fluid, and the composition of culture fluid comprises: beans, coconut meat powder, pollen, glucose, magnesium sulfate, vitamin B1, vitamin B6, and all the other are week-base water; The solid-liquid ratio of medium and culture fluid is 1:1.7-1.9;
The oven dry goods moisture that described oven dry obtains is no more than 8%.
2. according to the preparation method of the Cordyceps militaris described in claim 1, it is characterized in that: the preparation of described bacterial classification comprises: select Cordyceps militaris spawn, cultivate at triangular flask liquid nutrient medium, the composition of liquid nutrient medium is potato, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, vitamin B1, vitamin B6, water; Obtained liquid nutrient medium is 0.11 MPa at pressure, sterilizing 25min under 122 DEG C of conditions; The preparation of bacterial classification adopts rotary shaker to cultivate, and the speed of rotation is 150 ~ 160r/min, 20 DEG C of constant temperature, and cultivating the mycelium pellet even suspension occurred in 4 ~ 6 days to triangular flask in dark can use.
3. the preparation method of Cordyceps militaris according to claim 2, it is characterized in that: the formula of described liquid nutrient medium is prepared by following mass percent: potato 2.5% ~ 4%, magnesium sulfate 0.6 ~ 1.5%, potassium dihydrogen phosphate 1 ~ 1.2%, dipotassium hydrogen phosphate 0.2 ~ 0.5%, vitamin B1 0.008 ~ 0.015%, vitamin B6 0.005 ~ 0.015%, all the other are water.
4. according to the preparation method of the Cordyceps militaris described in claim 1, it is characterized in that: the culture medium for cultivating of described Cordyceps militaris is rice medium, the week-base water pH value in culture fluid is 8 ~ 9.
5. the preparation method of Cordyceps militaris according to claim 1, is characterized in that: described beans is soya bean and/or black soya bean; Described pollen is cushaw pollen, Apple Pollen, the one in orange pollen or camellia pollen.
6. the preparation method of Cordyceps militaris according to claim 1, it is characterized in that: the formula of described culture fluid is, calculate by mass percentage: beans 5 ~ 10%, coconut meat powder 5 ~ 10%, pollen 0.05 ~ 0.2%, glucose 5 ~ 8%, magnesium sulfate 0.5 ~ 1.0%, vitamin B1 0.003 ~ 0.012%, vitamin B6 0.005 ~ 0.015%, all the other are week-base water.
7. the preparation method of the Cordyceps militaris according to claim 4 or 5 or 6, it is characterized in that: in the manufacturing process of described culture fluid, first soya bean or black soya bean are soaked by week-base water softening, break into soya-bean milk, dilute by week-base water afterwards, and add coconut meat powder to it, pollen, glucose sugar, magnesium sulfate, vitamin B1, vitamin B6, and mix; Mixed with rice medium by culture fluid afterwards, rice is 1:1.7 ~ 1.9 with the mixed liquor ratio of culture fluid, by full automatically subpackaging equipment, loaded and cultivated in cup, cover afterwards and cultivate lid, in pressure 0.11 MPa, temperature is that 122 DEG C of sterilizing 22min such as to cool at the bacterium waiting.
8. according to the preparation method of the Cordyceps militaris described in claim 1, it is characterized in that: it is connect bacterium to the media surface of cultivating in cup by after cultured liquid Cordyceps militaris spawn dilution that the medium after described docking bacterium carries out cultivating, by connect bacterium completely after cultivation cup move in culturing room and cultivate, cultivating early stage is light culture, temperature is 18 DEG C, humidity is 50% ~ 60%, by the time the fluorescent lamp light filling giving 8h ~ 10h when white hypha covers with media surface every day is cultivated, intensity of illumination average out to 130 lux; When media surface has the fruit body stroma of needle-like to occur, temperature is risen to 22 DEG C, relative air humidity is 70%; Cultivation cycle is 45 ~ 50 days, shoals can gather to fruit-body color.
9. according to the preparation method of the Cordyceps militaris described in claim 1, it is characterized in that: be layered on drying vehicle vehicle frame uniformly by the Cordyceps militaris of gathering before described oven dry, drying initial temperature is 35 DEG C, and relative moisture is 34%, dries 6h; Later stage bake out temperature is 48 DEG C, and relative moisture is 32%, dries completely rear Cordyceps militaris water content control within 8%.
10. the preparation method of Cordyceps militaris according to claim 1, is characterized in that: the medium of described culture medium for cultivating and the solid-liquid ratio of culture fluid are 1:1.7, and the pH value of described week-base water is 8.5.
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| CN106386171A (en) * | 2016-08-31 | 2017-02-15 | 张玉军 | Method for producing Cordyceps militaris |
| CN107333568A (en) * | 2017-09-19 | 2017-11-10 | 张家港市藏联生物研究所有限公司 | A kind of more batches of culture techniques of Cordyceps militaris constant temperature |
| CN107926482A (en) * | 2017-12-08 | 2018-04-20 | 江西师范大学 | A kind of method using fluid nutrient medium production fruiting bodies of cordyceps militaris |
| CN109234176A (en) * | 2018-11-28 | 2019-01-18 | 湖南金芙农业科技有限公司 | Cordyceps sinensis mother culture media and preparation method thereof |
| CN112352945A (en) * | 2020-09-14 | 2021-02-12 | 上海国宝企业发展中心 | Artificial cordyceps militaris food with effects of resisting fatigue and improving immunity |
| CN114431070A (en) * | 2021-12-31 | 2022-05-06 | 重庆大学 | Cordyceps militaris large-scale production method and application |
| CN115491313A (en) * | 2022-10-09 | 2022-12-20 | 辽宁省农业科学院 | Cordyceps militaris white strain and application thereof |
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| CN114431070A (en) * | 2021-12-31 | 2022-05-06 | 重庆大学 | Cordyceps militaris large-scale production method and application |
| CN115491313A (en) * | 2022-10-09 | 2022-12-20 | 辽宁省农业科学院 | Cordyceps militaris white strain and application thereof |
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