KR20080067034A - Process for cultivating crops containing phyto-selenium with mixed mushrooms - Google Patents

Abstract

A method for cultivating crops containing organic selenium by using mixed mushroom germs is provided to obtain higher value-added crops containing a high concentration of organic selenium by inoculating the mixed mushroom germs into grains mixed with an inorganic selenium solution. A method for cultivating crops containing organic selenium by using mixed mushroom germs comprises the steps of: (a) adding malt to refined water, saccharifying at 55~66°C for 1day, boiling at 95~110°C for 20~30min, filtering, adjusting the sugar content of the malt extract to 10~13 brix, and sterilizing to make a malt extract-liquid culture medium; (b) pure-culturing Phellinus linteus germs, Inonotus Obliquus germs and king oyster mushroom germs in the liquid culture medium at 15~37°C for 7~14days to obtain each liquid spawn; (c) putting washed grains in a culture container, and sterilizing; (d) filter-sterilizing an inorganic selenium solution by a filter(0.15~0.3mum); (e) adding 80~120ppm of the filter-sterilized inorganic selenium solution to the sterilized grains to make a mixed culture medium; (f) inoculating 10~15% of each liquid spawn into the mixed culture medium; (g) culturing the inoculated spawns at 25~28°C for 20~30days; (h) adding 100ppm of an inorganic selenium solution to the mixed culture medium 2 times in the step (g); (i) shaking the culture container once every 2~3days when hyphae begin to grow on the whole surface of the grains, thereby preventing the grains from clumping together; and (j) lyophilizing or hot-air drying at 65~85°C, and then selecting.

Description

Process for cultivating crops containing phyto-selenium with mixed mushrooms

The present invention relates to a method of cultivating organic selenium-containing crops, and more particularly, by inoculating complex mushroom bacteria to a crop containing inorganic selenium solution, selenium has a pharmacological function through metabolism of mushrooms during mushroom growth. A method of converting to organic selenium form to allow organic selenium to be contained in crops.

Mushroom has been used for edible and medicinal for about 3,000 years, and it is reported that the mushroom-derived materials are useful for the treatment or prevention of various cancers and for the prevention of cancer caused by immune enhancement. Mushrooms are divided into Mycelium and Fruiting Body. Mycelium means that spores of mushrooms germinate to form a thread. Abundance of bioactive substances and germs are germinated to form sprouts It is called a mushroom.

As the immune system of the human body is activated, it is extremely difficult to develop a disease, and thus the medical community has recently been actively researching the human immune system. When cancer develops, the "human immune mechanism" acts to prevent its growth or metastasis. As an immune mechanism, researches on mushroom polysaccharides are being actively conducted, and among them, the results of the studies that the polysaccharides contained in mushrooms are very effective in treating cancer and are excellent in effect. Pharmacological studies of mushrooms have been known to activate immune function following chemotherapy following liver cancer, including gastric cancer, esophageal cancer, duodenal cancer, colon cancer, and rectal cancer, which are cancers of the digestive system. .

On the other hand, selenium is present in trace amounts in seawater and soil and is an essential mineral element in human cells. First discovered by Berzelius in 1817 and named selenium after Selen, the Greek mythological moon goddess, in 1957 it was spotlighted by Dr. Klaus Schwarz as a mineral essential for the activation of human cell function. Started. Phospholipids, which occupy most of the cell membranes, contain a large amount of unsaturated fatty acids. Lipid peroxides are those that are automatically oxidized by free radicals. In other words, when the cell membrane is chemically damaged by free radicals, the ability to defend the cell itself is lost, and the DNA, a genetic factor, is damaged. If the phospholipids in the cell membrane are attacked by free radicals and become lipid peroxides, and thus the cell membranes are not protected, the cells lose their natural function and proliferate abnormally and are converted to cancer cells. Healthy people neutralize themselves before free radicals damage our bodies, but when excessive free radicals occur at one time or when the body's defense capacity decreases, free radicals destroy the cells of our bodies in turn to promote aging of the human body. Attacks genes and causes mutations to cause cancer The body's defense enzyme that prevents the oxidation or destruction of cells by free radicals is glutathion-peroxidase, which is synthesized in the liver, and selenium is essential as a major component. In other words, the deficiency of selenium in the living body has a significant effect on the function of the human body, there is a risk of various adult diseases such as heart disease, joint disease, vision disease, aging of cells, deterioration of human immune function and aging and onset. The recommended daily dose of selenium is 75-200 μg. Selenium has been shown to prevent cancer, prevent metastasis, help prevent angina and myocardial infarction, and have anti-aging effects. Selenium is a mineral that is necessary to make `` prostaglandin '', a hormone that regulates blood pressure in the body, and prevents blood vessels from expanding or hardening, thereby preventing atherosclerosis, thrombosis, myocardial infarction, and cerebral thrombosis. In addition, it has a role as an antioxidant to remove the fat peroxide that causes aging. In other words, if a large amount of lipid peroxide is produced in the body, it may cause adult diseases such as arteriosclerosis, liver failure, diabetes, and cataract, but selenium is a component of an enzyme called glutathione-peroxidase, which breaks down the lipid peroxide. And play an important role in the activity of this enzyme. Therefore, selenium has the effect of anti-aging.

Among the functional crops that are currently on the market, for example, rice has been shown to include nutrients such as chitosan, minerals and calcium, as well as natural substances such as gungjungjang rice, ginseng rice, mushroom rice and green tea rice. New functional rice that does not need to be washed is also emerging. In addition, other bio-rice (bioactive rice), red mushroom rice, live rice (germ) are on the market.

Therefore, there is an urgent need for the development of a system for providing agricultural products in a safe manner without adverse effects on the human body by allowing minerals to be absorbed into plants in a more efficient manner.

As a result of earnestly researching the formulation of selenium which can be directly absorbed by consumers, the present inventors have added selenium to grains or crops such as brown rice, brown rice, black rice, and cereals, which are commonly used in diet, and cultivated complex mushroom bacteria using them as a medium. As a result, it is possible to prevent various diseases in advance by ingesting a crop of mushrooms, which contain various efficacies such as preventing various adult diseases, and crops containing organic selenium and nutrients, which exhibit various functions as described above, through daily meals. Unexpectedly, it was possible to complete the present invention.

Therefore, an object of the present invention is a composite crop containing organic selenium obtained by culturing mushroom complexes (eg chaga, matsutake and situation mushrooms) on crops (eg rice) to which selenium is added, and the organic selenium to the crops. It is to provide a method for complex cultivation of crops to be contained.

Another object of the present invention is to provide a method for producing the organic selenium mushroom rice, to improve the income of cultivated farmers and to greatly contribute to human health.

A further object of the present invention is to easily consume a crop containing organic selenium that is naturally biosynthesized through daily diet, supplying energy to the human body and at the same time preventing or preventing various diseases that may occur. The present invention provides a method of cultivating a crop containing a high concentration of organic selenium that can be treated.

It is another object of the present invention to provide a method for biosynthesis of organic selenium in various crops at low cost and high concentration in a short time.

It is still another object of the present invention to provide a crop containing organic selenium having a high concentration of organic selenium in a state in which a second biological treatment of crops or imported crops grown on general soil to contain organic selenium at a high concentration is performed. It is.

The present invention provides, in one aspect, a crop containing an organic selenium obtained by culturing a composite mushroom bacterium on a crop to which selenium is added, and a method of cultivating the crop so that the organic selenium is contained in the crop.

The method of allowing selenium to be contained in crops in an organicized form using mushrooms was first developed in Patent Application No. 10-2004-0099915 (2004.12.01), and the present inventors further improved it to use a composite mushroom bacterium. It has led to the further development of the culture system according to the present invention to further advance the conditions for organizing the organic selenium in agricultural products.

That is, the present invention is characterized by converting the inorganic selenium into organic selenium through biological metabolism by using the mixed mushroom bacteria and cereal grains such as rice and use as a nutrient of the mushroom, by this technology functional rice, black medicinal beans It is possible to produce various products such as organic selenium miso, organic selenium red pepper paste, organic selenium raw material, as well as soybeans, soybeans, grains and mixed grains.

Hereinafter, the present invention will be described in more detail.

As used in the present specification, the term "crop" refers to any edible or medicinal crop having commercial properties that are cultivated in an agricultural or biological manner, for example, fruits, horticulture, vegetables, as well as cereals. Include all of them. Therefore, in a specific embodiment of the present invention will be described for a specific crop, but the present invention is not limited to these, for example, rice, brown rice, brown rice glutinous rice, black rice, cereals, barley, soybeans, apples, pears, strawberries It is obvious that it includes tomato, bellflower, ginseng, and the like.

The method of providing an organic selenium-containing composite crop according to the present invention is inoculated with the mixed mushroom bacteria in the cultivation process of already grown crops or crops, followed by cultivation by adding an inorganic selenium solution thereto, or a solution containing selenium to the crops. After incorporating the compound mushroom bacteria, it is possible to convert the inorganic selenium into organic selenium through the culturing process to provide the consumer with a composite crop containing organic selenium (eg mushroom + rice). The addition form of the selenium may be added in the form of a powder, but it may be more preferable to add the liquid in the mushroom absorbed. In the case of providing the inorganic selenium as a solution, it is appropriate to use the inorganic selenium solution at a concentration of 80 to 120 ppm, particularly preferably 100 ppm, and more preferably to add the inorganic selenium at the same concentration 2-3 times during the culture. . As a result of the test of the present inventors, the addition of inorganic selenium to the final concentration of 80 ppm or less leads to a very low conversion rate of organic selenium, and to the addition of more than 120 ppm of inorganic selenium, it may cause problems in the conversion of organic selenium and in the growth of microorganisms. Can be. When the growth time is more than 120ppm, the growth period is long, and it is confirmed that only a part of the fungus grows, not the whole.

The mushroom bacteria used in the present invention is not particularly limited, but the complex mushroom bacteria are more preferable than the mushroom mushrooms alone, and it is most preferable to use chaga, situation mushrooms, and pine mushrooms in particular. And malt extraction liquid medium). Crops are most suitable grains as possible, but the present invention is not limited to a specific crop. Complex mushrooms are suitably inoculated in an amount of 5 to 15% based on the total volume. Cultivation is preferable to stationary culture rather than shaking culture.

Therefore, in the specific embodiment of the present invention,

a) Add malt to purified water and saccharify overnight at 55 ~ 66 ℃, inactivate enzyme for 20-30 minutes at 95 ~ 110 ℃, filter, and sterilize by adjusting the sugar content of extract to 10 ~ 13 brix Preparing malt extract liquid medium after treatment,

b) obtaining the respective liquid spawn by purely cultivating the situation mushroom, chaga and Pleurotus eryngii bacteria at 15-37 ° C. for 7-14 days in the malt extract liquid medium,

c) sterilizing the cleanly washed cereals (brown rice, wheat, beans) in a culture vessel;

d) filtering and sterilizing the inorganic selenium solution using a 0.15-03 μm filter,

e) adding 80 ~ 120ppm of filtered sterilized inorganic selenium solution to the cereals,

f) inoculating the mixed medium with 10-15% of each mushroom spawn purely cultured,

g) incubating the inoculum at 25 to 28 ° C. for 20 to 30 days,

h) culturing by adding 100ppm of inorganic selenium solution to the medium in two times of the culturing process of step g)

i) shaking the culture bottle once every 2 to 3 days after the inoculation mushroom mycelium begins to grow on the rice surface as a whole, and prevents the rice from clumping, and

j) After completion of the culture step of drying after freeze drying or drying using 65 ~ 85 ℃ hot air dryer

It provides a method for producing an organic selenium-containing mushroom rice comprising a.

If the inorganic selenium remains unconverted to organic selenium, the microorganism will not be cultured. Since microorganisms are not higher organisms, such as mammals or humans, if they are not suitable for themselves, they do not become cultures themselves.

In general, it is possible to expect the prevention of various diseases by ingesting organic selenium through the diet, but also considering that crops grown on ordinary soils have very small amounts of organic selenium, so its effect is difficult to expect. Inventions have been made.

The price of agricultural products is falling gradually, and the import of low-quality, high-quality foreign crops is increasing. Under these circumstances, selenium-containing crops can exhibit sufficient value in the market for cheap foreign produce, and even if foreign produce is imported, it can be sufficiently competitive.

According to the present invention, a technique of applying an inorganic selenium solution to a plant and converting it into organic selenium in the plant through the metabolic process of the mushroom may be applied to crops already grown, but may also be applied to crops being grown.

Accordingly, the present invention further provides a high concentration of organic selenium in a crop, which is characterized by increasing the content of organic selenium in the crop by repeatedly applying the inorganic selenium solution 1 to 20 times according to the growing season of the crop depending on the growing environment of the crop. To provide an accumulation method.

<Example>

Hereinafter, the present invention is described in more detail by the following representative examples, but the present invention is not limited in any way by these examples.

Example 1

250 g of malt oil was added to 1 liter of purified water and saccharified overnight at 60 ° C., and then incubated at 100 ° C. for 25 minutes to inactivate the enzyme, followed by filtration. To prepare, here, the mushrooms, chaga, and Matsutake mushrooms were purely incubated at 27 ° C. for 10 days to obtain a liquid seed. 350 g of brown rice was washed clean, soaked for about 8 hours, and then sterilized at 121 ° C. for 60 minutes in a 1 L plastic culture container. After cooling to room temperature, an inorganic selenium solution sterilized by filtration using a 0.2 μm filter was added to a concentration of 100 ppm. The selenium was well shaken to mix evenly and then inoculated with 10% of the pure cultured mushrooms and incubated at 27 ° C. for up to 30 days. After inoculation, the mycelia began to grow on the rice surface as a whole, shaking the culture bottle every three days to prevent rice from clumping. 100 ppm of the inorganic selenium solution was added to the medium twice in the culture process. After completion of the cultivation, the organic mushroom selenium-containing composite rice was prepared by drying after screening using a freeze 75 ℃ hot air dryer.

Example 2

In the same manner as in Example 1, was applied to brown rice, black rice, soybeans, black weak beans, mixed grains and the like and cultured for 15 days.

Test Example 1: Organic Selenium Content Test

In order to confirm the selenium content in the composite mushroom rice according to Example 1, the results were analyzed by the Korea Basic Science Institute (Analyzer-ICP / MS), the organic selenium content was significantly increased according to the treatment. The results are shown in Table 1 below. Selenium was not detected in the control group not treated with selenium.

Organic Selenium Content (mg / kg) Experiment 0.075 Control   Not detected

As described above, according to the present invention, by producing organic selenium-containing crops and providing biosynthesis of organic selenium at low cost and high concentration within a short time, the chemical method is eliminated, and minerals are efficiently treated through plant metabolism. By being absorbed into the plant, it is possible to provide minerals in a safe manner without adverse effects on the human body, and also to easily ingest nutrients from the crops, to exhibit the pharmacological effects represented by the organic selenium, thereby contributing to the improvement of health, and the organic selenium By making it contain, it is possible to combine the functional and palatability of a crop, and to provide a crop.

Claims (2)

a) Add malt to purified water and saccharify overnight at 55 ~ 66 ℃, inactivate enzyme for 20-30 minutes at 95 ~ 110 ℃, filter, and sterilize by adjusting the sugar content of extract to 10 ~ 13 brix Preparing malt extract liquid medium after treatment, b) obtaining the respective liquid spawn by purely cultivating the situation mushroom, chaga and Pleurotus eryngii bacteria at 15-37 ° C. for 7-14 days in the malt extract liquid medium, c) sterilizing the cleanly washed cereals in a culture vessel; d) filtering and sterilizing the inorganic selenium solution using a 0.15-03 μm filter, e) adding 80 ~ 120ppm of filtered sterilized inorganic selenium solution to the cereals, f) inoculating the mixed medium with 10-15% of each mushroom spawn purely cultured, g) incubating the inoculum at 25 to 28 ° C. for 20 to 30 days, h) culturing by adding 100ppm of inorganic selenium solution to the medium in two times of the culturing process of step g) i) shaking the culture bottle once every 2 to 3 days after the inoculation mushroom mycelium begins to grow on the rice surface as a whole, and prevents the rice from clumping, and j) After completion of the culture step of drying after freeze drying or drying using 65 ~ 85 ℃ hot air dryer Organic selenium-containing mushroom rice production method comprising a. Agricultural products prepared according to the method of claim 1.