KR101943219B1 - Production method of ganoderma lucidum culture medium - Google Patents

Abstract

According to one embodiment of the present invention, a method to produce medium for Ganoderma lucidum comprises: a step of preparing spawn for proliferating and cultivating Ganoderma lucidum spawn; a step of making the medium by mixing oak sawdust, cotton seed meal, beet pulp, rice bran, calcium carbonate, and tannin; a step of adjusting the moisture content of the medium at 60 to 70%; a step of bottling the mixture in a container to seal the container; a first sterilization step of putting the medium completing bottling in a sterilizer to perform sterilization at 115 to 125°C for 70 to 90 minutes; a second sterilization step of performing sterilization at 95 to 105°C for 5 to 8 hours after the first sterilization step; a step of cooling the medium completing second sterilization at 20 to 25°C; a step of inoculating Ganoderma lucidum spawn into the cooled medium in a sterilized chamber; and a step of cultivating the medium completing inoculation in a space of the temperature of 20 to 25°C and the moisture content of 65 to 75% for 4 to 5 weeks. In the medium making step, the medium is made by screening foreign matters or rough particles by a mesh with a grid of 3 to 5 mm after mixing of the oak sawdust, the cotton seed meal, the beet pulp, the rice bran, the calcium carbonate, and the tannin. The present invention is able to cultivate Ganoderma lucidum with improved quality.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for producing a mushroom culture medium containing ganoderma lucidum,

More particularly, the present invention relates to a method for producing gingko mushroom culture medium. More particularly, the present invention relates to a method for producing gingko mushroom culture medium, which comprises mixing and compressing oak sawdust, And a method for manufacturing the improved mushroom culture medium.

Ganoderma lucidum is widely distributed in the northwestern temperate zone of the northern hemisphere and is widely used in Korea, China, and Japan.

The surface of the gut is yellowish white at first, but when it grows, the color changes from the part where it grew up first to the color of the color change to the dark brown color.

The back of the shade is yellowish white with many holes and the stem is slightly curved with the same color as the surface of the shade. Ganoderma lucidum is a host of broad-leaved trees and occurs in the stubble area of the broad-leaved tree in the period from June to September. In addition, Ganoderma lucidum contains various components. Polysaccharide and polysaccharide protein conjugate and bitter taste terpeneid are the main active ingredients and have been reported to have effects on hypertension, immunity, and anticancer activity.

On the other hand, Ganoderma lucidum has excellent pharmacological properties and has been used in oriental medicine since ancient times. However, it has been used mainly as herbal medicine because there is not a lot of natural extracts and artificial cultivation technique has been popularized.

The present invention intends to develop a new high-value-added source of income by applying a medium capable of cultivating Ganoderma lucidum for the purpose of maximizing the preference according to the pharmacological properties of Ganoderma lucidum and further commercialization with high added value.

The present invention, which is devised to solve the above-mentioned problems, is a method for cultivating mushroom germ, which comprises mixing and compressing oak sawdust, cotton seed beet and beet pulp having a high moisture content, And a manufacturing method thereof.

In order to achieve the above object, the present invention provides a method for producing gingko mushroom culture, comprising the steps of: Oak sawdust, cottonseed, beet pulp, rice bran, calcium carbonate, and tannin to form a medium; Adjusting the moisture content of the medium to be 60 to 70%; Placing the medium in a container and sealing it; A primary sterilization step of putting the above-grounded medium into a sterilizer and sterilizing at 115 to 125 DEG C for 85 to 95 minutes; A secondary sterilization step for sterilization at 95 to 105 DEG C for 6 to 8 hours after the primary sterilization step; Cooling the secondary sterilized medium to 20 to 25 캜; Inoculating said cooled medium with said gingival extract in a clean room; Culturing the inoculated medium in a space at a temperature of 20 to 25 DEG C and a humidity of 65 to 75% for 4 to 5 weeks, wherein the step of producing the medium comprises the steps of: preparing the oak wood sawdust, cotton seed beet, beet pulp, , Calcium carbonate, and tannin, and then filtering the impurities or coarse particles using a sieve having a grid of 3 to 5 mm, followed by producing a culture medium.

In addition, the step of producing the medium may further comprise: 70 to 80 parts by weight of oak sawdust, 8 to 15 parts by weight of cottonseed, 10 to 15 parts by weight of beet pulp, 20 to 30 parts by weight of rice bran, 0.15 to 0.25 parts by weight of calcium and 0.5 to 1.5 parts by weight of tannin are added.

In addition, the step of culturing may include a step of ventilating for 15 to 20 minutes per day.

In addition, the rice bran is used after removing crushed rice bran by using a sieve with a grid of 0.5 to 1 mm.

In addition, the step of culturing may include a step of irradiating light of about 50 to 450 lux at the time of growing the fruiting body.

Also, the oak sawdust is characterized in that the acidity is maintained at pH 4.2 to 5.3.

In addition, the step of producing the culture medium includes a step of blending the nutrient mixture into the culture medium, wherein the nutrient mixture is selected from the group consisting of sugar, corn powder, corncob, cowpea, sorghum, buckwheat, bamboo, Or more.

Further, after the step of culturing, the method further includes the step of periodically spraying the vinegar to prevent the generation of insects, wherein the vinegar solution comprises the steps of: (a) burning wood to generate smoke; (b) recovering the vinegar produced by cooling the smoke; (c) aging the recovered vinegar using a specific gravity separation method, and (d) adding a growth promoter to the aged vinegar.

The growth promoter is characterized in that it contains at least one of oxine, gibberellin, cytokinin, triacontanol, ethylene, jasmonic acid, brassinosteroids, salicylic acid, taurine, .

The method for preparing gingko mushroom culture according to an embodiment of the present invention comprises mixing and compressing oak sawdust, cotton seed beet and beet pulp having a high moisture content, cultivating the medium by inoculating the mushroom seeds of Gingko mushroom and improving the quality by encapsulation cultivation method You can grow gingko mushrooms.

In addition, it can contribute to increase income of the farm household.

FIG. 1 is a flowchart illustrating a method for manufacturing a Ganoderma lucidum culture medium according to an embodiment of the present invention.
2 is a flow chart showing a method for producing a vinegar solution.

Hereinafter, the description of the present invention with reference to the drawings is not limited to a specific embodiment, and various transformations can be applied and various embodiments can be made. It is to be understood that the following description covers all changes, equivalents, and alternatives falling within the spirit and scope of the present invention.

In the following description, the terms first, second, and the like are used to describe various components and are not limited to their own meaning, and are used only for the purpose of distinguishing one component from another component.

Like reference numerals used throughout the specification denote like elements.

As used herein, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. It is also to be understood that the terms " comprising, "" comprising, "or" having ", and the like are intended to designate the presence of stated features, integers, And should not be construed to preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a flowchart illustrating a method for producing a Ganoderma lucidum culture medium according to an embodiment of the present invention.

Referring to FIG. 1, the method for preparing a gingko mushroom culture according to an embodiment of the present invention includes preparing a seed culture (S100) for growing and culturing the mushroom seedlings, oak sawdust, cotton seed beet pulp, rice bran, calcium carbonate, (S200), adjusting the moisture content of the medium to 60 to 70% (S300), placing the medium in the container (S400), performing a primary sterilization step (S500 (Step S600), cooling the mixture to 20 to 25 캜 (S600), inoculating the mung bean seedlings in a clean room (S700), heating the mixture to a temperature of 20 to 25 캜, a humidity of 65 to 75% Culturing for 5 weeks (S800), periodically spraying the woody liquid (S810) and harvesting the mung bean (S900) to prevent pests from occurring.

Specifically, the seedling preparation step (S100) for growing and culturing the myrtle seedlings is generally a step of preparing the seedlings of the myrrh.

Next, a step (S200) of producing a medium by blending oak sawdust, cottonseed, bit pulp, rice bran, calcium carbonate, and tannin is carried out in such a manner that 70 to 80 parts by weight of oak sawdust, 20 to 30 parts by weight 0.15 to 0.25 parts by weight of calcium carbonate, and 0.5 to 1.5 parts by weight of tannin.

The oak sawdust is one of the medium compositions commonly used in mushroom cultivation, plays a role of supplying water and oxygen, and maintains the humidity suitable for growth of the mushroom.

Also, oak sawdust accounts for 70 to 80 parts by weight of the whole medium. When the amount of oak sawdust is less than 70 parts by weight, the growth rate of about 2 weeks is slowed and the taste and flavor of the mushroom can not be sufficiently obtained. If the amount is more than 80 parts by weight, the growth rate of about 3 weeks may be slowed down.

Cotton seeds can be obtained after peeling off cottonseed which is a by-product of cotton industry, or by squeezing oil or extracting oil.

If the amount of cottonseed meal is less than 8 parts by weight, the content of the wood fiber is insufficient. Therefore, the condition is such that the mushroom can not grow sufficiently, If the amount is more than 15 parts by weight, the growth of the mushroom does not become more favorable, and thus it may be inefficient to use more than 15 parts by weight.

Beet pulp can be produced by extracting sugar from beet and using the remaining by-products directly, or by pelletizing, and can be used for feed.

In addition, the beet pulp occupies 10-15 parts by weight of the whole medium. When the amount of beet pulp used is less than 10 parts by weight, the mushroom can not grow sufficiently because the component of the wood fiber is insufficient. If the amount is more than 15 parts by weight, the growth of the mushroom does not get better, and therefore, it may be inefficient to use more than 15 parts by weight.

For reference, the weight of the fruiting body of the medium is normal but the weight of the fruiting body of the medium is increased by 10% to 20% when cotton patch is added, and in the case of beet pulp, But it is not effective when it is added in an amount exceeding 15 parts by weight.

It is known that rice bran is rich in beta-sterols and phytic acid (IP6), which have anti-cancer effects. The rice bran can act as a nutrient for the growth of mushrooms and can further improve the quality of mushrooms.

In addition, the amount of the raw steel is preferably 20 to 30 parts by weight

At this time, if the rice bran is less than 10 parts by weight, the growth rate of the mushroom may be slowed down. If the rice bran is more than 20 parts by weight, the growth rate of the mushroom does not improve more. Therefore, it may be inefficient and waste of materials.

Calcium carbonate improves the quality of mushrooms and improves the quality of mushrooms.

In addition, the calcium carbonate plays a role of adjusting the pH of the acid so that the acidity of the batch in which the water content is adjusted is maintained at pH 4.2 to 5.3, thereby lowering the disposal rate of the mushroom and sufficiently supplying calcium, .

If the amount of calcium carbonate used is less than 0.15 part by weight, calcium carbonate can not be supplied sufficiently, resulting in a high disposal rate. When the amount of calcium carbonate used is more than 0.25 part by weight In case of excessive supply of calcium, the growth of mushroom is unstable, so using more than that may be inefficient.

Tannin is widely distributed in plants and has a function of water-soluble protein to bind and denature.

As a result, tannins can help grow mushrooms.

In addition, tannin accounts for 0.5 to 1.5 parts by weight of the whole medium. If the amount of tannin used is less than 0.5 part by weight, it can not help growth of the mushroom. If the amount of tannin exceeds 1.5 parts by weight, , So using more than that can be inefficient.

In addition, it may include blending the nutritional mixture into the medium produced in step S200.

The nutritional mixture may include one or more of sugar, corn powder, corn cob, cow brew, sorghum, buckwheat, bamboo, coffee grounds.

The nutritional mixture may occupy from 5 to 10 parts by weight in the total weight portion of the medium.

Sugar is used as a carbon source. In other words, when the plant tissue is cultured, the chloroplast is not developed and the ability of photosynthesis decreases remarkably. Sugar is used to improve it.

Further, glucose can be used instead of sugar, but it is preferable to use low-priced sugar because it is expensive.

Corn powder has high starch content of 80% or more and high fat content in embryo, while low crude fiber content and good specularity are good for growth of mushroom hyphae.

The corncob is also known as cornstalks, and it supplies vitamin D to the medium to enable the cultivation of high quality mushrooms.

Clove leaves are the fruits of the Kapok tree, a tropical plant belonging to the Pannaceae family. They are made of filamentous fibers and contain yellowish green, aromatic and tasty oil.

The leaves remaining after milking the oil can be used for mushroom culture.

Sugarcane can act as a carbon source, like sugar, and can help mushrooms grow.

Buckwheat has the effect of increasing mycelial survival rate of mushroom seeds and decreasing contamination rate during cultivation.

In addition, the present invention can be applied to a method for producing a large amount of mushroom mycelium by improving productivity of mushroom mycelium.

Bamboo can be used after being crushed with a chipper and fermented for 1 month in a bulk bag.

It can also be used in place of sawdust and contains moisture, which helps in mushroom growth.

The coffee grounds are rich in nutrients needed to grow plants such as nitrogen, phosphorus, and potassium, and can be used for badges.

In step S200, impurities or coarse particles may be filtered by using a sieve having a grid of 3 to 5 mm formed by mixing oak sawdust, cottonseed, beet pulp, rice bran, calcium carbonate, and tannin.

That is, when the culture medium is produced by sieving as described above, it is possible to mechanize the material when mixing the material or when it is poured, and it is easy to drill holes in the inside of the bottle.

Punching is to make pores later on.

In step S200, rice bran is preferably used after removing crushed rice grain using a sieve with a grid of 0.5 to 1 mm.

In other words, it is necessary to remove and use crushed rice grain which is not nutritionally valuable, so it can be superior in terms of nutrition when mixing other ingredients.

Next, the step S300 is a step of adjusting the moisture content of the culture medium to 60 to 70%, and the water can be supplied so that the humidity (moisture) can be 60 to 70% by using a hygrometer.

That is, the amount of moisture required for growing the mushroom is less than 60%, the mushroom is difficult to grow, and when it exceeds 70%, the mushroom may grow and become rotten by the moisture.

Therefore, a moisture content of 60 to 70% is appropriate.

The step S400 may be a step of inserting the medium into the container and sealing the container.

After placing the medium in a PP bag, make the top surface flat and make a hole about 2 cm in diameter in the center of the medium.

Next, in step S500, the medium charged with the primary sterilization step may be placed in a sterilizer and sterilized at 115 to 125 DEG C for 70 to 90 minutes.

Next, the step S510 may be sterilized at 95 to 105 DEG C for 5 to 8 hours in a secondary sterilization step.

Specifically, the primary and secondary sterilization steps (S500 and S510) are sterilization, which is most important for the growth and culture of all microorganisms as well as mushrooms. Since all the processes are exposed to nature, there are many kinds of bacteria in the medium.

In order to grow only desired mushroom bacterium, it is necessary to kill existing bacterium through sterilization, soften the medium by optimum sterilization condition and time, prevent degradation of general ingredients into soluble state and destruction of main ingredient, It is important to maximize.

Generally, the mycelium is killed at about 70 ° C during sterilization. However, pathogenic bacteria causing blue mold, black mold, and red bread mold are more resistant to heat than ordinary molds. It is known that it does not die well at high temperatures.

Bacillus spp., A spore forming bacterium, has a very high heat resistance. Bacillus slearothermophilus should be killed for 90 to 90 minutes at 121 ° C. Sterilization at atmospheric pressure can be completely sterilized by keeping it at about 95 ° C for 5 to 8 hours. It is less evaporation of medium water, has good mycelial growth from the early stage of cultivation, and can soften the solid material of sawdust medium easily.

The step S600 is a step of cooling the secondary sterilized medium to 20 to 25 DEG C, transferring the sterilized hot medium to the cooling chamber, cooling the medium to a temperature of 20 to 25 DEG C overnight, and transferring it to the seedling chamber when the medium temperature is low .

Cooling the sterilized medium is a natural cooling, which can be contaminated with germs that can be produced at high temperatures, so it is transferred to a cooling chamber and cooled in a short time.

Next, the step S700 is a step of inoculating the mung bean seedlings in the clean room. It is preferable to inoculate the mung bean seedlings in the clean room for the mushroom seeds susceptible to the fungal infection.

At this time, it is preferable to inoculate about 6 to 7 g of the mung bean seedlings.

In the step of culturing for 4 to 5 weeks in a space having a temperature of 20 to 25 DEG C and a humidity of 65 to 75% at step S800, the culture room may be maintained at 20 to 25 DEG C to cultivate the mung bean germ.

If the temperature is higher than 25 ° C during the cultivation process, the temperature of the culture medium may rise due to the self heat generated in the culture medium, so that mycelial growth may be suppressed or stopped.

Next, in order to prevent the occurrence of insects, step S810 of periodically spraying the wood vinegar can be performed.

Mushroom seeds may be damaged by insect pests or inoculated seeds, which can be sprayed to prevent them.

Wood vinegar is used as a substitute for pesticides because smoke generated when trees are made into charcoal is liquefied and dropped while coming in contact with the outside air.

Referring to FIG. 2, a method of producing a wood vinegar solution comprises the steps of burning wood to generate smoke (S10), recovering the vinegar solution produced by cooling the smoke (S20), using the recovered wood vinegar (S30), and a step (S40) of blending the aged vinegar with a growth promoting agent.

The steps S10 to S20 of the step of producing the vinegar solution are the steps that are typically manufactured, and a detailed description thereof will be omitted.

In the step S30, the specific gravity separation method is based on the difference in weight. Although it takes more than 6 months, it can be mass-produced and is effective in removing harmful substances.

Also, the cost is low.

Specifically, when the vinegar solution is placed in a container for a long period of time, it is separated into three layers of the upper layer. The vinegar solution in the middle layer is the vinegar solution. And the liquids dissolved in the oil in the upper and lower layers are respectively 'light oil quality' and 'tar'.

It is recommended to thoroughly separate the tar component after aging and refining for several months to one year after harvesting. This is because the tar component contains harmful substances such as cresol.

Woody vinegars in the middle layer contain harmful substances such as wood tar, phenol, methanol, cresol, and benzolpyrene, which can be used as raw materials for agriculture, animal husbandry, plants, medicines and the like.

In order to be used for food, it is necessary to remove all harmful components through a special process, which is thought to be the know-how to make edible vinegar.

The original vinegar shows a clear amber color (whiskey color), but the vinegar, which completely eliminates harmful components such as wood tar, becomes a transparent liquid similar to a vinegar with a light yellow color.

Step S40 is a step of blending the growth promoter with the prepared vinegar solution.

The growth-promoting agent may include one or more of oxine, gibberellin, cytokinin, triacontanol, ethylene, jasmonic acid, brassinosteroids, salicylic acid, teroglycines and Strigol.

The auxin was isolated from the immature corn seeds by the conversion of indole acetic acid, the natural auxin.

Auxin is similar to the structure of tryptophan, one of the amino acids, and tryptophan acts as precursor to auxin. Auxin expands cells through cell growth and is involved in the induction of leaf tearing, root shearing, and root differentiation.

Gibberellin is a plant growth hormone. As a result of aseptic treatment of the culture medium of Gibella fujikuroi, a microorganism causing rice seedling disease, in rice seedlings, not only the growth of rice was promoted but also the formation of chloroplasts and the development of roots were inhibited .

In addition, gibberellin is abundantly contained in higher plants and has physiological activities such as inactivation of hydrolase, promotion of flowering, and promotion of fruit production.

Cytokinin is a compound that causes cell division in tobacco water tissues or incubation breakfasts (in addition to tobacco callus, soybean callus, carrot secondary tubule, etc.) in the presence of auxin at an optimal concentration, and exhibits similar action to cannesin for other physiological functions.

Triacontanol promotes the germination of seeds and promotes the division and traction of plant cells.

It is also effective in energy storage and facilitates the accumulation of materials.

It is also stable to light, air, and base.

Ethylene is known to regulate the expression of a wide range of physiological features in almost all plant tissues.

In the case of other plant hormones, for example, promoting postnatal growth of fruit, triple reaction of pea sulphation (inducing horizontal growth of the stem, inhibiting the growth of the kidneys and growth of the kidneys), inducing growth of the petiole For example.

A compound other than ethylene, which exhibits an ethylene action, is propylene, which has the highest activity, but its activity is said to be about 1/130 of ethylene.

And it is said that production of propylene is not confirmed in plant tissue.

Jasmonic acid is a cyclopentanone compound widely distributed in the vegetable field and is a group of substances related to jasmonic acid.

Jasmonic acid is synthesized from linolenic acid and is found in higher plants, algae and microorganisms.

It inhibits growth of oily plants, inhibits cell growth and proliferation, stimulates cell growth, induces rooting, promotes tuber formation, promotes senescence, inhibits seed and pollen germination.

Brassinosteroids were first isolated from the pollen of rape collected by bees as a natural plant growth regulator with a steroid structure.

In other words, it can interact with light to regulate plant growth.

Salicylic acid is a plant phenolic found in higher plants that exist everywhere, and when applied to a plant, it induces flowering, increased resistance to plant pathogens, and the like.

내지

농도에서 잎의 운동을 촉진한다.Turgolin was isolated from acacia as a substance related to the spontaneous movement of plants according to the surrounding environment,

To

Promotes movement of the leaves at the concentration.

Strigol has been the first to separate from the exudates of cotton roots as a substance that promotes seed germination.

In addition, the step S800 may include a step of ventilating for 15 to 20 minutes per day, and a step of irradiating 50 to 450 lux of light during the growth of the fruit body.

The step of irradiating the ventilation and light to the light helps to cultivate high quality mushrooms by supplying a little air change and light to the medium cultured in an airtight space.

The step S900 is the step of harvesting cultivated Ganoderma lucidum. The first mushroom to be harvested is the best, and the mushrooms harvested after that can be harvested faster than the first cultivated mushroom and shorten the time to cultivate.

That is, the first mushroom to be harvested can be harvested after about 4 to 5 weeks of cultivation, and the next mushroom can be harvested after about 7 to 8 days.

Hereinafter, the present invention will be described in more detail with reference to Production Examples and Experimental Examples. However, the following Examples are for illustrative purposes only and are not intended to limit the present invention.

[Example]

A medium was prepared by mixing 75 parts by weight of oak sawdust having an acidity of pH 4.8, 10 parts by weight of cottonseed powder, 15 parts by weight of beet pulp, 25 parts by weight of rice bran, 0.2 part by weight of calcium carbonate, 0.1 part by weight of tannin, , And the prepared medium was filled in the bag. At this time, the nutrient mixture is a mixture of sugar, corn powder, corn cob, cow brew, sugar cane, buckwheat, bamboo, and coffee grounds. The bag was placed in a sterilizer and placed in a sterilizer and sterilized at 120 ° C for 90 minutes. After the primary sterilization, after the secondary sterilization at 100 ° C for 7 hours, the temperature of the medium was 23 ° C After cooling in a cooling chamber, seedlings were inoculated and sprayed with wood vinegar. In this case, the vinegar solution is a mixture of auxin, gibberellin, cytokinin, triacontanol, ethylene, jasmonic acid, brassinosteroid, salicylic acid, taurine and strygol. Thereafter, the cells were cultured and harvested for 5 weeks in a room at 23 ° C and a humidity of 70%.

The culture was ventilated for 10 minutes a day for 5 weeks, and a step of irradiating 300 Lux mines for 5 minutes a day was performed.

Also, the medium was filtered with impurities or coarse particles using a sieve with a grid of 4 mm, and the rice bran was cultivated with a step of removing crushed rice grains using a sieve with a grid of 0.8 mm.

[Comparative Example 1]

Except that the acidity of the oak sawdust in the above example was adjusted to pH2.2.

[Comparative Example 2]

Ganoderma mushroom was cultivated in the same manner as in the Example except that the culture medium of the above example and the structure in which the rice bran were not sieved.

[Comparative Example 3]

Ganoderma mushroom was cultivated in the same manner as in the Example except that the steps of ventilation and diffuse light during the cultivation of the above Example were omitted.

[Comparative Example 4]

Ganoderma mushroom was cultivated in the same manner as in the example except that the nutritional mixture of the above example was composed of only sugar.

[Comparative Example 5]

In the composition for spraying the vinegar solution of the above embodiment, gingival mushroom was cultivated in the same manner as in the Example except for spraying a commercially available vinegar solution.

The growth rate, production amount and size of Ganoderma lucidum according to the above Examples and Comparative Examples 1 to 5 are shown in Table 1.

Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Growth rate
(% / 25 days) 91 48 56 65 60 72 Production (g) 510 310 325 420 402 465 Freshly sized
(Diameter cm) 25 12 16 20 18 22 badge
Success rate (%) 97 55 65 72 68 82

As can be seen in Table 1, the growth rate of the Examples of the present invention was much higher than those of Comparative Examples 1 to 5, and the production amount and the shade size were much higher.

It is also confirmed that the medium success rate is higher than that of Comparative Examples 1 to 5.

Therefore, it was concluded that the cultivation of Ganoderma lucidum according to the present invention would enable stable, efficient and high quality cultivation of Ganoderma lucidum.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be clear to the person.

Claims (9)

A seed preparation step for proliferating and culturing the mung bean seedlings;
Oak sawdust, cottonseed, beet pulp, rice bran, calcium carbonate, and tannin to form a medium;
Adjusting the moisture content of the medium to be 60 to 70%;
Placing the medium in a container and sealing it;
A primary sterilization step of putting the above-grounded medium into a sterilizer and sterilizing at 115 to 125 DEG C for 70 to 90 minutes;
A secondary sterilization step in which after the primary sterilization step, sterilization is performed at 95 to 105 ° C for 5 to 8 hours;
Cooling the secondary sterilized medium to 20 to 25 캜;
Inoculating said cooled medium with said gingival extract in a clean room;
Culturing the inoculated medium at a temperature of 20 to 25 DEG C and a humidity of 65 to 75% for 4 to 5 weeks,
Wherein the step of generating the medium comprises:
Wherein the medium is produced by filtering the impurities or coarse particles using a sieve body having a grid of 3 to 5 mm after mixing the oak sawdust, cottonseed pulp, beet pulp, rice bran, calcium carbonate, and tannin. Way.
The method according to claim 1,
Wherein the step of generating the medium comprises:
The beverage according to any one of claims 1 to 7, wherein the whole of the medium is composed of 70 to 80 parts by weight of oak sawdust, 8 to 15 parts by weight of cottonseed, 10 to 15 parts by weight of beet pulp, 20 to 30 parts by weight of rice bran, 0.15 to 0.25 parts by weight of calcium carbonate, And 1.5 parts by weight of the composition.
The method according to claim 1,
The step of culturing
And ventilating for 15 to 20 minutes per day.
The method according to claim 1,
Wherein the rice bran is used after removing crushed rice grains using a sieve having a grid of 0.5 to 1 mm.
The method according to claim 1,
Wherein the culturing comprises:
And irradiating light of about 50 to 450 Lux at a time of growing a fruiting body.
The method according to claim 1,
Wherein the oak sawdust is produced such that the acidity is maintained at a pH of 4.2 to 5.3.
The method according to claim 1,
Wherein the step of generating the medium comprises:
Comprising combining the nutrient mixture in the medium,
The nutritional mixture may contain,
Sugar, corn powder, corn cob, cow brew, sugar cane, buckwheat, bamboo, coffee grounds.
The method according to claim 1,
After the step of culturing,
Further comprising the step of periodically spraying the wood vinegar to prevent the occurrence of pests,
The wood vinegar,
(a) burning wood to generate smoke;
(b) recovering the vinegar produced by cooling the smoke;
(c) aging the recovered vinegar using a specific gravity separation method and
(d) adding a growth promoter to the aged vinegar solution.
9. The method of claim 8,
The growth promoter,
A method for producing a Ganoderma lucidum culture medium characterized by comprising at least one of oxine, gibberellin, cytokinin, triacontanol, ethylene, jasmonic acids, brassinosteroids, salicylic acid, tacholines and streigol.

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