KR102233304B1 - Medium composition comprising a large amount of human immune improving and physiological active substance for stable cultivating vegetable worms and the method of cultivating vegetable worms using the medium and the vegetable worms cultivated by using the method - Google Patents

Abstract

The present invention relates to a culture medium composition of Cordyceps militaris, which can be cultured stably and contains a large amount of human immunity enhancement and physiologically active substances, to a method for cultivating Cordyceps militaris using the same, and to a Cordyceps militaris cultivated thereby and, more specifically, to a culture medium composition of Cordyceps militaris, which can be cultured stably and contains a large amount of human immunity enhancement and physiologically active substances by using the culture medium composition of Cordyceps militaris including brown rice powder, corn powder, pupa powder, spirulina powder, seaweed powder, and glucose in goat milk, to a method for cultivating Cordyceps militaris using the same, and to a Cordyceps militaris cultivated thereby.

Description

The cultivation medium composition of Militaris Cordyceps sinensis, which is capable of stable cultivation and contains a large amount of human immunity and physiologically active substances, and the method of cultivating Militaris Cordyceps sinensis using the same, and the Medium composition comprising a large amount of human immune improving and physiological active substance for stable cultivating vegetable worms and the method of cultivating vegetable worms using the medium and the vegetable worms cultivated by using the method}

The present invention relates to a cultivation medium composition of Militaris Cordyceps sinensis, which is capable of stable cultivation and contains a large amount of human immunity and physiologically active substances, and a method of cultivating Militaris Cordyceps sinensis using the same, and to Militaris Cordyceps sinensis cultivated accordingly, Specifically, stable culture is possible by using the cultivation medium composition of Militaris Cordyceps sinensis consisting of brown rice powder, corn powder, pupa powder, spirulina powder, seaweed powder, and glucose in goat milk, enabling stable culture and enhancing human immunity and physiologically active substances. It relates to a cultivation medium composition of Militaris Cordyceps sinensis containing a large amount of Militaris Cordyceps sinensis and a method of cultivating Militaris Cordyceps sinensis using the same, and Militaris Cordyceps sinensis cultivated accordingly.

In general, Cordyceps spp refers to a type of mushroom named because it is in the body of an insect in winter and appears like a grass in summer.The cordyceps spp is an insect group that occurs when cordyceps, a type of fungus, enters the body of a living insect. It is a medicinal mushroom produced, and insects infected with the Cordyceps sinensis are characterized by maintaining their shape like a mummy without decaying even if they die until the mushroom emerges.

About 300-400 species of cordyceps have been found around the world, and 80 species have been discovered in Korea. Representative insects that parasitize the cordyceps are bees, ants, dragonflies, butterflies, cicadas, stink bugs, beetles, flies, and spiders. In these insects, cordyceps are parasitic in the state of eggs, larvae, pupae, and adult insects.

The color of mushrooms generated by insects infected with cordyceps has various beautiful colors such as red, yellow, purple, green, black, white, orange, and olive, and cordyceps are very small in size and difficult to find because their habitat is difficult. It is difficult, and in most cases, is found in places where the air is clean, humid, moderately shaded, and in a natural state.

Representative cordyceps used for medicinal purposes to date include Chinese-made Cordyceps sinensis and Cordyceps militaris, which form fruiting bodies based on larvae. In particular, Militaris cordyceps are mainly caterpillars of the order Lepidoptea. Or it is a kind of entomopathogenic fungi that forms orange club-shaped fruiting bodies with pupa as the host.

In addition, Militaris Cordyceps sinensis contains relatively large amounts of cordycepine among several cordyceps, so its fruiting bodies are known to protect the lungs and strengthen the kidneys, and are widely recognized for their effects as a tonic and treatment for tuberculosis and jaundice. No, it is being used as an expensive herbal medicine.

That is, cordyceps has been recognized for a long time through Donguibogam, and has been used to treat various adult diseases and maintain health. In China, athletes take cordyceps and show miracle records, so cordyceps are recognized as one of the top three natural health foods.

On the other hand, cordyceps belong to the group with the highest protein content (more than 28% of dry content) among crops, and contain a large amount of cordycepin, a physiologically active substance that strengthens immunity, and the cordycepin is kinic acid. As a natural compound of the 3-deoxyadenosine system, which has been found to be an isomer of (quinic acid), it has been found in clinical trials to be effective in respiratory, immunity, fatigue recovery, anti-cancer, anti-inflammatory and diabetes.

In particular, among cordyceps cordyceps, Militaris cordyceps has a significantly higher content of cordycepin than other cordyceps. Cordycepin is a nucleic acid substance that participates in the genetic information of cells and activates a reduced immune function to prevent normal cells from being converted into cancer cells. It works to do.

In other words, it was confirmed that the content of NK cells (natural killer cells), which are immune cells that kill cancer cells, and cytokines secreted from immune cells, increased by 18% to 25% in a clinical trial in which Cordyceps sinensis was injected. It is used for use and is receiving a lot of attention as a health functional food.

Based on these results, in Korea, cordyceps containing cordyceps was the only mushroom that was certified by the Ministry of Food and Drug Safety as a health functional food. In addition, the results of clinical studies have been announced that cordyceps sinensis has the effect of controlling blood pressure and diabetes, as well as helping lung and liver functions.

As such, cordyceps is an important crop that is continuously studied in the domestic medicine and food fields because of its high value as a crop, and its importance is recognized not only as a medicine but also as a health supplement, and various powder or liquid products are currently on the market. Has become.

In addition, research to develop non-polluting microbial pesticides by using cordyceps to control the density of insects in the natural ecosystem is also active. It is attracting great interest because it can even solve environmental pollution problems caused by pesticides.

Like this, cordyceps are consumed a lot among mushrooms, but the amount collected from nature is very small and cannot meet the demand, so many farmers around the world have made cultivation houses using artificial cultivation methods and cultivated them in large quantities.

However, cordyceps is a plant that grows slowly and cannot spray pesticides because it is sensitive to the external environment, so it is very difficult to cultivate, and although it has succeeded in mass cultivation, it is known that the yield of cordyceps cultivation is low even in farms that have been cultivated for a long time. However, when 10 cordyceps are cultivated and harvested, about 1-2 are contaminated or abnormal strains grow, resulting in a lower harvest.

Currently, the most widely used methods for cultivation of cordyceps are the cultivation of caterpillar pupa cordyceps inoculated by directly inoculating the silkworm larvae, the cultivation method of brown rice + pupa sage cordyceps cultivation by inoculating bacteria in a medium mixed with silkworm pupa powder and brown rice. It is a cultivation method in which germanium components are strengthened in the cultivation method, and it is generally cultivated in a flat plate by inoculating in a sterilized state or cultivated in a bottle like other mushrooms.

Looking at the existing technologies related to the cultivation method of cordyceps like this, in Korean Patent Laid-Open Patent 10-2017-0076075 (published on July 4, 2017), a medium formation step of forming a medium containing germinated brown rice and organic germanium water, and the above A method of cultivation of cordyceps containing a high content of cordycepine and organic germanium, characterized in that it comprises a step of inoculating a seed of Militaris Cordyceps sinensis into a medium, and culturing the inoculated medium is known. Has been.

In addition, Korean Patent Publication No. 10-2017-0113825 (published on October 13, 2017), (a) preparing chaga mushrooms by pulverizing the size of 500㎛ ~ 900㎛; (b) preparing the mushrooms by pulverizing them to a size of 10 µm to 200 µm; (c) preparing horseshoe mushrooms by grinding them to a size of 100 μm to 500 μm; (d) Chaga powder of step (a): Pseudomonas mushroom powder of step (b): Prepared by mixing horseshoe mushroom powder of step (c) in a weight ratio of 4:4:2 to 5:4:1 The step of doing; (e) mixing 10g to 500g of the mixed powder of step (d) per 1 kg of drinking water and extracting for 1 to 2 hours at a temperature of 90°C to 100°C; (f) preparing an insect for a host by washing it with drinking water; (g) adding the host insect of step (f) to the material of step (e) and steaming for 1 hour to 3 hours at a temperature of 90°C to 100°C; (h) preparing by dehydrating the steamed host insect of step (g); (i) introducing the dehydrated host insect of step (h) into a culture vessel; (j) inoculating the cordyceps spp. in the host insect of step (i) and culturing them at a humidity of 80% to 95% and a temperature of 18°C to 30°C for 5 to 10 days; (k) cultivating the cultured host insect of step (j) at a humidity of 85% to 96% and a temperature of 20°C to 26°C for 7 to 15 days; (l) A method of cultivating high-quality Cordyceps sinensis using natural products, characterized in that it comprises the step of commercializing the cordyceps sinensis of step (k) to a moisture content of 15% or less.

In addition, in Korean Patent Registration No. 10-2062275 (registration date December 27, 2019), (a) straightening the fruiting body of Cordyceps at 85 to 90° C. for 100 to 140 minutes; (a') immersing the fruiting body of the flat cordyceps sinensis in water at 15 to 25° C. for 15 to 25 seconds; (b) steaming the cordyceps sinensis taken out after being immersed in water at 85 to 95° C. for 15 to 25 minutes with a non-pressure steamer; (c) drying the steamed cordyceps sinensis at 45 to 55° C. for 50 to 70 minutes by dehumidifying cold air drying; And (d) repeating steps (b) and (c) 3 to 5 times. A method for producing cordyceps with an increased content of cordycepine is known.

In addition, according to Korean Patent Publication No. 10-2019-0065916 (published on June 12, 2019), fulvic acid 01-20 parts by weight of 1 to 10 parts by weight of basic medium for cultivation of cordyceps at least one selected from brown rice, germinated brown rice, corn, and pupae. A medium composition for cultivation of cordyceps containing part and a method of cultivating cordyceps using the same are known.

In addition, in Korean Patent Laid-Open Publication No. 10-2020-0048736 (published date May 08, 2020), a mixture of brown rice medium containing Cordyceps sinensis is sequentially cultured at a first temperature of 3°C or less and a second temperature of 20°C or more. Including a culture step in which the shock cycle is repeatedly performed, the low temperature shock cycle, t1) the step of reducing the temperature of the brown rice medium mixture to the first temperature t2) the step of raising the temperature of the reduced brown rice medium mixture to the second temperature And t3) maintaining the heated brown rice medium mixture at the second temperature.

However, the conventional patent technologies generally relate to a method of cultivation using a known medium composition and controlling the cultivation conditions, and speeding the growth rate of cordyceps and the content of cordycepine contained in cordyceps is limited to the components of the medium composition. Therefore, there is a problem that there is a limit to increasing the content of cordycepine.

In addition, research on the development and cultivation technology of seed fungi to enhance the pharmacological components and efficacy of Cordyceps sinensis was being conducted in China, Korea, and Japan, but now it has become a mushroom of global interest, and competitive research is conducted in countries around the world. The present invention proposes a method of cultivation of Cordyceps sinensis, which can increase the growth rate of Cordyceps sinensis quickly and stably, and further increase the content of physiologically active substances contained in Cordyceps sinensis, and the Militaris Cordyceps plant grown accordingly. It is aimed at.

[Patent Document 001] Korean Patent Publication 10-2017-0076075 (Publication date July 4, 2017) [Patent Document 002] Korean Patent Publication 10-2017-0113825 (Publication date October 13, 2017) [Patent Document 003] Korean Patent Registration 10-2062275 (Registration Date December 27, 2019) [Patent Document 004] Korean Patent Publication 10-2019-0065916 (Publication date June 12, 2019) [Patent Document 005] Korean Patent Publication 10-2020-0048736 (Publication date May 8, 2020)

In order to solve the above problems, the present invention contains brown rice powder, corn powder, pupa powder, spirulina powder, seaweed powder, and glucose in goat milk to enable stable culture and enhance human immunity and contain a large amount of physiologically active substances. It is a task to solve the cultivation medium composition of Militaris Cordyceps sinensis and the cultivation method of Militaris Cordyceps sinensis using the same, and to provide the Militaris Cordyceps sinensis cultivated accordingly.

In order to solve the above problems, the present invention is composed of goat milk, including brown rice powder, corn powder, pupa powder, spirulina powder, seaweed powder, and glucose, enabling stable culture and enhancing human immunity and containing a large amount of physiologically active substances. The cultivation medium composition of Taris Cordyceps is used as a solution to the problem.

The cultivation medium composition further comprises sodium alginate solution as a solution to the problem.

The cultivation medium composition is based on 100 parts by weight of goat milk, 10 to 15 parts by weight of brown rice powder, 10 to 15 parts by weight of corn powder, 10 to 15 parts by weight of pupa powder, 5 to 10 parts by weight of spirulina powder, 5 to 10 parts by weight of seaweed powder It is a solution to the problem that is composed of parts, including 3 to 5 parts by weight of glucose.

In addition, the present invention, based on 100 parts by weight of goat milk, 10 to 15 parts by weight of brown rice powder, 10 to 15 parts by weight of corn powder, 10 to 15 parts by weight of pupa powder, 5 to 10 parts by weight of spirulina powder, 5 to 10 parts by weight of seaweed powder A medium forming step of forming a medium composed of 3 to 5 parts by weight of glucose; Inoculation step of inoculating the seed of Militaris Cordyceps sinensis into the medium; A first culture step of culturing for 10 to 15 days in a constant temperature and humidity culture room in a constant temperature and humidity culture room after the inoculation step for 10 to 15 days at a temperature of 25° C., a humidity of 80%, and an illuminance of 500 Lux; After the first culture step, a second culture step of lowering the temperature to 20° C. and culturing for 35 to 45 days in a culture condition of 80% humidity and 500 Lux of illuminance to form a fruiting body in a complete form; including, but In the secondary culture step, stable culture including the step of uniformly spraying sodium alginate solution to induce stable growth of the fruiting body tissue by forming ^{Ca 2+ and calcium alginate of the goat milk is possible, and human immunity enhancement and physiologically active substances} A method of cultivating Militaris Cordyceps sinensis using a cultivation medium composition of Militaris Cordyceps sinensis containing a large amount is a solution to the problem.

The solution to the problem is to further include a medium sterilization step of sterilizing the medium at 120 to 130° C. and 15 psi (11 kg/cm ^{2) for 15 to 30 minutes after the medium forming step and before the inoculation step.}

In addition, the present invention solves the problem of the Militaris Cordyceps sinensis cultivation method using the cultivation medium composition of Militaris Cordyceps sinensis, which is capable of stable cultivation and contains a large amount of human immunity enhancing and physiologically active substances. By means.

According to the cultivation medium composition of Militaris cordyceps sinensis, which is capable of stable culture of the present invention and contains a large amount of human immunity enhancing and physiologically active substances, and the cultivation method of Militaris Cordyceps sinensis using the same, and the Militaris Cordyceps plant grown accordingly, in goat milk As it is cultivated in a cultivation medium containing brown rice powder, corn powder, pupa powder, spirulina powder, seaweed powder, and glucose, stable culture is possible. It can have an excellent effect.

The present invention is composed of goat milk with brown rice powder, corn powder, pupa powder, spirulina powder, seaweed powder and glucose to enable stable cultivation, and cultivation medium of Militaris Cordyceps sinensis containing a large amount of human immunity enhancing and physiologically active substances The composition is characterized by the technical composition.

The cultivation medium composition is characterized in that the composition further comprises a sodium alginate solution.

The cultivation medium composition is based on 100 parts by weight of goat milk, 10 to 15 parts by weight of brown rice powder, 10 to 15 parts by weight of corn powder, 10 to 15 parts by weight of pupa powder, 5 to 10 parts by weight of spirulina powder, 5 to 10 parts by weight of seaweed powder Part, characterized in that the composition is composed of 3 to 5 parts by weight of glucose.

In addition, the present invention, based on 100 parts by weight of goat milk, 10 to 15 parts by weight of brown rice powder, 10 to 15 parts by weight of corn powder, 10 to 15 parts by weight of pupa powder, 5 to 10 parts by weight of spirulina powder, 5 to 10 parts by weight of seaweed powder A medium forming step of forming a medium composed of 3 to 5 parts by weight of glucose; Inoculation step of inoculating the seed of Militaris Cordyceps sinensis into the medium; The first culture step of culturing for 10 to 15 days in a constant temperature and humidity culture room after the inoculation step at a temperature of 25° C., a humidity of 80%, and an illuminance of 500 Lux for 10 to 15 days so that the mycelium is settled on the entire medium; After the first culture step, a second culture step of lowering the temperature to 20° C. and culturing for 35 to 45 days in a culture condition of 80% humidity and 500 Lux of illuminance to form a fruiting body in a complete form; including, but In the secondary culture step, stable culture including the step of uniformly spraying sodium alginate solution to induce stable growth of the fruiting body tissue by forming ^{Ca 2+ and calcium alginate of the goat milk is possible, and human immunity enhancement and physiologically active substances} A method of cultivation of Militaris Cordyceps sinensis using a cultivation medium composition of Militaris Cordyceps sinensis containing a large amount is characterized by the technical configuration.

After the medium forming step, before the inoculation step, the method further comprises a medium sterilization step of sterilizing the medium at 120 to 130° C. and 15 psi (11 kg/cm ² ) for 15 to 30 minutes.

In addition, the present invention is capable of cultivating the above stable cultivation and the cultivation medium composition of Militaris Cordyceps sinensis which contains a large amount of human immunity and physiologically active substances, and uses the Militaris Cordyceps sinensis cultivation method. It is characterized.

Hereinafter, embodiments of the present invention and/or drawings will be described in detail so that those of ordinary skill in the art can easily implement the present invention. However, the present invention may be implemented in various different forms, and is not limited to the embodiments and/or drawings described herein.

First, the cultivation medium composition of Militaris Cordyceps sinensis, which is capable of stable culture of the present invention and contains a large amount of human immunity enhancing and physiologically active substances, contains brown rice powder, corn powder, pupa powder, spirulina powder, seaweed powder and glucose in goat milk. At this time, the content of each component of the medium composition is based on 100 parts by weight of goat milk, 10 to 15 parts by weight of brown rice powder, 10 to 15 parts by weight of corn powder, 10 to 15 parts by weight of pupa powder, 5 parts by weight of spirulina powder. It is preferably composed of ~ 10 parts by weight, 5 to 10 parts by weight of seaweed powder, and 3 to 5 parts by weight of glucose.

Here, the goat milk has an energy of 63 Kcal/100 g, and is similar to breast milk or milk, and thus can replace breast milk. In particular, goat milk has very valuable value as a milk substitute for infants with poor nutrition in areas where milk is not produced or formula is too expensive, such as in developing countries.

In addition, the amount of goat milk that can be obtained from one goat grazing in a clean and wide grassland is 0.3~3L, contains a lot of protein, fat, and ash, and the ratio of albumin is high among proteins, and fat is less than milk fat, so it is easily digested. Even infants with malabsorption disorders in the intestine can drink without difficulty.

In particular, goat milk contains less alpha S1-casein, which is known as a milk allergen, than milk, so it is less likely to cause allergies or atopy, and contains more than 20 to 28% more selenium, an inorganic salt than milk.

Most of them drink in Korea and Japan, but in Europe it is often processed with cheese and butter, and nowadays there are many secondary processed foods as well as goat milk in Korea. One of the advantages of goat milk is to prevent skin aging and skin beauty. The effect is attracting attention and is gaining great popularity among women, and the action of the following four physiologically active ingredients of goat milk is a great help in enhancing human immunity.

First, goat milk has a high proportion of beta casein, unlike regular milk, and this beta casein has an immunity enhancing effect. Second, in general, milk is an acidic food, but goat milk is an alkaline food, so in order to increase immunity, the human body must also be alkalized. Therefore, goat milk, an alkaline food, is a great help in enhancing the body's immunity. Third, goat milk is the closest food to human milk, and it can be said to be the most suitable food for the human body.These goat milk contain the antioxidant components of breast milk, such as CLA, nucleotides, sphingomyelin, L-carnitine, and growth factor IgF. Helps immune function. Fourth, goat milk contains fats, carbohydrates, proteins, minerals, various vitamins and various physiologically active substances evenly compared to other foods, so it can be said to be a good food for the body. It helps strengthen the body's immunity.

As described above, components including alpha S casein, beta casein and gamma casein of goat milk are immune-boosting, anti-inflammatory and anti-allergic. (anti-allergy) Since it has the effect of showing an active effect, it is used as the basic composition of the cultivation medium composition of the Militaris Cordyceps sinensis of the present invention, thereby enhancing human immunity and a large amount of physiologically active substances in the cultured Militaris Cordyceps sinensis. It is a characteristic constitution of the present invention to contain.

In addition, unpolished rice is a pear whose structure occupies a small part of the rice bran layer and the base of rice grains, such as the peel, seed coat, and algae layer from the outside. ), and the endosperm, which accounts for most of the rest. This endosperm is mainly composed of starch particles, and is an edible part as white rice. The weight ratio of each of the above parts to the whole grains of brown rice is 5-6% of rice bran layer, 2-3% of pear, and 92% of endosperm. Therefore, when the brown rice is polished with a rice mill, 92% of brown rice can be obtained. The weight of 1 liter of brown rice is 770-840g, and the weight of 1,000 grains of brown rice is 18-21g for small grains, 21-24g for medium grains, 26-28g for large grains, and 1.36-1.44 specific gravity.

The standard chemical composition of the brown rice is water 15.5%, protein 7.4%, lipid 3.0%, carbohydrate 71.8%, fiber 1.0%, ash 1.3%, vitamin B1 is 0.54mg out of 100g. It occupies, there is not much protein or fat, and it has a lot of vitamin B1. Compared to white rice, brown rice has better storage and less damage to insects and microorganisms. In addition, brown rice is rich in fat, protein, and vitamin B1·B2 compared to white rice because there is no loss of nutrients due to refinement.

When the nutrients of sugar (carbohydrate) and vitamin B1 of brown rice as described above are used as a medium composition component of the Militaris Cordyceps sinensis of the present invention, Militaris Cordyceps sinensis is supplemented with nutrients of sugar (carbohydrate) and vitamin B1. In addition, the brown rice content of the medium composition of the present invention is preferably used in an amount of 10 to 15 parts by weight of brown rice powder based on 100 parts by weight of goat milk, and if less than or exceeding the above range, human immunity enhancement and physiological activity of Cordyceps sinensis It can lead to imbalance of material distribution.

In addition, in the medium composition of the present invention, in addition to the brown rice, corn rich in carbohydrates (Maize) is used, and the corn contains a large amount of unsaturated fatty acids that are good for the body in addition to carbohydrates, and the corn seeds are rich in fat. That's why you can squeeze corn oil and use it.

In particular, since the fat in corn seeds is rich in high-quality unsaturated fatty acids, especially vitamin E (tocopherol), it acts as a physiologically active substance to prevent human aging, so in the present invention, a large amount of Militaris cordyceps cultivated using corn powder in the medium composition It should contain vitamin E (tocopherol), but the corn powder is preferably used in an amount of 10 to 15 parts by weight based on 100 parts by weight of goat milk.

Also, pupae, especially silkworm pupae, are very nutritious foods. The nutritional components contained in the pupae consist of more than half of the moisture and about 20% of the protein, and more than 10% of the fat and the rest consist of ash, calcium, and phosphorus. Furthermore, essential amino acids are evenly distributed in this protein, and because the fat is composed of oleic acid and linoleic acid, it is well digested and absorbed.

If the pupae as a powder as described above is used in the medium composition of the present invention, cordyceps sinensis can be obtained, which is rich in high-quality protein, particularly essential amino acids, and the amount of pupae powder used is 10-15 parts per 100 parts by weight of goat milk. It is preferable to use it in parts by weight.

On the other hand, Spirulina is the oldest algae on Earth, and is a multicellular organism with a thin cell wall. Spirulina was used in ancient Africa and Mexico, but only recently was introduced to the modern industrial society.

The color of the spirulina is emitted by phycocyanin (blue) of chlorophyll (green) in cells, and the blue-green algae is also called blue-green algae. The scientific name of Spirulina was given by Turpin, a German ornithologist in 1827. Spirulina means spiral in Latin. It grows mainly in salt lakes in tropical regions, and it is not distributed worldwide, but the optimum water temperature is 32~42℃, and it breeds in a strong alkaline environment. Artificial farming is possible, so it can be mass-produced.

For reference, an article on Spirulina published in the magazine New Statesman (November 1967) published in London, England is as follows. “At Fort Miller in the Outback Chad Republic of the Sahara Desert, they sell black snacks called'Die'. These dumpling-shaped cookies are made from algae. African women take the algae, the raw material of these sweets, from the surface of the lake with dense baskets. This algae, called Spirulina, has a spiral shape and is common in salt lakes in Africa. On November 10, 1967, the International Society for Applied Microbiology was held in Addis Ababa, the capital of Ethiopia, and Spirulina was a hot topic. Sweden's Dr. Heddon, chairman of the international conference, said Spirulina would attract attention as a food for the future as it is rich in protein.”

As mentioned above, population growth and food shortages are emerging as important issues in today's planet. Accordingly, the United Nations (UN) is making serious efforts to secure food. Spirulina has been highly regarded as a food source by reports on Spirulina.

The main components contained in the spirulina include proteins, carbohydrates, water-soluble dietary fiber, antioxidant pigment components (chlorophyll, carotenoids, phycocyanine), antioxidant enzymes (SOD), gammarinolenic acid (GLA), and the like. Vitamins include beta-carotene, vitamins B1 · B2 · B6 · B12, vitamin E, inositol, and folic acid. It also contains minerals such as calcium, iron, potassium, magnesium, zinc, manganese, selenium and germanium.

In particular, Spirulina is a high protein food that contains more protein (69.5%) than chlorella (50%), which is famous for having a lot of protein, and contains all essential amino acids in a good balance. It is easy to see how much protein Spirulina contains compared to 39% of soybeans (soybeans), 20% of beef, and 12% of eggs. In addition, Spirulina is multicellular and has a high digestibility and absorption rate (95.1%) because it does not have a cell wall, but chlorella is a single cell and has a low digestibility (73.6%) due to its thick cell membrane.

As the pigment component of Spirulina, it contains yellow carotenoids, green chlorophyll, and blue phycocyanin. Phycocyanin is a bile pigment contained in Spirulina, which is not present in chlorella, and like animal bile pigments, it helps digest fat and exhibits anti-inflammatory and antioxidant properties. It also contains a large amount of antioxidants such as tocopherol and beta-carotene. Chlorophyll (chlorophyll) is what makes plants green and is called'green blood'. Since it contains large amounts of chlorophyll and beta-carotene, which are characteristic of green and yellow vegetables, 4 g of spirulina powder is known to be able to replace more than 40 g of green and yellow vegetables.

In addition, Dr. Takeuchi of Japan announced a case in 1980 that the administration of spirulina along with dietary therapy to diabetic patients achieved good results. Fat is not burned well in the body of diabetic patients, so there is a limit to using fat as an energy source. Glucose is also not an alternative because it raises blood sugar. As a result, the protein of Spirulina is believed to be a source of energy to reduce hunger in diabetics, boost energy, and help control blood sugar, and is loved by diabetic (adult-type) patients. In addition, the efficacy of Spirulina was tested for anemia, and good results were achieved. That is, as a result of administration of Spirulina, the number of red blood cells, hemoglobin, and hematocrit were improved.

In addition, the protein contained in Spirulina has an immunity-enhancing effect. The antibodies and lymphocytes of our body's immune system are composed of proteins. When a research team at Japan Women's University of Nutrition fed mice with spirulina-added diet, the amount of antibodies tended to increase. According to the results of animal and clinical trials for Spirulina, there are reports of cholesterol lowering, immunity enhancement, antioxidant function, weight loss, and the like. In addition, Spirulina is effective in improving malnutrition due to its abundant nutrients and high digestion and absorption rate. In addition, it is effective in diabetes, anemia, pancreatitis, liver disease, gastritis, gastric ulcer, cataract, alopecia, stress, etc., and is helpful in cancer prevention.

When the above spirulina is powdered and used in the medium composition of the present invention, cordyceps sinensis reflecting the nutrients and physiologically active substances of the spirulina can be obtained, in which case, the amount of spirulina powder used is 5 parts by weight of goat milk. It is preferable to use ~ 10 parts by weight.

In addition, seaweed contains alginic acid, which has a high content of iron, calcium and iodine, which promotes metabolism, making it slippery. The alginate refers to seaweed acid and is also referred to as algin and algin. It is a hydrophilic mucopolysaccharide (mucopolysaccharide, 粘質多糖) distributed in the cell wall of brown algae such as kelp. When combined with water, it forms a viscous secretion, gum. In general, it exists in the form of alginate such as sodium alginate and calcium alginate, and has a molecular formula of n with a _{long chain structure (C 6} H ₈ O _{6) and has weak acidity.}

When the above seaweed is pulverized and used in the medium composition of the present invention, cordyceps sinensis rich in iron, calcium, iodine and alginic acid contained in the seaweed can be obtained, and at this time, the amount of seaweed powder is 100 parts by weight of goat milk. It is preferable to use 5 to 10 parts by weight with respect to.

In addition, glucose _{is a monosaccharide having the molecular formula C 6} H ₁₂ O ₆ and its official name is glucose. Plants and algae can use light energy to make glucose from water and carbon dioxide, a process called photosynthesis. Glucose is the first substance used by cells to gain energy. The blood of animals circulates in the form of monosaccharides, and the excess glucose is stored in the form of a polymer. Animals store them as glycogen and plants store them as starch.

Glucose is an aldohexose most widely used in living organisms. Glucose has a lower tendency to react non-specifically with the amine group of proteins compared to other aldohexoses. Non-specific reaction of sugars to amine groups of proteins is called glycation, and the function of many proteins may be impaired or destroyed by this reaction. The low glycation rate of glucose is because glucose has a more stable cyclic structure than other aldohexoses. Most of the long-term complications of diabetes (eg blindness, kidney failure, and peripheral neuropathy) are presumed to be due to protein or lipid glycation. In contrast, a reaction in which sugar is added to a protein while regulated by an enzyme is called glycosylation, which, unlike glycation, is essential for the function of many proteins.

In particular, glucose is used as an energy source in most organisms from bacteria to humans through aerobic respiration, anaerobic respiration or fermentation. Glucose becomes the body's core energy source through breathing and provides about 3.75 kilocalories (kcal) of energy per gram. When carbohydrates (such as starch) are broken down, monosaccharides and disaccharides are produced, most of which are glucose. Glucose is oxidized in glycolysis followed by the citric acid cycle (TCA cycle) and oxidative phosphorylation, resulting in carbon dioxide and water, and energy is produced mainly in the form of ATP. The living body regulates the concentration of glucose in the blood by using various types of control functions, including the insulin response. Since the brain uses only glucose as an energy source, the availability of glucose has a great influence on psychological processes as well.

When the above-described glucose is used in the medium composition of the present invention, it acts as a growth energy source of Cordyceps sinensis and can rapidly grow the growth rate of Cordyceps sinensis at a stable level, and at this time, the amount of glucose used is 3 to 100 parts by weight of goat milk. It is preferable to use 5 parts by weight.

On the other hand, the method of cultivating Cordyceps sinensis using the cordyceps cultivation medium composition of the present invention, based on 100 parts by weight of goat milk, 10 to 15 parts by weight of brown rice powder, 10 to 15 parts by weight of corn powder, 10 to 15 parts by weight of pupa powder, A medium forming step of forming a medium comprising 5 to 10 parts by weight of spirulina powder, 5 to 10 parts by weight of seaweed powder, and 3 to 5 parts by weight of glucose; Inoculation step of inoculating the seed of Militaris Cordyceps sinensis into the medium; A first culture step of culturing for 10 to 15 days in a constant temperature and humidity culture room in a constant temperature and humidity culture room after the inoculation step for 10 to 15 days at a temperature of 25° C., a humidity of 80%, and an illuminance of 500 Lux; After the first culture step, a second culture step of lowering the temperature to 20° C. and culturing for 35 to 45 days in a culture condition of 80% humidity and 500 Lux of illuminance to form a fruiting body in a complete form; including, but In the secondary culture step, it comprises the step of uniformly spraying sodium alginate solution to induce stable growth of the fruiting body tissue by forming ^{Ca 2+ and calcium alginate of the goat milk.}

At this time, the characteristic composition is that the sodium alginate solution is uniformly sprayed in the second culture step, that is, alginic acid is known as an intercellular mucilage polysaccharide peculiar to brown algae, and the constituent sugar (構成糖) ), it has D-mannuronic acid (M) and L-gururonic acid (G), and the distribution in the molecule and M and G differs depending on the origin and ancestral part. Sodium alginate salt is water-soluble, but becomes a gel form due to the presence of divalent cations such as Ca2+ and Mg2+. From this property, it is used as a comprehensive immobilized carrier when adjusting immobilized cells, immobilized enzymes or artificial seeds. In particular, a solution in which cells are suspended in an aqueous sodium alginate solution is dropped into an aqueous calcium chloride solution to gel as calcium alginate, and the cells between the gels are enclosed and fixed to strengthen the cells.

Accordingly, when the sodium alginate solution is uniformly sprayed in the second culture step, the sodium alginate solution forms calcium alginate by ion exchange reaction with ^{Ca 2+ of the goat milk, and the thus formed calcium alginate is} Since it has the effect of strengthening the fruiting body, it contributes to the stable growth of cordyceps.

In addition, after the medium formation step, before the inoculation step, it is preferable for the growth of Cordyceps sinensis to sterilize the medium through a medium sterilization step of sterilizing the medium at 120 to 130°C and 15 psi (11 kg/cm ^{2) for 15 to 30 minutes.}

The Militaris Cordyceps sinensis cultivation method using the cultivation medium composition of Militaris Cordyceps sinensis, which is capable of stable cultivation of the present invention and contains a large amount of human immunity enhancing and physiologically active substances, is the protection scope of the present invention. It is self-evident that it belongs to.

Hereinafter, in [Example 1], a cordyceps cultivation method using the medium composition of the present invention is carried out, and in [Example 2], a cordyceps cultivation method using a medium composition according to Korean Patent Laid-Open Patent 10-2020-0048736 as a [Comparative Example] The results were compared and shown according to [Example 3].

[Cultivation of Cordyceps sinensis using the medium composition of the present invention]

For 100 g of goat milk, 15 g of brown rice powder, 15 g of corn powder, 15 g of pupa powder, 10 g of spirulina powder, 10 g of seaweed powder, and 5 g of glucose were mixed to form a medium, and after inoculation of Cordyceps militaris Cordyceps militaris. , In a constant temperature and humidity culture room, the first culture was carried out to allow the mycelium to adhere to the entire medium by culturing for 10 days under culture conditions of temperature 25°C, humidity 80%, and illuminance 500Lux.

After the first cultivation step, 50 ml of sodium alginate solution is evenly sprayed on the medium, and then the temperature is lowered to 20° C. and cultured for 35 days in a culture condition of 80% humidity and 500 Lux of illuminance to form a fruiting body in a complete form. The second culture was performed, and the Militaris cordyceps were harvested.

[Cultivation of Cordyceps sinensis using a medium composition according to Korean Patent Publication No. 10-2020-0048736]

After putting 40 g of brown rice and 40 g of water into a culture bottle at 25°C and 65% relative humidity, they were sterilized at 121°C for 30 minutes and naturally cooled to 25°C to prepare a brown rice medium. After mixing 5 g of yeast, 5 g of peptone, 15 g of sugar, and 1 kg of water at 25°C, sterilize at 121°C for 20 minutes and cool naturally at 25°C. Cordyceps militaris After inoculation, the liquid seed was prepared by incubating at 25° C. for 7 days. Subsequently, the liquid seed was mixed with a mixture of 80 g of yeast, 80 g of peptone and 120 g of sugar at 25°C, and cultured for 7 days in an incubator at 23°C and 65% relative humidity to prepare a seed mixed medium (inoculation source). . At 23° C., the seed mixture medium was added to and inoculated into a culture bottle containing the brown rice medium to prepare a brown rice medium mixture. At a relative humidity of 65%, the brown rice medium mixture at 23°C was rapidly lowered to a first temperature of 0°C at a temperature reduction rate of 5°C/min, and then a temperature reduction step (t1) of maintaining the first temperature of 0°C for 2 days was performed. . Subsequently, at a relative humidity of 65%, the brown rice medium mixture having a first temperature of 0° C. is gradually raised to a second temperature of 23° C. at a temperature increase rate of 01° C./min, and then the second temperature is maintained at 23° C. for 5 days (t2 ) Was performed. Subsequently, a maintenance step (t3) of maintaining the brown rice medium mixture at a second temperature of 23° C. at a relative humidity of 65% for 35 days at a second temperature of 23° C. was performed to incubate for a total of 42 days in the culturing step, and brown rice cordyceps Harvested

[Comparison measurement of mycelial growth rate, fruiting body formation, and cordycepine content]

The hyphae growth rate, fruiting body formation amount, and cordycepine content of the above [Example 1] and [Example 2] were measured, and the results are shown in Table 1 below. At this time, the mycelial growth rate and the amount of fruiting body formation were measured as a result of the relative comparison of [Example 1] and [Example 2], and if the relative comparison result was excellent, it was expressed as (+), and if not, it was expressed as (-). In addition, the content of cordycepine was measured using high-speed liquid chromatography (HPLC, Agilent 1100, series, USA), and according to a conventional method using a cordicepin standard material Sigma.

division Mycelial growth rate Amount of fruiting body formation Cordycepine content Example 1 (+) (+) 125.9 μg/mL Example 2 (-) (-) 95.5 μg/mL

As can be seen from the above [Table 1], cordyceps cultivated using the cultivation medium composition of Militaris Cordyceps sinensis, which is capable of stable culture of the present invention and contains a large amount of human immunity and physiologically active substances, has a mycelial growth rate It can be seen that the amount of fruiting body formed and the content of cordycepine are superior to those of using brown rice, yeast, peptone, and sugar, which are conventionally generally used medium compositions.

The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains will be able to make various modifications and variations without departing from the essential characteristics of the present invention. Accordingly, the embodiments and/or drawings disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain, and the scope of the technical idea of the present invention is not limited by these embodiments and/or drawings. . The scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (6)

delete delete delete Based on 100 parts by weight of goat milk, 10 to 15 parts by weight of brown rice powder, 10 to 15 parts by weight of corn powder, 10 to 15 parts by weight of pupa powder, 5 to 10 parts by weight of spirulina powder, 5 to 10 parts by weight of seaweed powder, 3 to 10 parts by weight of glucose A medium forming step of forming a medium comprising 5 parts by weight; A medium sterilization step of sterilizing the formed medium at 120 to 130°C, 15psi (11kg/cm ² ) for 15 to 30 minutes; Inoculation step of inoculating the seeds of Militaris Cordyceps sinensis into the sterilized medium; The first culture step of culturing for 10 to 15 days in a constant temperature and humidity culture room after the inoculation step at a temperature of 25° C., a humidity of 80%, and an illuminance of 500 Lux for 10 to 15 days so that the mycelium is settled on the entire medium; After the first culture step, a second culture step of lowering the temperature to 20° C. and culturing for 35 to 45 days in a culture condition of 80% humidity and 500 Lux of illuminance to form a fruiting body in a complete form; including, but In the secondary culture step, uniformly spraying sodium alginate solution to induce stable growth of the fruiting body tissue by forming ^{Ca 2+ and calcium alginate of the goat milk.} Cultivation method of Militaris Cordyceps sinensis containing a large amount of enhancing and physiologically active substances
delete Militaris Cordyceps sinensis, characterized in that it is cultivated according to the cultivation method of Militaris Cordyceps sinensis, which enables stable cultivation according to claim 4 and contains a large amount of human immunity and physiologically active substances.