KR20190065916A - Medium composition for cultivating vegetable worms and the method of cultivating vegetable worms - Google Patents

Abstract

The present invention relates to a medium composition for cultivating Cordyceps which comprises a base medium for Cordyceps cultivation, a peptide having an average molecular weight of 400 to 10,000 and fulvic acid in a weight ratio of 2 to 12 : 1 : 1 to 8, and a cultivation method of Cordyceps using the same. The present invention can further increase the content of active ingredients such as cordycepin even at the higher peptide content by using the fulvic acid together with the peptide in the medium for Cordyceps cultivation, thereby increasing the value of Cordyceps and greatly increasing the productivity of cordycepin.

Description

TECHNICAL FIELD The present invention relates to a medium composition for cultivation of Cordyceps mushroom and a method for cultivation of Cordyceps mushroom cultivar (Medium composition for cultivating vegetable worms)

The present invention relates to a culture composition for growing Cordyceps, and more particularly, to a culture composition for growing Cordyceps culophus supplemented with fulvic acid and a method for cultivating caterpillar fungus using the same.

Cordyceps is a kind of mushroom which is called insecticide in winter and grass like in summer. It is insecticide-producing medicinal mushroom which is a type of fungus that enters into the body of living insect. Among the Chinese caterpillar fungi, three types are recognized as Cordyceps sinensis, Codyceps militaris and Paecilomyces japonica. Two of them, which are known to be used globally, are Cordyceps sinensis and Millilitaris Cordyceps sinensis, which is a complete strain of sexual reproduction. Snowflake Cordyceps is an incomplete fungus recognized in Korea.

Cordyceps has been widely used for the treatment and health maintenance of various diseases caused by Donguibogam. In China, athletes are taking the Chinese caterpillar fungus and representing the miraculous records, thus recognizing the Chinese caterpillar fungus as the three major natural food health claims.

Cordyceps are among the highest protein content (more than 28% of dry matter) of crops and contain large amounts of substances that enhance immunity. Cordycepin (3'-deoxy-adenosine), which is an isomer of quinic acid, is included in the immune active substance of Cordyceps, and the content of cordycepin is higher than that of other Cordyceps Is much higher. Codisepin is a nucleic acid substance that acts on genetic information of cells and activates degraded immune function to prevent normal cells from being converted into cancer cells. In clinical trials in which Cordyceps was injected, the amount of NK cells (natural killer cells) and cytokines secreted from immune cells increased by 18% ~ 25%, which is an immune cell that kills cancer cells. And has received much attention as a health functional food. Based on these results, Cordycepsin - containing Cordyceps was the only mushroom in Korea to be certified as a health food. In addition, the results of clinical studies have been published that the Chinese caterpillar fungus is very helpful in controlling blood pressure and diabetes, lung function and liver function.

As such, Cordyceps is an important crop which has been continuously studied in the domestic medicinal and food fields because of its high value as a crop. Therefore, it is recognized that it is important not only as a medicine but also as a health supplement, and various powder or liquid form products are now commercially available have. In addition, studies on the development of pollution-free microbial pesticides by using the control of the density of insects in natural ecosystem have been actively carried out. The development of such natural pesticides is not limited to the control of insect pests, Which can solve the environmental pollution problem caused by the environment.

Although caterpillar fungus is consumed much among mushrooms, the quantity collected from natural is very low and can not meet the demand, so many farmers around the world make artificial cultivation methods and cultivate them in large quantities. However, it is very difficult to cultivate because it is a plant that grows slowly and is sensitive to external environment and can not spray pesticides. Although it succeeded in large-scale cultivation, it has been known that the number of cultivated caterpillar fungus is low even in a farm where it has been cultivated for a long time. In other words, when 10 plants are cultured and harvested, about 1-2 cases are contaminated or abnormal strains grow and the probability of watering is lowered.

Currently, the most commonly used methods for the cultivation of Chinese caterpillar fungus include the method of cultivating caterpillar pupa caterpillar fungus which is inoculated directly with silkworm larvae, the method of cultivating brown rice + pupa singer caterpillar fungus by inoculating the fungus in a mixture of silkworm powder and brown rice, Method is to grow the germanium components, etc., and inoculated in the sterilized state, it is common to grow on a flat plate or in a bottle like other mushrooms.

Studies on the development and cultivation of seeds to enhance the pharmacokinetic properties and efficacy of Chinese caterpillar fungus have been conducted domestically in China, Korea, and Japan. However, nowadays, to be.

On the other hand, fulvic acid (Fulvic acid) refers to a fraction which is not precipitated in an acidic (pH 1) organic matter extracted from soil organic matter by alkali. Flavic acid is a mixture of various substances such as carboxy-rich corrosive substances, polysaccharides, glycosides and phenolic substances. The color of the solution is yellow to brown and the absorbance of visible part per unit concentration is much lower than that of the acid.

Fulvic acid is adsorbed in iron, aluminum oxide, clay minerals, and polymer organic compounds in soil, and adsorbs various exchangeable cations by their dissociation groups. Therefore, these cations are closely related to the effectiveness, migration and accumulation, and buffering effects on soil environmental changes.

Korean Patent Registration No. 10-1264441 Korean Patent Registration No. 10-1421742 Korean Patent Registration No. 10-1036054

The present invention aims to provide a culture medium for a Chinese cabbage growing plant which can increase the growth rate of Chinese cabbage and increase the content of cordycepin contained in Chinese cabbage, by adding fulvic acid to a basic medium for the production of Chinese cabbage, and to provide a method for cultivating Chinese cabbage .

In order to achieve the above object, the present invention provides a culture medium for insect waxy herb cultivation, which comprises 0.1 to 20 parts by weight of fulvic acid in 1 to 10 parts by weight of a basic medium for insect growth.

In the above composition, the basic medium for growing Cordyceps horticulture is preferably at least one selected from brown rice, germinated brown rice, corn, and pupa.

The composition preferably further comprises 0.1 to 10 parts by weight of a peptide having an average molecular weight of 400 to 10,000. It is more preferable that the peptide is derived from collagen. It is more preferable that the peptide is an oligopeptide having an average molecular weight of 400 to 800.

In the composition, the fulvic acid is preferably in the form of a powder or a solution.

The present invention also provides a method for cultivating a Chinese caterpillar fungus, comprising the step of inoculating and cultivating a Chinese cabbage to a culture medium containing 1 to 10 parts by weight of a basic medium for insect growth, and 0.1 to 20 parts by weight of fulvic acid.

In the above method, it is preferable that the basic medium for growing Cordyceps horticulture is at least one selected from brown rice, germinated brown rice, corn, and pupa.

The culture medium for insect waxy cultivation of the present invention can increase the growth rate of Cordyceps sinensis and increase the content of active ingredient such as cordisepin by mixing fulvic acid with the basic medium for insect hatchery cultivation. The present invention solves the conventional problem that the content of the active ingredient such as cordycepin did not increase when the level of the active ingredient such as cordycepsin was increased to a large extent, and the content of cordycepin in the cordyceps was greatly increased And the productivity of codisepine can be greatly increased.

The culture medium for cultivation of Cordyceps mushroom according to the present invention comprises a basic medium for the growth of Cordyceps, and fulvic acid.

In the present invention, the "basic medium for cultivation of Cordyceps," is defined as a medium capable of supplying at least one of carbohydrates, proteins, sugars, minerals and water as a solid or liquid medium which can be used for cultivation of Cordyceps.

Preferably, one or more of brown rice, germinated brown rice, corn, pupa, etc. may be used as the basic medium for the insect growth. The pupa is not particularly limited and can be used for the insect pupa of general insects. More preferably, a pupa of crickets or the like can be used. The brown rice, germinated brown rice, corn, pupa, etc. are preferably used in powder form.

The basic medium for growing Cordyceps mushroom may further contain water if necessary.

In the culture medium for insect growth of the present invention, fulvic acid promotes the growth of Cordyceps sinensis to increase the content of cordisepin.

Flavic acid can be used in the form of a powder or solution. When used in the form of a solution, it is preferable to use the solution in a concentration of 1 to 5%. It is preferred to use water, especially sterile purified water, as the solvent.

The fructic acid is preferably used in an amount of 0.1 to 20 parts by weight per 1 to 10 parts by weight of the basic medium.

In nature, caterpillar fungus grows with mediators of high protein content, such as insect larvae, pupae, or insects. The size of the polypeptide chain constituting the protein of insects and larvae is smaller than that of a general animal polypeptide, and there are many types of peptides which are easily absorbed. In order to grow caterpillar fungus, insects and caterpillars are infected with caterpillar fungus, and grow on the ground or grow on trees. The insects and larvae, which are the medium of insects and larvae, are decomposed by various conditions such as humidity, In the process, caterpillar fungus grows. Thus, the polypeptides of insects and larvae are also degraded and become a nutrient source for the growth of Cordyceps. Therefore, by decomposing and feeding the high-protein nutritional polypeptide into a peptide that is easily absorbed, conditions similar to those at the time when the Chinese caterpillar fungus grows in nature can be formed.

The culture medium for insect growth of the present invention may further comprise 0.1 to 10 parts by weight of a peptide having an average molecular weight of 400 to 10,000 with respect to 1 to 10 parts by weight of the basic medium.

As the peptide, it is preferable to use a peptide having an average molecular weight of 400 to 10,000, and it is more preferable to use an oligopeptide having an average molecular weight of 400 to 800.

Various kinds of proteins such as insects, larvae, and collagen can be used as proteins for peptide production. Among them, collagen is preferable to use collagen as a raw material for producing peptides because it is easy to adjust the molecular weight constantly, supplies materials smoothly, is economical, and has excellent products. Representative examples of collagen include fish collagen (collagen obtained from fish scales), donkey collagen (refined collagen obtained from pig skin), and Uffi collagen (refined collagen obtained from cowhide).

Cultivation of Cordyceps sinensis using the culture composition of the present invention as described above has the effect of increasing the growth rate of Cordyceps sinensis and increasing the content of cordycepin contained in Cordyceps sinensis.

A method for growing Cordyceps sinensis using the above-mentioned culture composition of the present invention is as follows.

First, cordyceps are inoculated and cultured in the medium composition of the present invention. The culture is preferably carried out in an incubation room maintained at 15 to 28 캜, more preferably at 19 to 23 캜.

Growth speed of snowflake Cordyceps is faster than that of Millitarian Cordyceps, and its harvesting period is short. The harvest period can vary depending on the season, temperature, light dose, humidity, etc., depending on the conditions of the season or grower. However, it is generally possible to harvest the seedlings from 45 to 60 days after inoculation.

Millitarian Cordyceps are slow growing and have a long harvesting period compared to those of snowy caterpillars. The harvest period can vary depending on the season, temperature, light dose, humidity, etc., depending on the conditions of the season or grower, but generally, the Millitarian Cordyceps can be harvested within 75-80 days after inoculation.

[ Example ]

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. The following examples and experimental examples illustrate the present invention and are not intended to limit the scope of the present invention.

< Example  1>

3 g of the puddle pupa, 5 g of the peptide and 5 g of fulvic acid were mixed to prepare a culture composition for growing Cordyceps. At this time, fulvic acid was used in the form of an aqueous solution having a concentration of 1%, and the peptide was a water soluble collagen low molecular peptide having an average molecular weight of 600 to 800.

< Example  2>

10 g of pupa and 5 g of fulvic acid were mixed to prepare a culture composition for growing Cordyceps. Fulvic acid was used in the form of a 1% aqueous solution.

< Example  3>

3 g of pupa and 35 g of fulvic acid were mixed to prepare a culture composition for growing Cordyceps. Fulvic acid was used in the form of a 1% aqueous solution.

< Example  4>

5 g of pupa and 35 g of fulvic acid were mixed to prepare a culture composition for growing Cordyceps. Fulvic acid was used in the form of a 1% aqueous solution.

< Example  5>

250 g of corn and 25 g of fulvic acid were mixed to prepare a culture composition for growing Cordyceps. Fulvic acid was used in the form of a 1% aqueous solution.

< Example  6>

250 g of corn and 5 g of fulvic acid were mixed to prepare a culture composition for growing Cordyceps. Fulvic acid was used in the form of a 1% aqueous solution.

< Example  7>

50 g of brown rice, 30 g of water, 5 g of peptide, and 35 g of fulvic acid were mixed to prepare a culture medium for insect waxy cabbage cultivation. At this time, fulvic acid was used in the form of an aqueous solution having a concentration of 1%, and the peptide was a water soluble collagen low molecular peptide having an average molecular weight of 600 to 800.

< Example  8>

40 g of brown rice, 10 g of pupa and 40 g of fulvic acid were mixed in Dongae and the like to prepare a culture composition for growing Cordyceps. Fulvic acid was used in the form of a 1% aqueous solution.

< Experimental Example  1>

The content of cordycepin according to the composition of the medium

Using the culture compositions prepared in Examples 1 to 6, the content of cordycepin according to the composition of the culture medium was confirmed as follows.

As the seed germ, Doyong reed germ seed was used, and seed culture was inoculated into the sterilized medium composition.

100 seeds were inoculated into a sterilization culture vessel and cultured in a culture room at 19 to 23 ° C.

The content of cord seeds of Cordyceps mushroom cultivated as described above was analyzed as follows.

Ten mycelia and fruiting bodies were taken from the caterpillar fungus cultivated for 60 days after inoculation, and lyophilized. Samples of (1) and (2) above were analyzed for mycelium and fruiting bodies, respectively, and only fruiting bodies for the remaining samples were analyzed.

10 g of mycelial or fruiting body of dried Cordyceps sinensis was dispersed in 190 g of purified water, extracted at 80 ° C for 2 hours, centrifuged, and the supernatant was filtered to use as an analytical sample. The content of codistin was analyzed by HPLC, and the reference material of codistepin was purchased from Sigma Aldrich.

The analysis was commissioned by the Korea Research Institute of Chemical Technology. The composition of the medium and the resulting content of cordycepin were analyzed and the results are shown in Table 2 below.

Analyzed: Composition of medium Codispein content (mg / kg) Mycelium: Example 1 1316 Fruiting body: Example 1 1095 Mycelium: Example 2 654 Fruiting body: Example 2 835 Fruiting body: Example 3 474 Fruiting body: Example 4 1936 Fruit body: Example 5 723 Fruiting body: Example 6 457

As shown in the results of Table 2, in all of the culture compositions to which fulvic acid was added, the content of cordisepin was high in both mycelium and fruiting body, and fruiting bodies using the culture composition of Example 4 in which 5 g of chrysalis and 35 g of fulvic acid were mixed Codispine content was the highest.

Claims (8)

Wherein the composition comprises 0.1 to 20 parts by weight of fulvic acid in 1 to 10 parts by weight of a basic medium for the production of Cordyceps mushroom cultivation. The method according to claim 1,
Wherein the basic medium for the production of Cordyceps mushroom is at least one selected from brown rice, germinated brown rice, corn, and pupa. The method according to claim 1,
Wherein the composition further comprises 0.1 to 10 parts by weight of a peptide having an average molecular weight of 400 to 10,000. The method of claim 3,
Wherein the peptide is derived from collagen. 5. The method of claim 4,
Wherein the peptide is an oligopeptide having an average molecular weight of 400-800. 6. The method according to any one of claims 1 to 5,
Wherein the fructic acid is in the form of a powder or a solution. A method for cultivating a Chinese caterpillar fungus, comprising the step of inoculating and cultivating a Chinese caterpillar fungus in a culture medium containing 1 to 10 parts by weight of a basic medium for the production of Chinese cabbage seeds and 0.1 to 20 parts by weight of fulvic acid. 8. The method of claim 7,
Wherein the basic medium for the production of Cordyceps mushroom is at least one selected from brown rice, germinated brown rice, corn, and pupa.