KR102550055B1 - Grains cultured with persimmons, tomatoes and edible insects, and method for manufacturing the same - Google Patents

Abstract

The present invention provides cereals characterized in that they are inoculated and cultured using a composition comprising persimmon, tomato and edible insects.

Description

Grains cultured with persimmons, tomatoes and edible insects, and method for manufacturing the same}

The present invention relates to grains cultured with persimmons, tomatoes, and edible insects and a method for producing the same, and more particularly, to grains inoculated and cultured using a composition containing persimmons, tomatoes, and edible insects, and a method for producing such grains. will be.

Mushrooms (fungi, 茸類) is a general term for higher fungi belonging to the basidiomycetes. These mushrooms are classified into basidiomycetes and ascomycetes according to the generation method of spores made for breeding, and most edible mushrooms belong to the basidiomycetes. There are thousands of known types of mushrooms, but about 180 species that are currently edible are known.

The general components of these mushrooms are similar to vegetables, and are characterized by a high content of ergosterol, a provitamin D2. However, because mushrooms have many enzymes, they are easy to deteriorate. Therefore, it is common to store it after drying or salting. Mushrooms are highly palatable foods with a unique flavor (aroma, taste). In addition, shiitake mushrooms and button mushrooms contain ingredients with special physiological effects.

On the other hand, cordycepin can inhibit protein kinase activity, inhibit the cracking of glycosyl nucleoside phosphorylase to regulate humoral immunity, and inhibit the production of NO products, thereby suppressing inflammation. In addition, cordycepin exhibits excellent physiologically active functions such as cancer cell suppression function and leukemia suppression function.

In order to include or increase the cordycepin content in mushrooms, a culture medium is required.

Patent Document 1: Korean Registered Patent No. 10-2287039

The present invention has been made to solve the above problems, and an object of the present invention is to provide inoculated and cultured grains using a composition containing persimmons, tomatoes and edible insects, and a method for producing the same.

The object of the present invention is not limited to the tasks mentioned above, and other tasks not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the above object, the present invention

It provides cereals characterized in that they are inoculated and cultured using a composition containing persimmons, tomatoes and edible insects.

In addition, the composition includes persimmon extract extracted from persimmon, ground product obtained by grinding tomatoes, ground product obtained by grinding crickets and mealworms as edible insects, and water, and the persimmon extract is obtained by drying persimmon with hot air to obtain dried persimmon powder, Here, water was mixed at a rate of 50-100 ml per 1 g of powder, extracted with pressurized hot water at a pressure of 1-2 MPa and a temperature of 95-105 ° C, and the extract obtained by pressurized hot water was filtered, reduced pressure, concentrated, and frozen. It is obtained by drying, and the composition contains 18-22 parts by weight of persimmon extract, 8-12 parts by weight of crushed tomatoes, and 8-12 parts by weight of crushed crickets and mealworms as edible insects, based on 100 parts by weight of water. It is characterized by including.

Furthermore, the present invention

Sealing the grain after putting it into a container;

Inoculating the grains in the container with a composition containing persimmons, tomatoes, and edible insects;

agitating the container; and

It provides a grain manufacturing method comprising the step of culturing the agitated container.

In addition, the inoculating step may include locating the sealed container using a collaborative robot, checking the inoculation point of the sealed container, inoculating the composition at the inoculation point of the sealed container, and forming the inoculation point at the inoculation point. and sealing the inoculum.

In addition, the stirring process is characterized in that the sealed container is shaken and stirred for 1-2 minutes using a collaborative robot.

The cereals according to the present invention are inoculated and cultured using persimmons, tomatoes, and edible insects, and when mushrooms are cultivated using them as a medium, the content of the functional component (cordycepin) of the mushrooms can be increased.

1 is a flow chart showing a method for producing cereals provided in one aspect of the present invention;
Figure 2 is a photograph showing cereals sealed in bags according to an embodiment of the present invention;
3 is a photograph showing homogenization of a composition with a homogenizer according to an embodiment of the present invention;
4 is a photograph showing the inoculation of a composition according to an embodiment of the present invention;
5 is a photograph showing sealing with silicone after inoculation according to an embodiment of the present invention;
6 is a photograph showing a wire culture die of a culture device in which culture is performed according to an embodiment of the present invention;
7 is a photograph showing grains cultured (left side of the photo) and grains that have not been cultured (right side of the photo) according to an embodiment of the present invention.

Hereinafter, various embodiments will be described in more detail with reference to the accompanying drawings. The embodiments described in this specification may be modified in various ways. Certain embodiments may be depicted in the drawings and described in detail in the detailed description. However, specific embodiments disclosed in the accompanying drawings are only intended to facilitate understanding of various embodiments. Therefore, the technical idea is not limited by the specific embodiments disclosed in the accompanying drawings, and it should be understood to include all equivalents or substitutes included in the spirit and technical scope of the invention.

Terms including ordinal numbers such as primary, secondary, first, and second may be used to describe various elements, but these elements are not limited by the above-mentioned terms. The terminology described above is only used for the purpose of distinguishing one component from another.

In this specification, terms such as 'comprise' or 'having' are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded. It is understood that when a component is referred to as being 'connected' or 'connected' to another component, it may be directly connected or connected to the other component, but other components may exist in the middle. It should be. On the other hand, when a component is referred to as being 'directly connected' or 'directly connected' to another component, it should be understood that no other component exists in the middle.

In addition, in describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be abbreviated or omitted.

The present invention provides cereals characterized in that they are inoculated and cultured using a composition comprising persimmon, tomato and edible insects.

The cereals according to the present invention are inoculated and cultured using persimmons, tomatoes, and edible insects, and when mushrooms are cultivated using them as a medium, the content of the functional component (cordycepin) of the mushrooms can be increased.

Hereinafter, cereals according to the present invention will be described in detail.

The grain may be brown rice, rice, etc., preferably brown rice.

The composition preferably includes a persimmon extract extracted from persimmon, ground water obtained by grinding tomatoes, ground water obtained by grinding crickets and mealworms as edible insects, and water.

In addition, the persimmon extract is dried with hot air to obtain dried persimmon powder, mixed with water at a rate of 50-100 ml per 1 g of powder, and pressurized at a pressure of 1-2 MPa and a temperature of 95-105 ° C. It may be obtained by extracting with hot water, filtering the extract obtained with pressurized hot water, concentrating under reduced pressure, and freeze-drying.

In addition, the composition comprises 18-22 parts by weight of persimmon extract, 8-12 parts by weight of crushed tomato, and 8-12 parts by weight of crushed crickets and mealworms as edible insects, based on 100 parts by weight of water. Preferably, the composition is obtained by mixing 19-21 parts by weight of persimmon extract, 9-11 parts by weight of crushed tomato, and crickets and mealworms as edible insects at a weight ratio of 1:2 with respect to 100 parts by weight of water. It is preferable to include 9-11 parts by weight of the ground material.

In addition, the present invention

Sealing the grain after putting it into a container;

Inoculating the grains in the container with a composition containing persimmons, tomatoes, and edible insects;

agitating the container; and

It provides a grain manufacturing method comprising the step of culturing the agitated vessel.

At this time, the grain manufacturing method provided in one aspect of the present invention in Figure 1 is shown in a flow chart, hereinafter, with reference to the flowchart of Figure 1, the grain manufacturing method according to the present invention will be described in detail for each step.

First, the grain manufacturing method according to the present invention includes the step of sealing the grain after putting it into a container.

The grains may be brown rice, rice, etc., and brown rice is preferably used.

The grains may be washed, immersed in water, and then subjected to a pretreatment process of removing water.

The immersion may be performed in water, preferably in water to which glycation softening glucose is added. In addition, the immersion is preferably performed for 10-12 hours.

It is preferable to use a plastic bag for sterilization including a double-sided filter for the container. In Figure 1, grains sealed in a plastic bag for sterilization containing a double-sided filter are shown in pictures.

It is preferable to first sterilize the container at a temperature of 100-110 ° C and second sterilize at a temperature of 115-125 ° C before the inoculation step. After the secondary sterilization, cooling and inoculation may be performed.

As a specific example, the grain manufacturing method includes the steps of washing grains and immersing them in water to which glycated glucose is added for 10-12 hours; Obtaining grains immersed in water to which glycation softening glucose is added and removing water; Putting the drained grains into a sterilization plastic bag containing a double-sided filter and then sealing it; Primary sterilization of the sterilization plastic bag containing the grains at a temperature of 100-110 ° C; and secondary sterilization of the primary sterilization plastic bag at a temperature of 115-125°C.

Next, the method for producing grains according to the present invention includes inoculating the grains in the container with a composition containing persimmons, tomatoes, and edible insects.

The composition for inoculating the cereals preferably includes persimmons, tomatoes, and edible insects.

The persimmons include general raw persimmons, sweet persimmons, daebung, dried persimmons, bansi, yeonsi, persimmons, and the like, and it is preferable to use crickets and mealworms as the edible insects.

The persimmons may be ground persimmons, persimmon extracts extracted from persimmons, and persimmon extracts are preferably used.

The persimmon extract is obtained by drying persimmon hot air to obtain dried persimmon powder, mixing water therein at a rate of 50-100 ml per 1 g of powder, and pressurized hot water extraction at a pressure of 1-2 MPa and a temperature of 95-105 ° C. And, it may be obtained by filtering the extract obtained by pressurized hot water, concentrating under reduced pressure, and freeze-drying.

The tomato is preferably used as a ground tomato.

As the edible insect, it is preferable to use ground edible insects.

The composition for inoculating cereals may include the persimmon extract, ground product obtained by grinding tomatoes, ground product obtained by grinding crickets and mealworms as edible insects, and water, and the composition for inoculating cereals may include water based on 100 parts by weight of water. It is preferable to include 18-22 parts by weight of persimmon extract, 8-12 parts by weight of crushed tomato, and 8-12 parts by weight of crushed crickets and mealworms as edible insects.

The composition to inoculate the grain is preferably mixed with the composition and homogenized using a homogenizer (see FIG. 3).

In addition, the step of inoculating is preferably performed using a syringe-type dispenser. (See Fig. 4)

The syringe-type dispenser has two outlets at the end of the syringe needle, and allows the composition to be evenly sprayed on the grain surface.

After performing the inoculation step, a step of sealing the inoculation hole formed due to the inoculation may be further included. The sealing may be performed by silicone sealing using a glue gun. (See Fig. 5)

As a specific example, in the method for producing cereals, dried persimmon powder is obtained by hot air drying persimmon with respect to 100 parts by weight of water, mixing water therein at a rate of 50-100 ml per 1 g of powder, and pressure of 1-2 MPa And pressurized hot water extraction at a temperature of 95-105 ° C, 18-22 parts by weight of persimmon extract obtained by lyophilizing the extract obtained by pressurized hot water after filtration under reduced pressure and concentration, 8-12 parts by weight of crushed tomatoes and food Prepare a composition containing 8-12 parts by weight of ground water by mixing crickets and mealworms at a weight ratio of 1:2 as insects, and using a syringe-type dispenser with two outlets at the end of a syringe needle for the secondary sterilization. Inoculating the grains in the treated plastic bag for sterilization; may include.

Next, the grain manufacturing method according to the present invention includes the step of stirring the container.

In the above step, the container is subjected to agitation, and may be agitated for 1-2 minutes.

Next, the grain manufacturing method according to the present invention includes culturing the agitated container.

The culturing is performed for 30-60 days at a temperature of 17-25 ° C. in a culture device, the culture device includes a wire culture die, and the inner wall is formed of a silver foil reflector. The wire culture die has a mesh structure through wires, and by using the wire culture die, air circulation can be smoothly and evenly cultured. In addition, by forming the inner wall as a silver foil reflector, radiant heat is evenly distributed so that cultivation is more easily performed.

As a specific example, in the grain manufacturing method, the agitated sterilization plastic bag is cultured for 30-60 days at a temperature of 17-25 ° C. in a wire culture die of a culture device, but the culture device is a wire culture die (see FIG. 6) ), and the inner wall of the culture device is characterized in that it includes a silver foil reflector.

In addition, the method for producing cereals according to the present invention can be performed by automating the inoculation line. The inoculation line includes the steps of inoculating, stirring, and culturing in the method for producing cereals, and the inoculating step involves positioning the sealed container using a collaborative robot, and Confirming the inoculation point, inoculating the composition at the inoculation point of the sealed container, and sealing the inoculation hole formed at the inoculation point. In addition, the agitating process includes shaking the sealed container for 1-2 minutes using a collaborative robot for agitation process. Furthermore, the culturing step includes moving the agitated container to a culture device using a collaborative robot and culturing the container. In this way, mass production is possible by automating the inoculation line.

Hereinafter, the present invention will be described in more detail through examples.

These examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited to these examples.

<Example 1> Preparation of cereals

After washing the brown rice, it was soaked for 12 hours in water to which glycated glucose was added. Afterwards, it was put on a sieve to remove excess moisture to prepare grains.

After putting the prepared grains in a sterilization plastic bag containing a double-sided filter, it was sealed by performing a sealing treatment three times. After sealing, the first sterilization treatment was performed at a temperature of 105 ° C, and then the second sterilization treatment was performed at a temperature of 121 ° C, followed by cooling.

Raw persimmon was dried with hot air to obtain dried persimmon powder, mixed with water at a rate of 80 ml per 1 g of powder, extracted with pressurized hot water at a pressure of 1 MPa and a temperature of 100 ° C, and filtered the extract obtained by pressurized hot water. Then, it was concentrated under reduced pressure and lyophilized to prepare a persimmon extract. Tomatoes were ground using a grinding machine to prepare ground water, and crickets and mealworms as edible insects were mixed in a weight ratio of 1: 2, and then ground water was prepared by grinding using a grinding machine. A composition was prepared by mixing 20 parts by weight, 10 parts by weight, and 10 parts by weight of the prepared persimmon extract, crushed tomato, and ground edible insects, respectively, with respect to 100 parts by weight of water, and homogenized using a homogenizer. After the prepared composition was put into a composition container connected to a syringe-type dispenser and inoculated into a bag containing cereals, the hole (inoculation port) formed by the inoculation was sealed using a glue gun. Then, the bag was shaken and stirred for 1 minute.

Thereafter, grains were prepared by placing the bag containing the grains on a die of a culture device including a wire culture die and having a silver foil reflector on the inner wall and culturing at a temperature of 17-25 ° C. for 30-60 days.

<Example 2> Cultivation of Cordyceps sinensis

Cordyceps fungus was cultured using the cereals prepared in Example 1. Specifically, after inoculating Cordyceps fungus using the cereal prepared in Example 1 as a medium, it was cultured at 25 ° C. for 7 days.

<Comparative Example 1>

Cordyceps was purchased on the market.

<Comparative Example 2>

Cordyceps sinensis, which is on the market, was purchased.

<Experimental Example 1> Inorganic substance content analysis

Inorganic content of cereals and cordyceps rice prepared in Example 1 and Comparative Example 1 was analyzed. It was analyzed by requesting the Korea Functional Food Research Institute (KHSI), and the results are shown in Table 1 below.

Test Items Comparative Example 1 Example 1 vitamin e
0.57mg/100g 1.34mg/100g calcium 8.93mg/100g 12.17mg/100g potassium 250.86mg/100g 300.31mg/100g person 350mg/100g 386.99mg/100g steel 0.89mg/100g 0.99mg/100g zinc 2.21mg/100g 3.58mg/100g

As shown in Table 1, it can be seen that the grains according to the present invention have a significantly high content of inorganic substances.

<Experimental Example 2> Cordycepin content analysis

Cordycepin content of Cordyceps sinensis prepared in Example 2 and Comparative Example 2 was analyzed. It was analyzed by requesting the Korea Institute of Science and Technology (KIST), and the results are shown in Table 2 below.

Test Items Test result Example 2 Cordycepin 977.96 ppm Comparative Example 1 125.10 ppm

As shown in Table 2, it was confirmed that cordycepin content was significantly high in Cordyceps sinensis prepared by applying the cereals according to the present invention as a medium.

Claims (5)

Persimmons were dried with hot air to prepare dried persimmon powder, mixed with water at a rate of 80 ml per 1 g of persimmon powder, extracted with pressurized hot water at a pressure of 1 MPa and a temperature of 100 ° C, and filtered the extract obtained by pressurized hot water. Preparing a persimmon extract by freeze-drying under reduced pressure and concentrating;
Grinding tomatoes with a crusher to prepare crushed tomatoes;
Mixing crickets and mealworms as edible insects in a weight ratio of 1:2, and then grinding them with a grinder to prepare ground edible insects;
Washing brown rice and soaking it in glucose-added water for 12 hours;
Obtaining brown rice immersed in water to which glucose is added and removing water;
Putting the drained brown rice into a plastic bag for sterilization including a double-sided filter and then sealing it;
Primary sterilization of the sterilization plastic bag containing the brown rice at a temperature of 105 ° C;
Secondary sterilization of the primary sterilization plastic bag at a temperature of 121 ° C;
preparing a composition by mixing 20 parts by weight of the persimmon extract, 10 parts by weight of the ground tomato and 10 parts by weight of the ground edible insect material with respect to 100 parts by weight of water and homogenizing using a homogenizer;
Inject the prepared composition into the secondary sterilized plastic bag for sterilization using a syringe-type dispenser having two outlets at the end of the syringe needle, and position the secondary sterilized plastic bag for sterilization using a collaborative robot. Step, characterized in that for confirming the point for injection into the plastic bag for secondary sterilization treatment, and injecting the composition at the point for injection into the plastic bag for secondary sterilization treatment;
Sealing the hole formed by the injection with silicon using a glue gun;
Shaking and agitating the silicone-sealed plastic bag for sterilization using a cooperative robot for 1 minute; and
Preparing brown rice including; and
A method of culturing Cordyceps sinensis comprising the step of inoculating Cordyceps fungi using the brown rice as a medium and then culturing at 25 ° C. for 7 days, wherein the cordycepin content of Cordyceps sinensis is 977.96 ppm. delete delete delete delete

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