KR20210142322A - Method for cultivation of shiitake mushroom and, shiitake mushroom produced by the process - Google Patents

Abstract

The present invention relates to a method for cultivating Lentinus edodes and Lentinus edodes cultivated thereby. An objective of the present invention is to provide a method for cultivating Lentinus edodes, which forms a moisturizing film on a sawdust medium so that moisture management for the sawdust medium is performed easily even after a moisture bag of the sawdust medium is removed, thereby realizing stable growth of Lentinus edodes, and Lentinus edodes cultivated thereby. According to the present invention, a method for cultivating Lentinus edodes using a sawdust medium comprises: a stocking step of stocking a cultured sawdust medium completing inoculation and cultivation of a mushroom spawn in a cultivation building; a bag removal step of removing a moisture bag of the sawdust medium stocked in the cultivation building to expose the sawdust medium to the outside; a coating step of coating the sawdust medium with a moisturizing coating solution to form a moisturizing film; and a growth step of culturing the sawdust medium on which the moisturizing film is formed to grow Lentinus edodes. Accordingly, moisture management is performed by the moisturizing film.

Description

A method for cultivation of shiitake mushrooms and shiitake mushrooms grown thereby

The present invention relates to a method for cultivating shiitake mushrooms and to shiitake mushrooms grown by the method, wherein a moisturizing film having moisturizing and antibacterial properties is generated on sawdust medium, so that moisture management and growth can be improved after removal of the moisturizing bag until the time of shiitake mushroom cultivation It relates to a method for cultivating shiitake mushrooms that can be easily made and to shiitake mushrooms grown thereby.

Shiitake mushroom (Lentinula edodes) is a nutritious food that contains high-quality protein, fat, and various vitamins and minerals and has a unique flavor. ), chestnut, hornbeam, etc., grow on dry trees of broad-leaved trees.

Such shiitake mushrooms are cultivated by artificially culturing shiitake mushrooms and then inoculating them on logs, but this log cultivation method leads to a shortage of hardwood resources, and it is difficult to secure large quantities of logs when necessary. .

In the prior art, in consideration of the problems of the log cultivation method as described above, a sawdust medium mixed with a plurality of materials is put in a bottle or bag and sterilized, and then the shiitake mushroom spawn is inoculated and the bag is cut or removed when fruiting bodies occur. There was a problem in that moisture management suitable for mushroom growth was difficult because the moisture of the sawdust medium was dried due to incision or removal of the bag.

Laid-Open Patent Publication No. 10-2019-0040536 (2019.04.19) Laid-open Patent Publication No. 10-2014-0021857 (2014.02.21) Registered Patent Publication No. 10-1874370 (2018.06.28) Registered Patent Publication No. 10-1509470 (2015.04.01)

It is an object of the present invention to form a moisturizing film having moisturizing properties and antibacterial properties on the sawdust medium, so that even after removing the moisture bag of the sawdust medium, moisture management for the whole rice medium is made easy, and the growth of shiitake can be made stably. To provide a shiitake mushroom cultivation method and the shiitake mushroom grown thereby.

Another object of the present invention is to provide a method for cultivating shiitake mushrooms in which the moisture film produced in the sawdust medium is biodegradable, so that the treatment of the sawdust medium after cultivating shiitake can be easily performed.

The present invention provides a method for cultivating shiitake mushrooms using sawdust medium; Stocking step in which the cultured sawdust medium is stocked into the grower after the mushroom spawn is inoculated; Bag removal step in which the moisture bag of the sawdust medium stocked with the grower is removed and the sawdust medium is exposed to the outside; A coating step in which a moisture film is formed on the sawdust medium; Including, the moisture management is made by the moisture film; including the growth stage in which the shiitake mushroom is grown by culturing the sawdust medium in which the moisture film is formed.

In the present invention, the moisturizing film is made of a moisturizing coating solution, wherein the moisturizing coating solution consists of 100 parts by weight of a coating stock solution and 200 to 500 parts by weight of water, and the coating solution consists of 100 parts by weight of an eco-friendly coating solution and 3 to 10 parts by weight of ginkgo extract, , The eco-friendly coating solution contains corrosive organic matter 5-20 wt%, collagen 8-18 wt%, lignin 5-20 wt%, moisture absorbent 5-15 wt%, surfactant 2-10 wt%, stabilizer 3- 15wt%, film-former 2~10wt%, water 20~50wt%, so that the moisture film is easily formed on the sawdust medium.

According to the present invention, the sawdust medium is provided with a moisturizing film that suppresses moisture evaporation, and the moisture retention of the sawdust medium is improved. There is an effect that moisture management is made easily.

In particular, in the present invention, since the moisturizing film contains humic organic matter, collagen and lignin, it not only biodegrades after a predetermined time has elapsed, but also allows moisture control by a moisture absorbent, providing moisture necessary for the growth of shiitake mushrooms. This has an effect.

In addition, the present invention has the effect of growing shiitake mushrooms containing organic acids and mineral components contained in the ginkgo extract, as well as providing the control and antibacterial effect by the ginkgo extract.

The present invention has the effect that the moisture film produced in the sawdust medium has biodegradability, and the sawdust medium in which mushroom cultivation is completed can be utilized as compost or fuel.

The present invention is adapted to grow shiitake mushrooms after removing the moisture bag from the sawdust medium, so that the growth and growth of the shiitake mushroom spontaneously occurs smoothly.

1 is a block diagram showing a shiitake mushroom cultivation method according to the present invention;

1 is a block diagram showing a shiitake mushroom cultivation method according to the present invention, the present invention is a shiitake mushroom cultivation method using sawdust medium;

Stocking step in which the cultured sawdust medium is stocked into the grower after the mushroom spawn is inoculated;

Bag removal step in which the moisture bag of the sawdust medium stocked with the grower is removed and the sawdust medium is exposed to the outside;

A coating step in which the sawdust medium is coated with a moisturizing coating solution having moisturizing properties and antibacterial properties to form a moisturizing film;

It is intended to include; a growth step in which the sawdust medium with a moisturizing film is cultured to grow shiitake mushrooms.

At this time, the moisturizing coating solution consists of 100 parts by weight of the coating solution and 200 to 500 parts by weight of water, the coating solution consists of 100 parts by weight of the eco-friendly coating solution and 3 to 10 parts by weight of the ginkgo extract, and the eco-friendly coating solution is 5 to 20 wt. %, collagen 8~18wt%, lignin 5~20wt%, water absorbent 5~15wt%, surfactant 2~10wt%, stabilizer 3~15wt%, film forming agent 2~10wt%, water 20~50wt% has been

The stocking step is a step in which the primary cultured sawdust medium is supplied to the grower, and the sawdust medium is sterilized, inoculated and primary cultured after the sawdust medium is filled in a moisturizing bag (plastic bag) and sealed. it means.

That is, the present invention provides a sealing step of sealing after the sawdust medium is filled into a moisturizing bag (plastic bag) before the stocking step; Sterilization step of sterilization and cooling treatment after the sealing step; After the sterilization step, a part of the sealed moisturizing bag (plastic bag) is opened to form an opening, and an inoculation step of inoculating a mushroom seed into the sawdust medium through the opening; A lapping step in which the opening is sealed with a tape (vinyl tape) after the inoculation step; After the lapping step, it includes a primary culture step of culturing for 90 to 100 days.

The sealing step, the sterilization step, the inoculation step, the lapping step, and the primary culture step are well-known techniques in culturing shiitake mushrooms using sawdust medium, and thus detailed description thereof will be omitted.

In the bag removing step, the moisturizing bag (plastic bag) of the sawdust medium is removed so that the growth of the shiitake mushroom fruiting body is smoothly performed. In this way, when the moisturizing bag is removed, the sawdust medium is provided with a single lump of a certain shape. At this time, the sawdust medium is provided with various weight sizes such as 1.5 kg, 2.5 kg, 3.5 kg, etc., depending on the input amount put into the moisturizing bag. For example, the sawdust medium from which the moisturizing bag is removed may be formed into a solid mass having a diameter of about 12 cm and a height of about 40 cm.

The coating step is a step for forming a moisturizing film on the sawdust medium to prevent the moisture of the sawdust medium from drying out so that moisture management suitable for mushroom growth can be made. After this spray is sprayed, it is dried at room temperature for about 30 to 120 minutes to form a moisturizing film on the sawdust medium.

At this time, in the coating step, after immersing the sawdust medium in the moisturizing coating solution for 2 to 10 minutes, drying it at room temperature for about 30 to 120 minutes, as well as the sawdust located on the surface of the sawdust medium, as well as the sawdust located inside the moisturizing coating solution It is preferable to form a moisturizing film by

The moisturizing coating solution consists of a diluted coating solution by mixing 200 to 500 parts by weight of water with 100 parts by weight of the coating solution. Contains 3 to 10 parts by weight of Ginkgo biloba extract, which gives antibacterial function without affecting the moisture control function.

The eco-friendly coating solution is corrosive organic matter 5-20 wt%, collagen 8-18 wt%, lignin 5-20 wt%, water absorbent 5-15 wt%, surfactant 2-10 wt%, stabilizer 3-15 wt%, film 2-10 wt% of a film-former and 20-50 wt% of water.

The humic organic matter is a residue of plant decay in the ground for a long time, and fulvic acid or humic acid or a mixture of fulvic acid and humic acid is added.

The humic acid has a function of enhancing resistance to diseases and pests, and accumulating nutrients such as trace elements and organic matter to promote growth.

Such humic acid may use a commercially available product or may be directly manufactured and used. That is, the humic acid may be formed by fermenting and drying brewing waste, seaweed waste, molasses manufacturing waste, and other high-concentration organic waste liquid using a general composting method.

The fulvic acid (Fulvic acid) has the function of promoting the growth of shiitake mushrooms by promoting nutrient absorption, enhancing energy metabolism and enzyme activity.

The fulvic acid may be added in powder form, but is preferably extracted from humic acid, and liquid fulvic acid containing 20 to 50% by weight of fulvic acid may be added. That is, since the fulvic acid has a stronger acidity than humic acid, when the supersaturated concentrate extracted from humic acid (containing 20-50 wt% of fulvic acid) is added, it has the functions of humic acid and fulvic acid at the same time, and when compared with humic acid , it further promotes the growth of shiitake mushrooms. The extraction of the fulvic acid may be made by hot water extraction or ethanol extraction, preferably, hot water extraction is used.

In addition, the humic acid may be added in a mixture consisting of 20 to 100 parts by weight of fulvic acid, preferably 20 to 80 parts by weight of fulvic acid, based on 100 parts by weight of humic acid.

The humus organic material as described above not only promotes the growth of shiitake mushrooms, but also has a function of absorbing about 4 to 5 times its own weight and controlling moisture, thereby preventing moisture drying in sawdust medium. will do

When the amount of the humic organic matter is less than 5 wt%, the effect on the moisture-containing properties of the moisturizing film, the soil adsorption power and the film formation of the moisturizing film is insignificant, and when the content exceeds 20 wt%, the dispersibility of other components is weakened, The ability to form a moisturizing film is lowered.

The collagen (collagen protein) forms a strong film by mixing with a film forming agent, and the film formed in this way is biodegradable. That is, the collagen can achieve a high viscosity increase by mixing with other components, thereby forming a film according to fibrosis, and in particular, by mixing with a film forming agent, fibrosis can be increased, so that the Stability and physical properties are increased.

When the collagen is added in an amount of less than 8 wt%, it is difficult to form a strong film, and when it is added in excess of 18 wt%, the viscosity increases and liquid dispersion becomes difficult, so it must be added within an appropriate range.

The lignin is activated by an increase in temperature and has an adhesive function, so that it not only increases the firmness of the moisturizing film, but also is a natural polymer added to impart a degrading function to the moisturizing film, etc. are contained in large amounts.

That is, the present invention improves the cross-linking performance and soil adhesion of the moisturizing film by the addition of lignin (wood nitrogen), and has appropriate moisture permeability, thereby providing a very excellent plant growth effect.

When the amount of lignin is less than 5 wt%, the crosslinking performance improvement effect of the moisturizing film or the improvement of the soil adhesion effect is insignificant, and the biodegradability is reduced. When the content exceeds 20 wt%, the durability of the moisturizing film is reduced.

In addition, the lignin can be replaced by adding waste crops, and when the waste crops are added in this way, natural fiber is further added in addition to lignin, thereby further improving the durability of the moisturizing film.

The water absorbent has a water absorbent resin (water absorbant polymer) as a main component and is formed in the form of particles by mixing inorganic nutrients, growth promoters and electrolyte materials here, and is attached and fixed to the moisturizing membrane by lignin and a film forming agent. .

When the moisture absorbent absorbs moisture in a particle state, it changes to a gel state and absorbs and retains moisture up to 10 to 100 times its own weight, and when moisture is lost, it returns to the original particle state As one, propenamide propenoate copolymer, crosslinked polyacrylamide, polyacrylonitrile polymer, crosslinked sulfonated polystyrenes, alginate ) a selected one of the hydrogels is added.

When the water absorbent is added in an amount of less than 5 wt%, there is a problem that the properties of each component change when mixing other components and heating, and when added in excess of 15 wt%, the content of other components is affected In addition, it adversely affects the overall reaction.

The moisture absorbent having such a characteristic absorbs moisture without cracking the moisture absorbent film even if it absorbs moisture when the moisturizing film is formed.

The surfactant is intended to have a uniform liquid phase, and when it is added in an amount of less than 2 wt%, not only cannot expect an additive effect, but it is also difficult to use as a liquid, and when it is added in excess of 10 wt%, other components Not only does the content change, but the overall adhesion of the moisturizing coating agent is lowered, so it is added within an appropriate range. As such a surfactant, a known commercially available surfactant is used.

For example, the surfactant may be a nonionic, cationic, or anionic surfactant, preferably polyethylene glycol, polyhydric alcohol, polyethyleneamine, acetylene alcohol, a fluorinated alkyl group or a nonionic surfactant containing silicone. can be used.

The stabilizer (stabilizer) is to minimize the occurrence of deformation during heating, to maintain the quality of each component, phosphoric acid, monomethyl phosphoric acid, triphenyl phosphoric acid, trimethyl phosphoric acid, tributyl phosphoric acid, trioctyl phosphoric acid, monophenyl phosphoric acid , triphenylphosphoric acid and derivatives thereof, or phosphorous (P)-based stabilizers, phosphorous acid, triphenylphosphorous acid, trimethylphosphorous acid and derivatives thereof, and the like may be added.

When such a stabilizer is added in less than 3 wt%, there is a problem that the properties of each component change when mixing other components and heating, and when added in excess of 15 wt%, the content of other components is affected In addition, it adversely affects the overall reaction.

The film-former is added to form a moisturizing film with respect to the sawdust medium and to provide a function of delaying the evaporation of moisture through the formed moisturizing film. % is added.

A water-soluble polymer may be added to the film forming agent, and the water-soluble polymer is one of acrylic emulsion resin, vinyl acetate resin, polyurethane emulsion resin, polyvinyl alcohol, or a mixture of at least one thereof, preferably Polyvinyl alcohol is added.

The polyvinyl alcohol has adhesiveness, elasticity, flexibility and solubility, and not only facilitates the formation of a moisturizing film, but also has hot water resistance, so it has a function of preventing deformation and damage of the moisturizing film by hot water. , it also has a function of imparting biodegradability to the propellant function of collagen (danwon kyobaek) and the moisturizing film.

When such a film forming agent is added in an amount of less than 2 wt %, miscibility and moisture-retaining film functionality are lowered, and when added in an amount exceeding 10 wt %, a phenomenon in which permeability to the sawdust medium is reduced occurs.

In addition, the eco-friendly coating solution may further include 8 to 12 wt% of powdered activated carbon.

The powdered activated carbon is added to give a temperature control function to the sawdust medium, that is, a heat preservation function that the sawdust medium coated with a moisturizing film can be maintained at a constant growth temperature of shiitake, activated carbon, carbon black, graphite or its A mixture may be used, and it is more preferable to use active carbon, carbon black, or a mixture thereof in terms of effectiveness or economy.

When the powdered activated carbon is less than 8wt%, the expression of the warming effect is insignificant, and when the content exceeds 12wt%, it may inhibit the film formation and water dispersibility of the moisturizing film, which is not preferable.

The eco-friendly coating solution made as described above may be prepared by mixing and stirring corrosive organic matter, collagen, lignin, water absorbent, film forming agent and water, then adding a film forming agent and a stabilizer thereto and mixing and stirring.

The eco-friendly coating solution forms a primary mixture by lignin and water, and forms a secondary mixture by adding corrosive organic matter, moisture absorbent, and collagen to the primary mixture, and adding a film forming agent to the secondary mixture to form a tertiary After forming the mixed solution, a surface active agent and a stabilizer are added to the secondary mixture to form a quaternary mixture, and then, it is preferably prepared by cooling the quaternary mixture.

That is, the eco-friendly coating solution is prepared by adding water to lignin and stirring for about 40 to 50 minutes to form a first mixture, and after adding corrosive organic matter, moisture absorbent, and collagen to the first mixture, the temperature is heated to 85 to 100 ° C. After heating and stirring for 20 to 40 minutes to form a secondary mixture, a film forming agent is added to the secondary mixture and heating and stirring for 15 to 25 minutes to form a tertiary mixture, and then cooling the tertiary mixture to 70 to 85 ° C. , it can be prepared by adding a surfactant and a stabilizer, heating and stirring for 12 to 18 minutes to form a quaternary mixture, and then cooling the quaternary mixture to less than 40°C.

The ginkgo extract prevents bacterial propagation on sawdust medium, and gives antibacterial and control properties to protect from pests.

The ginkgo extract is a hot water extraction of ginkgo leaves or ginkgo biloba fruit or a mixture thereof (hereinafter referred to as 'ginkgo mixture'), and 100 parts by weight of ginkgo leaves or ginkgo biloba fruit or ginkgo mixture, 300 to 360 parts by weight of water. Concentrate by heating until about 1/4 to 1/3 of the liquid remains is used. At this time, the ginkgo leaves or the ginkgo biloba fruit or the ginkgo mixture except for the liquid are removed by a filtration network.

The water may be added at once and concentrated by heating, but preferably, 100 to 120 parts by weight of water is added to 100 parts by weight of ginkgo leaves or ginkgo biloba fruit or ginkgo mixture and heated to obtain about 1/4 to 1/3 of the liquid. After concentrating until Concentrate until about /4 to 1/3 to produce ginkgo extract.

For example, 1 ton of the ginkgo biloba fruit and 1 ton of water are added and concentrated by heating for about 45-50 hours, then 1 ton of water is added thereto and concentrated by heating for about 45-50 hours, and then 1 ton of water is added again. When it is added and concentrated by heating for about 45-50 hours, about 1 ton of ginkgo extract (liquid) is produced.

In addition, the ginkgo extract may be produced by hot water extraction of ginkgo leaves or ginkgo mixture, but it is preferable to produce by hot water extraction of ginkgo biloba fruit, especially ginkgo biloba, which are aged at room temperature for about 30 to 40 days after harvest. .

That is, when the phenol content in the sawdust medium increases, the growth of shiitake mushrooms may be inhibited, so it is preferable to control the phenol content in the sawdust medium. When comparing the phenol content in a ginkgo extract using ginkgo biloba and ginkgo biloba extract using ginkgo leaves or ginkgo mixture, the phenol content of the ginkgo extract using ginkgo biloba fruit is relatively low. When the extract is added, antibacterial and control properties are imparted without affecting the growth of shiitake mushrooms.

In particular, the phenol content of ginkgo extract using ginkgo biloba fruit one year after harvest is about 315.88∼324.65 μg/ml, whereas ginkgo biloba aged at room temperature (about 15℃∼25℃) for about 30 days immediately after harvest. The phenol content of the ginkgo extract using the tree fruit differs from about 18.06 to 25.05 μg/ml. more preferably.

Ginkgo biloba extract extracted with hot water as described above contains a number of flavonoids and organic acids known to have antibacterial action, and minerals such as Ca, Fe, Mg, Mn, Zn, etc. will promote the growth of

When the ginkgo extract made as described above is added in less than 3 parts by weight, the antibacterial and control functions are insignificant, and when added in excess of 10 parts by weight, it may adversely affect the growth of shiitake mushrooms. is added in

In addition, the coating undiluted solution made as described above is diluted with water to form a moisturizing film. That is, the moisturizing coating solution according to the present invention is diluted by adding 200 to 500 parts by weight of water to 100 parts by weight of the coating stock solution, and the components and content of the eco-friendly coating solution forming the coating stock solution and the content of ginkgo extract are the coating stock solution It is set taking into account the dilution of

The moisturizing coating solution made as described above is penetrated to the sawdust medium to form a moisturizing film, and the formed moisturizing film suppresses the evaporation of moisture in the sawdust medium from which the moisturizing bag is removed, so that moisture management until harvesting shiitake mushrooms is made. In addition to having a function, the shiitake mushroom has a physical property that can sufficiently penetrate the fruiting body, so that the growth of the shiitake mushroom can be performed smoothly.

In addition, the moisturizing film formed by the moisturizing coating liquid is made of a natural material or a biodegradable material, and after the formation of the moisturizing film, when about 3 months have elapsed for a certain period of time, natural decomposition is gradually made, but the moisture absorbent in the moisturizing coating liquid is originally installed It remains in the position, and by the remaining moisture absorbent, moisture, which is important for the growth of shiitake mushrooms, is continuously supplied, thereby preventing the deterioration of the moisture management function of the sawdust medium.

In addition, when the moisture film formed on the sawdust medium by the moisturizing coating solution absorbs moisture by the moisture absorbent, a gel-like moisture layer (moisture wall) is formed to protect the roots of shiitake mushrooms.

In addition, although there is a difference in the degree of dilution of the coating stock solution, the moisturizing film formed on the sawdust medium by the moisturizing coating solution starts to biodegrade after about 3 months, and after about 6 months, biodegradation of about 80% or more is made.

In addition, when a moisturizing film is formed on the sawdust medium by the moisturizing coating solution, the crushing phenomenon of the sawdust medium is prevented by the adhesiveness of the moisturizing film, and the growth of the shiitake is performed smoothly.

In the growth step, the shiitake mushroom is grown by inducing a fruiting body in a sawdust medium in which moisture is managed by a moisturizing film.

As a result of cultivating shiitake mushrooms by supplying moisture for about 5 to 10 minutes once every 3 to 5 days with respect to the sawdust medium on which the moisture film was formed as described above, shiitake mushrooms were grown every 2 to 3 weeks for about 3 to 5 months. The harvest was stable. Through this, when considering that moisture must be supplied once a day for about 5 to 15 minutes for moisture management of the sawdust medium from which the moisturizing bag has been removed, the moisture management ability of the sawdust medium with the moisturizing film according to the present invention is very high. can be seen to be excellent.

The present invention is not limited to the specific preferred embodiments described above, and without departing from the gist of the present invention as claimed in the claims, anyone with ordinary skill in the art to which the invention pertains can make various modifications. Of course, such modifications are intended to be within the scope of the claims.

Claims (13)

A method for cultivating shiitake mushrooms using sawdust medium;
Stocking step in which the cultured sawdust medium is stocked into the grower after the mushroom spawn is inoculated;
Bag removal step in which the moisture bag of the sawdust medium stocked as a grower is removed and the sawdust medium is exposed to the outside;
A coating step in which a moisturizing film is formed on the sawdust medium by a moisturizing coating solution having moisturizing properties and antibacterial properties;
A method of growing shiitake mushrooms, comprising: a growth step in which a sawdust medium with a moisturizing film is formed to grow shiitake mushrooms.
The method of claim 1 ;
In the coating step, the sawdust medium is immersed in the moisturizing coating solution for 2 to 10 minutes, and then dried at room temperature for 30 to 120 minutes to form a moisturizing film on the sawdust medium.
The method according to claim 1 or 2;
The moisturizing coating solution consists of 100 parts by weight of the coating undiluted solution and 200 to 500 parts by weight of water, the coating undiluted solution consists of 100 parts by weight of the eco-friendly coating solution and 3 to 10 parts by weight of the ginkgo extract, Collagen 8~18wt%, lignin 5~20wt%, water absorbent 5~15wt%, surfactant 2~10wt%, stabilizer 3~15wt%, film forming agent 2~10wt%, water 20~50wt% shiitake mushroom cultivation method.
4. The method of claim 3;
The humic organic matter is fulvic acid or humic acid, or a mixture of fulvic acid and humic acid.
5. The method of claim 4;
The fulvic acid is a liquid fulvic acid extracted from humic acid and containing 20 to 50% by weight of fulvic acid.
4. The method of claim 3;
Moisture absorbents include propenamide propenoate copolymer, crosslinked polyacrylamide, polyacrylonitrile polymer, crosslinked sulfonated polystyrenes, alginate ( alginate) shiitake mushroom cultivation method, characterized in that it is one selected from the hydrogel.
4. The method of claim 3;
The film-former is one of acrylic emulsion resin, vinyl acetate resin, polyurethane emulsion resin, polyvinyl alcohol, or a mixture of one or more thereof.
4. The method of claim 3;
The eco-friendly coating solution further contains 8 to 12 wt% of powdered activated carbon,
Powdered activated carbon is activated carbon (active carbon) or carbon black (Carbon Black), or a shiitake mushroom cultivation method, characterized in that a mixture of activated carbon and carbon black.
4. The method of claim 3;
For the eco-friendly coating solution, water is added to lignin and stirred for 40 to 50 minutes to form a first mixture, and corrosive organic matter, moisture absorbent, and collagen are added to the first mixture, and then 20 to 40 at 85-100 ° C. Minute heating and stirring to form a secondary mixture, adding a film forming agent to the secondary mixture and heating and stirring for 15 to 25 minutes to form a tertiary mixture, then cooling the tertiary mixture to 70-85° C., and surface active agent And a stabilizer is added to form a quaternary mixture by heating and stirring for 12 to 18 minutes, and then cooling the quaternary mixture to less than 40°C.
4. The method of claim 3;
Ginkgo extract is a shiitake mushroom cultivation method, characterized in that the hot water extraction of ginkgo leaves or ginkgo biloba fruit or a mixture of ginkgo leaves and ginkgo biloba fruit.
11. The method of claim 10;
Ginkgo extract is made by adding 300 to 360 parts by weight of water to 100 parts by weight of ginkgo leaves or ginkgo biloba fruit, or a mixture of ginkgo leaves and ginkgo biloba, and heating until the liquid becomes about 1/4 to 1/3. Characteristics of shiitake mushroom cultivation method.
4. The method of claim 3;
Ginkgo biloba extract is a shiitake mushroom cultivation method, characterized in that hot water extraction of ginkgo biloba fruits aged at room temperature for 30 to 40 days after harvest.
A shiitake mushroom, characterized in that it is cultivated and harvested by the shiitake mushroom cultivation method according to claim 1 or 2.

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