KR101509470B1 - Shiitake mushrooms cultivation method using biodegradable bags - Google Patents

Abstract

The present invention relates to a method of cultivating a mushroom using a biodegradable bag, and more particularly, to a method of cultivating mushroom by using a biodegradable bag which does not need to incise or remove the mushroom during the cultivation of mushroom, The present invention relates to a method of cultivating a mushroom using an environmentally friendly biodegradable bag, which facilitates the management of moisture of mushrooms and can utilize the waste paper after mushroom cultivation as compost or fuel without any additional treatment.
The method of cultivating mushroom using the biodegradable bag according to the present invention comprises: a step of preparing a medium by mixing sawdust and additives; A step of introducing the medium prepared in the medium preparation step into a synthetic resin bottle or a container; A sterilizing step of sterilizing the bottle into which the medium is introduced; A cooling step of cooling the sterilized bottle in a cooler; A step of inoculating the mushroom seedlings into the bottle into which the medium is introduced; A primary culture step in which the mushroom seedlings inoculated in the medium are cultured to form mycelium; A biodegradable bagging step of opening the bottle through the primary culture step to take out a primary culture of cultured mycelium, and then placing the primary culture in a biodegradable bag; A secondary culture step in which the primary culture is taken out from the bottle and then the cultured mycelium is collected and re-cultured in the process of being contained in the biodegradable bag; A browning step of subjecting the medium that has undergone the secondary culturing step to browning by being exposed to light; A fruiting body producing fruiting body in the browning-aged medium through the browning step; And a harvesting step of growing and harvesting the fruit bodies.

Description

[0001] The present invention relates to a method for cultivating shiitake mushrooms using biodegradable bags,

The present invention relates to a method of cultivating a mushroom using a biodegradable bag, and more particularly, to a method of cultivating mushroom by using a biodegradable bag which does not need to incise or remove the mushroom during the cultivation of mushroom, The present invention relates to a method of cultivating a mushroom using an environmentally friendly biodegradable bag, which facilitates the management of moisture of mushrooms and can utilize the waste paper after mushroom cultivation as compost or fuel without any additional treatment.

Shiitake mushrooms have been widely used as edible mushrooms in spring, summer and fall, occurring in the dry trees of hardwoods such as oak (oak, oak, and oak), chestnut, .

Shiitake mushrooms have been shown to reduce cholesterol in the blood stream to facilitate blood circulation and lower blood pressure to help prevent and treat atherosclerosis and heart disease. In recent years, it has been shown to contain anti-cancer and immunomodulatory compounds And it has been studied for health food and medicine, and various products using shiitake mushrooms are being developed.

Shiitake mushrooms are naturally grown in the natural environment and artificially cultivated. However, mushrooms are produced by artificial cultivation for a long time because of limited production. As a method of artificial cultivation of shiitake mushrooms, there has been used a lot of woody cultivation methods in which a hole is drilled through hardwood logs and inoculated with mushroom seeds. In recent years, in order to increase mushroom production efficiency and reduce labor, Sawdust cultivation methods in which the medium is packed in bottles or bags are popularized and used.

In order to carry out the sawdust cultivation of the shiitake mushroom as described above, Korean Utility Model Publication No. 20-2011-0005618, Korean Patent Publication No. 10-1132257, and Korean Patent Publication No. 10-0736882 disclose a method of cultivating sawdust cultivation in a synthetic resin bag However, in the case of cultivation using a conventional synthetic resin bag, as shown in FIG. 1, the bag must be opened and opened at the time when fruiting bodies are generated, and after the bag is opened, It is difficult to manage the mushroom with the moisture suitable for growing the mushroom, and after the mushroom cultivation is completed, the bag and the medium are not separated from each other and the medium must be discarded.

Korean Utility Model Publication No. 20-2011-0005618 Korean Patent Publication No. 10-1132257 Korean Patent Registration No. 10-0736882

In order to solve the above problems, the present invention uses a biodegradable bag which does not need to cut or remove a bag in the process of cultivating shiitake mushrooms, thereby facilitating moisture management of the medium until the mushroom is harvested, The present invention provides a method of cultivating mushroom using environmentally friendly biodegradable bags, which can be used as compost or fuel without any additional treatment.

In order to solve the above problems, a method of cultivating a mushroom using a biodegradable bag according to the present invention includes a step of preparing a medium by mixing sawdust and an additive; A step of introducing the medium prepared in the medium preparation step into a synthetic resin bottle or a container; A sterilizing step of sterilizing the bottle into which the medium is introduced; A cooling step of cooling the sterilized bottle in a cooler; A step of inoculating the mushroom seedlings into the bottle into which the medium is introduced; A primary culture step in which the mushroom seedlings inoculated in the medium are cultured to form mycelium; A biodegradable bagging step of opening the bottle through the primary culture step to take out a primary culture of cultured mycelium, and then placing the primary culture in a biodegradable bag; A secondary culture step in which the primary culture is taken out from the bottle and then the cultured mycelium is collected and re-cultured in the process of being contained in the biodegradable bag; A browning step of subjecting the medium that has undergone the secondary culturing step to browning by being exposed to light; A fruiting body producing fruiting body in the browning-aged medium through the browning step; And a harvesting step of growing and harvesting the fruit bodies.

In addition, the medium is composed of 78 weight percent of oak sawdust, 13 weight percent of rice bran, 3 weight percent of beet pulp, 3 weight percent of wheat bran, 2 weight percent of corncob and 1 weight percent of calcium carbonate, At room temperature to 98 to 102 ° C for 5 to 6 hours, or at 1.2 to atmospheric pressure for 120 to 122 ° C for 90 to 100 minutes, wherein the cooling step is performed at a temperature of 20 ° C And the culturing is continued until the second stage of cultivation, and the culturing is applied from the browning stage to the harvest stage.

The biodegradable bag may contain 70 to 80 wt% of PBAT (aliphatic-aromatic copolyester, polybutylene terephthalate terephthalate), 5 to 15 wt% of PLA (poly lactic acid polyurea), inorganic or other (CaCO 3) By weight and 5 to 15% by weight.

The method of cultivating the mushroom using the biodegradable bag according to the present invention uses the biodegradable bag for the cultivation of the mushroom so that the properties of the bag during the mushroom cultivation process are weakened and the fruit body can penetrate the bag by itself, Since the biodegradable bag inhibits moisture evaporation of the culture medium until harvesting the mushrooms without incision or removal, it is easy to manage the moisture of the culture medium, and the culture medium waste generated after the mushroom cultivation is treated without compaction or fuel It can be used as an environmentally friendly effect.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a process diagram showing a conventional method for cultivating a mushroom bag. FIG.
2 is a process diagram showing a cultivation method of shiitake mushroom using a biodegradable bag according to the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

2, a method of cultivating mushroom using a biodegradable bag according to an embodiment of the present invention starts from the step of preparing a medium to be inoculated with mushroom seeds by mixing sawdust and additives. The sawdust which can be used for producing the medium is various kinds of hardwood sawdust, but it is preferably oak sawdust. In one embodiment of the present invention, 78 weight percent of oak sawdust, 13 weight percent of rice bran, 3 weight percent of beet pulp, 3% by weight of wheat bran, 2% by weight of corn cob, and 1% by weight of calcium carbonate.

(210) In one embodiment of the present invention, the medium is introduced in an amount of 1100 cc. However, the amount of the medium to be fed is not limited to the capacity of the bottle or the container or the cultivation conditions . ≪ / RTI >

After the sterilization chamber is sterilized in the sterilization chamber at a temperature of 98 to 102 ° C for 5 to 6 hours or at a temperature of 120 to 122 ° C for 1.2 to atmospheric pressure for 90 to 100 minutes ), Cool down until the temperature of the culture medium is lowered to 20 ° C in a cooler to form the most suitable temperature for inoculating the mushroom seeds. (230)

When the cooling step is completed, the bottle is opened and the mushroom seedlings are inoculated into the medium (240). It is natural that the mushroom cultivation can be normally performed by inoculating the seedlings so that the medium is not contaminated.

Next, a primary culturing step is performed in which the inoculated bottle is closed and cultured so that a mycelium can be formed in the culture medium. (250)

The primary culture is carried out until the mushroom mycelium is fully mobilized in the culture chamber maintained at a temperature of about 20 to 25 DEG C, and the period of time to be performed may vary depending on the conditions. In an embodiment of the present invention, Week.

When the mycelium is allowed to grow on the medium by the primary culture, it becomes a period of rapid growth. In order to increase the growth of the mycelium, in order to increase the production amount of the mushroom, the bottle is opened and the primary cultured mycelium is taken out from the bottle. (260) The biodegradable bag comprises 70 to 80% by weight of PBAT (aliphatic-aromatic copolyester, polybutylene terephthalate terephthalate), 5 to 15% by weight of polylactic acid polylactic acid (PLA) (CaCO3), and the thickness of the biodegradable bag is preferably 20 占 퐉 or less so that the fruit body can penetrate the bag. In one embodiment of the present invention, the culture medium in which the primary culture has been incubated is packed in a biodegradable bag in a unit of 2 kg or 3 kg, but the amount of the pellet may vary depending on the conditions. The thinner the bag, the more difficult it becomes to handle. Alternatively, a method of removing the outer bag after incubation in a double manner may be used.

Next, the first culture is taken out of the bottle, and the mycelium that has accumulated in the biodegradable bag is scattered. Therefore, the second culture step is performed in which the mycelium is regrown and stabilized to stabilize the mycelium.

The second culture step is performed for about 2 weeks. During this period, the temperature of the culture medium is higher by 2 to 3 ° C than the room temperature while the culture medium is breathing. Therefore, the culture chamber should be thoroughly controlled at a temperature of about 20 ° C. Circulate and adjust the interval of the medium to prevent heat damage.

After completion of the secondary culture step, the medium is exposed to light to perform browning and maturation. (310) The step of aging the browning is a step for allowing the mycelium to form a protective film on its own, The quality of the medium is improved and the life of the medium is also increased.

When the browning process is completed, the culture is completed and fruiting bodies are generated in the medium to break through the biodegradable bag. (320) In the case of using the conventional synthetic resin bag in the filling step, When the bag is opened, it is difficult to keep water on the top surface of the medium so that the top surface of the bag is not dried. However, when the biodegradable bag is used as in the present invention, the cultivation process is performed It is not necessary to open the bag, since the biodegradable bag inhibits the evaporation of water in the culture medium until the cultivation is completed, so that the moisture management of the culture medium is easy .

(330) In one embodiment of the present invention, harvesting is carried out from the second culture stage to harvesting the mushroom from the second culture stage, The period up to the stage took 2 ~ 3 months, but it can be changed according to cultivation conditions.

The harvested medium can be harvested 4 to 5 times in the medium of 2 kg unit and 6 to 7 times in the medium of 3 kg depending on the weight of the medium after the recovery period, In the case of shiitake cultivation using a conventional synthetic resin bag, the bag and the culture medium are not separated after the harvest is completed unless the bag and the culture medium are separated during the cultivation process. However, since the biodegradable bag is used in the present invention, the waste paper waste can be utilized as compost or used as a fuel after drying without removing the bag during cultivation.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, The present invention is not limited to the embodiments and the accompanying drawings.

110: Medium preparation step 210: Entry step
220: sterilization step 230: cooling step
240: Inoculation step 250: Primary culture step
260: biodegradable encapsulation step 270: secondary culture step
310: browning and ripening step 320: fruit body generating step
330: Harvesting stage

Claims (3)

A medium preparation step (110) for preparing a medium by mixing sawdust and additives; A step 210 of injecting the medium prepared in the medium preparation step 110 into a synthetic resin bottle or a container; A sterilization step (220) for sterilizing the bottle into which the culture medium is introduced; A cooling step (230) for cooling the sterilized bottle in the chiller; An inoculation step (240) of inoculating the mushroom seedlings into the bottle into which the medium is introduced; A primary culture step (250) in which the mushroom seedlings inoculated in the culture medium are cultured to form a mycelium; A biodegradable encapsulation step (260) of opening the bottle through the primary culture step to take out the primary culture cultured in the mycelium and then placing the primary culture in a biodegradable bag; A secondary culture step 270 in which the primary culture is taken out from the bottle and packed in a biodegradable bag and the cultured mycelium is re-cultured; A browning step (310) of exposing the medium through the secondary culture step (270) to browning; A fruit body generating step 320 in which a fruit body is generated in a browning-aged medium through the browning step 310; And a harvesting step (330) of growing and harvesting the fruit bodies,
The biodegradable bag comprises 70 to 80% by weight of PBAT (aliphatic-aromatic copolyester, polybutylene terephthalate terephthalate), 5 to 15% by weight of PLA (poly lactic acid polyurea), an inorganic material or calcium carbonate (CaCO₃) 5 To 15% by weight, and the thickness thereof is 20 [micro] m or less so that the fruiting body can penetrate through the bag. The method of cultivating the mushroom using the biodegradable bag. The method according to claim 1,
The medium consisted of 78 weight percent oak sawdust, 13 weight percent rice bran, 3 weight percent beet pulp, 3 weight percent wheat bran, 2 weight percent corncob, and 1 weight percent calcium carbonate,
The sterilization step 220 is performed at a temperature of 98 to 102 ° C at a normal pressure in the sterilization chamber for 5 to 6 hours or at 120 to 122 ° C for 90 to 100 minutes at a pressure of 1.2 atm,
The cooling step 230 is performed in the cooler until the temperature of the medium is lowered to 20 캜,
Wherein the cultivation is carried out from the step of entering 210 to the second culturing step 270 and the cultivation is applied from the browning step 310 to the harvesting step 330. In the biodegradable bag, . delete

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