KR101239711B1 - Manufacturing process of lentinus edodes culture - Google Patents

Abstract

PURPOSE: A manufacturing method of Lentinus edodes culture material is provided to bottle a solid medium in a wide-mouth bottle, inject liquid fungus, culture the fungus, take the solid medium out of the bottle, put the medium into a bag, and culture the medium. CONSTITUTION: A manufacturing method of Lentinus edodes culture material comprises the following steps: (A) Manufacturing a solid fungus by mixing 100 wt% of sawdust and 10-40 wt% of rice bran and adding 150-250 wt% of water; (B) putting the solid fungus in a wide-mouth bottle, sterilizing and cooling the same at 100-135 deg.C. for 2-3 hours in a sterilizing room; (C) injecting 5-15 wt% of Lentinus edodes culture medium into the solid fungus based on 100 wt% of sawdust, and culturing the medium at 15-25 deg.C. for 40-55 days under a dark condition; and (D) taking the cultured material out of the wide-mouth bottle, putting the cultured material into a porous bag, and bottle-culturing the cultured material at 15-25 deg.C. for 45-60 days. The Lentinus edodes culture medium comprises: 100 wt% of water, 2-5 wt% of sugar, 0.2-0.5 wt% of yeast extract, 0.03-0.1 wt% of KH2PO4, 0.03-0.1 wt% of MgSO4.7H2O, and 0.05-0.2 wt% of KNO3. 10-20 wt% of wheat bran per 100 wt% of sawdust is additionally added to the solid fungus of step (A). 5-10 wt% of cotton seed hull per 100 wt% of sawdust is additionally added to the solid fungus of step (A). The humidity for putting the culture material in the porous bag in step (D) is 1-60%.

Description

Method of manufacturing shiitake mushroom culture {Manufacturing Process of Lentinus edodes Culture}

The present invention mushroom shiitake ( Lentinus edodes ) relates to a method for producing a culture. Specifically, the present invention relates to a method for producing shiitake mushroom culture, characterized in that the solid medium is bottled into a photocatalyst, inoculated and cultured with a liquid spawn, and then the bottle is incubated and cultured again in a bag.

Shiitake mushroom is a fungus of the genus Pleurotus eryngii Ottoaceae, which occurs in dry trees of broad-leaved trees such as oaks, chestnuts, and roe trees in spring and autumn. Mushroom shade 4-10 cm in diameter, initially hemispherical, but gradually flattened. The surface of the shade is dark brown and covered with dark brown thin fluffy scaly pieces, sometimes bursting to show white flesh. Artificial cultivation is performed by wood and is distributed in Korea, Japan, China and Taiwan.

Shiitake mushrooms have been studied for a long time in China. Shiitake mushrooms have a substance called eridadamine, which lowers cholesterol levels in blood. In addition, the blood pressure lowering action is also suitable for the prevention of high blood pressure and arteriosclerosis. Eridademin dissolves dried mushrooms in water, so use the juice without throwing it away. It is also rich in vitamins B1 and B2. Shiitake mushroom's rich flavor is guanylic acid and is a component of nucleic acid seasoning. The fragrance is based on wrenches. In addition, shiitake mushrooms contain a lot of ergosterol, which has the effect of vitamin D, and when the body receives ultraviolet rays, it turns into vitamin D. On the other hand, it was also found that vitamin B12, which was not known to exist in plants, is present in the altitude. About 600 years ago, during the Ming Dynasty, a man named Wu Xi called the effect of shiitake feng shui. To solve this modernly, the spores of shiitake mushrooms have an ingredient that controls the flu and cancer winds these days. And when you put shiitake mushrooms in various vegetable dishes, it tastes better than meat. This is because it contains guanylic acid, which is the strongest rich ingredient.

On the other hand, the use of liquid spawn in the cultivation of mushrooms is known to have high growth efficiency because the medium can be utilized evenly in the solid medium compared to the solid spawn. However, inoculating liquid seed when cultivating shiitake mushrooms has a problem of high contamination rate by other bacteria before the mycelia are completely spread throughout the solid medium, and the amount of shiitake mushrooms produced is much less. For this reason, in the case of shiitake mushrooms, less than half the production and even longer adaptation period, solid spawn is still used.

KR 2011-0037447 Hana Biotech Co., Ltd., agricultural company 2011.04.13. KR 1988-0005854 Kim Won Hong 1988.07.21).

The present invention has been made in order to solve the problems described above, after entering the solid medium into the bulb, inoculated and incubated with the liquid spawn, the disease is characterized by culturing by desalting and encapsulating the solid medium again. It is an object to provide a method for producing a culture.

Shiitake mushroom culture method of the present invention to achieve the object as described above,

(A) mixing 100 parts by weight of sawdust and 10 to 40 parts by weight of rice bran, and adding 150 to 250 parts by weight of water to prepare a solid medium,

(B) sterilizing the solid medium in a photosphere bottle for 2 to 3 hours at 100 to 135 ℃ and cooling in a sterilization chamber,

(C) inoculating 5 to 15 parts by weight of the mushroom culture based on 100 parts by weight of the sawdust in a sterilization chamber to the solid medium and cancer culture at 15 to 25 ℃ for 40 to 55 days, and

(D) taking the cultured cultured cultures from the photocatalysts and putting them back into a porous bag for bright culture at 15 to 25 ° C. for 45 to 60 days

Characterized in that it comprises a.

In addition, the shiitake mushroom culture method of the present invention may further comprise 10 to 20 parts by weight of bran per 100 parts by weight of sawdust in the solid medium of the step (A).

In addition, the shiitake mushroom culture method of the present invention may further comprise 5 to 10 parts by weight of cotton seed hull per 100 parts by weight of sawdust in the solid medium of the step (A).

In addition, the mushroom culture of step (C)

100 parts by weight of water,

2 to 5 parts by weight of sugar,

0.2 to 0.5 parts by weight of soybean meal or yeast extract,

0.03 to 0.1 parts by weight of KH ₂ PO ₄ ,

0.03 to 0.1 part by weight of MgSO ₄ 7H ₂ O, and

KNO ₃ 0.05 to 0.2 part by weight

It is preferable to culture the shiitake mushroom spawn in a liquid medium containing.

In addition, the mushroom culture solution of the step (C) is preferably subjected to a seed test before inoculating the solid medium.

In addition, the shiitake mushroom culture method of the present invention preferably has a humidity of 1 to 60% when the cultured cultured in the step (D) back to the porous bag.

In addition, the shiitake mushroom culture method of the present invention preferably has a temperature of 10 to 20 ℃ when the culture cultured back in the porous bag in step (D).

In addition, the light culture of the step (D) is preferably roughness of 150 to 400 Lux.

In addition, the humidity in the bright culture of the step (D) is preferably 50 to 70%.

In addition, the sawdust is preferably oak sawdust.

Shiitake mushroom culture method of the present invention has the advantage that the sterilization can be carried out perfectly by including the step of placing the mouthpiece in the mouth before the step of placing the conventional solid medium directly into the bag. This has the effect of shortening the working time required for the admission and sealing, as well as the effect of significantly lowering the contamination rate by various bacteria. This pollution reduction effect is limited to perfect sterilization, but because the shiitake mushroom mycelium has already grown enough in the mad cow disease, shiitake mushrooms can form a dominant species at the beginning of solid medium culture, which can sufficiently suppress the growth of various bacteria.

In addition, the present invention is provided with a mycelium already grown enough in the solid medium as described above has the effect of shortening the total culture days from the standpoint of farmers to purchase the solid medium of the present invention to cultivate shiitake mushrooms. Specifically, when inoculating the conventional solid spawn takes 3 to 4 months to completely spread the mycelium throughout the solid medium, shiitake mushrooms could not be harvested during this period, but inoculated with liquid spawn as in the present invention. It takes only one to two weeks for the mycelia to spread throughout the solid medium, which greatly improves productivity.

The present invention is also capable of commercial production of at least 11 to 17 times per solid medium, the efficiency is significantly increased compared to the commercial production of up to six times the conventional solid seed inoculation method. In addition to the perfect sterilization, securing the predominant species status of shiitake mushrooms, and the efficiency of liquid spawn, it is possible to supply enough oxygen to the inside of the solid medium by de-bleaching and mixing the firstly introduced solid medium. It seems to be because of losing.

1 is a photograph taken the step of mixing the solid medium material.
Figure 2 is a photograph of the admission step of placing a solid medium in the photosphere bottle.
Figure 3 is a photograph of the step of sterilizing and cooling the bulb bottle.
Figure 4 is a photograph of the step of inoculating liquid seed spawns.
Figure 5 is a photograph of the step of culturing in a mad mouth disease.
Figure 6 is a photograph taken of the demineralization step to take out the solid medium from the bulb mouth.
Figure 7 is a photograph of the encapsulation step of placing a solid medium in the bag.
8 is a photograph of the step of culturing in a bag.
Figure 9 is a photograph of the culture medium is completed and waiting for pre-sale.

Hereinafter, preferred embodiments of the present invention will be described in detail. In the following description, numerous specific details, such as specific elements, are set forth in order to provide a thorough understanding of the present invention, and it is to be understood that the present invention may be practiced without these specific details, It will be obvious to those who have knowledge of. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

The method of producing shiitake mushroom culture of the present invention first starts by mixing 100 parts by weight of sawdust and 10 to 40 parts by weight of rice bran, and adding 150 to 250 parts by weight of water to prepare a solid medium. Here, the sawdust is particularly preferably oak mushroom cultivation oak sawdust. The solid medium may further include 10 to 20 parts by weight of bran per 100 parts by weight of sawdust, and may further include 5 to 10 parts by weight of cotton seed hull.

The solid medium prepared in this way must first undergo a sterilization step before inoculating shiitake mushroom seed. In the present invention, the solid medium is placed in a photosphere bottle and sterilized at 100 to 135 ° C. for 2 to 3 hours to maximize the sterilization effect. This sterilized solid medium was cooled to room temperature in the sterilization chamber.

Separately, the solid medium of the present invention is characterized by inoculating liquid seed instead of inoculating solid seed. In general, the liquid spawn is known to have high growth efficiency because the spawn mushroom can be used in a wider area than the solid spawn because the shiitake mushroom can utilize a medium in a wide area. However, the inoculation of liquid spawn during shiitake mushroom cultivation showed that the rate of contamination by other bacteria was high, and the amount of shiitake mushrooms produced was much lower before the mycelia were completely spread throughout the solid medium. This is due to the nutrients in the liquid medium, other bacteria grow in the solid medium first, the shiitake mushroom loses the status of dominant species, and as a result, the efficiency is reduced as it grows in competition with other bacteria.

However, the present invention significantly lowers the possibility of contamination of various bacteria by solid sterilization process by first placing in a bottle, before the solid medium is put into a bag, as a result it is possible to grow only shiitake mushrooms alone.

Specifically, the method of producing a liquid seed in the present invention first starts from the step of obtaining the progeny. After preparing a plate medium containing PDA (Potato dextrose agar) in a petri dish, cut shiitake mushrooms having a diameter of 2 to 7 cm to have an average corner length of 1 to 7 mm, and the sections were aseptically plated. Transplant to. After culturing the Petri dish transplanted with tissue for 10 to 15 days at 20 to 25 ℃, cut the grown shiitake mycelium with a plate medium so that the average length is 5 to 10 mm, transferred to a new PDA plate medium and cultured , Repeat this process 1 to 2 more times to obtain shiitake mushroom bacteria.

2 to 5 parts by weight of sugar, 0.2 to 0.5 parts by weight of soybean meal or yeast extract, 0.03 to 0.1 parts by weight of KH ₂ PO _4, 0.03 to 0.1 parts by weight of MgSO ₄ .7H ₂ O, and KNO ₃ 0.05 to 100 parts by weight of water. To 0.2 part by weight to prepare a liquid medium and sterilize it, and then cooled in aseptic state and put in a flask or Ringer's bottle, 2 to 5 pieces of prokaryotic fragment with an average length of 5 to 10 mm obtained with the plate medium Inoculated and incubated for 20 to 25 days in a shaker at 20 to 25 ℃. Scale it up again and incubate the liquid spawn in the tank. In this tank culture, it is preferable to pressurize 5 to 30% to sufficiently inflate air.

It is preferable to inspect the culture solution under a microscope at about 18 to 23 days of tank culture to see if bacteria are present and whether the clamp state of the seed is appropriate.

The liquid spawn prepared in this way, that is, the mushroom culture solution is inoculated into the solid medium in the bulb mouth bottle by 5 to 15 parts by weight based on 100 parts by weight of the sawdust in a sterilization chamber and cultured at 15 to 25 ° C. for 40 to 55 days. As the culture proceeds during such cancer culture, it is preferable to manage the carbon dioxide concentration in the culture chamber so that it does not exceed 1000 to 3000 ppm.

These cultured cultures, that is, the solid medium and shiitake mycelium mixture is removed from the photosphere bottle, mixed and put back into the porous bag and incubated for 45 to 60 days at 15 to 25 ℃. By mixing the solid medium once, sufficient oxygen is supplied to the medium, and through this, cells can be actively grown without restriction due to oxygen in bright culture.

In this case, when the cultured cultured cancer is returned to the porous bag, the humidity is preferably 1 to 60%. If the humidity exceeds 60%, the possibility of contamination by various bacteria is increased. And, the temperature is also 10 to 20 ℃ is preferable for growing shiitake mushrooms without lag time (lag time). In addition, the light intensity of the light culture is preferably 150 to 400 Lux, the humidity of the light culture is preferably 50 to 70%. In addition, it is preferable to manage not to exceed 1000 to 3000 ppm when the carbon dioxide concentration in the culture chamber increases as the culture proceeds as in the case of cancer culture.

Hereinafter, embodiments of the present invention will be described.

Example

Example  -Tissue separation

After preparing a plate medium containing PDA (Potato dextrose agar) in Petri dishes, shiitake mushrooms having a diameter of 5 cm were cut to have an average edge length of 5 mm, and the sections were transplanted to the plate medium under aseptic conditions. After incubating the petri dish with tissues for 12 days at 25 ° C, cut the grown shiitake mushroom mycelium along with the plate medium to an average length of 7 mm, transfer it to a new PDA plate medium, and incubate this process once. It was repeated further to obtain shiitake progeny.

Example  -Liquid culture (1)

500 ml of distilled water, 17 g of sugar, 2 g of soybean meal, 0.4 g of KH ₂ PO _4, 0.4 g of MgSO ₄ · 7H ₂ O, 0.6 g of KNO ₃ were added and mixed in a 1 l container to prepare a liquid medium, which was then autoclaved. After sterilization at (120 ° C.) for 1 hour, 4 cells of 7 mm in average length obtained with the plate medium were inoculated after cooling under aseptic conditions, and incubated in a shaker at 25 ° C. for 25 days.

Example  -Liquid culture (2)

500 l of distilled water, 17 kg of sugar, 2 kg of soybean meal, 400 g of KH ₂ PO _4, 400 g of MgSO ₄ · 7H ₂ O, 600 g of KNO ₃ were added and mixed in a 1000 l tank to prepare a liquid medium, which was 120 ° C. After sterilization for 2 hours at, inoculated with 500 ml of the shiitake mushroom culture obtained after cooling in a sterile state, and incubated for 25 days at 25 ℃.

Example  - Cancer culture

10 kg of oak sawdust, 1.5 kg of rice bran, 1.5 kg of bran, and 20 kg of water were added to the blend, and then 700 g each of the bulbs, sterilized at 120 ℃ for 100 minutes and cooled, Medium was prepared. In a sterile chamber, 20 cc of shiitake mushroom culture was inoculated into one solid medium of the photosphere bottle, and cancer-cultured at 20 ° C. for 45 days.

Example  - Culture

The shiitake mushroom culture, which was mixed with shiitake mycelium and a solid medium, was taken out of the bulb, and then mixed. Then, the shiitake mushroom culture was placed in a bag formed with 70 through holes, and brightly incubated at 20 ° C. for 50 days.

The shiitake mushroom culture of the present invention incubated in this way takes only 1 to 2 weeks to completely spread the mycelium throughout the solid medium, it was confirmed that commercial culture is possible at least 11 to 17 times in one solid medium.

Comparative example  One: Solid spawn  culture

After preparing a plate medium containing PDA in Petri dishes, shiitake mushrooms having a diameter of 5 cm were cut to have an average edge length of 5 mm, and the sections were transplanted to the plate medium under aseptic conditions. After culturing the Petri dishes with tissues for 12 days at 25 ° C, cut the grown shiitake mycelium with plate media to an average length of 7 mm, transfer them to new PDA plate media, and incubate this process once. It was repeated further and the shiitake progeny were obtained.

Then, 10 kg of oak sawdust, 1.5 kg of rice bran, and 1.5 kg of bran were added, and 20 kg of water was added to the blend, followed by sterilization at 120 ° C. for 100 minutes, followed by cooling to prepare a solid medium. The solid medium was placed in a bag formed with 70 through holes in a sterilization chamber and inoculated with 4 pieces of shiitake fungus fragments having an average length of 7 mm obtained with the plate medium, and brightly cultured at 20 ° C. for 70 days.

Shiitake culture cultured in this way it takes 3 to 4 months to completely spread the mycelia throughout the solid medium, it was confirmed that commercial culture is possible only up to six times in one solid medium.

Comparative example  2: Liquid spawn  culture

After preparing a plate medium containing PDA (Potato dextrose agar) in Petri dishes, shiitake mushrooms having a diameter of 5 cm were cut to have an average edge length of 5 mm, and the sections were transplanted to the plate medium under aseptic conditions. After culturing the Petri dishes with tissues for 12 days at 25 ° C, cut the grown shiitake mycelium with plate media to an average length of 7 mm, transfer them to new PDA plate media, and incubate this process once. It was repeated further and the shiitake progeny were obtained.

500 ml of distilled water, 17 g of sugar, 2 g of soybean meal, 0.4 g of KH ₂ PO _4, 0.4 g of MgSO ₄ · 7H ₂ O, 0.6 g of KNO ₃ were added and mixed in a 1 l container to prepare a liquid medium, which was then autoclaved. After sterilization at (120 ° C.) for 1 hour, 4 cells of 7 mm in average length obtained with the plate medium were inoculated after cooling under aseptic conditions, and incubated in a shaker at 25 ° C. for 25 days.

10 kg of oak sawdust, 1.5 kg of rice bran, and 1.5 kg of bran were added, and 20 kg of water was added to the blend, followed by sterilization at 120 ° C. for 100 minutes, followed by cooling to prepare a solid medium. The solid medium was placed in a bag formed with 70 through holes in a sterilization chamber, inoculated with 100 cc of shiitake mushroom culture, and incubated at 20 ° C. for 70 days.

The culture of shiitake mushrooms is more than half contaminated by other bacteria before the mycelia are completely spread throughout the solid medium, and the amount of shiitake mushrooms produced in one solid medium is less than 10% of the embodiment of the present invention. It was confirmed that no.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Of course it is possible. Accordingly, the scope of the present invention should not be construed as being limited to the above-described embodiments, but should be determined by equivalents to the appended claims, as well as the following claims.

Claims (5)

(A) mixing 100 parts by weight of sawdust and 10 to 40 parts by weight of rice bran, and adding 150 to 250 parts by weight of water to prepare a solid medium,
(B) sterilizing the solid medium in a photosphere bottle for 2 to 3 hours at 100 to 135 ℃ and cooling in a sterilization chamber,
(C) inoculating 5 to 15 parts by weight of the mushroom culture based on 100 parts by weight of the sawdust in a sterilization chamber to the solid medium and cancer culture at 15 to 25 ℃ for 40 to 55 days, and
(D) taking the cultured cultured cultures from the photocatalysts and putting them back into a porous bag for bright culture at 15 to 25 ° C. for 45 to 60 days
Including,
Mushroom culture of step (C) is
100 parts by weight of water,
2 to 5 parts by weight of sugar,
0.2 to 0.5 parts by weight of soybean meal or yeast extract,
0.03 to 0.1 parts by weight of KH ₂ PO ₄ ,
0.03 to 0.1 part by weight of MgSO ₄ 7H ₂ O, and
KNO ₃ 0.05 to 0.2 part by weight
Shiitake mushroom culture method comprising culturing shiitake spawn seed in a liquid medium containing a. The method according to claim 1,
Shiitake mushroom culture method, characterized in that it further comprises 10 to 20 parts by weight of bran per 100 parts by weight of sawdust in the solid medium of step (A). The method according to claim 1,
Shiitake mushroom culture method, characterized in that it further comprises 5 to 10 parts by weight of cotton seed hull per 100 parts by weight of sawdust in the solid medium of step (A). delete The method according to claim 1,
Shiitake culture method of producing a culture, characterized in that the humidity of the culture cultured in the step (D) back to the porous bag 1 to 60%.

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