KR20200061556A - A Method for Cultivating a White Shiitake Mushroom Containing a Selenium - Google Patents

Abstract

The present invention relates to a cultivation method of white shiitake mushroom containing selenium and, specifically, to a cultivation method of white shiitake mushroom containing selenium, capable of maintaining the unique taste and effectiveness of selenium by absorbing selenium in the cultivation process. The cultivation method of white shiitake mushroom containing selenium comprises: a step of forming a medium by mixing raw materials for inoculation of mushroom spawn; a step of sterilizing and inoculating the medium; a step of forming at least one hole in the medium depending on the growth of the mycelia while culturing the spawn; a step of setting different temperature conditions depending on vertical layers while humidifying the medium; and a step of growing mushroom by removing the packaging of the medium while controlling the humidity to harvest the mushroom.

Description

A Method for Cultivating a White Shiitake Mushroom Containing a Selenium}

The present invention relates to a method for cultivating selenium-containing baekhwago, and specifically, to a method for cultivating selenium-containing baekhwago so that selenium is absorbed during the cultivation process to maintain the unique taste and efficacy of selenium.

Shiitake mushrooms, which are edible mushrooms that can be artificially grown, can be divided into a white flower growing in a low humidity state and a black flower growing in a high humidity state. Artificial cultivation of shiitake mushrooms is known to inoculate the seed germs of mushrooms by drilling holes in logs. Shiitake mushrooms are known to be effective ingredients for making mushroom soup with excellent taste and aroma and low moisture content. A technique in which various components are added in the cultivation process of shiitake mushrooms is known in the field. Patent Publication No. 10-2017-0079322 discloses a method for cultivating seedling mushrooms containing selenium containing selenium excellent in anticancer function in fruiting bodies. In addition, Patent Publication No. 10-2005-0080111 discloses a method for cultivating selenium baekhwago shiitake mushrooms grown by inoculating seed germ in a medium of nutrients containing selenium and oak sawdust immersed in an aqueous solution thereof. In the process of cultivation of mushrooms, growth conditions can be a major factor in determining the quality of mushrooms to be harvested, and supply conditions of nutrients such as selenium can be a major factor influencing intake. However, the prior art does not disclose such growth conditions or supply conditions.

The present invention is to solve the problems of the prior art has the following purposes.

Prior Art 1: Patent Publication No. 10-2017-0079322 (Gangwon World Farm Co., Ltd., published on July 10, 2017) Method for growing sesame mushroom containing selenium Prior Art 2: Patent Publication No. 10-2005-0080111 (Yoon Moo-Won, published on August 11, 2005) Selenium white walnut shiitake mushroom cultivation method

It is an object of the present invention to provide a method for cultivating selenium-containing baekhwago, in which the hyphae is sturdy as the defect rate is reduced by maintaining different temperature conditions according to vertical layers in the growth process.

According to a preferred embodiment of the present invention, the method for cultivating selenium-containing baekhwago comprises the steps of mixing the raw materials for inoculation of mushroom spawn to form a medium; Sterilizing the medium and inoculating; Forming at least one hole in the medium according to the growth of the mycelia while culturing the seed bacteria; Setting different temperature conditions according to vertical layers while humidifying the medium; And removing the packaging of the medium to grow while controlling the humidity and harvesting.

According to another suitable embodiment of the present invention, the veggies include sawdust shaped bran flakes, bran, cottonseed shell, gypsum and selenium complex.

According to another suitable embodiment of the invention, the humidification is such that the water surface is formed above the medium while the temperature of the upper layer forming different layers is maintained at a higher temperature than the lower layer.

The cultivation method of selenium-containing baekhwago according to the present invention is such that nutrients are absorbed into the human body during the ingestion process by injecting germanium or selenium complex with water into the medium to effectively absorb these nutrients in the growth process. The cultivation method according to the present invention makes it possible to harvest robust mushrooms while reducing the defective rate of mushroom cultivation. In addition, the cultivation method according to the present invention has the advantage of making the mushroom flesh softer while having a strong aroma and a large mushroom cluster.

1 shows an embodiment of a method for cultivating selenium-containing baekhwago according to the present invention.

The present invention will be described in detail below with reference to the embodiments presented in the accompanying drawings, but the embodiments are intended for a clear understanding of the present invention and the present invention is not limited thereto. In the following description, components having the same reference numerals in different drawings have similar functions, and are not described repeatedly unless necessary for understanding of the invention, and well-known components are briefly described or omitted, but the present invention It should not be understood as being excluded from the embodiment.

1 shows an embodiment of a method for cultivating selenium-containing baekhwago according to the present invention.

Referring to Figure 1, the cultivation method of selenium-containing baekhwago comprises the steps of forming a medium by mixing the raw material for inoculation of mushroom spawn (P11); Sterilizing the medium (P12) and inoculating (P13); Forming at least one hole in the medium according to the growth of the mycelia while culturing the seed bacteria (P14); Setting different temperature conditions according to vertical layers while humidifying the medium (P15); And removing the packaging of the medium and growing while controlling the humidity to harvest (P16).

The cultivation of baekhwago can be performed in various spaces in which growth conditions can be controlled, for example, in a plant farm for mushroom cultivation, a mushroom house, a container adapted for cultivation of mushrooms, or a variety of similar places. For cultivation of mushrooms, a medium must be made, and the medium may be a basic unit in which mushroom spawns are inoculated and grown. The medium may be made of a receiving container made of various materials and a raw material accommodated inside the receiving container. The receiving container may have, for example, a diameter of 10 to 40 cm; A thickness of 20 to 80 cm; And it may be a bag shape made of a vinyl material or a similar material having a thickness of 0.002 to 0.15 mm, but is not limited thereto. When the receiving container is prepared, the raw material may be blended and filled into the receiving container to form a medium (P11). Raw materials may include, but are not limited to, sawdust-shaped shavings, bran, cottonseed husks, gypsum and selenium complexes. For example, the raw material is 10 to 30 wt% of bran; 0.1 to 15 wt% cottonseed peel; 1 to 5 wt% gypsum; Selenium complex of 10 to 5000 ppm; And sawdust-shaped shavings that make the whole 100 wt%. Wheat bran or cottonseed husks can be pulverized into a pulverizer to form a powder, and gypsum can be added in a powder form. Gypsum can be, for example, various types of gypsum, such as fiber gypsum, calcined gypsum, dialysis gypsum, potassium sulfate or phosphate gypsum, while simultaneously neutralizing raw materials and simultaneously being absorbed by mushrooms to prevent headaches or fever, for example. It has the function of generating various health promoting effects. In addition, gypsum absorbs heavy metal components so that the raw materials are kept in a state suitable for cultivation of mushrooms. The selenium complex may include germanium or a similar mineral component, and the selenium complex may have an antioxidant function and have an effect of preventing various types of cancer. The selenium complex can be added in a variety of ways, for example by adding fermented brown rice products as a raw material. For example, rice rice and rice bran can be separated from brown rice and fermented with lactic acid bacteria to be added as a raw material, and instead of wheat bran, cottonseed husks, or debris, or put together with these materials into a storage container. For example, rice bran or rice grains of brown rice or similar grains are separated to become liquid fermentation or solid fermentation, and the active ingredient may be separated from the centrifuge. Subsequently, 3 to 10 wt% of the total weight of the fermentation component may be added in lieu of debris, bran or cottonseed husk in an amount corresponding thereto. Alternatively, the selenium complex can be made by dissolving inorganic selenium containing selenium in ether and adding it to water to mix it. The selenium complex may include 1 to 10 wt% of germanium, 1 to 5 wt% of potassium, or 1 to 5 wt% of phosphoric acid component of the total weight of the complex. The selenium composite can be made in a variety of ways and is not limited to the examples presented. When the raw material is prepared in this way, the medium can be made into a sealed container by mixing it uniformly with water in an amount of 40 to 80 wt% based on the weight of the raw material, and sealing it. Multiple media can be made in this way, and multiple media can be made through a mass production process. Each medium can be 1 to 10 kg, and mushrooms can be grown in each medium. For the growth of mushrooms, a plurality of mediums may be accommodated inside a sterilization device or sterilized while being transported through a conveying means such as a conveyor device (P12). Sterilization may be performed by a high temperature sterilization method, a steam sterilization method, or a similar method, but is not limited thereto. In this way, the sterilized medium may be transferred to the mushroom medium, and various control facilities for adjusting the growth conditions of the mushrooms may be disposed on the mushroom medium.

A number of media can be arranged and arranged, and a hole can be made in each medium for inoculation of mushroom spawn. For example, a hole may be formed along the circumferential surface of the medium, 2 to 12 holes may be formed, and mushroom spawn may be inoculated through each hole. Inoculation can be carried out manually or automatically, for example, the mushroom inoculation can be inoculated into each medium by an automatic inoculation machine (P13). When the inoculation is completed, growth conditions may be adjusted for cultivation of the seed bacteria according to the growth stage. Mycelium can be cultured from the seeded inoculation, and conditions for mycelial culture should be formed (P14). For mycelial culture, the seed germs will be maintained at a temperature of 20 to 30°C, preferably 23 to 27°C and a humidity of 60 to 80%, preferably a humidity of 68 to 72% for 10 to 30 days, preferably 15 to 20 days. You can. Under these conditions, when the hyphae grows to 4 to 10 cm, preferably 5 to 8 cm, holes of 1 to 5 cm can be formed in the range of 1 to 3 cm from the mycelium. As a result, the internal humidity or temperature may be controlled, thereby improving air contact with hyphae. Thus, after 10 to 30 days, preferably 15 to 20 days have elapsed since the primary circulation hole is formed, the secondary circulation hole of the same or similar size may be formed again. Thereafter, a humidification process for promoting growth of the mushroom peak may be performed (P15).

The humidification process may be performed after 50 to 90 days, preferably 50 to 80 days, after the hyphae is generated, but the timing may be appropriately selected according to the growth state of the mushroom and is not limited to the presented examples. For humidification, a number of media may be arranged vertically in a pre-made waterhole, or water may be supplied to the media frame in which the media is aligned. When multiple media are placed in a puddle, water can be supplied to the puddle to maintain the water level at 3 to 15 cm above the media while allowing the media to submerge completely for 1-3 hours. In this way, the temperature can be adjusted while the medium is submerged in water, and the temperature of the water can be adjusted to, for example, 24 to 32°C. Alternatively, each medium may be maintained under the conditions set forth above while preparing a pressure pump and supplying water to each medium so that the medium is completely immersed in water. And the top and bottom of the medium can be adjusted to be maintained at different temperatures depending on the layer, and growth conditions can be set to be different temperatures in the vertical direction. For example, while the temperature of the medium portion is 21 to 28°C, preferably 23 to 25°C, the temperature of the upper portion of the medium can be maintained at a temperature of 25 to 35°C, preferably 28 to 32°C. Alternatively, the temperature of the water and the temperature above the water can each be maintained at the temperatures indicated above. For example, while supplying water at a temperature of 1 to 2°C lower than the conditions given above, at the same time, the temperature difference in the vertical direction suggested above is formed while allowing air having a temperature of 1 to 5°C higher than the temperature given above to flow upwards of the water It can be done. The flow rate of air can be adjusted, and accordingly it can be maintained at the temperature indicated in the temperature of the medium immersed in water. As described above, different temperature conditions may be formed in the vertical direction while the medium is submerged in water, and the above-described state may be maintained for 1 to 5 hours, preferably 2 to 4 hours. Thereafter, the water is removed, and the medium can be kept in a state where air flow is possible. For example, the medium can be maintained at a temperature of 10 to 25° C. inside the mushroom house until the mushroom peak grows to a certain level. Specifically, while limiting the circulation of external air, the temperature of 5 to 12°C can be maintained for 2 to 6 hours, preferably 3 to 5 hours, per day, while maintaining the indoor temperature at 10 to 25°C. This ventilation period can be maintained until the mushroom peak reaches 1.0 to 2.0 cm, preferably 1.2 to 1.8 cm, most preferably 1.5 cm, and the packaging can be removed when the mushroom peak reaches the suggested size, eg For example, the plastic bag may be torn and removed. Along with this, mushroom peaks less than 1 cm or larger than 2 cm can be removed. Thereafter, a mushroom management step in which the mushroom hyphae becomes robust may be performed (P16). In the mushroom management step, the humidity of the mushroom medium can be maintained to be 60 to 70%, and the temperature can be maintained at 12 to 25°C. Thereafter, when the mushrooms grow in the size of 2 to 4 cm in diameter under the sunlight, the humidity may be adjusted to maintain the humidity at different times. While maintaining the bottom of the medium in a dry state, the humidity in the morning becomes 55 to 60%, and the humidity in the afternoon becomes 45 to 60%, so that the humidity in the afternoon can be kept low. After the mushrooms are fully grown, the medium can be completely opened and ventilated at 9 to 16 o'clock to remove moisture and maintain the humidity to be 65% or less, and also prevent moisture from entering by blocking the inflow of external air at night. Mushrooms can be harvested while allowing.

Ingredients and efficacy tests were performed on the mushrooms harvested by the cultivation method.

Ingredient test

Ingredient tests were conducted on the commercially available and commercially available white goods according to the present invention, and the contents of beta-glucan and amino acids were compared. Beta glucan was pre-treated by the Beta glucan assay Kit and analyzed using a spectrophotometer, and amino acids were pre-treated by a standard and buffer solution and analyzed using HPLC, and the test results Is as follows and amino acids were tested for constituent amino acids (essential amino acids).

Beta Clucan (%) Amino acid (mg%) Example 19.39 9,837.37 (4021.97) Control 15.92 8,612.78 (2,483.12)

Efficacy test

A sensory test was conducted on the white cultivars cultivated by the cultivation method according to the present invention and the commercially available white cultivars, and the sensory test comprises the diameter/thickness of the lampshade; Scent of mushrooms; And mushroom taste. For comparison, the diameter/thickness of the lamp was measured by selecting 100 randomly, and the aroma and taste were evaluated and averaged for 20 randomly selected 10. The flavor scent is the highest score of 10 points, and the lowest score of 1 point.

Example Control Diameter/thickness (cm) 6.2/1.6 5.3/1.2 incense 95 75 flavor 94 80

The cultivation method according to the present invention, as can be seen from the above table, was found to have a relatively rich and excellent sensory active ingredients. In addition, it was found that the probability of harvesting and becoming a product became more than 95%, and thus it had a relatively good productability.

The cultivation method of selenium-containing baekhwago according to the present invention is such that nutrients are absorbed into the human body during the ingestion process by injecting germanium or selenium complex with water into the medium to effectively absorb these nutrients in the growth process. The cultivation method according to the present invention makes it possible to harvest robust mushrooms while reducing the defective rate of mushroom cultivation. In addition, the cultivation method according to the present invention has the advantage of making the mushroom flesh softer while having a strong aroma and a large mushroom cluster.

The present invention has been described in detail with reference to the presented embodiments, but those skilled in the art will be able to make various modifications and modified inventions without departing from the technical spirit of the present invention with reference to the presented embodiments. . The present invention is not limited by such modified and modified inventions, but is limited by the appended claims.

Claims (3)

Forming a medium by mixing raw materials for inoculation of mushroom spawn;
Sterilizing the medium and inoculating;
Forming at least one hole in the medium according to the growth of the mycelia while culturing the seed bacteria;
Setting different temperature conditions according to vertical layers while humidifying the medium; And
A method of cultivating selenium-containing baekhwago, comprising removing the packaging of the medium and growing while controlling humidity. The method of claim 1, wherein the veggie is a cultivation method of selenium-containing baekhwago including sawdust-shaped crumbs, wheat bran, cottonseed shell, gypsum and selenium complex. The method of claim 1, wherein the humidification is a method of cultivating selenium-containing baekhwago, characterized in that while maintaining the water surface above the medium, the temperature of the upper layer forming different layers is maintained at a higher temperature than the lower layer.

Images