JPH05192036A - Culture of mushroom - Google Patents

Culture of mushroom

Info

Publication number
JPH05192036A
JPH05192036A JP4148921A JP14892192A JPH05192036A JP H05192036 A JPH05192036 A JP H05192036A JP 4148921 A JP4148921 A JP 4148921A JP 14892192 A JP14892192 A JP 14892192A JP H05192036 A JPH05192036 A JP H05192036A
Authority
JP
Japan
Prior art keywords
culture
medium
mushroom
fungus
mushroom fungus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP4148921A
Other languages
Japanese (ja)
Inventor
Genshiro Kawai
源四郎 川合
Yaichi Fukushima
弥一 福島
Kimiharu Okada
王春 岡田
Shiro Yamada
四郎 山田
Choji Fuse
長史 布施
Masamichi Osaki
勝通 大崎
Katsumi Yuasa
克己 湯浅
Hironaga Hashiba
弘長 橋場
Masaru Suzuki
勝 鈴木
Hiroshi Motai
宏 茂田井
Original Assignee
Kikkoman Corp
キッコーマン株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP16237891 priority Critical
Priority to JP3-162378 priority
Application filed by Kikkoman Corp, キッコーマン株式会社 filed Critical Kikkoman Corp
Priority to JP4148921A priority patent/JPH05192036A/en
Publication of JPH05192036A publication Critical patent/JPH05192036A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE:To maintain a liquid medium in a pulp-like state and efficiently culture a mushroom fungus having a strong rooting ability by inoculating and culturing the mushroom fungus in a solid medium, the mushroom fungus being produced by continuously culturing the fungus in a liquid medium diluted in a specific dilution rate and stirred with a specified stirring power. CONSTITUTION:A mushroom fungus continuously cultured in a liquid medium diluted in a dilution rate of <=0.05/h and stirred with a stirring power of >=0.4W/L is inoculated on a solid medium and cultured. A wood-rotting fungus such as Lentinus, Flammulina, Pleurotus, SHIROTAMOGITAKE, Pholiota nameko, Ganoderma, Coliolus, Tremella or Auricularia is especially effective as the utilized mushroom fungus. The conditions for the preliminary culture and the continuous culture of the mushroom fungus are somewhat different in dependence on the used strain, the composition of the employed medium, etc., and proper conditions are adopted in response to the mushroom fungus. Usually, the culturing temperature, the pH of the medium during the culture and the volume of aeration are approximately 20-40 deg.C, 3-8, and 0.1-2.0vvm, respectively. The culture adopts a perfectly mixing culture method e.g. with a stirring blades, aeration, etc.

Description

【発明の詳細な説明】Detailed Description of the Invention
【0001】[0001]
【産業上の利用分野】本願はキノコ菌を連続で液体培養
し、それを種菌として子実体を育成する発明に関する。
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an invention in which mushroom fungi are continuously liquid-cultured and used as seeds to grow fruiting bodies.
【0002】[0002]
【従来の技術】従来キノコ菌を液体培地で培養し子実体
を育成する例として「食用茸の栽培方法」(特開平2−
299516)、および「担子菌類の液状種菌の製造方
法」(特開平3−27218)を挙げることができる。
2. Description of the Related Art A conventional method for cultivating edible mushrooms has been described as an example of cultivating mushroom bodies in a liquid medium to grow fruiting bodies (JP-A-2-
299516), and "Method for producing liquid inoculum of basidiomycetes" (JP-A-3-27218).
【0003】まず前者は、担子菌類、同担子菌網におけ
る子実体の形成において、菌糸を増殖させる1次培養を
液中で行ない、子実体形成を菌床、あるいは原木栽培で
行なう食用キノコの栽培方法である。また後者は、担子
菌類を液体培地に静置培養した後、所要の細断処理を
し、細断された担子菌類を、細断菌類の乾燥抑制物質を
加えた滅菌水に混合する担子菌類の液状種菌の製造方法
である。
First, in the former, in the formation of fruiting bodies in basidiomycetes and the same basidiomycete net, primary culture for growing hyphae is carried out in a liquid, and fruiting body formation is carried out in a fungus bed or in raw wood cultivation. Is the way. In the latter, after statically culturing the basidiomycete in a liquid medium, the required shredding treatment is performed, and the shredded basidiomycete is mixed with sterilized water containing a substance for suppressing the drying of shredded fungi. It is a method for producing a liquid inoculum.
【0004】[0004]
【発明が解決しようとする課題】しかし前記従来例にお
いて開示されている内容は、回分式についてのみであ
り、培養の手段としてはあまり有効な方法とは言えな
い。すなわち回分式液体培養では、菌体が培地に均等に
分散しないいわゆるペレット状となってしまい、菌の活
着力すなわち培地に接種時の菌糸再生力が弱く、固体培
地における菌糸の蔓延速度が遅くなり、結果として多量
の菌体量を必要とし、また菌の生産性も悪い。さらに各
ロット毎に種菌を必要とし、かつ立上がりも時間がかか
りすぎる等種々欠点があった。
However, the contents disclosed in the above-mentioned conventional examples are only for the batch type and cannot be said to be a very effective method for culturing. That is, in batch liquid culture, the cells become so-called pellets that do not disperse evenly in the medium, and the viability of the cells, that is, the hypha regenerating ability at the time of inoculation into the medium is weak, and the spreading rate of the hypha in the solid medium becomes slow. As a result, a large amount of cells is required and the productivity of the cells is poor. Further, there are various drawbacks such as the fact that seeds are required for each lot and it takes too long to start up.
【0005】かかる現状に鑑み本願発明者は鋭意研究の
結果、液体培地の希釈率、および攪拌に要する動力をあ
る一定範囲に保持して連続培養すれば、液体培地がパル
プ状すなわち菌糸が均一に分散され菌体の活着力を強く
し、固体培地における菌糸の蔓延速度を上昇させること
が可能であり、かつ菌の生産性も向上させることができ
るできることを知見し本願発明を完成させた。すなわち
本願発明は、液体培地の希釈率が0.05/h以下、攪
拌動力0.4w/L以上で連続培養されたキノコ菌を、
固体培地に植菌し培養することを特徴とするキノコの栽
培方法である。
In view of the present situation, as a result of earnest research, the present inventor has found that if the liquid medium is continuously cultivated while keeping the dilution ratio and the power required for stirring within a certain range, the liquid medium becomes pulpy, that is, the mycelia are uniform. The inventors of the present invention have completed the present invention by discovering that they can be dispersed to strengthen the viability of the cells, increase the rate of hyphae spreading in a solid medium, and improve the productivity of the cells. That is, the invention of the present application is to provide a mushroom fungus continuously cultured at a liquid culture medium dilution rate of 0.05 / h or less and a stirring power of 0.4 w / L or more,
It is a mushroom cultivation method characterized by inoculating a solid medium and culturing.
【0006】[0006]
【課題を解決するための手段】本願発明において利用で
きるキノコ菌には限定はなく、どのようなキノコ菌でも
よいが、特にシイタケ、エノキタケ、ヒラタケ、シロタ
モギタケ、ナメコ、マンネンタケ、カワラタケ、シロキ
クラゲ、キクラゲ等木材腐朽菌に有効である。本願発明
で使用する液体培地としては、連続培養開始前のいわゆ
る前培養、および液体連続培養とも特に限定はなく、通
常のものでよい。その例として、可溶性澱粉、シューク
ロース、デキストリン、セルロース、グリセリン、醤油
油、フスマ等、窒素源としては例えば、ペプトン、肉エ
キス、酵母エキス、大豆粉、ヌカ、カゼイン、ポリペプ
トン、グルテン等、無機塩としたは例えば、各種リン酸
塩、硫酸塩、塩酸塩等が用いられ、さらに必要によりビ
タミン類、核酸等適宜加えた培地が用いられる。
The mushroom fungus that can be used in the present invention is not limited, and any mushroom fungus may be used, and in particular, shiitake mushroom, enokitake mushroom, oyster mushroom, shiitake moss mushroom, nameko, ganoderma lucidum, kawatake mushroom, sycamore, jellyfish Effective against wood decay fungi. The liquid medium used in the present invention is not particularly limited to so-called pre-culture before the start of continuous culture and liquid continuous culture, and may be an ordinary one. Examples thereof include soluble starch, sucrose, dextrin, cellulose, glycerin, soy sauce, fusuma, and the like.Examples of nitrogen sources include peptone, meat extract, yeast extract, soybean flour, casea, polypeptone, gluten, and inorganic salts. For example, various phosphates, sulfates, hydrochlorides and the like are used, and a medium to which vitamins, nucleic acid and the like are appropriately added if necessary is used.
【0007】キノコ菌の連続培養の条件であるが、希釈
率を0.05/h以下に、さらに攪拌機の動力を0.4
w/L以上に保持することが重要であり、この条件を満
たすことにより初めて液体培地をパルプ状に保つことが
可能で、活着力の強い菌を製造できるのである。すなわ
ち攪拌動力が小さすぎると、培地がパルプ状にならず、
また希釈率については、大きすぎると菌体濃度が下がる
からである。
The conditions for continuous cultivation of mushroom fungi are as follows: the dilution rate is 0.05 / h or less, and the power of the stirrer is 0.4.
It is important to maintain w / L or more, and it is possible to maintain the liquid medium in a pulp form only when this condition is satisfied, and it is possible to produce a bacterium having a strong vigor. That is, if the stirring power is too small, the medium does not become pulpy,
Also, with respect to the dilution rate, if it is too large, the bacterial cell concentration will decrease.
【0008】そして次にキノコ菌の前培養および連続培
養条件としては、使用する菌株、培地組成等により多少
異なり、菌に応じて適宜の培養条件を採用すればよい
が、通常培養温度は20〜40℃、培養中のpHは3〜
8、通気量は0.1〜2.0vvm程度であり、培養は
例えば攪拌翼、通気等による完全混合培養法を用いる。
そして前培養の期間は、使用する菌株の性質に応じて決
めればよいが、通常は24時間〜20日間位である。以
上のごとくしてキノコ菌を連続培養して得る培養液を直
接あるいは遠心分離等によって菌体を濃縮し、この菌を
固体培地に植菌し、キノコを栽培するのである。菌体は
パルプ状になっているので、菌糸の細断は必要無く、む
しろ活着力の低下を起こすので、細断は行わないほうが
良い。
[0008] Then, the pre-culture and continuous culture conditions of the mushroom fungus are slightly different depending on the strain to be used, the composition of the medium and the like, and appropriate culture conditions may be adopted depending on the fungus, but usually the culture temperature is 20 to 40 ° C, pH during culture is 3 ~
8. The aeration rate is about 0.1 to 2.0 vvm, and the culture is performed by a complete mixed culture method using, for example, a stirring blade and aeration.
The period of preculture may be determined depending on the properties of the strain to be used, but is usually about 24 hours to 20 days. As described above, mushrooms are cultivated by concentrating the bacterial cells directly or by centrifuging a culture solution obtained by continuously culturing mushroom fungi, and inoculating the fungi on a solid medium. Since the fungus body is in the form of pulp, it is not necessary to shred the mycelium because it does not require shredding of the hyphae and rather lowers the viability.
【0009】そして連続培養において、脂肪酸を前培養
後に培養槽へ供給する培地に添加することにより、キノ
コ菌の増殖、有用成分の生産に影響を与えることなく、
その抗菌作用により雑菌の繁殖を押さえることができ
る。特に連続液体培養装置においてキノコ菌を培養する
場合、液体培養貯槽に保存中の液体培地および培養槽に
連通する連結内の液体培地が雑菌の汚染を受けやすく、
またそこでの雑菌の増殖が著しいため、該部分の無菌化
に有効である
In continuous culture, by adding a fatty acid to a medium to be supplied to a culture tank after preculture, growth of mushroom fungi and production of useful components are not affected,
Due to its antibacterial effect, it is possible to suppress the growth of various bacteria. Especially when cultivating mushrooms in a continuous liquid culture device, the liquid medium being stored in the liquid culture storage tank and the liquid medium in the connection communicating with the culture tank are easily contaminated by miscellaneous bacteria,
Also, it is effective in sterilizing the part because the proliferation of various bacteria there is remarkable.
【0010】そして添加する脂肪酸またはその塩は、抗
菌作用、水溶性などの観点から炭素数が6以下、好まし
くは4以下の脂肪酸またはその塩が好適に利用できる。
脂肪酸またはその塩の培地への添加量は、前記液体培地
槽内の雑菌の汚染、増殖の防止、並びに連続培養時のキ
ノコ菌が抗菌作用を受けずに正常に培養されることのた
めに、0.01〜3.0%(W/V)の濃度となるよう
にすることが肝要である。すなわち、該添加量が濃度と
して3.0%(W/V)を越えるときは、キノコ菌の正
常な培養および有用物質の安定した生産ができなくなる
からである。
The fatty acid or salt thereof to be added is preferably a fatty acid or salt thereof having a carbon number of 6 or less, preferably 4 or less, from the viewpoint of antibacterial action, water solubility and the like.
The amount of fatty acid or a salt thereof added to the medium is for the contamination of miscellaneous bacteria in the liquid medium tank, the prevention of growth, and the fact that mushroom fungi during continuous culture are normally cultured without receiving an antibacterial action, It is important to set the concentration to 0.01 to 3.0% (W / V). That is, when the added amount exceeds 3.0% (W / V) as a concentration, normal cultivation of mushrooms and stable production of useful substances cannot be performed.
【0011】そして特に0.07〜2.0%(W/V)
の濃度となるようにするのが好適である。すなわち0.
07%(W/V)以上の場合には雑菌の防止がより完全
であるばかりでなく、液体回分培養ではキノコ菌の増
殖、有用物質の生産に阻害があるにもかかわらず、液体
連続培養ではそれがなく、一方2.0%以下の場合には
キノコ菌の極めて正常な培養および安定的な有用物質の
生産が行なわれるからである。なお脂肪酸またはその塩
を添加した培地(培地槽へ供給する以前の培地)のpH
は、6以下好ましくは5以下となるようにすることが、
雑菌の汚染防止の観点からさらに好ましい態様である。
And especially 0.07 to 2.0% (W / V)
It is preferable that the concentration is set to. That is, 0.
In the case of more than 07% (W / V), not only the prevention of various bacteria is more complete, but also the liquid batch culture inhibits the growth of mushroom fungi and the production of useful substances. On the other hand, if it is 2.0% or less, extremely normal culture of mushroom fungi and stable production of useful substances are carried out. The pH of the medium containing the fatty acid or its salt (the medium before being supplied to the medium tank)
Is 6 or less, preferably 5 or less,
This is a more preferable embodiment from the viewpoint of preventing contamination of various bacteria.
【0012】前記脂肪酸の具体例としては、例えば蟻
酸、プロピオン酸、酪酸、吉草酸、カプロン酸等が好適
なものとして挙げられ、中でも特に酢酸が好ましい。ま
た脂肪酸の塩としては、例えばアルカリ金属塩(ナトリ
ウム、カリウム等の塩)、アルカリ土類金属塩(カリウ
ム、マグネシウム等の塩)等である。そして該脂肪酸ま
たはその塩は、1種または2種以上組合せて用いること
ができる。
Specific examples of the fatty acid include formic acid, propionic acid, butyric acid, valeric acid, caproic acid and the like, and acetic acid is particularly preferable. Examples of the fatty acid salt include alkali metal salts (salts such as sodium and potassium) and alkaline earth metal salts (salts such as potassium and magnesium). The fatty acids or salts thereof can be used alone or in combination of two or more.
【0013】そしてさらに連続培養において、リグニン
を培養中に培養槽へ供給する培地に添加することによ
り、菌体濃度をより一層増加させることができる。リグ
ニンの培地への添加量は、極小量すなわち0.001〜
0.5%(W/V)程度で効果が得られるが、好適には
0.05〜0.2%(W/V)程度である。リグニンの
添加量は、前記量を超えて添加しても、効果は同じであ
る。リグニンとしては、ブナ、カシ、ナラ等広葉樹のリ
グニン、あるいはマツ、スギ、ヒノキ等針葉樹のリグニ
ンを挙げることができ、具体的には市販の脱アルカリリ
グニン、硫酸リグニン等、さらにはリグニンの構成モノ
マーであるバニリン、フェラル酸等を挙げることができ
る。
Further, in continuous culture, the cell concentration can be further increased by adding lignin to the medium supplied to the culture tank during the culture. The minimum amount of lignin added to the medium is 0.001-
The effect is obtained at about 0.5% (W / V), but it is preferably about 0.05 to 0.2% (W / V). Even if the amount of lignin added exceeds the above amount, the effect is the same. Examples of lignin include lignin of hardwood such as beech, oak, and oak, or lignin of conifer such as pine, cedar, and cypress. Specifically, commercially available dealkalized lignin, lignin sulfate, and the like, and further constituent monomers of lignin. Examples thereof include vanillin and ferric acid.
【0014】つぎに適宜選択された培地の殺菌方法はど
のような方法でもよい。例えば脂肪酸またはその塩を添
加した、またはしない培地を系外にて、予め回分式や連
続式で加熱殺菌あるいは膜濾過による除菌処理をしたの
ち、液体培地槽に注入し、脂肪酸またはその塩を添加し
ないときは、これを殺菌または除菌した培地に無菌的に
加えればよい。また脂肪酸またはその塩を添加した培地
を、液体培地貯槽内で殺菌するか、さらには連結部の適
当位置に設けた連続殺菌装置で殺菌する方法を用いても
よい。この後者の場合は、未殺菌の培地は脂肪酸または
その塩の添加によって雑菌の増殖が抑制されるので、そ
の殺菌も容易である。
Any sterilizing method for the medium selected appropriately may be used. For example, a medium to which a fatty acid or a salt thereof is added or not is subjected to a sterilization treatment by heat sterilization or membrane filtration in a batch system or a continuous system in advance, and then the liquid medium tank is filled with the fatty acid or a salt thereof. When it is not added, it may be added aseptically to a sterilized or sterilized medium. Further, a method of sterilizing a medium to which a fatty acid or a salt thereof is added in a liquid medium storage tank, or further using a continuous sterilizer provided at an appropriate position of the connecting portion may be used. In the latter case, the addition of a fatty acid or its salt suppresses the growth of various bacteria in the unsterilized medium, so that the sterilization is easy.
【0015】一方培養槽では、たとえば脂肪酸またはそ
の塩を含まない以外は前記培養貯槽内と同様の培地など
を用い、これに目的とするキノコ菌を接種して前培養を
行ない、菌が対数増殖期を経てある程度増殖した時期よ
り、前記培地貯槽の培地を連結部を介して培養槽へ連続
的に供給する。
On the other hand, in the culture tank, for example, the same medium as in the culture storage tank is used except that it does not contain fatty acids or salts thereof, and the target mushroom fungus is inoculated into this medium to carry out preculture, whereby the fungus grows logarithmically. After a certain period of growth, the medium in the medium storage tank is continuously supplied to the culture tank through the connecting portion.
【0016】次に液体連続培養によるキノコ菌を培養す
る固体培地としては、原木あるいはオガクズ、モミガラ
等の粒状物質にコメヌカ、フスマ等の栄養源を添加して
構成される菌床等が挙げられるが、培養の効率等の点か
ら後者の方が好ましい。
Next, examples of the solid medium for cultivating mushroom fungi by liquid continuous culture include a raw bed or granular material such as sawdust, rice husk, etc. and a bacterial bed formed by adding nutrient sources such as rice bran and bran. The latter is preferable from the viewpoint of culturing efficiency.
【0017】以下本願発明を具体的に説明するに、まず
ジャーファーメンターに培地を調製、殺菌、冷却後元種
菌を接種する。この元種菌は固体培養のものあるいは液
体培養のものでも良い。これを最初このまま通気培養
し、菌体濃度がある一定濃度例えば乾燥菌体量として
3.0mg/mL以上になったら、別に殺菌冷却してお
いた培地を一定の速度例えば培地希釈率D=0.01/
h以上で添加し、連続培養を開始する。培養期間中に連
続的あるいは断続的に一定間隔で培養液を取り出し、汚
染の有無をチェックし、菌体濃度の測定を行なう。菌体
濃度が徐々に増加し、所定の濃度例えば乾燥重量として
10mg/mL以上になったら、培養液を直接、あるい
は遠心機等を用いて菌体を濃縮後、あるいはこれを他の
気体・液体・粉体あるいはこれらの混合物と混合後、殺
菌調製済みの菌床あるいは原木等の固体培地に接種し、
適温にて培養しキノコ培養体を得る。ここで特に菌床培
養の場合接種部位を多くしたり、接種後菌床を混合する
とより早く菌床にキノコ菌の菌糸を蔓延させることが出
来る。
In order to explain the present invention in detail, first, a jar fermenter is inoculated with the original inoculum after preparing a medium, sterilizing and cooling. The original inoculum may be solid culture or liquid culture. This is first aerated and cultivated as it is, and when the cell concentration reaches a certain concentration, for example, 3.0 mg / mL or more as the dry cell amount, the medium that has been sterilized and cooled separately is kept at a certain rate, for example, the medium dilution rate D = 0. .01 /
Add more than h and start continuous culture. During the culturing period, the culture solution is taken out continuously or intermittently at regular intervals, and the presence or absence of contamination is checked and the bacterial cell concentration is measured. When the microbial cell concentration gradually increases and reaches a predetermined concentration, for example, 10 mg / mL or more as a dry weight, the culture medium is directly used, or the microbial cell is concentrated by using a centrifuge or the like, or this is mixed with another gas or liquid.・ After mixing with powder or a mixture of these, inoculate into a solid medium such as a sterilized bed or log.
Cultivate at a suitable temperature to obtain a mushroom culture. Here, particularly in the case of bacterial bed culture, if the inoculation site is increased or the bacterial bed is mixed after the inoculation, the hyphae of the mushroom fungus can be spread more quickly in the bacterial bed.
【0018】菌体の接種量は多ければ多いだけ菌の回り
が早いが、その分菌体の培養量を増やさなければならな
い。少なければそれだけ培養量は少なくて済むが、菌回
りに時間が掛り、またその間は汚染菌に対して抵抗力が
殆どないので汚染しやすい。そこでこれは培養室のクリ
ーン度等も考慮して決めなければならないが、一応の目
安としては、菌体の乾燥重量として0.1〜1.0g相
当の菌体を1kgの菌床に添加するのを標準とし、培養
環境や設備等を勘案してこれを前後させても良い。
The larger the amount of inoculum of the bacterial cells, the faster the rotation of the bacteria. However, the culture amount of the bacterial cells must be increased accordingly. If the amount is small, the amount of culture is small, but it takes a long time around the bacteria, and during that time, there is almost no resistance to contaminating bacteria, so that the bacteria are easily contaminated. Therefore, this must be determined in consideration of the cleanliness of the culture room, etc., but as a rough guideline, add 0.1 to 1.0 g of dry cells to 1 kg of bed. The above may be used as a standard, and this may be changed before or after considering the culture environment and equipment.
【0019】連続培養体を取り出してから培養基に接種
する迄の時間はなるべく短くすべきだが、もし保存が必
要な場合は低温(5℃以下)で7日以内が良い。連続液
体培養法によれば種菌は当然の事ながら連続で生産され
る。これに対して、キノコ培養基の生産の方は連続で行
なわない方がむしろ多い。夜間、休日や製造日程の都合
で種菌の接種を行なわない時には連続で生産された種菌
を保存しておいて、菌床の生産時にまとめて使用するこ
とも可能である。しかしこれが多量になったり長期に渡
るようになると、その保存設備が大変であり、またこれ
はより重要なことであるが、種菌としての活性の低下が
起こってしまう。
The time from the removal of the continuous culture to the inoculation of the culture medium should be as short as possible, but if it is necessary to store it, it is preferable to keep it at a low temperature (5 ° C. or lower) within 7 days. According to the continuous liquid culture method, the inoculum is naturally produced continuously. On the other hand, there are many cases where the mushroom culture medium is not continuously produced. It is also possible to store the continuously produced inoculum when the inoculum is not inoculated at night, on holidays or due to the production schedule, and then use the inoculum collectively at the time of producing the bacterial bed. However, if the amount of this becomes large or becomes long-term, its storage equipment becomes difficult, and more importantly, the activity as an inoculum decreases.
【0020】そこで本発明者らはこの点をも克服すべく
鋭意検討し、菌床の生産を行なわない時にはこの種菌の
液体連続培養を一時的に培地希釈率を低下または0にす
る方法を発明した。つまり、連続培養の培地希釈率を一
時的に低下または0にしても、培養体の種菌としての活
性が維持されるのである。また、この事により培地の節
約も計られ、これは技術的にまた経済的に非常に有利な
方法となった。ここで培地希釈率をどの程度低下させる
べきかは培地希釈率を変えた時の菌体量や種菌活性度等
を測定して求める必要があるが、一般的には半日程度の
極短期間ならばこれを0にしても種菌活性の低下が起き
ないことが多い。これ以上にわたる場合は個々の条件で
検討しなければならない。
Therefore, the inventors of the present invention have made diligent studies to overcome this point, and have invented a method of temporarily reducing the medium dilution rate of liquid continuous culture of this inoculum when the bacterial bed is not produced or reducing it to zero. did. In other words, the activity of the culture as an inoculum is maintained even if the medium dilution rate of continuous culture is temporarily reduced or even reduced to zero. This also saves medium, which is a very technically and economically advantageous method. Here, it is necessary to determine how much the medium dilution rate should be reduced by measuring the amount of cells and inoculum activity when the medium dilution rate is changed, but in general, if it is an extremely short period of about half a day, For example, even if this value is set to 0, the inoculum activity often does not decrease. If it exceeds this, it must be considered under individual conditions.
【0021】あるいはまた、培養槽内の培養物の取り出
し速度と培地添加速度を別々に制御する方法も有効であ
る。すなわち菌床の生産を行うときは、培養物の取り出
し速度を通常より早くすると、培養槽内の培養物の量が
減少してくる。そこで培地添加速度は培地希釈率をほぼ
一定に保つように低下させる。必要量の培養物を取り出
したら、この取り出しを停止する。これより培養槽内の
培養物の量は徐々に増加し元に戻る。この方法も非常に
有効である。
Alternatively, a method of separately controlling the removal rate of the culture medium from the culture tank and the medium addition rate is also effective. That is, when producing a bacterial bed, if the removal rate of the culture is made faster than usual, the amount of the culture in the culture tank will decrease. Therefore, the medium addition rate is reduced so that the medium dilution rate is kept almost constant. When the required amount of culture has been removed, this removal is stopped. From this, the amount of culture in the culture tank gradually increases and returns to the original level. This method is also very effective.
【0022】次にかくして得られたキノコ菌を固体培地
で培養するわけであるが、培養手段としては通常の手段
でよく、室温20〜25℃、湿度60〜65%にて70
〜100日程度培養し、次いで室温15℃、湿度90%
に変更して子実体発生の操作を行ないキノコを育成す
る。
The mushroom fungus thus obtained is then cultivated in a solid medium, and any ordinary means may be used as the culturing means, such as 70 at room temperature of 20 to 25 ° C. and humidity of 60 to 65%.
Culture for about 100 days, then room temperature 15 ℃, humidity 90%
Change to and operate the fruit body generation to raise mushrooms.
【0023】以下に実験例を示し、本願発明の効果を数
値的に示す。 実験例1 本実験例は、液体培地がパルプ状になる条件、並びにリ
グニンの効果を示すものである。 1.実験条件 (1)菌株;シイタケ菌(森465号) (2)培地組成 リグニンなしの場合 ポリプトン 0.4% イーストエキス 0.4% グルコース 5.0% リン酸1カリ 0.1% 硫酸マグネシウム 0.1% 塩酸カルシウム 0.1% リグニンを含む場合 ポリプトン 0.4% イーストエキス 0.4% グルコース 5.0% リン酸1カリ 0.1% 硫酸マグネシウム 0.1% 塩酸カルシウム 0.1% 脱アルカリリグニン 0.1% ※単位;W/V (3)培地pH;5.0 (4)培養温度;25℃ (5)通気量;0.5vvm (6)希釈率;0.02/h 2.実験装置;200Lジャファーメンタ。攪拌機は2
段ブレード式 ※前培養は、予め30日間坂口フラスコにて培養したも
の200mLを植菌し0.4w/Lで10日間培養し
た。その後連続培養をし、15日目のものを採取した。 ※攪拌動力の測定方法 (1)200Lジャーファーメンターに水を入れない
で、攪拌羽根を回しそれぞれの回転数での消費動力
(A)を求める。 (2)次に150Lの水を入れ、それぞれの回転数での
消費動力(B)を求める。 (3)攪拌動力を(B−A)/Vで求める。 3.実験結果 実験結果を表1に示す。 ×;ペレット状のもの ○;パルプ状ペレット状が混在するもの ◎;パルプ状のもの 以上の実験により攪拌動力が少なくとも0.4w/Lで
あれば、培地がパルプ状になることが証明される。ちな
みに0.4〜3.0w/Lにおいて、攪拌機の回転数は
100〜250rpmである。さらにリグニンを含む培
地の方が、菌体量が多いこともわかる。
Experimental examples are shown below to numerically show the effects of the present invention. Experimental Example 1 This experimental example shows the conditions under which the liquid medium becomes pulpy and the effect of lignin. 1. Experimental conditions (1) Strain; Shiitake bacterium (Mori 465) (2) Medium composition Without lignin Polypton 0.4% Yeast extract 0.4% Glucose 5.0% Phosphorus 1 potassium 0.1% Magnesium sulfate 0 1% Calcium Hydrochloride 0.1% Including Lignin Polyptone 0.4% Yeast Extract 0.4% Glucose 5.0% Phosphoric Acid 1 Potassium 0.1% Magnesium Sulfate 0.1% Calcium Hydrochloride 0.1% Desorption Alkaline lignin 0.1% * Unit: W / V (3) Medium pH; 5.0 (4) Culture temperature; 25 ° C (5) Aeration rate; 0.5 vvm (6) Dilution rate; 0.02 / h 2 . Experimental apparatus: 200L Jafermentor. 2 agitators
Step blade type * Pre-culture was carried out by inoculating 200 mL of the Sakaguchi flask that had been cultured for 30 days in advance and culturing at 0.4 w / L for 10 days. After that, continuous culture was carried out, and those on the 15th day were collected. * Measurement method of stirring power (1) Rotate the stirring blade without adding water to the 200L jar fermenter, and calculate the power consumption (A) at each rotation speed. (2) Next, add 150 L of water and determine the power consumption (B) at each rotation speed. (3) The stirring power is calculated as (BA) / V. 3. Experimental Results Experimental results are shown in Table 1. ×: Pellet-like ◯: Pulp-like pellets mixed ◎: Pulp-like From the above experiments, it is proved that the medium becomes pulp-like if the stirring power is at least 0.4 w / L. .. Incidentally, at 0.4 to 3.0 w / L, the rotation speed of the stirrer is 100 to 250 rpm. It can also be seen that the medium containing lignin has a higher amount of cells.
【0024】実験例2 実験例2に生産される菌体量および活着力について、本
願発明方法と従来方法との比較を示す。 A.本願発明方法 1.実験条件 実験例1に同じ。但し、攪拌動力は1.0w/Lとし
た。 2.実験装置 実験例1に同じ。 ※前培養も実験例1に同じで、連続培養後15日目のも
のを採取した。 B.従来方法(回分式) 1.実験条件 (1)菌株;シイタケ菌(森465号) (2)培地組成;実験例1に同じ。 (3)培養方法 予め30日かけて500mL容の坂口フラスコにて好気
的培養を行った。培養液200mLを種菌として、液量
150Lで200Lジャーファメンタで、下記条件にて
30日間培養を行った。 ・培養温度;25℃ ・培地pH;5.0 ・通気量;0.5vvm ・攪拌動力;1.0w/L 2.実験装置;200Lジャーファーメンター ※活着力の測定方法 オガ粉1Lに米糠0.1Lの割合で混合後、水分60%
に調整後、200Lの広口フラスコに50gづつ詰め1
18℃45分間飽和水蒸気にて加熱殺菌する。これを室
温まで冷却したものに、培養液1mLを加え、混合した
後、直径9cm厚さ9cmのシャーレに移す。このシャ
ーレを30℃で48時間培養し、培地表面に生じたシイ
タケ菌のコロニー数を活着力とする。 3.実験結果 実験結果を表2に示す。 本実験例により本願発明のほうが、従来方法によるもの
より菌の生産性および活着力とも優れていることが証明
される。
Experimental Example 2 With respect to the amount of cells and the viability of cells produced in Experimental Example 2, a comparison between the method of the present invention and the conventional method is shown. A. Method of the present invention 1. Experimental conditions Same as Experimental example 1. However, the stirring power was 1.0 w / L. 2. Experimental device Same as Experimental example 1. * The pre-culture was the same as in Experimental Example 1, and the sample 15 days after the continuous culture was collected. B. Conventional method (batch method) 1. Experimental conditions (1) Strain; Shiitake bacterium (Mori 465) (2) Medium composition: Same as Experimental Example 1. (3) Culture Method Aerobic culture was carried out in advance in a 500 mL Sakaguchi flask for 30 days. 200 mL of the culture solution was used as the inoculum, and the culture was performed for 30 days under the following conditions in a 200 L jar-famentor with a liquid volume of 150 L. -Culture temperature; 25 ° C-Media pH; 5.0-Aeration amount; 0.5 vvm-Agitation power; 1.0 w / L Experimental device: 200L jar fermenter * Method for measuring the vigor of mixing 60g of water after mixing 1L of Oga powder with 0.1L of rice bran
After adjusting to 1, pack 50g into a 200L wide-necked flask 1
Sterilize by heating with saturated steam at 18 ° C for 45 minutes. To this cooled to room temperature, 1 mL of the culture solution is added, mixed, and then transferred to a petri dish having a diameter of 9 cm and a thickness of 9 cm. This petri dish is cultured at 30 ° C. for 48 hours, and the number of colonies of Shiitake bacterium formed on the surface of the medium is used as the viability. 3. Experimental results Experimental results are shown in Table 2. This experimental example proves that the present invention is superior in productivity and viability of the bacterium to those of the conventional method.
【0025】[0025]
【実施例】【Example】
実施例1 シイタケ菌(森465号)をブドウ糖25g、ペプトン
4g、イーストエキス4g、燐酸1カリウム2g、硫酸
マグネシウム7水塩2g、水1000mLの組成よりな
る培地(培地A)100mLを500mL容坂口フラス
コに入れ、120℃、1気圧で15分間加熱蒸気殺菌し
室温まで放冷したものに植菌し、25℃で10日間振盪
培養する。このもの10本を種として1000L容ジャ
ーファーメンターに培地A800Lと東レシリコーン4
00mLを入れ120℃、1気圧で20分間加熱蒸気殺
菌し25℃まで冷却したものに植菌する。25℃で7日
間通気培養すると菌体濃度は徐々に増加し乾燥菌体重量
が3mg/mLとなった。ここで先と同じ組成の培地を
殺菌後冷却したものを、このジャーに培地希釈率D=
0.01/hの割合で添加し、連続培養を開始する。こ
れにより更に菌体濃度が増加し、7日後には乾燥菌体濃
度で10mg/mLを越えていた。連続培養は、25
℃、pH5、通気量0.5vvm、攪拌動力3.0w/
Lで行った。
Example 1 100 mL of a medium (medium A) having a composition of 25 g of Shiitake bacterium (Mori No. 465) in glucose, 4 g of peptone, 4 g of yeast extract, 2 g of potassium phosphate 1 g, 2 g of magnesium sulfate heptahydrate, and 1000 mL of water was added to a 500 mL Sakaguchi flask. After sterilizing by heating with steam at 120 ° C and 1 atm for 15 minutes and allowing to cool to room temperature, the cells are inoculated and cultured with shaking at 25 ° C for 10 days. Ten seeds of this product are used as seeds, and 1000 L jar fermenter is added to medium A 800 L and Toray Silicone 4
Inject 00 mL of the mixture in a sterilized mixture by heating and steam sterilization at 120 ° C. and 1 atm for 20 minutes and cooling to 25 ° C. When aeration culture was carried out at 25 ° C. for 7 days, the bacterial cell concentration gradually increased and the dry bacterial cell weight became 3 mg / mL. Here, the medium having the same composition as the above was sterilized and cooled, and the medium dilution ratio D =
Add at a rate of 0.01 / h to start continuous culture. As a result, the cell concentration further increased, and after 7 days, the dry cell concentration exceeded 10 mg / mL. Continuous culture is 25
C, pH 5, aeration rate 0.5 vvm, stirring power 3.0 w /
I went in L.
【0026】これ以降に得られた培養液を種菌として使
用したキノコ培養体を調製した。つまり、オガクズ1L
に生コメヌカ0.1Lの割合で混合後水分60%に調製
したものを通気口として多孔質フィルターを付けたポリ
プロピレン製の袋に1kg詰め、120℃で1時間加圧
加熱殺菌し、室温まで冷却したものに上記連続培養物3
0mLを加えて混合後、温度22℃、湿度60〜80%
の条件で80日間培養した。その後室温15℃、湿度9
0%に変更して子実体の発生操作を行ない、7日後に単
位培地当り160g/kgのシイタケを収穫し、以後2
0日目に70g/kg、40日目に80g/kgをそれ
ぞれ収穫した。
[0026] A mushroom culture was prepared using the culture broth obtained thereafter as an inoculum. That is, 1L of sawdust
1 kg of raw rice bran mixed with 0.1 L of raw rice bran was packed in a polypropylene bag equipped with a porous filter as a ventilation hole, sterilized by heating under pressure at 120 ° C for 1 hour, and cooled to room temperature. The above continuous culture 3
After adding 0 mL and mixing, the temperature is 22 ° C and the humidity is 60 to 80%.
The cells were cultured under the conditions of 80 days. Then room temperature 15 ℃, humidity 9
After changing to 0% and performing fruit body development operation, after 7 days, 160 g / kg of Shiitake mushroom per unit medium was harvested.
70 g / kg was harvested on day 0 and 80 g / kg was harvested on day 40.
【0027】実施例2 ナメコ(北研産業N217)をブドウ糖30g、ポリペ
プトン6g、イーストエキス4g、リン酸1カリウム1
g、硫酸マグネシウム7水塩0.5g、水1Lの組成の
培地(B)100mLを入れた500mL容坂口フラス
コにて、25℃で7日間振盪培養し、このもの10本を
1000L容ジャーファーメンターに培地B800Lと
東レシリコーン400mLを入れ120℃、1気圧で1
5分間加熱蒸気殺菌し25℃まで冷却したものに植菌す
る。25℃で5日間通気培養すると菌体濃度は徐々に増
加し乾燥菌体重量が3mg/mLとなった。ここで、別
に1000Lのジャーに先と同じ組成の培地に酢酸を
0.2%の濃度になるように添加し殺菌後冷却したもの
を、このジャーに培地希釈率D=0.02/hの割合で
添加し、連続培養を開始する。これにより更に菌体濃度
が増加し、5日後には乾燥菌体濃度で10mg/mLを
越えていた。連続培養は、25℃、pH5、通気量0.
5vvm、攪拌動力3.0w/Lで行った。
Example 2 30 g of Nameko (Hokuken Sangyo N217), 30 g of glucose, 6 g of polypeptone, 4 g of yeast extract, 1 potassium phosphate 1
g, 0.5 g of magnesium sulfate heptahydrate, and 100 mL of a medium (B) having a composition of 1 L of water were shake-cultured at 25 ° C. for 7 days in a 500-mL Sakaguchi flask. Add 800 L of culture medium B and 400 mL of Toray silicone to 120 ° C and 1 atm
Sterilize by heating with steam for 5 minutes and cool to 25 ° C to inoculate. When aeration culture was carried out at 25 ° C for 5 days, the bacterial cell concentration gradually increased and the dry bacterial cell weight became 3 mg / mL. Here, a 1000 L jar was added with acetic acid to a medium of the same composition as described above to a concentration of 0.2%, sterilized, and then cooled, and the medium dilution ratio D = 0.02 / h was added to this jar. Add at a rate to start continuous culture. As a result, the cell concentration was further increased, and after 5 days, the dry cell concentration exceeded 10 mg / mL. Continuous culture was performed at 25 ° C., pH 5, and aeration rate of 0.
It was carried out at 5 vvm and stirring power of 3.0 w / L.
【0028】このときの雑菌数および菌体量(乾物量)
を経時的に求めた。その結果を表3に示す。 本実施例において培養液中の雑菌数(個/mL)は、培
養液をガーゼで無菌的に濾過してナメタケ菌を除き、菌
数との関係で希釈倍率を考慮して生理植塩水で希釈し、
このうち1mLを肉エキス1%(W/V)、ポリペプト
ン1%(W/V)、酵母エキス0.5%、グルコース1
%、寒天1.5%、より成り、pH7.0の培地7mL
に混合し、これを37℃で24時間培養したのち、出現
したコロニー数を生菌数として測定した。
At this time, the number of miscellaneous bacteria and the amount of bacterial cells (dry matter amount)
Was determined over time. The results are shown in Table 3. In the present Example, the number of bacteria (cells / mL) in the culture broth was determined by sterilely filtering the culture broth with gauze to remove Nametake fungi, and diluting with physiological saline in consideration of the dilution ratio in relation to the number of bacteria. Then
Of this, 1 mL was 1% meat extract (W / V), 1% polypeptone (W / V), 0.5% yeast extract, 1 glucose.
%, Agar 1.5%, pH 7.0, 7 mL of medium
The mixture was mixed with, and the mixture was cultured at 37 ° C. for 24 hours, and the number of emerging colonies was measured as the viable cell count.
【0029】これ以降に得られた培養液を種菌として使
用したキノコ培養体を調製した。つまり、鋸粉1Lに生
米糠0.2Lの割合で混合後水分60%に調製したもの
を通気口として多孔質フィルターを付けたポリプロピレ
ン製の袋に 1kg詰め、120℃で1時間加圧加熱殺菌
し、室温まで冷却したものに上記連続培養物30mLを
上面より加えて混合せずにそのまま、温度22℃・湿度
60〜80%の条件で60日間培養した。その後室温1
5℃、湿度90%に変更して散水し、子実体の発生操作
を行ない、20日後に単位培地当り180g/kgのナ
メタケを収穫し、以後35日目に70g/kg、45日
目に50g/kgをそれぞれ収穫した。
Mushroom cultures were prepared using the culture broth obtained thereafter as seeds. In other words, 1 L of sawdust mixed with 0.2 L of raw rice bran and adjusted to 60% water content was packed in a polypropylene bag equipped with a porous filter as a vent, and 1 kg was packed and sterilized by heating under pressure at 120 ° C for 1 hour. Then, 30 mL of the above continuous culture was added to the one cooled to room temperature from the upper surface, and it was cultured as it was for 60 days under the conditions of a temperature of 22 ° C. and a humidity of 60 to 80% without mixing. Then room temperature 1
After changing the temperature to 5 ° C and humidity to 90%, watering was carried out, and the fruit body development operation was performed. After 20 days, 180 g / kg persimmon was harvested per unit medium, and 70 g / kg on the 35th day and 50 g on the 45th day thereafter. / Kg each was harvested.
【0030】実施例3 シイタケ菌(森465号)をブドウ糖25g、ペプトン
4g、イーストエキス4g、燐酸1カリウム2g、硫酸
マグネシウム7水塩2g、水1000mLの組成よりな
る培地(培地A)100mLを500mL容坂口フラス
コに入れ、120℃、1気圧で15分間加熱蒸気殺菌し
室温まで放冷したものに植菌し、25℃で10日間振盪
培養する。このもの10本を種として1000L容ジャ
ーファーメンターに培地A800Lと東レシリコーン4
00mLを入れ120℃、1気圧で20分間加熱蒸気殺
菌し25℃まで冷却したものに植菌する。25℃で7日
間通気培養すると菌体濃度は徐々に増加し乾燥菌体重量
が3mg/mLとなった。ここで、別に1000Lのジ
ャーに先と同じ組成の培地に脱アルカリリグニンを0.
1%の濃度になるように添加し殺菌後冷却したものを、
このジャーに培地希釈率D=0.01/hの割合で添加
し、連続培養を開始する。これにより更に菌体濃度が増
加し、7日後には乾燥菌体濃度で15mg/mLを越え
ていた。連続培養は、25℃、pH5、通気量0.5v
vmで、攪拌動力3.0w/L行った。
Example 3 100 mL of a medium (medium A) having a composition of 25 g of Ganoderma lucidum (Mori No. 465) of glucose, 4 g of peptone, 4 g of yeast extract, 1 g of potassium phosphate, 2 g of magnesium sulfate heptahydrate, and 1,000 mL of water (medium A) (500 mL) The mixture is placed in a Sakaguchi flask, sterilized by heating with steam at 120 ° C. and 1 atm for 15 minutes, allowed to cool to room temperature, inoculated, and shake-cultured at 25 ° C. for 10 days. Ten seeds of this product are used as seeds, and 1000 L jar fermenter is added to medium A 800 L and Toray Silicone 4
Inject 00 mL of the mixture in a sterilized mixture by heating and steam sterilization at 120 ° C. and 1 atm for 20 minutes and cooling to 25 ° C. When aeration culture was carried out at 25 ° C. for 7 days, the bacterial cell concentration gradually increased and the dry bacterial cell weight became 3 mg / mL. Here, separately, in a 1000 L jar, de-alkalized lignin was added to a medium having the same composition as the above.
What was added at a concentration of 1%, sterilized and cooled,
A medium dilution rate D = 0.01 / h is added to this jar to start continuous culture. As a result, the bacterial cell concentration further increased, and after 7 days, the dry bacterial cell concentration exceeded 15 mg / mL. Continuous culture is 25 ° C, pH 5, aeration rate 0.5v
The stirring power was 3.0 w / L at vm.
【0031】これ以降に得られた培養液を種菌として使
用したキノコ培養体を調製した。つまり、オガクズ1L
に生コメヌカ0.1Lの割合で混合後水分60%に調製
したものを通気口として多孔質フィルターを付けたポリ
プロピレン製の袋に1kg詰め、120℃で1時間加圧
加熱殺菌し、室温まで冷却したものに上記連続培養物3
0mLを加えて混合後、温度22℃、湿度60〜80%
の条件で80日間培養した。その後室温15℃、湿度9
0%に変更して子実体の発生操作を行ない、7日後に単
位培地当り160g/kgのシイタケを収穫し、以後2
0日目に70g/kg、40日目に80g/kgをそれ
ぞれ収穫した。
Mushroom cultures were prepared using the culture broth obtained thereafter as seeds. That is, 1L of sawdust
1 kg of raw rice bran mixed with 0.1 L of raw rice bran was packed in a polypropylene bag equipped with a porous filter as a ventilation hole, sterilized by heating under pressure at 120 ° C for 1 hour, and cooled to room temperature. The above continuous culture 3
After adding 0 mL and mixing, the temperature is 22 ° C and the humidity is 60 to 80%.
The cells were cultured under the conditions of 80 days. Then room temperature 15 ℃, humidity 9
After changing to 0% and performing fruit body development operation, after 7 days, 160 g / kg of Shiitake mushroom per unit medium was harvested.
70 g / kg was harvested on day 0 and 80 g / kg was harvested on day 40.
【0032】[0032]
【発明の効果】本願発明は以上のごとく構成されてお
り、活着力に優れたキノコ菌を効率よく生産することが
でき、キノコを効率よく栽培することが可能である。
EFFECTS OF THE INVENTION The present invention is constructed as described above, and it is possible to efficiently produce mushroom fungi having excellent vigor and to efficiently cultivate mushrooms.
フロントページの続き (72)発明者 山田 四郎 千葉県野田市野田339番地 キッコーマン 株式会社内 (72)発明者 布施 長史 千葉県野田市野田339番地 キッコーマン 株式会社内 (72)発明者 大崎 勝通 千葉県野田市野田339番地 キッコーマン 株式会社内 (72)発明者 湯浅 克己 千葉県野田市野田339番地 キッコーマン 株式会社内 (72)発明者 橋場 弘長 千葉県野田市野田339番地 キッコーマン 株式会社内 (72)発明者 鈴木 勝 千葉県野田市野田339番地 キッコーマン 株式会社内 (72)発明者 茂田井 宏 千葉県野田市野田339番地 キッコーマン 株式会社内Front page continued (72) Inventor Shiro Yamada, 339 Noda, Noda, Chiba Prefecture, Kikkoman Corporation (72) Inventor Nagashi Fuse, 339, Noda, Chiba Prefecture, Kikkoman Corporation (72) Inventor, Otsuka Katsutichi Chiba 339, Noda, Noda, Japan Kikkoman Corporation (72) Inventor, Katsumi Yuasa 339, Noda, Chiba, Noda City, Kikkoman Corporation (72) Inventor, Hironaga Hashiba, 339, Noda, Chiba Prefecture, Kikkoman Corporation (72) Invention Inventor Masaru Suzuki, 339 Noda, Noda, Chiba Prefecture, Kikkoman Corporation (72) Inventor, Hiroshi Motai, 339, Noda, Noda, Chiba Prefecture, Kikkoman Corporation

Claims (1)

    【特許請求の範囲】[Claims]
  1. 【請求項1】液体培地の希釈率が0.05/h以下、攪
    拌動力が0.4w/L以上で連続培養されたキノコ菌
    を、固体培地に接種し培養することを特徴とするキノコ
    の栽培方法。
    1. A mushroom characterized in that a solid medium is inoculated with a mushroom continuously cultivated at a dilution rate of a liquid medium of 0.05 / h or less and a stirring power of 0.4 w / L or more. Cultivation method.
JP4148921A 1991-06-07 1992-05-18 Culture of mushroom Pending JPH05192036A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP16237891 1991-06-07
JP3-162378 1991-06-07
JP4148921A JPH05192036A (en) 1991-06-07 1992-05-18 Culture of mushroom

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4148921A JPH05192036A (en) 1991-06-07 1992-05-18 Culture of mushroom

Publications (1)

Publication Number Publication Date
JPH05192036A true JPH05192036A (en) 1993-08-03

Family

ID=26478968

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4148921A Pending JPH05192036A (en) 1991-06-07 1992-05-18 Culture of mushroom

Country Status (1)

Country Link
JP (1) JPH05192036A (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009240275A (en) * 2008-03-31 2009-10-22 Naris Cosmetics Co Ltd Liquid culture medium for boletaceae suillus basidiomycete
JP2010200749A (en) * 2009-02-06 2010-09-16 Takara Bio Inc Method for producing liquid mushroom spawn
JP2011115153A (en) * 2009-10-27 2011-06-16 Takara Bio Inc Method for producing lyophyllum ulmarium fruiting body
CN102633552A (en) * 2012-04-26 2012-08-15 广东星河生物科技股份有限公司 Formula of culture medium for white beech mushroom and method for cultivating white beech mushroom
CN103493680A (en) * 2013-09-23 2014-01-08 重庆市中药研究院 Tremella liquid cultivar culture method and special culture medium for cultivar
CN103570424A (en) * 2013-11-25 2014-02-12 邬方成 Method for preparing white fungus cultivation material by utilizing production and processing waste of pecan
CN104106372A (en) * 2014-06-23 2014-10-22 黄艳芳 Lucid ganoderma mycelium and method for preparing lucid ganoderma mycelium body
CN105123268A (en) * 2015-08-31 2015-12-09 佛山市高明区更合镇鹏鹄食用菌专业合作社 High-yield tremella planting method
CN105766369A (en) * 2016-03-09 2016-07-20 湖北悟芝堂生物科技有限公司 Lucid ganoderma culturing system

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009240275A (en) * 2008-03-31 2009-10-22 Naris Cosmetics Co Ltd Liquid culture medium for boletaceae suillus basidiomycete
JP2010200749A (en) * 2009-02-06 2010-09-16 Takara Bio Inc Method for producing liquid mushroom spawn
JP2011115153A (en) * 2009-10-27 2011-06-16 Takara Bio Inc Method for producing lyophyllum ulmarium fruiting body
CN102633552A (en) * 2012-04-26 2012-08-15 广东星河生物科技股份有限公司 Formula of culture medium for white beech mushroom and method for cultivating white beech mushroom
CN103493680A (en) * 2013-09-23 2014-01-08 重庆市中药研究院 Tremella liquid cultivar culture method and special culture medium for cultivar
CN103570424A (en) * 2013-11-25 2014-02-12 邬方成 Method for preparing white fungus cultivation material by utilizing production and processing waste of pecan
CN103570424B (en) * 2013-11-25 2014-12-17 邬方成 Method for preparing white fungus cultivation material by utilizing production and processing waste of pecan
CN104106372A (en) * 2014-06-23 2014-10-22 黄艳芳 Lucid ganoderma mycelium and method for preparing lucid ganoderma mycelium body
CN105123268A (en) * 2015-08-31 2015-12-09 佛山市高明区更合镇鹏鹄食用菌专业合作社 High-yield tremella planting method
CN105766369A (en) * 2016-03-09 2016-07-20 湖北悟芝堂生物科技有限公司 Lucid ganoderma culturing system
CN105766369B (en) * 2016-03-09 2018-06-29 湖北悟芝堂生物科技有限公司 Ganoderma lucidum culture systems

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