CN112243780B - Method for culturing citrus plant cell sap with concentration regulation and control and without girdling - Google Patents

Method for culturing citrus plant cell sap with concentration regulation and control and without girdling Download PDF

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Publication number
CN112243780B
CN112243780B CN202011124906.6A CN202011124906A CN112243780B CN 112243780 B CN112243780 B CN 112243780B CN 202011124906 A CN202011124906 A CN 202011124906A CN 112243780 B CN112243780 B CN 112243780B
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fertilizer
parts
spraying
tips
foliar fertilizer
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CN112243780A (en
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阮经宙
陆玉英
阮俊翔
覃楠
吴承芳
龙娇月
杨雄娟
包亚萍
曾庆烨
黄建坤
罗玉珍
卢冬洁
李飞
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Guangxi Yuhualing Technology Development Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00—Methods for working soil
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/40—Treatment of liquids or slurries
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a citrus plant cell sap concentration regulation and control girdling-free cultivation method, which comprises the steps of cultivating spring tips each year to serve as fruiting branches and fruiting parent branches of the next year, applying Shi Chong fertilizer to roots 1-2 months each year, applying root system restoration fertilizer in the summer tip period within 10-20 days after flowers are removed each year, and spraying Shi Yuhua Ling No. 2 foliar fertilizer in the spring tip period so as to promote flowers, strengthen flowers and reduce the nutritional growth requirement of the spring tips; spraying Shi Yuhua Ling No. 2 foliar fertilizer in the summer shoot period to supplement nutrition to the tree body and reduce the nutrition growth requirement of the summer shoot; spraying Shi Yuhua Ling No. 2 foliar fertilizer in autumn and winter to promote flower bud differentiation in the next year and reduce nutrition growth requirements of autumn and winter; and (5) trimming useless tips of the grown spring tips, xia Shao, autumn tips and winter tips. According to the invention, through the combination of root Shi Chong fertilization, root system restoration fertilizer and foliar fertilizer spraying, the concentration of the cell sap of plants is improved, flower formation and fruit setting are facilitated, girdling is avoided, the consumption of manpower and material resources is reduced, and the loss caused by girdling is reduced.

Description

Method for culturing citrus plant cell sap with concentration regulation and control and without girdling
Technical Field
The invention relates to the technical field of citrus cultivation. More specifically, the invention relates to a cultivation method for regulating and controlling the cell sap concentration of citrus plants without girdling.
Background
The Citrus plants of the genus Rutaceae include Citrus reticulata, etc. The citrus investment return speed is high, and the characteristics of high economic benefit are widely favored by growers, and the continuous increase of market demands is also continuously stimulating the domestic citrus planting scale. The girdling is a common agricultural technique for citrus cultivation, and in the flowering period, the proper girdling of the branches can temporarily prevent organic matters from being conveyed downwards, increase nutrient accumulation at the upper part of a wound, promote flower bud differentiation and improve fruit quality; and (3) in the fruit retention period, the concentration of plant cell sap is increased by girdling, and flower and fruit dropping caused by overgrowth of overgrowth branches is inhibited. However, through years of production practice and interview of the orchard by the inventor, the annual girdling can cause excessive nutrition consumption of the tree body, the root system is weakened and dead, the tree vigor is reduced, and diseases and yield reduction are also caused.
The principle of the traditional girdling method is to block the downward transport channel of the overground part nutrition, promote the cell fluid concentration and the organic nutrition accumulation of overground part plants to realize the final purposes of flower bud differentiation, flower and fruit preservation and growth inhibition of the nutrition paste. In the whole process of girdling until the tree body is healed, the functions of nutrient absorption and moisture transmission are limited because the functions of the tree body are influenced, a series of changes of photosynthesis are caused, the self nutrition accumulation amount of the tree body is reduced, and the subsequent tree vigor decline and a series of adverse consequences are caused under the condition of lack of nutrition remedy by adding a large amount of nutrients consumed by flower bearing and wound healing; even if a series of nutrition measures are adopted for tree protection, sustainable stable yield and prolonged tree life are difficult to realize. On the other hand, the girdling operation belongs to one of agricultural operations with stronger professionals, the operation needs to be performed by adopting a specific cutter head and a specific method according to the concrete analysis of the tree situation, the girdling is deep, the damage to the tree is large, and the citrus yellowing and fading are easy to cause; the cutting is shallow, and the effect of flower and fruit preservation is poor. And not only is the manpower and material resources consumed, but also the loss caused by low operation repeatability to production is more difficult to estimate.
Disclosure of Invention
The invention starts with the essential reason that the citrus flower-promoting fruit-preserving period needs to be cut in a girdling way for many years, and the combination of root Shi Chong fertilization, root system restoration fertilizer and foliar fertilizer spraying promotes the cell sap concentration of plants, is beneficial to flower formation and fruit setting, reduces foliar-root water and nutrition circulation effect, slows down nutrition conveying speed, realizes girdling-free, reduces the consumption of manpower and material resources and reduces the loss caused by girdling.
To achieve these objects and other advantages and in accordance with the purpose of the invention, there is provided a citrus plant cell sap concentration control loop-free cultivation method comprising:
the method for retaining fruiting flower buds and retaining fruiting parent branches in the next year comprises the following steps: culturing spring tips each year as fruiting branches and fruiting parent branches of the next year, and wiping off excessive flower buds on the fruiting branches in the spring tip extraction period each year to leave fruiting flower buds; after the spring tips are planted, a plurality of flower-free spring tips are reserved and are cultivated into fruiting parent branches in the next year;
the fertilizing method comprises the steps of applying root fertilizer and spraying Shi Yuhua Ling No. 2 foliar fertilizer: the root fertilizer application is as follows: applying the root Shi Chong fertilizer 1-2 months each year, and applying the root system restoration fertilizer within 10-20 days after the flowers are withered each year in the summer tip period; the spray Shi Yuhua Ling No. 2 foliar fertilizer is: spraying Shi Yuhua Ling No. 2 foliar fertilizer in the spring tip stage to promote and strengthen flowers and reduce the nutrition growth requirement of spring tips; spraying Shi Yuhua Ling No. 2 foliar fertilizer in the summer shoot period to supplement nutrition to the tree body and reduce the nutrition growth requirement of the summer shoot; spraying Shi Yuhua Ling No. 2 foliar fertilizer in autumn and winter to promote flower bud differentiation in the next year and reduce nutrition growth requirements of autumn and winter;
the pruning method comprises the following steps: trimming useless tips of the grown spring tips, xia Shao tips, autumn tips and winter tips;
wherein, the fertilizing amount of the root system restoring fertilizer is calculated according to the yield: applying 1.0-3.5 kg of root repairing agent, 1.0-1.5 kg of waste honey liquid, 1.0-2.0 kg of peanut cake, 0.8-1.0 kg of potassium sulfate, 0.1-0.2 kg of lime powder, 0.01-0.15 kg of yeast extract and 0.1-0.8 kg of dipotassium hydrogen phosphate to each 60-100 kg of plant;
fertilizer application amount of the water flush fertilizer is calculated by yield: applying 2.5-5 kg of water flush fertilizer to each 60-100 kg of plants per yield; the water flush fertilizer is prepared from the following raw materials in parts by mass:
s1, mixing 20-40 parts of waste honey liquid, 5-15 parts of bean pulp, 6-13 parts of corn meal, 5-10 parts of fish meal, 5-10 parts of seaweed extract, 1-5 parts of rice bran and 20-40 parts of water;
s2, taking the mixture obtained in the step S1, regulating the pH value to 5.5-7.5 by using 1M dipotassium hydrogen phosphate or potassium dihydrogen phosphate buffer solution, uniformly mixing, and sterilizing at the high temperature of 120 ℃ for 30-70min to prepare a water flush fertilizer culture medium;
s3, mixing 30-55 parts of bacillus subtilis, 10-20 parts of saccharomycetes and 5-8 parts of bacillus amyloliquefaciens to obtain organic matter degrading bacterial powder;
s4, the mass ratio of the organic matter degrading bacteria powder to the water flush fertilizer culture medium is 1:50-100, stirring and fermenting at 25-30deg.C for 3-5 days;
s5, adding 10-12 parts of wheat bran, 30-45 parts of bean pulp powder and 5-7 parts of chitosan into the fermentation broth obtained in the step S4, and stirring and fermenting for 2-3 days at 40-50 ℃;
s6, mixing 0.5-1 part of neutral cellulase and 0.2-0.5 part of alkaline protease to form a degradation enzyme system, wherein the mass ratio of the degradation enzyme system to the fermentation liquor in the step S5 is (1): 50-100, and fermenting and decomposing for 7-10 days to obtain the water flush fertilizer.
Preferably, the thinning basis of reserving a plurality of flower-free spring tips is that 1-3 small branches are reserved according to the size of a basal branch, 1-4 thick branches are reserved, the rest are wiped off, and nutrition is supplied to reserved flower buds and reserved flower-free spring tips in a concentrated mode.
Preferably, the method of Shi Chong fertilization is to uniformly spread the water flush fertilizer under the tree crown, after fertilization, the fertilizer, weeds and dead branches and fallen leaves are turned over, part of citrus root systems are cut off and uniformly mixed in soil, the rotary tillage position is at least 0.1-0.8 m away from the base of the tree head, the turning over area is 20-50% of the orchard, the depth is 25-40 cm, and then the orchard is covered with the ground cloth.
Preferably, the method for applying the root system restoration fertilizer comprises uniformly spreading the root system restoration fertilizer under the tree crown, turning over the fertilizer, weeds and dead branches and fallen leaves after fertilization, cutting off part of citrus root systems, uniformly mixing in soil, and covering the root systems with the whole field of land cloth, wherein the rotary tillage position is at least 0.1-0.8 m away from the base of the tree head, the rotary tillage area is 20-50% of the orchard, and the depth is 25-40 cm.
Preferably, the root system repairing agent is prepared from the following raw materials in parts by mass: 89 parts of waste honey liquid, 8 parts of goat horn ash, 0.4 part of saccharomycetes and 0.2 part of ferment bacteria, wherein the goat horn ash is powder prepared by soaking, drying, burning and crushing waste goat horns; the Yuhualiing No. 2 foliar fertilizer comprises the following components in parts by mass: 94 parts of waste honey liquid, 3 parts of amino acid, 0.8 part of choline chloride, 0.4 part of saccharomycetes, 0.2 part of ferment bacteria, 0.8 part of urea, 0.5 part of ferrous sulfate, 0.2 part of boric acid, 0.05 part of ammonium molybdate, 6 parts of claw beam ash and 10 parts of glycerin, wherein the Yuhualing No. 2 foliar fertilizer is diluted 180-200 times when being sprayed.
Preferably, spraying Shi Yuhua Ling No. 2 foliar fertilizer in the spring tip stage comprises spraying Yuhualiling No. 2 foliar fertilizer when the peanut bud is in the spring tip stage and before the bud is smeared, and spraying Yuhualiling No. 2 foliar fertilizer in the first and second physiological fruit dropping stages;
spraying the Yuhualiing No. 2 foliar fertilizer when the peanut buds are in spring tip stage and before the buds are smeared, specifically comprises the following steps: under the condition of extremely small or no flower quantity, spraying the Yuhualiling 2 foliar fertilizer mixed solution of Yuhualiling 2 foliar fertilizer and ethephon foliar fertilizer once when the spring tips are 1-3 cm long, wherein the concentration of ethylene Li Zaisuo in the Yuhualiling 2 foliar fertilizer mixed solution is lower than 200ppm, and spraying the Yuhualiling 2 foliar fertilizer once again 5-7 days after spraying;
spraying the Yuhualiing No. 2 foliar fertilizer in the first and second physiological fruit dropping periods specifically comprises the following steps: under the condition of large flower quantity, spraying the Yuhualing No. 2 foliar fertilizer once when young spring tip leaves are unfolded; under the condition of insufficient flower quantity, when spring tips grow 1-3 cm, spraying the Yuhualiing No. 2 foliar fertilizer once;
the leaf fertilizer for Shi Yuhua Ling No. 2 sprayed in the summer tip period comprises the following specific components: when flowers are reduced to 80-85%, spraying the Yuhualiing No. 2 foliar fertilizer once, if the nutrition of the fruit tree is insufficient, the summer shoots are early in growth, and when the summer shoots are exposed to white, spraying the Yuhualiing No. 2 foliar fertilizer once;
the Shi Yuhua Ling No. 2 foliar fertilizer sprayed in autumn and winter is specifically: continuously spraying the Yuhualiing No. 2 foliar fertilizer twice in the late autumn and early winter, and spraying the foliar fertilizer for the second time at intervals of 7-10 days; if the temperature is high in winter, or the rainwater is too high, or the tree vigor is more vigorous, the mixed liquid of the Yuhualiling 2 foliar fertilizer added with the paclobutrazol is sprayed once, and the concentration of the paclobutrazol in the mixed liquid of the Yuhualiling 2 foliar fertilizer is 500ppm, so that the flower bud differentiation of the next year is promoted.
Preferably, the pruning target of the pruning method is that after pruning, the young shoots are not extracted, so that strong light is prevented from being directly irradiated by fruits, and the bore branches are kept under care and sunlight is absorbed; the time selection of the pruning method is based on that the trees planted in the relatively weak or dry land with more results are pruned early, the trees planted in the paddy field with less results are pruned late; the pruning method has the following pruning standards for branch tips: the main branch tips of the result are reserved, the rest of pest branches, weak branches, residual branches after fruiting and excessive branches are cut off, the tree body is ventilated and transparent, and the result is that the Xia Shao, autumn tips and winter tips which are extracted and generated on the main branch are properly cut off or cut off according to the size and height of the crown.
Preferably, the pruning method further comprises quantifying the reserved tips, wherein the quantified reserved tips are as follows: setting a tree with the yield of 30-40 kg for the fruit tree, and keeping 30-50 branches of the fruiting parent branches; setting a tree with the yield of 40-60 kg for the fruit tree, and keeping 50-80 branches of parent branches as a result; the yield of the fruit tree is set to be 60-110 kg, and the number of the reserved parent branches is 70-100.
The invention at least comprises the following beneficial effects:
1. by combining root Shi Chong fertilization, root system restoration fertilizer and foliar fertilizer spraying, the concentration of the cell sap of plants is improved, flower formation and fruit setting are facilitated, the water and nutrition circulation effect of leaf surfaces and root systems is reduced, the nutrition conveying speed is slowed down, girdling is avoided, the consumption of manpower and material resources is reduced, and the loss caused by girdling is reduced.
1.1, bacillus subtilis, saccharomycetes and bacillus amyloliquefaciens have the characteristic of being capable of rapidly dividing and proliferating in soil and matrixes under the condition of sufficient nutrition, under the premise of nutrition induction, dominant bacterial groups can be rapidly formed in the soil along with fertilizer application, and after the bacterial groups are planted in a fixed mode, plant remains such as rich branches and leaves in the soil environment, senescent roots and the like can be decomposed into organic components such as humic acid, fulvic acid and the like through fermentation, and the organic components can be directly absorbed by plant roots to supplement the concentration of citrus plant cell sap.
1.2, the micro-molecular carbon sources such as oligosaccharide, polysaccharide, oligopeptide, amino acid and the like in the waste honey liquid and the biological nitrogen sources such as the amino acid, oligopeptide and the like in the fish meal and the corn meal can be rapidly utilized by the flora, so that the flora can be rapidly proliferated, the number of viable bacteria can be increased in a short time, and a foundation is laid for replacing harmful flora in soil after flushing and applying and constructing humic circulation flora. Meanwhile, the nutrient structure which takes crude fiber as main or unique carbon source in the middle and later stages of fermentation is constructed by adding cellulose-rich and polysaccharide-rich agricultural grain production waste materials such as soybean meal, rice bran and the like into a culture medium, and the change of the nutrient structure of the flora can be induced after the consumption of the micromolecular carbon source is over and the flora quantity is increased, so that the metabolic capacity of degrading cellulose into micromolecular sugar and humic acid is increased.
1.3, after entering the deep fermentation process, the flora subjected to nutrition screening is suitable for the condition of taking crude fiber as a main carbon source, bean pulp powder and wheat bran are further added for deep fermentation to generate a large amount of humic acid components, so that the nutrition components of the water flush fertilizer and the capability of improving the concentration of plant cell sap are improved; on the other hand, the amino-oligosaccharide components generated in the fermentation process of chitosan and algal polysaccharide can be absorbed by citrus root systems along with the water flush fertilizer, so that the nascent root systems are induced to generate, and the absorption effect of the nutrient paste is improved.
1.4, adding degrading enzyme system to ferment until the end to carry out enzymolysis on the macromolecule components such as cellulose, crude protein and the like remained in the fermentation liquid, thoroughly converting the macromolecule components into components such as humic acid, amino acid, micromolecular sugar, oligopeptide and the like which can be absorbed and utilized by plants, and further improving the nutrition components and absorption effect of the water flush fertilizer.
2. The rotary tillage breaks partial old roots, so that the water absorption capacity is reduced, the cell sap is not easy to dilute, the nutrition absorption is accelerated under the growth of explosive new root tips, the annual high concentration of plant cell sap is maintained, and the ring cutting is further facilitated.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is described in further detail below to enable those skilled in the art to practice the invention by reference to the specification.
It will be understood that terms, such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1 ]
A cultivation method for regulating and controlling cell sap concentration of citrus plants without girdling comprises the following steps:
1) The method for retaining fruiting flower buds and retaining fruiting parent branches in the next year comprises the following steps: culturing spring tips each year as fruiting branches and fruiting parent branches of the next year, and wiping off excessive flower buds on the fruiting parent branches in the spring tip extraction period each year to leave fruiting flower buds; after the spring tips are planted, a plurality of flower-free spring tips are reserved and are cultivated into fruiting parent branches in the next year;
1.1 The thinning basis of the reserved plurality of flower-free spring tips is that 1-3 small branches are reserved according to the size of a basal branch, 1-4 thick branches are reserved, the rest branches are wiped off, and nutrition is supplied to reserved flower buds and reserved flower-free spring tips in a concentrated mode.
2) The fertilizing method comprises the steps of applying root fertilizer and spraying Shi Yuhua Ling No. 2 foliar fertilizer: the root fertilizer application is as follows: applying the root Shi Chong fertilizer 1-2 months each year, and applying the root system restoration fertilizer within 10-20 days after the flowers are withered each year in the summer tip period; the spray Shi Yuhua Ling No. 2 foliar fertilizer is: spraying Shi Yuhua Ling No. 2 foliar fertilizer in the spring tip stage to promote and strengthen flowers and reduce the nutrition growth requirement of spring tips; spraying Shi Yuhua Ling No. 2 foliar fertilizer in the summer shoot period to supplement nutrition to the tree body and reduce the nutrition growth requirement of the summer shoot; spraying Shi Yuhua Ling No. 2 foliar fertilizer in autumn and winter to promote flower bud differentiation in the next year and reduce nutrition growth requirements of autumn and winter;
2.1 Fertilizer application amount of root system restoration fertilizer to yield fertilizer: applying 1.0 kg of root repairing agent, 1.0 kg of waste honey solution, 1.0 kg of peanut cake, 0.8 kg of potassium sulfate, 0.1 kg of lime powder, 0.01 kg of yeast extract and 0.1 kg of dipotassium hydrogen phosphate to 60 kg of plants per yield;
fertilizer application amount of the water flush fertilizer is calculated by yield: applying 2.5 kg of water flush fertilizer to each 60 kg of plants; the water flush fertilizer is prepared from the following raw materials in parts by mass:
s1, mixing 20 parts of waste honey liquid, 5 parts of soybean meal, 6 parts of corn meal, 5 parts of fish meal, 5 parts of seaweed extract, 1 part of rice bran and 20 parts of water;
s2, taking the mixture obtained in the step S1, regulating the pH value to 6 by using 1M dipotassium hydrogen phosphate or potassium dihydrogen phosphate buffer solution, uniformly mixing, and sterilizing at the high temperature of 120 ℃ for 30min to prepare a water flush fertilizer culture medium;
s3, mixing 30 parts of bacillus subtilis, 10 parts of saccharomycetes and 5 parts of bacillus amyloliquefaciens to obtain organic matter degrading bacterial powder;
s4, the mass ratio of the organic matter degrading bacteria powder to the water flush fertilizer culture medium is 1:50, mixing, stirring and fermenting at 25-30deg.C for 3 days;
s5, adding 10 parts of wheat bran, 30 parts of bean pulp powder and 5 parts of chitosan into the fermentation broth obtained in the step S4, and stirring and fermenting for 2 days at 40-50 ℃;
s6, mixing 0.5 part of neutral cellulase and 0.2 part of alkaline protease to form a degradation enzyme system, wherein the mass ratio of the degradation enzyme system to the fermentation liquor in the step S5 is 1:50, mixing, fermenting and decomposing for 7 days to prepare the water flush fertilizer.
2.2 The Shi Chong fertilizer and the root system repairing fertilizer are all characterized in that the fertilizer is uniformly scattered under the tree crowns, after the fertilizer is applied, the fertilizer, weeds and dead branches are turned over, part of citrus root systems are cut off and uniformly mixed in soil, the rotary tillage position is 0.8 m away from the base of the tree head, the turning over area is 20% of the orchard, the depth is 25cm, and then the orchard is covered with the land cloth.
2.3 The root system repairing agent is a root system repairing agent of patent number ZL 201510439687.3, and is specifically prepared from the following raw materials in parts by mass: 89 parts of waste honey liquid, 8 parts of goat horn ash, 0.4 part of saccharomycetes and 0.2 part of ferment bacteria, wherein the goat horn ash is powder prepared by soaking, drying, burning and crushing waste goat horns. The Yuhualiing No. 2 foliar fertilizer is a foliar fertilizer of patent number ZL201510439697.7, and specifically comprises the following components in parts by mass: 94 parts of waste honey liquid, 3 parts of amino acid, 0.8 part of choline chloride, 0.4 part of saccharomycetes, 0.2 part of ferment bacteria, 0.8 part of urea, 6 parts of claw beam ash and 10 parts of glycerin, wherein the Yuhualiing No. 2 foliar fertilizer is diluted by 200 times when being sprayed.
2.4 Spraying Shi Yuhua Ling No. 2 foliar fertilizer in the spring tip stage comprises spraying Yuhualiling No. 2 foliar fertilizer during the peanut bud in the spring tip stage and before the bud is smeared, and spraying Yuhualiling No. 2 foliar fertilizer in the first and second physiological fruit dropping stages;
2.41 Spraying the Yuhualiing No. 2 foliar fertilizer during the peanut bud picking in the spring tip period and before the bud picking, specifically comprises the following steps: under the condition of little or no flower quantity, spraying the Yuhualiling No. 2 foliar fertilizer once when the spring tips are 1-3 cm long, and spraying the Yuhualiling No. 2 foliar fertilizer once again 5-7 days after spraying;
2.42 The spraying of the Yuhualiing No. 2 foliar fertilizer in the first and second physiological fruit dropping periods is specifically as follows: under the condition of large flower quantity, spraying the Yuhualing No. 2 foliar fertilizer once when young spring tip leaves are unfolded; under the condition of insufficient flower quantity, when spring tips grow 1-3 cm, spraying the Yuhualiing No. 2 foliar fertilizer once.
2.5 The spraying of Shi Yuhua Ling No. 2 foliar fertilizer in the summer tip period specifically comprises: when flowers are reduced to 80-85%, the Yuhualiing No. 2 foliar fertilizer is sprayed once, if the nutrition of the fruit tree is insufficient, the summer shoots are early in growth, and when the summer shoots are exposed to white, the Yuhualiing No. 2 foliar fertilizer can be sprayed once.
2.6 The Shi Yuhua Ling No. 2 foliar fertilizer sprayed in autumn and winter is specifically: continuously spraying the Yuhualiing No. 2 foliar fertilizer twice in the late autumn and early winter, and spraying the foliar fertilizer for the second time at intervals of 7-10 days; if the temperature is high in winter, or the rainwater is too high, or the tree vigor is more vigorous, the Yuhualiing No. 2 foliar fertilizer is sprayed once to promote the flower bud differentiation in the next year.
3) The pruning method comprises the following steps: and (5) trimming useless tips of the grown spring tips, xia Shao, autumn tips and winter tips.
3.1 The pruning target of the pruning method is that after pruning, the young shoots are not pulled out, so that strong light is prevented from direct irradiation by fruits, and the bore branches are kept under care and sunlight is absorbed; the time selection of the pruning method is based on that the trees planted in the relatively weak or dry land with more results are pruned early, the trees planted in the paddy field with less results are pruned late; the pruning method has the following pruning standards for branch tips: the main branch tips of the result are reserved, the rest of pest branches, weak branches, residual branches after fruiting and excessive branches are cut off, the tree body is ventilated and transparent, and the result is that the Xia Shao, autumn tips and winter tips which are extracted and generated on the main branch are properly cut off or cut off according to the size and height of the crown.
3.2 The trimming method further comprises quantifying the retained tips, wherein the quantified retained tips are as follows: setting a tree with the yield of 30-40 kg for the fruit tree, and keeping 30-50 branches of the fruiting parent branches; setting a tree with the yield of 40-60 kg for the fruit tree, and keeping 50-80 branches of parent branches as a result; the yield of the fruit tree is set to be 60-110 kg, and the number of the reserved parent branches is 70-100.
Example 2 ]
The method for culturing citrus plant cell sap concentration regulation and control without girdling is shown in the embodiment 1, wherein the difference is that the raw materials of the water flush fertilizer are different in mass parts, and the other steps are the same as the embodiment 1, and the difference is specifically that the fertilizing amount of the water flush fertilizer is calculated according to fertilizer production: applying 2.5 kg of water flush fertilizer to each 60 kg of plants; the water flush fertilizer is prepared from the following raw materials in parts by mass:
s1, mixing 40 parts of waste honey liquid, 15 parts of soybean meal, 13 parts of corn meal, 10 parts of fish meal, 10 parts of seaweed extract, 5 parts of rice bran and 40 parts of water;
s2, taking the mixture obtained in the step S1, regulating the pH value to 6 by using 1M dipotassium hydrogen phosphate or potassium dihydrogen phosphate buffer solution, uniformly mixing, and sterilizing at the high temperature of 120 ℃ for 70min to prepare a water flush fertilizer culture medium;
s3, mixing 55 parts of bacillus subtilis, 20 parts of saccharomycetes and 8 parts of bacillus amyloliquefaciens to obtain organic matter degrading bacterial powder;
s4, the mass ratio of the organic matter degrading bacteria powder to the water flush fertilizer culture medium is 1:100, mixing, stirring and fermenting at 25-30deg.C for 5 days;
s5, adding 12 parts of wheat bran, 45 parts of bean pulp powder and 7 parts of chitosan into the fermentation broth obtained in the step S4, and stirring and fermenting for 3 days at 40-50 ℃;
s6, mixing 1 part of neutral cellulase and 0.5 part of alkaline protease to form a degradation enzyme system, wherein the mass ratio of the degradation enzyme system to the fermentation broth in the step S5 is 1:100, mixing, fermenting and decomposing for 10 days to prepare the water flush fertilizer.
Example 3 ]
The cultivation method for regulating and controlling the cell sap concentration of the citrus plant without girdling is shown in the embodiment 1, wherein the difference is that the root fertilizer application modes are different, and the root system restoration fertilizer application and the water flush fertilizer application in the embodiment 3 are as follows: an annular ditch with the depth and width of 15-30cm is dug at the periphery of the crown of the tree with the depth and width of 10-25cm, the root system restoration fertilizer is applied into the ditch, covered with soil, watered and drip-irrigated according to the requirement, and the other steps are the same as those of the embodiment 1.
Comparative example 1 ]
A citrus cultivation method is shown in example 1, wherein the raw materials of the water flush fertilizer are different, and the water flush fertilizer of comparative example 1 does not include waste honey solution, and the other materials are the same as in example 1.
Comparative example 2 ]
A citrus cultivation process is shown in example 1, wherein the raw materials of the flush fertilizer are different, specifically the flush fertilizer step S1 of comparative example 2 does not include fish meal and corn meal, and otherwise is the same as in example 1.
Comparative example 3 ]
A citrus cultivation method is shown in example 1, wherein the raw materials of the water flush fertilizer are different, and the water flush fertilizer step S1 of comparative example 3 is different from the soybean meal and rice bran, and the other steps are the same as in example 1.
Comparative example 4 ]
A citrus cultivation method is shown in example 1, wherein the raw materials of the water flush fertilizer are different, and the water flush fertilizer step S5 of comparative example 4 is different from the bean pulp powder and wheat bran, and the other steps are the same as in example 1.
Comparative example 5 ]
A citrus cultivation method is shown in example 1, wherein the raw materials of the water flush fertilizer are different, and the water flush fertilizer of comparative example 5 does not include chitosan and seaweed extract, and the other materials are the same as in example 1.
Comparative example 6 ]
A citrus cultivation method is shown in example 1, except that the root system restoration fertilizer of comparative example 6 is different in raw material, and the root system restoration fertilizer does not include waste honey solution, otherwise the same as in example 1.
Comparative example 7 ]
A citrus cultivation method is shown in example 1, wherein the raw materials and the preparation method of the water flush fertilizer are different, and the other water flush fertilizer is the same as example 1, specifically the water flush fertilizer of comparative example 7 comprises, by mass, 25 parts of commercial humic acid, 10 parts of commercial fulvic acid, 20 parts of waste honey solution, 5 parts of soybean meal, 6 parts of corn meal, 5 parts of fish meal, 5 parts of seaweed extract and 1 part of rice bran, and a second mixture of 10 parts of wheat bran, 30 parts of soybean meal and 5 parts of chitosan, and an organic matter degrading bacterial powder prepared by mixing 30 parts of bacillus subtilis, 10 parts of saccharomycetes and 5 parts of bacillus amyloliquefaciens, a degrading enzyme system prepared by mixing 0.5 part of neutral cellulase and 0.2 parts of alkaline protease, and stirring and fermenting for 3 days at 25-30 ℃, wherein the mass ratio of the organic matter degrading bacterial powder to the first mixture is 1:50, and the mass ratio of the degrading enzyme system to the first mixture and the second mixture is 1:50.
Comparative experiment 1 of the citrus cultivation methods of examples 1 to 3 and comparative examples 1 to 7-
Experiments are carried out on seedless citrus which enters a full fruit period after 4.5 years of planting in a certain producing area, the water content, fertility, pH value and topography of the soil of the experimental land are the same, the planting period and tree vigor are similar, the planting period and tree vigor are randomly divided into 10 groups, each group of examples 1-3 occupies 1 mu of land, each group of comparative examples 1-7 occupies 0.5 mu of land, and 100-107 seedless citrus plants are planted per mu. Each group was cultivated and managed according to the cultivation methods of the corresponding examples 1 to 3 and comparative examples 1 to 7. The economic indexes such as average girdling rate per mu in bud picking and flowering period, average girdling rate per mu in summer tip period, average girdling rate per mu in autumn tip period, average fruiting rate per mu, average single plant yield and reduced mu yield are measured, and the statistics are shown in table 1.
Table 1:
Figure BDA0002733274480000091
Figure BDA0002733274480000101
comparative experiment 2 of the citrus cultivation methods of examples 1 to 3 and comparative examples 1 to 7 ]
The experiment is carried out on the sugar oranges planted in a certain producing area for 5 years and entering the full fruiting period, the water content, fertility, pH value and topography of the soil of the experimental land are the same, the planting period and tree vigor are similar, the soil is randomly divided into 10 groups, each group of examples 1-3 occupies 1 mu, each group of comparative examples 1-7 occupies 0.5 mu, and 90-100 strains of sugar oranges are planted in each mu. Each group was cultivated and managed according to the cultivation methods of the corresponding examples 1 to 3 and comparative examples 1 to 7. The average girdling rate per mu in the bud picking and flowering period, the average girdling rate per mu in the summer tip period, the average girdling rate per mu in the autumn tip period and other technical indexes are measured, and the statistics are shown in table 2.
Table 2:
Figure BDA0002733274480000102
the long term cultivation statistics from tables 1 and 2 show that:
unlike example 1, the example 2 flush fertilizer has a very low girdling rate and avoids girdling throughout the year within the range of the example 1.
The root fertilizer application mode in example 3 is different from that in example 1, a small number of plants need to be cut in a cultivation process, and the analysis shows that the water flush fertilizer and the root system restoration fertilizer can induce more new root systems to generate, and under the common water absorption effect of old roots and new root systems, the concentration of root system cell sap is diluted, the leaf surface-root system water and nutrition circulation effect is increased, and a small amount of cost of manpower and material resources is generated.
Comparative example 1 differs from example 1 in that the water flush application step S1 does not include waste honey solution, and the girdling rate in the cultivation process is higher than that in example 1. Analysis shows that the comparative example 1 affects the proliferation culture of mixed flora, and the root is difficult to replace harmful flora in soil after applying water flush fertilizer, so that humic cyclic flora is difficult to construct.
Comparative example 2 differs from example 1 in that the water flush fertilizer does not include fish meal and corn meal, and the cultivation process has a higher girdling rate than example 1. Analysis shows that comparative example 2 affects the proliferation culture of mixed flora, and the replacement of harmful flora in soil after applying water flush fertilizer to roots is difficult, and it is difficult to construct humic cyclic flora.
Comparative example 3 is different from example 1 in that the water flush fertilizer does not include soybean meal and rice bran, and the girdling rate during cultivation is higher than that of example 1. Analysis shows that comparative example 3 affects the metabolic capacity of the mixed flora to degrade cellulose into small molecule sugars and humic acid.
Comparative example 4 differs from example 1 in that the deep fermentation with a water flush does not include soybean meal and wheat bran, and the girdling rate during cultivation is higher than that of example 1. Analysis shows that comparative example 4 affects the production of a large amount of humic acid component by mixed flora, affects the nutrient components of the flush fertilizer and affects the ability to raise the concentration of plant cell sap.
Comparative example 5 differs from example 1 in that the water flush fertilizer does not include chitosan and seaweed extract, the seaweed extract contains rich algal polysaccharide, and the girdling rate during cultivation of comparative example 5 is higher than that of example 1. Analysis shows that the fermentation process of comparative example 5 does not comprise chitosan and algal polysaccharide, so that the fermentation is influenced to produce amino-oligosaccharide components, citrus root systems lack of absorption of the amino-oligosaccharide components, the generation of new root systems is induced, the absorption effect of nutrient paste is reduced, and the plant cell sap concentration increasing capacity is influenced.
Comparative example 6 is different from example 1 in that the root system restoration fertilizer does not include waste honey solution, and the girdling rate in the cultivation process is higher than that in example 1. Analysis shows that the root system restoration fertilizer of the comparative example 6 does not comprise waste honey liquid, so that the root system restoration fertilizer applied in summer-top period lacks small molecular carbon sources such as oligosaccharide, polysaccharide, oligopeptide, amino acid and the like to cultivate and reproduce humic cyclic flora planted in soil, and the concentration of plant cell sap is difficult to maintain in high concentration all year round.
The comparative example 7 is different from example 1 in the raw material and the preparation method of the water flush fertilizer, and the girdling rate in the cultivation process is higher than that in example 1. Analysis shows that in comparative example 7, a dominant bacterial group cannot be formed by using a large amount of commercial humic acid and fulvic acid for mixed fermentation with other raw materials and thalli, a humic cyclic bacterial group cannot be constructed, crude fibers in other raw materials cannot be decomposed to generate a large amount of humic acid, fulvic acid and other nutritional ingredients, and the number of free radicals of the commercial humic acid and fulvic acid is different from that of humic acid and fulvic acid obtained by multi-level raw material fermentation, the physiological activities are different, other nutritional ingredients are different, and citrus root systems absorb different effects, so that the concentration of plant cell sap is influenced.
Although embodiments of the invention have been disclosed above, they are not limited to the use listed in the specification and embodiments. It can be applied to various fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. Therefore, the invention is not to be limited to the specific details and embodiments shown and described herein, without departing from the general concepts defined in the claims and their equivalents.

Claims (7)

1. The method for culturing the citrus plant cell sap without girdling by controlling the concentration is characterized by comprising the following steps of:
the method for retaining fruiting flower buds and retaining fruiting parent branches in the next year comprises the following steps: culturing spring tips each year as fruiting branches and fruiting parent branches of the next year, and wiping off excessive flower buds on the fruiting branches in the spring tip extraction period each year to leave fruiting flower buds; after the spring tips are planted, a plurality of flower-free spring tips are reserved and are cultivated into fruiting parent branches in the next year;
the fertilizing method comprises the steps of applying root fertilizer and spraying Shi Yuhua Ling No. 2 foliar fertilizer: the root fertilizer application is as follows: applying root system repairing fertilizer in summer for 10-20 days after flowers are removed each year by applying root Shi Chong fertilizer 1-2 months each year; the spray Shi Yuhua Ling No. 2 foliar fertilizer is: spraying Shi Yuhua Ling No. 2 foliar fertilizer in the spring tip stage to promote and strengthen flowers and reduce the nutrition growth requirement of spring tips; spraying Shi Yuhua Ling No. 2 foliar fertilizer in the summer shoot period to supplement nutrition to the tree body and reduce the nutrition growth requirement of the summer shoot; spraying Shi Yuhua Ling No. 2 foliar fertilizer in autumn and winter to promote flower bud differentiation in the next year and reduce nutrition growth requirements of autumn and winter;
the pruning method comprises the following steps: trimming useless tips of the grown spring tips, xia Shao tips, autumn tips and winter tips;
wherein, the fertilizing amount of the root system restoring fertilizer is calculated according to the yield: applying 1.0-3.5 kg of root repairing agent, 1.0-1.5 kg of waste honey liquid, 1.0-2.0 kg of peanut cake, 0.8-1.0 kg of potassium sulfate, 0.1-0.2 kg of lime powder, 0.01-0.15 kg of yeast extract and 0.1-0.8 kg of dipotassium hydrogen phosphate to each 60-100 kg of plant;
fertilizer application amount of the water flush fertilizer is calculated by yield: applying 2.5-5 kg of water flush fertilizer to each 60-100 kg of plants per yield; the water flush fertilizer is prepared from the following raw materials in parts by mass:
s1, mixing 20-40 parts of waste honey liquid, 5-15 parts of bean pulp, 6-13 parts of corn meal, 5-10 parts of fish meal, 5-10 parts of seaweed extract, 1-5 parts of rice bran and 20-40 parts of water;
s2, taking the mixture obtained in the step S1, regulating the pH value to 5.5-7.5 by using 1M dipotassium hydrogen phosphate or potassium dihydrogen phosphate buffer solution, uniformly mixing, and sterilizing at the high temperature of 120 ℃ for 30-70min to prepare a water flush fertilizer culture medium;
s3, mixing 30-55 parts of bacillus subtilis, 10-20 parts of saccharomycetes and 5-8 parts of bacillus amyloliquefaciens to obtain organic matter degrading bacterial powder;
s4, the mass ratio of the organic matter degrading bacteria powder to the water flush fertilizer culture medium is 1:50-100, stirring and fermenting at 25-30deg.C for 3-5 days;
s5, adding 10-12 parts of wheat bran, 30-45 parts of bean pulp powder and 5-7 parts of chitosan into the fermentation broth obtained in the step S4, and stirring and fermenting for 2-3 days at 40-50 ℃;
s6, mixing 0.5-1 part of neutral cellulase and 0.2-0.5 part of alkaline protease to form a degradation enzyme system, wherein the mass ratio of the degradation enzyme system to the fermentation liquor in the step S5 is (1): 50-100, and fermenting and decomposing for 7-10 days to obtain the water flush fertilizer;
the root system repairing agent is prepared from the following raw materials in parts by mass: 89 parts of waste honey liquid, 8 parts of goat horn ash, 0.4 part of saccharomycetes and 0.2 part of ferment bacteria, wherein the goat horn ash is powder prepared by soaking, drying, burning and crushing waste goat horns; the Yuhualiing No. 2 foliar fertilizer comprises the following components in parts by mass: 94 parts of waste honey liquid, 3 parts of amino acid, 0.8 part of choline chloride, 0.4 part of saccharomycetes, 0.2 part of ferment bacteria, 0.8 part of urea, 0.5 part of ferrous sulfate, 0.2 part of boric acid, 0.05 part of ammonium molybdate, 6 parts of claw beam ash and 10 parts of glycerin, wherein the Yuhualing No. 2 foliar fertilizer is diluted 180-200 times when being sprayed.
2. The method for culturing citrus plant cell sap concentration control loop-free according to claim 1, wherein the retaining of the plurality of flower-free spring tips is based on retaining 1-3 small branches and 1-4 thick branches according to the size of a basal branch, and the rest is wiped off, so that nutrition is supplied to the retained flower buds and the retained flower-free spring tips in a concentrated manner.
3. The method for cultivating citrus plant cell sap concentration control girdling-free according to claim 2, wherein the method for applying the water flush fertilizer is to uniformly spread the water flush fertilizer under the tree crown, after the fertilizer is applied, the fertilizer, weeds and dry branches and fallen leaves are turned over, part of citrus root systems are cut off and uniformly mixed in soil, the rotary cultivation position is 0.1-0.8 m away from the base of the tree head, the turning-over area is 20-50% of the orchard, the depth is 25-40 cm, and then the orchard is covered with the ground cloth.
4. The method for cultivating citrus plant cell sap concentration control girdling-free according to claim 3, wherein the root system restoration fertilizer is uniformly spread under the tree crown, fertilizer, weeds and dead branches and fallen leaves are turned over after the fertilizer is applied, part of citrus root systems are cut off and uniformly mixed in soil, the rotary cultivation position is 0.1-0.8 m away from the base of the tree head, the turning-over area is 20-50% of the orchard, the depth is 25-40 cm, and then the orchard is covered with the whole field of land cloth.
5. The method for citrus plant cytofluid concentration-controlled girdling-free cultivation according to any one of claims 1 to 4, wherein said spraying Shi Yuhua number 2 foliar fertilizer in spring tip stage comprises spraying said number 2 foliar fertilizer during and before the bud picking of the flowering buds in spring tip stage, and spraying said number 2 foliar fertilizer during the first and second physiological fruit dropping stages;
the spray application of the Yuhualing No. 2 foliar fertilizer during the seeding flower bud in the spring tip period and before the flower bud is wiped out comprises the following specific steps: under the condition of extremely small or no flower quantity, spraying the Yuhualiling 2 foliar fertilizer mixed solution of Yuhualiling 2 foliar fertilizer and ethephon foliar fertilizer once when the spring tips are 1-3 cm long, wherein the concentration of ethylene Li Zaisuo in the Yuhualiling 2 foliar fertilizer mixed solution is lower than 200ppm, and spraying the Yuhualiling 2 foliar fertilizer once again 5-7 days after spraying;
the spraying of the Yuhualiing No. 2 foliar fertilizer in the first and second physiological fruit dropping periods specifically comprises the following steps: under the condition of large flower quantity, spraying the Yuhualing No. 2 foliar fertilizer once when young spring tip leaves are unfolded; under the condition of insufficient flower quantity, when spring tips grow 1-3 cm, spraying the Yuhualiing No. 2 foliar fertilizer once;
the leaf fertilizer for spraying Shi Yuhua Ling No. 2 in the summer tip period comprises the following specific components: when flowers are reduced to 80-85%, spraying the Yuhualiing No. 2 foliar fertilizer once, if the nutrition of the fruit tree is insufficient, the summer shoots are early in growth, and when the summer shoots are exposed to white, spraying the Yuhualiing No. 2 foliar fertilizer once;
the Shi Yuhua Ling No. 2 foliar fertilizer sprayed in autumn and winter is specifically: continuously spraying the Yuhualiing No. 2 foliar fertilizer twice in the late autumn and early winter, and spraying the foliar fertilizer for the second time at intervals of 7-10 days; if the temperature is high in winter, or the rainwater is too high, or the tree vigor is more vigorous, the mixed liquid of the Yuhualiling 2 foliar fertilizer added with the paclobutrazol is sprayed once, and the concentration of the paclobutrazol in the mixed liquid of the Yuhualiling 2 foliar fertilizer is 500ppm, so that the flower bud differentiation of the next year is promoted.
6. The method for culturing citrus plant cell sap concentration control loop-free according to claim 5, wherein the pruning target of the pruning method is that young shoots are not pulled out after pruning, and fruits are prevented from direct irradiation of strong light and are taken care of bore branches and absorb sunlight; the time selection of the pruning method is based on that the trees planted in the relatively weak or dry land with more results are pruned early, the trees planted in the paddy field with less results are pruned late; the pruning method has the following pruning standards for branch tips: the main branch tips of the result are reserved, the rest of pest branches, weak branches, residual branches after fruiting and excessive branches are cut off, the tree body is ventilated and transparent, and the result is that the Xia Shao, autumn tips and winter tips which are extracted and generated on the main branch are properly cut off or cut off according to the size and height of the crown.
7. The method for loop-free cultivation of citrus plant cell sap concentration control according to claim 6, wherein the pruning method further comprises quantifying shoot retention, the quantified shoot retention criteria being: setting a tree with the yield of 30-40 kg for the fruit tree, and keeping 30-50 branches of the fruiting parent branches; setting a tree with the yield of 40-60 kg for the fruit tree, and keeping 50-80 branches of parent branches as a result; the yield of the fruit tree is set to be 60-110 kg, and the number of the reserved parent branches is 70-100.
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