CN102805401A - Chinese caterpillar fungus health-care drink prepared by liquid deep submerged fermentation and preparation method thereof - Google Patents
Chinese caterpillar fungus health-care drink prepared by liquid deep submerged fermentation and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a Chinese caterpillar fungus health-care drink prepared by liquid deep submerged fermentation and a preparation method thereof. The preparation method comprises the following steps of: (A), inoculating Chinese caterpillar fungus strains into a culture solution, and performing shake culture on a shaker to obtain strain liquid; (B), inoculating the strain liquid obtained in the step (A) into a liquid culture medium containing barley malt and soybeans, and performing aerobic culture to obtain fermented Chinese caterpillar fungus mycelia and Chinese caterpillar fungus bacterial fermentation liquor; (C), crushing cell tissues of the Chinese caterpillar fungus mycelia obtained in the step (B), and extracting by using water to obtain a Chinese caterpillar fungus mycelium extracting solution; and (D), mixing the Chinese caterpillar fungus bacterial fermentation liquor obtained in the step (B) and the Chinese caterpillar fungus mycelium extracting solution obtained in the step (C) to obtain a mixed solution, diluting the mixed solution by adding water until the concentration of the mixed solution is a half to one fifth of the original concentration to obtain the Chinese caterpillar fungus health-care drink. Due to the adoption of a liquid deep submerged fermentation technology, Chinese caterpillar fungus strains can be massively propagated industrially in the liquid culture medium; and the preparation method is simple in process and low in cost.
Description
Technical field
The present invention relates to a kind of beverage and preparation method thereof, particularly relate to a kind of aweto health-care drink that passes through the liquid deep layer fermenting preparation and preparation method thereof.
Background technology
Cordyceps sinensis [formal name used at school Cordyceps (Ophiocordyceps) sinensis; Hirsutella sinensis] belong to Ascomycotina, Clavicipitaceae, Cordyceps, be the famous and precious Chinese herbal medicine class fungi of China's tradition, have the title of " king of medicine ", with genseng, pilose antler and be called Chinese medicine three big tonics.Since ancient times, Cordyceps sinensis as excellent tonic product in China by extensive approval and use.Modern science and technology shows; Composition such as contained cordycepic acid and cordycepin in the Cordyceps sinensis; Have expansion tracheae, calmness, antibacterium, hypotensive effect, treatment pulmonary tuberculosis, cough, weak, anaemia is weak, spontaneous sweating, aspects such as void, the haematemesis of Lip river blood, chronic nephritis all have good effect for a long time after being ill.Research shows: the aweto mycelium that fermentation produces is identical with the natural cordyceps effect, and both all are rich in function factors such as Chinese caterpillar fungus polysaccharide, amino acid, sweet mellow wine and cordycepin.
At present, correlative study both domestic and external mainly concentrates on extraction and pharmacology, the drug effect of Cordyceps sinensis function factor.But the artificial cultivation field at cordyceps sporophore but receives a lot of technical limitations, carries out suitability for industrialized production and civil nature use widely seldom on a large scale, and simultaneously, this technical field also is difficult to commercialization and uses.Before 10 years, the year recoverable amount of occurring in nature cordyceps sporophore has 10,000 tons approximately, 5 kilotons but present recoverable amount is but only had an appointment; And; As stated, cordyceps sporophore is difficult to a large amount of manual work production again owing to technical reason; Therefore, people have produced significant contradiction to the shortage of craving for the cordyceps sporophore recoverable amount of Chinese caterpillar fungus health-care effect and therapeutic efficiency.
Summary of the invention
The objective of the invention is to; Overcome the defective of above-mentioned prior art and a kind of suitability for industrialized production of can carrying out on a large scale is provided, and can produce the method that liquid deep layer fermenting prepares aweto health-care drink of passing through of the higher cordyceps sporophore of effectiveness with lower cost.
For realizing above-mentioned purpose, the present invention takes following design:
A kind of method for preparing aweto health-care drink through liquid deep layer fermenting of the present invention; Preparation method of the present invention comprises the steps: A, aweto is inserted in the nutrient solution; And under 18～28 ℃ temperature, on shaking table, shake cultivation 72～96 hours with 200～300 times/minute concussion frequency, obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the aweto mycelium and the cordyceps militeris fungus fermentation broth of fermentation; C, the aweto mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the aweto mycelium extract; D, the aweto mycelium extract that obtains among cordyceps militeris fungus fermentation broth that obtains among the step B and the step C mixed each other after, with 2～5 times of mixed liquor dilutions, thereby obtain aweto health-care drink.
Preferably, water or mycelium extract dilute 2～5 times with said mixed liquor in said step D.
Preferably, in said dilution, also add the beverage auxiliary material, said beverage auxiliary material comprises: one or more in sucrose, citric acid, herbal glucoside extract and the honey.
Preferably, in said step B, the index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
Preferably, in said step D, the mutual mixed natural pH value of cordyceps militeris fungus fermentation broth and aweto mycelium extract is 6～7, and the refractive power pol is 2～6%.
Preferably, in the cordyceps militeris fungus fermentation broth that obtains among the said step B, contain 0.1～5mg/100g adenosine and 10～100mg/100g Cordyceps sinensis polysaccharide.
Preferably, in the aweto mycelium extract that obtains among the said step C, contain 0.5～10mg/100g adenosine and 10～200mg/100g Cordyceps sinensis polysaccharide.
Another object of the present invention is to provide a kind of aweto health-care drink through the liquid deep layer fermenting preparation; Said aweto health-care drink is to be prepared from following method: A, aweto is inserted in the nutrient solution; And under 18～28 ℃ temperature, on shaking table, shake cultivation 72～96 hours with 200～300 times/minute concussion frequency, obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the aweto mycelium and the cordyceps militeris fungus fermentation broth of fermentation; C, the aweto mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the aweto mycelium extract; D, the aweto mycelium extract that obtains among cordyceps militeris fungus fermentation broth that obtains among the step B and the step C mixed each other after, with 2～5 times of mixed liquor dilutions, thereby obtain aweto health-care drink.
Preferably, water or mycelium extract dilute 2～5 times with said mixed liquor in said step D.
Preferably, in said dilution, also add the beverage auxiliary material, said beverage auxiliary material comprises: one or more in sucrose, citric acid, herbal glucoside extract and the honey.
Advantage of the present invention is: the present invention adopts that the aweto in bacterial classification original producton location (Qinghai-Tibet Platean) separates, purifying and cultivation; Adopt industrialization liquid deep layer fermenting technology, make aweto strain to carry out a large amount of breedings in the liquid medium within, and; All contain in the metabolite of gained and the mycelium and the identical active ingredient of former aweto effectiveness; In addition, the present invention also makes the fructification of aweto to produce and to cultivate in industrialized condition, and technology is simple, cost is controlled; Thereby can reduce production costs significantly, make ordinary consumer also can use famous and precious health drink.
The specific embodiment
Embodiment 1:
Following prepare the preferred embodiment of the method for aweto health-care drink for the present invention is a kind of through liquid deep layer fermenting, preparation method of the present invention comprises following key step:
A, at first; Aweto is inserted in the nutrient solution; Nutrient solution of the present invention can be the various nutrient solutions that are suitable for the aweto Liquid Culture of the prior art, and under 18 ℃ temperature, on shaking table, shakes cultivation 72 hours with 300 times/minute concussion frequency, obtains strain liquid; B, then; The strain liquid access that obtains in the steps A is contained in the fluid nutrient medium of weight portion 8% fructus hordei germinatus, weight portion 6% soybean, and inoculum concentration is 5%, and ventilation was cultivated 48 hours under 27 ℃ temperature; Through biofermentation, obtain the aweto mycelium and the cordyceps militeris fungus fermentation broth of fermentation; C, again the aweto mycelium that obtains among the step B is carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.8:5, extraction temperature is 95 ℃, extraction time is 2 hours, thereby obtains the aweto mycelium extract; Further, Cordyceps fermented liquid and wet thallus that the present invention mainly utilizes industrialization liquid deep layer fermenting technology to obtain prepare and produce aweto health-care drink; D, last after the aweto mycelium extract that obtains among cordyceps militeris fungus fermentation broth that obtains among the step B and the step C mixed each other, with 2 times of mixed liquor dilutions, thereby obtains aweto health-care drink.Therefore,, popular straight drink product be can produce, health products and beverage comprised through preparation method of the present invention.And preparation method of the present invention can directly apply to suitability for industrialized production, and cost is low, and the products obtained therefrom trophic function is high, helps consumer and public daily health carings.
More specifically, preparation method of the present invention can directly carry out industrial and large-scale and produces continuously.After the batch process, can produce the cordyceps militeris fungus fermentation broth more than 10 tons every day, and produce 20 tons～final products more than 50 tons every day through preparation method of the present invention.
On the other hand, in preparation method's of the present invention step D, water or mycelium extract are with 2～5 times of said mixed liquor dilutions, and preferably, in the present embodiment, water is with 2 times of said mixed liquor dilutions; And, further, in said dilution, also adding the beverage auxiliary material, said beverage auxiliary material comprises: one or more in sucrose, citric acid, herbal glucoside extract and the honey.These auxiliary materials can improve the mouthfeel of the prepared beverage of the inventive method, make the consumer when drinking health drink of the present invention, can reduce or remove the bitter of Chinese medicine, and more be the tasty and refreshing of daily beverage.And through a certain amount of aweto health-care drink that the inventive method obtains, the content of its functional components and cordyceps sporophore are close or be higher than cordyceps sporophore, its effectiveness and directly to take Cordyceps sinensis suitable.
More preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) are preferably and reach relevant parameters separately simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed natural pH value of cordyceps militeris fungus fermentation broth and aweto mycelium extract is 6～7, and the refractive power pol is 2～6%.
On the one hand, in the cordyceps militeris fungus fermentation broth that obtains among the step B according to the invention, contain 0.1～5mg/100g adenosine and 10～100mg/100g Cordyceps sinensis polysaccharide.Therefore, in step B of the present invention in the resulting cordyceps militeris fungus fermentation broth, the data area of this two kinds of functional components (adenosine and Cordyceps sinensis polysaccharide) content meets modern pharmacology and researchs and analyses among the result numerical value identification to natural cordyceps content.
On the other hand, in the aweto mycelium extract that obtains among the step C according to the invention, contain 0.5～10mg/100g adenosine and 10～200mg/100g Cordyceps sinensis polysaccharide.Therefore; In step C of the present invention in the resulting aweto mycelium extract; The data area of this two kinds of functional components (adenosine and Cordyceps sinensis polysaccharide) content; Far exceed modern pharmacology and research and analyse numerical value scope of determination, and the functional content of the inventive method products therefrom and quality are all highly stable the natural cordyceps component content.Preparation method's of the present invention industrialization control ability is strong, and its cost is far below natural material.
Embodiment 2:
Preparation method's of the present invention another kind of preferred embodiment will be provided as follows.The preparation method of present embodiment comprises: A, at first; Select food, medicinal bacterium Cordyceps sinensis (Ophiocordyceps sinensis) for use; Aweto is inserted in the nutrient solution; And under 28 ℃ temperature, on shaking table, shake cultivation 96 hours with 200 times/minute concussion frequency, obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 12% fructus hordei germinatus, weight portion 3% soybean; Inoculum concentration is 10%; And ventilation was cultivated 72 hours under 23 ℃ temperature, through biofermentation, obtained the aweto mycelium and the cordyceps militeris fungus fermentation broth of fermentation; C, the aweto mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 1:2, extraction temperature is 90 ℃, extraction time is 3 hours, thereby obtains the aweto mycelium extract; D, the aweto mycelium extract that obtains among cordyceps militeris fungus fermentation broth that obtains among the step B and the step C mixed each other after, with 5 times of mixed liquor dilutions, thereby obtain aweto health-care drink.
Embodiment 3:
Another preferred embodiment of preparation method of the present invention will be provided as follows.The preparation method of present embodiment comprises the steps: A, aweto is inserted in the nutrient solution, and under 22 ℃ temperature, on shaking table, shakes cultivation 80 hours with 260 times/minute concussion frequency, obtains strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 10% fructus hordei germinatus, weight portion 5% soybean; Inoculum concentration is 7%; And ventilation was cultivated 60 hours under 25 ℃ temperature, through biofermentation, obtained the aweto mycelium and the cordyceps militeris fungus fermentation broth of fermentation; C, the aweto mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, lixiviate is than being 0.9:4, extraction temperature is 93 ℃, extraction time is 2.5 hours, thereby obtains the aweto mycelium extract; D, the aweto mycelium extract that obtains among cordyceps militeris fungus fermentation broth that obtains among the step B and the step C mixed each other after, with 4 times of mixed liquor dilutions, thereby obtain aweto health-care drink.
Embodiment 4:
Present embodiment provides a kind of aweto health-care drink through the liquid deep layer fermenting preparation; Said aweto health-care drink is to be prepared from following method: A, will select for use food, medicinal aweto to insert in the nutrient solution; And under 18～28 ℃ temperature (present embodiment is preferably 20 ℃), on shaking table, shake 72～96 hours (present embodiment is preferably 82 hours) of cultivation with the concussion frequency of 200～300 times/minute (present embodiment is preferably 260 times/minute), obtain strain liquid; B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% (present embodiment is preferably 11%) fructus hordei germinatus, weight portion 3～6% (present embodiment is preferably 5%) soybean; Inoculum concentration is 5～10% (present embodiment is preferably 8%); And ventilation is cultivated 48～72 hours (present embodiment is preferably 56 hours under the temperature of 23～27 ℃ (present embodiment is preferably 26 ℃); Through biofermentation, obtain the aweto mycelium and the cordyceps militeris fungus fermentation broth of fermentation; C, the aweto mycelium that obtains among the step B carried out the cell tissue fragmentation after; Water extracts; The lixiviate ratio is 0.8～1:2～5 (present embodiment is preferably 1:3); Extraction temperature is 90～95 ℃ (present embodiment is preferably 92 ℃), and extraction time is 2.5 hours, thereby obtains the aweto mycelium extract; D, the aweto mycelium extract that obtains among cordyceps militeris fungus fermentation broth that obtains among the step B and the step C mixed each other after, with 2～5 times of mixed liquor dilutions, thereby obtain aweto health-care drink.
Wherein, water or mycelium extract preferably, in said dilution, also add the beverage auxiliary material with 2～5 times of said mixed liquor dilutions in step D, and said beverage auxiliary material comprises: one or more in sucrose, citric acid, herbal glucoside extract and the honey.These auxiliary materials can improve the mouthfeel of beverage according to the invention, make the consumer when drinking health drink of the present invention, can reduce or remove the bitter of Chinese medicine, and more be the tasty and refreshing of daily beverage.And, aweto health-care drink of the present invention, the content of its functional components and cordyceps sporophore are close or be higher than cordyceps sporophore, and its effectiveness is with directly to take Cordyceps sinensis suitable.
Preferably; In the described step B of present embodiment; The index that fermentation stops is that the pH value reaches 4.0～5.5, and the refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85% simultaneously; Above-mentioned three kinds of indexs (pH value, refractive power pol, mycelia yield) reach corresponding separately index simultaneously, can stop fermentation.
In addition, in the described step D of present embodiment, the mutual mixed pH value of cordyceps militeris fungus fermentation broth and aweto mycelium extract is 6～7, and the refractive power pol is 2～6%.
Preferably, in the cordyceps militeris fungus fermentation broth that obtains among the said step B, contain 0.1～5mg/100g adenosine and 10～100mg/100g Cordyceps sinensis polysaccharide.
Preferably, in the aweto mycelium extract that obtains among the said step C, contain 0.5～10mg/100g adenosine and 10～200mg/100g Cordyceps sinensis polysaccharide.
Other embodiment of a kind of aweto health-care drink through liquid deep layer fermenting preparation of the present invention are identical or close with the foregoing description 1,2 and 3, repeat no more at this.To those skilled in the art, be not difficult from the foregoing description 1,2 and 3, to obtain relevant teachings, and in order to preparation or production aweto health-care drink.
Obviously, those of ordinary skill in the art can use a kind of aweto health-care drink that passes through the liquid deep layer fermenting preparation of the present invention and preparation method thereof, forms various types of health drinks and preparation method thereof.
The foregoing description only supplies to explain the present invention's usefulness; And be not to be limitation of the present invention; The those of ordinary skill in relevant technologies field without departing from the present invention, can also be made various variations and modification; Therefore all technical schemes that are equal to also should belong to category of the present invention, and scope of patent protection of the present invention should be limited each claim.
Claims (10)
1. prepare the method for aweto health-care drink through liquid deep layer fermenting, it is characterized in that: said preparation method comprises the steps:
A, aweto is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated 72～96 hours, obtain strain liquid with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the aweto mycelium and the cordyceps militeris fungus fermentation broth of fermentation;
C, the aweto mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the aweto mycelium extract;
D, the aweto mycelium extract that obtains among cordyceps militeris fungus fermentation broth that obtains among the step B and the step C mixed each other after, with 2～5 times of mixed liquor dilutions, thereby obtain aweto health-care drink.
2. according to claim 1ly prepare the method for aweto health-care drink through liquid deep layer fermenting, it is characterized in that: water or mycelium extract are with 2～5 times of said mixed liquor dilutions in said step D.
3. the method for preparing aweto health-care drink through liquid deep layer fermenting according to claim 2; It is characterized in that: in said dilution; Also add the beverage auxiliary material, said beverage auxiliary material comprises: one or more in sucrose, citric acid, herbal glucoside extract and the honey.
4. according to claim 2 or the 3 described methods that prepare aweto health-care drink through liquid deep layer fermenting; It is characterized in that: in said step B; The index that fermentation stops is that the pH value reaches 4.0～5.5; The refractive power pol reaches 2.8～3.8%, and the mycelia yield dry weight reaches 0.65～0.85%.
5. the method for preparing aweto health-care drink through liquid deep layer fermenting according to claim 4; It is characterized in that: in said step D; The mutual mixed pH value of cordyceps militeris fungus fermentation broth and aweto mycelium extract is 6～7, and the refractive power pol is 2～6%.
6. according to claim 1 or the 4 described methods that prepare aweto health-care drink through liquid deep layer fermenting; It is characterized in that: in the cordyceps militeris fungus fermentation broth that obtains among the said step B, contain 0.1～5mg/100g adenosine and 10～100mg/100g Cordyceps sinensis polysaccharide.
7. according to claim 1 or the 4 described methods that prepare aweto health-care drink through liquid deep layer fermenting; It is characterized in that: in the aweto mycelium extract that obtains among the said step C, contain 0.5～10mg/100g adenosine and 10～200mg/100g Cordyceps sinensis polysaccharide.
8. one kind is passed through the aweto health-care drink that liquid deep layer fermenting prepares, and it is characterized in that: said aweto health-care drink is to be prepared from following method:
A, aweto is inserted in the nutrient solution, and under 18～28 ℃ temperature, on shaking table, shake and cultivated 72～96 hours, obtain strain liquid with 200～300 times/minute concussion frequency;
B, the strain liquid that obtains in the steps A inserted contain in the fluid nutrient medium of weight portion 8～12% fructus hordei germinatus, weight portion 3～6% soybean; Inoculum concentration is 5～10%; And ventilation was cultivated 48～72 hours under 23～27 ℃ temperature; Through biofermentation, obtain the aweto mycelium and the cordyceps militeris fungus fermentation broth of fermentation;
C, the aweto mycelium that obtains among the step B carried out the cell tissue fragmentation after, water extracts, the lixiviate ratio is 0.8～1:2～5, extraction temperature is 90～95 ℃, extraction time is 2～3 hours, thereby obtains the aweto mycelium extract;
D, the aweto mycelium extract that obtains among cordyceps militeris fungus fermentation broth that obtains among the step B and the step C mixed each other after, with 2～5 times of mixed liquor dilutions, thereby obtain aweto health-care drink.
9. the aweto health-care drink through the liquid deep layer fermenting preparation according to claim 8 is characterized in that: water or mycelium extract are with 2～5 times of said mixed liquor dilutions in said step D.
10. the aweto health-care drink through the liquid deep layer fermenting preparation according to claim 8; It is characterized in that: in said dilution; Also add the beverage auxiliary material, said beverage auxiliary material comprises: one or more in sucrose, citric acid, herbal glucoside extract and the honey.
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| CN104054504A (en) * | 2013-03-20 | 2014-09-24 | 陆九妹 | Cultivation method of bee pupa cordyceps militaris |
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| CN104522812A (en) * | 2014-12-30 | 2015-04-22 | 华南师范大学 | Cordyceps active lactic acid bacteria beverage and production method thereof |
| CN106213479A (en) * | 2016-07-30 | 2016-12-14 | 成都薇诺娜生物科技有限公司 | A kind of health product containing Cordyceps and preparation method |
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| CN104522812B (en) * | 2014-12-30 | 2016-04-20 | 华南师范大学 | A kind of Cordyceps sinensis viable bacteria beverage and production method thereof |
| CN106213479A (en) * | 2016-07-30 | 2016-12-14 | 成都薇诺娜生物科技有限公司 | A kind of health product containing Cordyceps and preparation method |
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