CN1164571A

CN1164571A - Cordyceps sinensis health-care nutrient liquor produced by submerged fermentation method

Info

Publication number: CN1164571A
Application number: CN96119701A
Authority: CN
Inventors: 张世伟
Original assignee: Individual
Current assignee: Individual
Priority date: 1996-12-13
Filing date: 1996-12-13
Publication date: 1997-11-12
Anticipated expiration: 2016-12-13
Also published as: CN1055967C

Abstract

The present invention relates to a cordyceps health-care nutrient liquor produced by submerged fermentation process. Its technological process includes: slant culture of cordyceps sinensis mycelium; twice strain shaking culture; first-stage and second-stage strain tank culture; submerged fermentation culture in fermentation tank; filtering, extracting and respectively collecting filtrate and mycelium; making them into cordyceps health-care nutrient liquor; sterilizing and packaging so as to obtain the invented finished product. It is a tonic product containing 18 amino acids, including 8 amino acids necessary for human body.

Description

The cordyceps sinensis health-care nutrient liquor that tank fermentation method is produced

The invention discloses the cordyceps sinensis health-care nutrient liquor that a kind of tank fermentation method is produced, it belongs to the medicine industry technical field, also belongs to the health food technology field, also belongs to technical field of bioengineering.The preserving number of bacterial classification of the present invention at China typical culture collection center is S-W229 CCTCC M94052.

Cordyceps sinensis is a kind of medicinal fungi, belongs to Ascomycetes, Clavicipitaceae, and Cordyceps, Cordyceps [Cordycepssinensis (Berk) Sacc], it is a kind of rare traditional Chinese medicine, can compare favourably with genseng, pilose antler.Be one of three big tonics.The literature record the earliest that Cordyceps sinensis has a medicinal efficacy is " new compilation of materia medica " that (clear Qianrong 22 years) Wu Yi Lip river in 1757 is write, and it spells out the effect that Cordyceps sinensis has " protect the lung kidney-nourishing, hemostasis and phlegm consumptive disease is coughed ".In fact, people in practical experience of life with Cordyceps sinensis as medicinal medicinal material, much more Zao than literature record.The compatriot of Tibetan is used as medicine with it already, is referred to as " Ya Zhagongbu ", and literal translation is exactly a Cordyceps sinensis.

Cordyceps sinensis is tonic, and this is known already, truly has about Cordyceps sinensis in supplementary Amplifications of the Compendium of Materia Medica, " study is wantonly examined " and controls deficient record.Cordyceps sinensis can studies have shown that it contains 18 seed amino acids for many years as excellent tonic product through China, comprising 8 seed amino acids of needed by human, also contain the necessary vitamins B of human body hematopoiesis ₁₂These compositions can be cured the disease, and also can nourish the body.But effective ingredient that Cordyceps sinensis really can be cured the disease is compositions such as cordycepic acid contained in the Cordyceps sinensis that modern science and technology have been identified and cordycepin, and these compositions have expansion tracheae, calmness, anti-various bacteria, hypotensive effect.According to relevant medical data among the people: Cordyceps sinensis is used for the treatment of pulmonary tuberculosis, old man's chilly, cough more, weak, diseases such as anaemia is weak, seminal emission impotence, spontaneous sweating, soreness of waist and knee joint, neurasthenia, empty for a long time, spitting of blood haematemesis after being ill, chronic nephritis all obtain good effect.

Nineteen sixty generation just once the someone report in the mycelium nutrient solution of pupa worm [Cordyceps militaris (L.) Link] that a kind of meta-bolites is arranged---Cordyceps sinensis element (Cordycepin) has restraining effect to mouse ehrlich carcinoma (EC) and KB cell, there was the extract of human natural cordyceps that the mouse ehrlich carcinoma has been carried out anticancer test in 1979, finds that this extract has the obvious suppression effect to EC.In recent years, the artificial culture mycelium extract of many human Cordyceps sinensis is done antitumous effect research, finds that it has certain antitumor action.

Because Cordyceps sinensis has multiple medical treatment and nourishing function, in recent years, people are increasing to its demand, and have been in great demand all over the world.Be distributed in view of wild cordyceps on 3500～4000 meters above sea level hillside of high mountain steppe, growth cycle is long, and the Cordyceps sinensis resource is subjected to very big destruction in recent years in addition, causes wild cordyceps resource critical shortage.According to nineteen forty-one Japan's holt statistics, Cordyceps sinensis is complete to belong to totally 137 kinds, in state-owned 26 kinds, parasitize insect more than 90% in full the genus.And the ecotope harshness that this lepidopterous insects requires is difficult to carry out artificial culture, and therefore, China scientific worker just begins to carry out the research work that artificial fermentation is cultivated Cordyceps sinensis from generation nineteen sixty.The employing that has bacterial classification inoculation to the bat moth larvae; The employing animal protein protein that has is made the component of substratum, and industrial scale is still little, and the mouthfeel of oral liquid is undesirable.

The objective of the invention is: for overcoming the harshness that Cordyceps sinensis parasitizes lepidopterous insects requirement ecotope, the present invention intends proposing a kind of artificial culture method, feed intake, inoculation is after the product of submerged fermentation, its fermented liquid promptly can be made into the Chinese caterpillar fungus health-care oral liquid, its mycelium then can directly be processed into efficient nourishing finished product, and no waste excretes in biotechnology production, and is free from environmental pollution.In substratum, intend removing animal protein, replace animal protein with agricultural byproducts.In addition, the Chinese caterpillar fungus health-care oral liquid of making should be that smell delicate fragrance, mouthfeel are good, the damp opal oral liquid of bag.

For realizing that the technical measures that the object of the invention is taked are: fermentation method is cultivated the Cordyceps mycelium technical process and is comprised cultivation, extraction, sterilization, encapsulation.(1) cultivates: the slant culture that is mixed with the mycelium bacterium culture medium with vegetable protein protein, soybean sprout juice, glucose, starch, aminoacids complex, shake a bottle bacterial classification seed culture through twice bacterial classification, again through one-level, second class inoculum seed culture, doing submerged fermentation at fermentor tank then cultivates, the submerged fermentation back that finishes is carried out and to be put jar, and liquid and mycelium can mix up and be health-care nutrient liquor.(2) extract: adopt the high speed frozen centrifugation, centrifugation rate 14000rps/M, centrifugal 20 minutes, fermented liquid is filtered, collect Cordyceps mycelium and filtrate respectively.(3) formulated: 3.1 cordyceps sinensis mycelium powder agent: to Cordyceps mycelium decompression cryodrying, pulverize then, obtain pulvis.3.2 mycelium extracts thing: with comprising that the alcoholic acid solvent extracts active substance adenosine, uridylic etc.3.3 the compositing formula of substratum: its prescription is: the vegetable protein protein accounts for 〉=and 2 to 7%; Soybean sprout juice accounts for 〉=and 0.5 to 1.2%; Starch accounts for 〉=and 4 to 6%; Glucose accounts for 〉=and 4 to 6%; Aminoacids complex accounts for 〉=and 0.1 to 0.3%; All the other are water.Oral health nutrient liquid formulation pack into preparation and in after sterilization i.e. sealing form product.

Compared with the prior art, the technique effect that the present invention has reached: the sense index of cordyceps sinensis health-care nutrient liquor of the present invention: be milky white liquid, contain oyster white pearl mycelium pellet, be little sweet tart flavour, free from extraneous odour, no stink, inclusion-free.Physical and chemical index: the supernatant liquor that centrifugation goes out behind the ferment tank, it contains adenosine 10.09 μ g/ml, uridylic 1.5 μ g/ml.Mycelium contains adenosine 7.3 μ g/g, uridylic 3.5 μ g/g.After centrifugal, supernatant liquor contains glycosaminoglycan 0.47mg/ml, and mycelium contains glycosaminoglycan 29mg/g.Microbiological indicator: total plate count (individual/ml)≤100; Coliform (individual/100ml)＜3; Pathogenic bacterium do not detect.Quality index: reducing sugar 〉=10mg/ml; Amino acid＞100mg/100ml.Physical and chemical inspection, Micro biological Tests are carried out by national GB standard.In the quality standard, reducing sugar is by national GB standard test; Aminoacids content is the data by Hitachi's 835-50 type amino acid series analysis-e/or determinings.

Cordyceps sinensis health-care nutrient liquor of the present invention is rich in polysaccharide, amino acid, also contains the required vitamins B of human body hematopoiesis ₁₂After measured, product of the present invention contains 18 seed amino acids of constitutive protein matter composition.Wherein, human body is can not 8 kinds of essential amino acid content of self synthetic higher, the arginine and the Histidine of particularly relevant being called as at ordinary times " half is essential " with children's intelligence and anthropometic, and its content is also very high.Glycosaminoglycan is to have proved for test as the material of enhancing immunity ability.Just have glycosaminoglycan in the polysaccharide that nutritive medium of the present invention contains, it is the slow tonic of a kind of ideal warm nature.

Cordyceps sinensis health-care nutrient liquor of the present invention belongs to nontoxic level through " food safety toxicology test " acute toxicity test; Three mutagenicity tests are all negative, show that this is tried thing and does not have obvious genetoxic.Through " bone marrow micronucleus test ", consistent negative with the control group result, compare with positive controls, difference is (P＜0.01) extremely significantly.Through " salmonella/microsomes test mutagenicity test ", evaluation method is for beaming back parameter certainly above positive more than 1 times, and test result is originally tried, and the thing bacterium colony returns parameter and negative control group more surpasses 1 times, and the result is negative." sperm malformation test " result and negative control group compare, as a result basically identical.

Below in conjunction with embodiment, technical scheme of the present invention is described further:

Strain inclined plane substratum: be made into slant medium with vegetable-protein protein, glucose, starch.To carry out slant strains with the same winter worm summer herb thallus that is preserved in Chinese typical culture collection center preservation cultivates, twice this mycelium cultivated in 500ml and 5000ml triangular flask then, again in 1001,5001 one-levels, secondary seed jar, twice this mycelium seed culture, follow this mycelium of fermentation culture in 5 tons of fermentor tanks, through 25-30 ℃, pH value spontaneous fermentation 4～6 days, after fermentation finishes, put jar, filter, collect filtered liquid and mycelium respectively, make health-care nutrient liquor, form product through sterilization, encapsulation.

Claims

1. preserving number is the Cordyceps strain of the tank fermentation method of S-W229 CCTCC M94052, and it is characterized in that: it belongs to the capsule Gammaproteobacteria, Clavicipitaceae, and Cordyceps, Cordyceps fungus, its Latin is called Cordyceps Sinensis (Berk) Sacc.

2. the cordyceps sinensis health-care nutrient liquor produced of a tank fermentation method, it is characterized in that: technical process of the present invention comprises cultivation, extraction, sterilization, encapsulation, earlier with slant medium to the mycelium slant culture, shake a bottle bacterial classification seed culture through twice bacterial classification,, in fermentor tank, do submerged fermentation then and cultivate again through one-level, second class inoculum jar seed culture, after fermentation finishes, filter, extract, collect Cordyceps mycelium and filtrate respectively, form product through sterilization, encapsulation.

3. by the described cordyceps sinensis health-care nutrient liquor of claim 2, it is characterized in that: the temperature that mycelium is cultivated is 〉=25 to 30 ℃; Incubation time is 〉=4 to 6 days.

4. by claim 2 or 3 described cordyceps sinensis health-care nutrient liquors, it is characterized in that: the prescription of substratum of the present invention for the vegetable protein protein account for 〉=2 to 7%; Soybean sprout juice accounts for 〉=and 0.5 to 1.2%; Starch accounts for 〉=and 4 to 6%; Glucose accounts for 〉=and 4 to 6%; Aminoacids complex accounts for 〉=and 0.1 to 0.3%; All the other are water.

5. by the described cordyceps sinensis health-care nutrient liquor of claim 2, it is characterized in that:

A. the filtering centrifugal rotational speed of frozen centrifugation is the 14000rps/ branch, centrifugation time 20 minutes;

B. isolated Cordyceps mycelium, the decompression cryodrying gets the cordyceps sinensis mycelium powder agent after the pulverizing;

C. mycelium extracts thing, with comprising that the alcoholic acid solvent extracts adenosine and uridylic active substance;

D. put liquid and the mixed health-care nutrient liquor of making of mycelium that jar goes out after fermentation finishes.