CN102813193A - Chinese caterpillar fungus cyanine oral liquid and preparation method thereof - Google Patents
Chinese caterpillar fungus cyanine oral liquid and preparation method thereof Download PDFInfo
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Abstract
The invention discloses Chinese caterpillar fungus cyanine oral liquid which comprises the following components expressed in weight parts: 10 to 20 parts of an extract solution of Chinese caterpillar fungus mycelia, 10 to 20 parts of an extract solution of anthocyanidin and 2 to 5 parts of a concentrated solution of cordyceps militaris. The invention also discloses a preparation method for the Chinese caterpillar fungus cyanine oral liquid. Based on the principle that the above mentioned medicines are compatible and mutually synergistic, the method improves the disadvantage of insufficient efficacy when Chinese caterpillar fungus or anthocyanidin is individually used. The prepared Chinese caterpillar fungus cyanine oral liquid has good taste and the effects of lung moistening, phlegm reducing, blood sugar adjusting and the like and can enhance immunity, refresh one's spirit, treat insomnia and allow people to glow when taken for a long time.
Description
Technical field
The invention belongs to the technical field of health food exploitation and food engineering, be specifically related to a kind of Chinese caterpillar fungus cyanine oral liquid and preparation method thereof.
Background technology
Chinese caterpillar fungus is a kind of Chinese medicine, has qi-restoratives newspaper, beneficial vital essence, protects lung, kidney-nourishing, hemostasis and phlegm, the effect of strengthening by means of tonics.In recent years discovered calmness, anti-anoxic, anti-inflammatory, step-down, improved myocardial ischemia, increased immune function of human body and certain anticancer effect is arranged.The mycelia of Cordyceps militaris is a kind of sac fungus, and its phorozoon is that pupa is drawn up mould.Can form ascospore (breeding unit) after its thalline maturation, propagate with the wind after spore distributes, spore drops on the suitable polypide, just begins to sprout the formation mycelium.The mycelium one side is constantly grown, and one side begins in polypide, to spread, and the pupa worm is decomposed the tissue in the pupal cell by fungal infection,, at last pupal cell inside is decomposed fully as its matter and energy that grows source with the nutrition in the pupal cell.After general mycelia when Cordyceps militaris finished the various tissues in the pupal cell and organ decomposition, hyphostoma development had also got into a new stage.Mycelium begins to transfer to reproductive growth by nourishing and growing, stretch out from the head of pupal cell ghost, chest, caudad etc. kink back at last, forms the bar-shaped stroma (fructification) that is that the top of crocus or Chinese red slightly expands.
Silkworm chrysalis Cordyceps sinensis is that wild CORDYCEPS bacterium is inoculated in the Chinese caterpillar fungus complex that silkworm pupa forms; To human non-toxic; Contain pharmaceutical components such as abundant sweet mellow wine, ergosterol, adenosine, Cordyceps sinensis polysaccharide, drug effect and wild CORDYCEPS grass basically identical have the body immunity of raising, reducing blood lipid and the effect of male sex hormone appearance; But the substitute folk prescription that can be used as wild CORDYCEPS summer grass is taken; Also can be used as the use of medical material compatibility or process health care, medicinal product, the key technical indexes is basic identical with Cordyceps sinensis.
Anthocyanidin (anthocyanins) is that one type of occurring in nature extensively is present in the water-soluble natural pigment in the plant, belongs to flavone compound, is a kind of main present-color material in plant flowers and the fruit.Modern medicine experiment proof anthocyanidin has enough enhancing blood vessel elasticities to human body, improves the circulatory system and the smoothness of promoting skin, inflammation-inhibiting and allergy; Improve the multiple medical care effects such as pliability in joint, anthocyanidin has strong anti-oxidant, removes free radical; Improve VAR, reduce the effect of capillary permeability, its oxidation resistance be 50 times of vitamin E with ascorbic 20 times of V; The disease that causes by free radical be can also prevent and treat, heart disease, arthritis etc. comprised; Take for a long time and can effectively play the prevention cardiovascular and cerebrovascular disease, antitumor radiation-resistant effect.The primary raw material that is used to extract anthocyanidin at present has blueberry, mulberry fruit, Grape Skin, blackcurrant, Qarnet rice, purple wild cabbage etc.The anthocyanidin that from mulberry fruit, extracts have content height, character more stable, receive advantages such as illumination and temperature effect is less, become the irreplaceable fresh fruit pigment of other fruits.
Through the retrieval of document and patent, also there is not report at present about Chinese caterpillar fungus cyanine oral liquid.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese caterpillar fungus cyanine oral liquid; This oral liquid is assembled the nutrient of cordyceps mycelia, Cordyceps militaris and anthocyanidin, not only has the effects such as smoothness that effects such as beneficial vital essence, guarantor's lung also have enhance immunity power, promote skin.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned oral liquid.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is following:
A kind of Chinese caterpillar fungus cyanine oral liquid, it comprises the component of following parts by weight:
10～20 parts of cordyceps mycelia extracts;
10～20 parts of anthocyanidin extracts;
2～5 parts of Cordyceps militaris concentrates.
Wherein, described cordyceps mycelia extract, Cordyceps sinensis polysaccharide content wherein is 5～10wt%.
Wherein, described cordyceps mycelia extract makes as follows: the cordyceps mycelia water is extracted, concentrate; Add ethanol in the concentrate, under 4 ℃, leave standstill more than the 24h, cross and filter the medicinal extract thin up; Filter, filtrating adds water and stirs, and under 4 ℃, continues to leave standstill more than the 24h; Remove by filter insoluble matter, concentrate through vacuum again and obtain the cordyceps mycelia extract.
Wherein, described anthocyanidin extract, anthocyanidin content wherein are 6～10wt%
Wherein, described anthocyanidin extract makes as follows: get fresh mulberries, smash to pieces, add pectase and amylase, utilize ultrasonic-assisted extraction simultaneously, extract elder generation vacuum filtration concentrates through vacuum again.
Wherein, described Cordyceps militaris concentrate, cordycepin content wherein are 1～2wt%.
Wherein, described Cordyceps militaris concentrate makes as follows: with the Chinese caterpillar fungus strain slant culture, cultivate and the fermented liquid cultivation through shake-flask seed liquid; Get silkworm chrysalis alive,, cultivate and obtained Cordyceps militaris in 35-50 days Chinese caterpillar fungus strain zymotic fluid inoculation silkworm chrysalis alive; Get Cordyceps militaris and add entry, grind on ice and process homogenate, add protease, utilize ultrasonic-assisted extraction simultaneously, extract elder generation vacuum filtration, vacuum concentrates again, removes by filter insoluble matter and obtains the Cordyceps militaris concentrate.
Chinese caterpillar fungus cyanine oral liquid of the present invention can add suitable honey and sucrose seasoning.
The preparation method of above-mentioned Chinese caterpillar fungus cyanine oral liquid comprises the steps:
(1) the cordyceps mycelia water is extracted, concentrate, add ethanol in the concentrate, under 4 ℃, leave standstill more than the 24h; Cross and filter the medicinal extract thin up, filter, filtrating adds water and stirs; Under 4 ℃, continue to leave standstill more than the 24h, remove by filter insoluble matter, concentrate through vacuum again and obtain the cordyceps mycelia extract;
(2) get fresh mulberries, smash to pieces, add pectase and amylase, utilize ultrasonic-assisted extraction simultaneously, extract elder generation vacuum filtration, again through vacuum concentrate the anthocyanidin extract;
(3), cultivate and the fermented liquid cultivation through shake-flask seed liquid with the Chinese caterpillar fungus strain slant culture; Get silkworm chrysalis alive,, cultivate and obtained Cordyceps militaris in 35-50 days Chinese caterpillar fungus strain zymotic fluid inoculation silkworm chrysalis alive; Get Cordyceps militaris and add entry, grind on ice and process homogenate, add protease, utilize ultrasonic-assisted extraction simultaneously, extract elder generation vacuum filtration, vacuum concentrates again, removes by filter insoluble matter and obtains the Cordyceps militaris concentrate;
(4) the cordyceps mycelia extract that step (1), (2), (3) is made respectively, anthocyanidin extract, Cordyceps militaris concentrate mix by formula ratio with honey, and can promptly gets after homogenizing.
Beneficial effect: the present invention compared with prior art has following advantage:
1, the present invention is harmonious according to all medicines, and the principle of synergy has been improved the not enough shortcoming of effect that Chinese caterpillar fungus and anthocyanidin use separately each other.The effects such as smoothness of utilize Chinese caterpillar fungus benefit vital essence, protect effects such as lung and anthocyanidin enhance immunity power, promoting skin combine, and function and skin glossiness in the human body machine are all improved, so reach from the inside to the outside, purpose that integral body is adjusted.
2, the Chinese caterpillar fungus cyanine oral liquid of the present invention preparation, have cost low, be of high nutritive value, advantage such as mouthfeel is good.
3, the present invention adopts the ultrasound wave auxiliary enzyme extraction method in Chinese caterpillar fungus and anthocyanidin leaching process, has removed impurity such as albumen and pectin in the time of extraction, has fully extracted the active component in the raw material; Reduced the viscosity of Chinese caterpillar fungus and anthocyanidin extract; Increase the clarity of oral liquid, reduced the sediment of oral liquid finished product, technology is simple; Effective, make the oral liquid good visual effect.
Description of drawings
Fig. 1 is the DPPH clearance curve.
Fig. 2 is the oral liquid bacteriostatic test plate.Wherein (A) suppresses rhizopus figure; (B) suppress staphylococcus aureus; A is blank (filter paper that water logging was steeped); B is the filter paper that oral liquid soaked.
The specific embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that the described content of embodiment only is used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Used Chinese caterpillar fungus strain (Cordyceps millitaris) ACCC50383 of the present invention is available from Chinese agriculture microorganism fungus kind preservation administrative center.
Embodiment 1
A kind of Chinese caterpillar fungus cyanine oral liquid comprises the component of following parts by weight:
(wherein, Cordyceps sinensis polysaccharide content 5～10wt%) for cordyceps mycelia extract 200g;
(wherein, anthocyanidin content 6～10wt%) for anthocyanidin extract 200g;
(wherein, cordycepin content 1～2wt%) for Cordyceps militaris concentrate 50g.
After the above-mentioned mixing of materials, water is settled to 1L.
The production technology of Chinese caterpillar fungus cyanine oral liquid:
(1) preparation of cordyceps mycelia extract: get the cordyceps mycelia water and extract, be concentrated in right amount, stir, add long-pending 95% (v/v) alcohol of dliploid; Put into 4 ℃ of refrigerations and leave standstill 24h, filter, reclaim ethanol, the medicinal extract adding distil water is diluted in right amount; Filter, add auxiliary materials (for example: sucrose, honey) such as flavor enhancement, stir, add water to capacity; Stir, 4 ℃ leave standstill 24h, remove by filter insoluble matter, concentrate through vacuum and obtain the cordyceps mycelia extract.
(2) preparation of anthocyanidin extract: get fresh mulberries → tissue mashing machine and smash → accurately take by weighing a certain amount of mulberries tissue → adding extraction enzyme (food is with 500～1000U/L pectase and 500～1000U/L amylase) reagent → ultrasonic-assisted extraction (100w extracts 1.5h) → extract vacuum filtration → vacuum concentrated extracting solution to pieces.
(2) preparation of Cordyceps militaris concentrate: get Chinese caterpillar fungus strain (Cordyceps millitaris) slant culture, shake-flask seed liquid is cultivated, and fermented liquid is cultivated.Get silkworm chrysalis alive,, cultivate and obtained Cordyceps militaris in 35-50 days Chinese caterpillar fungus strain zymotic fluid inoculation silkworm chrysalis alive.Get Cordyceps militaris and add entry; Homogenate is processed in grinding on ice; (food adds supersonic wave cleaning machine with reaction system and carries out ultrasonic-assisted extraction (100w extracts 1.5h), extract vacuum filtration with protease 500～1000U/L) reagent to add the extraction enzyme; The vacuum concentrated extracting solution removes by filter insoluble matter and obtains the Cordyceps militaris concentrate.
(4) preparation of Chinese caterpillar fungus cyanine oral liquid: cordyceps mycelia extract, anthocyanidin extract, Cordyceps militaris concentrate, honey and water are mixed according to weight proportion; Concentrate; Homogenize; Can; Capping; Sterilization; Packing; Check; Finished product.
Embodiment 2:
A kind of Chinese caterpillar fungus cyanine oral liquid comprises the component of following parts by weight:
(wherein, Cordyceps sinensis polysaccharide content 5～10wt%) for cordyceps mycelia extract 100g;
(wherein, anthocyanidin content 6～10wt%) for anthocyanidin extract 100g;
(wherein, cordycepin content 1～2wt%) for Cordyceps militaris concentrate 20g.
After the above-mentioned mixing of materials, water is settled to 1L.
The preparation method is with embodiment 1.
Embodiment 3:
A kind of Chinese caterpillar fungus cyanine oral liquid comprises the component of following parts by weight:
(wherein, Cordyceps sinensis polysaccharide content 5～10wt%) for cordyceps mycelia extract 150g;
(wherein, anthocyanidin content 6～10wt%) for anthocyanidin extract 150g;
(wherein, cordycepin content 1～2wt%) for Cordyceps militaris concentrate 40g.
After the above-mentioned mixing of materials, water is settled to 1L.
The preparation method is with embodiment 1.
Embodiment 4: the anti-oxidant experiment of oral liquid.
Remove organic free radical 1 through using, the anti-oxidant method of 1-diphenyl-2-trinitrophenyl-hydrazine (DPPH) is estimated the oxidation resistance of Chinese caterpillar fungus cyanine oral liquid.
Take by weighing 2.5mg DPPH and be settled to 100mL with absolute ethyl alcohol, concentration 25 μ g/mL place refrigerator to preserve, and are existing with join at present.Pipette 0.00mL, 2.00mL, 4.00mL, 6.00mL, 8.00mL, the above-mentioned standard liquid of 10.00mL is settled to 10mL, and absolute ethyl alcohol is blank, measures absorbance, drawing curve at the 515nm place.Sample thief oral liquid (embodiment 1) decompression concentrates 10 times, gets concentrate 0.1mL, and adding 3.9mL concentration is 25 μ g/mL [DPPH], shakes up, and the variation of 515nm wavelength absorbance in the 30min is measured in colour developing.Calculate the system dose rate.Data are as shown in Figure 1, and the result shows that interior this oral liquid of 30min is 42% for the clearance rate of DPPH, the stronger oxidation resistance that has.
Embodiment 5: the examination of oral liquid bacteriostasis.
The filter paper that oral liquid was soaked; The aseptic wind of room temperature (30 ℃) is done, and filter paper is added just be coated with on the solid plate culture medium of spoilage organisms (head mold) and pathogenic bacteria (staphylococcus aureus), and the filter paper that steeps with water logging is as contrast; The dull and stereotyped 3d that cultivates observes antibacterial situation.The result shows that (Fig. 2) oral liquid has the effect that suppresses mould rotten bacterium and pathogen.
Claims (8)
1. Chinese caterpillar fungus cyanine oral liquid is characterized in that it comprises the component of following parts by weight:
10～20 parts of cordyceps mycelia extracts;
10～20 parts of anthocyanidin extracts;
2～5 parts of Cordyceps militaris concentrates.
2. Chinese caterpillar fungus cyanine oral liquid according to claim 1 is characterized in that, described cordyceps mycelia extract, and Cordyceps sinensis polysaccharide content wherein is 5～10wt%.
3. Chinese caterpillar fungus cyanine oral liquid according to claim 1 and 2 is characterized in that, described cordyceps mycelia extract makes as follows: the cordyceps mycelia water is extracted; Concentrate, add ethanol in the concentrate, under 4 ℃, leave standstill more than the 24h; Cross and filter the medicinal extract thin up, filter, filtrating adds water and stirs; Under 4 ℃, continue to leave standstill more than the 24h, remove by filter insoluble matter, concentrate through vacuum again and obtain the cordyceps mycelia extract.
4. Chinese caterpillar fungus cyanine oral liquid according to claim 1 is characterized in that described anthocyanidin extract, anthocyanidin content wherein are 6～10wt%
5. according to claim 1 or 4 described Chinese caterpillar fungus cyanine oral liquids; It is characterized in that described anthocyanidin extract makes as follows: get fresh mulberries, smash to pieces; Add pectase and amylase; Utilize ultrasonic-assisted extraction simultaneously, extract elder generation vacuum filtration concentrates through vacuum and obtains the anthocyanidin extract.
6. Chinese caterpillar fungus cyanine oral liquid according to claim 1 is characterized in that described Cordyceps militaris concentrate, cordycepin content wherein are 1～2wt%.
7. according to claim 1 or 6 described Chinese caterpillar fungus cyanine oral liquids, it is characterized in that described Cordyceps militaris concentrate makes as follows:, cultivate and the fermented liquid cultivation through shake-flask seed liquid with the Chinese caterpillar fungus strain slant culture; Get silkworm chrysalis alive,, cultivate and obtained Cordyceps militaris in 35-50 days Chinese caterpillar fungus strain zymotic fluid inoculation silkworm chrysalis alive; Get Cordyceps militaris and add entry, grind on ice and process homogenate, add protease, utilize ultrasonic-assisted extraction simultaneously, extract elder generation vacuum filtration, vacuum concentrates again, removes by filter insoluble matter and obtains the Cordyceps militaris concentrate.
8. the preparation method of the described Chinese caterpillar fungus cyanine of claim 1 oral liquid is characterized in that this method comprises the steps:
(1) the cordyceps mycelia water is extracted, concentrate, add ethanol in the concentrate, under 4 ℃, leave standstill more than the 24h; Cross and filter the medicinal extract thin up, filter, filtrating adds water and stirs; Under 4 ℃, continue to leave standstill more than the 24h, remove by filter insoluble matter, concentrate through vacuum again and obtain the cordyceps mycelia extract;
(2) get fresh mulberries, smash to pieces, add pectase and amylase, utilize ultrasonic-assisted extraction simultaneously, extract elder generation vacuum filtration concentrates through vacuum and obtains the anthocyanidin extract;
(3), cultivate and the fermented liquid cultivation through shake-flask seed liquid with the Chinese caterpillar fungus strain slant culture; Get silkworm chrysalis alive,, cultivate and obtained Cordyceps militaris in 35-50 days Chinese caterpillar fungus strain zymotic fluid inoculation silkworm chrysalis alive; Get Cordyceps militaris and add entry, grind on ice and process homogenate, add protease, utilize ultrasonic-assisted extraction simultaneously, extract elder generation vacuum filtration, vacuum concentrates again, removes by filter insoluble matter and obtains the Cordyceps militaris concentrate;
(4) the cordyceps mycelia extract that step (1), (2), (3) is made respectively, anthocyanidin extract, Cordyceps militaris concentrate mix by formula ratio with honey, add water, and can promptly gets after homogenizing.
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| CN105851244A (en) * | 2016-05-25 | 2016-08-17 | 山西普德食品有限公司 | Cordyceps sinensis soybean milk and special cordyceps militaris concentrated liquor thereof |
| CN106235332A (en) * | 2016-07-29 | 2016-12-21 | 梁忠顺 | A kind of Hylocereus undatus health product and preparation method thereof |
| CN109452643A (en) * | 2018-12-27 | 2019-03-12 | 河北农业大学 | Antioxidant mulberry anthocyanin oral liquid and preparation method thereof |
| CN112244291A (en) * | 2020-10-16 | 2021-01-22 | 台州康晶生物科技有限公司 | Cordyceps militaris oral liquid and preparation method and application thereof |
| CN116711855A (en) * | 2023-07-03 | 2023-09-08 | 沈阳农业大学 | Selenium-enriched chromium cordyceps sinensis oral liquid with blood sugar reducing function and preparation method thereof |
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| CN116711855A (en) * | 2023-07-03 | 2023-09-08 | 沈阳农业大学 | Selenium-enriched chromium cordyceps sinensis oral liquid with blood sugar reducing function and preparation method thereof |
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Application publication date: 20121212 |