CN106479903A - A kind of manufacture method of live body glossy ganoderma dish garden - Google Patents
A kind of manufacture method of live body glossy ganoderma dish garden Download PDFInfo
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- CN106479903A CN106479903A CN201610900613.XA CN201610900613A CN106479903A CN 106479903 A CN106479903 A CN 106479903A CN 201610900613 A CN201610900613 A CN 201610900613A CN 106479903 A CN106479903 A CN 106479903A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The present invention relates to cultivation of glossy ganoderma technical field, a kind of specifically related to manufacture method of live body glossy ganoderma dish garden, including acquisition lucid ganoderma stock culture, Ganoderma original seed, cultivation of glossy ganoderma kind respectively, carry out substituting stuff cultivation, go out sesame, transplant to bonsai type container, periodically or non-periodically input nutritional solution and/or water in described bonsai type container；The culture medium of substituting stuff cultivation is identical with Cultivar culture medium；Wherein Cultivar culture medium includes wood flour, pig manure, cattle manure, rapeseed meal, humic acidss, Mycen osmundicola fermentation liquid, bacillus subtilis fermentation liquor, Gypsum Fibrosum.The present invention is remarkably improved Ganoderma sporophore yield, and also significantly extends the storage life of live body glossy ganoderma dish garden and improve its preservation quality.
Description
Technical field
The present invention relates to cultivation of glossy ganoderma technical field is and in particular to a kind of manufacture method of live body glossy ganoderma dish garden.
Background technology
Ganoderma (Ganoderma Lucidum Karst) is the sporophore of On Polyporaceae Ganoderma.Have invigorating QI and tranquilization,
Relieving cough and asthma, effect of life lengthening.For dizziness sleeplessness, shortness of breath and palpitation, neurasthenia, asthenia cough with asthma.Ganoderma is China doctor
Learn one of treasure-house rare Chinese medicine, be to integrate health care and the medicinal edible fungi as the whole body.Modern medicine study proves, in Ganoderma
Polysaccharide, triterpeness, sterols isoreactivity composition, have antitumor, adjust immunity, nervous system regulation, adjust circulation to human body
The function of system etc..Ganoderma, mild-natured, be strengthening by means of tonics, consolidate righting, the precious tonic of life lengthening.The ancient medicine of China
Treasured book《Sheng Nong's herbal classic》And Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》Described in Ganoderma：" add medicine to, main life of supporting is with Ying Tian.The beneficial motive, mends
In, increase intelligence, hard muscles and bones, good color, eat long and make light of one's life by commiting suicide." Ganoderma implied meaning is beautiful, lucky, rich and honour, long-lived, as one wishes.Sheng Nong's herbal classic
Record, " mountains and rivers sexual intercourse five elements' YIN YANG in four seasons essence round the clock, can give birth to multicolored Ganoderma, and all holy king stops auspicious easy "；Solid " the spirit of Han dynasty class
Sesame is sung " praise：" as three morals Ying Ruitu, life of lengthening the life and light longevity are all "；Wei is towards Cao Zhi " Ganoderma forever " Shi Zhongyezan road：" high honour phase
Shake credit, if honorable bright refreshing ".
Glossy ganoderma dish garden scene is attractive in appearance, simple and unsophisticated pure and fresh, and implied meaning is lucky rich and honour, has deep artistic connotation, has become as height
The first-selection of shelves decoration, is also present, the fashion good merchantable brand of collection.Produce glossy ganoderma dish garden and there is high economic benefit and society's effect
Benefit.
Content of the invention
It is an object of the present invention to provide a kind of manufacture method of live body glossy ganoderma dish garden.
Technical solution of the present invention is as follows：
A kind of manufacture method of live body glossy ganoderma dish garden, comprises the following steps：
1) lucid ganoderma stock culture is seeded to mother culture media, obtains lucid ganoderma stock culture；
Described mother culture media includes following component：Rhizoma Solani tuber osi 200-300g/L, glucose 20-30g/L, Carnis Bovis seu Bubali cream 3-
5g/L, peptone 4-10g/L, agar powder 15-20g/L；
2) lucid ganoderma stock culture is seeded to pedigree seed culture medium culture, obtains Ganoderma original seed；
Described pedigree seed culture medium includes the composition of following weight portion：Wood flour 60-80 part, corn cob 5-10 part, Testa Tritici 10-15
Part, brown sugar 1-3 part, Gypsum Fibrosum powder 1-3 part, KNO30.05-0.5 part；Described pedigree seed culture medium water content is 55-60%；
3) Ganoderma original seed is seeded to culture in Cultivar culture medium, obtains cultivation of glossy ganoderma kind；
Described Cultivar culture medium includes the raw material of following weight portion：Wood flour 30-80 part, pig manure 5-15 part, cattle manure 5-15,
Rapeseed meal 5-25 part, humic acidss 5-10 part, Mycen osmundicola (Mycena osmundicola Lange) fermentation liquid 1-10 part, hay
Bacillus cereuss (Bacillus subtilis) fermentation liquid 1-10 part, Gypsum Fibrosum 1-3 part；Effectively alive in described Mycen osmundicola fermentation liquid
Bacterium number is 1.0-5.0 × 106/ mL, in described bacillus subtilis fermentation liquor, living bacteria count is 1.0-1.5 × 1010/mL；Institute
The preparation method stating Cultivar culture medium comprises the following steps：By proportioning, each raw material is mixed, adds water to water content 50-70%,
Ferment to becoming thoroughly decomposed；Adjust moisture to 55-60%, sterilize, you can；
4) described cultivation of glossy ganoderma kind is carried out substituting stuff cultivation, go out sesame；The culture medium of described substituting stuff cultivation is cultivated with cultigen
Base is identical；
5) by step 4) gained Ganoderma transplants to bonsai type container, periodically or non-periodically to defeated in described bonsai type container
Enter nutritional solution and/or water；
Substrate in described bonsai type container includes the raw material of following weight portion：Sandy loam 80-100 part, sawdust 10-30
Part, plant ash 1-3 part, Gypsum Fibrosum powder 1-3 part；
Described bonsai type container and described substrate are sterilized in advance or disinfect, and thoroughly remove microorganism and moth or worm
Ovum；
Nutritional solution described in every 1kg includes following component：Cultivar culture medium leachate 50-100mL, dehydroactic acid sodium 500-
1000mg, MgSO4100-500mg, KNO3100-200mg, K2HPO450-100mg, Triiodobenzoic acid 100-200mg,
EDTA-2Na (i.e. disodiumedetate) 5-10mg, vitamin B15-10mg, water (solvent)；
Described Cultivar culture medium leaches liquid and preparation method thereof and includes described Cultivar culture medium adds the water of 10 times of weight
Soak 10-24h, filter, filtrate is sterilized, you can.
Preferably, described mother culture media includes following component：Rhizoma Solani tuber osi 250g/L, glucose 25g/L, Carnis Bovis seu Bubali cream 3g/
L, peptone 6g/L, agar powder 15-20g/L.
Preferably, described Mother culture condition includes：Constant temperature lucifuge culture at 22-26 DEG C；General culture 5-7 days
Mycelia covers with medium slant.
Preferably, described pedigree seed culture medium includes the composition of following weight portion：70 parts of wood flour, 6 parts of corn cob, Testa Tritici 15
Part, 2 parts of brown sugar, 2 parts of Gypsum Fibrosum powder, KNO30.25 part；Described pedigree seed culture medium water content is 55-60%.
Preferably, described Primary spawn condition includes：Temperature be 20-22 DEG C, humidity be 55-65% under constant temperature lucifuge training
Support, general culture 35-40 days.
Preferably, described Cultivar culture medium or generation material culture medium, including the raw material of following weight portion：40 parts of wood flour, pig
5 parts of excrement, 15 parts of cattle manure, 15 parts of rapeseed meal, 6 parts of humic acidss, 5 parts of Mycen osmundicola fermentation liquid, 5 parts of bacillus subtilis fermentation liquor,
2 parts of Gypsum Fibrosum；In described Mycen osmundicola fermentation liquid, living bacteria count is 1.0-3.0 × 106/ mL, described fermentation of bacillus subtilis
In liquid, living bacteria count is 1.0-1.2 × 1010/mL.
Preferably, described cultigen condition of culture includes：Temperature be 20-22 DEG C, humidity be 55-65% under constant temperature training
Support, general culture 35-50 days；Lucifuge culture in early stage 10 days, later stage intensity of illumination is 1000-1500 1x (lux)；Favorably
In mycelia fast-growth.
Mother culture media of the present invention, pedigree seed culture medium all can be obtained by this area conventional method.
Described substituting stuff cultivation goes out sesame incubation time and is generally 30-50d.
Preferably, described substituting stuff cultivation condition (going out sesame condition) includes：Cultivation temperature is 28-30 DEG C, and air is relatively wet
Spend for 60-75%, lucifuge culture in early stage 10 days, later stage intensity of illumination is 1000-1500 1x (lux)；Be conducive to mycelia
Fast-growth.
Preferably, described go out sesame be finalize sesame, go out sesame including artificial set point control or manually finalize sesame, all can be by normal
Rule method is carried out.
Preferably, step 5) member-retaining portion substituting stuff cultivation culture medium when transplanting Ganoderma, to guarantee that Ganoderma transplant survival is defined.
Preferably, the substrate in described bonsai type container includes the raw material of following weight portion：85 parts of sandy loam, sawdust 12
Part, 2 parts of plant ash, 2 parts of Gypsum Fibrosum powder；
Preferably, nutritional solution described in every 1kg includes following component：Cultivar culture medium leachate 80mL, dehydroactic acid sodium
600mg, MgSO4300mg, KNO3150mg, K2HPO4100mg, Triiodobenzoic acid 180mg, EDTA-2Na 10mg, vitamin
B110mg, water (solvent)；
Described nutritional solution includes mixing each raw material in proportion according to a conventional method.Preferably, described nutritional solution is entered
Row sterilization treatment, putrid and deteriorated to prevent.
The present invention also provides the substrate in a kind of bonsai type container making for live body glossy ganoderma dish garden, and described bonsai type holds
Substrate in device includes the raw material of following weight portion：Sandy loam 80-100 part, sawdust 10-30 part, plant ash 1-3 part, Gypsum Fibrosum powder
1-3 part；Preferably, the substrate in described bonsai type container includes the raw material of following weight portion：85 parts of sandy loam, 12 parts of sawdust,
2 parts of plant ash, 2 parts of Gypsum Fibrosum powder.
Sandy loam of the present invention is with general sandy loam.
The present invention also provides a kind of nutritional solution for the making of live body glossy ganoderma dish garden (to be mainly used in inputting in bonsai type container
Substrate in), nutritional solution described in every 1kg includes following component：Cultivar culture medium leachate 50-100mL, dehydroactic acid sodium
500-1000mg, MgSO4100-500mg, KNO3100-200mg, K2HPO450-100mg, Triiodobenzoic acid 100-200mg,
EDTA-2Na5-10mg, vitamin B15-10mg, water (solvent)；Preferably, nutritional solution described in every 1kg includes following component：Plant
Cultivate culture medium leachate 80mL, dehydroactic acid sodium 600mg, MgSO4300mg, KNO3150mg, K2HPO4100mg, triiodo-benzene
Formic acid 180mg, EDTA-2Na 10mg, vitamin B110mg, water (solvent).
The present invention also provides a kind of Cultivar culture medium of cultivating ganoderma or generation material culture medium, former including following weight portion
Material：Wood flour 30-50 part, pig manure 5-15 part, cattle manure 5-15 part, rapeseed meal 5-25 part, humic acidss 5-10 part, Mycen osmundicola
(Mycenaosmundicola Lange) zymotic fluid 1-10 part, bacillus subtilises (Bacillus subtilis) fermentation liquid 1-10
Part, Gypsum Fibrosum 1-3 part；In described Mycen osmundicola fermentation liquid, living bacteria count is 1.0-5.0 × 106/ mL, described bacillus subtilises
In fermentation liquid, living bacteria count is 1.0-1.5 × 1010/mL；The preparation method of described Cultivar culture medium comprises the following steps：Press
Each raw material is mixed by proportioning, adds water to water content 50-70%, ferments to becoming thoroughly decomposed；Adjust moisture to 55-60%, sterilize, that is,
Can.
Preferably, described Cultivar culture medium or generation material culture medium, including the raw material of following weight portion：40 parts of wood flour, pig
5 parts of excrement, 15 parts of cattle manure, 15 parts of rapeseed meal, 6 parts of humic acidss, 5 parts of Mycen osmundicola fermentation liquid, 5 parts of bacillus subtilis fermentation liquor,
2 parts of Gypsum Fibrosum；In described Mycen osmundicola fermentation liquid, living bacteria count is 1.0-3.0 × 106/ mL, described fermentation of bacillus subtilis
In liquid, living bacteria count is 1.0-1.2 × 1010/mL.
Described humic acidss are commercially available to be buied, for example, be purchased from Shandong Creation Hhumic Acid Science and Technology Co., Ltd.
Described bacillus subtilises are preferably CGMCC 1.3358.
Mycen osmundicola of the present invention, bacillus subtilises all can pass through commercially available, for example be purchased from China commonly micro-
Biological inoculum collection.
Sterilizing described in the preparation method of Cultivar culture medium or generation material culture medium needs thoroughly to inactivate bacillus subtilises
And its various microorganisms such as spore, this area conventional sterilization procedures can be adopted.
Described Mycen osmundicola fermentation liquid preparation can adopt this area conventional method, and such as preparation method includes taking Mycen osmundicola
Parent species, are inoculated in culture fluid, shaking table culture 5-10d, filter Mycen osmundicola fungus ball with gauze, obtain final product Mycen osmundicola fermentation liquid；Can
It is placed in refrigerator and preserve.The preferred 140-160rpm shaken cultivation of described shaking table culture rotating speed, preferred 22-26 DEG C of cultivation temperature；One
As bottled Culture liquid measure 125-150mL of 250mL triangle, bottled Culture liquid measure 250-300mL of 500mL triangle.
Described bacillus subtilis fermentation liquor preparation can adopt this area conventional method, for example, comprise the following steps：
1) slant culture：The original strain of bacillus subtilises is aseptically inoculated on slant medium,
36-48h is cultivated under the conditions of 29 ± 1 DEG C；The formula of slant medium is as follows：Glucose 15g, peptone 5g, yeast extract 5g, water
1000mL, agar 15g；
2) shaking table culture：By step 1) strain that obtains of culture is inoculated in fluid medium, in pH 6.5-7.0, temperature be
Under the conditions of 30 ± 2 DEG C, 140-160r/min shaking table culture 36-48h；Liquid culture based formulas are as follows：Semen Maydis powder 13g, glucose
5g, soybean cake powder 20g, fish flour 5g, CaCO32g, (NH4)2SO41g, K2HPO40.3g, MgSO4·7H2O 0.2g, MnSO4·
H2O0.2g, water 1000ml；
3) fermentor cultivation：By step 2) strain that obtains of culture is inoculated in fermentation tank culture medium, in pH 7.5-8.0, tank
Pressure 0.5kg, temperature are 30 ± 2 DEG C, ventilation 1:Under the conditions of 0.8-1.1, it is more than 1.0-1.2 × 10 to bacterium number10/ mL tank at present,
Obtain final product bacillus subtilis fermentation liquor；Fermentor cultivation based formulas are as follows：Semen Maydis powder 5kg, soybean cake powder 2.5kg, ammonium sulfate
0.5kg, glucose 1.5kg, yeast powder 0.5kg, peptone 0.25kg, add water to 100kg；It is generally incubated 48-56h.
Specifically, Cultivar culture medium or generation material culture medium preparation method described in ferment to become thoroughly decomposed including：Fermentation heap
First time turning when interior temperature reaches 50 DEG C, turning daily 1-2 time later, and supplement amount of water, treat that fermentation heap temperature reduces
To less than 35 DEG C, moisture be down to less than 30% end fermentation.
The inventive method is applied to various Ganodermas, is particularly suited for Ganoderma Ganoderma lucidum (Leyss.:Fr.)
Karst；Ganoderma Ganoderma sinense J.D.Zhao, L.W.Hsu et X.Q.Zhang；Ganoderma capenseD.A.Reid Ganoderma
capense(L loyd)D.A.Reid.
All commercially available the buying of Ganderma lucidum strain of the present invention obtains it is also possible to separate from wild Ganoderma sporophore.
Present invention optimizes mother culture media, pedigree seed culture medium, Cultivar culture medium, make Mycelium Growth of Ganoderma lucidum faster, more
The later stage is conducive to form sporophore.Especially, Cultivar culture medium of the present invention is remarkably improved Ganoderma sporophore yield.The present invention
First Mycen osmundicola is applied in the rotten fermentation of cultivation of glossy ganoderma kind culture medium heap, obtained Cultivar culture medium (also is used as
Generation material culture medium) possess bacteria resistance function, significantly reduce bacterial contamination rate, improve Ganoderma quality, increased economic benefit.This
Inventive method is simple and easy to do, invests little, instant effect it is easy to promote；The inventive method mycelial growth rate is fast, and mycelia daily grows
, up to 18-20mm/d, up to 9%-15%, fruiting body yield is high, quality is good, significantly improves for sporophore biological efficiency for speed
Economic benefit.The present invention also optimizes bonsai type container substrate and nutritional solution.The inventive method significantly extends live body Ganoderma
The storage life of potted landscape and improve its preservation quality.The glossy ganoderma dish garden that the inventive method makes is live body Ganoderma, and experiment proves
Still keep condition of living organism through depositing Ganoderma after 3 years, the glossy ganoderma dish garden of the phenomenon that occurs in 3 years damaging by worms is less than 10%.
Specific embodiment
Following examples are used for the present invention is described, but are not limited to the scope of the present invention.Unreceipted concrete in embodiment
Technology or condition person, according to the technology described by document in the art or condition, or are carried out according to product description.Used
Reagent or the unreceipted production firm person of instrument, are the conventional products being commercially available by regular distributor.
Strain of the present invention all can pass through commercially available.Described below bacillus subtilises are CGMCC 1.3358.
Live body Ganoderma experimental technique includes：To be cut in Ganoderma sporophore in glossy ganoderma dish garden with sterile scalpel, with solution
Cut open cutter cuts 0.2-0.5cm blockage tissue, with tweezers, this tissue is placed in culture dish PDA culture medium and (includes following component：Horse
Bell potato 200g/L, glucose 20g/L, agar powder 15g/L) central authorities, culture 2-3d after visible white hypha is grown by piece of tissue,
Prove live body Ganoderma.Continue the Ganoderma mycelium that tube can obtain purification for 1-2 time, still can obtain Ganoderma by the inventive method real
Body.
Embodiment 1
A kind of manufacture method of live body glossy ganoderma dish garden, comprises the following steps：
1) by Ganoderma Ganoderma lucidum (Leyss.:Fr.) Karst parent species are seeded to mother culture media in 22-26
Constant temperature culture 5-7 days at DEG C, obtains lucid ganoderma stock culture.Described mother culture media includes following component：Rhizoma Solani tuber osi 250g/L, glucose
25g/L, Carnis Bovis seu Bubali cream 3g/L, peptone 6g/L, agar powder 15-20g/L.
2) lucid ganoderma stock culture is seeded to pedigree seed culture medium to cultivate under temperature 20-22 DEG C, humidity 55-65%, obtains Ganoderma
Original seed.Described pedigree seed culture medium includes the composition of following weight portion：70 parts of wood flour, 6 parts of corn cob, 15 parts of Testa Tritici, 2 parts of brown sugar,
2 parts of Gypsum Fibrosum powder, KNO30.25 part；Described pedigree seed culture medium water content is 55-60%；Pedigree seed culture medium makes 500mL original seed
Bottle.The preparation method of described pedigree seed culture medium includes taking each raw material by proportioning, adjusts moisture, sterilizing after mixing.
Described Mother culture, Primary spawn condition are all cultivated in darkroom lucifuge.
3) Ganoderma original seed is seeded to culture in the Cultivar culture medium in cultivating bag, obtains cultivation of glossy ganoderma kind.Described cultivation
Cultivate condition of culture include temperature be 20-22 DEG C, humidity be constant temperature culture under 55-65%, general culture 35-50 days；Early stage
Lucifuge culture in 10 days, later stage intensity of illumination is 1000-1500 1x (lux).
Described Cultivar culture medium includes the raw material of following weight portion：40 parts of wood flour, 5 parts of pig manure, 15 parts of cattle manure, rapeseed meal
15 parts, 6 parts of humic acidss, 5 parts of Mycen osmundicola fermentation liquid, 5 parts of bacillus subtilis fermentation liquor, 2 parts of Gypsum Fibrosum；Described Mycen osmundicola is sent out
In zymotic fluid, living bacteria count is 1.0-3.0 × 106/ mL, in described bacillus subtilis fermentation liquor, living bacteria count is 1.0-1.2
×1010/mL.Described Mycen osmundicola zymotic fluid preparation method includes taking Mycen osmundicola parent species, is inoculated in culture fluid, shaking table 140rpm
Culture 7-8d, cultivation temperature 22-26 DEG C, filter Mycen osmundicola fungus ball with gauze, obtain final product Mycen osmundicola fermentation liquid.
The preparation method of described Cultivar culture medium comprises the following steps：By proportioning, each raw material is mixed, add water to aqueous
Amount 50-70%, first time turning when temperature reaches 50 DEG C in fermentation heap, turning daily 1-2 time later, and supplement amount of water,
Treat fermentation heap temperature be reduced to less than 35 DEG C, moisture be down to less than 30% and show fermentation maturity, terminate fermentation；Adjust moisture
To 55-60%, thoroughly sterilize content (thoroughly inactivateing the various microorganism such as bacillus subtilises and its spore), you can.
4) described cultivation of glossy ganoderma kind is carried out substituting stuff cultivation and go out sesame culture, carry out artificial set point control according to a conventional method and go out sesame
Or manually finalize sesame, obtain Ganoderma.Described generation material culture medium is identical with Cultivar culture medium.
Described substituting stuff cultivation condition includes：Cultivation temperature is 28-30 DEG C, and relative air humidity is 60-75%, early stage 10 days
Interior lucifuge culture, later stage intensity of illumination is 1000-1500 1x (lux).
5) by step 4) gained Ganoderma transplants to bonsai type container, periodically or non-periodically to defeated in described bonsai type container
Enter nutritional solution and/or water；Member-retaining portion substituting stuff cultivation culture medium when transplanting Ganoderma, to guarantee that Ganoderma transplant survival is defined.
Substrate in described bonsai type container includes the raw material of following weight portion：85 parts of sandy loam, 12 parts of sawdust, plant ash
2 parts, 2 parts of Gypsum Fibrosum powder；
Described bonsai type container and described substrate are sterilized in advance or disinfect, and thoroughly remove microorganism and moth or worm
Ovum；
Nutritional solution described in every 1kg includes following component：Cultivar culture medium leachate 80mL, dehydroactic acid sodium 600mg,
MgSO4300mg, KNO3150mg, K2HPO4100mg, Triiodobenzoic acid 180mg, EDTA-2Na 10mg, vitamin B110mg,
Balance of water (solvent).
Described Cultivar culture medium leaches liquid and preparation method thereof and includes described Cultivar culture medium adds the water of 10 times of weight
Soak 24h, filter, filtrate is sterilized, you can.
Described nutritional solution includes mixing each raw material in proportion according to a conventional method, sterilizes.
Parent species, original seed, cultigen, the average daily speed of growth of cultivating bag mycelia are all up to 18-20mm/d.From former base to sporophore
Maturation typically needs 30-45d, can harvest within 1.5-2 month after general culture；Done in terms of generation material dry weight by 1000kg, can adopt then
Receive Ganoderma sporophore 90-125kg.(in terms of dry weight, the yield herein calculating only refers to step 4 gained Ganoderma yield to fruiting body yield,
Similarly hereinafter.) the present embodiment make glossy ganoderma dish garden be live body Ganoderma, experiment prove still keep condition of living organism through depositing Ganoderma after 3 years；
The glossy ganoderma dish garden of phenomenon of occurring in 3 years damaging by worms is less than 10%.
Embodiment 2
A kind of manufacture method of live body glossy ganoderma dish garden, with differing only in of embodiment 1：Described mother culture media include with
Lower composition：Rhizoma Solani tuber osi 200g/L, glucose 20g/L, Carnis Bovis seu Bubali cream 3g/L, peptone 4g/L, agar powder 15g/L；Described original seed training
Foster base includes the composition of following weight portion：60 parts of wood flour, 5 parts of corn cob, 10 parts of Testa Tritici, 1 part of brown sugar, 1 part of Gypsum Fibrosum powder, KNO3
0.05 part；Described pedigree seed culture medium water content is 55-60%；Described Cultivar culture medium includes the raw material of following weight portion：Wood
30 parts of bits, 5 parts of pig manure, cattle manure 10,5 parts of rapeseed meal, 5 parts of humic acidss, 1 part of Mycen osmundicola fermentation liquid, fermentation of bacillus subtilis
1 part of liquid, 1 part of Gypsum Fibrosum；In described Mycen osmundicola fermentation liquid, living bacteria count is 1.0-2.0 × 106/ mL, described bacillus subtilis
In fermented liquid, living bacteria count is 1.0-1.2 × 1010/mL.Substrate in described bonsai type container includes following weight portion
Raw material：80 parts of sandy loam, 10 parts of sawdust, 1 part of plant ash, 1 part of Gypsum Fibrosum powder；Nutritional solution described in every 1kg includes following component：Cultivation
Plant culture medium leachate 50mL, dehydroactic acid sodium 500mg, MgSO4100mg, KNO3100mg, K2HPO450mg, triiodo-benzene first
Sour 100mg, EDTA-2Na 5mg, vitamin B15mg, balance of water (solvent).
Parent species, original seed, cultigen, the average daily speed of growth of cultivating bag mycelia are all up to 18-20mm/d.From former base to sporophore
Maturation typically needs 30-45d, can harvest within 1.5-2 month after general culture；Done in terms of generation material dry weight by 1000kg, can adopt then
Receive Ganoderma sporophore 90-120kg.The glossy ganoderma dish garden that the present embodiment makes is live body Ganoderma, and experiment proves through depositing Ganoderma after 3 years
Still keep condition of living organism.The glossy ganoderma dish garden of phenomenon of occurring in 3 years damaging by worms is less than 10%.
Embodiment 3
A kind of manufacture method of live body glossy ganoderma dish garden, with differing only in of embodiment 1：Described mother culture media include with
Lower composition：Rhizoma Solani tuber osi 300g/L, glucose 30g/L, Carnis Bovis seu Bubali cream 5g/L, peptone 10g/L, agar powder 20g/L；Described original seed training
Foster base includes the composition of following weight portion：80 parts of wood flour, 10 parts of corn cob, 15 parts of Testa Tritici, 3 parts of brown sugar, 3 parts of Gypsum Fibrosum powder, KNO3
0.3 part；Described pedigree seed culture medium water content is 55-60%；Described Cultivar culture medium includes the raw material of following weight portion：Wood flour
50 parts, 15 parts of pig manure, 5 parts of cattle manure, 25 parts of rapeseed meal, 10 parts of humic acidss, 10 parts of Mycen osmundicola fermentation liquid, bacillus subtilises send out
10 parts of zymotic fluid, 3 parts of Gypsum Fibrosum；In described Mycen osmundicola fermentation liquid, living bacteria count is 1.0-2.0 × 106/ mL, described hay spore
In bacillus fermentation liquid, living bacteria count is 1.0-1.2 × 1010/mL.Substrate in described bonsai type container includes following weight portion
Raw material：100 parts of sandy loam, 30 parts of sawdust, 3 parts of plant ash, 3 parts of Gypsum Fibrosum powder；Nutritional solution described in every 1kg includes following component：
Cultivar culture medium leachate 100mL, dehydroactic acid sodium 1000mg, MgSO4500mg, KNO3200mg, K2HPO4100mg, three
Iodo-benzoic acid 200mg, EDTA-2Na 10mg, vitamin B110mg, balance of water (solvent).
Parent species, original seed, cultigen, the average daily speed of growth of cultivating bag mycelia are all up to 18-20mm/d.From former base to sporophore
Maturation typically needs 30-45d, can harvest within 1.5-2 month after general culture；Done in terms of generation material dry weight by 1000kg, can adopt then
Receive Ganoderma sporophore 90-120kg.The glossy ganoderma dish garden that the present embodiment makes is live body Ganoderma, and experiment proves through depositing Ganoderma after 3 years
Still keep condition of living organism.The glossy ganoderma dish garden of phenomenon of occurring in 3 years damaging by worms is less than 10%.
Comparative example 1
A kind of manufacture method of live body glossy ganoderma dish garden, with differing only in of embodiment 1：Mother culture media adopts PDA to train
Foster base, including following component：Rhizoma Solani tuber osi 200g/L, glucose 20g/L, agar powder 15g/L；Pedigree seed culture medium includes following weight
The composition of part：80 parts of hardwood sawdust, 18 parts of Testa Tritici, 1 part of sucrose, 1 part of Gypsum Fibrosum powder, add water after stirring, final culture medium
Water content is 55-60%；Described Mother culture, Primary spawn are 22 DEG C -25 DEG C constant temperature darkroom lucifuge cultures；Described cultigen
Culture medium is identical with substituting stuff cultivation culture medium, including the composition of following weight portion：73 parts of weed tree sawdust, 25 parts of Testa Tritici, analysis for soybean powder 1
Part, 1 part of Gypsum Fibrosum powder.Described substituting stuff cultivation condition includes：Cultivation temperature is 25-26 DEG C, and relative air humidity is 60-95%, front
20d intensity of illumination 1000-1500 lux, later stage intensity of illumination 1500-3000 lux.The glossy ganoderma dish garden made is only regular
Or irregularly in described bonsai type container, input water, but do not input any nutritional solution.
Parent species, original seed, cultigen, the general 10-15mm/d of the average daily speed of growth of cultivating bag mycelia.Become from former base to sporophore
Ripe typically need 40-50d, general cultivate after can harvest within 2-2.5 month；Done in terms of generation material dry weight by 1000kg, can harvest then
Ganoderma sporophore 40-50kg.The glossy ganoderma dish garden of phenomenon of occurring in 1 year damaging by worms is more than 30%.
Comparative example 2
A kind of manufacture method of live body glossy ganoderma dish garden, with differing only in of embodiment 1：Mother culture media adopts PDA to train
Foster base, including following component：Rhizoma Solani tuber osi 200g/L, glucose 20g/L, agar powder 15g/L；Pedigree seed culture medium includes following weight
The composition of part：80 parts of wood flour, 6 parts of corn cob, 18 parts of Testa Tritici, 2 parts of Gypsum Fibrosum powder；Add water after stirring, final culture medium is aqueous
Measure as 55-60%；Described Mother culture, Primary spawn are 22 DEG C -25 DEG C constant temperature darkroom lucifuge cultures.Described cultigen culture
Base is identical with substituting stuff cultivation culture medium, including the composition of following weight portion：78 parts of cotton seed hullss, 20 parts of Testa Tritici, 1 part of sucrose, Gypsum Fibrosum 1
Part；Described substituting stuff cultivation condition includes：Cultivation temperature is 25-26 DEG C, and relative air humidity is 60-95%, front 20d intensity of illumination
1000-1500 lux, later stage intensity of illumination 1500-3000 lux.The glossy ganoderma dish garden made only periodically or non-periodically to
Input water in described bonsai type container, but do not input any nutritional solution.
Parent species, original seed, cultigen, the general 10-15mm/d of the average daily speed of growth of cultivating bag mycelia.Become from former base to sporophore
Ripe typically need 40-50d, general cultivate after can harvest within 2-2.5 month；Done in terms of generation material dry weight by 1000kg, can harvest then
Ganoderma sporophore 40-50kg.The glossy ganoderma dish garden of phenomenon of occurring in 1 year damaging by worms is more than 30%.
Comparative example 3
A kind of manufacture method of live body glossy ganoderma dish garden, with differing only in of embodiment 1：Mother culture media adopts PDA to train
Foster base, including following component：Rhizoma Solani tuber osi 200g/L, glucose 20g/L, agar powder 15g/L；Pedigree seed culture medium includes following weight
The composition of part：80 parts of wood flour, 6 parts of corn cob, 18 parts of Testa Tritici, 2 parts of Gypsum Fibrosum powder；Add water after stirring, final culture medium is aqueous
Measure as 55-60%；Described Mother culture, Primary spawn are 22 DEG C -25 DEG C constant temperature darkroom lucifuge cultures.Described cultigen culture
Base is identical with substituting stuff cultivation culture medium, including the composition of following weight portion：43 parts of cotton seed hullss, 42 parts of weed tree sawdust, 10 parts of Testa Tritici, bean
3 parts of cake powder, 1 part of calcium superphosphate, 1 part of Gypsum Fibrosum.Described substituting stuff cultivation condition includes：Cultivation temperature is 25-26 DEG C, and air is relatively wet
Spend for 60-95%, front 20d intensity of illumination 1000-1500 lux, later stage intensity of illumination 1500-3000 lux.Make
Glossy ganoderma dish garden only periodically or non-periodically inputs water in described bonsai type container, but does not input any nutritional solution.
Parent species, original seed, cultigen, the general 10-15mm/d of the average daily speed of growth of cultivating bag mycelia.Become from former base to sporophore
Ripe typically need 40-50d, general cultivate after can harvest within 2-2.5 month；Done in terms of generation material dry weight by 1000kg, can harvest then
Ganoderma sporophore 40-50kg.The glossy ganoderma dish garden of phenomenon of occurring in 1 year damaging by worms is more than 30%.
Comparative example 4
A kind of manufacture method of live body glossy ganoderma dish garden, with differing only in of embodiment 1：Described Cultivar culture medium and generation
The raw material of material culture medium does not include Mycen osmundicola fermentation liquid and bacillus subtilis fermentation liquor.
Parent species, original seed, cultigen, the general 13-16mm/d of the average daily speed of growth of cultivating bag mycelia.Become from former base to sporophore
Ripe typically need 40-45d, general cultivate after can harvest within 2-2.5 month；Done in terms of generation material dry weight by 1000kg, can harvest then
Ganoderma sporophore 50-60kg.The glossy ganoderma dish garden of phenomenon of occurring in 1 year damaging by worms is more than 25%.
Although, above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (9)
1. a kind of manufacture method of live body glossy ganoderma dish garden is it is characterised in that comprise the following steps：
1) lucid ganoderma stock culture is seeded to mother culture media, obtains lucid ganoderma stock culture；
Described mother culture media includes following component：Rhizoma Solani tuber osi 200-300g/L, glucose 20-30g/L, Carnis Bovis seu Bubali cream 3-5g/L,
Peptone 4-10g/L, agar powder 15-20g/L；
2) lucid ganoderma stock culture is seeded to pedigree seed culture medium culture, obtains Ganoderma original seed；
Described pedigree seed culture medium includes the composition of following weight portion：Wood flour 60-80 part, corn cob 5-10 part, Testa Tritici 10-15 part,
Brown sugar 1-3 part, Gypsum Fibrosum powder 1-3 part, KNO30.05-0.5 part；Described pedigree seed culture medium water content is 55-60%；
3) Ganoderma original seed is seeded to culture in Cultivar culture medium, obtains cultivation of glossy ganoderma kind；
Described Cultivar culture medium includes the raw material of following weight portion：Wood flour 30-50 part, pig manure 5-15 part, cattle manure 5-15 part, dish
Seed dregs of rice 5-25 part, humic acidss 5-10 part, Mycen osmundicola fermentation liquid 1-10 part, bacillus subtilis fermentation liquor 1-10 part, Gypsum Fibrosum 1-3
Part；In described Mycen osmundicola fermentation liquid, living bacteria count is 1.0-5.0 × 106/ mL, has in described bacillus subtilis fermentation liquor
Effect viable count is 1.0-1.5 × 1010/mL；The preparation method of described Cultivar culture medium comprises the following steps：Will be each former by proportioning
Material mixing, adds water to water content 50-70%, ferments to becoming thoroughly decomposed；Adjust moisture to 55-60%, sterilize, you can；
4) described cultivation of glossy ganoderma kind is carried out substituting stuff cultivation, go out sesame；The culture medium of described substituting stuff cultivation and Cultivar culture medium phase
With；
5) by step 4) gained Ganoderma transplants to bonsai type container, the periodically or non-periodically input battalion in described bonsai type container
Nutrient solution and/or water；
Substrate in described bonsai type container includes the raw material of following weight portion：Sandy loam 80-100 part, sawdust 10-30 part, grass
Wood ash 1-3 part, Gypsum Fibrosum powder 1-3 part；
Described bonsai type container and described substrate are sterilized in advance or disinfect, and thoroughly remove microorganism and moth or worm's ovum；
Nutritional solution described in every 1kg includes following component：Cultivar culture medium leachate 50-100mL, dehydroactic acid sodium 500-
1000mg, MgSO4100-500mg, KNO3100-200mg, K2HPO450-100mg, Triiodobenzoic acid 100-200mg,
EDTA-2Na5-10mg, vitamin B15-10mg, water；
Described Cultivar culture medium leaches liquid and preparation method thereof and includes described Cultivar culture medium adds the water immersion of 10 times of weight
10-24h, filters, filtrate is sterilized, you can.
2. manufacture method according to claim 1 is it is characterised in that described mother culture media includes following component：Ma Ling
Potato 250g/L, glucose 25g/L, Carnis Bovis seu Bubali cream 3g/L, peptone 6g/L, agar powder 15-20g/L；And/or,
Described pedigree seed culture medium includes the composition of following weight portion：70 parts of wood flour, 6 parts of corn cob, 15 parts of Testa Tritici, 2 parts of brown sugar, stone
2 parts of cream powder, KNO30.25 part；Described pedigree seed culture medium water content is 55-60%；And/or,
Described Cultivar culture medium, generation material culture medium, including the raw material of following weight portion：40 parts of wood flour, 5 parts of pig manure, cattle manure 15
Part, 15 parts of rapeseed meal, 6 parts of humic acidss, 5 parts of Mycen osmundicola fermentation liquid, 5 parts of bacillus subtilis fermentation liquor, 2 parts of Gypsum Fibrosum；Described
In Mycen osmundicola fermentation liquid, living bacteria count is 1.0-3.0 × 106/ mL, effective viable bacteria in described bacillus subtilis fermentation liquor
Number is 1.0-1.2 × 1010/mL；And/or,
Substrate in described bonsai type container includes the raw material of following weight portion：85 parts of sandy loam, 12 parts of sawdust, 2 parts of plant ash,
2 parts of Gypsum Fibrosum powder；And/or,
Nutritional solution described in every 1kg includes following component：Cultivar culture medium leachate 80mL, dehydroactic acid sodium 600mg, MgSO4
300mg, KNO3150mg, K2HPO4100mg, Triiodobenzoic acid 180mg, EDTA-2Na 10mg, vitamin B110mg, water.
3. manufacture method according to claim 1 and 2 is it is characterised in that described Mother culture condition includes：In 22-26
Constant temperature lucifuge culture at DEG C；And/or,
Described Primary spawn condition includes：Temperature be 20-22 DEG C, humidity be 55-65% under constant temperature lucifuge culture；And/or,
Described cultigen condition of culture includes：Temperature be 20-22 DEG C, humidity be 55-65% under constant temperature culture；In early stage 10 days
Lucifuge is cultivated, and later stage intensity of illumination is 1000-1500 1x；And/or,
Described substituting stuff cultivation condition includes：Cultivation temperature is 28-30 DEG C, and relative air humidity is 60-75%, and early stage was kept away in 10 days
Optical culture, later stage intensity of illumination is 1000-1500 1x.
4. the substrate in a kind of bonsai type container making for live body glossy ganoderma dish garden is it is characterised in that described bonsai type container
In substrate include the raw material of following weight portion：Sandy loam 80-100 part, sawdust 10-30 part, plant ash 1-3 part, Gypsum Fibrosum powder 1-3
Part；Preferably, the substrate in described bonsai type container includes the raw material of following weight portion：85 parts of sandy loam, 12 parts of sawdust, vegetation
2 parts of ash, 2 parts of Gypsum Fibrosum powder.
5. a kind of nutritional solution making for live body glossy ganoderma dish garden is it is characterised in that nutritional solution described in every 1kg includes following one-tenth
Point：Cultivar culture medium leachate 50-100mL, dehydroactic acid sodium 500-1000mg, MgSO4100-500mg, KNO3100-
200mg, K2HPO450-100mg, Triiodobenzoic acid 100-200mg, EDTA-2Na5-10mg, vitamin B15-10mg, water；
Preferably, nutritional solution described in every 1kg includes following component：Cultivar culture medium leachate 80mL, dehydroactic acid sodium
600mg, MgSO4300mg, KNO3150mg, K2HPO4100mg, Triiodobenzoic acid 180mg, EDTA-2Na 10mg, vitamin
B110mg, water.
6. a kind of Cultivar culture medium of cultivating ganoderma or generation material culture medium are it is characterised in that include the raw material of following weight portion：
Wood flour 30-50 part, pig manure 5-15 part, cattle manure 5-15 part, rapeseed meal 5-25 part, humic acidss 5-10 part, Mycen osmundicola fermentation liquid 1-
10 parts, bacillus subtilis fermentation liquor 1-10 part, Gypsum Fibrosum 1-3 part；In described Mycen osmundicola fermentation liquid, living bacteria count is 1.0-
5.0×106/ mL, in described bacillus subtilis fermentation liquor, living bacteria count is 1.0-1.5 × 1010/mL；Described cultigen training
The preparation method of foster base comprises the following steps：By proportioning, each raw material is mixed, add water to water content 50-70%, ferment to becoming thoroughly decomposed；
Adjust moisture to 55-60%, sterilize, you can.
7. Cultivar culture medium according to claim 6 or generation material culture medium are it is characterised in that include following weight portion
Raw material：40 parts of wood flour, 5 parts of pig manure, 15 parts of cattle manure, 15 parts of rapeseed meal, 6 parts of humic acidss, 5 parts of Mycen osmundicola fermentation liquid, hay bud
5 parts of spore bacillus fermentation liquid, 2 parts of Gypsum Fibrosum；In described Mycen osmundicola fermentation liquid, living bacteria count is 1.0-3.0 × 106/ mL, described
In bacillus subtilis fermentation liquor, living bacteria count is 1.0-1.2 × 1010/mL.
8. the Cultivar culture medium according to claim 6 or 7 or generation material culture medium are it is characterised in that described hay spore
Bacillus is CGMCC 1.3358.
9. the Cultivar culture medium according to claim 6 or 7 or generation material culture medium are it is characterised in that described fermentation is to corruption
Ripe inclusion：First time turning when temperature reaches 50 DEG C in fermentation heap, turning daily 1-2 time later, and supplement amount of water, pending
Ferment heap temperature be reduced to less than 35 DEG C, moisture be down to less than 30% end fermentation.
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| CN201910937295.8A CN110521489A (en) | 2016-10-14 | 2016-10-14 | The method for extending living body glossy ganoderma dish garden storage life and improving its preservation quality |
| CN201610900613.XA CN106479903B (en) | 2016-10-14 | 2016-10-14 | A kind of production method of living body glossy ganoderma dish garden |
| CN201910936458.0A CN110476708A (en) | 2016-10-14 | 2016-10-14 | Living body glossy ganoderma dish garden manufacture craft |
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| CN201910937295.8A Division CN110521489A (en) | 2016-10-14 | 2016-10-14 | The method for extending living body glossy ganoderma dish garden storage life and improving its preservation quality |
| CN201910936457.6A Division CN110476707A (en) | 2016-10-14 | 2016-10-14 | Living body glossy ganoderma dish garden |
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| CN110476709A (en) | 2019-11-22 |
| CN110521489A (en) | 2019-12-03 |
| CN106479903B (en) | 2019-10-25 |
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