CN109874676A - A kind of fast breeding method of passion fruit detoxic seedling - Google Patents
A kind of fast breeding method of passion fruit detoxic seedling Download PDFInfo
- Publication number
- CN109874676A CN109874676A CN201910288372.1A CN201910288372A CN109874676A CN 109874676 A CN109874676 A CN 109874676A CN 201910288372 A CN201910288372 A CN 201910288372A CN 109874676 A CN109874676 A CN 109874676A
- Authority
- CN
- China
- Prior art keywords
- detoxic seedling
- seedling
- passion fruit
- culture
- stem section
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to passion fruit technical field of cultivation, disclose a kind of fast breeding method of passion fruit detoxic seedling, parental generation detoxic seedling is obtained by stem apex virus-free culture, parental generation detoxic seedling water planting is carried out again, parental generation stem section tissue virus-free culture obtains filial generation detoxic seedling, filial generation detoxic seedling field-transplanting obtains progeny plant, progeny plant stem section cultivation acquisition passion fruit detoxic seedling.The present invention carries out stem apex using tissue culture technique or stem section culture, nursery speed are fast；And detoxification treatment is carried out in reproductive process, it is possible to reduce viral harm guarantees that passion fruit seedling is best in quality.
Description
Technical field
The present invention relates to passion fruit technical field of cultivation, and in particular to a kind of fast breeding method of passion fruit detoxic seedling.
Background technique
Passion fruit can not only manufacture high-grade fruit drink, be also used as high-grade food additives.Meanwhile passion fruit
Comprehensive development and utilization is also very valuable, and development potentiality is huge, can develop the green product of many new high nutritions, Gao Baojian,
Not only it can eat raw, but also a variety of deep processings can be carried out.Juice can be processed into fruit juice, inspissated juice, jam, jelly, fruit vinegar, fruit
Tea, fruit wine etc..Using the Fragrant fruit wine that passion fruit is produced as raw material, in beautiful color, smell is aromatic, and mouthfeel is first-class, and more drinks are not liquor-saturated,
In good taste, nutritive effect is all peculiar, sutable for men, women, and children, wins warm praise from customers；Passiflora edulis vinegar is even more color beauty, taste perfume, taste
It blurts out in road；With eliminating inflammation and expelling toxin, the special efficacys such as blood fat-reducing blood pressure-decreasing, digestion-promoting silt, skin-protecting face nursing, reducing weight and beautifying features.The nutrition ratio that fruit seed contains
Sunflower seed are more preferable and a kind of good beauty food, can extract vegetable oil or be processed into fruit seed sauce.Passion fruit branches and leaves and fruit
Skin is full of nutrition, can be processed into rare Chinese medicine and high-grade Feed Outlet.
Main problem existing for passion fruit growing industry is exactly the cultivation of seedling, and traditional passion fruit method for culturing seedlings is divided into two
Kind, first is that passion fruit stem section cuttage root-taking is seedling, another kind is to sow to be transferred after obtaining seedling with passion fruit seed
It connects, above-mentioned conventional method sapling multiplication low efficiency, and usually will appear plant virus infection, after many years vegetative propagation, plant
A large amount of accumulation viruses in vivo, cause serious variety deterioration.
Summary of the invention
Based on problem above, the present invention provides a kind of fast breeding method of passion fruit detoxic seedling, utilizes tissue cultures skill
Art carries out stem apex or stem section culture, nursery speed are fast；And detoxification treatment is carried out in reproductive process, it is possible to reduce viral harm,
Guarantee that passion fruit seedling is best in quality.
In order to solve the above technical problems, the present invention provides a kind of fast breeding method of passion fruit detoxic seedling, features
It is, includes the following steps:
S1, acquisition 8~10cm robust growth and have stem apex passion fruit stem section tissue, with mass fraction be 0.2% it is more
With culture solution culture 21 days under 35 DEG C of gnotobasis after 20~30min of bacterium spirit solution immersion；
S2, stem apex part in step S1 is cut, length is 2~3cm, aseptically by stem apex with 70~75%
Alcohol infiltrates 25~30 seconds, then the carbendazim solution for being 0.2% with mass fraction impregnates 20~30min, is then rushed with sterile water
It washes 3~4 times；
Under S3, gnotobasis by stem apex after step S2 processing on the 1/2MS culture medium that concentration is 0.1mg/LNAA
Tissue cultures are carried out, induces it to take root, obtains parental generation detoxic seedling；Plant is taken out when parental generation detoxic seedling 2~3cm of root long, is cleaned
Its root is simultaneously transplanted in culture solution and carries out water planting；When water planting intensity of illumination be 2500~3500Lux, light application time be 10.0~
Under conditions of 12.0h, temperature are 23~27 DEG C, cultivate 25~30 days；
S4, acquire parental generation detoxic seedling 8~10cm stem section, with mass fraction be 0.2% carbendazim solution impregnate 20~
30 minutes, then stem section is cultivated in the 1/2MS culture medium of 0.1mg/LNAA；After stem section is taken root, it is de- to obtain filial generation
Malicious seedling；
S5, by resulting bottle for detoxification transplantation of seedlings crop field, carry out field management after its Adaptable growth, obtain progeny plant；
S6, the healthy and strong stem section acquired from progeny plant, in an aseptic environment with carried out on aerosol seedling rearing room cultivate take root
Afterwards to get arrive finished market product seedling.
Further, the ingredient of culture solution is calcium nitrate 1250mg/L, potassium nitrate 250mg/L, phosphoric acid in step S1 and S3
Ammonium dihydrogen 350mg/L, magnesium sulfate 540mg/L, ammonium nitrate 240mg/L, sodium iron ethylene diamine tetra acetate 40mg/L, ferrous sulfate
15mg/L, boric acid 2.86mg/L, manganese sulfate 2.13mg/L, copper sulphate 0.05mg/L, zinc sulfate 0.22mg/L, ammonium molybdate
0.02mg/L。
Further, it is 5~10% that mass fraction, which is added, in the 1/2MS culture medium of 0.1mg/LNAA in step S3 and S4
The caseinhydrolysate of antibiotic, the gibberellin of 0.5~1.0mg/L and 100~200mg/L is cultivated.
Further, antibiotic is penicillin or streptomysin
Further, after stem section is taken root in step S4, every 5 days in potassium dihydrogen phosphate of foliage spray, to root
It is to stop sprinkling after length reaches 10cm.
Compared with prior art, the beneficial effects of the present invention are: the present invention utilizes tissue culture technique, pass through stem apex detoxification
Culture obtain parental generation detoxic seedling, then carry out parental generation detoxic seedling water planting, parental generation stem section tissue virus-free culture obtains filial generation detoxic seedling, son
Progeny plant is obtained for detoxic seedling field-transplanting, progeny plant stem section, which is cultivated, obtains passion fruit detoxic seedling, and nursery speed is fast；And it is numerous
Detoxification treatment is carried out during educating, it is possible to reduce viral harm guarantees that passion fruit seedling is best in quality.
Detailed description of the invention
Fig. 1 is the fast breeding method flow chart of passion fruit detoxic seedling in embodiment 1；
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below with reference to embodiment and attached drawing, to this
Invention is described in further detail, and exemplary embodiment of the invention and its explanation for explaining only the invention, are not made
For limitation of the invention.
Embodiment 1:
Referring to Fig. 1, a kind of fast breeding method of passion fruit detoxic seedling, which comprises the steps of:
S1, acquisition 8~10cm robust growth and have stem apex passion fruit stem section tissue, with mass fraction be 0.2% it is more
With culture solution culture 21 days under 35 DEG C of gnotobasis after 20~30min of bacterium spirit solution immersion；Passion fruit stem section is pre-processed,
It is woven in passion fruit stem section group in culture solution and fully absorbs nutriment, robust growth.
S2, stem apex part in step S1 is cut, length is 2~3cm, aseptically by stem apex with 70~75%
Alcohol infiltrates 25~30 seconds, then the carbendazim solution for being 0.2% with mass fraction impregnates 20~30min, is then rushed with sterile water
It washes 3~4 times, realizes the detoxification of passion fruit stem apex.
Under S3, gnotobasis by stem apex after step S2 processing on the 1/2MS culture medium that concentration is 0.1mg/LNAA
Tissue cultures are carried out, induces it to take root, obtains parental generation detoxic seedling；Plant is taken out when parental generation detoxic seedling 2~3cm of root long, is cleaned
Its root is simultaneously transplanted in culture solution and carries out water planting；When water planting intensity of illumination be 2500~3500Lux, light application time be 10.0~
Under conditions of 12.0h, temperature are 23~27 DEG C, cultivate 25~30 days；
S4, acquire parental generation detoxic seedling 8~10cm stem section, with mass fraction be 0.2% carbendazim solution impregnate 20~
30 minutes, then stem section is cultivated in the 1/2MS culture medium of 0.1mg/LNAA；After stem section is taken root, it is de- to obtain filial generation
Malicious seedling；
S5, by resulting bottle for detoxification transplantation of seedlings crop field, carry out field management after its Adaptable growth, obtain progeny plant；
S6, the healthy and strong stem section acquired from progeny plant, in an aseptic environment with carried out on aerosol seedling rearing room cultivate take root
Afterwards to get arrive finished market product seedling.
In the present embodiment, aerosol seedling rearing room includes cultivating chamber, and cultivation bed is vacantly arranged in cultivating chamber；It needs to carry out aerosol to educate
Plant stem section or root system are inserted on cultivation bed by Miao Shi, and root system or stem section notch are exposed inside cultivating chamber, utilize spraying device
It is small droplet shape by nutrient liquid atomizing, is directly injected to root system of plant or stem section with moisture needed for providing plant growth and supports
Point.The contradiction that insoluble moisture, air, nutrient supply in conventional soil cultivation can effectively be solved, is in crop root
Under optimum environmental condition, to play the growth potential of crop, plant growth amount, biomass is made to be greatly enhanced.
The present invention utilizes tissue culture technique, obtains parental generation detoxic seedling by stem apex virus-free culture, then carry out parental generation detoxification
Seedling water planting, parental generation stem section tissue virus-free culture obtain filial generation detoxic seedling, filial generation detoxic seedling field-transplanting obtains progeny plant, filial generation
Plant stem section, which is cultivated, obtains passion fruit detoxic seedling, and nursery speed is fast；And detoxification treatment is carried out in reproductive process, it is possible to reduce virus
Harm, guarantee passion fruit seedling it is best in quality.
Embodiment 2:
S1, acquisition 8~10cm robust growth and have stem apex passion fruit stem section tissue, with mass fraction be 0.2% it is more
With culture solution culture 21 days under 35 DEG C of gnotobasis after bacterium spirit solution immersion 30min.The ingredient of culture solution is calcium nitrate
1250mg/L, potassium nitrate 250mg/L, ammonium dihydrogen phosphate 350mg/L, magnesium sulfate 540mg/L, ammonium nitrate 240mg/L, ethylenediamine tetraacetic
Acetic acid ferrisodium 40mg/L, ferrous sulfate 15mg/L, boric acid 2.86mg/L, manganese sulfate 2.13mg/L, copper sulphate 0.05mg/L, sulfuric acid
Zinc 0.22mg/L, ammonium molybdate 0.02mg/L
S2, cut stem apex part in step S1, length is 2~3cm, aseptically by stem apex with 75% alcohol
Infiltration 30 seconds, then the carbendazim solution for being 0.2% with mass fraction impregnate 30min, then use aseptic water washing 3~4 times；
Under S3, gnotobasis by stem apex after step S2 processing on the 1/2MS culture medium that concentration is 0.1mg/LNAA
Tissue cultures are carried out, induces it to take root, obtains parental generation detoxic seedling；It is 5~10% that mass fraction is added before culture in the medium
Penicillin, the gibberellin of 0.5~1.0mg/L and the caseinhydrolysate of 100~200mg/L.To parental generation detoxic seedling root long 2~
Plant is taken out when 3cm, is cleaned its root and is transplanted to progress water planting in culture solution and (cultivates in water planting culture solution ingredient and step S1
Liquid ingredient is identical)；Under conditions of intensity of illumination is 3000Lux when water planting, light application time 12.0h, temperature are 25 DEG C, culture 30
It；
S4, acquire parental generation detoxic seedling 8~10cm stem section, with mass fraction be 0.2% carbendazim solution impregnate 20~
30 minutes, then stem section is cultivated in the 1/2MS culture medium of 0.1mg/LNAA, quality is added in the medium before culture
Penicillin, the gibberellin of 0.5~1.0mg/L and the caseinhydrolysate of 100~200mg/L that score is 5~10%.It is raw to stem section
After root, every 5 days in potassium dihydrogen phosphate of foliage spray, stops sprinkling after root length reaches 10cm, obtain son
For detoxic seedling；
S5, by resulting bottle for detoxification transplantation of seedlings crop field, carry out field management after its Adaptable growth, obtain progeny plant；
S6, the healthy and strong stem section acquired from progeny plant, in an aseptic environment with carried out on aerosol seedling rearing room cultivate take root
Afterwards to get arrive finished market product seedling.
The antibiotic used in the present embodiment can have suppression to microorganisms such as bacteriums in tissue culture procedures for penicillin
Production is used.It in addition to penicillin, can also play the role of that the microorganisms such as bacterium is inhibited to grow using streptomysin.
It as above is the embodiment of the present invention.Design parameter in above-described embodiment and embodiment is merely to understand table
Invention verification process is stated, the scope of patent protection being not intended to limit the invention, scope of patent protection of the invention is still with it
It is all to change with equivalent structure made by specification and accompanying drawing content of the invention subject to claims, it should all similarly wrap
Containing within the scope of the present invention.
Claims (5)
1. a kind of fast breeding method of passion fruit detoxic seedling, which comprises the steps of:
S1, pretreatment: acquiring 8~10cm robust growth and has stem apex passion fruit stem section tissue, is 0.2% with mass fraction
With culture solution culture 21 days under 35 DEG C of gnotobasis after 20~30min of carbendazim solution immersion；
S2, stem apex detoxification: cutting the stem apex part in step S1, and length is 2~3cm, aseptically by stem apex with 70~
75% alcohol infiltrates 25~30 seconds, then the carbendazim solution for being 0.2% with mass fraction impregnates 20~30min, then uses nothing
Bacterium water rinses 3~4 times；
S3, parental generation detoxic seedling culture: by stem apex after step S2 processing in 1/ that concentration is 0.1mg/LNAA under gnotobasis
Tissue cultures are carried out on 2MS culture medium, it is induced to take root, and obtain parental generation detoxic seedling；It is taken when parental generation detoxic seedling 2~3cm of root long
Plant out, cleans its root and is transplanted in culture solution and carry out water planting；Intensity of illumination is 2500~3500Lux, illumination when water planting
Under conditions of time is 10.0~12.0h, temperature is 23~27 DEG C, cultivate 25~30 days；
S4, filial generation detoxic seedling culture: 8~10cm stem section of acquisition parental generation detoxic seedling, the carbendazim for being 0.2% with mass fraction are molten
Liquid impregnates 20~30 minutes, then cultivates stem section in the 1/2MS culture medium of 0.1mg/LNAA；After stem section is taken root,
Obtain filial generation detoxic seedling；
S5, transplanting crop field: by resulting bottle for detoxification transplantation of seedlings crop field, field management is carried out after its Adaptable growth, obtains filial generation
Plant；
S6, commercial seedling culture: the healthy and strong stem section acquired from progeny plant is trained on aerosol seedling rearing room in an aseptic environment
It educates after taking root to get to finished market product seedling.
2. the fast breeding method of passion fruit detoxic seedling according to claim 1, which is characterized in that trained in step S1 and S3
The ingredient of nutrient solution is calcium nitrate 1250mg/L, potassium nitrate 250mg/L, ammonium dihydrogen phosphate 350mg/L, magnesium sulfate 540mg/L, nitric acid
Ammonium 240mg/L, sodium iron ethylene diamine tetra acetate 40mg/L, ferrous sulfate 15mg/L, boric acid 2.86mg/L, manganese sulfate 2.13mg/L,
Copper sulphate 0.05mg/L, zinc sulfate 0.22mg/L, ammonium molybdate 0.02mg/L.
3. the fast breeding method of passion fruit detoxic seedling according to claim 1, which is characterized in that in step S3 and S4
The 1/2MS culture medium of 0.1mg/LNAA be added mass fraction be 5~10% antibiotic, 0.5~1.0mg/L gibberellin and
The caseinhydrolysate of 100~200mg/L is cultivated.
4. the fast breeding method of passion fruit detoxic seedling according to claim 3, which is characterized in that the antibiotic is blueness
Mycin or streptomysin.
5. the fast breeding method of passion fruit detoxic seedling according to claim 1, which is characterized in that stem section is raw in step S4
After root, every 5 days in potassium dihydrogen phosphate of foliage spray, stop sprinkling after root length reaches 10cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910288372.1A CN109874676A (en) | 2019-04-11 | 2019-04-11 | A kind of fast breeding method of passion fruit detoxic seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910288372.1A CN109874676A (en) | 2019-04-11 | 2019-04-11 | A kind of fast breeding method of passion fruit detoxic seedling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109874676A true CN109874676A (en) | 2019-06-14 |
Family
ID=66936894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910288372.1A Pending CN109874676A (en) | 2019-04-11 | 2019-04-11 | A kind of fast breeding method of passion fruit detoxic seedling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109874676A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771730A (en) * | 2020-08-31 | 2020-10-16 | 广东省湛江农垦科学研究所 | Production method of passion fruit virus-free seedlings |
| CN117084171A (en) * | 2023-09-05 | 2023-11-21 | 广西壮族自治区中国科学院广西植物研究所 | Detoxification method of passion fruits |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340744A (en) * | 2015-11-17 | 2016-02-24 | 浦北南国水果种植农民专业合作社 | Passion fruit tissue culture seedling method |
| CN105706935A (en) * | 2016-03-14 | 2016-06-29 | 龙岩市禾康生物科技有限公司 | Method for cultivating passion fruit virus-free seedlings |
| CN107896993A (en) * | 2017-12-11 | 2018-04-13 | 江可以 | The culture technique of passion fruit detoxic seedling |
| CN108432632A (en) * | 2018-02-10 | 2018-08-24 | 广西亿绿千城农业科技有限公司 | A kind of fast breeding method of passion fruit detoxic seedling |
-
2019
- 2019-04-11 CN CN201910288372.1A patent/CN109874676A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340744A (en) * | 2015-11-17 | 2016-02-24 | 浦北南国水果种植农民专业合作社 | Passion fruit tissue culture seedling method |
| CN105706935A (en) * | 2016-03-14 | 2016-06-29 | 龙岩市禾康生物科技有限公司 | Method for cultivating passion fruit virus-free seedlings |
| CN107896993A (en) * | 2017-12-11 | 2018-04-13 | 江可以 | The culture technique of passion fruit detoxic seedling |
| CN108432632A (en) * | 2018-02-10 | 2018-08-24 | 广西亿绿千城农业科技有限公司 | A kind of fast breeding method of passion fruit detoxic seedling |
Non-Patent Citations (1)
| Title |
|---|
| MUKASA ET AL.: "IN VITRO PROPAGATION OF THREE COMMERCIAL PASSIONFRUIT VARIETIES IN UGANDA", 《AFRICAN CROP SCIENCE JOURNAL》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771730A (en) * | 2020-08-31 | 2020-10-16 | 广东省湛江农垦科学研究所 | Production method of passion fruit virus-free seedlings |
| CN117084171A (en) * | 2023-09-05 | 2023-11-21 | 广西壮族自治区中国科学院广西植物研究所 | Detoxification method of passion fruits |
| CN117084171B (en) * | 2023-09-05 | 2024-05-03 | 广西壮族自治区中国科学院广西植物研究所 | Detoxification method of passion fruits |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104145816B (en) | Bletilla striata tissue culture method | |
| CN103416305B (en) | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN102499088B (en) | Method for quickly breeding seedlings of Guangxi anoectochilus roxburghii capsules by utilizing Guangxi anoectochilus roxburghii capsules | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN103270952B (en) | Sinkiang drug mulberry tissue culture and rapid propagation culture medium | |
| CN109258460A (en) | Micro-stem tip culture combines the breeding method of heat treatment acquisition Zengcheng honey chrysanthemum detoxic seedling | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| CN103704130A (en) | Chinese orchid and cymbidium hybridum hybrid seedling raising method | |
| CN105850502A (en) | Plateau morchella planting method | |
| CN109220790B (en) | In vitro propagation method of rhododendron simsii | |
| CN110476707A (en) | Living body glossy ganoderma dish garden | |
| CN101695280B (en) | Tissue culture and rapid propagation method of raspberries | |
| CN109874676A (en) | A kind of fast breeding method of passion fruit detoxic seedling | |
| CN110214702A (en) | Dendrobidium huoshanness tissue-cultured seedling is cultivated and hardening off method | |
| CN103250564B (en) | Artificial cultivating method for chestnut mycorrhiza fungi | |
| CN105706872A (en) | Bletilla striata seed direct seeding natural reproduction seedling method | |
| CN105340708A (en) | Industrialized seedling production method of panax notoginseng | |
| CN105875412B (en) | The implantation methods of organic selenium-rich sugariness gynostemma pentaphylla | |
| CN103782912A (en) | Culture medium for red cassia tree tissue culture | |
| CN113331054B (en) | Plant tissue culture method by adding culture medium under bacteria environment condition | |
| CN105028196A (en) | Quick tissue culture seedling breeding method of decaisne fargesii | |
| CN115024221A (en) | Method for rapidly propagating tissue culture seedlings of large-leaf morinda officinalis and application of method | |
| CN106258966A (en) | A kind of method of cultivating nursery of Passifolra edulis | |
| CN106417012B (en) | A kind of tissue culture method for promoting bamboo shoot orchid efficiently to breed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190614 |
|
| RJ01 | Rejection of invention patent application after publication |