CN106258966A - A kind of method of cultivating nursery of Passifolra edulis - Google Patents
A kind of method of cultivating nursery of Passifolra edulis Download PDFInfo
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- CN106258966A CN106258966A CN201610660558.1A CN201610660558A CN106258966A CN 106258966 A CN106258966 A CN 106258966A CN 201610660558 A CN201610660558 A CN 201610660558A CN 106258966 A CN106258966 A CN 106258966A
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- China
- Prior art keywords
- passifolra edulis
- propagation
- fruit
- carry out
- edulis
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The present invention is applicable to cultivation of plants technological improvement field, it is provided that a kind of method cultivating nursery of Passifolra edulis, said method comprising the steps of: A, configuration Nutrient medium；B, shoot to Passifolra edulis carry out strain body and carry out disinfection；C, root induction are cultivated；D, survival after transplant.Can provide production batches of high quality seedling by detoxification isolated culture and Fast-propagation industrial seedling rearing, it is to avoid owing to cottage propagation causes spreading of disease；It addition, by tissue culture rapid propagation technique, Passifolra edulis hybrid vigor can be kept, it is to avoid owing to seminal propagation causes hybrid separation.
Description
Technical field
The invention belongs to, in cultivation of plants technical field, particularly relate to a kind of method cultivating nursery of Passifolra edulis.
Background technology
Passifolra edulis formal name used at school Herba Passiflorae Caeruleae, has another name called Passiflora edulis sims, originates in South America, belongs to the Passifolra edulis section torrid zone, subtropical zone perennial often
Green liana, its fruit is the typical torrid zone, subtropical zone berry.Fruit juice fragrance is unique, and delicious flavour becomes rich in various nutrition
Point, it is preferable high-grade drink, and root, stem, leaf, flower also can be used as medicine.In recent years, international market to the demand of Passifolra edulis fruit juice with
The speed increment of annual 15%～20%, has greatly the gesture that supply falls short of demand.
Passifolra edulis contains 17 kinds of aminoacid, rich in protein, fat, sugar, vitamin, calcium, phosphorus, ferrum, potassium, SOD enzyme
With superfluorescent fiber etc. 165 kinds to human body benefit materials, the king of the VC being more referred to as in fruit, mouthfeel is unique.Passifolra edulis has antiinflammatory
Pain relieving, invigorate blood circulation keep fit, blood fat-reducing blood pressure-decreasing, enriching yin and nourishing kidney, relieving alcoholic intoxication of refreshing oneself, allaying tiredness, the magical effect such as skin-protecting face nursing, be fabricated to drink
Material, cold and hot drink is all suitable, excellent in taste, and normal drink also eliminates comedo, eliminates hemorrhoid, elimination gastroenteritis.Lumbricus contains abundant
Aminoacid, enzyme, vitamin and active substance, the most traditional medical material, may be used to develop plurality kinds of health care food, cosmetic
Product；Or the important sources of the animal protein of poultry farming, freshwater fish culturing feedstuff.Especially because Lumbricus can be with work
Industry, agricultural and exhausting organic waste are food, and are translated into the antibiotic property organic fertilizer Lumbricus feces of high-quality, therefore
For environmental protection and Development of Organic Agriculture, there is important economic implications and realistic meaning, process house refuse and straw stalk with Lumbricus
The like waste, reduces and burns the smog produced, and is also the effective measure of development low-carbon economy.Along with the quickening of urbanization process,
Agricultural cultivating & ground area reduces year by year, and land resource is the most in short supply, how can overlap on soil and to support interplanting, to improve the use in soil
Rate and economic benefit, be also an important topic anxious to be resolved.
Passifolra edulis, has another name called Herba Passiflorae Caeruleae, because its sarcocarp can give out Fructus Musae, Fructus Ananadis comosi, Fructus Fragariae Ananssae, Fructus Litchi, Fructus Citri Limoniae, Fructus Mangifera Indicae, dark plum
Being described as " Passifolra edulis " Deng the strong fragrance of ten several fruit, Passifolra edulis is rich in 17 kinds of aminoacid, multiple of needed by human body
Vitamin and carotenoid and various trace element, the most nutritious, and delicious flavour.Passifolra edulis adaptability is extensive,
In the south of China, equal suitable planting throughout the year, in the north, winter suitably uses vegetables greenhouse cultivation.
Passifolra edulis, is commonly called as " brazilnut ", " Passiflora edulis sims ", originates in Brazil, belongs to Passifloraceae.Because its fruit juice nutrient enriches,
Abnormal smells from the patient is the most fragrant, can give out Fructus Musae, Fructus Ananadis comosi, Fructus Citri Limoniae, Fructus Fragariae Ananssae, the strong fragrance of kind various fruits such as Fructus Persicae, Punica granatum L. and lifted
For " Passifolra edulis ", there is again the laudatory title of " Passifolra edulis " " king of fruit juice ", because its fruit juice is also its English name " Passionfruit "
Transliteration.Passifolra edulis, the using names because of the fragrance containing numerous fruits, formal name used at school Herba Passiflorae Caeruleae, it is commonly called as " brazilnut ") also the hottest in Hong Kong
Feelings fruit) its English entitled " Passion Fruit ", " Passion " means " enthusiasm, love ", therefore " the love that is otherwise known as
Really ", " sweet heart fruit ", original producton location is in South America.Its fruit juice is rich in the multiple element useful to human body.Passifolra edulis contains the abundantest
Natural vitamin C, just containing vitamin C in every 100 grams of fruit juice is 34.6 milligrams, also rich in Vitamin A, B1, B2 etc.,
Additionally possibly together with materials such as abundant calcium, phosphorus, ferrum and several amino acids.
Containing 165 kinds of fruit aromas such as Fructus Ananadis comosi, Fructus Musae, Fructus Fragariae Ananssae, Fructus Mali pumilae, dark plum, Fructus Mangifera Indicae in Passifolra edulis, its fruit contains
Having more than the aromatic substance of more than 132 kinds, fruit juice nutrient enriches, and fragrant odour is spice fruit world-famous, has " fruit juice
King " good reputation；Passifolra edulis also has the refined title of " enthusiasm fruit ", and it is that a kind of perennial torrid zone liana seeks connections with fruit tree, sweet fruit
Acid is good to eat, with rich flavor, and fragrance is pleasant；It originates from the Amazon River Tropical rain forest of South America, by Hispanic explorer, repaiies
" Synsepalum duleificum " that the mankind first ancestor " Adam, Eve " that Taoist priest is considered in " Holy Bible " to mention is eaten, therefore English is referred to as
" PassionFruit ", means " love, passion, enthusiasm fruit ".
Passifolra edulis belongs to diclinism, female flower result, and male flower is used for pollinating, and its pollination medium is mainly Apis and insecticide,
Also artificial pollination can be passed through.The Passifolra edulis of plantation in spring, can bloom then July, and JIUYUE can receive fruit.After Second Year, every year
Starting April to bloom, within 1 year, can open 5-6 and criticize, every batch of flower is separated by 20 days, needs 60-70 days from blooming to fruit maturation, and fruit is received
Obtain 4 wheat harvesting periods.
The propagation method of Passifolra edulis mainly has two kinds, and one is with the direct seed propagation of seed, and another kind is then to use climing bar
Cottage propagation.Every mu of plantation 70-80 strain master is climing, and the main climing lateral bine that separates step by step is climbed on frame and prolonged result.Annual every mu of Passifolra edulis
Yield reaches 1-1.5 ton, high yield reach more than 2 tons.
Passifolra edulis originates in South America and belongs to perennial evergreen climber liana tropical fruit (tree), because its fruit juice has Fructus psidii guajavae immaturus, awns
Really, the fragrance of the various fruits such as Fructus Musae and gain the name.Passifolra edulis strong adaptability, grow vigorous, Resistant, go into operation early, yield is high, product
Matter is good, and storage tolerance, market are wide, and high financial profit has the laudatory title of " money tree " abroad.Passifolra edulis has the strong of various fruits
Fragrance, and the highest nutritive value, not only can eat as fresh fruit and fruit drink processed, is the most also with processing fruit jam, fruit jelly, ice river in Henan Province
Drench, cake, cookies optimal processing fruit.Passifolra edulis fruit juice as a kind of pure natural " green " fruit drink with its color, smell and taste
All good and good health-care effect, all-ages, righteous style and practice is rubbed the world.
Passifolra edulis belongs to climing footpath property fruit tree, is suitable for warm, annual without the area kind on the south the north latitude 24 degree of Freezing injury weather
Plant.The propagation method of Passifolra edulis mainly has 2 kinds, and one is with the direct seed propagation of seed, and two is with climing bar cottage propagation.By kind
Son directly seed propagation easily causes hybrid separation, there is quality meta-acid, short sweetness, problem that yield is the highest；And it is not added with choosing
Selecting ground arbitrarily cottage propagation, accelerate the degeneration of kind, disease increases, and is particularly easy to infect stem rot, and yield is substantially reduced.
Summary of the invention
It is an object of the invention to provide a kind of method cultivating nursery of Passifolra edulis, it is intended to solve above-mentioned technology and ask
Topic.
The present invention is achieved in that a kind of method cultivating nursery of Passifolra edulis, said method comprising the steps of:
A, configuration Nutrient medium；
B, shoot to Passifolra edulis carry out strain body and carry out disinfection；
C, root induction are cultivated；
D, survival after transplant.
The further technical scheme of the present invention is: configuration Nutrient medium adds the sucrose and 0.8%～1.1% of 4%～7%
Agar, with NaOH solution adjust pH to 6.0～6.5 obtain minimal medium, minimal medium is dispensed into 250mL's
In culture bottle, standby after sterilizing 25min under 118～121 DEG C, 0.12～0.16MPa pressure.
The further technical scheme of the present invention is: the sterilization of strain body uses ethanol wash 35S.
The invention has the beneficial effects as follows: production can be provided in batch by detoxification isolated culture and Fast-propagation industrial seedling rearing
High quality seedling, it is to avoid owing to cottage propagation causes spreading of disease；It addition, by tissue culture rapid propagation technique, can protect
Hold Passifolra edulis hybrid vigor, it is to avoid owing to seminal propagation causes hybrid separation.
Detailed description of the invention
The method cultivating nursery of a kind of Passifolra edulis that the present invention provides, details are as follows for it:
Step A, configures Nutrient medium；Weigh 9g agar, 30g sucrose, put in the enamel graduate of 1000mL, add distillation
Water 750mL, with heating by electric cooker, heating edge Glass rod in limit stirs, the shape until liquid is translucent；The most again by MS culture medium
Join in the agar boiled, finally add distilled water and be settled to 1000mL, stir, with the NaOH solution of 1mol/L, by
Instill in the culture medium dissolved, drip while stir, adjust pH to 5.8, obtain minimal medium；Minimal medium is dispensed into
In the culture bottle of 200mL, at 120 DEG C, sterilizing 20min under 0.1MPa pressure, standby.
Step B, carries out strain body to the shoot of Passifolra edulis and carries out disinfection；Anosis in stalwartness in noon or the afternoon of fair weather
The stem section that on the plant of evil, clip 5cm segment children is tender, each
Stem section contains 2 joints, removes the blade on cardinal extremity joint, stays 1 leaf of joint, soaks 3min in the liquid detergent stirred evenly,
Rinse 1h with tap water flowing water again, aseptically, with the ethanol wash 30s of 75%, re-use the mercuric chloride solution of 0.1%
Sterilizing 10min, with aseptic water washing 3 times, standby.
Step C, root induction is cultivated；When adventitious bud length to 2cm height, the seedling of shape of growing thickly is cut into individual plant seedling,
Access in minimal medium, the naphthalene acetic acid of additional 0.2mg/L and the indolebutyric acid of 0.1mg/L, carry out root induction.
Step D, survival after transplant.When plant root length to 3cm, culture bottle is moved into greenhouse from culturing room, opens lid,
Exercise 2～3d under natural light；Seedling through exercise is taken out with tweezers, puts into and washes away the training being attached to root in clear water
Support base, plant in the nutritive cube of band Nutrition Soil；After planting, water foot and determine root water, be positioned in the hot house with sunshade net,
Note shading, moisturizing, ventilation；Just can grow make new advances root and young leaves after 10～15d, be transplanted to orchard, routinely Cultivate administration,
Transplanting survival rate is up to more than 95%.
Can provide production batches of high quality seedling by detoxification isolated culture and Fast-propagation industrial seedling rearing, it is to avoid due to
Cottage propagation causes spreading of disease；It addition, by tissue culture rapid propagation technique, Passifolra edulis hybrid vigor can be kept, keeps away
Exempt from owing to seminal propagation causes hybrid separation.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any amendment, equivalent and the improvement etc. made within god and principle, should be included within the scope of the present invention.
Claims (3)
1. the method cultivating nursery of a Passifolra edulis, it is characterised in that said method comprising the steps of:
A, configuration Nutrient medium；
B, shoot to Passifolra edulis carry out strain body and carry out disinfection；
C, root induction are cultivated；
D, survival after transplant.
Method the most according to claim 1, it is characterised in that configuration Nutrient medium adds the sucrose and 0.8% of 4%～7%
～the agar of 1.1%, adjust pH to 6.0～6.5 to obtain minimal medium with NaOH solution, minimal medium is dispensed into
In the culture bottle of 250mL, standby after sterilizing 25min under 118～121 DEG C, 0.12～0.16MPa pressure.
Method the most according to claim 2, it is characterised in that the sterilization of strain body uses ethanol wash 35S.
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| CN201610660558.1A CN106258966A (en) | 2016-08-12 | 2016-08-12 | A kind of method of cultivating nursery of Passifolra edulis |
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| CN201610660558.1A CN106258966A (en) | 2016-08-12 | 2016-08-12 | A kind of method of cultivating nursery of Passifolra edulis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073977A (en) * | 2019-05-15 | 2019-08-02 | 福建农林大学 | Gold passion fruit blade induces differentiation into seedling-growing method |
| CN114938807A (en) * | 2022-05-06 | 2022-08-26 | 中国热带农业科学院海口实验站 | Method for preparing chromosome specimen of root tip meristematic region of passionfruit subgenus plant |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340744A (en) * | 2015-11-17 | 2016-02-24 | 浦北南国水果种植农民专业合作社 | Passion fruit tissue culture seedling method |
| CN105706935A (en) * | 2016-03-14 | 2016-06-29 | 龙岩市禾康生物科技有限公司 | Method for cultivating passion fruit virus-free seedlings |
-
2016
- 2016-08-12 CN CN201610660558.1A patent/CN106258966A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340744A (en) * | 2015-11-17 | 2016-02-24 | 浦北南国水果种植农民专业合作社 | Passion fruit tissue culture seedling method |
| CN105706935A (en) * | 2016-03-14 | 2016-06-29 | 龙岩市禾康生物科技有限公司 | Method for cultivating passion fruit virus-free seedlings |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073977A (en) * | 2019-05-15 | 2019-08-02 | 福建农林大学 | Gold passion fruit blade induces differentiation into seedling-growing method |
| CN114938807A (en) * | 2022-05-06 | 2022-08-26 | 中国热带农业科学院海口实验站 | Method for preparing chromosome specimen of root tip meristematic region of passionfruit subgenus plant |
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Application publication date: 20170104 |
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