CN107896993A - The culture technique of passion fruit detoxic seedling - Google Patents
The culture technique of passion fruit detoxic seedling Download PDFInfo
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- CN107896993A CN107896993A CN201711310783.3A CN201711310783A CN107896993A CN 107896993 A CN107896993 A CN 107896993A CN 201711310783 A CN201711310783 A CN 201711310783A CN 107896993 A CN107896993 A CN 107896993A
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- passion fruit
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The present invention is suitable for plant toxic seedling culture technique field, there is provided a kind of cultural method of passion fruit detoxic seedling, including：A, plant explant pre-treatment；B, basis culture；C, the disinfection of explant；D, virus-free culture；E, tissue-culturing rapid propagation culture is bred；F, culture of rootage.It is easy to operate, industrialized production available for large batch of passion fruit detoxic seedling, after detoxic seedling plantation at the same time, effectively reduce the disease rate of passion fruit plant, compared with passion fruit seed of the routine without detoxification, incidence is only 2% after the passion fruit detoxic seedling plantation of the present invention, substantially increases the economic benefit of passion fruit.
Description
Technical field
The invention belongs to plant toxic seedling culture technique field, more particularly to a kind of cultural method of passion fruit detoxic seedling.
Background technology
Passion fruit contains 17 kinds of amino acid, abundant protein, fat, sugar, vitamin, calcium, phosphorus, iron, potassium, SOD enzymes
With 165 kinds of superfluorescent fiber etc. to human body benefit materials, more it is referred to as the king of the VC in fruit, it is unique in taste.Passion fruit has anti-inflammatory
Analgesic, promoting blood circulation, blood fat-reducing blood pressure-decreasing, enriching yin and nourishing kidney, refresh oneself sober up, the magical effect such as dispelling fatigue, skin-protecting face nursing, be fabricated to drink
Material, all suitable, the excellent in taste of cold and hot drink, often drink, which also has, eliminates whelk, eliminates hemorrhoid, eliminates enterogastritis.Earthworm contains abundant
Amino acid, enzyme, vitamin and active material, still traditional medicinal material, not can be used to develop plurality kinds of health care food, makeup
Product；Or the important sources of the animal protein of poultry farming, freshwater fish culturing feed.Especially because earthworm can be with work
Industry, agricultural and exhausting organic waste are food, and are translated into good antibiotic property organic fertilizer earthworm manure, therefore
There is important economic implications and realistic meaning for environmental protection and Development of Organic Agriculture, house refuse and straw are handled with earthworm
The like waste, reduces the smog for burning and producing, and the effective measure of development low-carbon economy.With the quickening of urbanization process,
Agricultural cultivated area is reduced year by year, and land resource is increasingly in short supply, how on soil intercropping interplant, to improve the use in soil
Rate and economic benefit, and an important topic anxious to be resolved.
Passion fruit, also known as passionflower, because its pulp can give out banana, pineapple, strawberry, lichee, lemon, mango, smoked plum
" passion fruit " is known as Deng the strong fragrance of ten several fruit, ten seven kind amino acid, a variety of of the passion fruit rich in needed by human body
Vitamin and carotenoid and various trace elements, it is not only full of nutrition, but also delicious flavour.Passion fruit adaptability
Extensively, in the south of China, equal suitable planting throughout the year, in the north, winter suitably uses vegetables greenhouse cultivation.
Passion fruit, is commonly called as " Brazil nut ", " passion fruit ", originates in Brazil, belongs to Passifloraceae.Because its fruit juice nutrient enriches,
Smell is especially fragrant, can give out the strong fragrances of various fruits such as banana, pineapple, lemon, strawberry, kind peach, pomegranate and be lifted
For " passion fruit ", and there is the laudatory title of " passion fruit " " king of fruit juice ", because its fruit juice is also its English name " Passionfruit "
Transliteration.Passion fruit, the using names because of the fragrance containing numerous fruits, scientific name passionflower, is commonly called as " Brazil nut ") also heat is also referred to as in Hong Kong
Feelings fruit) entitled " Passion Fruit ", " Passion " is meant " enthusiasm, love ", therefore " the love that is otherwise known as it English
Fruit ", " sweet heart fruit ", original producton location is in South America.Its fruit juice is rich in a variety of elements beneficial to human body.Passion fruit contains very rich
Rich natural Vitamin C, is just 34.6 milligrams containing Vitamin C in every 100 grams of fruit juice, also rich in Alphalin, B1, B2
Deng in addition also containing materials such as abundant calcium, phosphorus, iron and a variety of amino acid.
Contain 165 kinds of fruit aromas such as pineapple, banana, strawberry, apple, smoked plum, mango in passion fruit, contain in its fruit
More than 132 kinds of aromatic substance is had more than, fruit juice nutrient enriches, fragrant odour, is spices fruit world-famous, there is " fruit juice
King " good reputation；Passion fruit also has the refined title of " fruit of enthusiasm ", it is that a kind of perennial tropical liana seeks connections with fruit tree, sweet fruit
Acid is tasty, with rich flavor, and fragrance is pleasant；It originates from the Amazon River tropical rain forest of South America, by Hispanic explorer, repaiies
Taoist priest is considered " miracle fruit " that the mankind first ancestor " Adam, Eve " mentioned in " Holy Bible " is eaten, therefore English is known as
" PassionFruit ", means " love, passion, the fruit of enthusiasm ".
Passion fruit belongs to diclinism, and for female flower as a result, male flower is used to pollinate, its pollination medium is mainly honeybee and insect,
Also artificial pollination can be passed through.The passion fruit of spring plantation, can bloom, September can receive fruit July then.After second year, every year
Start April to bloom, 5-6 batches can be opened within 1 year, every batch of flower is separated by 20 days, is needed from blooming fruit maturation 60-70 days, and fruit is received
Obtain 4 wheat harvesting periods.
The propagation method of passion fruit mainly has two kinds, one kind be with the direct seed propagation of seed, it is another then be with climing bar
Cutting propagation.70-80 plants of main stems are planted per acre, and main stem separates lateral bine and climbed on frame step by step prolongs result.Passion fruit is every year per acre
Yield reach 1-1.5 tons, high yield up to more than 2 tons.
Passion fruit originates in South America and belongs to perennial evergreen climber liana tropical fruit (tree), because its fruit juice has guava, awns
The fragrance of the various fruits such as fruit, banana and gain the name.Passion fruit is adaptable, growth is vigorous, Resistant, goes into operation early, yield is high, product
Matter is good, and storage tolerance, market are wide, high financial profit, there is the laudatory title of " money tree " abroad.Passion fruit has the strong of various fruits
Fragrance, and very high nutritive value, can not only eat and fruit drink processed as fresh fruit, while be also with processing jam, jelly, ice river in Henan Province
Fruit is used in leaching, cake, the optimal processing of biscuit.Passion fruit fruit juice is as a kind of pure natural " green " fruit drink with its color, smell and taste
All good and good health-care effect, suitable for people of all ages, righteous style and practice is rubbed the world.
Passion fruit（Passiflora edulis Sims）, scientific name passionflower, Passifloraceae, Passiflora, for the torrid zone,
Subtropical zone perennial evergreen liana berry tree.Because its fruit juice gives out a kind peach, strawberry, lemon, mango, pineapple, banana, stone
The fragrance of the over one hundred kind of fruit such as pomegranate, smoked plum and passion fruit of gaining the name.Current plantation passion fruit pest and disease damage is serious, i.e., after passion fruit plantation
Butt rot；The passion fruit plant pest and disease damage outburst survived is serious, causes largely to use pesticide to be prevented；Passion fruit plant fruit
Honest fruit is serious, and yield declines.The self-contained virus of seedling of passion fruit plant, the phenomenon for causing passion fruit plantation obstacle to produce
It is urgently to be resolved hurrily.Therefore, establishing a kind of effective development of the passion fruit detoxic seedling cultural method for passion fruit industry has
Important meaning.
The content of the invention
It is an object of the invention to provide a kind of cultural method of passion fruit detoxic seedling, it is intended to solves above-mentioned technology and asks
Topic.
The present invention is achieved in that a kind of cultural method of passion fruit detoxic seedling, and the cultural method includes following step
Suddenly：
A, healthy passion fruit plant stem apex is chosen, using sterile water wash plant stem apex surface 4-6 times, plant stem apex is placed in and is disappeared
Soaking disinfection 12min ~ 18min in venom, is placed in 40 DEG C~47 DEG C water after immersion treatment 48min~70min, then by plant
Stem apex, which is placed in thimerosal, soaks the 15min ~ 20min that disappears；
B, the plant stem apex after pre-treatment is seeded to progress cellar culture 18d-23d on basal medium, and newborn axillary bud is taken
Go out to be seeded to again on basal medium and carry out cellar culture 15d-20d；
C, clip 5~8cm segment explants on healthy and strong disease-free plant, soak 3~5min in liquid detergent, then
Rinse 1h with tap water flowing water, aseptically, with ethanol wash 30s, reuse 0.1% mercuric chloride solution sterilizing 10~
12min, it is spare with aseptic water washing 3~5 times；
D, newly sprouting after the culture of basis, carries out virus-free culture, obtained tissue-cultured seedling carries out heat treatment 3d- at 40 DEG C~45 DEG C
After 6d, newly sprout and strip the stem apex of 0 .5cm, stem apex is placed in soaking disinfection 15min ~ 20min in thimerosal, clear with sterile water
Wash stem apex 3-5 times, stem apex is seeded on virus-free culture base and carries out cellar culture 15d-20d；By obtained tissue-cultured seedling at 40 DEG C
After carrying out heat treatment 3d-6d at~45 DEG C, newborn axillary bud strips the stem apex of 0 .2cm, and stem apex is placed in soaking disinfection in thimerosal
7min ~ 12min, with sterile water wash stem apex 5-7 times, then stem apex is seeded on virus-free culture base and carries out cellar culture 20d-
25d；
E, the passion fruit detoxic seedling after virus-free culture is seeded in propagation tissue-culturing rapid propagation culture medium, according to conventional tissue culture method
Carry out propagation tissue-culturing rapid propagation culture 25d-30d；
F, breed the passion fruit detoxic seedling after tissue-culturing rapid propagation culture to be seeded in root media, according to conventional tissue culture method into
Row culture of rootage 35d-45d.
The present invention further technical solution be：The ratio of each component is in thimerosal described in step A：By quality hundred
Fraction meter, the .04% of the .015% of the .004% of 0 .002% of antibiotic~0,0 .005% of hymexazol~0,0 .02% of methenamine~0,
The .02% of the .08% of 0 .06% of propamocarb~0,0 .01% of cresol sulfonic acid~0, surplus is water.
The present invention further technical solution be：Basal medium formulation described in step B is：By mass percentage,
The .015% of the .004% of the .0% of sucrose 2%~3%, 0 .8% of agar~1,0 .002% of antibiotic~0,0 .005% of hymexazol~0, crow
The .02% of the .08% of the .04% of 0 .02% of Lip river tropine~0,0 .06% of propamocarb~0,0 .01% of cresol sulfonic acid~0, zinc chloride
The .07 of 0 .05% of 0 .06% of 0 .3% of 0 .01%~0 .02%, MS+6-BA~0 .4%, NAA~0 .08%, IAA~0 %, it is remaining
Measure as water.
The beneficial effects of the invention are as follows：It is easy to operate, available for the industrialized production of large batch of passion fruit detoxic seedling, together
When detoxic seedling plantation after, effectively reduce passion fruit plant disease rate, compared with passion fruit seed of the routine without detoxification, the present invention
Passion fruit detoxic seedling plantation after incidence be only 2%, substantially increase the economic benefit of passion fruit.
Embodiment
A kind of cultural method of passion fruit detoxic seedling provided by the invention, details are as follows for it：
Step A, chooses healthy passion fruit plant stem apex, and using sterile water wash plant stem apex surface 4-6 times, plant stem apex is put
Soaking disinfection 12min ~ 18min in thimerosal, is placed in 40 DEG C~47 DEG C water after immersion treatment 48min~70min, then will
Plant stem apex, which is placed in thimerosal, soaks the 15min ~ 20min that disappears.
The ratio of each component is in the thimerosal：By mass percentage, 0 .004% of antibiotic, hymexazol
0.015%th, 0 .04% of methenamine, 0 .08% of propamocarb, 0 .02% of cresol sulfonic acid, surplus is water.
Step B, the plant stem apex after pre-treatment are seeded to progress cellar culture 18d-23d on basal medium, will be new
Raw axillary bud, which takes out to be seeded to again on basal medium, carries out cellar culture 15d-20d；The basal medium formulation is：Press
Mass percentage, sucrose 2%, 0 .8% of agar, 0 .004% of antibiotic, evil are mould
0 .015% of spirit, 0 .04% of methenamine, 0 .08% of propamocarb, 0 .02% of cresol sulfonic acid, 0 .02% of zinc chloride,
MS+6-BA0 .3%, 0 .05% of NAA 0 .06%, IAA, surplus is water.
Step C, clip 5~8cm segment explants on healthy and strong disease-free plant, soak 3 in liquid detergent~
5min, then 1h is rinsed with tap water flowing water, aseptically, with ethanol wash 30s, reuse 0.1% mercuric chloride solution
Sterilize 10~12min, spare with aseptic water washing 3~5 times；At the noon of fair weather or afternoon healthy and strong disease-free
Plant on the tender stem section of clip 5cm segments, each stem section containing 2 section, remove the blade on cardinal extremity section, stay the 1 of section
Piece leaf, soaks 3min in the liquid detergent stirred evenly, then rinses 1h with tap water flowing water, aseptically, with 75% alcohol
30s is washed, reuses 0.1% mercuric chloride solution sterilizing 10min, it is spare with aseptic water washing 3 times.
Step D, newly sprouting after the culture of basis, carries out virus-free culture, and obtained tissue-cultured seedling carries out at 40 DEG C~45 DEG C
After being heat-treated 3d-6d, newly sprout and strip the stem apex of 0 .5cm, stem apex is placed in soaking disinfection 15min ~ 20min in thimerosal, uses
Sterile water wash stem apex 3-5 times, stem apex is seeded on virus-free culture base and carries out cellar culture 15d-20d；The tissue culture that will be obtained
After seedling carries out heat treatment 3d-6d at 40 DEG C~45 DEG C, newborn axillary bud strips the stem apex of 0 .2cm, and stem apex is placed in thimerosal
Soaking disinfection 7min ~ 12min, with sterile water wash stem apex 5-7 times, then stem apex is seeded on virus-free culture base carry out it is conventional
Cultivate 20d-25d.
The virus-free culture based formulas is：By mass percentage, sucrose 3%, 0 .8% of agar, 0 .001% of antibiotic,
0 .002% of hymexazol, 0 .005% of methenamine, 000 .06% of .06%, NAA of .004%, MS+6-BA of propamocarb,
0 .02% of IAA, 0 .02% of vitamin B1,0 .02% of vitamin B2,0 .03% of vitamin B6, Vitamin B9 0 .02%, it is remaining
Measure as water.
Step E, the passion fruit detoxic seedling after virus-free culture is seeded in propagation tissue-culturing rapid propagation culture medium, according to routine
Tissue culture method carries out propagation tissue-culturing rapid propagation culture 25d-30d.
The propagation tissue-culturing rapid propagation culture medium prescription is：By mass percentage, sucrose 2%, agar 0 .8%, MS+6-
BA0 .08%, 0 .05% of NAA 0 .06%, IAA, 0 .04% of vitamin B1,0 .03% of vitamin B2, vitamin B6
0.03%th, 0 .02% of Vitamin B9, surplus are water.
Step F, breeds the passion fruit detoxic seedling after tissue-culturing rapid propagation culture and is seeded in root media, according to conventional group
Culture method carries out culture of rootage 35d-45d.
The prescription of rooting medium is：By mass percentage, sucrose 3%, 1 .0%, MS+6-BA 0 of agar
.12%, NAA0 .08%, 0 .4% of IAA 0 .07%, CCC, 0 .06% of vitamin B1,0 .05% of vitamin B2, dimension life
Plain B60 .05%, 0 .04% of Vitamin B9, surplus is water.
The prescription of rooting medium is：By mass percentage, 2 .5% of sucrose, agar 0 .9%, MS+6-BA
0.10%th, 000 .35% of .06%, CCC of .08%, IAA of NAA, 0 .05% of vitamin B1,0 .04% of vitamin B2, vitamin
0 .04% of B6,0 .03% of Vitamin B9, surplus is water.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (3)
1. a kind of cultural method of passion fruit detoxic seedling, it is characterised in that the cultural method comprises the following steps：
A, healthy passion fruit plant stem apex is chosen, using sterile water wash plant stem apex surface 4-6 times, plant stem apex is placed in and is disappeared
Soaking disinfection 12min ~ 18min in venom, is placed in 40 DEG C~47 DEG C water after immersion treatment 48min~70min, then by plant
Stem apex, which is placed in thimerosal, soaks the 15min ~ 20min that disappears；
B, the plant stem apex after pre-treatment is seeded to progress cellar culture 18d-23d on basal medium, and newborn axillary bud is taken
Go out to be seeded to again on basal medium and carry out cellar culture 15d-20d；
C, clip 5~8cm segment explants on healthy and strong disease-free plant, soak 3~5min in liquid detergent, then
Rinse 1h with tap water flowing water, aseptically, with ethanol wash 30s, reuse 0.1% mercuric chloride solution sterilizing 10~
12min, it is spare with aseptic water washing 3~5 times；
D, newly sprouting after the culture of basis, carries out virus-free culture, obtained tissue-cultured seedling carries out heat treatment 3d- at 40 DEG C~45 DEG C
After 6d, newly sprout and strip the stem apex of 0 .5cm, stem apex is placed in soaking disinfection 15min ~ 20min in thimerosal, clear with sterile water
Wash stem apex 3-5 times, stem apex is seeded on virus-free culture base and carries out cellar culture 15d-20d；By obtained tissue-cultured seedling at 40 DEG C
After carrying out heat treatment 3d-6d at~45 DEG C, newborn axillary bud strips the stem apex of 0 .2cm, and stem apex is placed in soaking disinfection in thimerosal
7min ~ 12min, with sterile water wash stem apex 5-7 times, then stem apex is seeded on virus-free culture base and carries out cellar culture 20d-
25d；
E, the passion fruit detoxic seedling after virus-free culture is seeded in propagation tissue-culturing rapid propagation culture medium, according to conventional tissue culture method
Carry out propagation tissue-culturing rapid propagation culture 25d-30d；
F, breed the passion fruit detoxic seedling after tissue-culturing rapid propagation culture to be seeded in root media, according to conventional tissue culture method into
Row culture of rootage 35d-45d.
2. cultural method according to claim 1, it is characterised in that the ratio of each component in the thimerosal described in step A
Example be：By mass percentage, the .015% of the .004% of 0 .002% of antibiotic~0,0 .005% of hymexazol~0, methenamine
The .02% of the .08% of 0.02%~0 .04%, 0 .06% of propamocarb~0,0 .01% of cresol sulfonic acid~0, surplus is water.
3. cultural method according to claim 2, it is characterised in that the basal medium formulation described in step B is：By matter
Measure percentage meter, the .004% of the .0% of sucrose 2%~3%, 0 .8% of agar~1,0 .002% of antibiotic~0,0 .005% of hymexazol~
The .08% of the .04% of 0 .015%, 0 .02% of methenamine~0,0 .06% of propamocarb~0,0 .01% of cresol sulfonic acid~
0.02%th, 0 .01% of zinc chloride~0 .02%, MS+6-BA, 0 .3%~0 .4%, NAA, 0 .06%~0 .08%, IAA, 0 .05%
~0 .07 %, surplus are water.
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| CN201711310783.3A CN107896993A (en) | 2017-12-11 | 2017-12-11 | The culture technique of passion fruit detoxic seedling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109874676A (en) * | 2019-04-11 | 2019-06-14 | 广西凭祥桂商现代农业投资有限公司 | A kind of fast breeding method of passion fruit detoxic seedling |
-
2017
- 2017-12-11 CN CN201711310783.3A patent/CN107896993A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109874676A (en) * | 2019-04-11 | 2019-06-14 | 广西凭祥桂商现代农业投资有限公司 | A kind of fast breeding method of passion fruit detoxic seedling |
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