CN105393791A - Preparation method for living grafted Ganodermalucidum bonsais - Google Patents

Preparation method for living grafted Ganodermalucidum bonsais Download PDF

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CN105393791A
CN105393791A CN201510757878.4A CN201510757878A CN105393791A CN 105393791 A CN105393791 A CN 105393791A CN 201510757878 A CN201510757878 A CN 201510757878A CN 105393791 A CN105393791 A CN 105393791A
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composts
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孔云
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Wuxi County Yunxiang Edible Shares Professional Cooperatives
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Wuxi County Yunxiang Edible Shares Professional Cooperatives
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D1/00Fertilisers containing potassium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Mycology (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention relates to a preparation method for living grafted Ganodermalucidum bonsais. According to the preparation method for the living grafted Ganodermalucidum bonsais, the quality of the Ganodermalucidum bonsais prepared by the method for the living grafted the Ganodermalucidum bonsais can be improved; compared with the prior art, the Ganodermalucidum bonsais prepared by the preparation method for the living grafted Ganodermalucidum bonsais have the advantages that: the number of complete Ganodermalucidum spores in each gram of spore power can be increased by 31.40%; and the wall-broken rate of wall-broken Ganodermalucidum spore power can be increased by 22.31%.

Description

The preparation method of live body grafting glossy ganoderma dish garden
Technical field
The present invention relates to a kind of making method for its potted landscape, be specially a kind of preparation method of live body grafting glossy ganoderma dish garden.
Background technology
Glossy ganoderma, also known as Lin Zhongling, celestial grass, refreshing grass, auspicious grass, is Basidiomycetes Polyporaceae Ganoderma fungi.Glossy ganoderma have strengthen the body resistance to consolidate the constitution, invigorating qi for tranquilization, relieving cough and asthma effect, be the Chinese tradition valuable ingredient of traditional Chinese medicine having thousands of years medicinal histories, there is very high medical value.
Existing glossy ganoderma dish garden is mostly that the dry product glossy ganoderma crossed through artificial treatment on streamline is put together, the ornaments of dry product both not angry, there is no vitality, more without health value and edible medical value.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of preparation method of live body grafting glossy ganoderma dish garden.
For achieving the above object, the technical solution used in the present invention is as follows:
A preparation method for live body grafting glossy ganoderma dish garden, its concrete steps are:
A. spice: take composts or fertilisers of cultivating according to culture medium prescription, mixes, and adding water to water content is 62-64%; Described medium is composed of the following components by weight: cotton seed hulls 25-30 part, stalk section 15-25 part, wheat bran 6-15 part, corncob section 15-25 part, land plaster 3 parts, 0.5 part, potash, 2 parts, brown sugar, water 20-30 part;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, cultivation;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, and ganoderma lucidum mycelium be loaded in flowerpot and be cultured to fruit body medium well;
D. grafting: the fruit body in transfer room is inserted in the fruit body in flowerpot as skewer fringe;
E. potted landscape management: moulding, and modify.
Further, the concrete steps of the preparation method of described live body grafting glossy ganoderma dish garden are:
A. spice: take composts or fertilisers of cultivating according to culture medium prescription, mixes, and adding water to water content is 63%; Described medium is composed of the following components by weight: cotton seed hulls 28 parts, stalk section 20 parts, 9 parts, wheat bran, corncob section 20 parts, land plaster 3 parts, 0.5 part, potash, 2 parts, brown sugar, 25 parts, water;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, cultivation;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, and ganoderma lucidum mycelium be loaded in flowerpot and be cultured to fruit body medium well;
D. grafting: the fruit body in transfer room is inserted in the fruit body in flowerpot as skewer fringe;
E. potted landscape management: moulding, and modify.
Glossy ganoderma, also known as Lin Zhongling, celestial grass, refreshing grass, auspicious grass, is Basidiomycetes Polyporaceae Ganoderma fungi.Glossy ganoderma have strengthen the body resistance to consolidate the constitution, invigorating qi for tranquilization, relieving cough and asthma effect, be the Chinese tradition valuable ingredient of traditional Chinese medicine having thousands of years medicinal histories, there is very high medical value.
Glossy ganoderma can launch out conidial powder growing to the stage of ripeness, and the medical value of conidial powder, than glossy ganoderma height decades of times, is the elite of glossy ganoderma.Conidial powder can purify the air of a room, after people absorbs, can tranquilizing and allaying excitement, and very useful to health; Can film cover bag collection enjoy when the ripe conidial powder of new sesame is flown away in a large number.Therefore have colourful fresh and alive glossy ganoderma dish garden, be not only room or office space and make something perfect even more perfect, but also can play and allow people's spirit and QI cleaning, contribute to one's peace of mind of inner tranquility, develop immunitypty, regulates effect of health each side function.
Further, the length of stalk section described in steps A is 0.4-1.2cm, and the length of corncob section is 0.4-0.6mm.Be conducive to stalk and corncob water suction like this, avoid granule to puncture bacterium bag simultaneously and cause hole to pollute.
Further, described spice is mixed cotton seed hulls, stalk section, wheat bran, corncob section and land plaster, adds after water-soluble for brown sugar formation brown sugar water, then be 62-64% with water sprinkling composts or fertilisers of cultivating to its total moisture content.Such moisture is not easy to scatter and disappear, and improves medium moisture capacity.
Further, described composts or fertilisers of cultivating repeatedly carries out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing in ventilation, then continues to spray, until reach required water content.This operation is also conducive to moisture and fully enters medium inside, and moisture is not easy to scatter and disappear, and improves medium moisture capacity.
Further, the pH spraying water used is 5.5-6.Under this pH environment, glossy ganoderma well-grown.
Further, sterilizing described in step B be by charging after pocket in 100 DEG C of sterilizing 8-12 hour, or in 120 DEG C of sterilizing 1-2 hour.
Further, the temperature of cultivating described in step B and step C is 25-27 DEG C, relative moisture is 80-85%.
Further, in incubation described in step C, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g.Triacontanol is a kind of natural Long carbon chain plant growth regulator, chemical name N-triacontanol, structural formula CH 3(CH 2) 28cH 2oH, also known as melissyl alcohol, is the natural biological product of purification from honeybee wax, to person poultry harmless and side effect, and environmentally safe.Use it for the growth regulating of glossy ganoderma, the incubation time of lucidum strain can be shortened, enhance productivity.
The invention has the beneficial effects as follows:
The glossy ganoderma dish garden adopting the method for the invention to prepare, significantly improves the quality of glossy ganoderma dish garden.Compared with prior art, the complete Reishi sporule number contained in every gram of conidial powder of the glossy ganoderma dish garden adopting the method for the invention to prepare improves 31.40%; The sporoderm-broken rate of ganoderma spove powder improves 22.31%.
Embodiment
In order to make the object, technical solutions and advantages of the present invention clearly, below embodiments of the invention are described in detail.
Embodiment 1
The preparation method of live body grafting glossy ganoderma dish garden, its concrete steps are:
A. spice: be stalk section 25kg, wheat bran 6kg, the length of 0.4cm by cotton seed hulls 30kg, length be that 0.6mm corncob section 15kg, land plaster 3kg and potash 0.5kg mix, add after water-soluble for brown sugar 2kg 30kg is formed brown sugar water, then be 64% with water sprinkling composts or fertilisers of cultivating to its total moisture content that pH is 5.5; Spray composts or fertilisers of cultivating with water repeatedly to carry out, be sprayed to water droplet at every turn and gone out and namely stop, being placed to anhydrous oozing in ventilation, then continue to spray, until reach required water content;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, and in temperature be 27 DEG C, relative moisture cultivates under being 80% condition; Described sterilizing be by charging after pocket in 100 DEG C of sterilizings 8 hours;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, ganoderma lucidum mycelium is loaded in flowerpot in temperature be 25 DEG C, relative moisture is cultured to fruit body medium well under being 85% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g;
D. grafting: the fruit body in transfer room is inserted in the fruit body in flowerpot as skewer fringe;
E. potted landscape management: moulding, and modify.
Embodiment 2
The preparation method of live body grafting glossy ganoderma dish garden, its concrete steps are:
A. spice: be stalk section 15kg, wheat bran 15kg, the length of 1.2cm by cotton seed hulls 25kg, length be that 0.4mm corncob section 25kg, land plaster 3kg and potash 0.5kg mix, add after water-soluble for brown sugar 2kg 20kg is formed brown sugar water, then be 62% with water sprinkling composts or fertilisers of cultivating to its total moisture content that pH is 6; Spray composts or fertilisers of cultivating with water repeatedly to carry out, be sprayed to water droplet at every turn and gone out and namely stop, being placed to anhydrous oozing in ventilation, then continue to spray, until reach required water content;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, and in be 25 DEG C in temperature, relative moisture cultivates under being 85% condition; Described sterilizing be by charging after pocket in 100 DEG C of sterilizings 12 hours;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, ganoderma lucidum mycelium is loaded in flowerpot in 27 DEG C, relative moisture is cultured to fruit body medium well under being 80% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g;
D. grafting: the fruit body in transfer room is inserted on step C resulting bottle entity as skewer fringe;
E. potted landscape management: moulding, and modify.
Embodiment 3
The preparation method of live body grafting glossy ganoderma dish garden, its concrete steps are:
A. spice: be stalk section 20kg, wheat bran 9kg, the length of 0.8cm by cotton seed hulls 28kg, length be that 0.5mm corncob section 20kg, land plaster 3kg and potash 0.5kg mix, add after water-soluble for brown sugar 2kg 25kg is formed brown sugar water, then be 63% with water sprinkling composts or fertilisers of cultivating to its total moisture content that pH is 5.8; Spray composts or fertilisers of cultivating with water repeatedly to carry out, be sprayed to water droplet at every turn and gone out and namely stop, being placed to anhydrous oozing in ventilation, then continue to spray, until reach required water content;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, and in temperature be 26 DEG C, relative moisture cultivates under being 84% condition; Described sterilizing be by charging after pocket in 100 DEG C of sterilizings 10 hours;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, ganoderma lucidum mycelium is loaded in flowerpot in 26 DEG C, relative moisture is cultured to fruit body medium well under being 82% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g;
D. grafting: the fruit body in transfer room is inserted in the fruit body in flowerpot as skewer fringe;
E. potted landscape management: moulding, and modify.
Embodiment 4
The preparation method of live body grafting glossy ganoderma dish garden, its concrete steps are:
A. spice: be stalk section 18kg, wheat bran 10kg, the length of 1.0cm by cotton seed hulls 21kg, length be that 0.5mm corncob section 18kg, land plaster 3kg and potash 0.5kg mix, add after water-soluble for brown sugar 2kg 24kg is formed brown sugar water, then be 62% with water sprinkling composts or fertilisers of cultivating to its total moisture content that pH is 5.6; Spray composts or fertilisers of cultivating with water repeatedly to carry out, be sprayed to water droplet at every turn and gone out and namely stop, being placed to anhydrous oozing in ventilation, then continue to spray, until reach required water content;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, and in temperature be 26 DEG C, relative moisture cultivates under being 81% condition; Described sterilizing be by charging after pocket in 120 DEG C of sterilizings 1 hour;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, ganoderma lucidum mycelium is loaded in flowerpot in 27 DEG C, relative moisture is cultured to fruit body medium well under being 82% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g;
D. grafting: the fruit body in transfer room is inserted in the fruit body in flowerpot as skewer fringe;
E. potted landscape management: moulding, and modify.
Embodiment 5
The preparation method of live body grafting glossy ganoderma dish garden, its concrete steps are:
A. spice: be stalk section 13kg, wheat bran 12kg, the length of 0.6cm by cotton seed hulls 23kg, length be that 0.6mm corncob section 23kg, land plaster 3kg and potash 0.5kg mix, add after water-soluble for brown sugar 2kg 24kg is formed brown sugar water, then be 64% with water sprinkling composts or fertilisers of cultivating to its total moisture content that pH is 5.7; Spray composts or fertilisers of cultivating with water repeatedly to carry out, be sprayed to water droplet at every turn and gone out and namely stop, being placed to anhydrous oozing in ventilation, then continue to spray, until reach required water content;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, and in temperature be 25 DEG C, relative moisture cultivates under being 83% condition; Described sterilizing be by charging after pocket in 120 DEG C of sterilizings 2 hours;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, ganoderma lucidum mycelium is loaded in flowerpot in 26 DEG C, relative moisture is cultured to fruit body medium well under being 84% condition; In incubation, fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g;
D. grafting: the fruit body in transfer room is inserted in the fruit body in flowerpot as skewer fringe;
E. potted landscape management: moulding, and modify.
Embodiment 6
With publication number for method described in CN103688750B makes live body glossy ganoderma dish garden.Its concrete steps are:
(1) ganoderma lucidum mycelium cultivated by bacterium bag
A. live body glossy ganoderma growth culture medium raw material and mass fraction be: weed tree sawdust 25%, wood particle 50%, wheat bran 15%, corn flour 5%, analysis for soybean powder 3%, gypsum 1% and brown sugar 1%;
B. first wood particle clear water is soaked 24 hours when preparing planting material, wood chip, wheat bran, corn flour, analysis for soybean powder, gypsum raw material are mixed thoroughly, mix in raw meal and mix thoroughly after brown sugar is water-soluble, the water content of raw meal is 63%, and pH value is 6; Adopt the polypropylene plastics pocket of 17cm × 33cm × 0.045cm raw meal to be packed, limit pack limit compacting, degree of tightness is suitable for, sack loudspeaker choma and sponge gag, then carries out medium sterilization, inoculation, cultivation;
C. sterilizing immediately after medium pack, 121 DEG C keep 2 hours, take out after sterilizing, inoculate, inoculate and undertaken by sterile working when medium temperature is cooled to below 30 DEG C, labelled after inoculation, indicate lucidum variety;
D. moved in culturing room by vaccinated bacterium bag, be emitted on culturing rack top fermentation, control culturing room's temperature 30 DEG C, relative air humidity is 65 DEG C, and light culture checked every 5 days and prevents and treats living contaminants, after mycelium sends out purseful, loads flowerpot and cultivates.
(2) ganoderma lucidum fruitbody cultivated by flowerpot
A. flowerpot is prepared: select the flowerpot of suitable size and moulding to be used for cultivating ganoderma;
B. flowerpot sterilization: clean inside and outside flowerpot with the hydrogen peroxide that mass fraction is 5%;
C. the in-built ganoderma lucidum mycelium of flowerpot: full mycelial Ganoderma Lucidum bag will be sent out and go to lose a bag shell, and culture be loaded in flowerpot, compacting, pressing, first cover preservative film, then cover leatheroid, draw two-lines at the fruiting mouth place of preservative film setting, long 2cm, across, a bamboo let is inserted at center, long 8cm, diameter 0.3cm, insert dark 6cm, it is the hole of 3cm that leatheroid digs a diameter, and the bamboo let of fruiting mouth is aimed at hole; Then cover live body glossy ganoderma growing cover, control temperature is 33 DEG C, light culture;
D. tap reason is gone out: keep mushroom chamber venting good, when the former base of glossy ganoderma produces around the bamboo let of branch mouth of fixing a point out, black film above live body glossy ganoderma growing cover opens the Dong Jiu that diameter is 5cm, control temperature is at 32 DEG C, humidity is 95%, and intensity of illumination is 1000LX, promotes glossy ganoderma long handle, and stem grow tall, sturdy, and to light source direction bends;
When handle grows to 10cm, all remove the black film on cover, it is the hole of 10cm that the tunica albuginea of cover top opens a diameter, and keep temperature to be 32 DEG C, humidity is 95%, and intensity of illumination is 5000LX, promotes glossy ganoderma leaf growth;
When handle grows to 6cm, get the long 6cm of blade that bacterium bag is cultivated, the glossy ganoderma 1 of the long 8cm of handle does scion, grafting on the blade of bonsai lucid ganoderma, contact first cuts 1 cutter with sharp blade by flat for epidermis, bacterium autumn and scion are put on grafting point, scion and family's contact is fixed with bamboo let, again live body glossy ganoderma growing cover is covered, forbid before not surviving after grafting to spray water, treat that the mycelia on scion and grafting point is twisted joint firmly, scion growing point restoration ecosystem, the long 4cm of blade that bacterium bag is cultivated is got again after glossy ganoderma survives, 1 glossy ganoderma of the long 6cm of handle carries out grafting, namely by normal management after grafting survival,
E. potted landscape management: after glossy ganoderma grafting survival main body is shaped substantially, control temperature is 25 DEG C, and humidity is 85%, and intensity of illumination is 3000LX.
Embodiment 7
Use the tally of 25 middle lattice × 16 little lattice, calculate the sporoderm-broken rate of complete Reishi sporule number and the ganoderma spove powder contained in every gram of conidial powder of the live body glossy ganoderma dish garden (being respectively experimental group 1,2,3,4,5) of embodiment 1-5 making and the live body glossy ganoderma dish garden (control group) of embodiment 6 making.Result is as shown in the table:
Remarks: the sporoderm-broken rate of the complete Reishi sporule number contained in every gram of conidial powder, ganoderma spove powder measures according to People's Republic of China's professional standard " GB/T29344-2012 lucidum spore powder is gathered and process technology specification ".
As seen from the above table, compared with control group, the complete Reishi sporule number contained in every gram of conidial powder of glossy ganoderma dish garden prepared by experimental group improves 31.40%; The sporoderm-broken rate of ganoderma spove powder improves 22.31%.Prove thus, adopt the method for the invention to prepare glossy ganoderma dish garden, significantly improve the quality of glossy ganoderma dish garden.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, those of ordinary skill in the art is to be understood that, can modify to technical scheme or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.

Claims (9)

1. the preparation method of live body grafting glossy ganoderma dish garden, is characterized in that, concrete steps are:
A. spice: take composts or fertilisers of cultivating according to culture medium prescription, mixes, and adding water to water content is 62-64%; Described medium is composed of the following components by weight: cotton seed hulls 25-30 part, stalk section 15-25 part, wheat bran 6-15 part, corncob section 15-25 part, land plaster 3 parts, 0.5 part, potash, 2 parts, brown sugar, water 20-30 part;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, cultivation;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, and ganoderma lucidum mycelium be loaded in flowerpot and be cultured to fruit body medium well;
D. grafting: the fruit body in transfer room is inserted in the fruit body in flowerpot as skewer fringe;
E. potted landscape management: moulding, and modify.
2. preparation method according to claim 1, is characterized in that, concrete steps are:
A. spice: take composts or fertilisers of cultivating according to culture medium prescription, mixes, and adding water to water content is 63%; Described medium is composed of the following components by weight: cotton seed hulls 28 parts, stalk section 20 parts, 9 parts, wheat bran, corncob section 20 parts, land plaster 3 parts, 0.5 part, potash, 2 parts, brown sugar, 25 parts, water;
B. the composts or fertilisers of cultivating mixed is carried out packing, sterilizing, inoculation, cultivation;
C. will send out full mycelial Ganoderma Lucidum bag to go to lose shell bag, and ganoderma lucidum mycelium be loaded in flowerpot and be cultured to fruit body medium well;
D. grafting: the fruit body in transfer room is inserted in the fruit body in flowerpot as skewer fringe;
E. potted landscape management: moulding, and modify.
3. preparation method according to claim 1, is characterized in that, the length of stalk section described in steps A is 0.4-1.2cm, and the length of corncob section is 0.4-0.6mm.
4. preparation method according to claim 1, it is characterized in that: described spice is mixed cotton seed hulls, stalk section, wheat bran, corncob section and land plaster, add after water-soluble for brown sugar formation brown sugar water, then be 62-64% with water sprinkling composts or fertilisers of cultivating to its total moisture content.
5. preparation method according to claim 4, is characterized in that: described water sprays composts or fertilisers of cultivating repeatedly to carry out, and has been sprayed to water droplet at every turn and has gone out and namely stop, being placed to anhydrous oozing, then continuing to spray, until reach required water content in ventilation.
6. the preparation method according to claim 4 or 5, is characterized in that: the pH spraying water used is 5.5-6.
7. preparation method according to claim 1, is characterized in that: sterilizing described in step B be by charging after pocket in 100 DEG C of sterilizing 8-12 hour, or in 120 DEG C of sterilizing 1-2 hour.
8. preparation method according to claim 1, is characterized in that: the temperature of cultivating described in step B and step C is 25-27 DEG C, relative moisture is 80-85%.
9. preparation method according to claim 1, is characterized in that: in incubation described in step C, and fruit body grows to two maturations and with time medium rare, on blade face, spray tridecanol, concentration is respectively 0.5mg/kg and 1mg/kg, and consumption is respectively 5g and 8g.
CN201510757878.4A 2015-11-09 2015-11-09 Preparation method for living grafted Ganodermalucidum bonsais Pending CN105393791A (en)

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CN106479903A (en) * 2016-10-14 2017-03-08 北京京诚生物科技有限公司 A kind of manufacture method of live body glossy ganoderma dish garden
CN110521490A (en) * 2016-10-14 2019-12-03 北京京诚生物科技有限公司 Lucidum spore powder breeding method

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106479903A (en) * 2016-10-14 2017-03-08 北京京诚生物科技有限公司 A kind of manufacture method of live body glossy ganoderma dish garden
CN106479903B (en) * 2016-10-14 2019-10-25 北京京诚生物科技有限公司 A kind of production method of living body glossy ganoderma dish garden
CN110521490A (en) * 2016-10-14 2019-12-03 北京京诚生物科技有限公司 Lucidum spore powder breeding method

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Application publication date: 20160316