CN103299822A - Schizophyllum commune Fr high-yield fruiting body strain and three-dimensional ecology-returning cultivation method - Google Patents
Schizophyllum commune Fr high-yield fruiting body strain and three-dimensional ecology-returning cultivation method Download PDFInfo
- Publication number
- CN103299822A CN103299822A CN2012100604055A CN201210060405A CN103299822A CN 103299822 A CN103299822 A CN 103299822A CN 2012100604055 A CN2012100604055 A CN 2012100604055A CN 201210060405 A CN201210060405 A CN 201210060405A CN 103299822 A CN103299822 A CN 103299822A
- Authority
- CN
- China
- Prior art keywords
- bacterial strain
- bacterium
- bag
- cultivation
- schizophyllum commune
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000222481 Schizophyllum commune Species 0.000 title claims abstract description 30
- 238000012364 cultivation method Methods 0.000 title claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims abstract description 42
- 238000011081 inoculation Methods 0.000 claims abstract description 9
- 238000012216 screening Methods 0.000 claims abstract description 5
- 230000001580 bacterial effect Effects 0.000 claims description 35
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 239000002023 wood Substances 0.000 claims description 19
- 235000013399 edible fruits Nutrition 0.000 claims description 8
- 229920000742 Cotton Polymers 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 235000013312 flour Nutrition 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- 235000015099 wheat brans Nutrition 0.000 claims description 7
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 6
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 6
- 239000011425 bamboo Substances 0.000 claims description 6
- -1 polyethylene Polymers 0.000 claims description 6
- 240000007154 Coffea arabica Species 0.000 claims description 5
- 238000002474 experimental method Methods 0.000 claims description 5
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 4
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 4
- 239000004571 lime Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims description 3
- 235000007460 Coffea arabica Nutrition 0.000 claims description 3
- 238000006424 Flood reaction Methods 0.000 claims description 3
- 108091092584 GDNA Proteins 0.000 claims description 3
- 240000003553 Leptospermum scoparium Species 0.000 claims description 3
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 240000003194 Sida rhombifolia Species 0.000 claims description 3
- 235000002834 Sida rhombifolia Nutrition 0.000 claims description 3
- 238000011109 contamination Methods 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 244000269722 Thea sinensis Species 0.000 claims description 2
- 239000002361 compost Substances 0.000 claims description 2
- 230000003203 everyday effect Effects 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 241001330002 Bambuseae Species 0.000 claims 4
- 238000001035 drying Methods 0.000 claims 2
- 235000016709 nutrition Nutrition 0.000 claims 2
- 230000035764 nutrition Effects 0.000 claims 2
- 230000005070 ripening Effects 0.000 claims 2
- 238000009423 ventilation Methods 0.000 claims 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 239000000853 adhesive Substances 0.000 claims 1
- 230000001070 adhesive effect Effects 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims 1
- 239000011505 plaster Substances 0.000 claims 1
- 238000004080 punching Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
- 230000035800 maturation Effects 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 description 6
- 241000233866 Fungi Species 0.000 description 4
- 241000222480 Schizophyllum Species 0.000 description 4
- 239000004567 concrete Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000011165 3D composite Substances 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 2
- 241000221198 Basidiomycota Species 0.000 description 2
- 240000001624 Espostoa lanata Species 0.000 description 2
- 235000009161 Espostoa lanata Nutrition 0.000 description 2
- 244000082204 Phyllostachys viridis Species 0.000 description 2
- 238000001354 calcination Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000561734 Celosia cristata Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 240000008397 Ganoderma lucidum Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000222479 Schizophyllaceae Species 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 241000746966 Zizania Species 0.000 description 1
- 235000002636 Zizania aquatica Nutrition 0.000 description 1
- 240000000038 Ziziphus mauritiana Species 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 210000001520 comb Anatomy 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 201000000498 stomach carcinoma Diseases 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a Schizophyllum commune Fr high-yield fruiting body strain and a three-dimensional ecology-returning cultivation method, which comprises the following steps of: 1. strain collection and domestication; 2. strain screening; 3. optimum cultivation season; 4. bacteria package production; 5. inoculation; 6. bacteria package culture; 7. maturation culture; and 8. three-dimensional ecology-returning cultivation.
Description
Technical field
The present invention is the domestication of edible and medical fungi bacterial strain and cultivation field.
Background technology
Schizophyllum commune (Schizophyllum commune Fr.), local name: chicken feather mushroom (Hubei), cockscomb bacterium (Hunan), tree flower (Shaanxi), white gill fungus, white ginseng, smallpox gill fungus (Yunnan), little bavin wild rice (Fujian), eight load bavins (common name).Be under the jurisdiction of mycota (Mycota), Eumycota (Eumycota), Basidiomycetes (Basidiomycetes), Agaricales (Agaricales), schizophyllum commune section (Schizophyllaceae), Schizophyllum (Schizophyllum).It is a kind of famous and precious wild edible and medical fungi of being born on the rotten wood of summer and autumn, and during the schizophyllum sporophores children, matter is tender delicious, has special strong fragrance, and Yunnan the with a long history of schizophyllum commune that search for food regarded it as the high-grade edible bacterium, and products export is arranged.The amino acid content of schizophyllum commune is very abundant, contains 18 seed amino acids, and the total amino acid content in the schizophyllum sporophores is 5.51%, and comprising necessary 8 seed amino acids of human body, and the ratio that essential amino acid accounts for total amino acid content is 40.83%.Studies show that schizophyllum commune has the multiple physiologically actives such as the immunity of organisms of raising, anti-inflammation, radioresistance, had good edible and medical value.Schizophyllum commune Fr polysaccharides has been developed to anti-tumor agent at present, is used for the treatment of in conjunction with chemicotherapy in the treatment of cervix cancer, cancer of the stomach and hepatitis B etc.Yet until today, the cultivation scale of schizophyllum commune is but still very little, and restricting factor mainly is that output is unstable because tame schizophyllum commune biological transformation ratio is low.There is no at present superior strain and return the report of ecological three-dimensional composite cultivation mode.
Summary of the invention
The present invention has been the domestication one strain schizophyllum commune high yield fruit body bacterial strain, and innovated the schizophyllum commune planting type of present report, first schizophyllum commune is back in the natural ecological environment, take full advantage of the regional environment in tea place, coffee garden, forest land, not only improved the unit soil availability, increased soil fertility, but also improved the tame output of schizophyllum commune, improved quality.
Schizophyllum commune GGHN08-26 returns in the ecological three-dimensional composite cultivation mode at " tea-schizophyllum commune ", and biological transformation ratio reaches 45.8%; The amino acid content that returns ecological schizophyllum commune is 17.805%, is 3 times of wild schizophyllum commune; The polyoses content that returns ecological schizophyllum commune is 24.1%, is 80 times of artificial cultivation schizophyllum commune.Particular content of the present invention comprises the following aspects: 1. wild strain is collected and domestication.A large amount of bacterial strains that open-air mushroom wood is separated at first confirm by the fruiting experiment, and then by mycelial growth rate determination test, anti-hybrid ability test and fruiting experiment, it is schizophyllum commune GGHN08-26 that final screening obtains bacterial strain.This bacterial strain picks up from Central Yunnan, host plant is that this bacterial strain of indian jujube Ziziphus mauritiana Lam.2. belongs to wood decay fungi, has stronger decomposition lignin, cellulosic ability, well-grown on the matrix that contains glucose, cellulose, hemicellulose and lignin also needs mineral element and a small amount of vitamins such as potassium, magnesium, calcium, phosphorus simultaneously.3. the best cultivation prescription of this bacterial strain is cotton seed hulls 45%, wood chip 30%, wheat bran 15%, corn flour 8%, lime 1%, sugar 1%.4. returning in the ecological cultivation process at first is arrangement groove face, trench digging, tea weeds are in the ranks processed clean, preferably with the weeds evulsion, tea tree in the ranks, coffee tree in the ranks or woodland open a wide 35cm, the ditch of dark 15cm.Next is that the bacterium bag is processed and put, the sack of bacterium bag is scratched with pocket knife, whole polyethylene (polypropylene) plastic sack is torn off, pollute if bacterium is surrounded by part, pollute part with hilt and remove, in the situation with good conditionsi, soak gdna contamination with limewash again, be horizontally placed on ditch after taking off bag, between the bacterium bag about spacing 2-3cm, after all the bacterium bag is put fully, fully pour water once.Earthing at last, treat that moisture all floods down between ditch after, use the fine earth earthing, fill up slit and bacterium bag surface, the thick 2-3cm of earthing, and water sufficient water at earthing.5. in the management of producing mushroom process, standard of plucking is that the schizophyllum commune cap is not carried out fully, and is best when color is milky, during harvesting, holds the stem base portion, and whole clump is taken.Adopted the later water spray of mushroom once every day during fruiting, and water spray is advisable with moistening table soil.After treating that the first damp mushroom fruiting fully, stop water spray, bacteria 8-10 days, then fully pouring water once went out the second damp mushroom.
Embodiment
1. the domestication breeding of bacterial strain:
The method of at first separating by mushroom wood obtains a large amount of bacterial classifications from the field, concrete measure: remove the fruit body on the mushroom wood, with apparatus such as cotton ball soaked in alcohol wiping mushroom wood, hand, scalpels, wiping is crossed the cotton balls of apparatus and light the calcination scalpel with fire, aglow rear cooling, the mushroom outer surface of wood skin of pruning gently and giving birth to fruit body, and then a calcination scalpel, after cooling, cutting the access of lower block of mushroom wood with the mushroom wood position of scalpel after peeling is equipped with in the centrifuge tube of PDA medium.
Then by the checking of purifying and fruiting experiment, concrete measure: the bacterial classification that separates of mushroom wood is taken back on the PDA medium that the laboratory is transferred to the bacterium of having gone out in the open air, can until bacterium colony is pure.To the composts or fertilisers of cultivating of the bacterium of having gone out, 25 ℃ of dark cultivations treat that mycelia covers with the bacterium bag and move to fruiting with bacterial classification inoculation behind the purifying
Mushroom, the higher bacterial strain of primary dcreening operation fruiting output.
Bacterial strain behind the primary dcreening operation is further sieved again, and, green mold etc. mould by anti-wood resists assorted test, and the screening anti-hybrid ability is strong, the bacterial strain that fruiting is stable.
By above test, the bacterial strain that finishing screen is selected is GGHN08-26.
2. best fruiting prescription screening
By the different formulations comparative test, final definite take cotton seed hulls, wood chip/bamboo bits, wheat bran, corn flour etc. as raw material, this bacterial strain has maximum output, and the concrete proportioning of each composition is: cotton seed hulls 45%, wood chip/bamboo bits 30%, wheat bran 15%, corn flour 8%, lime 1%, sugar 1%.
3. schizophyllum commune returns ecological cultivation
At first be arrangement groove face, trench digging, tea weeds are in the ranks processed clean, preferably with the weeds evulsion, tea tree in the ranks, coffee tree in the ranks or woodland open a wide 35cm, the ditch of dark 15cm.Next is that the bacterium bag is processed and put, the sack of bacterium bag is scratched with pocket knife, whole polyethylene (polypropylene) plastic sack is torn off, pollute if bacterium is surrounded by part, pollute part with hilt and remove, in the situation with good conditionsi, soak gdna contamination with limewash again, be horizontally placed on ditch after taking off bag, between the bacterium bag about spacing 2-3cm, after all the bacterium bag is put fully, fully pour water once.To cover the scholar at last, treat that moisture all floods down between ditch after, use the fine earth earthing, fill up slit and bacterium bag surface, the thick 2-3cm of earthing, and water sufficient water at earthing.
4. management of producing mushroom
After treating that the first damp mushroom fruiting fully, stop water spray, bacteria 8-10 days, then fully pouring water once went out the second damp mushroom.
Claims (8)
1. a strain schizophyllum commune high yield fruit body bacterial strain and return ecological stereo cultivation method, it adopts following steps:
Step 1, bacterial strain collection, domestication: by the collection to Yunnan Province's different hosts, the wild schizophyllum commune resource in habitat, utilize the introduction and acclimatization measures such as the separation of mushroom wood, fruit body inoculation, preserve the schizophyllum commune bacterial classification.Then by fruiting experiment, primary dcreening operation produces the stronger bacterial strain of fruit body ability;
Step 2, bacterial strain screening: with the bacterial strain behind the primary dcreening operation, the anti-assorted test such as, mould mould by wood is multiple sieve further, filters out by fruiting experiment that anti-hybrid ability is strong, the bacterial strain of stable yield at last again, and the bacterial strain that finishing screen is chosen is the GGHN08-26 bacterial strain:
Step 3, cultivation season: this bacterial strain all can return ecological cultivation throughout the year in Yunnan Province, but best cultivation season is early spring to autumn Mo;
Step 4, bacterium pack work: take cotton seed hulls, wood chip or bamboo bits, wheat bran, corn flour etc. as raw material, filling a prescription is cotton seed hulls 45%, wood chip/bamboo bits 30%, wheat bran 15%, corn flour 8%, lime 1%, sugar 1%.Water content is controlled between the 55%-65%, is made into 17 * 33cm bacterium bag, autoclaving 2 hours or normal-pressure sterilization 10 hours;
Step 5, inoculation: when the bacterium bag of the good bacterium of fire is cooled to begin about 25 ℃ inoculation, during inoculation, in the punching inoculation of bag body side, make a call to 3 inoculation holes, adhesive plaster seals behind the access bacterial classification;
Step 6, bacterium bag are cultivated: after the inoculation, the bacterium bag is put on culturing room's bedstead or the level land stacks a bacterium, sends out the bacterium culture environment and requires thermophilic, lucifuge, ventilation, drying.Keep 24-26 ℃ of room temperature, be beneficial to bacterial classification and sprout field planting and mycelial growth;
Step 7, slaking are cultivated: after mycelia is covered with in bag, still must continue to cultivate a period of time, make mycelia reach physiological ripening.Ripe standard is to form block hypohostroma at the bag wall, and pressing cultivation bag with hand has depression to occur.The time that slaking is cultivated is 10-15d approximately, and interior nitrogenous source nutrition and the nutrition of quick-acting carbon source of composts or fertilisers of cultivating this moment exhausts substantially, and changing also appears in the contents level of some organized enzyme;
Step 8, return ecological vertical cultivation: the arrangement groove face, tea weeds are in the ranks processed clean, preferably with the weeds evulsion; Trench digging, tea tree in the ranks, coffee tree in the ranks or woodland open a wide 35cm, the ditch of dark 15cm; The bacterium bag is processed, and the sack of bacterium bag is scratched with pocket knife, and whole polyethylene (polypropylene) plastic sack is torn off, pollutes if bacterium is surrounded by part, pollutes part with hilt and removes, and in the situation with good conditionsi, soaks gdna contamination with limewash again; The bacterium bag is put, and bacterium is horizontally placed on ditch after wrapping in and taking off bag, about spacing 2-3cm, after all the bacterium bag is put fully, fully pours water once between the bacterium bag; Earthing, treat that moisture all floods down between ditch after, use the fine earth earthing, fill up slit and bacterium bag surface, the thick 2-3cm of earthing, and water sufficient water at earthing; Management of producing mushroom, standard of plucking are that the schizophyllum commune cap is not carried out fully, and be best when color is milky, during harvesting, holds the stem base portion, and whole clump is taken.Adopted the later water spray of mushroom once every day during fruiting, and water spray is to be advisable on the moistening table.After treating that the first damp mushroom fruiting fully, stop water spray.Bacteria 8-10 days, then fully pouring water once went out the second damp mushroom.
2. described according to claim 1, schizophyllum commune GGHN08-26 bacterial strain is that a strain produces the stronger bacterial strain of fruit body ability.
3. described according to claim 1, schizophyllum commune GGHN08-26 bacterial strain is the stronger bacterial strain of a strain anti-hybrid ability.
4. described according to claim 1, the best cultivation season of this bacterial strain is early spring to autumn Mo.
5. described according to claim 1, this bacterial strain optimum feed stock is cotton seed hulls, wood chip or bamboo bits, wheat bran, corn flour, fills a prescription to be cotton seed hulls 45%, wood chip/bamboo bits 30%, wheat bran 15%, corn flour 8%, lime 1%, sugar 1%.Water content is controlled between the 55%-65%.
6. described according to claim 1, bacterium wraps in and requires thermophilic, lucifuge, ventilation, drying in the incubation.Keep about 25 ℃ of room temperatures.
7. described according to claim 1, after mycelia is covered with in bag, still must continue to cultivate a period of time, make mycelia reach physiological ripening.Ripe standard is to form block hypohostroma at the bag wall, and pressing cultivation bag with hand has depression to occur.
8. described according to claim 1, return ecologically to the tea place, when coffee garden, forest land cultivation, should process according to arrangement groove face, trench digging, bacterium bag, the steps such as the bacterium bag be put, earthing, in good time harvesting carry out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100604055A CN103299822A (en) | 2012-03-09 | 2012-03-09 | Schizophyllum commune Fr high-yield fruiting body strain and three-dimensional ecology-returning cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100604055A CN103299822A (en) | 2012-03-09 | 2012-03-09 | Schizophyllum commune Fr high-yield fruiting body strain and three-dimensional ecology-returning cultivation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103299822A true CN103299822A (en) | 2013-09-18 |
Family
ID=49125890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100604055A Pending CN103299822A (en) | 2012-03-09 | 2012-03-09 | Schizophyllum commune Fr high-yield fruiting body strain and three-dimensional ecology-returning cultivation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103299822A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521549A (en) * | 2014-11-29 | 2015-04-22 | 董淑英 | Artificial cultivation method for Tai-mushroom and Pan-mushroom |
| CN105567571A (en) * | 2015-12-23 | 2016-05-11 | 文山学院 | Method for isolating strains of air-dried fruiting bodies of schizophyllum commune |
| CN107828664A (en) * | 2017-10-25 | 2018-03-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT 1 and its cultural method and application |
| CN109220550A (en) * | 2018-10-31 | 2019-01-18 | 芒市喜丰农业发展有限公司 | A kind of method that white ginseng culture material waste residue recycles |
| CN109247191A (en) * | 2018-09-29 | 2019-01-22 | 云南菌视界生物科技有限公司 | A kind of cultural method of coralliform schizophyllum commune bacterial strain and its fructification |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1306084A (en) * | 2000-01-18 | 2001-08-01 | 罗星野 | Artificially cultured schizophyllum sporophores and its culture process |
| CN101643759A (en) * | 2009-07-31 | 2010-02-10 | 中国农业科学院农产品加工研究所 | Method for preparing Schizophyllum commune polysaccharide and special culture medium |
| CN101785393A (en) * | 2010-02-10 | 2010-07-28 | 云南农业大学 | Method for tea garden and edible fungi complex ecological production system |
-
2012
- 2012-03-09 CN CN2012100604055A patent/CN103299822A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1306084A (en) * | 2000-01-18 | 2001-08-01 | 罗星野 | Artificially cultured schizophyllum sporophores and its culture process |
| CN101643759A (en) * | 2009-07-31 | 2010-02-10 | 中国农业科学院农产品加工研究所 | Method for preparing Schizophyllum commune polysaccharide and special culture medium |
| CN101785393A (en) * | 2010-02-10 | 2010-07-28 | 云南农业大学 | Method for tea garden and edible fungi complex ecological production system |
Non-Patent Citations (2)
| Title |
|---|
| 李梦杰等: "云南裂褶菌宿主多样性及生境调查", 《云南农业大学学报》 * |
| 王翠玲等: "三株裂褶菌菌丝体营养条件的筛选", 《食用菌学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521549A (en) * | 2014-11-29 | 2015-04-22 | 董淑英 | Artificial cultivation method for Tai-mushroom and Pan-mushroom |
| CN105567571A (en) * | 2015-12-23 | 2016-05-11 | 文山学院 | Method for isolating strains of air-dried fruiting bodies of schizophyllum commune |
| CN107828664A (en) * | 2017-10-25 | 2018-03-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT 1 and its cultural method and application |
| CN107828664B (en) * | 2017-10-25 | 2020-10-23 | 湖南省农业生物技术研究中心 | Schizophyllum commune XT-1 and cultivation method and application thereof |
| CN109247191A (en) * | 2018-09-29 | 2019-01-22 | 云南菌视界生物科技有限公司 | A kind of cultural method of coralliform schizophyllum commune bacterial strain and its fructification |
| CN109220550A (en) * | 2018-10-31 | 2019-01-18 | 芒市喜丰农业发展有限公司 | A kind of method that white ginseng culture material waste residue recycles |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104041330B (en) | Ganoderma tsugae imitates wild juggle cultivation method | |
| CN103918475B (en) | The elegant precious method of mushroom bonsai type cultivation and the medium for cultivating elegant precious mushroom | |
| CN102630481B (en) | Cultivation method for oospore oudemansiella mucida | |
| CN103891524B (en) | The method of glossy ganoderma dish garden formula cultivation and the medium for cultivating ganoderma | |
| CN104193462A (en) | Agaric culture medium | |
| CN101766094A (en) | Black fungus and cultural method and application thereof | |
| CN103340093A (en) | Artificial cultivation method for phellinus linteus | |
| CN109479616B (en) | Seed production and cultivation method of tricholoma matsutake | |
| CN102742453A (en) | Novel tuckahoe strain and efficient cultivation technology thereof | |
| CN103907478A (en) | Method and medium for cultivating oyster mushrooms in potted landscape manner | |
| CN106431668A (en) | Black fungus cultivation material | |
| CN106495831A (en) | A kind of cultivation of auricularia auricula material | |
| CN101461312B (en) | Thousand-fold Subversion Method of the Mother Species of Shimejime | |
| CN103299822A (en) | Schizophyllum commune Fr high-yield fruiting body strain and three-dimensional ecology-returning cultivation method | |
| CN112442449B (en) | Ramaria original strain culture medium and application thereof as well as Ramaria original strain and culture method thereof | |
| CN112703963A (en) | Method for cultivating oyster mushroom by using stevia rebaudiana leaf residues | |
| CN107810782A (en) | A kind of high-yield method of bracteal leaf of corn cultivating ganoderma | |
| CN103980060A (en) | Chinese amanita fuliginea culture medium as well as preparation method and application thereof | |
| CN103113148A (en) | Culture medium for germinating boletus spore | |
| CN103396218A (en) | Formula of Pleurotus citrinopileatus stock compost and preparation method of compost | |
| CN111788990A (en) | White skin termitomyces albuminosus culture method | |
| CN114149927B (en) | Pleurotus geesteranus strain Qian Xiu No. 3 and application thereof | |
| KR101944613B1 (en) | Medium composite of shiitake and cultivation method using thereof | |
| CN109006163A (en) | A kind of Ganoderma tsugae cultivar cultural method, culture medium and culture medium preparation method | |
| CN104541965A (en) | Industrialized cultivation method of lyophyllum umbrella |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130918 |