CN106479903B - A kind of production method of living body glossy ganoderma dish garden - Google Patents

A kind of production method of living body glossy ganoderma dish garden Download PDF

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CN106479903B
CN106479903B CN201610900613.XA CN201610900613A CN106479903B CN 106479903 B CN106479903 B CN 106479903B CN 201610900613 A CN201610900613 A CN 201610900613A CN 106479903 B CN106479903 B CN 106479903B
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parts
culture
culture medium
cultivar
cultivation
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CN106479903A (en
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马传贵
张志秀
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Beijing Jingcheng Biology Technology Co Ltd
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Beijing Jingcheng Biology Technology Co Ltd
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Priority to CN201910936460.8A priority Critical patent/CN110476709A/en
Priority to CN201910937295.8A priority patent/CN110521489A/en
Priority to CN201910936458.0A priority patent/CN110476708A/en
Priority to CN201910936457.6A priority patent/CN110476707A/en
Priority to CN201610900613.XA priority patent/CN106479903B/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/40Cultivation of spawn
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The present invention relates to cultivation of glossy ganoderma technical fields, more particularly to a kind of production method of living body glossy ganoderma dish garden, including obtaining lucid ganoderma stock culture, ganoderma lucidum original seed, cultivation of glossy ganoderma kind respectively, carry out substituting stuff cultivation, sesame out, transplanting periodically or non-periodically inputs nutrient solution and/or water into bonsai type container into the bonsai type container;The culture medium of substituting stuff cultivation is identical as Cultivar culture medium;Wherein Cultivar culture medium includes sawdust, pig manure, cow dung, rapeseed dregs, humic acid, Mycen osmundicola fermentation liquid, bacillus subtilis fermentation liquor, gypsum.The present invention is remarkably improved ganoderma lucidum fruitbody yield, and also significantly extends the storage life of living body glossy ganoderma dish garden and improve its preservation quality.

Description

A kind of production method of living body glossy ganoderma dish garden
Technical field
The present invention relates to cultivation of glossy ganoderma technical fields, and in particular to a kind of production method of living body glossy ganoderma dish garden.
Background technique
Ganoderma lucidum (Ganoderma Lucidum Karst) is the fructification of On Polyporaceae ganoderma lucidum.With invigorating qi for tranquilization, Relieving cough and asthma, the effect of promoting longevity.For dizziness egersis, shortness of breath and palpitation, neurasthenia, consumptive disease cough and asthma.Ganoderma lucidum is China doctor One of treasure-house rare Chinese medicine is learned, is to integrate health care and medicinal as the edible mushroom of the whole body.Modern medicine study proves, in ganoderma lucidum Polysaccharide, triterpenes, sterols isoreactivity ingredient have antitumor, adjusting to be immunized human body, adjust nervous system, adjust circulation The function of system etc..Ganoderma lucidum, it is mild-natured, for strengthening by means of tonics, the precious tonic for consolidating righting, promoting longevity.The ancient medicine in China Record ganoderma lucidum in the Compendium of Material Medica of treasured book Shennong's Herbal and Li Shizhen (1518-1593 A.D.): " upper medicine-feeding, main life of supporting is with Ying Tian.Invigorating heart gas is mended In, increase intelligence, hard muscles and bones, good color, food is made light of one's life by commiting suicide long." ganoderma lucidum implied meaning is beautiful, lucky, rich and honour, long-lived, as one wishes.Sheng Nong's herbal classic It records, " essence of mountains and rivers sexual intercourse five-element YIN YANG in four seasons round the clock, can give birth to multicolored ganoderma lucidum, and all holy king stops auspicious easy ";Solid " the spirit of Han dynasty class Sesame song " is praised: " as three morals Ying Ruitu, life of lengthening the life and light longevity are all ";Wei is towards the road Cao Zhi " ganoderma lucidum forever " Shi Zhongyezan: " high honour phase Credit is shaken, if honorable bright refreshing ".
Glossy ganoderma dish garden scene is beautiful, simple and unsophisticated pure and fresh, and implied meaning is lucky rich and honour, has deep artistic connotation, has become height The first choice of shelves decoration, and the fashion good merchantable brand of present, collection.Producing glossy ganoderma dish garden has high economic benefit and society's effect Benefit.
Summary of the invention
It is an object of the present invention to provide a kind of production methods of living body glossy ganoderma dish garden.
Technical solution of the present invention is as follows:
A kind of production method of living body glossy ganoderma dish garden, comprising the following steps:
1) lucid ganoderma stock culture is seeded to mother culture media, obtains lucid ganoderma stock culture;
The mother culture media includes following component: potato 200-300g/L, glucose 20-30g/L, beef extract 3- 5g/L, peptone 4-10g/L, agar powder 15-20g/L;
2) lucid ganoderma stock culture is seeded to pedigree seed culture medium culture, obtains ganoderma lucidum original seed;
The pedigree seed culture medium includes the ingredient of following parts by weight: 60-80 parts of sawdust, 5-10 parts of corncob, wheat bran 10-15 Part, 1-3 parts of brown sugar, 1-3 parts of land plaster, KNO30.05-0.5 parts;The pedigree seed culture medium water content is 55-60%;
3) ganoderma lucidum original seed is seeded in Cultivar culture medium and is cultivated, obtain cultivation of glossy ganoderma kind;
The Cultivar culture medium includes the raw material of following parts by weight: 30-80 parts of sawdust, 5-15 parts of pig manure, cow dung 5-15, 5-25 parts of rapeseed dregs, 5-10 parts of humic acid, Mycen osmundicola (Mycena osmundicola Lange) 1-10 parts of fermentation liquid, withered grass 1-10 parts, 1-3 parts of gypsum of bacillus (Bacillus subtilis) fermentation liquid;It is effectively living in the Mycen osmundicola fermentation liquid Bacterium number is 1.0-5.0 × 106/ mL, living bacteria count is 1.0-1.5 × 10 in the bacillus subtilis fermentation liquor10/mL;Institute The preparation method of Cultivar culture medium is stated the following steps are included: according to the ratio mixing each raw material, adds water to water content 50-70%, It ferments to decomposed;Moisture content is adjusted to 55-60%, sterilizing;
4) the cultivation of glossy ganoderma kind is subjected to substituting stuff cultivation, out sesame;The culture medium of the substituting stuff cultivation and cultivar culture Base phase is same;
5) ganoderma lucidum obtained by step 4) is transplanted into bonsai type container, it is periodically or non-periodically defeated into the bonsai type container Enter nutrient solution and/or water;
Matrix in the bonsai type container includes the raw material of following parts by weight: 80-100 parts of sandy loam, sawdust 10-30 Part, 1-3 parts of plant ash, 1-3 parts of land plaster;
The bonsai type container and the matrix is sterilized in advance or disinfection treatment, completely removes microorganism and moth or worm Ovum;
Nutrient solution described in every 1kg includes following component: Cultivar culture medium leachate 50-100mL, dehydroactic acid sodium 500- 1000mg, MgSO4100-500mg, KNO3100-200mg, K2HPO450-100mg, Triiodobenzoic acid 100-200mg, EDTA-2Na (i.e. disodium ethylene diamine tetraacetate) 5-10mg, vitamin B15-10mg, water (solvent);
It includes the water that the Cultivar culture medium is added to 10 times of weight that the Cultivar culture medium, which leaches liquid and preparation method thereof, 10-24h is impregnated, filtering sterilizes filtrate.
Preferably, the mother culture media includes following component: potato 250g/L, glucose 25g/L, beef extract 3g/ L, peptone 6g/L, agar powder 15-20g/L.
Preferably, the Mother culture condition include: at 22-26 DEG C constant temperature be protected from light culture;General culture 5-7 days Mycelia covers with medium slant.
Preferably, the pedigree seed culture medium includes the ingredient of following parts by weight: 70 parts of sawdust, 6 parts of corncob, wheat bran 15 Part, 2 parts of brown sugar, 2 parts of land plaster, KNO30.25 part;The pedigree seed culture medium water content is 55-60%.
Preferably, the Primary spawn condition include: in the case where temperature is 20-22 DEG C, humidity is 55-65% constant temperature be protected from light training It supports, general culture 35-40 days.
Preferably, the Cultivar culture medium or for material culture medium, the raw material including following parts by weight: 40 parts of sawdust, pig 5 parts of excrement, 15 parts of cow dung, 15 parts of rapeseed dregs, 6 parts of humic acid, 5 parts of Mycen osmundicola fermentation liquid, 5 parts of bacillus subtilis fermentation liquor, 2 parts of gypsum;Living bacteria count is 1.0-3.0 × 10 in the Mycen osmundicola fermentation liquid6/ mL, the fermentation of bacillus subtilis Living bacteria count is 1.0-1.2 × 10 in liquid10/mL。
Preferably, the cultivar condition of culture includes: the constant temperature training in the case where temperature is 20-22 DEG C, humidity is 55-65% It supports, general culture 35-50 days;Culture is protected from light in 10 days early periods, later period intensity of illumination is 1000-1500 1x (lux);Favorably In mycelia fast-growth.
Mother culture media of the present invention, pedigree seed culture medium can be made by conventional method in that art.
The substituting stuff cultivation goes out sesame incubation time and is generally 30-50d.
Preferably, it is 28-30 DEG C that the substituting stuff cultivation condition (going out sesame condition), which includes: cultivation temperature, and air is relatively wet Degree is 60-75%, and culture is protected from light in 10 days early periods, and later period intensity of illumination is 1000-1500 1x (lux);Be conducive to mycelia Fast-growth.
Preferably, the sesame out is to finalize sesame, including artificial position control goes out sesame or manually finalizes sesame, can be by normal Rule method carries out.
Preferably, retain part substituting stuff cultivation culture medium when step 5) transplanting ganoderma lucidum, being subject to ensures ganoderma lucidum transplant survival.
Preferably, the matrix in the bonsai type container includes the raw material of following parts by weight: 85 parts of sandy loam, sawdust 12 Part, 2 parts of plant ash, 2 parts of land plaster;
Preferably, nutrient solution described in every 1kg includes following component: Cultivar culture medium leachate 80mL, dehydroactic acid sodium 600mg, MgSO4300mg, KNO3150mg, K2HPO4100mg, Triiodobenzoic acid 180mg, EDTA-2Na 10mg, vitamin B110mg, water (solvent);
The nutrient solution includes according to a conventional method mixing each raw material in proportion.Preferably, by the nutrient solution into Row sterilization treatment, it is putrid and deteriorated to prevent.
The present invention also provides the matrix in a kind of bonsai type container for the production of living body glossy ganoderma dish garden, the bonsai type holds Matrix in device includes the raw material of following parts by weight: 80-100 parts of sandy loam, 10-30 parts of sawdust, and 1-3 parts of plant ash, land plaster 1-3 parts;Preferably, the matrix in the bonsai type container includes the raw material of following parts by weight: 85 parts of sandy loam, 12 parts of sawdust, 2 parts of plant ash, 2 parts of land plaster.
The general sandy loam of sandy loam of the present invention.
The nutrient solution that the present invention also provides a kind of for the production of living body glossy ganoderma dish garden (is mainly used for inputting in bonsai type container Matrix in), nutrient solution described in every 1kg includes following component: Cultivar culture medium leachate 50-100mL, dehydroactic acid sodium 500-1000mg, MgSO4100-500mg, KNO3100-200mg, K2HPO450-100mg, Triiodobenzoic acid 100-200mg, EDTA-2Na5-10mg, vitamin B15-10mg, water (solvent);Preferably, nutrient solution described in every 1kg includes following component: being planted Cultivate culture medium leachate 80mL, dehydroactic acid sodium 600mg, MgSO4300mg, KNO3150mg, K2HPO4100mg, triiodo-benzene Formic acid 180mg, EDTA-2Na 10mg, vitamin B110mg, water (solvent).
The present invention also provides a kind of Cultivar culture medium of cultivating ganoderma or for material culture medium, the original including following parts by weight Material: 30-50 parts of sawdust, 5-15 parts of pig manure, 5-15 parts of cow dung, 5-25 parts of rapeseed dregs, 5-10 parts of humic acid, Mycen osmundicola 1-10 parts of (Mycenaosmundicola Lange) zymotic fluid, bacillus subtilis (Bacillus subtilis) fermentation liquid 1-10 Part, 1-3 parts of gypsum;Living bacteria count is 1.0-5.0 × 10 in the Mycen osmundicola fermentation liquid6/ mL, the bacillus subtilis Living bacteria count is 1.0-1.5 × 10 in fermentation liquid10/mL;The preparation method of the Cultivar culture medium is the following steps are included: press Proportion mixes each raw material, adds water to water content 50-70%, ferments to decomposed;Moisture content is adjusted to 55-60%, is sterilized, i.e., It can.
Preferably, the Cultivar culture medium or for material culture medium, the raw material including following parts by weight: 40 parts of sawdust, pig 5 parts of excrement, 15 parts of cow dung, 15 parts of rapeseed dregs, 6 parts of humic acid, 5 parts of Mycen osmundicola fermentation liquid, 5 parts of bacillus subtilis fermentation liquor, 2 parts of gypsum;Living bacteria count is 1.0-3.0 × 10 in the Mycen osmundicola fermentation liquid6/ mL, the fermentation of bacillus subtilis Living bacteria count is 1.0-1.2 × 10 in liquid10/mL。
The humic acid is commercially available to be bought, such as purchased from Shandong Creation Hhumic Acid Science and Technology Co., Ltd.
The bacillus subtilis is preferably CGMCC 1.3358.
Mycen osmundicola of the present invention, bacillus subtilis can be by commercially available, such as purchased from Chinese common micro- Biological inoculum collection.
Cultivar culture medium needs thoroughly inactivation bacillus subtilis for sterilizing described in the preparation method for expecting culture medium And its various microorganisms such as gemma, this field conventional sterilization procedures can be used.
Conventional method in that art can be used in the Mycen osmundicola fermentation liquid preparation, such as preparation method includes taking Mycen osmundicola Parent species, are inoculated in culture solution, and shaking table culture 5-10d filters Mycen osmundicola bacterium ball with gauze to get Mycen osmundicola fermentation liquid;It can It is placed in refrigerator and saves.The preferred 140-160rpm shaken cultivation of shaking table culture revolving speed, preferred 22-26 DEG C of cultivation temperature;One As the bottled Culture liquid measure 125-150mL of 250mL triangle, the bottled Culture liquid measure 250-300mL of 500mL triangle.
Conventional method in that art can be used in the bacillus subtilis fermentation liquor preparation, for example including following steps:
1) inclined-plane culture: the original strain of bacillus subtilis is aseptically inoculated on slant medium, 36-48h is cultivated under the conditions of 29 ± 1 DEG C;The formula of slant medium is as follows: glucose 15g, peptone 5g, yeast extract 5g, water 1000mL, agar 15g;
2) shaking table culture: the strain that step 1) culture obtains is inoculated in fluid nutrient medium, is in pH 6.5-7.0, temperature Under the conditions of 30 ± 2 DEG C, 140-160r/min shaking table culture 36-48h;Liquid Culture based formulas is as follows: corn flour 13g, glucose 5g, beancake powder 20g, fish meal 5g, CaCO32g, (NH4)2SO41g, K2HPO40.3g, MgSO4·7H2O 0.2g, MnSO4· H2O0.2g, water 1000ml;
3) fermentation tank culture: the strain that step 2) culture obtains is inoculated in fermentation tank culture medium, in pH 7.5-8.0, tank Pressing 0.5kg, temperature is 30 ± 2 DEG C, under the conditions of ventilation quantity 1:0.8-1.1, until bacterium number is greater than 1.0-1.2 × 1010/ mL tank at present, Up to bacillus subtilis fermentation liquor;Fermentation tank culture based formulas is as follows: corn flour 5kg, beancake powder 2.5kg, ammonium sulfate 0.5kg, glucose 1.5kg, yeast powder 0.5kg, peptone 0.25kg, 100kg is added water to;It is generally incubated 48-56h.
Specifically, Cultivar culture medium or for material culture medium preparation method described in fermentation to it is decomposed include: fermentation heap First time turning when interior temperature reaches 50 DEG C, turning daily 1-2 times later, and amount of water is supplemented, it is reduced to fermentation heap temperature 30% or less end fermentation is down to 35 DEG C or less, moisture content.
The method of the present invention is suitable for various ganoderma lucidums, is particularly suitable for ganoderma lucidum Ganoderma lucidum (Leyss.:Fr.) Karst;Purple sesame Ganoderma sinense J.D.Zhao, L.W.Hsu et X.Q.Zhang;Ganoderma capuse Ganoderma capense(L loyd)D.A.Reid。
Lucidum strain of the present invention is commercially available to be bought, can also be isolated from Wild ganoderma fructification.
Present invention optimizes mother culture media, pedigree seed culture medium, Cultivar culture mediums, make Mycelium Growth of Ganoderma lucidum faster, more Be conducive to later period formation fructification.Particularly, Cultivar culture medium of the present invention is remarkably improved ganoderma lucidum fruitbody yield.The present invention Mycen osmundicola is applied in the fermentation of cultivation of glossy ganoderma kind culture medium compost for the first time, obtained Cultivar culture medium (also is used as Generation material culture medium) have bacteria resistance function, bacterial contamination rate is significantly reduced, ganoderma lucidum quality is improved, increases economic benefit.This Inventive method is simple and easy to do, and investment is small, quick, easy to spread;The method of the present invention mycelial growth rate is fast, and mycelia daily grows Speed is up to 18-20mm/d, and for fructification biological efficiency up to 9%-15%, fruiting body yield is high, high-quality, significantly improves Economic benefit.The present invention also optimizes bonsai type container matrix and nutrient solution.The method of the present invention significantly extends living body ganoderma lucidum The storage life of potted landscape and improve its preservation quality.The glossy ganoderma dish garden of the method for the present invention production is living body ganoderma lucidum, experiments have shown that Ganoderma lucidum still keeps condition of living organism after storage 3 years, and the glossy ganoderma dish garden for the phenomenon that occurs damaging by worms in 3 years is less than 10%.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..It is not specified in embodiment specific Technology or conditions person, described technology or conditions according to the literature in the art, or carried out according to product description.It is used Production firm person is not specified in reagent or instrument, is the conventional products that can be commercially available by regular distributor.
Strain of the present invention can be by commercially available.Bacillus subtilis as described below is CGMCC 1.3358.
Living body ganoderma lucidum experimental method includes: that will be cut in the ganoderma lucidum fruitbody in glossy ganoderma dish garden with sterile scalpel, with solution Cut open cutter cuts 0.2-0.5cm small cube tissue, and the tissue is placed in culture dish PDA culture medium (including following component: horse with tweezers Bell potato 200g/L, glucose 20g/L, agar powder 15g/L) center, cultivate 2-3d after it is visible white hypha is grown by tissue block, Prove living body ganoderma lucidum.Continue the tube 1-2 times Ganoderma lucidum mycelium that can get purifying, it is real still to can get ganoderma lucidum by the method for the present invention Body.
Embodiment 1
A kind of production method of living body glossy ganoderma dish garden, comprising the following steps:
1) ganoderma lucidum Ganoderma lucidum (Leyss.:Fr.) Karst parent species are seeded to mother culture media in 22-26 Constant temperature incubation 5-7 days at DEG C obtain lucid ganoderma stock culture.The mother culture media includes following component: potato 250g/L, glucose 25g/L, beef extract 3g/L, peptone 6g/L, agar powder 15-20g/L.
2) lucid ganoderma stock culture is seeded to pedigree seed culture medium at 20-22 DEG C of temperature, humidity 55-65% to cultivate, obtains ganoderma lucidum Original seed.The pedigree seed culture medium includes the ingredient of following parts by weight: 70 parts of sawdust, 6 parts of corncob, 15 parts of wheat bran, 2 parts of brown sugar, 2 parts of land plaster, KNO30.25 part;The pedigree seed culture medium water content is 55-60%;500mL original seed is made in pedigree seed culture medium Bottle.The preparation method of the pedigree seed culture medium includes taking each raw material according to the ratio, and moisture content is adjusted after mixing, is sterilized.
The Mother culture, Primary spawn condition are protected from light culture in darkroom.
3) ganoderma lucidum original seed is seeded in the Cultivar culture medium in cultivating bag and is cultivated, obtain cultivation of glossy ganoderma kind.The cultivation It cultivates condition of culture and is included in that temperature is 20-22 DEG C, humidity is constant temperature incubation under 55-65%, general culture 35-50 days;Early period Culture is protected from light in 10 days, later period intensity of illumination is 1000-1500 1x (lux).
The Cultivar culture medium includes the raw material of following parts by weight: 40 parts of sawdust, 5 parts of pig manure, 15 parts of cow dung, rapeseed dregs 15 parts, 6 parts of humic acid, 5 parts of Mycen osmundicola fermentation liquid, 5 parts of bacillus subtilis fermentation liquor, 2 parts of gypsum;The Mycen osmundicola hair Living bacteria count is 1.0-3.0 × 10 in zymotic fluid6/ mL, living bacteria count is 1.0-1.2 in the bacillus subtilis fermentation liquor ×1010/mL.The Mycen osmundicola zymotic fluid preparation method includes taking Mycen osmundicola parent species, is inoculated in culture solution, shaking table 140rpm 7-8d is cultivated, 22-26 DEG C of cultivation temperature, filters Mycen osmundicola bacterium ball with gauze to get Mycen osmundicola fermentation liquid.
The preparation method of the Cultivar culture medium adds water to aqueous the following steps are included: according to the ratio mix each raw material 50-70%, first time turning when temperature reaches 50 DEG C in fermentation heap, later turning daily 1-2 times are measured, and supplements amount of water, To fermentation heap temperature be reduced to 35 DEG C or less, moisture content be down to 30% or less and show fermentation maturity, terminate fermentation;Adjust moisture Content is to 55-60%, and thoroughly sterilize (i.e. the thoroughly inactivation various microorganisms such as bacillus subtilis and its gemma).
4) cultivation of glossy ganoderma kind progress substituting stuff cultivation is gone out into sesame culture, carries out artificial position control according to a conventional method and goes out sesame Or sesame is manually finalized, obtain ganoderma lucidum.The generation material culture medium is identical as Cultivar culture medium.
The substituting stuff cultivation condition includes: that cultivation temperature is 28-30 DEG C, relative air humidity 60-75%, 10 days early periods It is inside protected from light culture, later period intensity of illumination is 1000-1500 1x (lux).
5) ganoderma lucidum obtained by step 4) is transplanted into bonsai type container, it is periodically or non-periodically defeated into the bonsai type container Enter nutrient solution and/or water;Retain part substituting stuff cultivation culture medium when transplanting ganoderma lucidum, being subject to ensures ganoderma lucidum transplant survival.
Matrix in the bonsai type container includes the raw material of following parts by weight: 85 parts of sandy loam, 12 parts of sawdust, and plant ash 2 parts, 2 parts of land plaster;
The bonsai type container and the matrix is sterilized in advance or disinfection treatment, completely removes microorganism and moth or worm Ovum;
Nutrient solution described in every 1kg includes following component: Cultivar culture medium leachate 80mL, dehydroactic acid sodium 600mg, MgSO4300mg, KNO3150mg, K2HPO4100mg, Triiodobenzoic acid 180mg, EDTA-2Na 10mg, vitamin B110mg, Surplus is water (solvent).
It includes the water that the Cultivar culture medium is added to 10 times of weight that the Cultivar culture medium, which leaches liquid and preparation method thereof, It impregnates for 24 hours, filtering sterilizes filtrate.
The nutrient solution includes according to a conventional method mixing each raw material in proportion, sterilizing.
Parent species, original seed, cultivar, the average daily speed of growth of cultivating bag mycelia are up to 18-20mm/d.From former base to fructification Maturation generally needs 30-45d, can harvest within 1.5-2 months after general culture;In terms of the dry generation material dry weight of 1000kg, it can adopt for the year Receive ganoderma lucidum fruitbody 90-125kg.(for fruiting body yield in terms of dry weight, the yield calculated herein only refers to step 4 gained ganoderma lucidum yield, Similarly hereinafter.) the present embodiment production glossy ganoderma dish garden be living body ganoderma lucidum, experiments have shown that through storage 3 years after ganoderma lucidum still keep condition of living organism; Occur damaging by worms in 3 years phenomenon glossy ganoderma dish garden less than 10%.
Embodiment 2
A kind of production method of living body glossy ganoderma dish garden, the difference with embodiment 1 are only that: the mother culture media include with Lower ingredient: potato 200g/L, glucose 20g/L, beef extract 3g/L, peptone 4g/L, agar powder 15g/L;The original seed training Feeding base includes the ingredient of following parts by weight: 60 parts of sawdust, 5 parts of corncob, 10 parts of wheat bran, 1 part of brown sugar, 1 part of land plaster, KNO3 0.05 part;The pedigree seed culture medium water content is 55-60%;The Cultivar culture medium includes the raw material of following parts by weight: wood Consider 30 parts, 5 parts of pig manure, cow dung 10,5 parts of rapeseed dregs, 5 parts of humic acid, 1 part of Mycen osmundicola fermentation liquid, fermentation of bacillus subtilis to be worth doing 1 part of liquid, 1 part of gypsum;Living bacteria count is 1.0-2.0 × 10 in the Mycen osmundicola fermentation liquid6/ mL, the bacillus subtilis Living bacteria count is 1.0-1.2 × 10 in fermented liquid10/mL.Matrix in the bonsai type container includes following parts by weight Raw material: 80 parts of sandy loam, 10 parts of sawdust, 1 part of plant ash, 1 part of land plaster;Nutrient solution described in every 1kg includes following component: cultivation Kind culture medium leachate 50mL, dehydroactic acid sodium 500mg, MgSO4100mg, KNO3100mg, K2HPO450mg, triiodo-benzene first Sour 100mg, EDTA-2Na 5mg, vitamin B15mg, surplus are water (solvent).
Parent species, original seed, cultivar, the average daily speed of growth of cultivating bag mycelia are up to 18-20mm/d.From former base to fructification Maturation generally needs 30-45d, can harvest within 1.5-2 months after general culture;In terms of the dry generation material dry weight of 1000kg, it can adopt for the year Receive ganoderma lucidum fruitbody 90-120kg.The glossy ganoderma dish garden of the present embodiment production is living body ganoderma lucidum, experiments have shown that the ganoderma lucidum after storage 3 years Still keep condition of living organism.Occur damaging by worms in 3 years phenomenon glossy ganoderma dish garden less than 10%.
Embodiment 3
A kind of production method of living body glossy ganoderma dish garden, the difference with embodiment 1 are only that: the mother culture media include with Lower ingredient: potato 300g/L, glucose 30g/L, beef extract 5g/L, peptone 10g/L, agar powder 20g/L;The original seed training Feeding base includes the ingredient of following parts by weight: 80 parts of sawdust, 10 parts of corncob, 15 parts of wheat bran, 3 parts of brown sugar, 3 parts of land plaster, KNO3 0.3 part;The pedigree seed culture medium water content is 55-60%;The Cultivar culture medium includes the raw material of following parts by weight: sawdust 50 parts, 15 parts of pig manure, 5 parts of cow dung, 25 parts of rapeseed dregs, 10 parts of humic acid, 10 parts of Mycen osmundicola fermentation liquid, bacillus subtilis hair 10 parts of zymotic fluid, 3 parts of gypsum;Living bacteria count is 1.0-2.0 × 10 in the Mycen osmundicola fermentation liquid6/ mL, the withered grass gemma Living bacteria count is 1.0-1.2 × 10 in bacillus fermentation liquid10/mL.Matrix in the bonsai type container includes following parts by weight Raw material: 100 parts of sandy loam, 30 parts of sawdust, 3 parts of plant ash, 3 parts of land plaster;Nutrient solution described in every 1kg includes following component: Cultivar culture medium leachate 100mL, dehydroactic acid sodium 1000mg, MgSO4500mg, KNO3200mg, K2HPO4100mg, three Iodo-benzoic acid 200mg, EDTA-2Na 10mg, vitamin B110mg, surplus are water (solvent).
Parent species, original seed, cultivar, the average daily speed of growth of cultivating bag mycelia are up to 18-20mm/d.From former base to fructification Maturation generally needs 30-45d, can harvest within 1.5-2 months after general culture;In terms of the dry generation material dry weight of 1000kg, it can adopt for the year Receive ganoderma lucidum fruitbody 90-120kg.The glossy ganoderma dish garden of the present embodiment production is living body ganoderma lucidum, experiments have shown that the ganoderma lucidum after storage 3 years Still keep condition of living organism.Occur damaging by worms in 3 years phenomenon glossy ganoderma dish garden less than 10%.
Comparative example 1
A kind of production method of living body glossy ganoderma dish garden, the difference with embodiment 1 are only that: mother culture media is trained using PDA Support base, including following component: potato 200g/L, glucose 20g/L, agar powder 15g/L;Pedigree seed culture medium includes following weight Part ingredient: 80 parts of hardwood sawdust, 18 parts of wheat bran, 1 part of sucrose, 1 part of land plaster, after mixing evenly plus water, final culture medium Water content is 55-60%;The Mother culture, Primary spawn are that 22 DEG C of -25 DEG C of constant temperature darkrooms are protected from light culture;The cultivar Culture medium is identical as substituting stuff cultivation culture medium, the ingredient including following parts by weight: 73 parts of weed tree sawdust, 25 parts of wheat bran, and soybean powder 1 Part, 1 part of land plaster.The substituting stuff cultivation condition includes: that cultivation temperature is 25-26 DEG C, relative air humidity 60-95%, preceding The lux 20d intensity of illumination 1000-1500, later period intensity of illumination 1500-3000 lux.The glossy ganoderma dish garden made is only regular Or water irregularly is inputted into the bonsai type container, but do not input any nutrient solution.
Parent species, original seed, cultivar, the general 10-15mm/d of the average daily speed of growth of cultivating bag mycelia.From former base to fructification at It is ripe generally to need 40-50d, it can harvest within 2-2.5 months after general culture;In terms of the dry generation material dry weight of 1000kg, it can harvest for the year Ganoderma lucidum fruitbody 40-50kg.The glossy ganoderma dish garden of phenomenon of occurring damaging by worms in 1 year is greater than 30%.
Comparative example 2
A kind of production method of living body glossy ganoderma dish garden, the difference with embodiment 1 are only that: mother culture media is trained using PDA Support base, including following component: potato 200g/L, glucose 20g/L, agar powder 15g/L;Pedigree seed culture medium includes following weight The ingredient of part: 80 parts of sawdust, 6 parts of corncob, 18 parts of wheat bran, 2 parts of land plaster;After mixing evenly plus water, final culture medium are aqueous Amount is 55-60%;The Mother culture, Primary spawn are that 22 DEG C of -25 DEG C of constant temperature darkrooms are protected from light culture.The cultivar culture Base is identical as substituting stuff cultivation culture medium, the ingredient including following parts by weight: 78 parts of cotton seed hulls, 20 parts of wheat bran, 1 part of sucrose, gypsum 1 Part;The substituting stuff cultivation condition includes: that cultivation temperature is 25-26 DEG C, relative air humidity 60-95%, preceding 20d intensity of illumination The lux 1000-1500, later period intensity of illumination 1500-3000 lux.The glossy ganoderma dish garden made only periodically or non-periodically to Water is inputted in the bonsai type container, but does not input any nutrient solution.
Parent species, original seed, cultivar, the general 10-15mm/d of the average daily speed of growth of cultivating bag mycelia.From former base to fructification at It is ripe generally to need 40-50d, it can harvest within 2-2.5 months after general culture;In terms of the dry generation material dry weight of 1000kg, it can harvest for the year Ganoderma lucidum fruitbody 40-50kg.The glossy ganoderma dish garden of phenomenon of occurring damaging by worms in 1 year is greater than 30%.
Comparative example 3
A kind of production method of living body glossy ganoderma dish garden, the difference with embodiment 1 are only that: mother culture media is trained using PDA Support base, including following component: potato 200g/L, glucose 20g/L, agar powder 15g/L;Pedigree seed culture medium includes following weight The ingredient of part: 80 parts of sawdust, 6 parts of corncob, 18 parts of wheat bran, 2 parts of land plaster;After mixing evenly plus water, final culture medium are aqueous Amount is 55-60%;The Mother culture, Primary spawn are that 22 DEG C of -25 DEG C of constant temperature darkrooms are protected from light culture.The cultivar culture Base is identical as substituting stuff cultivation culture medium, the ingredient including following parts by weight: 43 parts of cotton seed hulls, 42 parts of weed tree sawdust, 10 parts of wheat bran, beans 3 parts of cake powder, 1 part of calcium superphosphate, 1 part of gypsum.The substituting stuff cultivation condition includes: that cultivation temperature is 25-26 DEG C, and air is relatively wet Degree is 60-95%, the preceding lux 20d intensity of illumination 1000-1500, later period intensity of illumination 1500-3000 lux.It makes Glossy ganoderma dish garden only periodically or non-periodically inputs water into the bonsai type container, but does not input any nutrient solution.
Parent species, original seed, cultivar, the general 10-15mm/d of the average daily speed of growth of cultivating bag mycelia.From former base to fructification at It is ripe generally to need 40-50d, it can harvest within 2-2.5 months after general culture;In terms of the dry generation material dry weight of 1000kg, it can harvest for the year Ganoderma lucidum fruitbody 40-50kg.The glossy ganoderma dish garden of phenomenon of occurring damaging by worms in 1 year is greater than 30%.
Comparative example 4
A kind of production method of living body glossy ganoderma dish garden, the difference with embodiment 1 are only that: the Cultivar culture medium and generation The raw material for expecting culture medium does not include Mycen osmundicola fermentation liquid and bacillus subtilis fermentation liquor.
Parent species, original seed, cultivar, the general 13-16mm/d of the average daily speed of growth of cultivating bag mycelia.From former base to fructification at It is ripe generally to need 40-45d, it can harvest within 2-2.5 months after general culture;In terms of the dry generation material dry weight of 1000kg, it can harvest for the year Ganoderma lucidum fruitbody 50-60kg.The glossy ganoderma dish garden of phenomenon of occurring damaging by worms in 1 year is greater than 25%.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.

Claims (8)

1. a kind of production method of living body glossy ganoderma dish garden, which comprises the following steps:
1) lucid ganoderma stock culture is seeded to mother culture media, obtains lucid ganoderma stock culture;
The mother culture media includes following component: potato 200-300g/L, glucose 20-30g/L, beef extract 3-5g/L, Peptone 4-10g/L, agar powder 15-20g/L;
2) lucid ganoderma stock culture is seeded to pedigree seed culture medium culture, obtains ganoderma lucidum original seed;
The pedigree seed culture medium includes the ingredient of following parts by weight: 60-80 parts of sawdust, 5-10 parts of corncob, 10-15 parts of wheat bran, 1-3 parts of brown sugar, 1-3 parts of land plaster, KNO30.05-0.5 parts;The pedigree seed culture medium water content is 55-60%;
3) ganoderma lucidum original seed is seeded in Cultivar culture medium and is cultivated, obtain cultivation of glossy ganoderma kind;
The Cultivar culture medium includes the raw material of following parts by weight: 30-50 parts of sawdust, 5-15 parts of pig manure, 5-15 parts of cow dung, dish 5-25 parts of the seed dregs of rice, 5-10 parts of humic acid, 1-10 parts of Mycen osmundicola fermentation liquid, 1-10 parts of bacillus subtilis fermentation liquor, gypsum 1-3 Part;Living bacteria count is 1.0-5.0 × 10 in the Mycen osmundicola fermentation liquid6/ mL has in the bacillus subtilis fermentation liquor Effect viable count is 1.0-1.5 × 1010/mL;The preparation method of the Cultivar culture medium is the following steps are included: according to the ratio by each original Material mixing, adds water to water content 50-70%, ferments to decomposed;Moisture content is adjusted to 55-60%, sterilizing;
4) the cultivation of glossy ganoderma kind is subjected to substituting stuff cultivation, out sesame;The culture medium of the substituting stuff cultivation and Cultivar culture medium phase Together;
5) ganoderma lucidum obtained by step 4) is transplanted into bonsai type container, battalion is periodically or non-periodically inputted into the bonsai type container Nutrient solution and/or water;
Matrix in the bonsai type container includes the raw material of following parts by weight: 80-100 parts of sandy loam, 10-30 parts of sawdust, and grass 1-3 parts of wood ash, 1-3 parts of land plaster;
The bonsai type container and the matrix is sterilized in advance or disinfection treatment, completely removes microorganism and moth or worm's ovum;
Nutrient solution described in every 1kg includes following component: Cultivar culture medium leachate 50-100mL, dehydroactic acid sodium 500- 1000mg, MgSO4100-500mg, KNO3100-200mg, K2HPO450-100mg, Triiodobenzoic acid 100-200mg, EDTA-2Na5-10mg, vitamin B15-10mg, water;
It includes the water immersion that the Cultivar culture medium is added to 10 times of weight that the Cultivar culture medium, which leaches liquid and preparation method thereof, 10-24h, filtering, filtrate is sterilized.
2. manufacturing method according to claim 1, which is characterized in that the Mother culture condition includes: at 22-26 DEG C Constant temperature is protected from light culture.
3. production method according to claim 1 or 2, which is characterized in that the Primary spawn condition includes: to be in temperature 20-22 DEG C, humidity be 55-65% under constant temperature be protected from light culture.
4. production method according to claim 1 or 2, which is characterized in that the cultivar condition of culture includes: in temperature It is constant temperature incubation under 55-65% for 20-22 DEG C, humidity;Culture is protected from light in 10 days early periods, later period intensity of illumination is 1000-1500 1x。
5. production method according to claim 3, which is characterized in that the cultivar condition of culture includes: to be in temperature 20-22 DEG C, humidity be 55-65% under constant temperature incubation;Culture is protected from light in 10 days early periods, later period intensity of illumination is 1000-1500 1x。
6. according to claim 1, production method described in 2 or 5, which is characterized in that the substituting stuff cultivation condition includes: culture temperature Degree is 28-30 DEG C, relative air humidity 60-75%, and culture is protected from light in 10 days early periods, and later period intensity of illumination is 1000-1500 1x。
7. production method according to claim 3, which is characterized in that the substituting stuff cultivation condition includes: that cultivation temperature is 28-30 DEG C, relative air humidity 60-75%, culture is protected from light in 10 days early periods, later period intensity of illumination is 1000-1500 1x.
8. production method according to claim 4, which is characterized in that the substituting stuff cultivation condition includes: that cultivation temperature is 28-30 DEG C, relative air humidity 60-75%, culture is protected from light in 10 days early periods, later period intensity of illumination is 1000-1500 1x.
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