CN101584284B - Method for cultivating live glossy ganoderma vase landscape - Google Patents
Method for cultivating live glossy ganoderma vase landscape Download PDFInfo
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- CN101584284B CN101584284B CN2009100317400A CN200910031740A CN101584284B CN 101584284 B CN101584284 B CN 101584284B CN 2009100317400 A CN2009100317400 A CN 2009100317400A CN 200910031740 A CN200910031740 A CN 200910031740A CN 101584284 B CN101584284 B CN 101584284B
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- vase
- glossy ganoderma
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Abstract
The invention discloses a method for cultivating live glossy ganoderma vase landscape, which is characterized by comprising the following steps: filling proper amount of porous filler at the bottom ina vase, implanting a temperature-resistant drainage tube and an air duct with built-in filter material, and putting one end of the drainage tube and one end of the air duct at the opening of the vase , positioning the other end of the drainage tube at the bottom of the vase, positioning the other end of the air duct on the slightly lower part of the upper surface of the porous filler, filling a cultivation material into the opening of the vase, pricking one deep strain inoculation hole in the center of the cultivation material, inoculating liquid strain into the strain inoculation hole after sealing, sterilization and cooling, and switching into cultivation management; and regulating the growth conditions required by glossy ganoderma cultivation by adjusting temperature, humidity, illuminance and the like of environment and adding moisture or nutrient fluid in time through the drainage tube to meet the requirement of moisture and nutrition of the cultivation material at different growth stages. The method effectively solves the problems of regulating moisture, nutrient element, oxygen content and the like of glossy ganoderma mycelium and fruiting body at different growth stages, and is easy to operate, and the products are tufted and multibranched and have various shapes and natural beauty.
Description
Technical field
The invention belongs to biological technical field, relate to the breeding method of glossy ganoderma, more particularly, relate to a kind of breeding method of live glossy ganoderma vase landscape.
Background technology
It is raw material that glossy ganoderma vase landscape generally adopts the Wild ganoderma of collection or tame ganoderma lucidum fruitbody, selecting a topic, design, be equipped with mountain stone vegetation according to material, process forms through bonding, finishing, decoration, or take bag is planted in the live body glossy ganoderma immigration vase, make live glossy ganoderma vase landscape.Not only there are the many defectives of artificial trace in these methods, and more main is that glossy ganoderma and vase do not combine together, lack natural beauty, and glossy ganoderma moves bottle and cause matrix or thalline to infect easily, thereby have a strong impact on the growth quality of glossy ganoderma.Though and the employing bottle is planted the defective that method has overcome said method to a certain extent, the bottle method of planting exists vegetative stage lower to the requirement of moisture, but the fruit body stage different in moisture requirement problem high to the low back of the elder generation that has relatively high expectations of moisture.Therefore, the bottle method of planting need solve moisture elder generation and hang down the high problem in back, guarantees the supply and the transportation of suitable transpiration of later stage, promotion nutriment, with the condition of the good growth of assurance glossy ganoderma, thereby obtains glossy ganoderma view well.
Summary of the invention
The objective of the invention is for improving the deficiency that above-mentioned cultivation of glossy ganoderma technology exists, the solution bottle is planted glossy ganoderma needs the high technical problem in the low earlier back of moisture, and a kind of breeding method of live glossy ganoderma vase landscape is provided.
To achieve these goals, the technical scheme that provided of technical solution problem of the present invention is as follows:
A kind of breeding method of live glossy ganoderma vase landscape comprises the steps:
A, filling filler: filler is inserted in the vase, and filler accounts for 1/10 to 1/5 of vase volume;
B, implant drain, wireway: an end is had filter membrane or pipe be built-in with the catheter of filter material and an end and have wireway that filter membrane or pipe be built-in with filter material and place and expose the vase oral area, the other end inserts in the vase respectively, wherein, at the bottom of the catheter port was positioned at bottle, the wireway port was positioned at 1-2cm under the filler upper surface;
Filling of C, cultivate material and sterilization: cultivate material is filled to the vase bottleneck, vertically pricks one in the central authorities of cultivate material with pole and be used for the deep hole that bacterial classification inserts, seal, behind 115 ℃ of high-temperature sterilization 3h, cool off standby with the aseptic film that seals then less than cultivate material thickness 2cm;
D, inoculation bacterial classification: under gnotobasis, untie and seal, bacterial classification is inserted in the vase in the cultivate material by deep hole by 2% of cultivate material mass percent, seal bottleneck;
E, cultivating and managing: it is 60%-70% that the mycelial growth phase temperature is controlled at 24-28 ℃, humidity; Behind the mycelial growth 25-30d, it is 85%-95% that temperature is controlled at 22-26 ℃, humidity, and the suitably growth of Ventilation Control stem, cultivate to the 40-50d, can keep the skin wet, promote the transhipment of nutriment by catheter, stimulate fruiting and promote sporophore growth, the fruit body formation phase progressively strengthens illumination 200-2000lx, and when fruit body began to launch spore in a large number, relative moisture should be controlled at 85%-90%; Cultivation is in time brushed lightly and to be swept the collection spore in the later stage, and in time keep the skin wet or nutrient solution to keep water balance, prolong vegetative period, impel glossy ganoderma to promote cap to increase, and carry out the bonsai production management by regulating the control growing condition; When treating that spore is launched in not regrowth, can inject nutrient solution, place clean place to continue long-term cultivation, go out sesame once more by catheter.
The described filler of above-mentioned steps A be foamed ceramics in the poroid filler, porous pottery, ceramic grain filter, glass tube any.
The described cultivate material of above-mentioned steps C is 85% by cotton seed hulls by mass percentage, and corn flour is 4%, and wheat bran is 8%, and gypsum is 1.2%, and potassium dihydrogen phosphate is 0.3%, and glucose is that 1.5% to form solid content formulated with the water of quality such as solid content again.
Its aperture the best of the described deep hole of above-mentioned steps C is 13～17mm.
The described nutrient solution of above-mentioned steps E by mass percentage by urea be 0.5%, glucose is 3%, potassium dihydrogen phosphate is 0.5%, lime is 1%, magnesium sulfate is 0.2%, VB
1Be 0.01% and surplus be that water is mixed and made into.
Its advantage of the breeding method of a kind of live glossy ganoderma vase landscape of the present invention is: adopt modern biology and conventional bottles scape technology to combine, select the dark vase of pottery for use, the cultivate material of directly packing into has therein avoided glossy ganoderma alive to transplant the problem that easily causes infection.Adopt specific inoculation method, by adding specific filler in the vase bottom, the pre-particular design of implanting catheter and wireway in cultivate material solves the inoculation difficulty that the big throat depth of bottle brings, and controls growth period of hypha moisture and nutrient, cultivate material oxygen content in early stage easily and effectively; Also be beneficial to later stage raising fruiting phase moisture, promoted the fortune that goes up of nutritive element, the extra-nutrition element strengthens the fruiting reserve strength.This method is easy to manage, turn out a small bundle of straw, etc. for silkworms to spin cocoons on shape racemosus, comes in every shape, the collection of natural beauty, intention uniqueness is viewed and admired and the edible live glossy ganoderma vase landscape that combines together.
Description of drawings
Fig. 1: glossy ganoderma vase landscape vase internal placement schematic diagram
Among the figure: 1. vase; 2. wireway; 3. filtering material; 4. catheter; 5. bacterial classification inserts the hole; 6. poroid filler.
Embodiment
Embodiment:
1) filler is filled at the bottle end
Select the dark vase of pottery (dress cultivate material amount is 3kg) for cultivating container, to insert foamed ceramics at the bottle inner bottom part, loadings accounts for 10% of volume.
2) implant drain, wireway
By shown in Figure 1, the mouth of pipe one end is built-in with silica gel wireway that the silica gel catheter of the micropore filter material that polytetrafluoroethylene (PTFE) makes and the mouth of pipe one end be built-in with the micropore filter material that polytetrafluoroethylene (PTFE) makes to be placed and exposes the vase oral area, wherein the catheter other end is inserted at the bottom of the vase at the bottom of the bottle, and the wireway other end is positioned at poroid filling surface and descends 1cm slightly.
3) cultivate material is loaded and sterilization
Packing into by cotton seed hulls in bottle is 85%, and corn flour is 4%, and wheat bran is 8%, and gypsum is 1.2%, and potassium dihydrogen phosphate is 0.3%, and glucose is the formulated cultivate material of water of quality such as 1.5% composition cultivate material solid content and solid content.Keep catheter, wireway to be positioned at the filler middle part during filling, must not press close to a bottle wall, and keep catheter, wireway smooth and easy, do not twist together, until being filled up to bottleneck.The bacterial classification access hole that a diameter is 15 ± 2mm is pricked by the central authorities of cultivate material in bottle, and the degree of depth is to be advisable less than cultivate material thickness 2cm.Seal with the aseptic film that seals, cooling is standby behind 115 ℃ of autoclaving 3h.
4) inoculation bacterial classification
Under the condition of sterile working, by 2% of cultivate material mass percent, liquid spawn flows in the bacterial classification access hole via the surface during inoculation, seals bottleneck with bacterial classification.
5) cultivating and managing
It is 60%-70% that the mycelial growth phase temperature is controlled at 24-28 ℃, humidity; Behind the mycelial growth 25-30d, it is 85%-95% that temperature is controlled at 22-26 ℃, humidity, and the suitably growth of Ventilation Control stem, be cultured to about 40-50d, can keep the skin wet, promote the transhipment of nutriment by catheter, stimulate fruiting and promote sporophore growth, the fruit body formation phase progressively strengthens illumination 200-2000lx, and when fruit body began to launch spore in a large number, relative moisture should be controlled at 85%-90%; The cultivation later stage in time brushes lightly and sweeps the collection spore, and in time keep the skin wet or nutrition to keep water balance, prolong the cycle in vegetative period, impel glossy ganoderma to promote cap to increase, and carry out the bonsai production management by regulating the control growing condition, form a small bundle of straw, etc. for silkworms to spin cocoons on shape racemosus, come in every shape, the live glossy ganoderma vase landscape of natural beauty.When treating that spore is launched in not regrowth, by catheter inject by urea be 0.5%, glucose is 3%, potassium dihydrogen phosphate is 0.5%, lime is 1%, magnesium sulfate is 0.2%, VB1 be 0.01% and surplus be the nutrient solution that water is mixed with, place clean place to continue long-term the cultivation, go out sesame once more.
Present embodiment, the cultivate material amount is big, the fruiting longer duration, product a small bundle of straw, etc. for silkworms to spin cocoons on is strong colourful, and view is graceful naturally.
Claims (5)
1. the breeding method of a live glossy ganoderma vase landscape is characterized in that, comprises the steps:
A, filling filler: filler is inserted in the vase, and filler accounts for 1/10 to 1/5 of vase volume;
B, implant drain, wireway: an end is had filter membrane or pipe be built-in with the catheter of filter material and an end and have wireway that filter membrane or pipe be built-in with filter material and place and expose the vase oral area, the other end inserts in the vase respectively, wherein, at the bottom of the catheter port was positioned at bottle, the wireway port was positioned at 1-2cm under the filler upper surface;
Filling of C, cultivate material and sterilization: cultivate material is filled to bottleneck, vertically pricks one in the central authorities of cultivate material with pole and be used for the deep hole that bacterial classification inserts, seal, behind 115 ℃ of high-temperature sterilization 3h, cool off standby with the aseptic film that seals then less than cultivate material thickness 2cm;
D, inoculation bacterial classification: under gnotobasis, untie and seal, bacterial classification is inserted by deep hole by 2% of cultivate material mass percent bacterial classification inserts in the hole in the vase, seal bottleneck;
E, cultivating and managing: it is 60%-70% that the mycelial growth phase temperature is controlled at 24-28 ℃, humidity; Behind the mycelial growth 25-30d, it is 85%-95% that temperature is controlled at 22-26 ℃, humidity, and the suitably growth of Ventilation Control stem, cultivate to the 40-50d, keep the skin wet, promote the transhipment of nutriment by catheter, stimulate fruiting and promote sporophore growth, the fruit body formation phase progressively strengthens illumination 200-2000lx, and when fruit body began to launch spore in a large number, relative moisture should be controlled at 85%-90%; Cultivation is in time brushed lightly and to be swept the collection spore in the later stage, and in time keep the skin wet or nutrition to keep water balance, prolong the cycle in vegetative period, impel glossy ganoderma to promote cap to increase, and carry out the bonsai production management by regulating the control growing condition; When treating that spore is launched in not regrowth, inject nutrient solution, place clean place to continue long-term the cultivation, go out sesame once more by catheter.
2. the breeding method of a kind of live glossy ganoderma vase landscape according to claim 1 is characterized in that, the described filler of steps A be in the apotrophic poroid filler foamed ceramics, porous pottery, ceramic grain filter, glass tube any.
3. the breeding method of a kind of live glossy ganoderma vase landscape according to claim 1, it is characterized in that, the described cultivate material of step C is 85% by cotton seed hulls by mass percentage, corn flour is 4%, wheat bran is 8%, gypsum is 1.2%, and potassium dihydrogen phosphate is 0.3%, and glucose is that 1.5% to form solid content formulated with the water of quality such as solid content again.
4. the breeding method of a kind of live glossy ganoderma vase landscape according to claim 1 is characterized in that, its aperture the best of the described deep hole of step C is 13～17mm.
5. the breeding method of a kind of live glossy ganoderma vase landscape according to claim 1, it is characterized in that, the described nutrient solution of step e for by mass percentage by urea be 0.5%, glucose is 3%, potassium dihydrogen phosphate is 0.5%, lime is 1%, magnesium sulfate is 0.2%, VB
1Be 0.01% and surplus be that water is mixed and made into.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009100317400A CN101584284B (en) | 2009-07-03 | 2009-07-03 | Method for cultivating live glossy ganoderma vase landscape |
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| CN2009100317400A CN101584284B (en) | 2009-07-03 | 2009-07-03 | Method for cultivating live glossy ganoderma vase landscape |
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| CN101584284A CN101584284A (en) | 2009-11-25 |
| CN101584284B true CN101584284B (en) | 2010-12-08 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102145632B (en) * | 2010-11-19 | 2012-10-10 | 安徽师范大学 | Environment-friendly automobile interior |
| CN102318505B (en) * | 2011-08-18 | 2013-03-20 | 无锡飞凤生物科技有限公司 | Ganoderma lucidum potted landscape cultivation method |
| CN103621312B (en) * | 2013-11-28 | 2015-12-09 | 熊家明 | A kind of take stone as glossy ganoderma dish garden of pedestal and preparation method thereof |
| CN104041326A (en) * | 2014-06-27 | 2014-09-17 | 桐城市牯牛背农业开发有限公司 | Fast cultivation method for ganoderma lucidum |
| CN104195017A (en) * | 2014-09-15 | 2014-12-10 | 董学堂 | Bottled fresh ganoderma lucidum wine and cultivation and production process thereof |
| CN110521489A (en) * | 2016-10-14 | 2019-12-03 | 北京京诚生物科技有限公司 | The method for extending living body glossy ganoderma dish garden storage life and improving its preservation quality |
| CN107471896A (en) * | 2017-09-11 | 2017-12-15 | 锁现民 | A kind of technique that ganoderma lucidum is quickly produced in vial |
| CN114667891A (en) * | 2022-03-16 | 2022-06-28 | 重庆华绿生物有限公司 | Novel edible mushroom cultivation bottle and use method thereof |
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|---|---|---|---|---|
| CN1318280A (en) * | 2000-04-18 | 2001-10-24 | 宁阳县泰山赤灵芝盆景艺术研究所 | Cultivation of Glossy Ganoderma potted landscape |
| CN2488718Y (en) * | 2001-07-26 | 2002-05-01 | 浙江省农业科学院花卉研究开发中心 | Ecotype bottle-scenery |
| CN1545839A (en) * | 2003-12-11 | 2004-11-17 | 湖南农大园林发展中心 | Cultivation method of alive Glossy Ganoderma potted landscape |
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Patent Citations (3)
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|---|---|---|---|---|
| CN1318280A (en) * | 2000-04-18 | 2001-10-24 | 宁阳县泰山赤灵芝盆景艺术研究所 | Cultivation of Glossy Ganoderma potted landscape |
| CN2488718Y (en) * | 2001-07-26 | 2002-05-01 | 浙江省农业科学院花卉研究开发中心 | Ecotype bottle-scenery |
| CN1545839A (en) * | 2003-12-11 | 2004-11-17 | 湖南农大园林发展中心 | Cultivation method of alive Glossy Ganoderma potted landscape |
Non-Patent Citations (3)
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| JP特开2008-228674A 2008.10.02 |
| 武镇京.灵芝瓶景的栽培.《中国花卉盆景》.1998,第24页. * |
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Address after: 212028 Zhenjiang, Dantu Metro Industrial Park Rui East Road, No. 9 Patentee after: Jiangsu University of Science and Technology Address before: 212003 Zhenjiang,, Jiangsu, Jiangsu University of Science and Technology, No. Patentee before: Jiangsu University of Science and Technology |
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