CN105238699A - Preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit - Google Patents
Preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit Download PDFInfo
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- CN105238699A CN105238699A CN201510587382.7A CN201510587382A CN105238699A CN 105238699 A CN105238699 A CN 105238699A CN 201510587382 A CN201510587382 A CN 201510587382A CN 105238699 A CN105238699 A CN 105238699A
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Abstract
The invention discloses a preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit. The preparation method includes: activating solid spawns of the agrocybe aegirit, taking original inoculation blocks after aerial mycelia grow to positions 2-3cm away from the original inoculation blocks, inoculating the inoculation blocks into a shake flask containing a liquid culture medium, performing shaking culture till mycelium pellets appear, inoculating liquid spawns into a fermentation medium of a fermentation tank, feeding clean air at the temperature of 20-25 DEG C and culturing for 8-10 days so as to obtain the liquid fermentation spawns for industrial cultivation of the agrocybe aegirit. The preparation method is simple, production cycle is short, and the spawns are high in purity, strong in vitality, quick to germinate and suitable for extensive popularization and application.
Description
Technical field
The present invention relates to edible fungi liquid strain preparation field, be specifically related to a kind of preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification.
Background technology
Tea tree mushroom is nutritious, delicious flavour, and stem is tender and crisp, and mouthfeel is fabulous, is well-known edible mushrooms, and has very high pharmaceutical use, and be called " Chinese refreshing mushroom " by people, market demand increases sharply in recent years.Tea tree mushroom domestication is very short with the cultivation successful time, although tame output increases by a fairly big margin in recent years, but because its cycle of gathering is long, technique is relatively loaded down with trivial details, still be in the elementary pocket type cultivation stage at present, the impact by season is very large, does not realize large batch of factory culture, and the solid spawn that bacterial classification is also still traditional, production efficiency is very low.The general mode of production of traditional solid spawn is: prepare bacterium culture medium according to formula, substratum is loaded seed bottle, bacterial classification is accessed after sterilizing, cultivate about 30 ~ 40 days bacterial classifications to grow, solid spawn is scraped out fragment, manual operations access cultivating bag is cultivated, the spawn culture time is long, each seed bottle can only inoculate at most tens cultivating bags, need prepare a large amount of seed bottle, and after inoculation, cultivating bag mycelium germination point is few, germination physiology is slow, the insect pest of easy generation miscellaneous bacteria, culture cycle is long, and factory culture is difficult to realize.
At present, mushroom industry has entered fast-developing period, and realizing annual, large-scale production has been main trend, and traditional solid spawn mode of production will gradually to liquid spawn transition.Liquid spawn, because its purity is high, energetic, it is fast to sprout, will obtain promotion and application more widely in Edible Fungi.Current tea tree mushroom factory culture liquid spawn research aspect is still blank.Based on above reason, developing that a kind of technical process is simple, the cycle is shorter, be convenient to inoculate, can carry out the preparation method of the tea tree mushroom liquid spawn of mechanized operation has been imperative.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification, this preparation method is simple, gained liquid spawn have purity high, energetic, sprout fast, growth cycle is short, fruiting is neat, inoculation is convenient, be convenient to manage, applicable factorial praluction.
For solving prior art problem, the technical scheme that the present invention takes is:
A kind of preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification, comprise the following steps: first tea tree mushroom solid spawn is activated, when aerial hyphae grows to from former inoculation block 2 ~ 3cm, get bacterial classification block to receive and fill in the shaking flask of liquid nutrient medium, after shaking culture to mycelium pellet occurs, again liquid spawn is connected in fermentor tank in fermention medium, passes into uncontaminated air and cultivate at 20 ~ 25 DEG C the tea tree mushroom liquid fermenting bacterial classification that 8 ~ 10 days obtain factory culture.
As preparation method preferably, the component of described liquid nutrient medium is: wheat-flour 10 ~ 20g, white sugar 10 ~ 20g, yeast powder 2 ~ 5g, magnesium sulfate 1 ~ 2g, potassium primary phosphate 1 ~ 2g, purified water 1000mL, pH5.8 ~ 6.0.
As preparation method preferably, in described fermentor tank, liquid nutrient medium component is: wheat-flour 10 ~ 20g, white sugar 10 ~ 20g, yeast powder 2 ~ 5g, magnesium sulfate 1 ~ 2g, potassium primary phosphate 1 ~ 2g, defoamer 0.05 ~ 0.10mL, purified water 1000mL, pH5.8 ~ 6.0.
As preparation method preferably, described shaking culture temperature is 20 ~ 25 DEG C, rotating speed 130 ~ 150rpm.
As preparation method preferably, described uncontaminated air is dry uncontaminated air.
As preparation method preferably, the pressure of described uncontaminated air is at 0.05 ~ 0.20MPa.
beneficial effect
Compared with prior art, preparation method of the present invention is simple, with short production cycle, be convenient to inoculation and mechanized operation, the liquid fermenting bacterial classification purity of gained of the present invention is high in addition, energetic, sprout fast, growth cycle is short, and fruiting is neat, inoculation is convenient, be convenient to management, be applicable to the advantages such as factorial praluction, the germination point quantity of cultivar can be ensured, can significantly shorten the production of hybrid seeds time again.The present invention can in the large scale application of edible mushrooms industry.
Accompanying drawing explanation
Fig. 1 is the process flow sheet of the preparation of tea tree mushroom liquid fermenting bacterial classification and factory culture application.
Embodiment
Below in conjunction with embodiment, the invention will be further described.Following examples just for performance of the present invention is clearly described, and only can not be confined to the following examples.
embodiment 1
prepared by liquid fermenting bacterial classification
Obtain tea tree mushroom solid spawn, activation expansion is connected to test tube, and (in vitro substratum is solid PDA medium: potato (peeling) 200g, glucose 20g, agar 20g, purified water 1000mL, pH nature) in, be placed in the biochemical cultivation case of 25 DEG C and cultivate, continuous subculture several times, obtains stable test tube kind; (substratum is that solid PDA fills a prescription: potato (peeling) 200g stable test tube strains to be forwarded to culture dish, glucose 20g, agar 20g, purified water 1000mL, pH nature) in activate, be placed in the biochemical cultivation case of 25 DEG C and cultivate, treat that aerial hyphae grows to culture dish edge and can use; With punch tool, the culture dish grown being got inoculation block is forwarded to containing liquid nutrient medium (wheat-flour 10g, white sugar 10g, yeast powder 2g, magnesium sulfate 1g, potassium primary phosphate 1g, purified water 1000mL, pH5.8 ~ 6.0) triangular flask in, be placed in 25 DEG C, carry out constant-temperature shaking culture in the shaking table of 150rpm 10 days; Liquid spawn is connected to cultivation in fermentation cylinder for fermentation substratum when growing up to mycelium pellet by the mycelia in triangular flask, and (liquid culture based formulas is: wheat-flour 10g, white sugar 10g, yeast powder 2g, magnesium sulfate 1g, potassium primary phosphate 1g, defoamer 0.10mL, purified water 1000mL, pH5.8 ~ 6.0), pass into fermentor tank the dried and clean air that pressure is 0.05MPa homo(io)thermism, the tea tree mushroom liquid fermenting bacterial classification that can obtain applicable factory culture for 8 days cultivated by fermentor tank under 22 DEG C of conditions.
factory culture is implemented
Culture medium for cultivating: according to the every batch cultivation base weight demands producing regulation, according to cotton seed hulls 49 parts, corn cob 24 parts, 15 parts, wheat bran, Semen Maydis powder 5 parts, beet pulp 5 parts, the ratio that oyster shell whiting is 2 parts takes raw material.
Add water, stir: various starting material are dropped into stirrer successively, first dry mixing 15 minutes, then the stirring that adds water, until the various raw materials in substratum mix, fully absorb water, requiring the water ratio of substratum to be 66% ~ 68%, pH is 6.2 ~ 6.8.
Bottling, lid bottle cap: every 16 acrylic plastering culture bottles are one basket, fully automatic bottle filling machine is adopted to be filled in culture bottle by the substratum prepared, make a call to altogether at the bottom of 5 holes to bottle by automatic punch in charge level centre and surrounding after filling end, require that substratum charge level is smooth, culture bottle capacity 1200mL, the charge amount of every bottle is 900g ~ 950g.Filling, punched after, be the bottle cap that culture bottle covers coupling by automatic cover lid machine.
High pressure steam sterilization, cooling: be placed on tote cart by the cultivation basket that culture bottle is housed, push in autoclave sterilizer and carry out sterilizing, and 121 DEG C maintain 90min.After sterilizing terminates, culture bottle is put into cooling room and carry out pressure cooling, inoculate when material temperature is reduced to room temperature.
Inoculation
Tea tree mushroom liquid fermenting bacterial classification is accessed liquid spawn by fully automatic inoculating machine under laminar flow hood in culture bottle, and every bottle of inoculum size is 25 ~ 30mL.
Mycelium culture, mycelium stimulation
After inoculation terminates, culture bottle is moved into culturing room and carry out mycelium culture, culture environment is: room temperature 20 ~ 22 DEG C, atmospheric moisture 60% ~ 70%, lucifuge, air cycle are good.Culturing room adopts automatic control system, and control material temperature at 22 ~ 25 DEG C, space humidity controls at 60% ~ 70%, CO
2concentration controls at 2000 ~ 4000ppm, guarantees clean environment degree by high-efficiency air filtering simultaneously.Cultivate mycelia after 20 ~ 25 days and can cover with culture bottle.
The culture bottle covering with mycelia is moved into mycelium stimulation workshop, carries out mycelium stimulation process with automatic mycelium stimulation machine, remove the mycelia that charge level is aging, form mechanical stimulus simultaneously, promote formation and the differentiation of the former base of tea plant mushroom fruit body.Mycelium stimulation terminate after charge level water spray 10 ~ 15mL, make charge level keep moistening.
Management of producing mushroom
By culture bottle move into fertility room, the room temperatures of 10 ~ 26 DEG C, 85% ~ 95% atmospheric moisture, CO
2concentration carries out urging flower bud process under 3000 ~ 4000ppm condition, charge level charge level mycelia overstrike after 5 ~ 7 days, and after urging flower bud to terminate, the illumination and the day and night temperature that carry out 500Lux intensity every day stimulate in good time, and by CO
2concentration controls to make mushroom blastogenesis long neat, consistent at 3000 ~ 8000ppm, and within 10 ~ 12 days, former base is obvious.
When mushroom bud grows bottleneck 1 ~ 2cm, reduce ventilation, improve the CO of environment
2concentration is to more than 10000ppm, promote the elongation of tea tree mushroom stem, carry out the illumination of 500Lux intensity every day according to mushroom reguarity and day and night temperature stimulates in good time, Xanthophyll cycle and thermal stimulation are carried out to tea tree mushroom, make that tea tree mushroom growing way is consistent, fruiting neat, mushroom clump is larger.
Gather
Can gather when tea tree mushroom mushroom cap is hemispherical.Enter the planting time about 15 ~ 18 days of fertility room.The average per unit area yield of first tide is 110 ~ 120g/ bottle.
Gather after terminating, cleaning charge level also scratches charge level 1 ~ 2cm planting material, and supplement moisture content, stronger ventilation amount, culture temperature is increased to 22 ~ 25 DEG C, and its mycelia is recovered, and within 10 ~ 15 days, goes out the second damp mushroom simultaneously.The average per unit area yield of second tide is 130 ~ 140g/ bottle.
Every bottle goes out two damp mushrooms, and every bottle is produced fresh mushroom 240 ~ 260g.
embodiment 2
prepared by liquid fermenting bacterial classification
Obtain tea tree mushroom solid spawn, activation expansion is connected in test tube (in vitro substratum is solid PDA medium: potato (peeling) 200g, glucose 20g, agar 20g, purified water 1000mL, pH nature), is placed in the biochemical cultivation case of 25 DEG C and cultivates.Continuous subculture several times, obtains stable test tube kind; (substratum is that solid PDA fills a prescription: potato (peeling) 200g stable test tube strains to be forwarded to culture dish, glucose 20g, agar 20g, purified water 1000mL, pH nature) in activate, be placed in the biochemical cultivation case of 25 DEG C and cultivate, treat that aerial hyphae grows to culture dish edge and can use; With punch tool, the culture dish grown being got inoculation block is forwarded to containing liquid nutrient medium (wheat bran 10g, white sugar 10g, peptone 2g, magnesium sulfate 1g, potassium primary phosphate 1g, purified water 1000mL, pH5.8 ~ 6.0) triangular flask in, be placed in 25 DEG C, carry out constant-temperature shaking culture in the shaking table of 150rpm 10 days; Liquid spawn is connected to fermentation cylinder for fermentation cultivation when growing up to mycelium pellet by the mycelia in triangular flask, and (fermentative medium formula is: wheat bran 10g, white sugar 10g, peptone 2g, magnesium sulfate 1g, potassium primary phosphate 1g, defoamer 0.10mL, purified water 1000mL, pH5.8 ~ 6.0), pass into fermentor tank the dried and clean air that pressure is 0.05MPa homo(io)thermism, the tea tree mushroom liquid fermenting bacterial classification that can obtain applicable factory culture for 8 days cultivated by fermentor tank under 22 DEG C of conditions.
factory culture is implemented
Culture medium for cultivating: according to the every batch cultivation base weight demands producing regulation, according to cotton seed hulls 39 parts, corn cob 24 parts, 15 parts, wheat bran, 10 parts, rice bran, Semen Maydis powder 5 parts, beet pulp 5 parts, the ratio that oyster shell whiting is 2 parts takes raw material.
Add water, stir: various starting material are dropped into stirrer successively, first dry mixing 15 minutes, then the stirring that adds water, until the various raw materials in substratum mix, fully absorb water, requiring the water ratio of substratum to be 66% ~ 68%, pH is 6.2 ~ 6.8.
Bottling, lid bottle cap: every 16 acrylic plastering culture bottles are one basket, fully automatic bottle filling machine is adopted to be filled in culture bottle by the substratum prepared, make a call to altogether at the bottom of 5 holes to bottle by automatic punch in charge level centre and surrounding after filling end, require that substratum charge level is smooth, culture bottle capacity 1200mL, the charge amount of every bottle is 900g ~ 950g.Filling, punched after, be the bottle cap that culture bottle covers coupling by automatic cover lid machine.
High pressure steam sterilization, cooling: be placed on tote cart by the cultivation basket that culture bottle is housed, push in autoclave sterilizer and carry out sterilizing, and 121 DEG C maintain 90min.After sterilizing terminates, culture bottle is put into cooling room and carry out pressure cooling, inoculate when material temperature is reduced to room temperature.
Inoculation
Tea tree mushroom liquid fermenting bacterial classification is accessed liquid spawn by fully automatic inoculating machine under laminar flow hood in culture bottle, and every bottle of inoculum size is 25 ~ 30mL.
Mycelium culture, mycelium stimulation
After inoculation terminates, culture bottle is moved into culturing room and carry out mycelium culture, culture environment is: room temperature 20 ~ 22 DEG C, atmospheric moisture 60% ~ 70%, lucifuge, air cycle are good.Culturing room adopts automatic control system, and control material temperature at 22 ~ 25 DEG C, space humidity controls at 60% ~ 70%, CO
2concentration controls at 2000 ~ 4000ppm, guarantees clean environment degree by high-efficiency air filtering simultaneously.Cultivate mycelia after 20 ~ 25 days and can cover with culture bottle.
The culture bottle covering with mycelia is moved into mycelium stimulation workshop, carries out mycelium stimulation process with automatic mycelium stimulation machine, remove the mycelia that charge level is aging, form mechanical stimulus simultaneously, promote formation and the differentiation of the former base of tea plant mushroom fruit body.Mycelium stimulation terminate after charge level water spray 10 ~ 15mL, make charge level keep moistening.
Management of producing mushroom
By culture bottle move into fertility room, the room temperatures of 10 ~ 26 DEG C, 85% ~ 95% atmospheric moisture, CO
2concentration carries out urging flower bud process under 3000 ~ 4000ppm condition, charge level charge level mycelia overstrike after 5 ~ 7 days, and after urging flower bud to terminate, the illumination and the day and night temperature that carry out 500Lux intensity every day stimulate in good time, and by CO
2concentration controls to make mushroom blastogenesis long neat, consistent at 3000 ~ 8000ppm, and within 10 ~ 12 days, former base is obvious.
When mushroom bud grows bottleneck 1 ~ 2cm, reduce ventilation, improve the CO of environment
2concentration is to more than 10000ppm, promote the elongation of tea tree mushroom stem, carry out the illumination of 500Lux intensity every day according to mushroom reguarity and day and night temperature stimulates in good time, Xanthophyll cycle and thermal stimulation are carried out to tea tree mushroom, make that tea tree mushroom growing way is consistent, fruiting neat, mushroom clump is larger.
Gather
Can gather when tea tree mushroom mushroom cap is hemispherical.Enter the planting time about 15 ~ 18 days of fertility room.The average per unit area yield of first tide is 110 ~ 120g/ bottle.
Gather after terminating, cleaning charge level also scratches charge level 1 ~ 2cm planting material, and supplement moisture content, stronger ventilation amount, culture temperature is increased to 22 ~ 25 DEG C, and its mycelia is recovered, and within 10 ~ 15 days, goes out the second damp mushroom simultaneously.The average per unit area yield of second tide is 100 ~ 120g/ bottle.
Every bottle goes out two damp mushrooms, and every bottle is produced fresh mushroom 180 ~ 200g.
comparative example
prepared by solid spawn
Obtain tea tree mushroom solid spawn, activation expansion is connected in test tube (in vitro substratum is solid PDA medium: potato (peeling) 200g, glucose 20g, agar 20g, purified water 1000mL, pH nature), is placed in the biochemical cultivation case of 25 DEG C and cultivates.Continuous subculture several times, obtains stable test tube kind.Stable test tube strains is forwarded to vial (culture medium prescription: dry weed tree sawdust 55 parts, cotton seed hulls 23 parts, 20 parts, wheat bran, white sugar 1 part, light calcium carbonate 1 part, water ratio 55%-60%), be 23-25 DEG C in temperature, humidity is cultivate 30-40 days in 50-60% clean environment, and in vial, mycelia is covered with and can obtain original seed.Original seed is accessed (culture medium prescription: dry weed tree sawdust 58 parts in plastics bag, cotton seed hulls 20 parts, 20 parts, wheat bran, white sugar 1 part, light calcium carbonate 1 part, water ratio 55%-60%), be 23-25 DEG C in temperature, humidity is cultivate 25-35 days in 50-60% clean environment, and bacterial classification covers with and can obtain cultivar, then the mycelia after-ripening carried out 7 days can use.
factory culture is implemented
Culture medium for cultivating: according to the every batch cultivation base weight demands producing regulation, according to cotton seed hulls 39 parts, corn cob 24 parts, 15 parts, wheat bran, 10 parts, rice bran, Semen Maydis powder 5 parts, beet pulp 5 parts, the ratio that oyster shell whiting is 2 parts takes raw material.
Add water, stir: various starting material are dropped into stirrer successively, first dry mixing 15 minutes, then the stirring that adds water, until the various raw materials in substratum mix, fully absorb water, requiring the water ratio of substratum to be 66% ~ 68%, pH is 6.2 ~ 6.8.
Bottling, lid bottle cap: every 16 acrylic plastering culture bottles are one basket, fully automatic bottle filling machine is adopted to be filled in culture bottle by the substratum prepared, make a call to altogether at the bottom of 5 holes to bottle by automatic punch in charge level centre and surrounding after filling end, require that substratum charge level is smooth, culture bottle capacity 1200mL, the charge amount of every bottle is 900g ~ 950g.Filling, punched after, be the bottle cap that culture bottle covers coupling by automatic cover lid machine.
High pressure steam sterilization, cooling: be placed on tote cart by the cultivation basket that culture bottle is housed, push in autoclave sterilizer and carry out sterilizing, and 121 DEG C maintain 90min.After sterilizing terminates, culture bottle is put into cooling room and carry out pressure cooling, inoculate when material temperature is reduced to room temperature.
Inoculation
By in tea tree mushroom solid spawn manual access culture bottle, every bottle of inoculum size is 15 ~ 20 grams.
Mycelium culture, mycelium stimulation
After inoculation terminates, culture bottle is moved into culturing room and carry out mycelium culture, culture environment is: room temperature 20 ~ 22 DEG C, atmospheric moisture 60% ~ 70%, lucifuge, air cycle are good.Culturing room adopts automatic control system, and control material temperature at 22 ~ 25 DEG C, space humidity controls at 60% ~ 70%, CO
2concentration controls at 2000 ~ 4000ppm, guarantees clean environment degree by high-efficiency air filtering simultaneously.Cultivate mycelia after 50 ~ 60 days and can cover with culture bottle.
The culture bottle covering with mycelia is moved into mycelium stimulation workshop, carries out mycelium stimulation process with automatic mycelium stimulation machine, remove the mycelia that charge level is aging, form mechanical stimulus simultaneously, promote formation and the differentiation of the former base of tea plant mushroom fruit body.Mycelium stimulation terminate after charge level water spray 10 ~ 15mL, make charge level keep moistening.
Management of producing mushroom
By culture bottle move into fertility room, the room temperatures of 10 ~ 26 DEG C, 85% ~ 95% atmospheric moisture, CO
2concentration carries out urging flower bud process under 3000 ~ 4000ppm condition, charge level charge level mycelia overstrike after 5 ~ 7 days, and after urging flower bud to terminate, the illumination and the day and night temperature that carry out 500Lux intensity every day stimulate in good time, and by CO
2concentration controls to make mushroom blastogenesis long neat, consistent at 3000 ~ 8000ppm, and within 10 ~ 12 days, former base is obvious.
When mushroom bud grows bottleneck 1 ~ 2cm, reduce ventilation, improve the CO of environment
2concentration is to more than 10000ppm, promote the elongation of tea tree mushroom stem, carry out the illumination of 500Lux intensity every day according to mushroom reguarity and day and night temperature stimulates in good time, Xanthophyll cycle and thermal stimulation are carried out to tea tree mushroom, make that tea tree mushroom growing way is consistent, fruiting neat, mushroom clump is larger.
Gather
Can gather when tea tree mushroom mushroom cap is hemispherical.Enter the planting time about 15 ~ 18 days of fertility room.The average per unit area yield of first tide is 70 ~ 90g/ bottle.
Gather after terminating, cleaning charge level also scratches charge level 1 ~ 2cm planting material, and supplement moisture content, stronger ventilation amount, culture temperature is increased to 22 ~ 25 DEG C, and its mycelia is recovered, and within 10 ~ 15 days, goes out the second damp mushroom simultaneously.The average per unit area yield of second tide is 90 ~ 120g/ bottle.
Every bottle goes out two damp mushrooms, and every bottle is produced fresh mushroom 160 ~ 180g.
Above step describes the preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification, cultivate with the method for the invention, liquid spawn makes simple, and bacterial classification firepower is strong, sprouts fast, to gather two damp whole cultivation period about 25 ~ 33 days, on average gather per unit area yield more than 250 grams, and biological transformation ratio reaches about 85 ~ 100%, and reguarity is better, collection period Relatively centralized, exterior of commodity is better.And technology planted by general tea tree mushroom solid spawn bag, not only bacterial classification makes complicated, cycle is longer, occupy a large amount of spaces, and need Hand Inoculations, significantly increase the risk of pollution, spawn activity is weak, in about 50 ~ 60 days mycelium culture cycle after inoculation, generally reach 6 months collection period even 1 year, the advantage of factorial praluction cannot be played.
The foregoing is only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (6)
1. the preparation method of a tea tree mushroom factory culture liquid fermenting bacterial classification, it is characterized in that, comprise the following steps: first tea tree mushroom solid spawn is activated, when aerial hyphae grows to from former inoculation block 2 ~ 3cm, get bacterial classification block to receive and fill in the shaking flask of liquid nutrient medium, after shaking culture to mycelium pellet occurs, then liquid spawn is connected in fermentor tank in fermention medium, passes into uncontaminated air and cultivate at 20 ~ 25 DEG C the tea tree mushroom liquid fermenting bacterial classification that 8 ~ 10 days obtain factory culture.
2. the preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification according to claim 1, it is characterized in that, the component of described liquid nutrient medium is: wheat-flour 10 ~ 20g, white sugar 10 ~ 20g, yeast powder 2 ~ 5g, magnesium sulfate 1 ~ 2g, potassium primary phosphate 1 ~ 2g, purified water 1000mL, pH5.8 ~ 6.0.
3. the preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification according to claim 1, it is characterized in that, described fermention medium component is: wheat-flour 10 ~ 20g, white sugar 10 ~ 20g, yeast powder 2 ~ 5g, magnesium sulfate 1 ~ 2g, potassium primary phosphate 1 ~ 2g, defoamer 0.05 ~ 0.10mL, purified water 1000mL, pH5.8 ~ 6.0.
4. the preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification according to claim 1, is characterized in that: described shaking culture temperature is 20 ~ 25 DEG C, rotating speed 130 ~ 150rpm.
5. the preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification according to claim 1, is characterized in that: described uncontaminated air is dry uncontaminated air.
6. the preparation method of tea tree mushroom factory culture liquid fermenting bacterial classification according to claim 1, is characterized in that: the pressure of described uncontaminated air is at 0.05 ~ 0.20MPa.
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| CN108575564A (en) * | 2018-06-19 | 2018-09-28 | 贵州省贵福菌业发展有限公司 | a kind of oil tea mushroom culture medium |
| CN108834764A (en) * | 2018-06-19 | 2018-11-20 | 贵州省贵福菌业发展有限公司 | A kind of preparation method of oil tea mushroom activated liquid strain |
| CN109479622A (en) * | 2018-11-15 | 2019-03-19 | 江苏强农农业技术服务有限公司 | A kind of tea tree mushroom strains industrial production method |
| CN115119688A (en) * | 2022-04-22 | 2022-09-30 | 中华全国供销合作总社昆明食用菌研究所 | White agrocybe chaxingu strain and its selective breeding and industrial cultivation method |
| CN115119688B (en) * | 2022-04-22 | 2023-05-30 | 中华全国供销合作总社昆明食用菌研究所 | White agrocybe aegerita strain and breeding and industrial cultivation method thereof |
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