CN108575564A - a kind of oil tea mushroom culture medium - Google Patents
a kind of oil tea mushroom culture medium Download PDFInfo
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- CN108575564A CN108575564A CN201810631228.9A CN201810631228A CN108575564A CN 108575564 A CN108575564 A CN 108575564A CN 201810631228 A CN201810631228 A CN 201810631228A CN 108575564 A CN108575564 A CN 108575564A
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- oil tea
- culture medium
- tea mushroom
- sawdust
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of oil tea mushroom culture mediums, belong to planting edible mushroom technical field, include that following raw material forms according to parts by weight：15 25 parts of sawdust, 58 parts of ginkgo leaf, 25 parts of analysis for soybean powder, oily 36 parts of bran, 26 parts of oyster shell whiting, 0.3 1 parts of potassium dihydrogen phosphate, 0.3 1 parts of magnesium sulfate, 13 parts of peptone powder, 13 parts of agar.Oil tea mushroom is planted using the culture medium that the present invention makes, raw material sawdust is handled, structure is more loose, and ventilation is preferable, so that oil tea mushroom is easier to decompose nutritional ingredient therein, meanwhile more GINKGO BILOBA EXTRACT and lactones in the ginkgo leaf of addition, coordinate the oyster shell whiting after specially treated, and other raw materials, it is greatly improved the raising yield of oil tea mushroom, can guarantee that the growth of oil tea mushroom is vigorous, the body of rod of bacterial strain is not only sturdy but also length is more than common oil tea mushroom.
Description
Technical field
The invention belongs to edible mushroom field more particularly to a kind of oil tea mushroom culture mediums.
Background technology
Oil tea mushroom category Basidiomycotina, Fen Xiu umbrellas section are a kind of edible mushrooms developed in recent years.Oil tea mushroom contains needed by human body
A variety of amino acid, such as 8 kinds of amino acid that glutamic acid, asparatate and glycine, especially human body itself cannot synthesize, this
Outside, the several mineral materials such as the oil tea mushroom also glucan containing needed by human body, mycoprotein, B family vitamin, iron, potassium, zinc and selenium member
Element.Oil tea mushroom total reducing sugar is up to 54.36%, raw sugar up to 29.96%, and for crude protein up to 27.51%, protein content is up to 19.55%, it is above-mentioned this
A little active ingredients are obviously higher by other mushrooms.Oil tea mushroom delicious flavour, gives off a strong fragrance, fresh and crisp, no matter cooks soup or cooking,
All it is a good selection.And theory of traditional Chinese medical science thinks, oil tea mushroom is mild-natured, Gan Wen, nontoxic, have kidney tonifying, diuresis, invigorating the spleen,
Antidiarrheal and other effects all has kidney deficiency, frequent micturition, oedema and rheumatism and obese patient etc. ideal effect, to children's low-heat, urine
Bed etc. also has the treatment function of auxiliary.Since oil tea mushroom nutritive value is high, in good taste, therefore, oil tea mushroom has become people at present
Comparatively ideal health food is delicious rare one of edible mushroom.
At present oil tea mushroom large area plant, but due to strain degeneration, culture substrate be formulated unreasonable, bag making at
The reasons such as low, the disease pest harm of product rate cause yield not high.The present invention focuses on to study the formula of culture substrate, in the prior art, such as
Patent CN201711318955.1 is disclosed makes base with rape stalk slag, corn flour, cotton seed hulls, calcium carbonate, lime, wheat bran, sugar
Matter.Patent CN201410556228.9 is disclosed with peach sawdust, willow sawdust, camellia shell, analysis for soybean powder, potassium dihydrogen phosphate, mountain
Tea oil bran, magnesium sulfate, agar strip, gypsum, antifoaming agent, PH conditioning agents are that raw material makes matrix.Certain effect can be played, but is imitated
Fruit is less desirable.
Invention content
In order to solve the above technical problems, the present invention provides a kind of oil tea mushroom culture medium, concrete scheme is as follows：
A kind of oil tea mushroom culture medium includes that following raw material forms according to parts by weight：
15-25 parts of sawdust, 5-8 parts of ginkgo leaf, 2-5 parts of analysis for soybean powder, oily 3-6 parts of bran, 2-6 parts of oyster shell whiting, potassium dihydrogen phosphate 0.3-1
Part, 0.3-1 parts of magnesium sulfate, 1-3 parts of peptone powder, 1-3 parts of agar.
Further, include that following raw material forms according to parts by weight：
120 parts of sawdust, 7 parts of ginkgo leaf, 4 parts of analysis for soybean powder, oily 5 parts of bran, 4 parts of oyster shell whiting, 0.6 part of potassium dihydrogen phosphate, magnesium sulfate 0.7
Part, 2 parts of peptone powder, 2 parts of agar.
Further, the sawdust is at least one of pine tree sawdust, yellow fruit trees bits.
Further, the oily bran is at least one of rapeseed oil bran, oil tea oil bran.
Further, the oyster shell whiting passes through 560 ~ 575 DEG C, and the internal environment calcining 1 of 0.03 ~ 0.08 atmospheric pressure ~
3h, is then polished into the sodium hydroxide solution that 10-15% is sprayed after powder, and 3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphoric acid tune
PH value is saved to 6-6.5.By the oyster shell whiting of the process, inventor is surprised to find that it has the growth of oil tea mushroom
Facilitation.
Further, the sawdust passes through the solarization -1 day water logging of immersion -5-10 days of the sky and water of solarization in 10-15 days -1
The solarization of bubble -1-3 days obtains sawdust.The treatment process can make the internal structure of sawdust more loose, be given birth to convenient for oil tea mushroom silk
At.
Further, which adjusts the pH7-7.2 of culture medium in process for preparation using pH adjusting agent.It is more conducively oily
The growth and breeding of tea mushroom improves the yield of oil tea mushroom.
Further, the pH adjusting agent is at least one of acetic acid, citric acid.Can both it be increased using the conditioning agent
Add the nutrition of culture medium, and the chlorine element in culture medium can be reduced, more conducively the growth and breeding of oil tea mushroom, improves oil tea mushroom
Yield.
Further, the sawdust, ginkgo leaf, analysis for soybean powder, oily bran are through high-pressure steam sterilizing pan sterilizing 1h or more, sterilizing
Temperature is 100 DEG C -110 DEG C.
The present invention technique effect be：Oil tea mushroom is planted using the culture medium that the present invention makes, at raw material sawdust
Reason, structure is more loose, and ventilation is preferable so that and oil tea mushroom is easier to decompose nutritional ingredient therein, meanwhile, the ginkgo leaf of addition
In more GINKGO BILOBA EXTRACT and lactones, coordinate the oyster shell whiting and other raw materials after specially treated, be greatly improved oil tea mushroom
Yield is improved, can guarantee that the growth of oil tea mushroom is vigorous, the body of rod of bacterial strain is not only sturdy but also length is more than common oil tea mushroom.
Specific implementation mode
Be described further below technical scheme of the present invention, but claimed range be not limited to it is described.
Embodiment one
Present embodiments provide a kind of oil tea mushroom culture medium, including following raw material composition：Sawdust 25kg, ginkgo leaf 8kg, analysis for soybean powder
5kg, oily bran 6kg, oyster shell whiting 6kg, potassium dihydrogen phosphate 1kg, magnesium sulfate 1kg, peptone powder 3kg, agar 3kg.
Sawdust is at least one of pine tree sawdust, yellow fruit trees bits among the above, and the present embodiment selects trees bits；Sawdust passes through
It crosses the solarization -1 of the immersion -5-10 days sky and water solarization of immersion -1-3 days of the sky and water of solarization in 10-15 days -1 and obtains sawdust.
Oily bran is at least one of rapeseed oil bran, oil tea oil bran, and the present embodiment selects rapeseed oil bran.
Above-mentioned oyster shell whiting passes through 560 ~ 575 DEG C, and the internal environment of 0.03 ~ 0.08 atmospheric pressure calcines 1 ~ 3h, then beats
Spray the sodium hydroxide solution of 10-15% after grinds, and 3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphorus acid for adjusting pH value to 6-
6.5.By the oyster shell whiting of the process, inventor is surprised to find that it has facilitation to the growth of oil tea mushroom.
The sawdust of the culture medium, ginkgo leaf, analysis for soybean powder, oily bran sterilize 1h or more through high-pressure steam sterilizing pan, sterilising temp
It is 100 DEG C -110 DEG C.Then all raw materials are crushed and is proportionally mixed, pH adjusting agent is used in process for preparation
Adjust the pH7-7.2 of culture medium.PH adjusting agent is at least one of acetic acid, citric acid, and the present embodiment selects acetic acid.
Embodiment two
Present embodiments provide a kind of oil tea mushroom culture medium, including following raw material composition：Sawdust 15kg, ginkgo leaf 5kg, analysis for soybean powder
2kg, oily bran 3kg, oyster shell whiting 2kg, potassium dihydrogen phosphate 0.3kg, magnesium sulfate 0.3kg, peptone powder 1kg, agar 1kg.
Sawdust is at least one of pine tree sawdust, yellow fruit trees bits among the above, and the present embodiment selects trees bits；Sawdust passes through
It crosses the solarization -1 of the immersion -5-10 days sky and water solarization of immersion -1-3 days of the sky and water of solarization in 10-15 days -1 and obtains sawdust.
Oily bran is at least one of rapeseed oil bran, oil tea oil bran, and the present embodiment selects rapeseed oil bran.
Above-mentioned oyster shell whiting passes through 560 ~ 575 DEG C, and the internal environment of 0.03 ~ 0.08 atmospheric pressure calcines 1 ~ 3h, then beats
Spray the sodium hydroxide solution of 10-15% after grinds, and 3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphorus acid for adjusting pH value to 6-
6.5.By the oyster shell whiting of the process, inventor is surprised to find that it has facilitation to the growth of oil tea mushroom.
The sawdust of the culture medium, ginkgo leaf, analysis for soybean powder, oily bran sterilize 1h or more through high-pressure steam sterilizing pan, sterilising temp
It is 100 DEG C -110 DEG C.Then all raw materials are crushed and is proportionally mixed, pH adjusting agent is used in process for preparation
Adjust the pH7-7.2 of culture medium.PH adjusting agent is at least one of acetic acid, citric acid, and the present embodiment selects citric acid.
Embodiment three
Present embodiments provide a kind of oil tea mushroom culture medium, including following raw material composition：Sawdust 120kg, ginkgo leaf 7kg, soya bean
Powder 4kg, oily bran 5kg, oyster shell whiting 4kg, potassium dihydrogen phosphate 0.6kg, magnesium sulfate 0.7kg, peptone powder 2kg, agar 2kg.
Sawdust is at least one of pine tree sawdust, yellow fruit trees bits among the above, and the present embodiment selects trees bits；Sawdust passes through
It crosses the solarization -1 of the immersion -5-10 days sky and water solarization of immersion -1-3 days of the sky and water of solarization in 10-15 days -1 and obtains sawdust.
Oily bran is at least one of rapeseed oil bran, oil tea oil bran, and the present embodiment selects rapeseed oil bran.
Above-mentioned oyster shell whiting passes through 560 ~ 575 DEG C, and the internal environment of 0.03 ~ 0.08 atmospheric pressure calcines 1 ~ 3h, then beats
Spray the sodium hydroxide solution of 10-15% after grinds, and 3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphorus acid for adjusting pH value to 6-
6.5.By the oyster shell whiting of the process, inventor is surprised to find that it has facilitation to the growth of oil tea mushroom.
The sawdust of the culture medium, ginkgo leaf, analysis for soybean powder, oily bran sterilize 1h or more through high-pressure steam sterilizing pan, sterilising temp
It is 100 DEG C -110 DEG C.Then all raw materials are crushed and is proportionally mixed, pH adjusting agent is used in process for preparation
Adjust the pH7-7.2 of culture medium.PH adjusting agent is at least one of acetic acid, citric acid, and the present embodiment selects citric acid.
In order to prove the technique effect of the present invention, the present invention also provides following tests examples：
Experimental group：The culture medium loading 10 made respectively using one~embodiment three is implemented（Diameter）*35（Length）Polypropylene Bag
And oil tea mushroom strains are inoculated with, respectively marked as A1, A2, A3.
Control group：It is packed into 10 using the patent CN201711318955.1 of the background technology culture mediums made（Diameter）*35
（Length）Polypropylene Bag is simultaneously inoculated with oil tea mushroom strains, marked as B1, uses the patent CN201410556228.9 systems of background technology
The culture medium of work is packed into 10（Diameter）*35（Length）Polypropylene Bag is simultaneously inoculated with oil tea mushroom strains, marked as B2.Selection example three
Formula, but be the absence of oyster shell whiting and ginkgo leaf, be packed into 10（Diameter）*35（Length）Polypropylene Bag is simultaneously inoculated with oil tea mushroom strains,
Marked as B3.
Above-mentioned culture medium is placed in the same greenhouse of culture medium, and black out culture is carried out in similar 25 degrees Celsius of culturing room,
Scribing line in every 3 days measures mycelial growth rate, each matrix selects 5 bags of observation mycelia growing states.It is the average life of statistics below
Long speed：
Table 1:Mycelial growth rate
| Group | Mycelial growth rate（mm） | The strong degree of mycelia |
| A1 | 6.45 | +++ |
| A2 | 6.56 | +++ |
| A3 | 6.49 | ++++ |
| B1 | 4.14 | ++ |
| B2 | 4.21 | ++ |
| B3 | 4.78 | + |
By above table statistics it is recognised that culture medium provided by the invention, can obviously accelerate the speed of mycelia growth, lead to
A3 is crossed compared with B3, it was demonstrated that the oyster shell whiting and ginkgo leaf and oyster shell whiting that the present invention adds play unexpected technique effect,
Meanwhile first oil tea mushroom is dried, it detects its protein and total amino acid content is detected, testing result is as follows：
Table 2：The protein and total amino acid content of oil tea mushroom
| Group | Protein（%） | Total amino acid content（%） |
| A1 | 21.5 | 17.4 |
| A2 | 21.7 | 17.3 |
| A3 | 21.9 | 17.5 |
| B1 | 18.5 | 15.1 |
| B2 | 17.6 | 14.7 |
| B3 | 17.5 | 14.8 |
It is counted by above table it is recognised that culture medium provided by the invention, can obviously increase protein and total amino acid
Content, by A3 compared with B3, it was demonstrated that the oyster shell whiting and ginkgo leaf and oyster shell whiting that the present invention adds play unexpected skill
Art effect.
Claims (9)
1. a kind of oil tea mushroom culture medium, feature exist：Include following raw material composition according to parts by weight：
15-25 parts of sawdust, 5-8 parts of ginkgo leaf, 2-5 parts of analysis for soybean powder, oily 3-6 parts of bran, 2-6 parts of oyster shell whiting, potassium dihydrogen phosphate 0.3-1
Part, 0.3-1 parts of magnesium sulfate, 1-3 parts of peptone powder, 1-3 parts of agar.
2. oil tea mushroom culture medium as described in claim 1, feature exist：Include following raw material composition according to parts by weight：
120 parts of sawdust, 7 parts of ginkgo leaf, 4 parts of analysis for soybean powder, oily 5 parts of bran, 4 parts of oyster shell whiting, 0.6 part of potassium dihydrogen phosphate, magnesium sulfate 0.7
Part, 2 parts of peptone powder, 2 parts of agar.
3. oil tea mushroom culture medium as claimed in claim 1 or 2, feature exist：The sawdust is pine tree sawdust, yellow fruit trees
At least one of bits.
4. oil tea mushroom culture medium as claimed in claim 1 or 2, feature exist：The oily bran is rapeseed oil bran, oil tea oil bran
At least one of.
5. oil tea mushroom culture medium as claimed in claim 1 or 2, feature exist：The oyster shell whiting passes through 560 ~ 575 DEG C,
The internal environment of 0.03 ~ 0.08 atmospheric pressure calcines 1 ~ 3h, is then polished into the sodium hydroxide solution of sprinkling 10-15% after powder, and
3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphorus acid for adjusting pH value to 6-6.5.
6. oil tea mushroom culture medium as claimed in claim 1 or 2, feature exist：The sawdust passes through 10-15 days solarization -1
The solarization of immersion -1-3 days of the solarization -1 of the immersion -5-10 days sky and water, the sky and water obtains sawdust.
7. oil tea mushroom culture medium as claimed in claim 1 or 2, feature exist：The culture medium uses pH tune in process for preparation
Save the pH7-7.2 that agent adjusts culture medium.
8. oil tea mushroom culture medium as claimed in claim 7, feature exist：The pH adjusting agent is in acetic acid, citric acid
It is at least one.
9. oil tea mushroom culture medium as claimed in claim 1 or 2, feature exist：The sawdust, ginkgo leaf, analysis for soybean powder, oily bran
Through high-pressure steam sterilizing pan sterilizing 1h or more, sterilising temp is 100 DEG C -110 DEG C.
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| CN201810631228.9A CN108575564A (en) | 2018-06-19 | 2018-06-19 | a kind of oil tea mushroom culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109566265A (en) * | 2019-01-22 | 2019-04-05 | 贵州省贵福菌业发展有限公司 | A kind of oil tea mushroom bag industrial production method |
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| CN101844946A (en) * | 2010-02-10 | 2010-09-29 | 李瑞菊 | Culture medium for planting pholiota adipose and preparation method thereof |
| CN103304341A (en) * | 2013-07-16 | 2013-09-18 | 王龙 | Agrocybe cylindracea culture medium and preparation method thereof |
| CN104311329A (en) * | 2014-10-20 | 2015-01-28 | 中山安荞生物科技有限公司 | Culture medium capable of increasing nutrient content of agrocybe cylindracea |
| CN105238699A (en) * | 2015-09-16 | 2016-01-13 | 江苏华绿生物科技股份有限公司 | Preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit |
| CN106366746A (en) * | 2016-08-22 | 2017-02-01 | 谢裕梦 | Making technology of shell powder |
| CN107628877A (en) * | 2017-10-27 | 2018-01-26 | 广西浙缘农业科技有限公司 | A kind of Agrocybe cylindracea culture medium containing Composite nutrient soil and preparation method thereof |
-
2018
- 2018-06-19 CN CN201810631228.9A patent/CN108575564A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101844946A (en) * | 2010-02-10 | 2010-09-29 | 李瑞菊 | Culture medium for planting pholiota adipose and preparation method thereof |
| CN103304341A (en) * | 2013-07-16 | 2013-09-18 | 王龙 | Agrocybe cylindracea culture medium and preparation method thereof |
| CN104311329A (en) * | 2014-10-20 | 2015-01-28 | 中山安荞生物科技有限公司 | Culture medium capable of increasing nutrient content of agrocybe cylindracea |
| CN105238699A (en) * | 2015-09-16 | 2016-01-13 | 江苏华绿生物科技股份有限公司 | Preparation method of liquid fermentation spawns for industrial cultivation of agrocybe aegirit |
| CN106366746A (en) * | 2016-08-22 | 2017-02-01 | 谢裕梦 | Making technology of shell powder |
| CN107628877A (en) * | 2017-10-27 | 2018-01-26 | 广西浙缘农业科技有限公司 | A kind of Agrocybe cylindracea culture medium containing Composite nutrient soil and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109566265A (en) * | 2019-01-22 | 2019-04-05 | 贵州省贵福菌业发展有限公司 | A kind of oil tea mushroom bag industrial production method |
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