CN107484554A - A kind of artificial collybia albuminosa cultural method - Google Patents
A kind of artificial collybia albuminosa cultural method Download PDFInfo
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- CN107484554A CN107484554A CN201710908409.7A CN201710908409A CN107484554A CN 107484554 A CN107484554 A CN 107484554A CN 201710908409 A CN201710908409 A CN 201710908409A CN 107484554 A CN107484554 A CN 107484554A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The present invention relates to fungus growing technique field, more particularly to a kind of cultural method of artificial collybia albuminosa.Present invention uses the raw materials such as Jujun grasses, bean dregs, peptone, carbonic acid, phosphoric acid, glucose, fructose, vitamin A, hollow corncob to replace conventional termite, termitarium associated extraction thing, and simulate the growing environment of field collybia albuminosa, without using termite, termitarium associated extraction thing, collybia albuminosa cultivating rate and yield are improved, the production cost and culture raw material for reducing collybia albuminosa obtain difficulty.
Description
Technical field
The present invention relates to fungus growing technique field, especially a kind of artificial collybia albuminosa cultural method.
Background technology
Collybia albuminosa is one of four big bacterium, is treasure in edible wild bacterium.Collybia albuminosa meat thickness is big and fleshy, and matter filament is white, taste
The fresh and sweet delicious and crisp in road.Containing amino acid, protein, fat necessary to human body, also containing various vitamins and calcium, phosphorus, core yellow acid etc.
Material.Collybia albuminosa can thin material for dish, can also be arranged in pairs or groups with vegetables, the flesh of fish and various all kinds of delicacies, no matter fry, fry, salting down, decocting, mixing,
It is braised, roasting, stewing, steam in clear soup or cook soup, its flavour is all very fresh, is the hat in bacterium.Some of collybia albuminosa only southwest, a few provinces in the southeast and Taiwan
Area produces, and because of its wild yields poorly, price is higher.
Collybia albuminosa is not only with abundant nutritive value, while also has very high medical value, with beneficial stomach, clearly
God, the effect such as hemorrhoid and reducing blood lipid is controlled, also with enriching the blood to restore normal menstruation, strengthening the spleen and stomach and other effects, available for treatment poor appetite, chronic diarrhea not
Only, all diseases of blood under hemorrhoid, it is one of traditional medicinal fungi in China.
However, in recent years, because ecological environment is continuous worsening, a large amount of administrations of soil planting process Pesticides, particularly
The mankind excavate under the driving of interests to the destructiveness of wild collybia albuminosa so that and the yield and quality of collybia albuminosa all decline to a great extent,
Price is constantly soaring, so as to exacerbate randomness excavation, forms vicious circle.
The growth mechanism of collybia albuminosa and most wild mushrooms has huge difference, and physiological property is very special, and collybia albuminosa exists
Gradual and termite enters to turn to the symbiosis ecosystem in long-term evolutionary process, and collybia albuminosa is dependent on the termitarium that termite is its construction
Fungus garden, the termite collybia albuminosa provide the multiple nutritional components of its needs.
At present, manually culture is typically to use medium culture to collybia albuminosa, transfers to the method planted in soil,
Such as CN104387138A (method of the production of culture bag and artificial growth collybia albuminosa of plantation collybia albuminosa), such method is due to chicken fir
Bacterium is high to environmental requirement, causes the nutritional character in compost to be difficult to fruiting when not meeting its characteristic requirements, that is, allows into
Work(fruiting, it is also desirable to which a large amount of soil for meeting collybia albuminosa growth requirement could be cultivated, and cause collybia albuminosa cultivation to be difficult to advise greatly
Mould is carried out, and yield is difficult to improve, and in order to increase collybia albuminosa cultivating rate and yield, is added generally in collybia albuminosa growth course white
Ant, termitarium associated extraction thing, such as CN105474995A (a kind of cultivation domestication method of wild collybia albuminosa), but it is termite, white
The acquisition of ant nest associated extraction thing is more difficult, adds production cost, constrains the development of collybia albuminosa industry.
The content of the invention
In order to solve above-mentioned technical problem present in prior art, the present invention provides a kind of artificial collybia albuminosa cultivation side
Method, it is achieved particular by following technical scheme：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 22-26 DEG C incubated, until mycelia covers with culture medium；
(4) according to parts by weight, Jujun grasses 35-45 parts, bean dregs 45-50 parts, peptone 10-12 parts, glucose 4-8 are weighed
Part, fructose 6-10 parts, -2 parts of retinol1, water 25-35 parts, it is well mixed after smashing, ph is adjusted with carbonic acid, phosphoric acid mixed solution
To 6-6.5, collybia albuminosa growth base is obtained, growth base is distributed into bag, every bag of growth base center is put into a hollow corncob, beautiful
Filled up in meter Xin with growth base, make the environment in growth base bag closer in termitarium surrounding environment, collybia albuminosa growth preferably；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 23-25 DEG C of indoor temperature of control, humidity 89-93%, 5-6 DEG C of day and night temperature, protected
Indoor dark culturing 50-55d is held, is that mycelia is sufficiently grown, improves cultivating rate；
(6) Jujun grasses 15-18 parts, bean dregs 15-18 parts, peptone 8-10 parts, glucose 6-10 parts are weighed, is stirred after smashing
Uniformly, growth base bag surface is covered in, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, 27-29 DEG C of environment temperature of control, air humidity 86-88%, day and night temperature are at 8-10 DEG C, in the daytime
In 150-200Lux, night keeps dark for intensity of illumination control, makes environment closer in the natural growing environment of collybia albuminosa, until
Harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, its composition are calculated as by weight, peeled potatoes slurry 120-160 parts, glucose 14-18
Part, fructose 4-6 parts, agar 8-12 parts, -4 parts of 3-Hydroxyretinol, peptone 8-14 parts, albumen powder 15-20 parts.
The albumen powder, it is soyabean protein powder.
It is preferred that the albumen powder, wherein containing soybean protein 65-78%, lactalbumin 22-35%.
The culture medium, it is also added into 0.04-0.1% protease.
The step (4), is first to weigh Jujun grasses, bean dregs, peptone, water, is smashed after well mixed, then with glucose,
Fructose, vitamin A fully mix, then adjust ph to 6-6.5 with carbonic acid, phosphoric acid mixed solution, collybia albuminosa growth base are obtained, by life
Long base is distributed into bag, and every bag of growth base center is put into a hollow corncob, filled up in corncob with growth base.
The step (4), all components weighed, by ultraviolet radiation sterilization.
The step (4), it is to dispense growth base according to 3.5-5kg/ bags.
The step (7), 10-15% albumen powder solution 40-50ml is added into every bag of growth base daily.Ensure chicken fir
Nutrient is sufficient during bacteria growing.
The step (7), in albumen powder used, various albumen are calculated as by weight, soybean protein 30-36 parts, whey egg
White 18-22 parts, Fish protein 8-10 parts, animal blood albumen 5-8 parts.Make wherein amino acid ligand ratio closer in termite nest bacterial nursery.
Compared with prior art, the technique effect of the invention is embodied in：
Present invention uses Jujun grasses, bean dregs, peptone, carbonic acid, phosphoric acid, glucose, fructose, vitamin A, hollow corn
The raw materials such as core replace conventional termite, termitarium associated extraction thing, and simulate the growing environment of field collybia albuminosa, without using white
Ant, termitarium associated extraction thing, improve collybia albuminosa cultivating rate and yield, reduce the production cost and culture raw material of collybia albuminosa
Obtain difficulty.
Embodiment
Limited with reference to specific embodiment technical scheme is further, but claimed
Scope is not only limited to made description.
Embodiment 1
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 24 DEG C incubated, until mycelia covers with culture medium；
(4) Jujun grasses 40kg, bean dregs 47.5kg, peptone 11kg, glucose 6kg, fructose 8kg, vitamin are weighed
A1.5kg, water 30kg, it is well mixed after smashing, ph to 6.2 is adjusted with carbonic acid, phosphoric acid mixed solution, obtains collybia albuminosa growth base,
Base will be grown according to 4.25kg/ bags and is distributed into bag, and every bag of growth base center is put into a hollow corncob, growth is used in corncob
Base fills up, and makes the environment in growth base bag closer in termitarium surrounding environment, collybia albuminosa growth preferably；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 4 DEG C of indoor temperature of control, humidity 91%, 5.5 DEG C of day and night temperature, keep indoor black
Light culture 52.5d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) Jujun grasses 16.5kg, bean dregs 16.5kg, peptone 9kg, glucose 8kg are weighed, stirs, covers after smashing
Cover on growth base bag surface, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 28 DEG C of environment temperature, air humidity 87%, day and night temperature at 9 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to remove skin potato pulp 140g, glucose 16g, fructose 5g, agar 10g, vitamin
A3.5g, peptone 11g, soyabean protein powder 17.5g, are mixed.
Embodiment 2
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 22 DEG C incubated, until mycelia covers with culture medium；
(4) Jujun grasses 35kg, bean dregs 45kg, peptone 10kg, glucose 4kg, fructose 6kg, retinol1 kg, water are weighed
25kg, it is well mixed after smashing, ph to 6 is adjusted with carbonic acid, phosphoric acid mixed solution, collybia albuminosa growth base is obtained, according to 3.5kg/
Growth base is distributed into bag by bag, and every bag of growth base center is put into a hollow corncob, is filled up in corncob with growth base, make life
Environment in long base bag is closer in termitarium surrounding environment, collybia albuminosa growth preferably；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 23 DEG C of indoor temperature of control, humidity 89%, 5 DEG C of day and night temperature, keep indoor black
Light culture 50d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) 15 parts of Jujun grasses, 15 parts of bean dregs, 8 parts of peptone, 6 parts of glucose are weighed, stirs, is covered in after smashing
Base bag surface is grown, thickness 0.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 27 DEG C of environment temperature, air humidity 86%, day and night temperature at 8 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to remove skin potato pulp 120g, glucose 14g, fructose 4g, agar 8g, vitamin
A3g, peptone 8g, soyabean protein powder 15g, are mixed.
Embodiment 3
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 26 DEG C incubated, until mycelia covers with culture medium；
(4) weigh Jujun grasses 45kg, bean dregs 50kg, peptone 12kg, glucose 8kg, fructose 10kg, dehydroretinol kg,
Water 35kg, it is well mixed after smashing, ph to 6.5 is adjusted with carbonic acid, phosphoric acid mixed solution, obtains collybia albuminosa growth base, according to
5kg/ bags are distributed into bag by base is grown, and every bag of growth base center is put into a hollow corncob, filled up in corncob with growth base,
Make the environment in growth base bag closer in termitarium surrounding environment, collybia albuminosa growth preferably；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 25 DEG C of indoor temperature of control, humidity 93%, 6 DEG C of day and night temperature, keep indoor black
Light culture 55d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) according to weight kg, Jujun grasses 18kg, bean dregs 18kg, peptone 10kg, glucose 10kg is weighed, is stirred after smashing
Mix uniformly, be covered in growth base bag surface, thickness 1.5cm, the nutrient being consumed in base grown with supplement；
(7) continue to cultivate, control 29 DEG C of environment temperature, air humidity 88%, day and night temperature at 10 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to remove skin potato pulp 160g, glucose 18g, fructose 6g, agar 12g, vitamin
A4g, peptone 14g, soyabean protein powder 20g, are mixed.
Embodiment 4
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 24 DEG C incubated, until mycelia covers with culture medium；
(4) Jujun grasses 40kg, bean dregs 47.5kg, peptone 11kg, glucose 6kg, fructose 8kg, vitamin are weighed
A1.5kg, water 30kg, it is well mixed after smashing, ph to 6.2 is adjusted with carbonic acid, phosphoric acid mixed solution, obtains collybia albuminosa growth base,
Base will be grown according to 4.25kg/ bags and is distributed into bag, and every bag of growth base center is put into a hollow corncob, growth is used in corncob
Base fills up, and makes the environment in growth base bag closer in termitarium surrounding environment, collybia albuminosa growth preferably；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 4 DEG C of indoor temperature of control, humidity 91%, 5.5 DEG C of day and night temperature, keep indoor black
Light culture 52.5d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) Jujun grasses 16.5kg, bean dregs 16.5kg, peptone 9kg, glucose 8kg are weighed, stirs, covers after smashing
Cover on growth base bag surface, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 28 DEG C of environment temperature, air humidity 87%, day and night temperature at 9 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to remove skin potato pulp 140g, glucose 16g, fructose 5g, agar 10g, vitamin
A3.5g, peptone 11g, albumen powder 17.5g, are mixed.Albumen powder used contains soybean protein 71.5%, lactalbumin
28.5%.
Embodiment 5
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 24 DEG C incubated, until mycelia covers with culture medium；
(4) Jujun grasses 40kg, bean dregs 47.5kg, peptone 11kg, glucose 6kg, fructose 8kg, vitamin are weighed
A1.5kg, water 30kg, it is well mixed after smashing, ph to 6.2 is adjusted with carbonic acid, phosphoric acid mixed solution, obtains collybia albuminosa growth base,
Base will be grown according to 4.25kg/ bags and is distributed into bag, and every bag of growth base center is put into a hollow corncob, growth is used in corncob
Base fills up, and makes the environment in growth base bag closer in termitarium surrounding environment, collybia albuminosa growth preferably；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 4 DEG C of indoor temperature of control, humidity 91%, 5.5 DEG C of day and night temperature, keep indoor black
Light culture 52.5d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) Jujun grasses 16.5kg, bean dregs 16.5kg, peptone 9kg, glucose 8kg are weighed, stirs, covers after smashing
Cover on growth base bag surface, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 28 DEG C of environment temperature, air humidity 87%, day and night temperature at 9 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to remove skin potato pulp 140g, glucose 16g, fructose 5g, agar 10g, vitamin
A3.5g, peptone 11g, soyabean protein powder 17.5g, 0.07% protease is added after mixing, is produced.
Embodiment 6
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 24 DEG C incubated, until mycelia covers with culture medium；
(4) Jujun grasses 40kg, bean dregs 47.5kg, peptone 11kg, water 30kg are first weighed, is smashed after well mixed, then is added
Enter glucose 6kg, fructose 8kg, retinol1 .5kg to be well mixed, adjust ph to 6.2 with carbonic acid, phosphoric acid mixed solution, obtain
Collybia albuminosa grows base, and bag is distributed into by base is grown according to 4.25kg/ bags, and every bag of growth base center is put into a hollow corncob,
Filled up in corncob with growth base, make the environment in growth base bag closer in termitarium surrounding environment, collybia albuminosa life preferably
It is long；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 4 DEG C of indoor temperature of control, humidity 91%, 5.5 DEG C of day and night temperature, keep indoor black
Light culture 52.5d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) Jujun grasses 16.5kg, bean dregs 16.5kg, peptone 9kg, glucose 8kg are weighed, stirs, covers after smashing
Cover on growth base bag surface, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 28 DEG C of environment temperature, air humidity 87%, day and night temperature at 9 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to remove skin potato pulp 140g, glucose 16g, fructose 5g, agar 10g, vitamin
A3.5g, peptone 11g, soyabean protein powder 17.5g, are mixed.
Embodiment 7
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 24 DEG C incubated, until mycelia covers with culture medium；
(4) Jujun grasses 40kg, bean dregs 47.5kg, peptone 11kg, glucose 6kg, fructose 8kg, vitamin are weighed
A1.5kg, water 30kg, sterilizing is irradiated using ultraviolet, then smashes and is well mixed, adjusted with carbonic acid, phosphoric acid mixed solution
Ph to 6.2 is saved, obtains collybia albuminosa growth base, is distributed into bag by base is grown according to 4.25kg/ bags, every bag of growth base center is put into one
Individual hollow corncob, filled up with growth base in corncob, make environment in growth base bag closer in termitarium surrounding environment, more
Suitable collybia albuminosa growth；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 4 DEG C of indoor temperature of control, humidity 91%, 5.5 DEG C of day and night temperature, keep indoor black
Light culture 52.5d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) Jujun grasses 16.5kg, bean dregs 16.5kg, peptone 9kg, glucose 8kg are weighed, stirs, covers after smashing
Cover on growth base bag surface, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 28 DEG C of environment temperature, air humidity 87%, day and night temperature at 9 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to remove skin potato pulp 140g, glucose 16g, fructose 5g, agar 10g, vitamin
A3.5g, peptone 11g, soyabean protein powder 17.5g, are mixed.
Embodiment 8
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 24 DEG C incubated, until mycelia covers with culture medium；
(4) Jujun grasses 40kg, bean dregs 47.5kg, peptone 11kg, glucose 6kg, fructose 8kg, vitamin are weighed
A1.5kg, water 30kg, it is well mixed after smashing, ph to 6.2 is adjusted with carbonic acid, phosphoric acid mixed solution, obtains collybia albuminosa growth base,
Base will be grown according to 4.25kg/ bags and is distributed into bag, and every bag of growth base center is put into a hollow corncob, growth is used in corncob
Base fills up, and makes the environment in growth base bag closer in termitarium surrounding environment, collybia albuminosa growth preferably；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 4 DEG C of indoor temperature of control, humidity 91%, 5.5 DEG C of day and night temperature, keep indoor black
Light culture 52.5d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) Jujun grasses 16.5kg, bean dregs 16.5kg, peptone 9kg, glucose 8kg are weighed, stirs, covers after smashing
Cover on growth base bag surface, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 28 DEG C of environment temperature, air humidity 87%, day and night temperature at 9 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, adds 12.5% albumen powder solution 45ml into every bag of growth base daily, makes
Environment is closer in the natural growing environment of collybia albuminosa, until harvesting, in albumen powder used, various albumen are calculated as by weight,
33 parts of soybean protein, 20 parts of lactalbumin, 9 parts of Fish protein, 6.5 parts of animal blood albumen；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to remove skin potato pulp 140g, glucose 16g, fructose 5g, agar 10g, vitamin
A3.5g, peptone 11g, soyabean protein powder 17.5g, are mixed.
Comparative example 1
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 24 DEG C incubated, until mycelia covers with culture medium；
(4) maize cob meal 100kg, coarse cereal powder 17.5kg, sucrose 2.75kg, amino acid 2.75kg, sodium formate are weighed
2.5kg, land plaster 2.75kg, water 15kg, it is well mixed, obtains collybia albuminosa growth base, will grows base packing according to 4.25kg/ bags
Into bag；
(5) growth base bag is cultivated to growing in base bag, controls indoor temperature by the mycelium inoculation in culture medium indoors
4 DEG C, humidity 91%, 5.5 DEG C of day and night temperature, keep indoor dark culturing 52.5d, be that mycelia is sufficiently grown, improve out
Mushroom rate；
(6) Jujun grasses 16.5kg, bean dregs 16.5kg, peptone 9kg, glucose 8kg are weighed, stirs, covers after smashing
Cover on growth base bag surface, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 28 DEG C of environment temperature, air humidity 87%, day and night temperature at 9 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, is the beans 100g that fetches earth, glucose 15g, agar 15g, peptone 7.5g, calcium lactate 3g,
KH2PO43g, water 1000g, is mixed.
Compared with embodiment 1, this comparative example employs conventional collybia albuminosa culture medium and growth base.
Comparative example 2
A kind of artificial collybia albuminosa cultural method：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 24 DEG C incubated, until mycelia covers with culture medium；
(4) wood chip 25kg, soluble alcohol grain 22.5kg, insoluble alcohol grain 22.5kg, wheat bran 20kg, termite row are weighed
Product thing 10kg, water 120kg, are well mixed after smashing, and obtain collybia albuminosa growth base, are distributed into according to 4.25kg/ bags by base is grown
Bag；
(5) by the mycelium inoculation in culture medium to grow base bag in corncob above, beneficial to mycelia it is ventilated and inhale
Adopt point, growth base bag is cultivated indoors, 4 DEG C of indoor temperature of control, humidity 91%, 5.5 DEG C of day and night temperature, keep indoor black
Light culture 52.5d, it is that mycelia is sufficiently grown, improves cultivating rate；
(6) Jujun grasses 16.5kg, bean dregs 16.5kg, peptone 9kg, glucose 8kg are weighed, stirs, covers after smashing
Cover on growth base bag surface, thickness 0.5-1.5cm, the nutrient being consumed in base is grown with supplement；
(7) continue to cultivate, control 28 DEG C of environment temperature, air humidity 87%, day and night temperature at 9 DEG C, in the daytime intensity of illumination
In 150-200Lux, night keeps dark for control, makes environment closer in the natural growing environment of collybia albuminosa, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
The collybia albuminosa culture medium, it is to take potato 200g, glucose 20g, agar 20g, water 1000g, soluble alcohol
Poor 1240g, is mixed.
Compared with embodiment 1, this comparative example employs conventional collybia albuminosa culture medium, added with the growth of termite long-pending thing of row
Base.
Embodiment 1-8, comparative example 1-2 are contrasted：
| Cultivating rate (%) | Yield rate (%) | Yield (kg/ (mu *)) | |
| Embodiment 1 | 91.8 | 92.5 | 7800 |
| Embodiment 2 | 89.6 | 89.2 | 7400 |
| Embodiment 3 | 90.4 | 88.7 | 7600 |
| Embodiment 4 | 92.5 | 93.4 | 8200 |
| Embodiment 5 | 95.4 | 94.6 | 8800 |
| Embodiment 6 | 92.2 | 93.7 | 8600 |
| Embodiment 7 | 92.0 | 89.9 | 8100 |
| Embodiment 8 | 92.2 | 97.6 | 9300 |
| Comparative example 1 | 82.1 | 78.6 | 5400 |
| Comparative example 2 | 93.4 | 92.8 | 8200 |
Embodiment 1 is significantly better than that using conventional collybia albuminosa culture medium and growth in yield, cultivating rate, yield rate etc.
The comparative example 1 of base, illustrate the present invention use culture medium, growth based formulas be more suitable for collybia albuminosa growth.With using conventional chicken
Fir bacterium culture medium is compared with the addition of the comparative example 1 of termite long-pending thing of row growth base, and embodiment 1 is in yield, cultivating rate, yield rate etc.
Aspect is slightly not so good as, but very nearly the same, illustrates the method for the present invention and can be trained instead of using termite, termitarium associated extraction thing
The method for supporting collybia albuminosa
Finally it is pointed out that above example is only the more representational example of the present invention.Obviously, technology of the invention
Scheme is not limited to above-described embodiment, can also there is many deformations.One of ordinary skill in the art can be from disclosed by the invention
All deformations that content is directly exported or associated, are considered as protection scope of the present invention.
Claims (10)
1. a kind of artificial collybia albuminosa cultural method, it is characterised in that comprise the following steps：
(1) the isolated mycelium from collybia albuminosa trophosome；
(2) mycelium is seeded in collybia albuminosa culture medium；
(3) by the culture medium after inoculation, 22-26 DEG C incubated, until mycelia covers with culture medium；
(4) according to parts by weight, Jujun grasses 35-45 parts, bean dregs 45-50 parts, peptone 10-12 parts, glucose 4-8 parts, fruit are weighed
Sugared 6-10 parts, -2 parts of retinol1, water 25-35 parts, it is well mixed after smashing, ph to 6- is adjusted with carbonic acid, phosphoric acid mixed solution
6.5, collybia albuminosa growth base is obtained, growth base is distributed into bag, every bag of growth base center is put into a hollow corncob, corncob
It is middle to be filled up with growth base；
(5) by above the mycelium inoculation in culture medium to the corncob grown in base bag, growth base bag is cultivated indoors, controlled
23-25 DEG C of indoor temperature, humidity 89-93%, 5-6 DEG C of day and night temperature, keep indoor dark culturing 50-55d；
(6) Jujun grasses 15-18 parts, bean dregs 15-18 parts, peptone 8-10 parts, glucose 6-10 parts are weighed, is stirred after smashing equal
It is even, it is covered in growth base bag surface, thickness 0.5-1.5cm；
(7) continue to cultivate, 27-29 DEG C of environment temperature of control, air humidity 86-88%, day and night temperature are at 8-10 DEG C, illumination in the daytime
For strength control in 150-200Lux, night keeps dark, until harvesting；
(8) when cap will stretch, when being not yet fully deployed, harvesting.
2. artificial collybia albuminosa cultural method according to claim 1, it is characterised in that the collybia albuminosa culture medium, its into
Divide and be calculated as by weight, peeled potatoes slurry 120-160 parts, glucose 14-18 parts, fructose 4-6 parts, agar 8-12 parts, dimension life
Plain A3-4 parts, peptone 8-14 parts, albumen powder 15-20 parts.
3. artificial collybia albuminosa cultural method according to claim 2, it is characterised in that the albumen powder, be soybean protein
Powder.
4. artificial collybia albuminosa cultural method according to claim 2, it is characterised in that the albumen powder, wherein containing big
Legumin 65-78%, lactalbumin 22-35%.
5. artificial collybia albuminosa cultural method according to claim 2, it is characterised in that the culture medium, be also added into
0.04-0.1% protease.
6. artificial collybia albuminosa cultural method according to claim 1, it is characterised in that the step (4), be first weigh it is huge
Juncao, bean dregs, peptone, water, smash after well mixed, then fully mixed with glucose, fructose, vitamin A, then with carbonic acid,
Phosphoric acid mixed solution adjusts ph to 6-6.5, obtains collybia albuminosa growth base, growth base is distributed into bag, every bag of growth base center is put
Enter a hollow corncob, filled up in corncob with growth base.
7. artificial collybia albuminosa cultural method according to claim 1, it is characterised in that the step (4), the institute weighed
There is component, by ultraviolet radiation sterilization.
8. artificial collybia albuminosa cultural method according to claim 1, it is characterised in that the step (4), is according to 3.5-
5kg/ bags dispense growth base.
9. artificial collybia albuminosa cultural method according to claim 1, it is characterised in that the step (7), daily to every bag
Grow the albumen powder solution 40-50ml that 10-15% is added in base.
10. artificial collybia albuminosa cultural method according to claim 9, it is characterised in that the step (7), albumen used
In powder, various albumen are calculated as by weight, soybean protein 30-36 parts, lactalbumin 18-22 parts, Fish protein 8-10 parts, animal blood
Albumen 5-8 parts.
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