CN103172446A - Fluid nutrient medium for large-scale cultivation of termitomyces albuminosus mycelium and application thereof - Google Patents
Fluid nutrient medium for large-scale cultivation of termitomyces albuminosus mycelium and application thereof Download PDFInfo
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- CN103172446A CN103172446A CN2013100986073A CN201310098607A CN103172446A CN 103172446 A CN103172446 A CN 103172446A CN 2013100986073 A CN2013100986073 A CN 2013100986073A CN 201310098607 A CN201310098607 A CN 201310098607A CN 103172446 A CN103172446 A CN 103172446A
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Abstract
The invention provides fluid nutrient medium for large-scale cultivation of termitomyces albuminosus mycelium and application thereof. The culture medium is prepared from the following materials in parts by weight: 50-70 parts of lignocellulose powder, 30-50 parts of water, 0.0001-0.1 part of monopotassium phosphate, 0.0001-0.1 part of sodium chloride, 0.0001-0.1 part of calcium chloride, 0.0001-0.1 part of cobalt chloride, 0.0001-0.1 part of magnesium sulfate, 0.0001-0.1 part of manganese sulfate, 0.0001-0.1 part of ferrous sulfate, 0.0001-0.1 part of copper sulfate, 0.0001-0.1 part of zinc sulfate, 0.0001-0.1 part of aluminum potassium sulfate, 0.0001-0.1 part of ammonium tartrate, 0.0001-0.1 part of boric acid, 0.0001-0.1 part of citric acid, 0.0001-0.1 part of formic acid, 0.0001-0.1 part of acetic acid, 0.0001-0.1 part of fumaric acid, 0.0001-0.1 part of succinic acid, 0.0001-0.1 part of propylene-1,2,3-tricarboxylic acid, and 0.0001-0.1 part of guaiacol. The termitomyces albuminosus of the fluid nutrient medium disclosed by the invention is customized, and can be used for large-scale indoor cultivation of termitomyces albuminosus mycelium by using lignocelluloses; and the fluid nutrient medium is low in cost and high in yield.
Description
(1) technical field
The present invention relates to a kind of liquid nutrient medium and application thereof for Collybia albuminosa mycelia mass-producing cultivation.
(2) background technology
Collybia albuminosa (Termitomyces albuminosus) is under the jurisdiction of Basidiomycotina Hymenomycetes Agaricales Tricholomataceae, is a kind of famous and precious wild edible fungus.The scientific research history of existing more than two hundred year of people to Collybia albuminosa, at first German natural scientist J.D.K ǒ ning in 1799 is finding on the fungus garden to give birth to many fungies that can form the jack shape in the termitarium of India eastern region, namely is known as afterwards the fungi of Collybia albuminosa.For termite, the mycelium of Collybia albuminosa is their Major Foods, and they are cultivated or the mycelial purpose of " cultivation " Collybia albuminosa also is this.The mycelia of Collybia albuminosa extensively distributes in whole ant nest, and when termite went to search for food, it was replaced by further growth again.Termite piles up on fungus garden nutrition as the Collybia albuminosa growth with indigested food, and the fungus garden that termite was infected take Collybia albuminosa is as food.
The research discovery, the fungus garden in termitarium is become to be grouped into other by half digest, wood fibre, soil particle, Collybia albuminosa mycelia, the worker ant secretory product of worker ant.According to analysis, contain protein 6.44%, fat 0.48%, total reducing sugar 0.24%, robust fibre 13.9%, ash content 26.6%, P in termite fugus gardens
2O
50.28%, Ca1.86%, K
2O1.56%, amino acid/11 8.7%.
Temperature-stable is at 22 ± 2 ℃ in termitarium, and minimum is 15 ℃, the highlyest is no more than 28 ℃.Stable temperature is grown to the Collybia albuminosa mycelial growth and has been created good condition.Relative air humidity in termitarium is 80%-90%.The fungus garden soil moisture content is 46.75%-58.35%.In addition, the respiratory metabolism activity of termite in nest has produced the carbonic acid gas of higher concentration, CO
2Concentration can reach 3-5%, the CO of high density
2More be conducive to the growth of Collybia albuminosa mycelia, and unfavorable to the growth of its sporophore and other fungi.Simultaneously, a large amount of formic acid of Released by Termites, acetic acid, propylene-1,2,3-tricarboxylic acid, FUMARIC ACID TECH GRADE, succinic acid etc. have been regulated the pH of fungus garden, make pH be stabilized in 4-4.5, this has also produced restraining effect to the growth of other fungus and bacteriums in fungus garden, makes Collybia albuminosa mycelia energy Fast Growth.
Due to Collybia albuminosa energy lignocellulose degradation, so it has a very important role in bioenergy industry.Yet although people have done large quantity research to the artificial culture of Collybia albuminosa Termitomyces albuminosus, knowing that Collybia albuminosa is cultivated under artificial condition needs certain CO
2Concentration, high humidity and acid condition, but up to now, because kind and the effects in the Collybia albuminosa growth and development process such as concentration, fungus garden symbiotic bacterium to plant composition, species of metal ion and concentration, acid also are short in understanding, also there is no a kind of lignocellulose that utilizes as the method for Major Nutrient base large scale culturing Collybia albuminosa mycelia.
(3) summary of the invention
In order to realize industrialized application, the invention provides a kind of in indoor liquid nutrient medium and the application thereof that utilizes lignocellulose large scale culturing Collybia albuminosa mycelia.
The technical solution used in the present invention is:
A kind of liquid nutrient medium for Collybia albuminosa mycelia mass-producing cultivation, prepare by following quality proportioning:
Described lignocellulose is 1: 1 mixture of white poplar wood powder and mugwort powder.White poplar Populus tomentosa Carr wood powder obtains from sawmill, and 10 mesh sieves that dried in the sun are standby; The mugwort powder was pulverized the dry powder of 10 mesh sieves for the mugwort Artemisia indica Willd plant of collecting from the field with pulverizer after drying under the sun.
In this formula, lignocellulose is grown for mycelial growth provides carbon source and energy matter, metallic salt is grown for mycelial growth provides essential metal ion, acid compounds is grown for mycelial growth provides essential sour environment, methyl catechol provides signal for the Laccase in the mycelial growth growth course, and water is grown for mycelial growth to be provided essential moisture content and keep essential humidity.
Preferably, described substratum is prepared by following quality proportioning:
More preferred, described substratum is prepared by following quality proportioning:
The invention still further relates to the application of described liquid nutrient medium in the mass-producing of Collybia albuminosa mycelia is cultivated.
Concrete, described being applied as: by the formulated liquid nutrient medium, add Collybia albuminosa bacterium liquid and fungal component liquid, cultivated 30 days under 12 ℃～30 ℃, obtain the Collybia albuminosa fermented liquid, fermented liquid after filtration, filtrate is Collybia albuminosa bacterium liquid, this bacterium liquid namely can be used for the degraded of lignocellulose and the commercialization production of Collybia albuminosa.Symbiotic bacterium liquid is for separating, cultivate the symbiotic bacterium that obtains from the blackwing subterranean fungus garden, these bacteriums comprise: 4 kinds of bacterium of Bacillus thuringiensis (Bacillus thuringiensis), bacillus (Bacillussp.) and clostridium bifermentans (Clostridium bifermentans) etc. are used for removing some harmful secondary compound that the mycelial growth growth course produces and the growth of growing and promote the Collybia albuminosa mycelia that suppresses other fungi.Collybia albuminosa bacterium liquid is the Collybia albuminosa mycelia aqueous solution that utilizes potato dextrose agar to cultivate, and cultivating for the mycelia mass-producing provides bacterial classification.
Beneficial effect of the present invention is mainly reflected in:
(1) directly use the powder mixture of various plants as the carbon source of Collybia albuminosa mycelium culture, cost is economized, and simultaneously, also can promote the utilization of nature and waste resource;
(2) in nutrient solution, add simultaneously the various metals salt compounds, for various enzymes in the mycelial growth growth course synthetic provides essential metal ion, make the Collybia albuminosa mycelia to grow by normal growth on the lignocellulose substratum;
(3) in nutrient solution, add simultaneously multiple acid compounds, on the one hand the growth for the Collybia albuminosa mycelia provides good sour environment, and such sour environment has limited the growth of other mushroom on the other hand, then provides information substance for the growth of Collybia albuminosa mycelia on the one hand;
(5) in nutrient solution, add methyl catechol, can induce Collybia albuminosa to produce laccase, promotion can destroy rapidly xylogen in the plant drymeal mixture as the Collybia albuminosa mycelia of bacterial classification, make the Collybia albuminosa mycelia can have that enough nutrition is grown, development and fecundity, thereby realize the quick production of Collybia albuminosa mycelia;
(4) add the symbiotic bacterium liquid that is consisted of by multiple fungus garden symbiotic bacterium in culturing process, the growth of the small insects such as fruit bat in the culturing process and the growth of other miscellaneous bacteria have been suppressed on the one hand, on the other hand symbiotic bacterium consumed in growth and development process produce in Collybia albuminosa mycelial growth growth course to harmful secondary compound that itself grows, make the Collybia albuminosa mycelia can normal growth and growth.
(4) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
Bacterial classification:
Take the blackwing subterranean nest from the field, fungus garden is taken back indoor.Moved into jack bacterium (vegetative phase of Collybia albuminosa) on slant medium (potato dextrose agar) same day, and good inclined-plane moves the pipe purifying to select growing way, and the bacterial classification after purifying gives over to female the kind;
The preparation of potato dextrose agar: peeling potato 200g, glucose 20g, agar 15～20g, distilled water 1000ml.To remove the peel potato and be cut into small pieces, water boil 30min with 8 layers of filtered through gauze, adds glucose and agar in filtrate, and water is settled to 1000ml, pours into after autoclaving to be used for spawn culture and purifying in Glass tubing.
The acquisition of Collybia albuminosa bacterium liquid: with potato dextrose agar and the upper Collybia albuminosa silk of cultivating thereof, the solid-liquid mass ratio according to 1:1 joins in sterilized water, fully stirs, and after standing 1 hour, suspension is poured out, and this suspension is Collybia albuminosa bacterium liquid.
The acquisition of fungal component liquid: the blackwing subterranean fungus garden that will dig from the field nest acquisition pulverizes with instrument, and then according to fungus garden: the mass ratio of sterilized water=1:9 mixes, and stirs, and standing 2 hours, gets supernatant liquor and is fungal component liquid.
Culture medium prescription is (mass parts):
Production stage is:
(1) accurately take various salts (potassium primary phosphate, sodium-chlor, calcium chloride, cobalt chloride, sal epsom, manganous sulfate, ferrous sulfate, copper sulfate, zinc sulfate, potassium aluminium sulfate, ammonium tartrate) with scale, then water dissolves it, be made into the metallic salt aqueous solution, standby.
(2) accurately take various acid (boric acid, citric acid, formic acid, acetic acid, FUMARIC ACID TECH GRADE, succinic acid, propylene-1,2,3-tricarboxylic acid) with scale, then it is dissolved in water, be made into acid solution, standby.
(3) accurately take symbiotic bacterium liquid with scale, it is put in bucket, standby.
(4) accurately take Termitomyces albuminosus solution and methyl catechol with scale, place it in bucket and stir, standby.
(5) accurately take lignocellulose with scale, place it in container, then add the metallic salt aqueous solution, stir; Add again acid solution, stir; Add again symbiotic bacterium liquid, stir; Add again the mixed solution of Collybia albuminosa and methyl catechol, stir; At last, will fill the container cover upper cover of said mixture, be placed in the environment of 12 ℃～30 ℃.
After (6) 30 days, remove the lid on container, the fermented product in container is filtered, filter residue is used for the further base-material of cultivating the Collybia albuminosa mycelia or cultivating as earthworm, cockroach, filtrate is Collybia albuminosa bacterium liquid, and this bacterium liquid namely can be used for the degraded of lignocellulose and the commercialization production of Collybia albuminosa.
Cultivate according to above method, not only can obtain to cultivate the low a large amount of mycelia stostes more than 20% of cost than potato dextrose agar, and can directly be used as the raw material of earthworm, cockroach cultivation through the wood powder that mycelia is processed, thereby provide a kind of new way of getting rich for hill farmer.Simultaneously, through the wood powder that Collybia albuminosa infects repeatedly, also can be used as the feed of ox, sheep, be used for ox, sheep cultivation.In addition, utilize this formula to cultivate, the mycelia amount of acquisition increases by 4500 times of left and right.
Embodiment 2:
Substratum quality formula is as follows, and other are with embodiment 1:
Utilize this formula to cultivate, the mycelia amount of acquisition increases by 3800 times of left and right.
Embodiment 3:
Substratum quality formula is as follows, and other are with embodiment 1:
Utilize this formula to cultivate, the mycelia amount of acquisition increases by 3000 times of left and right.
Embodiment 4:
Substratum quality formula is as follows, and other are with embodiment 1:
Utilize this formula to cultivate, the mycelia amount of acquisition increases by 3500 times of left and right.
Embodiment 5:
Substratum quality formula is as follows, and other are with embodiment 1:
Utilize this formula to cultivate, the mycelia amount of acquisition increases by 4000 times of left and right.
Claims (4)
1. one kind is used for the liquid nutrient medium that the mass-producing of Collybia albuminosa mycelia is cultivated, and prepares by following quality proportioning:
Described lignocellulose is 1: 1 mixture of white poplar wood powder and mugwort powder.
2. liquid nutrient medium as claimed in claim 1 is characterized in that described substratum prepares by following quality proportioning:
3. liquid nutrient medium as claimed in claim 1 is characterized in that described substratum prepares by following quality proportioning:
4. the application of liquid nutrient medium as described in one of claim 1～3 in the mass-producing of Collybia albuminosa mycelia is cultivated.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105462869A (en) * | 2014-08-07 | 2016-04-06 | 马前 | Clostridium bifermentans and uses thereof, and nanometer metal sulfide preparation method |
| CN105695338A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus |
| CN107400018A (en) * | 2017-09-18 | 2017-11-28 | 田林县群英农业有限公司 | A kind of cultivation fertilizer of collybia albuminosa and preparation method thereof |
| CN107484554A (en) * | 2017-09-29 | 2017-12-19 | 贵州棒棒食用菌产业有限公司 | A kind of artificial collybia albuminosa cultural method |
| CN107950303A (en) * | 2017-11-29 | 2018-04-24 | 四川山水美地农业投资有限公司 | A kind of cultural method of Termitomyces albuminosus with black skin parent species |
| CN109673379A (en) * | 2017-09-29 | 2019-04-26 | 贵州棒棒食用菌产业有限公司 | A kind of collybia albuminosa cultural method |
| CN110036987A (en) * | 2019-06-03 | 2019-07-23 | 昆明市林业和草原科学研究所 | Utilize the method for artificial culture ant nest fungus garden cultivation collybia albuminosa |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2579254C1 (en) * | 2015-04-21 | 2016-04-10 | Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт мелиорированных земель" (ФГБНУ ВНИИМЗ) | Method of producing biological fertiliser |
| CN107646515A (en) * | 2017-08-30 | 2018-02-02 | 山东省科创食用菌产业技术研究院 | A kind of Termitomyces albuminosus with black skin cultural method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105462869A (en) * | 2014-08-07 | 2016-04-06 | 马前 | Clostridium bifermentans and uses thereof, and nanometer metal sulfide preparation method |
| CN105695338A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus |
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| CN107484554A (en) * | 2017-09-29 | 2017-12-19 | 贵州棒棒食用菌产业有限公司 | A kind of artificial collybia albuminosa cultural method |
| CN109673379A (en) * | 2017-09-29 | 2019-04-26 | 贵州棒棒食用菌产业有限公司 | A kind of collybia albuminosa cultural method |
| CN107950303A (en) * | 2017-11-29 | 2018-04-24 | 四川山水美地农业投资有限公司 | A kind of cultural method of Termitomyces albuminosus with black skin parent species |
| CN110036987A (en) * | 2019-06-03 | 2019-07-23 | 昆明市林业和草原科学研究所 | Utilize the method for artificial culture ant nest fungus garden cultivation collybia albuminosa |
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