CN104303845A - Method for cultivating lucid ganoderma through thorns crumbs - Google Patents
Method for cultivating lucid ganoderma through thorns crumbs Download PDFInfo
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- CN104303845A CN104303845A CN201410611995.5A CN201410611995A CN104303845A CN 104303845 A CN104303845 A CN 104303845A CN 201410611995 A CN201410611995 A CN 201410611995A CN 104303845 A CN104303845 A CN 104303845A
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- twigs
- chaste tree
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- glossy ganoderma
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- 238000000034 method Methods 0.000 title claims abstract description 26
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- 239000001963 growth medium Substances 0.000 claims abstract description 19
- 235000012255 calcium oxide Nutrition 0.000 claims abstract description 18
- 239000000292 calcium oxide Substances 0.000 claims abstract description 18
- 229920000742 Cotton Polymers 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 230000006698 induction Effects 0.000 claims abstract description 7
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- 238000009472 formulation Methods 0.000 claims description 2
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- 108010029541 Laccase Proteins 0.000 description 5
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- 244000025254 Cannabis sativa Species 0.000 description 2
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- 241000222382 Agaricomycotina Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
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- 241000196324 Embryophyta Species 0.000 description 1
- 244000287680 Garcinia dulcis Species 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 240000005319 Sedum acre Species 0.000 description 1
- 235000014327 Sedum acre Nutrition 0.000 description 1
- 241001091465 Sempervivum Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 244000248021 Vitex negundo Species 0.000 description 1
- 235000010363 Vitex negundo Nutrition 0.000 description 1
- 244000029684 Vitex negundo var. negundo Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 235000009347 chasteberry Nutrition 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a method for cultivating lucid ganoderma through thorns crumbs, wherein the cultivation culture medium for lucid ganoderma comprises the components in parts by weight as follows: 60% of thorns crumbs, 20% of cotton seed hull, 19% of bran and 1% of quick lime; the preparation method is as follows: crushing the thorns strips into the crumbs, preparing the bran and quick lime; the ratio of the material and the water is 1: 1.4. The preparing technology taking the thorns crumbs as the main material for cultivating lucid ganoderma further comprises the operation as follows: the thorns crumbs is used as the substrate induction and added ahead of schedule in the liquid strain culture medium and grain strain culture medium for the lucid ganoderma. The method for cultivating lucid ganoderma through thorns crumbs takes full advantage of forestry waste thorns strips for forming the circular economy mode, avoiding resource waste, reducing the cultivating cost with high yield and short period. The method for cultivating lucid ganoderma through thorns crumbs has prominent economic benefit, ecological benefit and wide application prospect.
Description
Technical field
The invention belongs to the technical field of edible fungus cultivation, being specifically related to a kind of Ganodermataceae Dook culture material and the method for cultivating glossy ganoderma thereof that comprise twigs of the chaste tree bits.
Background technology
Glossy ganoderma (Ganoderma lucidum) belongs to fungus circle, Basidiomycota, Hymenomycetes, glossy ganoderma order, Ganodermataceae, Ganoderma in genealogical classification, is the fungi that a kind of medical value is very high.The fungus resource of China's Ganodermataceae is abundant especially, has now 4 genus 98 kinds.Glossy ganoderma is otherwise known as " polyporus lucidus ", " celestial grass ", " auspicious grass ", is also houseleek, whorlleaf stonecrop herb, has long medicinal history in China.Containing monose and polysaccharide in glossy ganoderma carbohydrate, the mainly polysaccharide wherein worked, polysaccharide accounts for greatly the 2.5%-5% of glossy ganoderma carbohydrate.In glossy ganoderma, amino acid content is very high, and kind is a lot, has very high nutritive value.In addition, containing a variety of vitamin in glossy ganoderma, comprising vitamin C, B
1, B
2, B
12and vitamin D, they are the indispensable nutriments of human body.Ganoderma lucidum fruitbody, conidial powder and its extract safety and do not have toxic and side effect, have antitumor; Regulate immunity of organisms; Calm, calm the nerves; Hypotensive; Antibechic, to eliminate the phlegm; Hypoglycemic, elimination free radical; Prevent and treat very many-sided pharmacological actions such as neural disease, current glossy ganoderma market prospects are boundless.
Cotton seed hull is because it is nutritious, air capacity of soils is good, it is the most frequently used planting material of edible mushroom high-yield culturing, but due to cotton seed hull rise in price in recent years, planting edible mushroom cost raises, economic benefit declines, so find the cultivation new raw material that can replace cotton seed hull, becomes a current large problem in the industry, meanwhile, the resource exploitation of other agriculture and forestry organic waste material is also conducive to.
The twigs of the chaste tree (Vitex negundo var ineica) popular name Jing Zi, the chaste tree tip, strip etc., classification belongs to Verenaceae, Vitex, a mutation of five-leaved chaste tree, machaka or dungarunga, distribute several all over the whole nation, especially more remarkable with the coastal area of Jiaodong Peninsula, all there is distribution on the ground such as ground, the Beishan Mountain, Ji, southeast Shanxi mountain region, Taihang Mountain, the western mountain and hill in Henan and loess plateau, Shan, Shanxi.Very extensive in the distribution of Hebei hilly region in Taihang Mountains, constitute the dominant shrub species seeds that early tailo is done in this district.We adopt and generally investigate the method that is combined with focal investigation and investigate, and the twigs of the chaste tree are machaka, like light, drought resisting impoverishment tolerant is thin, primary growth on hillside, roadside, the ground such as border earthen mound.Twigs of the chaste tree adaptability is extremely strong, requires not tight, all kinds of soil all has growth to soil.Twigs of the chaste tree seed germination power is strong, and Seedling propagation is easy, and people closes the deserted mountain containing a small amount of twigs of the chaste tree weeds etc., and 3-5 can plants burst out in profusion.Taihang Mountain, Hebei twigs of the chaste tree aboundresources, widely distributed, many places are in wild state according to investigations, there is no larger utilization, and main utilization ways is at present:
(1) chaste tree is compiled: the twigs of the chaste tree producing region masses, adopts bar and weaves various basket basket, to use by oneself, and little export trade.In addition, some small-size coal mine twigs of the chaste tree weave float, do frame use, but consumption are little for tunnel end face.
(2) firewood charcoal: the twigs of the chaste tree have resistance to stump, feature that Fuelwood product is high, manyly to use as combustion bavin, but utilization is extremely limited so local.
Compile with regard to traditional Land use systems chaste tree, total utilization of the twigs of the chaste tree also not enough stock number 10%, research shows have cellulose, lignin in the twigs of the chaste tree, these edible and medicinal fungis of half fiber grow necessary nutriment, also containing abundant organic matter and the nutritive element required for crop growth.But up to the present, the twigs of the chaste tree are regarded as agriculture and forestry organic waste material always and do not obtain fully and effectively developing, and seriously waste resource.Along with low-carbon environment-friendly and living resources recycle going deep into of idea, utilize twigs of the chaste tree culturing edible fungus, the twigs of the chaste tree not only can be made to turn waste into wealth, make its alternative traditional cultivation matrix cotton seed hulls, become the new medium of culturing edible fungus, simultaneously for more opportunity is created in increasing peasant income.
Summary of the invention
The object of this invention is to provide a kind of twigs of the chaste tree that utilize and cultivate the method for glossy ganoderma, solve the problem of the wild twigs of the chaste tree waste of current Taihang mountain areas, at environmentally safe and the living resources realizing agriculture and forestry organic waste material under the prerequisite of high yield recycle.
In order to realize object of the present invention, the invention provides following technical scheme.
The method of cultivating glossy ganoderma considered to be worth doing by this twigs of the chaste tree that utilize, and innovative point is that the culture medium for cultivating weight composition of glossy ganoderma comprises: twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quicklime 1%; The twigs of the chaste tree are ground into wood chip by preparation method, are equipped with wheat bran and quicklime, and material-water ratio is 1: 1.4.
The method of cultivating glossy ganoderma considered to be worth doing by the described twigs of the chaste tree that utilize, also as substrate for induction thing, add twigs of the chaste tree bits in advance in the liquid spawn culture medium of glossy ganoderma; It optimizes liquid spawn culture medium formulation weight ratio: potato 20%, twigs of the chaste tree powder 5%, wheat bran 3%, glucose 2%, KH
2pO
40.3%, MgSO
40.15%, VB
110mg/L, carboxymethyl cellulose 0.3%, water 1000mL, pH nature.
The method of cultivating glossy ganoderma considered to be worth doing by the described twigs of the chaste tree that utilize, also as substrate for induction thing, add twigs of the chaste tree bits in advance in the grain spawn medium of glossy ganoderma; Its grain spawn culture medium prescription weight ratio optimized is: wheat 69%, twigs of the chaste tree bits 30%, quicklime 1%.
The method of cultivating glossy ganoderma considered to be worth doing by the described twigs of the chaste tree that utilize, and comprises the steps:
A. the preparation of liquid spawn
A. the preparation of level liquid bacterial classification
Under aseptic condition, commercially available mother is planted in access level liquid medium, 28 DEG C, 160r/min constant-temperature shaking culture 5d, i.e. obtained level liquid bacterial classification;
Described level liquid bacterium culture medium comprises: by weight percentage, potato 20%, wheat bran 3%, glucose 2%, KH
2pO
40.3%, MgSO
40.15%, CMC0.3%, VB
110mg/L;
B. the preparation of second-class liquid isolate
Under aseptic condition, by level liquid bacterial classification with in the inoculum concentration access secondary liquid medium of 10%, 28 DEG C, 160r/min constant-temperature shaking culture 7d, i.e. obtained second-class liquid isolate;
Described second-class liquid isolate culture medium prescription is: by weight percentage, potato 20%, twigs of the chaste tree bits powder 5%, wheat bran 3%, glucose 2%, KH
2pO
40.3%, MgSO
40.15%, CMC0.3%, VB
110mg/L;
B. the preparation of grain spawn
The second-class liquid isolate access grain spawn medium that steps A is obtained, inoculum concentration is 10%, 22-25 DEG C and is cultured to full, obtains grain spawn;
Described grain spawn medium comprises: wheat 69%, twigs of the chaste tree bits 30%, quicklime 1%.
C. the preparation of twigs of the chaste tree bits composts or fertilisers of cultivating
Twigs of the chaste tree branch is pulverized as wood chip shape all in advance;
Twigs of the chaste tree bits composts or fertilisers of cultivating composition comprises: twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quicklime 1%;
Accurately take each constituent of twigs of the chaste tree bits medium, add water after mixing, pile vexed 30 min, load in container, sterilizing, for subsequent use after cooling;
D. inoculate, cultivate, gather
Under aseptic condition, the twigs of the chaste tree bits composts or fertilisers of cultivating that the grain spawn that prepared by step B access step C prepares, carries out cultivating, management of producing mushroom, to gather.
The method of cultivating glossy ganoderma considered to be worth doing by the described twigs of the chaste tree that utilize, and in step D, the inoculum concentration of grain spawn is 5-10%.
Cultivation glossy ganoderma method considered to be worth doing by the described twigs of the chaste tree that utilize, and in step C, the container of splendid attire twigs of the chaste tree bits composts or fertilisers of cultivating selects Polythene Bag, Polypropylene Bag or vial.
The method of cultivating glossy ganoderma considered to be worth doing by the described twigs of the chaste tree that utilize, and in step C, the sterilising conditions of twigs of the chaste tree bits composts or fertilisers of cultivating is 121 DEG C of autoclaving 150min or 100 DEG C normal-pressure sterilization 24h.
The present invention is used for the medium of cultivating ganoderma, and with twigs of the chaste tree bits for primary raw material, effect has three: 1, for edible mushroom, grows required carbon source for it provides.2, the twigs of the chaste tree are not yet developed at present, aboundresources, and for edible fungus culturing provides a kind of cheap culturing raw material, production cost is low, high financial profit.3, from the angle of ecology, achieve the recycling of agriculture and forestry organic waste material, there is considerable economic benefit, have a extensive future.
Embodiment
Below in conjunction with specific embodiment, content of the present invention is further described in detail.
Embodiment 1, utilize the twigs of the chaste tree to consider to be worth doing to cultivate the method for glossy ganoderma, lucidum strain is market purchasing
A. the preparation of liquid spawn
Level liquid Spawn incubation based formulas: potato 20%, wheat bran 3%, glucose 2%, KH
2pO
40.3%, MgSO
40.15%, VB
110mg/L, CMC0.3%, pH nature;
Second-class liquid isolate culture medium prescription: twigs of the chaste tree bits powder 5%, potato 20%, wheat bran 3%, glucose 2%, KH
2pO
40.3%, MgSO
40.15%, VB
110mg/L, CMC0.3%, pH nature; Wherein twigs of the chaste tree bits were pulverized 80 mesh sieves and were obtained powdery, and boil 30min altogether with potato, wheat bran, filter and remove residue obtains leaching liquor.
Use 500mL conical flask time prepared by level liquid bacterial classification, liquid amount is 200mL, after 121 DEG C of autoclaving 30min, accesses commercially available PDA slant strains under aseptic condition, and every bottle of inoculum concentration is 4 pieces of 0.5cm × 0.5cm bacterial classification blocks.After inoculation 28 DEG C, under the condition of 160r/min, constant-temperature shaking culture 5d.
Second-class liquid isolate liquid amount specification, sterilising conditions and condition of culture are the same, and inoculum concentration is 10%.
At second-class liquid isolate preparatory phase, measure and optimize laccase activity and carboxymethylcelluloenzyme enzyme enzyme (CMC enzyme is lived) alive in liquid fungus seed liquid, not add the medium of twigs of the chaste tree crumbs powder for contrast, to find whether twigs of the chaste tree powder has the advantage of substrate for induction effect in liquid spawn.Enzyme activity determination result is as shown in table 1.
Laccase activity defines: under certain condition, the enzyme amount of reactant liquor OD value increase by 0.001 that makes per minute is defined as a laccase activity unit of force (U).
Carboxymethylcelluloenzyme enzyme enzyme activity (CMC enzyme is lived) definition: under certain condition, the enzyme amount that enzymolysis substrate per minute produces 1 μ g glucose is defined as a carboxymethylcelluloenzyme enzyme enzyme activity unit (U).
The enzyme live data of table 1 ganoderma strain capable
As shown in Table 1, the laccase activity of secondary liquid medium of the present invention, CMC enzyme are lived all than the height of control group (not adding twigs of the chaste tree bits), add twigs of the chaste tree bits in visible liquid medium within and certain substrate for induction effect is played to the laccase of Ganoderma lucidum mycelium and the secretion of cellulase, mycelia is allowed to adapt to substrate (twigs of the chaste tree bits) in advance, thus accelerate the long speed of mycelia in planting material, shorten cultivation period.
B. the preparation of grain spawn
Grain spawn culture medium prescription: wheat 69%, twigs of the chaste tree bits 30%, quicklime 1%.
Accurately take each constituent of medium, material-water ratio 1 ︰ 1.4 carries out spice, and load 17cm × 32cm × 0.05cm polyethylene plastic bag, every packed siccative 300g, prepares 120 bags.The control group liquid spawn optimized the second-class liquid isolate that obtains and do not add twigs of the chaste tree bits is accessed grain spawn medium respectively, inoculates 60 bags respectively, measure the long speed of bacterium, and time, purseful time completely takeed in record.
In order to investigate the advantage of grain spawn medium of the present invention, invention has been comparative trial, control group medium is wheat medium: wheat 99%, quicklime 1%; Comparative test result is as shown in table 2.
The purseful time of table 2 glossy ganoderma grain spawn
As shown in Table 2, mycelia is faster than the long speed of pure wheat medium in " wheat+twigs of the chaste tree bits " grain culture medium, this is because add twigs of the chaste tree bits in liquid medium within, enables the faster Adaptable growth substrate of mycelia, thus accelerate to send out bacterium speed, shorten the production cycle and enhance productivity.To sum up, wheat+twigs of the chaste tree bits can replace cotton seed hull to become the major ingredient of glossy ganoderma plantation Central Plains kind making, and this discarded object of the twigs of the chaste tree have also been obtained recycling, is of great importance to the Efficient Cultivation of glossy ganoderma.
C. the preparation of twigs of the chaste tree bits composts or fertilisers of cultivating
In mass ratio, take twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quicklime 1%, quicklime 1%, material-water ratio is that 1 ︰ 1.4 carries out spice, after mixing, loads in container and carry out sterilizing and inoculation work after piling vexed 30 min.
Loaded in Polythene Bag, Polypropylene Bag or vial by above-mentioned composts or fertilisers of cultivating, bore is not limit, conventional tying, and autoclaving is generally with 121 DEG C of sterilizing 150min; If normal-pressure sterilization is generally with 100 DEG C of sterilizings, keep 24h; Inoculation after cooling.
The present invention investigates the consumption of twigs of the chaste tree bits in research process, specific as follows:
Accurately take each constituent by shown in table 3, mixed, add water, material-water ratio is 1 ︰ 1.4, and the composts or fertilisers of cultivating configured is loaded test tube, and each formula repeats 5 times, 121 DEG C of autoclaving 150 min.Grain spawn described in step B is accessed respectively in the test tube containing composts or fertilisers of cultivating, carry out test tube and to nourish and grow test.
Table 3 culture medium prescription (siccative, %)
| Formula | The twigs of the chaste tree are considered to be worth doing | Cotton seed hull | Wheat bran | Quicklime |
| P0 | 0 | 20 | 19 | 1 |
| P1 | 30 | 20 | 19 | 1 |
| P2 | 40 | 20 | 19 | 1 |
| P3 | 50 | 20 | 19 | 1 |
| P4 | 60 | 20 | 19 | 1 |
| P5 | 70 | 20 | 19 | 1 |
| P6 | 0 | 80 | 19 | 1 |
Observe long speed and the growing way situation of mycelia in a test tube every 2 days, measure the long speed of mycelia with slide measure (accuracy 0.02mm).Growth rata and potential of hypha result is as shown in table 4.
The mycelial growth situation of table 4 glossy ganoderma
| Formula | Mycelium length (mm/d) | Mycelium growth vigor |
| P6 | 7.65±0.01 | +++ |
| P4 | 7.21±0.02 | +++ |
| P5 | 7.15±0.03 | +++ |
| P3 | 6.92±0.05 | +++ |
| P1 | 6.47±0.04 | ++ |
| P2 | 6.15±0.01 | ++ |
| P0 | 6.03±0.02 | + |
(note: list long speed and be mean value ± standard error; +++ represent that growth is fine and close, ++ represent that growth is finer and close ,+represent that growth is general.)
As can be seen from Table 4, when twigs of the chaste tree bits addition slightly increases, its growing way is better, and mycelia is pure white, finer and close, has comparativity with pure cotton seed hull medium, and mycelia grows the fastest on formula P6.
Each composition is taken by the formula in table 3, mix, material-water ratio 1 ︰ 1.4 carries out spice, load 17cm × 32cm × 0.05cm polyethylene plastic bag, every packed siccative about 300 g, the grain spawn one that inoculum concentration is " liquid spawn → twigs of the chaste tree bits+cotton seed hull " production of hybrid seeds inoculates spoon, observes and records the growing state of each formula and calculate biological transformation ratio.Result of the test is as shown in table 5.
The biological transformation ratio of table 5 glossy ganoderma
| Medium is numbered | Average yield (g) | Average organism efficiency (%) |
| P6 | 223.62 | 74.54 |
| P4 | 219.30 | 73.10 |
| P3 | 194.85 | 64.95 |
| P5 | 186.9 | 62.30 |
| P1 | 179.64 | 59.88 |
| P2 | 166.29 | 55.43 |
| P0 | 155.16 | 51.72 |
As can be seen from Table 5, when with twigs of the chaste tree bits for primary carbon source reaches 60%, the biological transformation ratio of ganoderma strain capable reaches 73.10%, although do not reach the biological transformation ratio 74.54% of the pure cotton seed hull of planting material major ingredient, has comparativity.Illustrate that twigs of the chaste tree bits can partly replace cotton seed hull cultivating ganoderma.
The present invention finally determines that Optimum formulae is twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quicklime 1%, and material-water ratio is that 1 ︰ 1.4 carries out spice.
D. inoculate, cultivate, gather
By cooled for sterilizing fruiting bag, according to the inoculum concentration access step B gained grain spawn of 5-10% under sterile working.Carry out lucifuge cultivation under the bacterium bag to connect kind is placed in 25-28 DEG C of room temperature, bacterial classification sprouted as early as possible, field planting.After mycelia purseful, unlimited sack carries out reproductive growth management phase.
When going out sesame, canopy temperature remains on 26-30 DEG C, and relative air humidity wants 90%-95%, and provides the oxygen of scattered light and abundance.Keep ground to have shoaling layer, spray water once to soil surrounding and space every day.When every morning 8 before and afternoon 4 time after to raise isolated film come ventilating opening take a breath.Former base has expanded 3-5 days, slowly forms cap, increase water spray insulation, if the too high temperature control of will spraying water of temperature.If improper ventilation easily goes out lopsided sesame, to slice off if there is abnormal bud at once.When cap rises bronzing by white-pale yellow-Huang, the edge white of cap is basic to disappear, and edge starts to redden, cap keratin, when vaporific spore russet has been launched out at the cap back side, illustrates that ganoderma lucidum fruitbody is ripe, just can gather in time.
In sum, using the living resources of the twigs of the chaste tree as reusable edible, for the industrialization development of high temperature modification edible mushroom glossy ganoderma, there is important innovative significance.
Claims (9)
1. utilize the twigs of the chaste tree to consider a method of cultivating glossy ganoderma to be worth doing, its feature comprises: the culture medium for cultivating weight composition of glossy ganoderma comprises: twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quicklime 1%; The twigs of the chaste tree are ground into wood chip by preparation method, are equipped with wheat bran and quicklime, and material-water ratio is 1: 1.4.
2. according to claim 1ly utilize the twigs of the chaste tree to consider to be worth doing to cultivate the method for glossy ganoderma, its feature comprises: also as substrate for induction thing, add twigs of the chaste tree bits in advance in the liquid spawn culture medium of glossy ganoderma; It optimizes liquid spawn culture medium formulation weight ratio: potato 20%, twigs of the chaste tree powder 5%, wheat bran 3%, glucose 2%, KH
2pO
40.3%, MgSO
40.15%, VB
110mg/L, carboxymethyl cellulose 0.3%, water 1000mL, pH nature.
3. according to claim 1ly utilize the twigs of the chaste tree to consider to be worth doing to cultivate the method for glossy ganoderma, its feature comprises: also as substrate for induction thing, add twigs of the chaste tree bits in advance in the grain spawn medium of glossy ganoderma; Its grain spawn culture medium prescription weight ratio optimized is: wheat 69%, twigs of the chaste tree bits 30%, quicklime 1%.
4. the method for cultivating glossy ganoderma considered to be worth doing by the twigs of the chaste tree that utilize according to claim 1, and its feature comprises the steps:
A. the preparation of liquid spawn
A. the preparation of level liquid bacterial classification
Under aseptic condition, commercially available mother is planted in access level liquid medium, 28 DEG C, 160r/min constant-temperature shaking culture 5d, i.e. obtained level liquid bacterial classification;
Described level liquid bacterium culture medium comprises: by weight percentage, potato 20%, wheat bran 3%, glucose 2%, KH
2pO
40.3%, MgSO
40.15%, CMC0.3%, VB
110mg/L;
B. the preparation of second-class liquid isolate
Under aseptic condition, by level liquid bacterial classification with in the inoculum concentration access secondary liquid medium of 10%, 28 DEG C, 160r/min constant-temperature shaking culture 7d, i.e. obtained second-class liquid isolate;
Described second-class liquid isolate culture medium prescription is: by weight percentage, potato 20%, twigs of the chaste tree bits powder 5%, wheat bran 3%, glucose 2%, KH
2pO
40.3%, MgSO
40.15%, CMC0.3%, VB
110mg/L;
B. the preparation of grain spawn
The second-class liquid isolate access grain spawn medium that steps A is obtained, inoculum concentration is 10%, 22-25 DEG C and is cultured to full, obtains grain spawn;
Described grain spawn medium comprises: wheat 69%, twigs of the chaste tree bits 30%, quicklime 1%.
C. the preparation of twigs of the chaste tree bits composts or fertilisers of cultivating
Twigs of the chaste tree branch is pulverized as wood chip shape all in advance;
Twigs of the chaste tree bits composts or fertilisers of cultivating composition comprises: twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quicklime 1%;
Accurately take each constituent of twigs of the chaste tree bits medium, add water after mixing, pile vexed 30min, load in container, sterilizing, for subsequent use after cooling;
D. inoculate, cultivate, gather
Under aseptic condition, the twigs of the chaste tree bits composts or fertilisers of cultivating that the grain spawn that prepared by step B access step C prepares, carries out cultivating, management of producing mushroom, to gather.
5. utilize the twigs of the chaste tree to consider the method for cultivating glossy ganoderma to be worth doing according to claim 4, it is characterized in that, in step D, the inoculum concentration of grain spawn is 5-10%.
6. utilize the twigs of the chaste tree to consider to be worth doing according to claim 1 and cultivate glossy ganoderma method, it is characterized in that, in step C, the container of splendid attire twigs of the chaste tree bits composts or fertilisers of cultivating selects Polythene Bag, Polypropylene Bag or vial.
7. utilize the twigs of the chaste tree to consider the method for cultivating glossy ganoderma to be worth doing according to claim 1, it is characterized in that, in step C, the sterilising conditions of twigs of the chaste tree bits composts or fertilisers of cultivating is 121 DEG C of autoclaving 150min or 100 DEG C normal-pressure sterilization 24h.
8. utilize the twigs of the chaste tree to consider the method for cultivating glossy ganoderma to be worth doing according to claim 2, it is characterized in that, in liquid nutrient medium, twigs of the chaste tree bits used, pulverize and cross 80 mesh sieves in advance.
9. utilize the twigs of the chaste tree to consider the method for cultivating glossy ganoderma to be worth doing according to claim 3, it is characterized in that, in grain spawn medium, twigs of the chaste tree bits used, pulverize in advance as wood chip shape.
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| CN109845582A (en) * | 2019-01-07 | 2019-06-07 | 贵州正黔门大健康产业有限公司 | A kind of preparation method of Coriaria sinica mushroom liquid bacterial |
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| CN109845582A (en) * | 2019-01-07 | 2019-06-07 | 贵州正黔门大健康产业有限公司 | A kind of preparation method of Coriaria sinica mushroom liquid bacterial |
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