CN105165387A - Method for culturing pleurotus geesteranus by using wild-jujube branch sawdust - Google Patents

Method for culturing pleurotus geesteranus by using wild-jujube branch sawdust Download PDF

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CN105165387A
CN105165387A CN201510407772.1A CN201510407772A CN105165387A CN 105165387 A CN105165387 A CN 105165387A CN 201510407772 A CN201510407772 A CN 201510407772A CN 105165387 A CN105165387 A CN 105165387A
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ziziphi spinosae
ramulus ziziphi
bits
medium
elegant precious
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王谦
申小燕
刘敏
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Hebei University
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Hebei University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

Abstract

The invention provides a method for culturing pleurotus geesteranus by using wild-jujube branch sawdust as a main material. The method comprises the following steps: (A) preparing and culturing a liquid strain; (B) preparing and culturing a cereal grain liquid strain; (C) preparing a culture medium for cultivation; and (D) inoculating the cereal grain liquid strain prepared in the step (B) to the culture medium prepared in the step (C) under sterile conditions, and carrying out culturing, mushroom producing management and harvesting. The innovation point of the method is as follows: culture media in the step (A) or/and the step (B) or/and the step (C) contain the wild-jujube branch sawdust. According to the method, forestry waste, i.e., wild jujube branches is fully utilized, a circular economy mode is formed, the wasting of natural resources and the pollution to environment are avoided, and the cultivation cost is reduced. The culture technique for culturing the pleurotus geesteranus by using the wild-jujube branch sawdust has remarkable economic and ecological benefits and has a broad application prospect.

Description

A kind of Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing
Technical field
The invention belongs to the technical field of edible fungus cultivation, being specifically related to a kind ofly comprise the precious mushroom composts or fertilisers of cultivating of show of Ramulus Ziziphi Spinosae bits and the method for cultivating elegant precious mushroom thereof.
Elegant precious mushroom (Pleurotusgeesteranus) belongs to Eumycota, Basidiomycetes, Agaricales, Pleurotaceae Pleurotus on the precious mushroom of taxonomy show.Title derives from Taiwan, and it is different from common phoenix-tail mushroom is that handle has 5 ~ 6 centimetres, lid diameter <3 centimetre because it is less.Elegant precious mushroom is the denseer title of a business taste of phoenix-tail mushroom in fact, is the prematurity fruit body of phoenix-tail mushroom.
In recent years, the exploitation of elegant precious mushroom had become popular topic with research on Japan, Korea S, China Taiwan and other places.The intelligent without seeming so precious mushroom plantation of current state was developed already, and planned, the elegant precious mushroom of intensivism ground development produces, and developing elegant precious mushroom series of products, is the work that of developing in fungi cause has realistic meaning very much.
Wild jujube is a kind of shrub of Rhamnaceae zizyphus.For a kind of wild resource that northern China is more common, be distributed in the Hills And Low Mountains of height above sea level 200-800 rice more, only for Hebei, 48.1% of Taihang Mountain, the Hebei gross area can be accounted for.The purposes of usual Ramulus Ziziphi Spinosae is: make bavin and burn, be used as hedge revetment, be used as the protecting wall in orchard, as the use of checking winds and fixing drifting sand.At present, not yet carry out the exploitation of wild jujube branch resource, its abundant wild resource can not fully be utilized, and causes great waste.With Ramulus Ziziphi Spinosae bits for culturing raw material, very large economy and ecological benefits will inevitably be produced, improve local economy structural adjustment, realizing will playing huge effect in recycling economy.
Summary of the invention
The object of this invention is to provide a kind of Ramulus Ziziphi Spinosae that utilizes and consider the method for cultivating elegant precious mushroom to be worth doing, the living resources realizing this elite stand discarded object of Ramulus Ziziphi Spinosae recycle.
To achieve these goals, the invention provides a kind of Ramulus Ziziphi Spinosae that utilizes and consider the method for cultivating Uricularia polytricha to be worth doing, comprise the steps:
A. the preparation of liquid spawn and cultivation;
B. the preparation of grain spawn and cultivation;
C. the preparation of cultivation medium;
D., under aseptic condition, the medium that the grain spawn that prepared by step B access step C prepares, carries out cultivating, management of producing mushroom, to gather;
Innovative point be in steps A or/and step B or/and step C medium in containing Ramulus Ziziphi Spinosae bits.
The described Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing, in steps A elegant precious mushroom liquid spawn culture medium in add Ramulus Ziziphi Spinosae bits in advance as substrate for induction thing; It optimizes liquid spawn culture medium formulation weight percentage: potato 20%, Ramulus Ziziphi Spinosae powder 5%, wheat bran 3%, glucose 2%, KH 2pO 40.3%, MgSO 40.15%, VB110mg/L, carboxymethyl cellulose 0.3%, water 1000mL, pH nature.
The described Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing, and in described steps A, the preparation of liquid spawn is characterised in that:
A. the preparation of level liquid bacterial classification
Under aseptic condition, commercially available mother is planted in access level liquid medium, 26-28 DEG C, 160r/min constant-temperature shaking culture 5d, i.e. obtained level liquid bacterial classification;
Described level liquid bacterium culture medium comprises: by weight percentage, potato 20%, wheat bran 3%, glucose 2%, KH 2pO 40.3%, MgSO 40.15%, CMC0.3%, VB 110mg/L;
B. the preparation of second-class liquid isolate
Described second-class liquid isolate culture medium prescription percentage by weight is: potato 20%, Ramulus Ziziphi Spinosae powder 5%, wheat bran 3%, glucose 2%, KH 2pO 40.3%, MgSO 40.15%, CMC0.3%, VB 110mg/L.Potato, Ramulus Ziziphi Spinosae bits powder and the wheat bran 1000mL that adds water boil by preparation method altogether, keep micro-and boil 30 minutes, filter, get filtrate, add other component while hot, stir after melting, be settled to water 1000mL, packing, sterilizing after boiling, to be seeded;
Under aseptic condition, by level liquid bacterial classification with in the inoculum concentration access secondary liquid medium of 10%, 26-28 DEG C, 160r/min constant-temperature shaking culture 7d, i.e. obtained second-class liquid isolate.
The described Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing, in step B elegant precious mushroom grain spawn medium in also add Ramulus Ziziphi Spinosae bits in advance as substrate for induction thing; Its grain spawn culture medium prescription optimized: wheat 84%, Ramulus Ziziphi Spinosae bits 15%, quicklime 1%.
The described Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing, and in described step B, the preparation of grain spawn is characterised in that:
Second-class liquid isolate access grain spawn medium steps A obtained, inoculum concentration is 10%, 26-28 DEG C and is cultured to full, obtains grain spawn.
The described Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing, and in step C, Ramulus Ziziphi Spinosae bits are as the major ingredient of the elegant precious mushroom of cultivation, and culture medium for cultivating weight composition comprises: Ramulus Ziziphi Spinosae bits 60%, cotton seed hull 20%, wheat bran 19%, quicklime 1%.
The described Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing, and in described step C, the preparation of Ramulus Ziziphi Spinosae bits medium is characterised in that: Ramulus Ziziphi Spinosae, reed powder are broken into wood chip shape, are equipped with cotton seed hull, wheat bran and quicklime, material-water ratio is 1: 1.4, piles vexed 30min, loads in container, sterilizing, for subsequent use after cooling.
The described Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing, and in step D, the inoculum concentration of grain spawn is 5-10%.
The described method utilizing Ramulus Ziziphi Spinosae to consider the elegant precious mushroom of medium cultivation to be worth doing, in liquid nutrient medium, Ramulus Ziziphi Spinosae bits used, pulverize and cross 80 mesh sieves in advance.
The described Ramulus Ziziphi Spinosae that utilizes considers the method for cultivating elegant precious mushroom to be worth doing, and in step C, the sterilising conditions of Ramulus Ziziphi Spinosae bits medium is 121 DEG C of autoclaving 150min or 100 DEG C normal-pressure sterilization 24h.
Technological progress effect of the present invention shows the primary raw material using Ramulus Ziziphi Spinosae bits as cultivating Uricularia polytricha, and advantage mainly contains 3 points:
1, for edible mushroom, for it provides growth required carbon source.
2, Ramulus Ziziphi Spinosae is not yet developed at present, aboundresources, and for edible fungus culturing provides a kind of cheap culturing raw material, production cost is low, high financial profit.
3, from the angle of ecology, achieve the recycling of agriculture and forestry organic waste material, there is considerable economic benefit, have a extensive future.
Embodiment
Below in conjunction with specific embodiment, content of the present invention is further described in detail.
Embodiment: utilize Ramulus Ziziphi Spinosae to consider to be worth doing and cultivate elegant precious mushroom, oyster mushroom kind is market purchasing.
If employing common process, then it is the female kind-cotton seed hull original seed of PDA-Ramulus Ziziphi Spinosae bits cultivated species-Ramulus Ziziphi Spinosae bits fruiting bag; But adopt the female kind-liquid spawn-grain spawn of PDA-Ramulus Ziziphi Spinosae bits fruiting bag technique, then can shorten the production cycle nearly 1/3rd.Introduce liquid spawn technique below:
A. the preparation of liquid spawn
Level liquid Spawn incubation based formulas: potato 20%, wheat bran 3%, glucose 2%, KH 2pO 40.3%, MgSO 40.15%, VB 110mg/L, CMC0.3%, pH nature; Preparation method is potato, wheat bran boils 30min altogether, and filter and remove residue obtains leaching liquor and adds other compositions.Use 500mL conical flask time prepared by level liquid bacterial classification, liquid amount is 200mL, after 121 DEG C of autoclaving 30min, accesses commercially available PDA slant strains under aseptic condition, and every bottle of inoculum concentration is 4 pieces of 0.5cm × 0.5cm bacterial classification blocks.After inoculation 28 DEG C, under the condition of 160r/min, constant-temperature shaking culture 7d.
Second-class liquid isolate culture medium prescription: Ramulus Ziziphi Spinosae bits powder 5%, potato 20%, wheat bran 3%, glucose 2%, KH 2pO 40.3%, MgSO 40.15%, VB 110mg/L, CMC0.3%, pH nature; Wherein Ramulus Ziziphi Spinosae bits were pulverized 80 mesh sieves and were obtained powdery, and boil 30min altogether with potato, wheat bran, filter and remove residue obtains leaching liquor.
Preparation method is that Ramulus Ziziphi Spinosae bits were pulverized 80 mesh sieves and obtained powdery, and boil 30min altogether with potato, wheat bran, filter and remove residue obtains leaching liquor.Second-class liquid isolate liquid amount specification, sterilising conditions and condition of culture are the same, and inoculum concentration is 10%.
At second-class liquid isolate preparatory phase, measure and optimize laccase activity and carboxymethylcelluloenzyme enzyme enzyme (CMC enzyme is lived) alive in liquid fungus seed liquid, not add the medium of Ramulus Ziziphi Spinosae crumbs powder for contrast, to find whether Ramulus Ziziphi Spinosae powder has the advantage of substrate for induction effect in liquid spawn.Enzyme activity determination result is as shown in table 1.
Laccase activity defines: under certain condition, the enzyme amount of reactant liquor OD value increase by 0.001 that makes per minute is defined as a laccase activity unit of force (U).
Carboxymethylcelluloenzyme enzyme enzyme activity (CMC enzyme is lived) definition: under certain condition, the enzyme amount that enzymolysis substrate per minute produces 1 μ g glucose is defined as a carboxymethylcelluloenzyme enzyme enzyme activity unit (U).
The enzyme live data of the elegant precious mushroom of table 1
Liquid nutrient medium Laccase activity (U/mL) CMC enzyme lives (U/mL)
Control group (not adding Ramulus Ziziphi Spinosae bits) 47.69 66.19
Liquid nutrient medium of the present invention 52.52 72.78
As shown in Table 1, the laccase activity of secondary liquid medium of the present invention, CMC enzyme are lived all than the height of control group (not adding Ramulus Ziziphi Spinosae bits), add Ramulus Ziziphi Spinosae bits in visible liquid medium within and certain substrate for induction effect is played to the laccase of oyster mushroom pieces and the secretion of cellulase, mycelia is allowed to adapt to substrate (Ramulus Ziziphi Spinosae bits) in advance, thus accelerate the long speed of mycelia in planting material, shorten cultivation period.
B. the preparation of grain spawn
Grain spawn culture medium prescription: wheat 84%, Ramulus Ziziphi Spinosae bits 15%, quicklime 1%.
Accurately take each constituent of medium, material-water ratio 1 ︰ 1.4 carries out spice, and load 17cm × 32cm × 0.05cm polyethylene plastic bag, every packed siccative 300g, prepares 120 bags.The control group liquid spawn optimized the second-class liquid isolate that obtains and do not add Ramulus Ziziphi Spinosae bits is accessed grain spawn medium respectively, inoculates 60 bags respectively, measure the long speed of bacterium, and record the purseful time.
In order to investigate the advantage of grain spawn medium of the present invention, invention has been comparative trial, control group medium is wheat medium: wheat 99%, quicklime 1%; Comparative test result is as shown in table 2.
The purseful time of the elegant precious mushroom grain spawn of table 2
Liquid spawn Grain culture medium Average purseful time (d)
Second-class liquid isolate of the present invention (adding Ramulus Ziziphi Spinosae crumbs powder) Wheat+Ramulus Ziziphi Spinosae bits 23
Pure wheat 28
Control group second-class liquid isolate (not adding Ramulus Ziziphi Spinosae bits) Wheat+Ramulus Ziziphi Spinosae bits 25
Pure wheat 28
As shown in Table 2, mycelia is faster than the long speed of pure wheat medium in " wheat+Ramulus Ziziphi Spinosae bits " grain culture medium, this is because the interpolation of gap between grain and Ramulus Ziziphi Spinosae bits is more conducive to the growth of mycelia, Ramulus Ziziphi Spinosae bits are added in liquid medium within, enable the faster Adaptable growth substrate of mycelia, thus accelerate to send out bacterium speed, shorten the production cycle and enhance productivity.
C. the preparation of elegant precious mushroom composts or fertilisers of cultivating
In mass ratio, take Ramulus Ziziphi Spinosae bits 60%, cotton seed hull 29%, wheat bran 10%, quicklime 1%, material-water ratio is that 1 ︰ 1.4 carries out spice, after mixing, loads in container and carry out sterilizing and inoculation work after piling vexed 30min.
Loaded in Polythene Bag, Polypropylene Bag or vial by above-mentioned composts or fertilisers of cultivating, bore is not limit, conventional tying, and autoclaving is generally with 121 DEG C of sterilizing 150min; Normal-pressure sterilization, generally with 100 DEG C of sterilizings, keeps 24h; Inoculation after cooling.
The present invention investigates the consumption of Ramulus Ziziphi Spinosae bits in research process, specific as follows:
Accurately take each constituent by shown in table 3, mixed, add water, material-water ratio is 1 ︰ 1.4, and the composts or fertilisers of cultivating configured is loaded test tube, and each formula repeats 5 times, 121 DEG C of autoclaving 150min.Grain spawn described in step B is accessed respectively in the test tube containing composts or fertilisers of cultivating, carry out test tube and to nourish and grow test.
Table 3 culture medium prescription (siccative, %)
Formula number Ramulus Ziziphi Spinosae is considered to be worth doing Cotton seed hull Wheat bran Lime
P0 0 89 10 1
P1 30 59 10 1
P2 40 49 10 1
P3 50 39 10 1
P4 60 29 10 1
P5 70 19 10 1
P6 80 9 10 1
Observe long speed and the growing way situation of mycelia in a test tube every 2 days, measure the long speed of mycelia with slide measure (accuracy 0.02mm).Growth rata and potential of hypha result is as shown in table 4.
The mycelial growth situation of the elegant precious mushroom of table 4
Formula Mycelium length (mm/d) Mycelium growth vigor
P0 5.66±0.01 +++
P1 5.50±0.02 +++
P2 5.45±0.04 +++
P5 5.36±0.03 +++
P3 5.23±0.01 ++
P4 5.18±0.02 ++
P6 5.02±0.03 +
Note: list long speed and be mean value ± standard error; +++ represent that growth is fine and close, ++ represent that growth is finer and close ,+represent that growth is general.
As can be seen from Table 4, as Ramulus Ziziphi Spinosae bits addition P4, its growing way is better, and mycelia is pure white, finer and close, has comparativity with pure cotton seed hull medium.
Each composition is taken by the formula in table 3P4, mix, material-water ratio 1 ︰ 1.4 carries out spice, load 17cm × 32cm × 0.05cm polyethylene plastic bag, every packed siccative is about 300g, the grain spawn one that inoculum concentration is " liquid spawn → Ramulus Ziziphi Spinosae bits+cotton seed hull " production of hybrid seeds inoculates spoon, observes and records the growing state of each formula and calculate biological transformation ratio.Result of the test is as shown in table 5.
The biological transformation ratio of the elegant precious mushroom of table 5
Medium is numbered Average yield (g) Average organism efficiency (%)
P0 216.30 72.10
P1 210.69 70.23
P4 207.30 69.10
P3 205.62 68.54
P2 194.07 64.69
P5 186.30 62.10
P6 183.66 61.22
As can be seen from Table 5, when with Ramulus Ziziphi Spinosae bits for primary carbon source reaches 60%, the biological transformation ratio of oyster mushroom strain reaches 69.10%, although do not reach the biological transformation ratio 72.10% of the pure cotton seed hull of planting material major ingredient, but Ramulus Ziziphi Spinosae bits can become the major ingredient in elegant precious mushroom plantation, Ramulus Ziziphi Spinosae is considered this forestry waste to be worth doing and be have also been obtained recycling, significant to ecological agriculture construction.
The present invention finally determines that Optimum formulae is Ramulus Ziziphi Spinosae bits 60%, cotton seed hull 29%, wheat bran 10%, quicklime 1%, and material-water ratio is that 1 ︰ 1.4 carries out spice.
D. inoculate, cultivate, gather
By cooled for sterilizing fruiting bag, according to the inoculum concentration access step B gained grain spawn of 5-10% under sterile working.Carry out lucifuge cultivation under the bacterium bag to connect kind can being placed in about 24 DEG C room temperatures, bacterial classification sprouted as early as possible, field planting.After mycelia purseful, unlimited sack carries out reproductive growth management phase.
Former base differential period should widen the temperature difference, makes to form the temperature difference needing 8 ~ 10 DEG C round the clock, gives appropriate scattered beam simultaneously, keeps relative air humidity 85% ~ 90%, stimulates mycelia to form comparatively neat former base.
After the fruit body former phase is formed, cultivation place will reduce epidemic disaster difference as far as possible, and temperature will control at 18 ~ 22 DEG C as far as possible, and relative air humidity is stabilized in 85% ~ 90%, and every day sprays water 3 ~ 5 times to aerial or ground, must guard against and sprays water on fruit body primordium.After each water spray, should forced ventilation be added, otherwise hot and humidly very easily cause courses of infection, find that charge level has ponding, fall clearly in time.After most of former base occurs, suitably can reduce the temperature difference, improve temperature of shed, be conducive to sporophore growth.When temperature of shed is more than 25 DEG C or lower than 8 DEG C, cooling should be managed or heat; Relative air humidity can fluctuate between 80% ~ 95%, larger in this scope humidity, and fruit body looks more plump, stocky.
Gather: cap grows to 2 ~ 3 centimetres, involute, the spore in cap edge not yet launches to gather and be advisable.Push down composts or fertilisers of cultivating on the other hand when gathering, catching mushroom body to reverse gently on the other hand can pull up.Elegant precious mushroom mostly is and grows thickly, must whole clump is disposable has gathered when gathering, and cleaning mushroom pin can carry out packaging and go on the market.
In sum, Ramulus Ziziphi Spinosae of the present invention is considered to be worth doing as major ingredient can be used for cultivating elegant precious mushroom, therefore, using the living resources of Ramulus Ziziphi Spinosae as reusable edible, for industrialization development, there is important innovative significance and realistic price, be suitable in conjunction with cell engineering directive breeding the strain excellent decomposing Ramulus Ziziphi Spinosae bits again, better effect will be produced.

Claims (10)

1. utilize Ramulus Ziziphi Spinosae to consider a method of cultivating elegant precious mushroom to be worth doing, comprise the steps:
A. the preparation of liquid spawn and cultivation;
B. the preparation of grain spawn and cultivation;
C. the preparation of cultivation medium;
D., under aseptic condition, the medium that the grain spawn that prepared by step B access step C prepares, carries out cultivating, management of producing mushroom, to gather;
It is characterized in that: in steps A or/and step B or/and step C medium in containing Ramulus Ziziphi Spinosae bits.
2. according to claim 1ly utilize Ramulus Ziziphi Spinosae to consider to be worth doing to cultivate the method for elegant precious mushroom, its feature comprises: in steps A elegant precious mushroom liquid spawn culture medium in add Ramulus Ziziphi Spinosae bits in advance as substrate for induction thing; It optimizes liquid spawn culture medium formulation weight percentage: potato 20%, Ramulus Ziziphi Spinosae powder 5%, wheat bran 3%, glucose 2%, KH 2pO 40.3%, MgSO 40.15%, VB110mg/L, carboxymethyl cellulose 0.3%, water 1000mL, pH nature.
3. utilize Ramulus Ziziphi Spinosae to consider the method for cultivating elegant precious mushroom to be worth doing according to claim 2, in described steps A, the preparation of liquid spawn is characterised in that:
A. the preparation of level liquid bacterial classification
Under aseptic condition, commercially available mother is planted in access level liquid medium, 26-28 DEG C, 160r/min constant-temperature shaking culture 5d, i.e. obtained level liquid bacterial classification;
Described level liquid bacterium culture medium comprises: by weight percentage, potato 20%, wheat bran 3%, glucose 2%, KH 2pO 40.3%, MgSO 40.15%, CMC0.3%, VB 110mg/L;
B. the preparation of second-class liquid isolate
Described second-class liquid isolate culture medium prescription percentage by weight is: potato 20%, Ramulus Ziziphi Spinosae powder 5%, wheat bran 3%, glucose 2%, KH 2pO 40.3%, MgSO 40.15%, CMC0.3%, VB 110mg/L.Potato, Ramulus Ziziphi Spinosae bits powder and the wheat bran 1000mL that adds water boil by preparation method altogether, keep micro-and boil 30 minutes, filter, get filtrate, add other component while hot, stir after melting, be settled to water 1000mL, packing, sterilizing after boiling, to be seeded;
Under aseptic condition, by level liquid bacterial classification with in the inoculum concentration access secondary liquid medium of 10%, 26-28 DEG C, 160r/min constant-temperature shaking culture 7d, i.e. obtained second-class liquid isolate.
4. according to claim 1ly utilize Ramulus Ziziphi Spinosae to consider to be worth doing to cultivate the method for elegant precious mushroom, its feature comprises: in step B elegant precious mushroom grain spawn medium in also as substrate for induction thing, add Ramulus Ziziphi Spinosae bits in advance; Its grain spawn culture medium prescription optimized: wheat 84%, Ramulus Ziziphi Spinosae bits 15%, quicklime 1%.
5. utilize Ramulus Ziziphi Spinosae to consider the method for cultivating elegant precious mushroom to be worth doing according to claim 4, in described step B, the preparation of grain spawn is characterised in that:
Second-class liquid isolate access grain spawn medium steps A obtained, inoculum concentration is 10%, 26-28 DEG C and is cultured to full, obtains grain spawn.
6. the Ramulus Ziziphi Spinosae that utilizes according to claim 1 considers the method for cultivating elegant precious mushroom to be worth doing, its feature comprises: in step C, Ramulus Ziziphi Spinosae bits are as the major ingredient of the elegant precious mushroom of cultivation, and culture medium for cultivating weight composition comprises: Ramulus Ziziphi Spinosae bits 60%, cotton seed hull 20%, wheat bran 19%, quicklime 1%.
7. utilize Ramulus Ziziphi Spinosae to consider the method for cultivating elegant precious mushroom to be worth doing according to claim 6, in described step C, the preparation of Ramulus Ziziphi Spinosae bits medium is characterised in that: Ramulus Ziziphi Spinosae, reed powder are broken into wood chip shape, be equipped with cotton seed hull, wheat bran and quicklime, material-water ratio is 1: 1.4, pile vexed 30min, load in container, sterilizing, for subsequent use after cooling.
8. utilize Ramulus Ziziphi Spinosae to consider the method for cultivating elegant precious mushroom to be worth doing according to claim 1, it is characterized in that, in step D, the inoculum concentration of grain spawn is 5-10%.
9. utilize Ramulus Ziziphi Spinosae to consider the method for the elegant precious mushroom of medium cultivation to be worth doing according to claim 2, it is characterized in that, in liquid nutrient medium, Ramulus Ziziphi Spinosae bits used, pulverize and cross 80 mesh sieves in advance.
10. utilize Ramulus Ziziphi Spinosae to consider the method for cultivating elegant precious mushroom to be worth doing according to claim 1, it is characterized in that, in step C, the sterilising conditions of Ramulus Ziziphi Spinosae bits medium is 121 DEG C of autoclaving 150min or 100 DEG C normal-pressure sterilization 24h.
CN201510407772.1A 2015-07-13 2015-07-13 Method for culturing pleurotus geesteranus by using wild-jujube branch sawdust Pending CN105165387A (en)

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CN107434608A (en) * 2016-05-26 2017-12-05 蒋凌辉 A kind of planting material for improving elegant precious mushroom yield
CN107711291A (en) * 2017-10-11 2018-02-23 诸暨马谷亲科技有限公司 A kind of pocket mushroom culture medium for adding catkin and preparation method thereof
CN108718915A (en) * 2018-04-25 2018-11-02 广西壮族自治区农业科学院微生物研究所 Improve the culture medium and cultural method of pleurotus edible fungus yield

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